CN213977733U

CN213977733U - Cell suspension culture aerating device for enzyme-linked immunosorbent assay experiments

Info

Publication number: CN213977733U
Application number: CN202022611442.3U
Authority: CN
Inventors: 曾珍; 韩芳; 陈剑; 韦海燕
Original assignee: Jiangsu Maisha Industrial Co ltd
Current assignee: Jiangsu Maisha Industrial Co ltd
Priority date: 2020-11-12
Filing date: 2020-11-12
Publication date: 2021-08-17
Anticipated expiration: 2030-11-12

Abstract

The utility model discloses a cell suspension culture aerating device for enzyme-linked immunosorbent assay experiments, which comprises a tank body and an aerating tank,a water pump is arranged in the tank body, a tank cover is arranged at the top of the tank body, an air pressure gauge and an end cover are respectively arranged on the tank cover, and the air pressure gauge is used for measuring the air pressure in the tank body; an air pump is arranged in the inflation tank and is communicated with the tank body through a gas pipe; a rotating rod penetrates through the inflation box along the central axis, one end of the rotating rod is connected with an output shaft of a motor, fan blades are sleeved on the rotating rod, the motor drives the rotating rod to rotate, so that the gas in the inflation box is uniformly mixed, and then the gas is sent into the tank body through a gas pipe by a gas pump; the utility model discloses an experimental cell suspension culture aerating device of ELISA adsorption method can aerify the operation of gassing simultaneously, satisfies the experiment requirement, can make the CO who fills simultaneously₂And the air is uniformly mixed, so that the experimental effect is improved.

Description

Cell suspension culture aerating device for enzyme-linked immunosorbent assay experiments

Technical Field

The utility model belongs to the technical field of cell suspension culture, in particular to a cell suspension culture aerating device for ELISA (enzyme linked immunosorbent assay) experiments.

Background

Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells. Certain anchorage-dependent cells can also be cultured using this method, after adaptation and selection. It is relatively simple to increase the scale of suspension culture, as long as the volume is increased. The depth is more than 5mm, the culture medium is stirred for more than 10cm, and CO is deeply introduced₂And air to ensure adequate gas exchange.

In the production of biomedical reagents, particularly chicken immunoglobulin G (IgG) reagents, samples such as chicken serum, homogenate and the like need to be cultured to obtain tissue specimens, and suspension culture is an important way for cell culture. In the initial stage of cell suspension culture, before large-scale production fermentation culture, laboratory conditions are usually preliminarily studied, and CO is periodically filled into the culture flask in the process₂And air to balance pH value in the culture bottle, discharge waste gas of cell metabolism and provide oxygen for cell respiration, and sealing the culture bottle to suspend fine particlesThe cells continue to grow. Most of the existing cell suspension culture aerating devices can not perform aeration and deflation operations simultaneously, so that the experimental effect is influenced.

Therefore, the present inventors have made research and improvement on this, and have come to the present invention.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an experimental aerating device of cultivateing of cell suspension of ELISA adsorption method can aerify the operation of gassing simultaneously, satisfies the experiment requirement, can make the CO who fills simultaneously₂And the air is uniformly mixed, so that the experimental effect is improved.

In order to achieve the above purpose, the solution of the present invention is:

a cell suspension culture aerating device for an enzyme-linked immunosorbent assay experiment comprises a tank body and an aerating tank, wherein a water pump is arranged in the tank body, a tank cover is arranged at the top of the tank body, and is respectively provided with a barometer and an end cover, and the barometer is used for measuring the air pressure in the tank body;

an air pump is arranged in the inflation tank and is communicated with the tank body through a gas pipe; the dwang has been run through along the axis in the inflation box, and the one end of dwang is equipped with the flabellum with the output shaft of motor on this dwang, and motor drive dwang rotates, makes the gaseous misce bene in the inflation box, passes through the gas-supply pipe by the air pump again and sends into a jar body.

The inflation box is communicated with the inflation bottle through an inflation tube, and a switch valve is arranged on the inflation tube.

A plurality of rows of cell placing racks are arranged in the tank body from top to bottom in parallel.

The top of the above-mentioned jar body and the bottom of cover, the junction of the two all is formed with the flange, and the flange runs through has a plurality of eyebolts, and jar body and cover are fixed each other with the help of eyebolt.

The joint of the tank body and the tank cover is provided with a sealing gasket.

An access door is arranged on the side surface of the tank body.

The side of the inflation box is provided with a heat dissipation plate.

The side surface of the tank body is also provided with an air outlet communicated with the inside and the outside.

The fan blades spirally surround the periphery of the rotating rod.

After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:

(1) the cell suspension culture aerating device for ELISA experiments is convenient to control the pressure of cell culture by installing a barometer; the pH value of the cultured cells can be conveniently adjusted by installing a water pump; ensuring that the experimental process meets the required conditions;

(2) the cell suspension culture aerating device for ELISA experiments enables CO to be filled in through installing fan blades₂The air and the air are uniformly mixed, so that the experimental effect is improved;

(3) according to the cell suspension culture aerating device for the ELISA experiment, bacteria in the external environment are prevented from entering the tank body to pollute cells by installing the end cover; and a sealing gasket is arranged between the tank cover and the tank body, so that the sealing property of the culture tank is improved, and the possibility of cell pollution is effectively reduced;

(4) this kind of cell suspension culture aerating device for ELISA experiments through installation eye bolt, is convenient for dismantle and install the cover.

The device simple structure is reasonable, and convenient to use has solved effectively that the tolerance and the time error of inflating gas are great, and the investigation of experimental condition is difficult to control, current aerating device inconvenient operation and the inhomogeneous problem of gas mixture have higher practical value.

Drawings

Fig. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic front sectional view of the present invention;

FIG. 3 is an enlarged view of the structure at A in FIG. 2;

fig. 4 is a schematic structural diagram of the on-off valve of the present invention.

Reference numerals:

1. a tank body; 2. an air outlet; 3. an access door;

4. an eye bolt; 5. a can lid; 6. an end cap;

7. a gas delivery pipe; 8. an air-filled tank; 9. a heat dissipation plate;

10. a controller; 11. an on-off valve; 12. inflating the bottle;

13. a barometer; 14. placing a cell rack; 15. rotating the rod;

16. a storage battery; 17. a water pump; 18. a fan blade;

19. an electric motor; 20. an air pump; 21. a valve stem;

22. a valve cover; 23. and an inflation tube.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

As shown in fig. 1 to 2, the utility model provides a cell suspension culture aerating device for enzyme-linked immunosorbent assay experiments, which comprises a tank body 1, wherein a storage battery 16 is fixedly arranged at the bottom side inside the tank body 1, and a water pump 17 is fixedly arranged at one side of the storage battery 16; the top fixed mounting of the jar body 1 has a cover 5, the top fixed mounting of cover 5 has

barometer

13, 13 one side fixed mounting of barometer has

end cover

6, 6 one side of end cover is provided with gas-supply pipe 7, 7 one end fixed mounting of gas-supply pipe has plenum box 8, the inside bottom side fixed mounting of plenum box 8 has

air pump

20, 20 top one side fixed mounting of air pump has motor 19, 8 inside running through of plenum box has dwang 15, and the one end of dwang 15 passes through the shaft coupling transmission with motor 19's output shaft and is connected, 15 covers of dwang are equipped with flabellum 18.

As a preferred embodiment of the present invention, as shown in fig. 1, an inflation tube 23 is fixedly installed on one side of the inflation box 8, the cover of the inflation tube 23 is provided with a switch valve 11, and the switch valve 11 is connected with the inflation tube 23, a valve cover 22 is fixedly installed on the top of the switch valve 11, and can be matched with the valve rod 21 fixedly installed on the top of the valve cover 22, and an inflation bottle 12 is installed on one end of the inflation tube 23, so as to conveniently stop inflation and adjust air pressure.

As a preferred embodiment of the present invention, as shown in FIG. 2, the cell placing frame 14 is disposed inside the tank body 1, and the cell placing frame 14 is arranged in a matrix arrangement, thereby improving the space utilization efficiency.

As a preferred embodiment of the present invention, as shown in FIG. 1 and FIG. 3, the top of the tank body 1 and the bottom of the tank cover 5 are both provided with flanges, a plurality of eye bolts 4 are installed in the flanges in a penetrating manner, and the joint of the tank body 1 and the tank cover 5 is provided with a sealing gasket, so that the sealing performance of the culture tank is improved, and the possibility of cell contamination is effectively reduced.

As a preferred embodiment of the utility model, as shown in FIG. 1, the outside one side of jar body 1 is provided with access door 3, and access door 3 sets up in the one side that is close to battery 16, is convenient for overhaul jar internal structure.

As a preferred embodiment of the present invention, as shown in fig. 1, a heat dissipation plate 9 is fixedly installed on one side of the air inflation box 8, and a controller 10 is fixedly installed on one side of the front surface of the air inflation box 8, so as to facilitate the inflation operation, improve the service time of the inflation device, reduce the working strength of the personnel, and improve the efficiency.

As a preferred embodiment of the utility model, as shown in figure 1, one side outside the tank body 1 is provided with a gas outlet 2, and the gas outlet 2 is fixedly installed on one side of the access door 3 through a bolt, so as to facilitate the discharge of oxygen and the adjustment of the pressure inside the culture tank.

As a preferred embodiment of the present invention, as shown in fig. 2, the fan blade 18 is spirally installed outside the rotating rod 15, and the axis of the rotating rod 15 is aligned with the motor 19, so that the charging gas is uniformly mixed.

The utility model discloses a theory of operation is: this kind of cell suspension culture aerating device for ELISA experiments during operation, open end cover 6, put into the cell culture tank, close end cover 15, set up cultivation parameters such as temperature, humidity, pH valve on operating panel, the culture solution passes through water pump 17 and inserts in the culture tank, air pump 20 supplements CO2 and air to jar 1 inside, and then adjusts the concentration that cultivates the cell needs, starter motor 19 drive dwang 15 rotates, flabellum 18 rotates and carries out intensive mixing to the gas that fills. According to the cell suspension culture aerating device, the switching valve 11 is arranged on the aerating tube 23, so that gas conveying and gas transmission closing are facilitated; the side wall of the tank body 1 is provided with the air outlet 2, so that oxygen can be conveniently discharged and the air pressure in the culture tank can be conveniently adjusted; the pressure of cell culture is conveniently controlled by installing the barometer 13; the water pump 17 is arranged, so that the pH value of the cultured cells can be conveniently adjusted; the lifting eye bolt 4 is arranged, so that the tank cover 6 is convenient to disassemble and assemble, and the sealing gasket is arranged between the tank cover 6 and the tank body 1, so that the sealing property of the culture tank is improved, and the possibility of cell pollution is effectively reduced; the maintenance of the internal structure of the tank body is facilitated by installing the maintenance door 3; by installing the controller 10, the inflation operation is convenient, the working strength of personnel is reduced, and the efficiency is improved; the charging gas is uniformly mixed by installing the fan blades 18; by installing the heat dissipation plate 9, the service time of the inflating device is prolonged; the device simple structure is reasonable, and convenient to use has effectively solved among the cell suspension culture device and has inflated gassy tolerance and time error great, and the groping of experimental condition is difficult to control, aerifys inconvenient operation and gas mixing inhomogeneous problem, has higher practical value.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims

1. A cell suspension culture aerating device for enzyme-linked immunosorbent assay experiments is characterized in that: the tank comprises a tank body and an inflation box, wherein a water pump is arranged in the tank body, a tank cover is arranged at the top of the tank body, an air pressure gauge and an end cover are respectively arranged on the tank cover, and the air pressure gauge is used for measuring the air pressure in the tank body;

2. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: the inflation box is communicated with the inflation bottle through an inflation tube, and a switch valve is arranged on the inflation tube.

3. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: a plurality of rows of cell placing racks are arranged in the tank body from top to bottom in parallel.

4. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: the top of the jar body and the bottom of cover, the junction of the two all is formed with the flange, and the flange runs through has a plurality of eyebolts, and jar body and cover are fixed each other with the help of eyebolt.

5. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: and a sealing gasket is arranged at the joint of the tank body and the tank cover.

6. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: an access door is arranged on the side surface of the tank body.

7. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: and a heat dissipation plate is arranged on the side surface of the inflation box.

8. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: the side surface of the tank body is also provided with an air outlet communicated with the inside and the outside.

9. The cell suspension culture aerating device for ELISA experiments of claim 1 wherein: the fan blades spirally surround the periphery of the rotating rod.