CN214310551U - Western blot antibody elution device - Google Patents
Western blot antibody elution device Download PDFInfo
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- CN214310551U CN214310551U CN202120320653.3U CN202120320653U CN214310551U CN 214310551 U CN214310551 U CN 214310551U CN 202120320653 U CN202120320653 U CN 202120320653U CN 214310551 U CN214310551 U CN 214310551U
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- liquid
- western blot
- incubation box
- antibody elution
- liquid outlet
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Abstract
The utility model relates to a western b l ot antibody elution device, which comprises a liquid storage tank, a water inlet and outlet component, a shaking table component and an incubation box arranged on the shaking table component; the water inlet and outlet assembly comprises a liquid inlet pipe and a liquid outlet pipe; the incubation box is provided with a liquid injection port and a liquid outlet; the liquid injection port is connected with the liquid storage tank through a liquid inlet pipe, and the liquid outlet is connected with a liquid outlet pipe; a liquid level sensor for detecting the liquid level is additionally arranged in the incubation box; a first adjusting valve is arranged in the liquid inlet pipe, and a second adjusting valve is arranged in the liquid outlet pipe. The liquid injection device can automatically inject liquid into the incubation box and automatically discharge the liquid in the incubation box. Repeated steps of operation of scientific researchers in the Western B shot experiment are reduced, the operation is completed by using the antibody elution device, the operation time and labor cost are reduced, and the consumption of reagents is reduced.
Description
Technical Field
The utility model belongs to the technical field of biological laboratory equipment and specifically relates to a western blot antibody elution device.
Background
The western blot experiment is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. Accurate, simple and rapid experimental operation in western blot experiments is always pursued by experimenters.
The current western blot experimental process is generally carried out by manual step operation. When the experimenter injects liquid into the incubation box and discharges the liquid in the incubation box, the experimenter needs manual operation, and the process is complicated and consumes a long time.
Therefore, it is necessary to design a new western blot antibody elution apparatus.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a western blot antibody elution device, it can carry out the automation to annotate in hatching the box and carry out the automatic liquid operation to the liquid in hatching the box. Repeated steps of operation of scientific researchers in the Western Blot experiment when eluting redundant antibodies are reduced, the operation is completed by using the antibody elution device, the operation time and labor cost are reduced, and the consumption of reagents is reduced.
In order to solve the technical problem, the utility model provides a western blot antibody elution device, which comprises a liquid storage tank, a water inlet and outlet component, a shaking table component and an incubation box arranged on the shaking table component; the water inlet and outlet assembly comprises a liquid inlet pipe and a liquid outlet pipe; the incubation box is provided with a liquid injection port and a liquid outlet; the liquid injection port is connected with the liquid storage tank through a liquid inlet pipe, and the liquid outlet is connected with a liquid outlet pipe; a liquid level sensor for detecting the liquid level is additionally arranged in the incubation box; the liquid inlet pipe is internally provided with a first regulating valve for controlling liquid inlet, and the liquid outlet pipe is internally provided with a second regulating valve for controlling liquid outlet.
Preferably, the shaking table assembly comprises a table frame and a shaking platform arranged at the end part of the table frame, and the shaking platform is provided with a discharging unit used for discharging the incubation box at one time.
Preferably, the blanking unit comprises a driving motor and a plurality of groups of guide rails which are arranged on the oscillating platform in a protruding manner, and a push block is arranged on the guide rails in a sliding manner; the driving motor is electrically connected with the push block to drive the push block to move relative to the guide rail.
Preferably, an oscillating station for placing the incubation box is formed between two adjacent guide rails.
Preferably, a displacement sensor for detecting the position of the incubation box is additionally arranged on the oscillation platform.
Preferably, the western blot antibody elution device comprises a driving component for driving the oscillating platform to horizontally oscillate; the driving assembly comprises a driving shaft and a speed regulating motor; the adjustable speed motor is arranged on the rack, one end of the driving shaft is connected with the rack, the other end of the driving shaft is connected with the vibration platform, and the adjustable speed motor is electrically connected with the driving shaft to drive the vibration platform to vibrate.
Preferably, a timer for controlling oscillation time is arranged on the oscillation platform, and the timer is electrically connected with the speed regulating motor.
Preferably, the liquid injection port is located at the upper part of the incubation box, and the liquid outlet is located at the lower part of the incubation box.
Preferably, the incubation box is connected with a first sealing plug and a second sealing plug which can be respectively matched with the liquid injection port and the liquid outlet.
Preferably, the first sealing plug comprises a convex strip which is inserted into the liquid inlet, and the end part of the convex strip is provided with a circular truncated cone structure; and a silica gel gasket for enhancing the sealing performance is arranged on the periphery of the raised line.
Compared with the prior art, the technical scheme of the utility model have following advantage:
the utility model discloses a set up business turn over water subassembly, business turn over water subassembly includes feed liquor pipe and drain pipe. The incubation box is connected with the liquid storage tank through a liquid inlet pipe, and a liquid outlet is arranged on the incubation box and connected with the liquid outlet pipe. The liquid inlet and the liquid outlet can be respectively controlled through the first regulating valve and the second regulating valve. Compared with the traditional western blot, the method does not need manual liquid feeding and discharging. The liquid injection device can automatically inject liquid into the incubation box and automatically discharge liquid from the incubation box.
Drawings
In order to make the content of the present invention more clearly understood, the present invention will be described in further detail with reference to the following embodiments of the present invention, in conjunction with the accompanying drawings, wherein:
FIG. 1 is a schematic view of the structure of a cradle assembly of the present invention;
fig. 2 is a schematic view of the connection among the middle liquid storage tank, the water inlet and outlet assembly and the incubation box of the present invention.
The specification reference numbers indicate: 10-a rack, 11-an oscillating platform, 12-a displacement sensor, 13-a pushing block, 14-a driving motor, 15-a guide rail, 16-an oscillating station, 17-a timer, 18-a control button, 19-a display screen, 21-a liquid storage tank, 22-a liquid inlet pipe, 23-a first adjusting valve, 24-an incubation box, 25-a second adjusting valve, 26-a liquid outlet pipe and 27-a waste liquid tank.
Detailed Description
The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.
Referring to fig. 1-2, the utility model discloses a western blot antibody elution device, include:
a liquid storage tank 21, a water inlet and outlet component, a waste liquid tank 27, a shaking table component and an incubation box 24 arranged on the shaking table component.
Specifically, the upper portion of the incubation box 24 is provided with a liquid injection port, and the lower portion of the incubation box 24 is provided with a liquid outlet. The water inlet and outlet assembly includes an inlet pipe 22 and an outlet pipe 26. One end of the liquid inlet pipe 22 is inserted into the liquid injection port, the other end of the liquid inlet pipe 22 is connected with the liquid storage tank 21, and a first regulating valve 23 capable of regulating the liquid flow in the liquid inlet pipe 22 is arranged in the liquid inlet pipe 22.
One end of the liquid outlet pipe 26 is inserted into the lower part of the incubation box 24, the other end of the liquid outlet pipe 26 is connected with a waste liquid tank 27, and a second regulating valve 25 capable of regulating the flow rate of the liquid in the liquid outlet pipe 26 is arranged in the liquid outlet pipe 26. The first regulating valve 23 and the second regulating valve 25 are both solenoid valves.
Preferably, the liquid inlet pipe 22 and the liquid outlet pipe 26 are flexible pipes, and the lengths of the liquid inlet pipe 22 and the liquid outlet pipe 26 can be adjusted according to needs. A liquid level sensor for detecting the liquid level is arranged in the incubation box 24, and a singlechip for controlling is also arranged in the incubation box 24. The first regulating valve 23, the second regulating valve 25 and the liquid level sensor are all electrically connected with the single chip microcomputer.
Preferably, the liquid inlet and the liquid outlet can be controlled by controlling the first adjusting valve 23 and the second adjusting valve 25, respectively. Compared with the traditional western blot, the method does not need manual liquid feeding and discharging. Which can automatically inject liquid into the incubation box 24 and automatically discharge liquid from the incubation box 24.
Further, the incubation box 24 is further connected with a first sealing plug and a second sealing plug, the first sealing plug can be connected with the liquid injection port, and the second sealing plug can be connected with the liquid outlet. The first sealing plug is the same shape as the second sealing plug. After the antibody elution operation is completed, the first sealing plug can be matched with the liquid injection port, and the second sealing plug can be matched with the liquid outlet port. Above-mentioned first sealing plug includes the sand grip and adds the round platform structure at the sand grip tip, and above-mentioned sand grip can insert and establish in the inlet port, and the surface cladding of above-mentioned sand grip has a silica gel packing ring, and it can strengthen the sealing performance between sand grip and the inlet.
The shaking table assembly comprises a table frame 10 and a shaking platform 11 arranged on the table frame 10, and the incubation box 24 is arranged on the shaking platform 11.
Preferably, a driving assembly is added between the oscillating platform 11 and the rack 10, and the driving assembly includes a driving shaft, a speed-regulating motor and a timer 17. Preferably, the driving shafts are provided with a plurality of driving shafts, one end of each driving shaft is connected with the rack 10, the other end of each driving shaft is connected with the oscillating platform 11, and the speed regulating motor is arranged on the rack 10. The speed regulating motor is electrically connected with the driving shaft to drive the oscillating platform 11 to oscillate. The timer 17 is electrically connected to the speed regulating motor for controlling the oscillation time of the oscillation platform 11.
The oscillating platform 11 is provided with a blanking unit capable of blanking the incubation box 24 once. The blanking unit comprises a driving motor 14 and a plurality of groups of guide rails 15 which are arranged on the oscillating platform 11 in a protruding mode. Each group of guide rails 15 comprises two guide rails 15 which are parallel to each other, and each group of guide rails 15 is provided with a push block 13 in a sliding manner. The driving motor 14 is electrically connected to the pushing block 13 to drive the pushing block 13 to move relative to the guide rail 15.
An oscillating station 16 for placing an incubation box 24 is formed between two adjacent guide rails 15. Above-mentioned incubation box 24 is placed on shaking station 16, and the guide rail 15 that above-mentioned protrusion set up can carry on spacingly to incubation box 24, avoids shaking the in-process and incubate box 24 and drop.
The displacement sensor 12 is added on the oscillation platform 11, and can detect the position of the incubation box 24 in real time. After the antibody elution is completed, the incubation box 24 needs to be fed once, the driving motor 14 is started to drive the push block 13 to move relative to the guide rail 15, and the running speed of the push block 13 is adjustable. A bearing plate is arranged beside the oscillating platform 11, and the push block 13 can discharge the incubation box 24 on the oscillating platform 11 onto the bearing plate at one time. Compared with the traditional elution device, the incubation boxes 24 do not need to be taken out one by one manually, the experiment time is saved, and the automation degree is higher.
Preferably, a display screen 19 capable of displaying various parameters during the operation of the elution device and a control button 18 for controlling the operation of the elution device are provided on the side wall of the rack 10.
The working principle is as follows: the incubation cassette 24 is first placed on the oscillation station 16 in the shaker assembly. The liquid injection port of the incubation box 24 is connected with the liquid storage tank 21 through a liquid inlet pipe 22, the liquid outlet of the incubation box 24 is connected with the waste liquid tank 27 through a liquid outlet pipe 26, and the lengths of the liquid inlet pipe 22 and the liquid outlet pipe 26 are adjusted according to actual requirements. The first control valve and the second control valve 25 are used for automatically injecting liquid into the incubation box 24 and automatically discharging liquid from the incubation box 24.
Then, shake the platform 11 among the shaking table subassembly and can drive and hatch box 24 and vibrate, shake and be provided with the unloading subassembly on the platform 11. After the oscillation is completed, the incubation box 24 is subjected to one-time blanking: the driving motor 14 is started, a bearing plate is arranged beside the oscillating platform 11, and the push block 13 can discharge the incubation box 24 on the oscillating platform 11 onto the bearing plate at one time.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious changes and modifications can be made without departing from the scope of the invention.
Claims (10)
1. A western blot antibody elution apparatus, comprising:
the device comprises a liquid storage tank, a water inlet and outlet component, a shaking table component and an incubation box arranged on the shaking table component;
the water inlet and outlet assembly comprises a liquid inlet pipe and a liquid outlet pipe; the incubation box is provided with a liquid injection port and a liquid outlet; the liquid injection port is connected with the liquid storage tank through a liquid inlet pipe, and the liquid outlet is connected with a liquid outlet pipe;
a liquid level sensor for detecting the liquid level is additionally arranged in the incubation box; the liquid inlet pipe is internally provided with a first regulating valve for controlling liquid inlet, and the liquid outlet pipe is internally provided with a second regulating valve for controlling liquid outlet.
2. The western blot antibody elution device according to claim 1, wherein the shaking table assembly comprises a table frame and a shaking platform arranged at the end of the table frame, and the shaking platform is provided with a feeding unit for feeding the incubation box at one time.
3. The western blot antibody elution device according to claim 2, wherein the blanking unit comprises a driving motor and a plurality of groups of guide rails which are arranged on the oscillating platform in a protruding manner, and a push block is arranged on the guide rails in a sliding manner; the driving motor is electrically connected with the push block to drive the push block to move relative to the guide rail.
4. The western blot antibody elution device according to claim 3, further comprising a shaking station for placing an incubation box, wherein the shaking station is located between the two guide rails.
5. The western blot antibody elution apparatus according to claim 2, wherein a displacement sensor for detecting the position of the incubation box is added on the oscillating platform.
6. The western blot antibody elution apparatus according to claim 2, comprising a driving assembly for driving the oscillating platform to oscillate horizontally; the driving assembly comprises a driving shaft and a speed regulating motor; the adjustable speed motor is arranged on the rack, one end of the driving shaft is connected with the rack, the other end of the driving shaft is connected with the vibration platform, and the adjustable speed motor is electrically connected with the driving shaft to drive the vibration platform to vibrate.
7. The western blot antibody elution device according to claim 6, wherein a timer for controlling the oscillation time is disposed on the oscillation platform, and the timer is electrically connected to the speed-adjustable motor.
8. The western blot antibody elution device according to claim 1, wherein said liquid injection port is located at an upper portion of the incubation chamber, and said liquid outlet port is located at a lower portion of the incubation chamber.
9. The western blot antibody elution device according to claim 8, wherein the incubation box is connected with a first sealing plug and a second sealing plug which can be respectively matched with the liquid injection port and the liquid outlet port.
10. The western blot antibody elution apparatus according to claim 9, wherein the first sealing plug comprises a convex strip for being inserted into the liquid inlet, and the end of the convex strip has a truncated cone structure; and a silica gel gasket for enhancing the sealing performance is arranged on the periphery of the raised line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120320653.3U CN214310551U (en) | 2021-02-04 | 2021-02-04 | Western blot antibody elution device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120320653.3U CN214310551U (en) | 2021-02-04 | 2021-02-04 | Western blot antibody elution device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214310551U true CN214310551U (en) | 2021-09-28 |
Family
ID=77833173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120320653.3U Active CN214310551U (en) | 2021-02-04 | 2021-02-04 | Western blot antibody elution device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214310551U (en) |
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2021
- 2021-02-04 CN CN202120320653.3U patent/CN214310551U/en active Active
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