CN214327750U

CN214327750U - Nucleic acid extraction instrument

Info

Publication number: CN214327750U
Application number: CN202120571467.7U
Authority: CN
Inventors: 伍建; 林朋; 樊治江; 易军林; 伍万龙; 敬正鑫
Original assignee: Mygenostics Chongqing Gene Technology Co ltd
Current assignee: Mygenostics Chongqing Gene Technology Co ltd
Priority date: 2021-03-19
Filing date: 2021-03-19
Publication date: 2021-10-01
Anticipated expiration: 2031-03-19

Abstract

The utility model provides a nucleic acid extraction appearance, the on-line screen storage device comprises a base, the top fixedly connected with driving motor of base, driving motor's output fixedly connected with loads the dish, load and set up a plurality of recesses on the dish, every it has the kit, every all to peg graft in the recess all including a plurality of test tubes that are used for depositing the reagent in the kit, fixedly connected with mount on the base, be provided with rabbling mechanism on the mount, rabbling mechanism includes the cylinder of fixed connection in the base top, the top of loading the dish is provided with the mounting panel. The utility model provides a carousel that exists among the prior art when rotating, remaining reagent may fall into in the adjacent test tube of corresponding test tube on the bar magnet cover to and can not detect whether the recess on kit and the carousel aligns the problem of chucking, produced and prevented that remaining reagent from sheathe in the bar magnet and polluting adjacent test tube and be convenient for detect whether the recess on kit and the carousel aligns the effect of chucking.

Description

Nucleic acid extraction instrument

Technical Field

The utility model relates to a medical treatment check out test set technical field especially relates to a nucleic acid extraction appearance.

Background

Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.

When extracting the operation to the nucleic acid in the reagent box of current nucleic acid extraction appearance, it is fixed to put a plurality of reagent boxes that are equipped with different reagents on a carousel chucking mostly, realize the schizolysis of magnetic bead method through the work of carousel rotation and magnetic sleeve stick, combine, washing and processes such as elution, contain a plurality of test tubes in every reagent box, every test tube is inserted the stirring by a corresponding magnetic rod cover respectively, and the carousel is when rotating, remaining reagent probably falls into the test tube that corresponds the test tube in the magnetic rod cover, cause the reagent in the test tube to be contaminated, lead to the experimental result inaccurate, and sometimes the manual work is when putting the reagent box on the carousel, do not align chucking with the recess on kit and the carousel, lead to the magnetic rod cover not to align with the test tube in the reagent box when stirring, damage kit and magnetic rod cover, cause unnecessary loss.

SUMMERY OF THE UTILITY MODEL

To exist not enough among the prior art, the utility model provides a nucleic acid extraction appearance, it has solved the carousel that exists among the prior art when rotating, and remaining reagent may fall into in corresponding the test tube adjacent test tube on the bar magnet cover, causes the in vitro reagent contaminated, can not detect whether the recess on kit and the carousel aligns the problem of chucking.

According to the embodiment of the utility model, a nucleic acid extraction instrument comprises a base, wherein the top of the base is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a loading disc, a plurality of grooves are arranged on the loading disc, reagent boxes are inserted in each groove, each reagent box comprises a plurality of test tubes for storing reagents, a fixing frame is fixedly connected on the base, a stirring mechanism is arranged on the fixing frame, the stirring mechanism comprises a cylinder fixedly connected on the top of the base, a mounting plate is arranged above the loading disc, a plurality of magnetic rod sleeves for stirring and magnetic absorption are fixedly connected on the mounting plate, the output end of the cylinder vertically moves and is fixedly connected with the mounting plate, an electric heating plate is arranged below the loading disc, the electric heating plate is coaxially arranged with the mounting plate, and a liquid baffle plate is rotatably connected on the fixing frame, the liquid baffle plate is positioned above the loading disc and also comprises a detection mechanism for detecting whether the reagent box is completely inserted into the groove.

The technical principle and the beneficial effects of the utility model are that: this appearance is drawed to nucleic acid, keep off the liquid board through setting up, make the loading dish at rotatory in-process, the user can rotate to bar magnet cover below through keeping off liquid board translation, avoid the reagent drippage on the bar magnet cover to the appointed test tube in adjacent test tube, cause the in vitro reagent to be contaminated, simultaneously through the detection mechanism who sets up, make the appearance is drawed to nucleic acid carrying out the during operation, can align the chucking in the recess through detection mechanism detect reagent box, avoid the rabbling mechanism during operation, because of the kit is not correctly counterpointed and lead to the condition emergence of kit and bar magnet cover damage.

Drawings

Fig. 1 is a schematic diagram of an embodiment of the present invention.

Fig. 2 is a side view of an embodiment of the present invention.

Fig. 3 is a top view of an embodiment of the present invention.

In the above drawings: 1. a base; 2. loading a disc; 3. a kit; 4. mounting a plate; 5. a magnetic rod sleeve; 6. a cylinder; 7. an auxiliary support column; 8. a fixed mount; 9. a liquid baffle; 10. an electrical heating plate; 11. a servo motor; 12. a photoelectric sensor A; 13. a photoelectric sensor B; 14. an electric telescopic rod; 15. a head bar; 16. a connecting arm; 17. a connecting plate; 18. and a signal emitter B.

Detailed Description

The technical solution of the present invention will be further explained with reference to the accompanying drawings and embodiments.

As shown in fig. 1 and 2, an embodiment of the present invention provides a nucleic acid extraction apparatus, which includes a base 1, a driving motor is fixedly connected to the top of the base 1, an output end of the driving motor is fixedly connected to a loading tray 2, a plurality of grooves are formed in the loading tray 2, a reagent kit 3 is inserted into each groove, the driving motor is arranged to drive the loading tray 2 to rotate, so that the reagent kit 3 can be automatically switched, each reagent kit 3 includes a plurality of test tubes for storing reagents, specifically, each groove is provided with a top member hole, a top member device is fixedly installed at the top of the base 1, the top member device includes an electric telescopic rod 14 installed at the top of the base 1, an output end of the electric telescopic rod 14 is fixedly connected with a top member rod 15, when in use, after the reagent kit 3 is manually held up, the electric telescopic rod 14 is started to drive the ejector rod 15 to be ejected out of the ejector hole, so that the reagent kit 3 in the inserting groove can be taken out, and the situation that the reagent kit 3 is clamped in the groove and is not convenient to take out due to too much tightness is prevented.

The base 1 is fixedly connected with a fixing frame 8, the fixing frame 8 is provided with a stirring mechanism, specifically, the stirring mechanism and a piece ejecting device are respectively positioned at two sides of the axis of the loading disc 2, the stirring mechanism comprises a cylinder 6 fixedly connected to the top of the base 1, a mounting plate 4 is arranged above the loading disc 2, the mounting plate 4 is fixedly connected with a plurality of magnetic rod sleeves 5 for stirring and magnetic attraction, specifically, when in use, each test tube is respectively inserted into the corresponding magnetic rod sleeve 5 for stirring, the output end of the cylinder 6 vertically moves and is fixedly connected with the mounting plate 4, an electric heating plate 10 is arranged below the loading disc 2, the electric heating plate 10 and the mounting plate 4 are coaxially arranged, specifically, the fixing frame 8 is fixedly connected with a connecting plate 17, the electric heating plate 10 is fixedly connected to the top of the connecting plate 17, as shown in fig. 3, the liquid blocking plate 9 is further rotatably connected to the fixing frame 8, the liquid blocking plate 9 is located above the loading disc 2, specifically, a bolt is connected to the fixing frame 8 through threads, a connecting arm 16 is fixedly connected to the bolt, and the connecting arm 16 is fixedly connected with the liquid blocking plate 9.

The detection mechanism comprises a photoelectric sensor A12 fixed at the bottom of the mounting plate 4 and used for detecting whether the reagent kit 3 completely falls into the groove, and a photoelectric sensor B13 fixed on the fixing frame 8 and used for detecting whether the height of the reagent kit 3 is normal, wherein specifically, a plurality of magnetic rod sleeves 5 enclose a rectangular area, the photoelectric sensor A12 is positioned at the periphery of the rectangular area, the detection mechanism comprises a photoelectric sensor A12 which is fixed at the bottom of the mounting plate 4 and is used for detecting whether the reagent kit 3 completely falls into the groove, a signal emitter a (not shown) for emitting a light sensing signal received by the photoelectric sensor a12 is fixedly installed in the groove of the loading disc 2, the geometric center of the top of the loading disc 2 is fixedly provided with a signal emitter B18 for emitting a light sensing signal received by the photoelectric sensor B13. Still fixedly connected with auxiliary stay post 7 at the 1 top of base, auxiliary stay post 7 is located the below of loading dish 2 and loads dish 2 and separates there is a centimetre interval, auxiliary stay post 7 and the coaxial setting of mounting panel 4, through the auxiliary stay post 7 that sets up, make rabbling mechanism when stirring the operation, auxiliary stay post 7 can carry out auxiliary stay to loading dish 2, prevents to load dish 2 out of balance and leads to the reagent slope in the kit 3 to spill.

When the device is used, a plurality of reagent kits 3 are manually inserted into the grooves of the loading disc 2 in sequence according to the rotating direction and the reaction sequence of the servo motor 11, then the servo motor 11 is started to rotate the loading disc 2 by a fixed angle to enable the first reagent kit 3 to be positioned under the mounting plate 4, the photoelectric sensor A12 detects whether the reagent kits 3 are completely inserted into the grooves to cover signals emitted by the signal emitter A in the grooves, the photoelectric sensor B13 detects whether signals emitted by the signal emitter B18 in the center of the loading disc 2 are completely shielded by the reagent kits 3 to judge whether the height of the reagent kits 3 is normal, after the detection of the photoelectric sensor A12 and the photoelectric sensor B13, the air cylinder 6 drives the mounting plate 4 and the magnetic rod sleeves 5 on the mounting plate 4 to move up and down for stirring and extraction, at the moment, the auxiliary support columns 7 play a role in auxiliary support to prevent the loading disc 2 from losing balance, when in stirring, can start the process that electric heating plate 10 promoted the stirring and draw as required, treat that first kit 3 reaction is accomplished the back, cylinder 6 drives mounting panel 4 and shifts up, and the manual work rotates liquid baffle 9 and moves to mounting panel 4 below, and servo motor 11 is started again and load dish 2 rotates fixed angle and makes second kit 3 be in under mounting panel 4, repeats above-mentioned step until nucleic acid extraction is accomplished.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. The utility model provides a nucleic acid draws appearance, includes the base, the top fixedly connected with driving motor of base, driving motor's output fixedly connected with loading dish, a plurality of recesses have been seted up on the loading dish, every all peg graft in the recess and have the kit, every all include a plurality of test tubes that are used for depositing reagent in the kit, its characterized in that: fixedly connected with mount on the base, be provided with rabbling mechanism on the mount, rabbling mechanism includes fixed connection in the cylinder at base top, the top of loading dish is provided with the mounting panel, a plurality of bar magnet covers that are used for the stirring to inhale the magnetism of fixedly connected with on the mounting panel, the output vertical motion of cylinder and with mounting panel fixed connection, the below of loading dish is provided with the electrical heating board, the electrical heating board sets up with the mounting panel is coaxial, it is connected with the fender liquid board still to rotate on the mount, the fender liquid board is located the top of loading dish, still including being used for the detection mechanism that whether detect reagent box inserts the recess completely.

2. The nucleic acid extractor of claim 1, wherein: the detection mechanism comprises a photoelectric sensor A fixed at the bottom of the mounting plate and used for detecting whether a kit completely falls into the groove or not, and further comprises a photoelectric sensor B fixed on the fixing frame and used for detecting whether the kit height is normal or not, a signal emitter A used for emitting a light sensing signal received by the photoelectric sensor A is fixedly installed in the groove of the loading disc, and a signal emitter B used for emitting the light sensing signal received by the photoelectric sensor B is fixedly installed at the geometric center of the top of the loading disc.

3. The nucleic acid extractor of claim 1, wherein: and each groove is internally provided with a top piece hole.

4. The nucleic acid extractor of claim 3, wherein: the base top fixed mounting has a liftout device, the liftout device is including installing the electric telescopic handle at the base top, electric telescopic handle's output fixedly connected with liftout pole.

5. The nucleic acid extractor of claim 1, wherein: the fixing frame is in threaded connection with a bolt, a connecting arm is fixedly connected to the bolt, and the connecting arm is fixedly connected with the liquid baffle plate.

6. The nucleic acid extractor of claim 1, wherein: the base top is fixedly connected with an auxiliary supporting column, the auxiliary supporting column is located below the loading disc, and the auxiliary supporting column and the mounting plate are coaxially arranged.

7. The nucleic acid extractor of claim 1, wherein: the fixing frame is fixedly connected with a connecting plate, and the electric heating plate is fixedly connected to the top of the connecting plate.