CN213950557U - Cell suspension sterile liquid-separating and subpackaging device - Google Patents
Cell suspension sterile liquid-separating and subpackaging device Download PDFInfo
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- CN213950557U CN213950557U CN202022906909.7U CN202022906909U CN213950557U CN 213950557 U CN213950557 U CN 213950557U CN 202022906909 U CN202022906909 U CN 202022906909U CN 213950557 U CN213950557 U CN 213950557U
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Abstract
The utility model relates to the field of cell preparation, in particular to an aseptic liquid-separating and subpackaging device for cell suspension, which comprises a sealed cell suspension mother liquid storage bottle, wherein the mother liquid storage bottle is communicated with a gas pressurizing device used for pressurizing the interior of the mother liquid storage bottle through a first connecting pipe; the mother liquid storage bottle is communicated with the inlet end of the first three-way connecting pipe through a second connecting pipe; the end part of one end of the second connecting pipe, which extends into the mother liquid storage bottle, is arranged at the bottom of the mother liquid storage bottle; two outlet ends of the first three-way connecting pipe are respectively communicated with two sealed cell suspension liquid-separating storage bottles of a third connecting pipe; the cell suspension liquid separation storage bottle further comprises a connecting pipe blocking device, and when the cell suspension liquid separation storage bottle reaches the liquid separation liquid amount, the connecting pipe blocking device blocks any one or more connecting pipes. The utility model discloses a to the convenient quick aseptic, the high-efficient partial shipment that expands the volume of the cell suspension that has prepared, eliminated drawbacks such as artificial pollution.
Description
Technical Field
The utility model relates to a cell preparation field especially relates to an aseptic transfer device of cell culture liquid.
Background
When the prepared cell suspension mother liquor (total liquor) is subpackaged into a sterile culture bottle, the traditional mode is to use a pipette to operate through a bottle opening or directly pour, but the pipette is single-channel subpackaging equipment, has low subpackaging efficiency and long time and is not suitable for large-scale production; the direct fungus contamination probability of empting is big, and still can lead to the waste of liquid medium, increases manufacturing cost, and can not guarantee to divide the liquid volume balanced accuracy, easily causes cell culture bottle unit effect difference great, appears the phenomenon of not reaching standard even, causes bigger waste. With the development of biopharmaceutical technology, the wide application and the continuous scale-up of cell culture technology, and in contrast, the volume of a bioreactor or a culture bottle is also larger and larger, and the traditional method cannot meet the expectations of producers and is not suitable for practical requirements, and the scheme is a device for solving the practical disadvantages and problems.
Disclosure of Invention
An object of the utility model is to avoid prior art's not enough to provide a sealed aseptic, high-efficient expansion, and simple structure, convenient to use, aseptic minute liquid partial shipment device of cell suspension with low costs.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a cell suspension sterile liquid-separating and subpackaging device, which comprises a sealed cell suspension mother liquid storage bottle,
the mother liquor storage bottle is communicated with a gas pressurizing device used for pressurizing the interior of the mother liquor storage bottle through a first connecting pipe;
the mother liquid storage bottle is communicated with the inlet end of the first three-way connecting pipe through a second connecting pipe; the end part of one end of the second connecting pipe, which extends into the mother liquid storage bottle, is arranged at the bottom of the mother liquid storage bottle;
two outlet ends of the first three-way connecting pipe are respectively communicated with two sealed cell suspension liquid-separating storage bottles of a third connecting pipe;
the cell suspension liquid separation storage bottle further comprises a connecting pipe blocking device, and when the cell suspension liquid separation storage bottle reaches the liquid separation liquid amount, the connecting pipe blocking device blocks any one or more connecting pipes.
Furthermore, two outlet ends of the three-way connecting pipe are respectively communicated with inlet ends of a second three-way connecting pipe and a third three-way connecting pipe through a third connecting pipe, and four outlet ends of the second three-way connecting pipe and the third three-way connecting pipe are respectively communicated with four sealed cell suspension liquid separation storage bottles through fourth connecting pipes.
Furthermore, the included angle between the inlet end pipeline of the three-way connecting pipe and the two outlet end pipelines is 90-120 degrees, and the included angle between the two outlet end pipelines is 120-180 degrees.
Furthermore, the gas pressurizing device is a manual pressurizing latex ball or an electric air pump.
Furthermore, the connecting pipe blocking device is a pipeline clamp or a hose flow rate regulator.
Furthermore, the first three-way connecting pipe, the third connecting pipe and two sealed cell suspension liquid-separating storage bottles communicated with the third connecting pipe are used as a liquid-separating unit, and the first connecting pipe is continuously communicated with a plurality of liquid-separating units.
Furthermore, the first three-way connecting pipe, the third connecting pipe and two sealed cell suspension liquid-separating storage bottles communicated with the third connecting pipe are used as one liquid-separating unit, and a plurality of liquid-separating units are continuously arranged in the third connecting pipe or the fourth connecting pipe in a communicated manner, so that a multi-stage liquid-separating unit with the first connecting pipe as a first-stage connecting pipe and the second connecting pipe and the fourth connecting pipe as second-stage and third-stage connecting pipes is formed.
Furthermore, an exhaust pipe is arranged on the cell suspension split charging bottle.
Furthermore, the three-way connecting pipe is a stainless steel pipe, the connecting pipe is a sanitary silicone tube, and the connecting pipe and the three-way connecting pipe are arranged in a split manner; the three-way connecting pipe and the connecting pipe are sanitary silicone tubes and are integrally arranged.
Furthermore, an air filtering and sterilizing device is arranged on a first connecting pipe between the mother liquid storage bottle and the gas pressurizing device. The air filtering and sterilizing device can adopt a plurality of air filtering and sterilizing devices such as an air filtering rod or a filter plate.
The utility model has the advantages that: the utility model discloses a to the convenient quick aseptic, the high-efficient partial shipment that expands the volume of the cell suspension that has prepared, eliminated drawbacks such as artificial pollution.
Drawings
Fig. 1 is a schematic structural view of embodiment 1 of the present invention;
fig. 2 is a schematic structural view of embodiment 2 of the present invention;
fig. 3 is a schematic structural view of a three-way connection pipe of the present invention;
fig. 4 is a schematic structural diagram of embodiment 5 of the present invention;
fig. 5 is a schematic structural view of embodiment 6 of the present invention.
Detailed Description
The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.
Example 1: as shown in fig. 1, the cell suspension sterile liquid separating and subpackaging device comprises a sealed cell suspension mother liquid storage bottle 1, wherein the mother liquid storage bottle 1 is communicated with a gas pressurizing device 3 for pressurizing the interior of the mother liquid storage bottle 1 through a first connecting pipe 21; the mother liquid storage bottle 1 is communicated with the inlet end of a first three-way connecting pipe 41 through a second connecting pipe 22; the end part of one end of the second connecting pipe 22 extending into the mother liquor storage bottle 1 is arranged at the bottom of the mother liquor storage bottle 1; two outlet ends of the first three-way connecting pipe 41 are respectively communicated with the two sealed cell suspension liquid-separating storage bottles 5 through a third connecting pipe 23; the cell suspension liquid separation storage bottle is characterized by further comprising a connecting pipe blocking device 6, and when the cell suspension liquid separation storage bottle 5 reaches the liquid separation liquid amount, the connecting pipe blocking device 6 blocks any one or more connecting pipes. An exhaust pipe 26 is arranged on the cell suspension split bottle 5, so that the split is smoother. An air filtering and sterilizing device 7 is also arranged on the first connecting pipe 21 between the mother liquor storage bottle 1 and the gas pressurizing device 3.
The pipeline included angle between the inlet end pipeline of the first three-way connecting pipe 41 and the two outlet end pipelines is 90 degrees, and the pipeline included angle between the two outlet end pipelines is 180 degrees. The gas pressurizing device 3 is a manual pressurizing latex ball. The connecting pipe blocking device 6 is a pipeline clamp. The first three-way connecting pipe 41 is a stainless steel pipe, and the three connecting pipes are sanitary silicone tubes. The second connecting pipe and the third connecting pipe are arranged separately from the first three-way connecting pipe. The rubber connecting pipe and the stainless steel three-way connecting pipe can be communicated in various forms, such as bonding or iron wires.
In use, every bottle of cell suspension mother liquor storage bottle 1 of general prophase preparation is in 15000-.
When the cell suspension mother liquor needs to be separated into bottles, the gas pressurizing device 3 is used for inflating and pressurizing the cell suspension mother liquor storage bottle 1, at the moment, the suspension mother liquor in the cell suspension mother liquor storage bottle 1 enters the inlet of the first three-way connecting pipe 41 through the second connecting pipe 22, meanwhile, the suspension mother liquor continues to enter the two cell suspension liquid separation storage bottles 5 from the two outlets of the first three-way connecting pipe 41 and through the third connecting pipe 23, when one cell suspension liquid separation storage bottle 5 reaches 1000-1500 ml, the connecting pipe blocking device 6 is used for blocking either side of the third connecting pipe 23, when the two cell suspension liquid separation storage bottles 5 reach 1000-1500 ml, the connecting pipe blocking device 6 can be used for blocking the third connecting pipe 23 or the second connecting pipe 22, and the liquid separation of the cell suspension mother liquor is completed.
Example 2: as shown in fig. 2, the same as in example 1, except that two outlet ends of the first three-way connecting tube 41 are respectively communicated with inlet ends of a second three-way connecting tube 42 and a third three-way connecting tube 43 through a third connecting tube 23, and four outlet ends of the second three-way connecting tube 42 and the third three-way connecting tube 43 are respectively communicated with four sealed cell suspension liquid-separating storage bottles 5 through fourth connecting tubes 24. The cell suspension split charging bottle 5 is also provided with an exhaust pipe 26. An air filtering and sterilizing device 7 is also arranged between the mother liquor storage bottle 1 and the gas pressurizing device 3 through a first connecting pipe 21.
The pipeline included angle between the inlet end pipeline of the first three-way connecting pipe 41 and the two outlet end pipelines is 90 degrees, and the pipeline included angle between the two outlet end pipelines is 180 degrees. The gas pressurizing device 3 is an electric air pump. The connecting pipe blocking device 6 is a pipe clamp and a hose flow rate regulator, and the hose flow rate regulator is an existing product used in the infusion device. The three-way connecting pipes in the embodiment are all sanitary silicone tubes. The connecting pipe is a sanitary silicone tube. The connecting pipe and the three-way connecting pipe are arranged integrally.
When the cell suspension mother liquor needs to be separated into bottles, the gas pressurizing device 3 is used for inflating and pressurizing the cell suspension mother liquor storage bottle 1, at the moment, the suspension mother liquor in the cell suspension mother liquor storage bottle 1 enters the inlet of the first three-way connecting pipe 41 through the second connecting pipe 22, meanwhile, the suspension mother liquor continues to enter the inlets of the second three-way connecting pipe 42 and the third three-way connecting pipe 43 through the two outlets of the first three-way connecting pipe 41 and the third connecting pipe 23, the four outlet ends of the second three-way connecting pipe 42 and the third three-way connecting pipe 43 are respectively communicated with the four sealed cell suspension liquid separating storage bottles 5, when one of the cell suspension liquid separating storage bottles 5 reaches 1000-1500 ml, the connecting pipe blocking device 6 is used for blocking the third connecting pipe 23 or the fourth connecting pipe 24, when the two cell suspension liquid separating storage bottles 5 both reach 1000-1500 ml, the connecting pipe blocking device 6 can be used for blocking the third connecting pipe 23, the fourth connecting pipe 24 or the second connecting pipe 22, so that the liquid separation and the subpackage of the cell suspension mother liquor can be completed.
Example 3: as shown in fig. 3, the same as in example 1, except that: the pipeline included angle alpha between the inlet end pipeline of the three-way connecting pipe 4 and the two outlet end pipelines is 120 degrees, and the pipeline included angle between the two outlet end pipelines is 120 degrees.
Example 4: as shown in fig. 3, the same as in example 2, except that: the pipeline included angle alpha between the inlet end pipeline of the three-way connecting pipe 4 and the two outlet end pipelines is 120 degrees, and the pipeline included angle between the two outlet end pipelines is 120 degrees.
Example 5: as shown in fig. 4, the same as in example 1, except that: the first three-way connecting pipe 41, the third connecting pipe 23 and the two sealed cell suspension liquid-separating storage bottles 5 communicated with the third connecting pipe 23 in the embodiment 1 are used as a liquid-separating unit, and a plurality of liquid-separating units are continuously communicated and arranged on the first connecting pipe 22.
Example 6: as shown in fig. 5, the same as in example 2, except that: the first three-way connecting pipe 41, the third connecting pipe 23 and the two sealed cell suspension liquid-separating storage bottles 5 communicated with the third connecting pipe 23 in the embodiment 2 are used as a liquid-separating unit, the liquid-separating unit is continuously extended step by step in the third connecting pipe 23 or the fourth connecting pipe 24, that is, the first connecting pipe 22 is a first-stage connecting pipe, the second connecting pipe 23 and the fourth connecting pipe 24 are respectively a second-stage connecting pipe and a third-stage connecting pipe, one of the fourth connecting pipes 24 is communicated with the inlet of the fourth three-way connecting pipe 44, the outlet of the fourth three-way connecting pipe 44 is communicated with the two sealed cell suspension liquid-separating storage bottles 5 through the fifth connecting pipe 25, at this time, the fifth connecting pipe 25 is a fourth-stage connecting pipe, and a plurality of separated bottles of suspension mother liquor are sequentially extended according to the above.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.
Claims (10)
1. An aseptic separating and subpackaging device for cell suspension is characterized by comprising a sealed cell suspension mother liquor storage bottle (1),
the mother liquor storage bottle (1) is communicated with a gas pressurizing device (3) used for pressurizing the interior of the mother liquor storage bottle (1) through a first connecting pipe (21);
the mother liquor storage bottle (1) is communicated with the inlet end of a first three-way connecting pipe (41) through a second connecting pipe (22); one end part of the second connecting pipe (22) extending into the mother liquor storage bottle (1) is arranged at the bottom of the mother liquor storage bottle (1);
two outlet ends of the first three-way connecting pipe (41) are respectively communicated with the two sealed sterile cell suspension split bottles (5) through a third connecting pipe (23);
the cell suspension split charging device also comprises a connecting pipe blocking device (6), and when the cell suspension split charging bottle (5) reaches the split charging liquid amount, the connecting pipe blocking device (6) blocks any one or more connecting pipes.
2. The sterile liquid-separating and dispensing device for cell suspension liquid according to claim 1, wherein two outlet ends of the first three-way connecting pipe (41) are respectively communicated with the inlet ends of the second three-way connecting pipe (42) and the third three-way connecting pipe (43) through a third connecting pipe (23), and four outlet ends of the second three-way connecting pipe (42) and the third three-way connecting pipe (43) are respectively communicated with four sealed cell suspension liquid-dispensing bottles (5) through a fourth connecting pipe (24).
3. The sterile liquid-separating and dispensing device for cell suspension liquid as claimed in claim 1, wherein the included angle between the inlet end tube and the two outlet end tubes of the three-way connecting tube is 90-120 degrees, and the included angle between the two outlet end tubes is 120-180 degrees.
4. The cell suspension sterile liquid-separating and split charging device according to claim 1, wherein the gas pressurizing device (3) is a manually pressurized latex ball or an electric air pump.
5. The sterile liquid-separating and dispensing device for cell suspension liquid according to claim 1, wherein the connecting pipe blocking device (6) is a pipe clamp or a hose flow rate regulator.
6. The sterile device for separating and dispensing cell suspension according to claim 1, wherein the first three-way connecting tube (41), the third connecting tube (23) and the two sealed cell suspension separating bottles (5) communicated with the third connecting tube (23) are used as one separating unit, and a plurality of separating units are continuously communicated with the first connecting tube (21).
7. The cell suspension sterile liquid-separating and split-charging device according to claim 2, wherein the first three-way connecting pipe (41), the third connecting pipe (23) and the two sealed cell suspension liquid-separating bottles (5) communicated with the third connecting pipe (23) are used as one liquid-separating unit, a plurality of liquid-separating units are continuously communicated and arranged on the third connecting pipe (23) or the fourth connecting pipe (24), so that the first connecting pipe (21) is used as a first-stage connecting pipe, and the second connecting pipe (22) and the fourth connecting pipe (24) are respectively used as multi-stage liquid-separating units of second-stage and third-stage connecting pipes.
8. The cell suspension sterile liquid-separating and dispensing device according to any one of claims 1 to 7, wherein an exhaust pipe (26) is further provided on the cell suspension dispensing bottle (5).
9. The sterile liquid-separating and subpackaging device for the cell suspension liquid according to any one of claims 1 to 7, wherein the three-way connecting pipe is a stainless steel pipe, the connecting pipe is a sanitary silicone tube, and the connecting pipe and the three-way connecting pipe are arranged in a split manner; the three-way connecting pipe and the connecting pipe are sanitary silicone tubes and are integrally arranged.
10. The sterile dispensing device for separating liquid of cell suspension according to any one of claims 1-7, characterized in that an air filtration and sterilization device (7) is further arranged on the first connecting pipe (21) between the mother liquid storage bottle (1) and the gas pressurizing device (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022906909.7U CN213950557U (en) | 2020-12-07 | 2020-12-07 | Cell suspension sterile liquid-separating and subpackaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022906909.7U CN213950557U (en) | 2020-12-07 | 2020-12-07 | Cell suspension sterile liquid-separating and subpackaging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213950557U true CN213950557U (en) | 2021-08-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022906909.7U Active CN213950557U (en) | 2020-12-07 | 2020-12-07 | Cell suspension sterile liquid-separating and subpackaging device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213950557U (en) |
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- 2020-12-07 CN CN202022906909.7U patent/CN213950557U/en active Active
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