CN214300192U - Culture vessel that acellular allogeneic nerve repair material used - Google Patents

Abstract

The utility model discloses a culture vessel that decellularization allogeneic nerve repair material used, including vessel and dispersion portion: the container body comprises a lower end part and an upper end part, and the lower end part is provided with an accommodating space and is detachably and tightly connected with the upper end part; the dispersing part is arranged in the lower end part and comprises a plurality of dispersing columns which are opposite to the inner bottom surface of the lower end part and are convex. An object of the utility model is to provide a can carry out the container of decentralized culture to nerve cell.

Description

Culture vessel that acellular allogeneic nerve repair material used

Technical Field

The utility model belongs to the field of medical equipment, especially, relate to a remove culture vessel that cell allogeneic nerve repair material used.

Background

In traumatic injuries caused by various accidents and natural disasters, peripheral nerves are often damaged or cut off. The surgical repair and cutting of the nerve to be repaired can bring extra trauma and cost, and the cell-free allogeneic nerve repair material can well solve the problem.

In order to obtain excellent acellular allogeneic nerve repair materials in batches and efficiently, the decentralized culture of nerve cells is very important in the process of culturing the nerve cells. If a large lump of aggregated cells is used for the culture, nutrient supply and metabolic waste discharge of the cells located inside thereof are difficult. As such, their survival and growth in culture vessels is relatively difficult. If large-scale proliferation of cells is required, one of the properties needs to be removed and the cells need to be dispersed as much as possible during the culture. However, in the field of preparing acellular allogeneic nerve repair materials, at present, no culture vessel capable of efficiently performing dispersed culture on nerve cells exists.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a vessel that can perform a decentralized culture of nerve cells.

This patent scheme provides a culture vessel that acellular allogeneic nerve repair material used, includes: container body and dispersing part: the container body comprises a lower end part and an upper end part, and the lower end part is provided with an accommodating space and is detachably and tightly connected with the upper end part; the dispersing part is arranged in the lower end part and comprises a plurality of dispersing columns which are opposite to the inner bottom surface of the lower end part and are convex.

Further, the dispersion column is provided on an inner bottom surface of the lower end portion.

Further, the dispersing part further comprises a bottom plate, the dispersing columns are arranged on the bottom plate, and the dispersing part is detachably arranged in the lower end part.

Furthermore, the dispersion columns are circumferentially arranged around the center of the inner bottom surface of the lower end part, and a plurality of circles are arranged from outside to inside.

Further, the diameter of the dispersion column is between 4 and 6mm, the height of the dispersion column is between 8 and 12mm, and the number of the outermost circles is between 14 and 18.

Further, the container body is a bottle, and the upper end portion is a bottle body.

Further, the container body is a conical flask, and the upper end part is a flask body of the conical flask.

Further, the outer diameter of the bottle body of the container body is between 125 and 140mm, the outer diameter of the bottle neck is between 38 and 46mm, the total height is between 210 and 230mm, the minimum wall thickness is between 1 and 1.6mm, the height of the lower end part is between 35 and 45mm, and the height of the upper end part is between 170 and 190 mm.

Further, the upper end part is a sealing cover.

Further, the height of the lower end part is between 35 and 45mm, and the diameter of the sealing cover is between 110 and 130 mm.

The improvement of this patent brings the following advantage:

(1) the utility model provides a culture vessel that cell-free allogeneic nerve repair material used, the bottom of this culture vessel is provided with the dispersion portion of constituteing by a plurality of dispersion posts. The dispersion columns arranged at the bottom can properly and effectively isolate and disperse the nerve cells to be cultured, prevent the nerve cells from agglomerating into a larger cell mass, enlarge the total contact area of the cells and the culture solution, facilitate all the nerve cells to absorb nutrition from the surrounding culture solution, and discharge metabolic waste to the culture solution. Promotes the survival growth and division proliferation of nerve cells, and provides a good resource foundation for efficiently obtaining excellent acellular allogeneic nerve repairing materials in batches.

(2) This dispersion column among culture vessel when with the nerve cell dispersion, does not separate into in one individual check or space with nerve cell and culture solution, and the culture solution still can freely circulate in the container bottom, and the free circulation of culture solution can let the culture solution of each department keep the general unanimity in the container, is favorable to the uniformity and the standardization of nerve cell culture, improves the yields. If the nerve cells and the culture solution are separated into separate grids or spaces, the culture solution in each place has a large difference due to the metabolic activity of the nerve cells, and further the survival, growth, division and proliferation of the nerve cells are influenced, which is not favorable for the consistency and standardization of the nerve cells.

(3) In addition, the container body in the culture container is divided into an upper end part and a lower end part, the upper end part and the lower end part can be tightly connected to form a non-leakage container, and the upper end part can be independently disassembled to be replaced by different upper end parts, so that the culture container has different functions. Greatly expands the application range of the culture container and achieves multiple purposes.

(4) As a further improvement, the dispersion column has two arrangements. One is directly arranged on the inner bottom surface of the lower end part. The second is arranged on the bottom plate to form a detachable dispersing part. Or after the dispersing part is taken out of the container body, the container body can be used as a common container; or the dispersing part is installed in the lower end part, and the culture vessel is a culture vessel which can perform dispersion culture on nerve cells.

(5) As an improvement, the container body has two forms, one is a bottle, in particular a conical bottle; the other is a sealed storage container. In these two forms, the lower end is not changed, and the form of the container body can be changed only by replacing the different upper end, so that the culture container can expand different functions. When the upper end part is the body of the conical flask, the container body is the conical flask, which is beneficial to the scattered culture of the nerve cells by the culture container. When the upper end part is the sealing cover, the container body is a sealed storage container and can be used as a nerve storage vessel for sealed storage.

Drawings

FIG. 1 is a schematic perspective view of a culture vessel for acellular allogeneic nerve repair material according to an embodiment of the present application, and is also a schematic perspective view of the culture vessel when the vessel body is in the form of a conical flask;

fig. 2 is a schematic perspective view of a lower end portion and a dispersing portion in an embodiment of the present application;

fig. 3 is a schematic perspective view of a dispersing part in an embodiment of the present application;

FIG. 4 is a schematic perspective view of the embodiment of the present application when the upper end is a body of a conical flask;

FIG. 5 is a schematic perspective view of a culture container in another embodiment of the present invention, the culture container being in the form of a sealed storage container;

FIG. 6 is a schematic perspective view of a sealing cap according to another embodiment of the present application;

wherein, 1: a container body; 11: a lower end portion; 12: an upper end portion; 12 a: a conical flask body; 12 b: a sealing cover; 2: a dispersing section; 21: a dispersion column; 22: a base plate.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The present invention can also be implemented or applied through other different specific embodiments, and various details in the present specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

Referring to fig. 1 to 6, a culture container for a decellularized allogenic nerve repair material according to an embodiment of the present invention includes a container body 1 and a dispersing unit 2 disposed in the container body 1.

As shown in fig. 1, the container body 1 is a bottle, preferably a conical bottle, and includes a detachable lower end 11 and an upper end 12, and the lower end 11 and the upper end 12 are made of a transparent material. The total height of the container body 1 is 210 mm and 230mm, preferably 220.0 +/-4.0 mm; the minimum wall thickness is 1-1.6mm, preferably 1.3 mm.

As shown in fig. 2, the lower end 11 is the lower end of the bottle of the erlenmeyer flask, and has a containing space, which is like a bowl and has an upward opening, and a certain amount of objects can be stored separately. The height of the lower end 11 is 35-45mm, preferably 40 mm.

As shown in fig. 4, the upper end portion 12 is a substantially tubular body 12a of a conical flask, which is hollow and open at both ends. The upper end part 12 and the lower end part 11 are detachably and tightly connected together to form a conical bottle. The tight connection here means: the upper end portion 12 and the lower end portion 11 are connected to form a container, and the container does not leak at the connection. The detachable connection may be a threaded connection, an interference fit, or other preferred detachable connection that does not leak. The height of the upper end portion 12 is 170-190mm, preferably 180 mm; the outer diameter of the bottle body is 125-140mm, preferably 131.0 +/-3.0 mm; the outer diameter of the bottle neck is 38-46mm, preferably 42.0 +/-2.0 mm.

As shown in fig. 3, the dispersing section 2 includes a bottom plate 22 and a plurality of dispersing columns 21 provided on the bottom plate 22, where a plurality means two or more. The dispersing section 2 is detachably mounted on the inner bottom surface of the lower end portion 11, and the dispersing column 21 is upwardly protruded with respect to the inner bottom surface of the lower end portion 11. The dispersion columns 21 are circumferentially arranged around the center of the inner bottom surface of the lower end portion 11, and a plurality of circles are arranged from outside to inside, and each circle is a concentric circle taking the center point of the inner bottom surface of the lower end portion 11 as the center. The bottom plate 22 is a dish-like container. The dispersing section 2 is provided directly on the inner bottom surface of the lower end portion 11. The diameter of the dispersion column 21 is 4-6mm, preferably 5 mm; the height is 8-12mm, preferably 10 mm; the number of dispersion columns 21 in the outermost circle is 14 to 18, preferably 16.

In another embodiment of the present application, the dispersion post 21 is provided directly on the inner bottom surface of the lower end portion 11.

As shown in fig. 5 and 6, in another embodiment of the present application, the upper end portion 12 is a sealing cover 12b, which forms a sealed storage container with the lower end portion 11 when the upper end portion 12 is connected with the lower end portion 11. The height of the lower end part 11 is 35-45mm, preferably 40 mm; the diameter of the sealing cover 12b is 110-130mm, preferably 120 mm.

In another embodiment of the present application, the bottom plate 22 is a flat plate, and the dispersing part 2 is detachably coupled to the lower end part 11.

In another embodiment of the present application, when the container body 1 is a conical bottle, a bottle cap is further disposed at the bottle mouth, and the diameter of the bottle cap is 50-70mm, preferably 60 mm.

In another embodiment of the present application, the container body 1 and the dispersing part 2 are both made by 3D printing using a copolymer of methyl methacrylate and hydroxyethyl acrylate. For cell culture, the substrates attached to the bottom of the culture dish are generally: polylysine, extracellular matrix components, cell adhesion molecules, a monolayer of non-neuronal cells. The culture conditions of the nerves are generally 37 ℃, 95% of air and 5% of carbon dioxide, the pH value is 7.2-7.4, the osmotic pressure is 260-320 mOsm/kg, and the nerves have basic nutrient substances and no pollution environment. In order to meet the above conditions, a general nerve culture vessel is made of glass or disposable plastic.

Both polymethyl methacrylate (PMMA) and polyhydroxyethyl methacrylate (PHEMA) in the acrylate resin material can be used for repairing the nerve defect. Polymethyl methacrylate (PMMA) has excellent optical properties, weather resistance, electrical insulation, good physical and mechanical properties, chemical stability, and good processability, especially has excellent biocompatibility, but is poorly hydrophilic. The Polyhydroxyethylmethacrylate (PHEMA) belongs to acrylate and has the property of alcohol monomers, and because the side group of each structural unit of the polymer contains hydroxyl, the Polyhydroxyethylmethacrylate (PHEMA) has certain hydrophilicity and shows good biocompatibility. Wherein the inside of the polyhydroxyethyl methacrylate hydrogel is in a three-dimensional network structure, and low molecular weight substances (such as water, oxygen, carbon dioxide, salts, amino acid and the like) can freely penetrate through the hydrogel.

Methyl methacrylate and hydroxyethyl acrylate are copolymerized in the 3D printing process, the performance of the copolymer can be regulated, the tensile strength of the copolymer is gradually reduced along with the increase of the proportion of the monomer hydroxyethyl methacrylate, the elongation at break is increased, the hydrophilicity is improved, and the culture container in the best state can be prepared.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention should be covered by the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A culture container for acellular allogeneic nerve repair materials is characterized by comprising a container body and a dispersing part; the container body comprises a lower end part and an upper end part, and the lower end part is provided with an accommodating space and is detachably and tightly connected with the upper end part; the dispersing part is arranged in the lower end part and comprises a plurality of dispersing columns which are opposite to the inner bottom surface of the lower end part and are convex.

2. The culture vessel for acellular allogeneic nerve repair material according to claim 1, wherein the dispersion column is disposed on an inner bottom surface of the lower end portion.

3. The culture vessel for acellular allogeneic nerve repair material according to claim 1, wherein the dispersion part further includes a bottom plate, the dispersion column is disposed on the bottom plate, and the dispersion part is detachably disposed in the lower end part.

4. The culture vessel for the acellular allogeneic nerve repair material according to any one of claims 1 to 3, wherein the dispersion columns are arranged circumferentially around the center of the inner bottom surface of the lower end part and are provided with a plurality of turns from outside to inside.

5. The culture vessel for the decellularized allogeneic nerve repair material according to claim 4, wherein the diameter of the dispersion column is between 4-6mm, the height is between 8-12mm, and the number of the outermost circles is between 14-18.

6. The culture vessel for acellular allogeneic nerve repair material according to claim 1, wherein the vessel body is a bottle, and the upper end portion is a bottle body.

7. The culture vessel for acellular allogeneic nerve repair material according to claim 6, wherein the vessel body is a conical flask, and the upper end part is a body of the conical flask.

8. The culture container for acellular allogeneic nerve repair material according to claim 7, wherein the outer diameter of the body of the container is between 125 and 140mm, the outer diameter of the neck of the bottle is between 38 and 46mm, the total height is between 210 and 230mm, the minimum wall thickness is between 1 and 1.6mm, the height of the lower end part is between 35 and 45mm, and the height of the upper end part is between 170 and 190 mm.

9. The culture vessel for acellular allogeneic nerve repair material according to claim 1, wherein the upper end portion is a sealing cover.

10. The culture vessel for acellular allogeneic nerve repair material according to claim 9, wherein the height of the lower end part is between 35-45mm, and the diameter of the sealing cover is between 110-130 mm.

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