CN214373750U - Water bath dyeing device - Google Patents
Water bath dyeing device Download PDFInfo
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- CN214373750U CN214373750U CN202120010565.3U CN202120010565U CN214373750U CN 214373750 U CN214373750 U CN 214373750U CN 202120010565 U CN202120010565 U CN 202120010565U CN 214373750 U CN214373750 U CN 214373750U
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Abstract
The utility model discloses a water bath dyeing device, which comprises a water bath kettle and a dyeing box, wherein the top of the dyeing box is provided with a cover body, a bracket is arranged inside the dyeing box, and the dyeing box is arranged in the water bath kettle; the bracket is used for arranging a glass slide carrying thalli and dye liquor, and the water bath is used for accommodating warm water. The utility model discloses a water bath dyeing apparatus carries out water bath constant temperature heating in-process, need not be artificial for too much intervention, convenient operation, and the dye liquor volatilizees fewly, has reduced the dye liquor and has consumeed, can not make toxic component volatilize to the laboratory with contaminated air, with the phenomenon that can not appear on the slide glass solidification caking, this water bath dyeing apparatus not only can be applied to this thallus dye liquor experiment heavily, still can be applied to in other experiments that need water proof water bath, steam bath or need airtight incubation relatively.
Description
Technical Field
The utility model relates to a biology, medical science experimental apparatus technical field, concretely relates to water bath dyeing apparatus.
Background
The mycobacteria have a large amount of lipid components wrapped outside peptidoglycan on the cell wall, so that water-soluble dye liquor is not easy to enter and is difficult to decolor during gram dyeing. In the acid-fast staining process, heating and prolonged staining time are adopted to promote the firm combination of mycolic acid and carbolic acid with reddish yellow, and the combination is difficult to be decolorized by an acid decolorizing agent, so that the acid-fast staining is called as acid-fast staining, and then alkaline methylene blue is used for counterstaining, the mycobacteria still keep red, and other bacteria and other substances in the background are stained blue.
The acid-fast staining procedure of mycobacteria bacteria mainly comprises: firstly, primary dyeing: covering the mycoderm area with carbolic acid reddening staining solution, heating on the outer flame of alcohol lamp for more than 5min to combine the staining solution with mycolic acid in mycobacteria, washing with running water, and sucking water with absorbent paper; secondly, decoloring: dripping hydrochloric acid alcohol solution until no red color is removed, flushing with running water, and sucking water with absorbent paper; thirdly, counterdyeing: dripping alkaline methylene blue dye solution, covering the mycoderm area, flushing with running water after 1min, and sucking water with absorbent paper. Wherein, in the primary dyeing process, attention needs to be paid to observing the volatilization condition of the dye liquor, the dye liquor is supplemented in time, and the dye liquor is prevented from drying up.
In general, the acid-fast staining process of mycobacteria requires heating to bind the stain, however, the above current acid-fast staining method of mycobacteria has the following disadvantages: (1) when the dye is heated in the primary dyeing process, the dye liquor is volatile, and the consumption of the dye liquor is large in the experiment teaching application process; in addition, because the group of dye liquor contains volatile toxic components such as phenol, hydrochloric acid and the like, a large amount of volatile toxic components cause air pollution in a laboratory, and are not beneficial to the health of experiment operators; (2) in the primary dyeing process, a hand-held clamp is required to clamp a glass slide and heat for more than 5min, so that the arm of an experiment operator is easily trapped; (3) in the initial dyeing and heating process, due to the fact that the temperature near the flame of the alcohol lamp is too high, the dye solution is volatilized quickly, and is easy to solidify and agglomerate on a glass slide, and observation of a dyeing result is affected.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a water bath dyeing apparatus for solve among the current mycobacteria bacterium acid-fast dyeing method easy volatilizing of dye liquor, handheld operation inconvenience and dye liquor solidification caking scheduling problem on easily the slide.
The utility model provides a water bath dyeing device, which comprises a water bath kettle and a dyeing box, wherein the top of the dyeing box is provided with a cover body, a bracket is arranged inside the dyeing box, and the dyeing box is placed in the water bath kettle; the bracket is used for placing a glass slide carrying thalli and a dye solution, and the water bath is used for containing warm water.
Preferably, a circle of highest water level line is arranged on the outer wall of the dyeing box and used for limiting the water level of warm water in the water bath pot not to exceed the highest water level line.
Preferably, the support is a hollow support, a plurality of water flowing channels are arranged in the support,
when the slide glass is washed, the washing liquid can flow into the bottom of the staining box from top to bottom through the flowing water channel.
Preferably, a cover body handle is arranged on the cover body.
Preferably, two side handles are respectively arranged on two sides of the dyeing box, and the side handles are arranged above the highest water level line.
Preferably, the bottom of the staining box is provided with a plurality of legs.
Preferably, the staining box is in the shape of a cylinder or a cuboid.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a water bath dyeing device, which adopts a water bath heating method, a glass slide loaded with thalli and dye liquor is laid on a bracket in a dyeing box, the dyeing box is put into a water bath kettle for constant temperature water bath heating until the thalli on the glass slide are dyed, the heating and dyeing process of the water bath dyeing device does not need too much human intervention, the dyeing operation is convenient, and the process of heating by a handheld clamp in the traditional acid-fast dyeing process is avoided; the whole dye liquor is carried out in the closed space of the dyeing box, so that the volatilization of the dye liquor is reduced, namely the consumption of the dye liquor is reduced; even if the dye solution volatilizes, the dye solution is kept in the dyeing box, so that volatile toxic components such as phenol, hydrochloric acid and the like are not volatilized to a laboratory to pollute the air; because the higher humidity is maintained in the water bath heating process, the phenomenon that the dye solution is solidified and agglomerated on the glass slide cannot occur; the waste liquid after washing is kept in the dyeing box, so that the dyeing box is convenient to collect, and the environment pollution caused by the fact that the waste liquid after washing flows into a sewer is avoided. The utility model discloses a water bath dyeing apparatus carries out water bath constant temperature heating in-process, need not artificially intervene too much, convenient operation, and the dye liquor volatilizees fewly, has reduced the dye liquor and has consumeed, can not make toxic component volatilize to the laboratory with contaminated air, the phenomenon of dye liquor solidification caking also can not appear on the slide glass, and this water bath dyeing apparatus not only can be applied to this thallus dye liquor experiment usefulness, still can be applied to in other experiments that need water proof water bath, steam bath or need relative airtight incubation.
Drawings
Fig. 1 is a three-dimensional side view of a dyeing box provided in embodiment 1 of the present invention when a cover is opened;
fig. 2 is a schematic view of an external structure of a dyeing box provided in embodiment 1 of the present invention when a cover body is closed;
fig. 3 is a schematic structural diagram of the water bath dyeing apparatus provided in embodiment 1 of the present invention in an operating state.
Detailed Description
The utility model discloses there are a great deal of drawbacks to the heating method in the current dyeing method, select the method of water-bath heating, to the water-bath heating method of mycobacterium bacterial dyeing improvement design one kind be convenient for heat, dye and be convenient for observe dyeing, decoloration, the special device of counterstain process to solve on the alcohol burner flame heating in-process dye liquor volatile, handheld operation inconvenience and dye liquor solidification caking scheduling problem on easily the slide.
Example 1
Example 1 provides a water bath dyeing apparatus, the structure of which is described in detail below.
Referring to fig. 1 to 3, the water bath dyeing apparatus includes a water bath 1 and a dyeing box 2.
In order to reduce the volatilization of the dye liquor, the top of the dyeing box 2 is provided with a cover body 3, and in order to open or close the cover body 3, a cover body handle 30 is arranged on the cover body 3.
A bracket 4 is arranged in the staining box 2 and is used for placing a glass slide carrying thalli and staining solution,
the dyeing box 2 is arranged in the water bath 1 and is used for containing warm water.
In order to prevent the phenomenon that the dyeing box 2 overturns due to excessive buoyancy caused by excessive water added into the water bath 1, a circle of highest water level line 100 is arranged on the outer wall of the dyeing box 2 and used for limiting the water level of the warm water in the water bath 1 not to exceed the highest water level line 100.
The support 4 is a hollow support, a plurality of water flow channels 40 are arranged in the support, specifically, the water flow channels 40 can be water flow pipelines or water flow holes, and the support 4 can be used for laying glass slides and is convenient to wash. When the slide glass is washed, the washing liquid can flow into the bottom of the staining box 2 from top to bottom through the flowing water channel 40.
In order to facilitate the experimenter to put the dyeing box 2 into the water bath 1 and take the dyeing box out of the water bath, two side handles 20 are respectively arranged at two sides of the dyeing box 2, and the side handles 20 are arranged above the highest water level line 100.
In order to prevent the dyeing box 2 from being directly and tightly attached to the bottom of the water bath 1 and not easy to take out, the bottom of the dyeing box 2 is provided with a plurality of support legs 5, so that a gap is reserved between the dyeing box 2 and the water bath 1 and vacuum cannot be formed.
The dyeing box 2 is in the shape of a cylinder or a cuboid. When the dyeing box 2 is in the shape of a cylinder, the bottom of the dyeing box 2 is provided with three legs 5, and the three legs 5 are distributed in an equilateral triangle.
When the dyeing box 2 is shaped as a rectangular parallelepiped, four legs 5 are respectively provided at four corners of the bottom of the dyeing box 2, as shown in fig. 1 to 3. As a specific embodiment, the dyeing box 2 has a length of 22cm, a width of 15cm and a height of 10cm, and the cover 3 has a length of 22cm, a width of 15cm and a height of 1.5 cm; the highest water level line 100 is 6cm away from the lower edge of the box body of the dyeing box 2; the width of the side handle 20 is 5cm, and the height is 3 cm; the handle 30 of the cover body is 8cm long and 1.5cm wide; the length of the bracket 4 is 18cm, the width is 10cm, and the height is 8 cm; the legs 5 have a height of 1.5 cm.
The dyeing box 2 satisfying the above dimensions can be applied in most of the water baths 1; the support 4 can be dismantled, highly is less than 2 box bodies 2cm of dyeing box, can block the dye liquor spill, satisfies the support 4 of this size simultaneously and can realize that 6 at least wait to dye slide staining simultaneously.
Example 2
step S1: carrying the handle side handles 20 at the two sides of the dyeing box 2 by hands, putting the dyeing box 2 into the water bath pot 1, wherein the water contained in the water bath pot 1 does not exceed the highest water level line 100, and the water bath effect cannot be influenced too little;
step S2: when the water temperature of the water bath pot 1 rises to 70 ℃, the cover body 3 of the dyeing box 2 is opened through the cover body handle 30;
step S3: drying the glass slides to be dyed carrying the inactivated mycobacteria, laying and fixing at least 6 glass slides to be dyed on a bracket 4, dripping dye liquor, covering a cover body 3, and starting timing dyeing until the experimental requirements are met, such as 15 min;
step S4: opening the cover body 3 and washing the glass slide;
step S5: the glass slide, the bracket 4 and the staining box 2 are taken out in sequence, and the waste liquid is poured into a waste liquid collecting container.
Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. A water bath dyeing device, which is characterized by comprising a water bath kettle (1) and a dyeing box (2),
the top of the dyeing box (2) is provided with a cover body (3), a bracket (4) is arranged in the dyeing box (2),
the dyeing box (2) is placed in the water bath pot (1);
the bracket (4) is used for placing a glass slide carrying thalli and a dye solution, and the water bath pot (1) is used for containing warm water.
2. The water bath dyeing apparatus according to claim 1,
the dyeing box is characterized in that a circle of highest water level line (100) is arranged on the outer wall of the dyeing box (2) and used for limiting the water level of warm water in the water bath pot (1) to be not more than the highest water level line (100).
3. The water bath dyeing apparatus according to claim 1,
the bracket (4) is a hollow bracket, a plurality of water flowing channels (40) are arranged in the bracket,
when the slide glass is washed, the washing liquid can flow into the bottom of the staining box (2) from top to bottom through the flowing water channel (40).
4. The water bath dyeing apparatus according to claim 1,
the cover body (3) is provided with a cover body handle (30).
5. The water bath dyeing apparatus according to claim 2,
two sides of the dyeing box (2) are respectively provided with two side handles (20), and the side handles (20) are arranged on the highest water level line (100).
6. The water bath dyeing apparatus according to claim 1,
the bottom of the dyeing box (2) is provided with a plurality of supporting legs (5).
7. The water bath dyeing apparatus according to claim 1,
the dyeing box (2) is cylindrical or cuboid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120010565.3U CN214373750U (en) | 2021-01-05 | 2021-01-05 | Water bath dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120010565.3U CN214373750U (en) | 2021-01-05 | 2021-01-05 | Water bath dyeing device |
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| Publication Number | Publication Date |
|---|---|
| CN214373750U true CN214373750U (en) | 2021-10-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202120010565.3U Active CN214373750U (en) | 2021-01-05 | 2021-01-05 | Water bath dyeing device |
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| CN (1) | CN214373750U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323882A (en) * | 2021-12-31 | 2022-04-12 | 迈克医疗电子有限公司 | Slide glass making devices |
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2021
- 2021-01-05 CN CN202120010565.3U patent/CN214373750U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323882A (en) * | 2021-12-31 | 2022-04-12 | 迈克医疗电子有限公司 | Slide glass making devices |
| CN114323882B (en) * | 2021-12-31 | 2024-03-29 | 迈克医疗电子有限公司 | Slide glass making device |
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