CN219537516U

CN219537516U - Tubular three-dimensional cell degradable culture bracket

Info

Publication number: CN219537516U
Application number: CN202223562601.0U
Authority: CN
Inventors: 赵钢; 李子洋; 王明杰; 张悦; 李兴杰
Original assignee: Changzhou Juxin Biotechnology Co ltd; Henan Yingwen Information Technology Co ltd; Suzhou Century Coordinate Biotechnology Co ltd
Current assignee: Changzhou Juxin Biotechnology Co ltd; Henan Yingwen Information Technology Co ltd; Suzhou Century Coordinate Biotechnology Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-08-18
Anticipated expiration: 2032-12-30

Abstract

The utility model provides a tubular three-dimensional cell degradable culture support which comprises a cylindrical tube support, wherein a plurality of irregularly arranged circular grooves are formed in the outer surface of the cylindrical tube support, and a plurality of irregularly arranged circular grooves are formed in the inner surface of the cylindrical tube support. The utility model adopts the tubular stereo cell culture bracket built by Polycaprolactone (PCL) material, which can fully simulate the growth of stereo cells, such as in vitro culture experiments of tubular organs such as blood vessels, nerve tubes and the like. Meanwhile, PCL is a degradable material, is nontoxic, generates carbon dioxide and water after degradation, has good biocompatibility, and is widely used in the field of biology. The cell three-dimensional tubular bracket uses degradable PCL material as a matrix, cells can grow and expand on the bracket with holes to form tubular cell tissue substances, and the characteristics of human body tubular tissues can be better simulated.

Description

Tubular three-dimensional cell degradable culture bracket

Technical Field

The utility model relates to the technical field of cell culture, in particular to a tubular three-dimensional cell degradable culture bracket.

Background

At present, cells, especially stem cells, tumor cells and the like need to be subjected to adherent culture, and the cells belong to single-dimension plane culture, so that the characteristics of the cells organized in the actual body are hardly simulated.

Disclosure of Invention

The object of the present utility model is to solve at least one of the technical drawbacks.

Therefore, an object of the present utility model is to provide a tubular three-dimensional cell degradable culture scaffold, which solves the problems mentioned in the background art and overcomes the defects existing in the prior art.

In order to achieve the above purpose, the utility model provides a tubular three-dimensional cell degradable culture support, which comprises a cylindrical tube support, wherein a plurality of irregularly arranged circular grooves are formed in the outer surface of the cylindrical tube support, and a plurality of irregularly arranged circular grooves are formed in the inner surface of the cylindrical tube support.

Preferably, the cylindrical pipe body bracket is made of polycaprolactone PLC material.

In any of the above embodiments, it is preferable that the thickness of the wall of the cylindrical stent is 1mm.

In any of the above embodiments, it is preferable that the cylindrical tube body support has a cylindrical tubular structure.

Compared with the prior art, the utility model has the following advantages and beneficial effects:

1. the tubular three-dimensional cell degradable culture bracket is constructed by PCL material, and can fully simulate the growth of three-dimensional cells, such as in-vitro culture experiments of tubular organs such as blood vessels, neural tubes and the like. Meanwhile, PCL is a degradable material, is nontoxic, generates carbon dioxide and water after degradation, has good biocompatibility, and is widely used in the field of biology. The cell three-dimensional tubular bracket uses degradable PCL material as a matrix, cells can grow and expand on the bracket with holes to form tubular cell tissue substances, and the characteristics of human body tubular tissues can be better simulated.

2. The utility model relates to a tubular three-dimensional cell degradable culture bracket which adopts a cylindrical tubular body with holes, wherein the round holes are irregularly arranged on the tube wall, the thickness of the tubular body is 1mm, the length of the tubular body can be designed according to requirements, and the tubular body is integrally made of PCL. The round groove can better enable the cells to form three-dimensional culture on the bracket, and simultaneously improve the mutual connection of the cells, thereby being capable of better carrying out three-dimensional culture

Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The foregoing and/or additional aspects and advantages of the utility model will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

fig. 1 is a front view of a tubular three-dimensional cell degradable culture scaffold according to an embodiment of the utility model.

FIG. 2 is a left side view of a tubular three-dimensional cell degradable culture scaffold according to an embodiment of the utility model

Wherein: 1-a cylindrical pipe body bracket; 2-round grooves.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

As shown in fig. 1 and fig. 2, a tubular three-dimensional cell degradable culture support according to an embodiment of the utility model comprises a cylindrical tube support 1, wherein a plurality of irregularly arranged circular grooves 2 are formed in the outer surface of the cylindrical tube support, and a plurality of irregularly arranged circular grooves are formed in the inner surface of the cylindrical tube support.

The circular groove on the outer surface of the cylindrical support of the support is not opposite to the circular groove on the inner surface of the cylindrical support, so that the circular groove on the outer wall is prevented from being opposite to the circular groove on the inner wall, and the thickness of the tubular body is prevented from being excessively reduced.

The tubular three-dimensional cell degradable culture bracket provided by the embodiment of the utility model is a tubular three-dimensional cell culture bracket built by PCL materials, and can fully simulate the growth of three-dimensional cells, such as in-vitro culture experiments of tubular organs such as blood vessels, neural tubes and the like. Meanwhile, PCL is a degradable material, is nontoxic, generates carbon dioxide and water after degradation, has good biocompatibility, and is widely used in the field of biology. The cell three-dimensional tubular bracket uses degradable PCL material as a matrix, cells can grow and expand on the bracket with holes to form tubular cell tissue substances, and the characteristics of human body tubular tissues can be better simulated.

Furthermore, the cylindrical pipe body bracket is made of polycaprolactone PLC material.

Further, the thickness of the tube wall of the cylindrical support is 1mm.

Further, the cylindrical pipe body support is of a cylindrical tubular structure.

The using method comprises the following steps: and (3) culturing cells, namely uniformly mixing 10X105 cells of stem cells to be cultured with a culture medium added with fetal calf serum, adding the mixture into a culture dish, placing a tubular bracket into the culture dish, adding enough complete culture medium into the culture dish, guiding the whole bracket to be completely submerged, shaking the culture dish left and right for 2 minutes, and enabling the stem cells to be fully contacted with the bracket and attached to the pipe wall.

Culturing in a cell culture box under the following conditions: 37 ℃,5% CO2,5% humidity

Every 3 days of culture, the culture medium is changed, after 15 days, the cells can be observed to grow and expand on the wall of the bracket, after 30 days, PCL is basically degraded, and finally the tubular-like cell tissue is formed.

The cylindrical pipe body bracket is made of high-quality PCL material.

The tubular three-dimensional cell degradable culture bracket provided by the embodiment of the utility model has a simple structure, the cell three-dimensional tubular bracket uses the degradable PCL material as a matrix, cells can grow and expand on the bracket with holes to form tubular cell tissue substances, and the characteristics of human body internal tubular tissues can be better simulated.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

It will be readily understood by those skilled in the art that the present utility model, including any combination of parts described in the summary and detailed description of the utility model above and shown in the drawings, is limited in scope and does not constitute a complete description of the various aspects of these combinations for the sake of brevity. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Although embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives, and variations may be made in the above embodiments by those skilled in the art without departing from the spirit and principles of the utility model. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. The tubular three-dimensional cell degradable culture support is characterized by comprising a cylindrical tube support, wherein a plurality of irregularly-arranged circular grooves are formed in the outer surface of the cylindrical tube support, and a plurality of irregularly-arranged circular grooves are formed in the inner surface of the cylindrical tube support.

2. The tubular three-dimensional cell degradable culture support according to claim 1, wherein the cylindrical tube body support is made of polycaprolactone material.

3. The tubular three-dimensional cell degradable culture support according to claim 1, wherein the thickness of the wall of the cylindrical tube body support is 1mm.

4. The tubular three-dimensional cell degradable culture support according to claim 1, wherein the cylindrical tube support has a cylindrical tubular structure.