CN213986194U

CN213986194U - Fluorescence detection device

Info

Publication number: CN213986194U
Application number: CN202022915286.XU
Authority: CN
Inventors: 李维; 周志图
Original assignee: Bioteke Corp
Current assignee: Bioteke Corp
Priority date: 2020-12-08
Filing date: 2020-12-08
Publication date: 2021-08-17
Anticipated expiration: 2030-12-08

Abstract

The utility model discloses a fluorescence detection device, include: the light source device comprises a shell, a light emitting channel and a light receiving channel are arranged in the shell, a point light source and two plano-convex lenses with opposite convex surfaces and arranged at intervals are arranged in the light emitting channel from inside to outside, and light emitted by the point light source passes through the two plano-convex lenses to form an emitting light path; the receiving light channel is provided with a photodiode and two plano-convex lenses with opposite convex surfaces and arranged at intervals from inside to outside, reflected light passes through the two plano-convex lenses to the photodiode to form a receiving light path, photoelectric conversion is realized by the photodiode, and light filters are arranged in the transmitting light channel and the receiving light channel. The device collimates and refocuses the light path through two plano-convex lenses arranged at intervals, thereby greatly improving the utilization rate of light rays of a light source and the utilization rate of received light; through the leak protection light design, not only prevent inside outside parasitic light infiltration entering fluorescence device, still avoid emitting light and receive the light interpenetration interference, improved the detection precision of device.

Description

Fluorescence detection device

Technical Field

The utility model belongs to the technical field of fluorescence detection and specifically relates to a fluorescence detection device is related to.

Background

The fluorescence detection is a natural luminescence reaction, and can detect human cells, bacteria, mould and food residues by reacting luciferase with ATP. The illuminance is measured by a dedicated device and is represented in digital form. Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference or external reference method by measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in a DNA amplification reaction. Real-time PCR is a Real-time detection of PCR progress by fluorescence signals during PCR amplification.

In the fluorescence detection device in the prior art, the light path is generally designed to be that the light source is directly projected on a sample to be detected or projected on a reflector, then the detector receives a feedback light source signal of the sample, so that the operation is carried out, the power utilization rate of the light source is low, and the detection of the feedback light signal can be weakened.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: in order to overcome the not enough of background art, the utility model discloses a fluorescence detection device is through carrying out the collimation with some light source light path and refocusing in order to improve the light source utilization ratio.

The technical scheme is as follows: fluorescence detection device, include: the device comprises a shell, a light emitting channel and a light receiving channel are arranged in the shell, a point light source and two plano-convex lenses with opposite convex surfaces and arranged at intervals are arranged in the light emitting channel from inside to outside, and emitted light of the point light source passes through the two plano-convex lenses to be straightened and then focused to form an emitting light path; the receiving light channel is provided with a photodiode and two plano-convex lenses with opposite convex surfaces and arranged at intervals from inside to outside, reflected light passes through the two plano-convex lenses to be straightly focused on the photodiode to form a receiving light path, photoelectric conversion is realized by the photodiode, and light filters are arranged in the transmitting light channel and the receiving light channel.

The fluorescence detection device firstly collimates and then focuses the emitted light of the point light source by two plano-convex lenses, selects a required radiation waveband through an optical filter and projects the waveband onto a sample to be detected; the received light passes through two plano-convex lenses and a filter by a receiving light channel according to the same principle, and finally, the photoelectric conversion is realized by a photodiode.

Further, the shell is formed by splicing the first shell and the second shell and is fixed through screws, and the spliced positions of the two shells are spliced by adopting stepped folding surfaces to form a light leakage prevention structure. Not only prevents external stray light from penetrating into the fluorescent device, but also avoids the mutual penetration interference between the emitted light and the received light.

In order to further deliver a shading effect and facilitate the mounting and fixing of the device, the plano-convex lens and the optical filter are both fixed in the housing by an annular clamping member.

Furthermore, the point light source and the photodiode are fixed on the circuit board and extend out of the shell.

In the fluorescence detection device, the emission light channel and the receiving light channel are arranged side by side in parallel.

The optical filter is arranged between the two plano-convex lenses of the optical path, and the required radiation wave band is selected in the stage of the straight light.

Has the advantages that: compared with the prior art, the utility model has the advantages that: firstly, the device collimates and refocuses the light path through two plano-convex lenses arranged at intervals, thereby greatly improving the utilization rate of light rays of a light source and the utilization rate of received light; secondly, through carrying out the leak protection light design to the device casing, not only prevent that outside parasitic light from permeating inside getting into fluorescence device, can also avoid emitting light and receiving light interpenetration interference, improved the detection precision of device.

Drawings

FIG. 1 is a schematic view of the internal structure of the present invention;

FIG. 2 is a schematic view of the structure of the housing of the present invention;

FIG. 3 is a structural view of the shell splicing part of the present invention;

fig. 4 is a light path simulation diagram of the present invention.

Detailed Description

The technical solution of the present invention will be further explained with reference to the accompanying drawings and examples.

The fluorescence detection device shown in fig. 1 includes: the light emitting device comprises a shell 1, wherein a light emitting channel 2 and a light receiving channel 3 are arranged in the shell 1, the light emitting channel 2 and the light receiving channel 3 are arranged in parallel side by side, and the shell is led out from the interior of the shell 1. The inner side end of the emission light channel 2 is provided with a point light source 4, namely an LED light source, the inner side end of the receiving light channel 3 is provided with a photodiode 6, the point light source 4 emits light to form an emission light path in the emission light channel 2 to be projected to a sample to be measured, the feedback light of the sample to be measured receives light, the receiving light channel 3 forms a receiving light path, and the light is emitted to the photodiode 6 to be subjected to photoelectric conversion.

Because there is the divergence phenomenon in the transmission light of pointolite 4, be equipped with two planoconvex lens 5 respectively in transmission light passageway 2 and receipt light channel 3, two planoconvex lens 5 convex surfaces in the same passageway are relative, the interval sets up, set up an optical filter 7 simultaneously between two planoconvex lens 5, light realizes the light collimation through first planoconvex lens (being collimating lens) in the passageway, select required radiation wave band through the optical filter again, realize the straight light focus through second planoconvex lens (being focusing lens) at last, thereby improve the utilization ratio of light source.

As shown in fig. 2, the housing 1 is formed by splicing a first housing 101 and a second housing 102 and is fixed by screws, the point light source 4 and the photodiode 6 are both fixed on the circuit board and extend out of the housing 1, during installation, the plano-convex lens 5 and the optical filter 7 are fixed in a mounting groove pre-formed in the first housing 101 through respective annular clamping members 8, then the second housing 102 is covered, and the screws are screwed up to realize fixation.

As shown in fig. 3, in order to avoid external stray light from penetrating into the interior of the fluorescent device and mutual penetrating interference between the emitted light and the received light, a stepped folding surface is adopted at the joint of the two shells to form a light leakage prevention structure.

As shown in fig. 4, light paths are simulated by using ZEMAX optical software, and light rays are collimated and focused by sequentially passing through a plano-convex lens in combination with fig. 1, and the data result shows that the utilization rate of a light source reaches about 80%.

Claims

1. A fluorescence detection device, comprising: the light source device comprises a shell (1), wherein a light emitting channel (2) and a light receiving channel (3) are arranged in the shell (1), a point light source (4) and two plano-convex lenses (5) with opposite convex surfaces and arranged at intervals are arranged in the light emitting channel (2) from inside to outside, and emitted light of the point light source (4) passes through the two plano-convex lenses (5) to be focused to form an emitting light path; receive light channel (3) from interior to exterior and be equipped with photodiode (6) and two convex surfaces relative, plano-convex lens (5) that the interval set up, the reverberation passes two plano-convex lens (5) and carries out straight refocusing to photodiode (6) and forms and receive the light path to realize photoelectric conversion by photodiode (6), all be equipped with light filter (7) in transmitting light channel (2) and receiving light channel (3).

2. The fluorescence detection device according to claim 1, characterized in that: the shell (1) is formed by splicing a first shell (101) and a second shell (102) and is fixed through screws, and the spliced part of the two shells adopts a stepped folding surface to splice to form a light leakage prevention structure.

3. The fluorescence detection device according to claim 1, characterized in that: the plano-convex lens (5) and the optical filter (7) are fixed in the shell (1) through an annular clamping piece (8).

4. The fluorescence detection device according to claim 1, characterized in that: the point light source (4) and the photodiode (6) are fixed on the circuit board and extend out of the shell (1).

5. The fluorescence detection device according to claim 1, characterized in that: the light emitting channel (2) and the light receiving channel (3) are arranged in parallel side by side.

6. The fluorescence detection device according to claim 1, characterized in that: the optical filter (7) is arranged between the two plano-convex lenses (5) on the optical path.