CN213951136U - Purification device for full-automatic nucleic acid extraction instrument - Google Patents

Purification device for full-automatic nucleic acid extraction instrument Download PDF

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Publication number
CN213951136U
CN213951136U CN202022198083.3U CN202022198083U CN213951136U CN 213951136 U CN213951136 U CN 213951136U CN 202022198083 U CN202022198083 U CN 202022198083U CN 213951136 U CN213951136 U CN 213951136U
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China
Prior art keywords
limiting
plate
rod
block
nucleic acid
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Expired - Fee Related
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CN202022198083.3U
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Chinese (zh)
Inventor
张健
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Tianjin Zhongyi Heyuan Medical Technology Co ltd
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Tianjin Zhongyi Heyuan Medical Technology Co ltd
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Priority to CN202022198083.3U priority Critical patent/CN213951136U/en
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Abstract

The utility model provides a purification device for a full-automatic nucleic acid extraction instrument, which is characterized by comprising a stirring sleeve structure, a locking rod, a spring, a limiting rod and a limiting component are arranged, the sliding installation of a mounting plate and a frame is realized through the matching use of a slide block and a slide plate in the application, the locking rod in a locking hole realizes the fixed installation of the slide plate, the slide block and the mounting plate under the action of spring force, the limiting rod in the limiting hole realizes the effective limiting of the position of the locking rod under the action of the spring force, the limiting plate is in direct contact with the locking rod, the limiting plate plays a certain limiting locking role on the limiting rod, the separation of the locking rod and the limiting rod in the mounting process of the mounting plate can be effectively prevented, the phenomenon that the stirring sleeve falls down can not be caused, certain influence can not be caused on the quality of the stirring sleeve, and the stability of the stirring sleeve and the mounting plate can be improved to a certain extent, the operation of changing and disassembling the stirring sleeve and the mounting plate is realized.

Description

Purification device for full-automatic nucleic acid extraction instrument
Technical Field
The utility model relates to a nucleic acid extraction appearance technical field especially relates to full-automatic nucleic acid extraction is purification device for appearance.
Background
In the field of genetic engineering and protein engineering technology research, nucleic acid molecules are main research objects, the separation and linearization of nucleic acid are basic technologies of nucleic acid research, the nucleic acid is a basic unit representing the genetic characteristics of a living body, the nucleic acid detection is advanced biological detection on a molecular level, and compared with the traditional morphological detection, cytology detection, immunology detection and the like, the nucleic acid detection has the remarkable advantages of sensitivity, specificity, no window period and the like, the nucleic acid detection comprises the technologies of qualitative PCR (polymerase chain reaction), molecular hybridization, real-time fluorescence quantitative PCR and the like, and the first key of the nucleic acid detection technologies is to finish extracting the nucleic acid of a biological sample, so that the effective and accurate extraction of a nucleic acid template becomes the premise of subsequent nucleic acid detection;
at present, in the prior art in China, the traditional manual preparation method is mainly adopted for extracting nucleic acid, the manual method for extracting nucleic acid has low efficiency and high cost, the repeatability and the stability are poor, and special large-scale instruments have high use cost and are not suitable for small and medium-sized detection; at present, the extraction of nucleic acid by a magnetic bead extraction method is a better extraction method, a buffer solution needs to be added and small magnetic beads need to be used for extraction in the nucleic acid extraction process, the buffer solution, the small magnetic beads and the cracked nucleic acid are mixed, and the manual shaking mixing effect is difficult to realize a better effect, so that technicians in related fields study a stirring and purifying device of a nucleic acid extractor for a small laboratory;
according to the stirring and purifying device of the nucleic acid extractor in the prior art, through the design of a motor, a base and an extraction vessel, the motor arranged in the extraction vessel is arranged on the base through a rotating shaft of the motor, the extraction vessel integrated with the base is driven by a speed reduction motor to rotate, and nucleic acid extracting solution, buffer solution and small magnetic beads in the extraction vessel are stirred and mixed, so that the small magnetic beads fully adsorb nucleic acid molecules, and the extraction of nucleic acid by a magnetic bead method is realized; through the design of the sliding chute, the sliding rail and the sliding block, the process that the magnetic bar adsorbs magnetic beads and nucleic acid molecules and then transfers the magnetic beads and the nucleic acid molecules to a storage dish is realized, the utility model has convenient transportation and convenient operation for workers, and the full-automatic operation of the nucleic acid extractor is realized through the matching use of clicking and a speed reducing motor;
the existing stirring sleeve is arranged at the bottom of the mounting plate, and the mounting plate and the bracket are locked and fixed through a driving structure, although the mounting plate and the stirring sleeve can be fixedly arranged, generally, the driving structure has higher cost and more invested funds, if the driving structure is damaged in the using process, so that the locking structure in the stirring device can not lock the mounting plate, the locking structure can not lock and fix the stirring sleeve, so that the mounting plate and the stirring sleeve connected with the mounting plate are separated from the bracket, the stirring sleeve falls down, cause certain influence to the quality of stirring cover, shortened the life of stirring cover, increased the input of cost to a certain extent, reduced the stability of stirring cover installation, reduced the availability factor of stirring cover, for this reason, we propose full-automatic nucleic acid extraction purification device for the appearance.
Disclosure of Invention
According to the technical problems, the utility model provides a purification device for a full-automatic nucleic acid extraction instrument, which is characterized by comprising a stirring sleeve structure, wherein the stirring sleeve structure comprises a frame, sliding plates, sliding blocks, mounting plates, a stirring sleeve, a mounting cylinder, a locking rod, a connecting block, a spring, a limiting rod, a through plate and a limiting component, the frame is of a structure shaped like a Chinese character 'hui', the sliding plates are respectively arranged at the left end and the right end of the bottom of the frame, the sliding plates are of an L-shaped structure, the sliding blocks are respectively arranged on the inner side walls of the two sliding plates, the mounting plate is arranged between the two sliding blocks, a positioning hole is arranged in the mounting plate, the stirring sleeve is arranged in the positioning hole, the buffer components are respectively arranged at the front end and the rear end of the bottom of the frame, the buffer components are in contact with the mounting plate, the mounting cylinder is arranged on the outer side wall of the sliding plate at the left side, the left side and the right side of the mounting cylinder are respectively provided with a through hole, the insides of the sliding plate, the sliding block and the mounting plate are respectively provided with a locking hole, the locking hole and the through hole are arranged on the same straight line, the inside of the mounting cylinder is provided with a locking rod, one end of the locking rod passes through the through hole of the left side wall of the mounting cylinder and extends to the outside to be provided with a handle, the other end of the locking rod passes through the locking hole and extends to the outside of the sliding plate, the outer side wall of the locking rod is sleeved with a connecting block, the connecting block is arranged inside the mounting cylinder, the outer side wall of the locking rod is wound with a spring, one end of the spring is connected with the outer side wall of the connecting block, the other end of the spring is connected with the left sliding plate, the right end of the locking rod is provided with a limiting hole, the outer side wall of the right sliding plate is provided with a through plate, the inside of the through plate is provided with a mounting hole, and the mounting hole and the limiting hole are arranged on the same straight line, the bottom of the through plate is provided with an installation cylinder, a limiting rod is arranged in the installation cylinder, one end of the limiting rod extends to the outer side of the installation cylinder and is provided with a handle, the other end of the limiting rod penetrates through the installation hole and the limiting hole and extends to the upper side of the locking rod, and the upper end of the inner part of the limiting rod is provided with a limiting assembly;
the limiting component consists of a limiting block, a pulling block, a compressing block, a compression spring, a limiting plate, a clamping ring and a clamping groove, a through groove is arranged inside the limiting rod, adjusting holes are symmetrically arranged inside the limiting rod, the adjusting holes are arranged at two sides of the through groove and communicated with the through groove, a compression spring is arranged in the adjusting holes, one end of the compression spring is provided with a compression block, the other end of the compression spring is provided with a limit plate, the limiting plate is contacted with the outer side wall of the locking rod and extends to the outer side of the limiting rod, the side wall of the compression block is provided with a clamping groove, the inside of the through groove is provided with a limiting block, the outer side wall of the limiting block is provided with a snap ring, the clamping ring is made of elastic materials, the clamping ring is matched with the clamping groove for use, the top of the limiting block is provided with a pulling block, and the pulling block is arranged on the outer side of the limiting rod;
the buffer assembly comprises a buffer spring, a transition plate, press blocks and a protective layer, wherein the buffer spring is respectively arranged at the front end and the rear end of the bottom of the frame, the transition plate is arranged at the bottom of the buffer spring, the press blocks are arranged at the bottom of the transition plate, the number of the press blocks is N, N is more than or equal to 2, the press blocks are in a trapezoidal structure, the protective layer is arranged at the bottom of the press blocks, and the protective layer is made of rubber;
the inner side wall of the sliding block is provided with a fixing groove, balls are mounted in the fixing groove, the number of the balls is M, M is larger than or equal to 2, and the balls are in contact with the side wall of the sliding plate.
The utility model has the advantages that: the utility model realizes the sliding installation of the mounting plate and the frame by arranging the mounting cylinder, the locking rod, the connecting block, the spring, the limiting rod and the limiting component, and realizes the fixed installation of the sliding plate, the slider and the mounting plate by the matching use of the sliding block and the sliding plate in the application, the locking rod in the locking hole realizes the effective limiting of the position of the locking rod under the action of the spring force, the limiting plate is in direct contact with the locking rod, so that the limiting plate plays a certain limiting locking role on the limiting rod, the separation of the locking rod and the limiting rod in the mounting process of the mounting plate can be effectively prevented, the phenomenon that the stirring sleeve falls down can be caused, the quality of the stirring sleeve can not be influenced to a certain extent, the service life is prolonged, and the stability of the stirring sleeve and the mounting plate can be improved to a certain extent, the operation of replacing and disassembling the stirring sleeve and the mounting plate is realized, compared with the existing mode of replacing by adopting a driving device, a large amount of economic cost is saved, the cost input is reduced, and the use efficiency of the stirring sleeve is improved;
the utility model has the advantages that the buffering assembly consisting of the buffering spring, the transition plate, the pressing block and the protective layer is arranged, so that the mounting plate and the stirring sleeve in the working process can be effectively buffered, and the protective layer made of rubber can protect the surface of the mounting plate;
the utility model discloses lateral wall at the slider sets up the ball for ball and sliding plate direct contact have improved slider slip operation's in the sliding plate stability to a certain extent, make its slip in-process the condition that the card went up can not appear, make the installation more smoothly unimpededly, have further improved the stability of installation.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the structure of the stirring sleeve of the present invention;
fig. 3 is a schematic view of the limiting assembly of the present invention;
FIG. 4 is an enlarged view of the position A of the present invention;
FIG. 5 is an enlarged view of the position B of the present invention;
fig. 6 is an enlarged view of the position C of the present invention.
As shown in the figure, the device comprises a body 1, a magnetic rod mechanism 2, a stirring sleeve structure 3, a frame 30, a buffer spring 300, a transition plate 301, a pressing block 302, a protective layer 303, a sliding plate 31, a sliding block 32, a fixed groove 320, a ball 321, a mounting plate 33, a stirring sleeve 34, a mounting barrel 35, a locking rod 36, a connecting block 360, a spring 361, a limiting rod 37, a penetrating plate 38, a limiting component 39, a limiting block 390, a pulling block 391, a compression block 392, a compression spring 393, a limiting plate 395, a clamping ring 396 and a purification structure 4.
Detailed Description
Example 1
The utility model provides a purification device for a full-automatic nucleic acid extraction instrument, which is characterized by comprising a stirring sleeve structure 3, wherein the stirring sleeve structure 3 comprises a frame 30, a sliding plate 31, a sliding block 32, a mounting plate 33, a stirring sleeve 34, a mounting cylinder 35, a locking rod 36, a connecting block 360, a spring 361, a limiting rod 37, a through plate 38 and a limiting component 39, the frame 30 is of a structure shaped like a Chinese character 'hui', the sliding plates 31 are respectively arranged at the left end and the right end of the bottom of the frame 30, the sliding plates 31 are of an 'L' structure, the sliding blocks 32 are respectively arranged on the inner side walls of the two sliding plates 31, the mounting plate 33 is arranged between the two sliding blocks 32, a positioning hole is arranged inside the mounting plate 33, the stirring sleeve 34 is arranged in the positioning hole, buffer components are respectively arranged at the front end and the rear end of the bottom of the frame 30, the buffer components are in contact with the mounting plate 33, the mounting cylinder 35 is arranged on the outer side wall of the sliding plate 31 at the left side, perforations are respectively arranged at the left side edge of the mounting cylinder 35, the sliding plate 31, the sliding block 32 and the mounting plate 33 are respectively provided with a locking hole inside, the locking hole and the through hole are arranged on the same straight line, the mounting cylinder 35 is internally provided with a locking rod 36, one end of the locking rod 36 passes through the through hole on the left side wall of the mounting cylinder 35 and extends to the outside to be provided with a handle, the other end of the locking rod 36 passes through the locking hole and extends to the outside of the sliding plate 31, the outer side wall of the locking rod 36 is sleeved with a connecting block 360, the connecting block 360 is arranged inside the mounting cylinder 35, the outer side wall of the locking rod 36 is wound with a spring 361, one end of the spring 361 is connected with the outer side wall of the connecting block 360, the other end of the spring 361 is connected with the left sliding plate 31, the right end of the locking rod 36 is provided with a limiting hole, the outer side wall of the right sliding plate 31 is provided with a penetrating plate 38, the inside of, a limiting rod 37 is arranged in the mounting cylinder 35, one end of the limiting rod 37 extends to the outer side of the mounting cylinder 35 and is provided with a handle, the other end of the limiting rod 37 penetrates through the mounting hole and the limiting hole and extends to the upper side of the locking rod 36, and a limiting component 39 is arranged at the upper end of the inner part of the limiting rod 37;
the limiting component 39 consists of a limiting block 390, a pulling block 391, a compressing block 392, a compressing spring 393361, a limiting plate 394, a snap ring 395 and a clamping groove 396, a through groove is arranged inside the limiting rod 37, adjusting holes are symmetrically arranged inside the limiting rod 37, the adjusting holes are formed in two sides of the through groove and communicated with the through groove, a compression spring 393361 is installed inside the adjusting holes, a compression block 392 is installed at one end of the compression spring 393361, a limiting plate 394 is installed at the other end of the compression spring 393361, the limiting plate 394 is in contact with the outer side wall of the locking rod 36, the limiting plate 394 extends to the outer side of the limiting rod 37, a clamping groove 396 is formed in the side wall of the compression block 392, a limiting block 390 is installed inside the through groove, a clamping ring 395 is arranged on the outer side wall of the limiting block 390, the clamping ring 395 is made of elastic materials, the clamping ring 395 is matched with the clamping groove 396 for use, a pulling block 391 is installed at the top of the limiting block 390, and the pulling block 391 is installed on the outer side of the limiting rod 37;
the buffer assembly consists of a buffer spring 300, a transition plate 301, pressing blocks 302 and a protective layer 303, the buffer spring 300 is respectively arranged at the front end and the rear end of the bottom of the frame, the transition plate 301 is arranged at the bottom of the buffer spring 300, the pressing blocks 302 are arranged at the bottom of the transition plate 301, the number of the pressing blocks 302 is N, N is more than or equal to 2, the pressing blocks 302 are in a trapezoid structure, the protective layer 303 is arranged at the bottom of the pressing blocks 302, and the protective layer 303 is made of rubber;
the inner side wall of the sliding block 32 is provided with a fixing groove 320, the fixing groove 320 is provided with balls 321, the number of the balls 321 is M, M is more than or equal to 2, and the balls 321 are in contact with the side wall of the sliding plate 31.
Example 2
The stirring sleeve structure 3 in the utility model is arranged between the magnetic bar mechanism 2 and the purification structure 4; when the mounting plate 33 and the stirring sleeve 34 need to be mounted, firstly, the locking rod 36 is pulled to the outer side of the mounting cylinder 35 under the action of the elastic force of the spring 361, so that the locking rod is pulled to the outer side of the sliding plate 31, the sliding blocks 32 on both sides of the mounting plate 33 are inserted into the sliding plate 31 and slide towards the inner side of the sliding plate, the balls 321 are in direct contact with the sliding plate 31, the stability of the sliding operation of the sliding blocks 32 in the sliding plate 31 is improved to a certain extent, the clamping situation cannot occur in the sliding process, so that the mounting process is smoother and smoother, in the sliding process, the front end and the rear end of the mounting plate 33 are in contact with the pressing block 302, and the pressing block 302 is pressed upwards, so that the pressing block 302 and the transition plate 301 compress the buffer spring 300, the mounting plate 33 and the stirring sleeve 34 perform the sliding or working process, the buffer spring 300 can effectively buffer the mounting plate, and the protective layer 303 made of rubber can protect the surface of the mounting plate 33, the locking rod 36 is loosened, the locking rod 36 is inserted into the locking hole under the action of the elastic force of the spring 361 to realize locking, the limiting rod 37 is inserted into the limiting hole to limit the position of the locking rod 36, the pulling block 391 is pressed downwards to enable the limiting block 390 to move, the pressing block 302 moves into the adjusting hole, the mounting of the limiting block 390 and the pressing block 302 is realized through the matching use of the clamping ring 395 and the clamping groove 396, the limiting plate 394 is further driven to move towards the outer side of the limiting rod 37, the limiting rod 37 is in contact with the locking rod 36 to realize effective limiting of the position of the limiting rod 37, the locking and fixing of the mounting plate 33 and the stirring sleeve 34 are realized through the matching use of the locking rod 36, the spring 361, the limiting component 39 and the limiting rod 37, the operation of replacing and disassembling the stirring sleeve 34 and the mounting plate 33 is realized, compared with the existing replacement mode adopting a driving device, saving a large amount of economic cost, reducing the investment of cost and improving the use efficiency of the stirring sleeve 34.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. The utility model discloses each part that mentions is the common technique in prior art, and the technical personnel of this trade should understand, the utility model discloses do not receive the restriction of above-mentioned embodiment, the description only is the explanation in above-mentioned embodiment and the description the principle of the utility model, under the prerequisite that does not deviate from the spirit and the scope of the utility model, the utility model discloses still can have various changes and improvement, these changes and improvement all fall into the protection of claim the utility model is within the scope. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (7)

1. The purification device for the full-automatic nucleic acid extractor is characterized by comprising a stirring sleeve structure, wherein the stirring sleeve structure consists of a frame, sliding plates, sliding blocks, a mounting plate, a stirring sleeve, a mounting cylinder, a locking rod, a connecting block, a spring, a limiting rod, a through plate and a limiting component, the sliding plates are respectively mounted at the left end and the right end of the bottom of the frame, the sliding blocks are respectively mounted on the inner side walls of the two sliding plates, the mounting plate is mounted between the two sliding blocks, a positioning hole is formed in the mounting plate, the stirring sleeve is arranged in the positioning hole, the front end and the rear end of the bottom of the frame are respectively mounted with a buffering component, the buffering component is contacted with the mounting plate, the mounting cylinder is mounted on the outer side wall of the sliding plate at the left side, the left side edge and the right side edge of the mounting cylinder are respectively provided with a through hole, the locking holes are respectively formed in the sliding plates, the sliding blocks and the mounting plate, the locking hole and the through hole are arranged on the same straight line, a locking rod is arranged in the mounting cylinder, one end of the locking rod passes through the through hole on the left side wall of the mounting cylinder and extends to the outside to be provided with a handle, the other end of the locking rod passes through the locking hole and extends to the outside of the sliding plate, a connecting block is sleeved on the outer side wall of the locking rod, the connecting block is arranged in the mounting cylinder, a spring is wound on the outer side wall of the locking rod, one end of the spring is connected with the outer side wall of the connecting block, the other end of the spring is connected with the left side sliding plate, a limiting hole is arranged at the right end of the locking rod, a penetrating plate is arranged on the outer side wall of the right side sliding plate, a mounting hole is arranged in the penetrating plate, the mounting hole and the limiting hole are arranged on the same straight line, the mounting cylinder is arranged at the bottom of the penetrating plate, and the limiting rod is arranged in the mounting cylinder, one end of the limiting rod extends to the outer side of the mounting cylinder and is provided with a handle, the other end of the limiting rod penetrates through the mounting hole and the limiting hole and extends to the upper side of the locking rod, and a limiting assembly is arranged at the upper end of the inner part of the limiting rod;
the limiting assembly comprises a limiting block, a pulling block, a compression spring, a limiting plate, a clamping ring and a clamping groove, wherein a through groove is formed in the limiting rod, adjusting holes are symmetrically formed in the limiting rod and are formed in two sides of the through groove, the adjusting holes are communicated with the through groove, the compression spring is installed in the adjusting holes, the compression block is installed at one end of the compression spring, the limiting plate is installed at the other end of the compression spring, the limiting plate is in contact with the outer side wall of the locking rod, the limiting plate extends to the outer side of the limiting rod, the clamping groove is formed in the side wall of the compression block, the limiting block is installed in the through groove, the clamping ring is arranged on the outer side wall of the limiting block, the clamping ring is matched with the clamping groove for use, the pulling block is installed at the top of the limiting block, and the pulling block is installed on the outer side of the limiting rod;
the buffer assembly consists of a buffer spring, a transition plate, a pressing block and a protective layer, wherein the buffer spring is respectively arranged at the front end and the rear end of the bottom of the frame, the transition plate is arranged at the bottom of the buffer spring, the pressing block is arranged at the bottom of the transition plate, and the protective layer is arranged at the bottom of the pressing block;
the inner side wall of the sliding block is provided with a fixing groove, a ball is mounted in the fixing groove, and the ball is in contact with the side wall of the sliding plate.
2. The purification apparatus for a full-automatic nucleic acid extractor according to claim 1, wherein the frame has a structure of a shape like a Chinese character 'hui'.
3. The purification apparatus for a fully automatic nucleic acid extractor according to claim 1, wherein the slide plate has an "L-shaped" structure.
4. The purification apparatus for a full-automatic nucleic acid extractor according to claim 1, wherein the retainer ring is made of an elastic material.
5. The purification device for the full-automatic nucleic acid extractor according to claim 1, wherein the number of the briquettes is N, N is not less than 2, and the briquettes have a trapezoidal structure.
6. The purification device for a full-automatic nucleic acid extractor according to claim 1, wherein the protective layer is made of rubber.
7. The purification apparatus for a full-automatic nucleic acid extractor according to claim 1, wherein the number of said balls is M, M.gtoreq.2.
CN202022198083.3U 2020-09-30 2020-09-30 Purification device for full-automatic nucleic acid extraction instrument Expired - Fee Related CN213951136U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022198083.3U CN213951136U (en) 2020-09-30 2020-09-30 Purification device for full-automatic nucleic acid extraction instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022198083.3U CN213951136U (en) 2020-09-30 2020-09-30 Purification device for full-automatic nucleic acid extraction instrument

Publications (1)

Publication Number Publication Date
CN213951136U true CN213951136U (en) 2021-08-13

Family

ID=77202600

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022198083.3U Expired - Fee Related CN213951136U (en) 2020-09-30 2020-09-30 Purification device for full-automatic nucleic acid extraction instrument

Country Status (1)

Country Link
CN (1) CN213951136U (en)

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Granted publication date: 20210813