CN214252315U - A mould that is used for western blotting electrophoresis to change membrane system to glue - Google Patents
A mould that is used for western blotting electrophoresis to change membrane system to glue Download PDFInfo
- Publication number
- CN214252315U CN214252315U CN202120074988.1U CN202120074988U CN214252315U CN 214252315 U CN214252315 U CN 214252315U CN 202120074988 U CN202120074988 U CN 202120074988U CN 214252315 U CN214252315 U CN 214252315U
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- CN
- China
- Prior art keywords
- cover plate
- western blotting
- gel
- mold
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000001262 western blot Methods 0.000 title claims abstract description 20
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 17
- 239000003292 glue Substances 0.000 title claims description 16
- 239000012528 membrane Substances 0.000 title abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 5
- 239000000758 substrate Substances 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims 2
- 230000006378 damage Effects 0.000 abstract description 3
- 238000010030 laminating Methods 0.000 description 3
- 239000002033 PVDF binder Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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Abstract
The utility model discloses a mould for making gel by electrophoresis film transfer of western blotting method, which comprises a base plate and a first cover plate, wherein the base plate is detachably connected with the first cover plate, three edges of the base plate are provided with grooves, the first cover plate is embedded into the grooves and is tightly connected with the base plate, and the base plate is connected with the first cover plate to form a space for accommodating gel; the problem of bottom weeping can be stopped when preparing the gel, avoids the weeping to lead to reconfiguring solution or make the gel again, greatly improves the reliability of mould, can avoid appearing the bubble moreover, changes the membrane from preparing the gel, all goes on in this mould, makes the improvement in the aspect of the seal when preparing the gel, and system is glued efficiently and improves, convenient operation, is difficult for causing the destruction to the gel when changeing the membrane operation.
Description
Technical Field
The utility model belongs to the technical field of molecular biology, biochemistry and immunology survey, concretely relates to a mould that is used for western blot electrophoresis to change membrane system to glue.
Background
Western blotting (Western Blot) is a commonly used experimental method in molecular biology, biochemistry and immunogenetics, in which a gel electrophoresis-treated cell or tissue sample is stained with a specific antibody, and the treatment of the cell or tissue sample is a critical step. The conventional WB experiments also have some problems in terms of operation and efficiency, such as: leakage can be caused by improper operation when the gel is prepared; bubbles can appear when the two glass plates are not tightly jointed; the gel is required to be transferred to a film transfer device subsequently during film transfer, the gel can be damaged in the process, and the process is complicated.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model provides a mould that is used for western blot electrophoresis to change membrane system to glue carries out in this device from preparation gel to changeing the membrane, makes the improvement in the aspect of the seal when preparing the gel, and system glue efficiency improves, and convenient operation is difficult for causing the destruction to the gel.
In order to achieve the above purpose, the utility model adopts the technical scheme that: a mould for electrophoresis film transfer and glue preparation by a western blot method comprises a base plate, a first cover plate and a second cover plate, wherein the base plate is detachably connected with the first cover plate, the base plate, the first cover plate and the second cover plate are rectangular plates, strip-shaped convex parts are arranged on three edges of the base plate, grooves are formed in the inner sides of the strip-shaped convex parts, the first cover plate is embedded into the grooves and tightly connected with the base plate, and the base plate is connected with the first cover plate to form a space for containing gel; the length and width of the second cover plate are equal to those of the area surrounded by the strip-shaped convex parts, and the second cover plate can be embedded into the area surrounded by the strip-shaped convex parts.
The thickness of the space is 1.5-2.0 mm;
the recess department is provided with the elasticity stuff, fills in the clearance of recess with the stuff, improves base plate and first apron laminating degree, and the space thickness that forms between base plate and the first apron is more close to the theoretical value.
The shape of the groove is dovetail, U-shaped or U-shaped.
The base plate and the first cover plate are provided with strip-shaped clamps at two sides.
The base plate and the first cover plate are made of colorless transparent organic glass.
The base plate and the first cover plate are also provided with transverse and longitudinal scale marks.
The first cover plate is provided with a handle.
The thickness of the second cover plate is not less than that of the first cover plate.
Compared with the prior art, the utility model discloses following beneficial effect has at least: the problem of bottom weeping can be stopped when preparing the gel, avoids the weeping to lead to reconfiguring solution or make the gel again, greatly improves the reliability of mould, can avoid appearing the bubble moreover, changes the membrane from preparing the gel, all goes on in this mould, makes the improvement in the aspect of the seal when preparing the gel, and system is glued efficiently and improves, convenient operation, is difficult for causing the destruction to the gel when changeing the membrane operation.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic sectional view of the structure A-A shown in FIG. 1.
FIG. 3 is a schematic cross-sectional view of the structure B-B shown in FIG. 1.
Fig. 4 is a front view of the first cover plate.
Fig. 5 is a schematic side view of the first cover plate.
Fig. 6 is a front view of the second cover plate.
Fig. 7 is a bottom view of the second cover plate.
In the attached drawing, 1-a substrate, 2-a first cover plate, 3-a groove, 4-a strip-shaped bulge, 5-a second cover plate and 6-a positioning column.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1, fig. 2, fig. 3 and fig. 4, a two-in-one gel-making mold for Western Blot (Western Blot) electrophoresis and film transfer comprises a substrate 1, a first cover plate 2 and a second cover plate 5, wherein the substrate 1 and the first cover plate 2 are detachably connected, and the thickness of the space is 1.5-2.0 mm; grooves are formed in three sides of the substrate 1, the first cover plate 2 is embedded into the grooves and tightly connected with the substrate 1, and the substrate 1 and the first cover plate 2 are connected to form a gel accommodating space; the length and width of the second cover plate 5 are equal to the length and width of the area surrounded by the strip-shaped convex parts 4.
Referring to fig. 1 and 5, the base plate 1, the first cover plate 2 and the second cover plate 5 are all rectangular plates, three edges of the base plate 1 are all provided with grooves 3, and the inner sides of the strip-shaped protruding parts 4 are provided with grooves; the thickness of the second cover 5 is not less than the thickness of the first cover 2.
The groove is provided with the elasticity stuff, fills in the clearance of recess with the stuff, improves base plate 1 and the laminating degree of first apron 2, because the elasticity stuff is thinner, the recess can be put into completely to base plate 1 and the laminating back strip arch of first apron 2, and both sides are inseparabler after fixing with the bar clamp, use no gap between the two boards at every turn.
Referring to fig. 6 and 7, as an alternative embodiment, a positioning column 6 is disposed at an edge of the second cover plate 5, a positioning hole is formed in a position of the strip-shaped protruding portion 4 corresponding to the positioning column 6, and the positioning column 6 is in transition fit with the positioning hole when the second cover plate is in use.
The shape of the groove is dovetail, U-shaped or U-shaped.
The base plate 1 and the first cover plate 2 are provided with clips around the circumference.
The base plate 1 and the first cover plate 2 are made of colorless transparent organic glass.
The first cover plate 2 is provided with a handle, and the first cover plate 2 is also provided with transverse and longitudinal scale marks.
Example (b):
the blotting filter paper is fixed on the surface of the substrate 1, so that the adhesion degree of glue and the substrate is increased, the first cover plate 2 can be conveniently taken down and added with a PVDF film, and the blotting filter paper in the traditional Western Blot electrophoresis is reserved on the other hand, thereby being beneficial to generating uniform buffer liquid flow, shortening the film transferring time and realizing the continuous and efficient transfer. The top seals filter paper to wrap partly filter paper downwards, press from both sides filter paper in the centre, guaranteed that filter paper is not more than the gel, prevent the short circuit, the part that the upper strata was glued can directly be regarded as to the part that moreover was many out, and the application of sample is convenient for to transparent design.
When transferring the film, the first cover plate 2 is removed, the PVDF film is placed on the first cover plate, and then the second cover plate 5 is mounted and clamped, and the film transfer is started.
Claims (9)
1. The mold for electrophoresis film transfer and glue preparation by the western blotting method is characterized by comprising a substrate (1), a first cover plate (2) and a second cover plate (5), wherein the substrate (1) is detachably connected with the first cover plate (2), the substrate (1), the first cover plate (2) and the second cover plate (5) are rectangular plates, strip-shaped protruding parts (4) are arranged on three sides of the substrate (1), grooves (3) are formed in the inner sides of the strip-shaped protruding parts (4), the first cover plate (2) is embedded into the grooves and tightly connected with the substrate (1), and the substrate (1) and the first cover plate (2) are connected to form a space for containing gel; the length and the width of the second cover plate (5) are equal to those of the area surrounded by the strip-shaped convex parts (4), and the second cover plate (5) can be embedded into the area surrounded by the strip-shaped convex parts (4).
2. The mold for western blotting electrophoresis transfer film glue according to claim 1, wherein the thickness of the gel-containing space formed after the base plate (1) and the first cover plate (2) are tightly connected is 1.5-2.0 mm.
3. The mold for western blotting electrophoresis transfer film glue making according to claim 1, wherein an elastic plug strip is disposed at the groove.
4. The mold for western blotting electrophoresis transfer film glue according to claim 1, wherein the shape of said groove is dovetail, U-shape or "U" shape.
5. The mold for western blotting electrophoresis transfer film glue making according to claim 1, wherein the base plate (1) and the first cover plate (2) are provided with a strip-shaped clamp at both sides.
6. The mold for western blotting electrophoresis film transfer and glue making according to claim 1, wherein the material of the substrate (1) and the material of the first cover plate (2) are both colorless transparent organic glass.
7. The mold for western blotting electrophoresis transfer film glue making according to claim 1, wherein the base plate (1) and the first cover plate (2) are further provided with transverse and longitudinal scale marks.
8. The mold for western blotting electrophoresis transfer film glue making according to claim 1, wherein the first cover plate (2) is provided with a handle.
9. The mold for western blotting electrophoresis transfer film glue according to claim 1, wherein the thickness of the second cover plate (5) is not less than the thickness of the first cover plate (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120074988.1U CN214252315U (en) | 2021-01-12 | 2021-01-12 | A mould that is used for western blotting electrophoresis to change membrane system to glue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120074988.1U CN214252315U (en) | 2021-01-12 | 2021-01-12 | A mould that is used for western blotting electrophoresis to change membrane system to glue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214252315U true CN214252315U (en) | 2021-09-21 |
Family
ID=77725513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120074988.1U Expired - Fee Related CN214252315U (en) | 2021-01-12 | 2021-01-12 | A mould that is used for western blotting electrophoresis to change membrane system to glue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214252315U (en) |
-
2021
- 2021-01-12 CN CN202120074988.1U patent/CN214252315U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210921 |