CN214224556U

CN214224556U - Individual collecting device for schistosoma japonicum cercaria specimen

Info

Publication number: CN214224556U
Application number: CN202120046601.1U
Authority: CN
Inventors: 吕大兵; 张洁英; 顾漫漫; 孙梦涛; 余秋夫
Original assignee: Suzhou University
Current assignee: Suzhou University
Priority date: 2021-01-08
Filing date: 2021-01-08
Publication date: 2021-09-17
Anticipated expiration: 2031-01-08

Abstract

The application provides an individual collection device of schistosoma japonicum cercaria specimen, which comprises: a handle body; the cercaria ring is used for contacting and sinking into a water body so as to catch individual Japanese blood cercaria floating on the water surface and then adhere the Japanese blood cercaria to a target object, and the cercaria ring is provided with a gap capable of being opened and closed; the connecting rod is used for connecting the handheld handle body 1 with the cercaria ring; wherein, the cercaria ring and a horizontal plane have an included angle, and the horizontal plane is vertical to the connecting rod. The water membrane formed by the cercaria ring on the water surface is convenient for collecting the schistosoma japonicum cercaria specimens one by one, and the opening and closing notch is arranged on the cercaria ring to facilitate the rupture of the water membrane, thus ensuring that the cercaria can be successfully adhered to the infected body or the preservation card along with the water membrane.

Description

Individual collecting device for schistosoma japonicum cercaria specimen

Technical Field

The application relates to the field of biological equipment, in particular to an individual collecting device for a schistosoma japonicum cercaria specimen.

Background

At present, in experimental researches such as schistosomiasis vaccine development, drug tests and the like, a cercaria adhesion cover glass infection method is commonly used when a mouse model for schistosomiasis infection is established. However, in the cover glass adhesion cercaria infection method, because the cercaria has strong adhesion, the cercaria can be excessively adsorbed on the surface, and meanwhile, the small body (the body length is 100-150 μm) of the cercaria is easy to have counting errors, which is not beneficial to accurate quantification and directly influences result evaluation; moreover, the method can not collect and store cercaria specimens one by one, and is difficult to meet the requirements of schistosome genetics research.

Accordingly, there is a need for improvements in the art that overcome the deficiencies in the prior art.

Content of application

The application aims to provide the individual collecting device for the schistosoma japonicum cercaria specimen, which can efficiently and gradually collect the schistosoma japonicum cercaria.

In order to achieve the purpose, the application provides the following technical scheme: an individual collecting device for a schistosoma japonicum cercaria specimen comprises:

a handle body;

the cercaria ring is used for contacting and sinking into a water body so as to catch individual Japanese blood cercaria floating on the water surface and then adhere the Japanese blood cercaria to a target object, and the cercaria ring is provided with a gap capable of being opened and closed;

the connecting rod is used for connecting the handheld handle body with the cercaria ring;

wherein an included angle is formed between the cercaria ring and the horizontal plane, the included angle is set to be 3-8 degrees, and the horizontal plane is perpendicular to the connecting rod.

Furthermore, a mounting hole is formed in one end of the handheld handle body, and one end of the connecting piece penetrates through the mounting hole to be connected with the handheld handle body.

Furthermore, a thread is arranged at one end of the connecting piece penetrating through the mounting hole, the individual collecting device for the schistosoma japonicum cercaria specimen further comprises a nut matched with the connecting piece, and the connecting piece is fixedly connected with the handheld handle body through the nut.

Further, the diameter of the cercaria ring is set to be 1.0mm-2.0 mm.

Further, the material of the handheld handle body is polyester fiber.

Further, the material of the connecting rod and the cercaria ring is metal.

The beneficial effect of this application lies in: water films can be formed on the water surface through the cercaria rings to collect cercaria specimens one by one, and the cercaria rings are provided with openings which can be opened and closed to facilitate the breaking of the water films, so that the schistosoma japonicum cercaria can be successfully adhered to an infected body or a preservation card along with the water films.

The foregoing description is only an overview of the technical solutions of the present application, and in order to make the technical solutions of the present application more clear and clear, and to implement the technical solutions according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present application and the accompanying drawings.

Drawings

Fig. 1 is a schematic structural diagram of the present application.

Fig. 2 is a schematic view of the opening of the gap in fig. 1.

Detailed Description

The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

In addition, the technical features mentioned in the different embodiments of the present application described below may be combined with each other as long as they do not conflict with each other. In the description of the present application, the axis direction coincides with the height direction.

Referring to fig. 1 and 2, the individual collecting device 100 for schistosoma japonicum cercaria specimen in the present application comprises a handheld handle body 1 for holding by hand, a cercaria ring 2 for contacting and sinking into a water body to catch the schistosoma japonicum cercaria floating on the water surface and then adhering the schistosoma japonicum cercaria to a target object (such as an infection surface (such as mouse skin) or a preservation card), and a connecting rod 3 for connecting the handheld handle body 1 and the cercaria ring 2.

Wherein, have certain distance with cercaria ring 2 when being convenient for draw and guaranteeing in the laboratory staff to hold handheld handle body 1, set up handheld handle body 1 into diameter 2mm, length 8.5 cm's straight-bar, avoid the staff to touch cercaria ring 2 and take place the infection danger, simultaneously, for hardness and the corrosion resistance of guaranteeing handheld handle body 1, set up the material of handheld handle body 1 into polyester fiber.

In this embodiment, in order to facilitate the installation and the detachment between the handheld handle body 1 and the connecting piece, a mounting hole 11 is formed in one end of the handheld handle body 1 close to the connecting piece, one end of the connecting piece penetrates through the mounting hole 11 to be connected with the handheld handle body 1, a thread is formed in one end of the connecting piece penetrating through the mounting hole 11, the schistosoma japonicum cercaria specimen individual collecting device 100 further comprises a nut 4 matched with the connecting piece, and the connecting piece is fixedly connected with the handheld handle body 1 through the nut 4. Specifically, the connecting rod 3 includes a rod main body 31 and a connecting portion 32 connected to the rod main body 31 and disposed near one end of the handheld handle body 1, wherein the diameter of the rod main body 31 is greater than the mounting hole 11, the diameter of the connecting portion 32 is smaller than the mounting hole 11, the connecting portion 32 penetrates through the mounting hole 11 and enables one end of the rod main body 31 to abut against the handheld handle body 1, a thread is disposed on an outer wall of the connecting portion 32, a nut 4 is disposed at one end of the mounting hole 11 away from the rod main body 31, and the handheld handle body 1 and the connecting rod 3 are gradually tightened to enable the handheld handle body 1 and the connecting rod 3 to be tightly connected through cooperation of the nut 4 and the connecting portion 32.

The cercaria ring 2 is arranged in a circular ring shape with a diameter of 1.0mm-2.0mm, preferably, the cercaria ring 2 is arranged in a diameter of 1.5mm, so that when the cercaria ring 2 is immersed in the liquid containing the schistosoma japonicum cercaria, a water film with the schistosoma japonicum cercaria is formed on the cercaria ring 2, extracting the fasciola japonica cercaria with water film, pressing the cercaria ring 2 on the target object, pressing with slight force to deform the cercaria ring 2, breaking the water film, extracting the fasciola japonica cercaria to the target object, in order to form a water film on the cercaria ring 2, an included angle is arranged between the cercaria ring 2 and the horizontal plane, wherein, the horizontal plane is perpendicular to the connecting rod 3, and the contained angle sets up to 3 ~ 8, preferably, and the contained angle sets up to 5, is convenient for cercaria ring 2 to have certain gradient in soaking liquid, makes to form the water film and be difficult for breaking after cercaria ring 2 soaks liquid. In order to ensure that the water film is easy to break after the cercaria ring 2 is flatly pressed on a target object, the cercaria ring 2 is provided with a notch 21 which can be opened and closed, at the moment, when the cercaria ring 2 is immersed in liquid to fish for the schistosoma japonicum cercaria, the notch 21 is closed, and the water film is convenient to form; when the schistosoma japonicum cercaria is extracted to the target object, the opening 21 is opened, the water film automatically breaks because of the opening 21, and the schistosoma japonicum cercaria is extracted to the target object. In this embodiment, in order to facilitate opening and closing of the notch 21, the cercaria ring 2 is set to have certain toughness, so that when the cercaria ring 2 is pressed, the cercaria ring 2 deforms to open the closed notch 21 (see fig. 2), at this time, the cercaria ring 2 is made of metal, such as iron, copper and the like, the manufacturing is simple, the material is easy to obtain, in order to facilitate connection between the cercaria ring 2 and the connecting rod 3, the connecting rod 3 is made of metal, preferably, the cercaria ring 2 and the connecting rod 3 are made of the same material, so that the cercaria ring 2 and the connecting rod 3 are integrally formed, the connecting rod 3 is tangent to the peripheral wall of the cercaria ring 2, and the connecting rod 3 and the cercaria ring 2 are burnt to achieve sterilization.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. An individual collecting device for a schistosoma japonicum cercaria specimen is characterized by comprising:

a handle body;

2. The device for collecting individual schistosoma japonicum cercaria specimen of claim 1, wherein an installation hole is formed at one end of said handle body, and one end of said connecting rod passes through said installation hole and connects with said handle body.

3. The Schistosoma japonicum cercaria specimen individual collecting device of claim 2, wherein one end of said connecting rod passing through said mounting hole is provided with a thread, said Schistosoma japonicum cercaria specimen individual collecting device further comprises a nut matching with said connecting rod, and said connecting rod is fastened with said handheld handle body 1 through a nut.

4. The device for collecting individual specimens of Schistosoma japonicum cercaria of claim 1, wherein the diameter of said cercaria ring is set to be 1.0mm-2.0 mm.

5. The Schistosoma japonicum cercaria specimen individual collecting device of claim 1 wherein the material of said handle is polyester fiber.

6. The device for collecting individual samples of Schistosoma japonicum cercaria of claim 1, wherein the material of said connecting rod and said cercaria ring is metal.