CN213924873U

CN213924873U - Reactor for large-scale production of suspension cells

Info

Publication number: CN213924873U
Application number: CN202022993346.XU
Authority: CN
Inventors: 谭秀庆; 叶发才; 孙杨; 余斌
Original assignee: Chengdu Yingde Bio Medical Equipment Technology Co ltd
Current assignee: Chengdu Yingde Bio Medical Equipment Technology Co ltd
Priority date: 2020-12-14
Filing date: 2020-12-14
Publication date: 2021-08-10
Anticipated expiration: 2030-12-14

Abstract

The utility model discloses a reactor of scale production suspension cell, it is including jar body (1), culture medium pipeline (2), inoculation and PH adjusting line, exhaust pipe line (3), washing pipeline (4), pure compressed air pipeline (5), pure steam pipeline (6), carbon dioxide pipeline (7), oxygen pipeline (8), product outlet pipeline (9) and temperature control system, be connected with third valve (10) and tail gas filter (11) between exhaust pipe line (3) and the jar body (1) in order, the both ends of third valve (10) are parallelly connected have first valve (12) and second valve (13), and first valve (12) link to each other with second valve (13), wash pipeline (4) including set firmly in the thirty-seventh valve (14) of jar body (1) bottom. The utility model has the advantages that: compact structure, high production efficiency, small difference between product batches, capability of improving the suitable growth and metabolism environment for cells and capability of producing virus antigens in a large scale.

Description

Reactor for large-scale production of suspension cells

Technical Field

The utility model relates to the technical field of biological pharmacy equipment, especially a reactor of scale production suspension cell.

Background

The core link of animal vaccine production is the production and manufacture of virus antigens, the traditional bottle-rotating or chick embryo process has low production efficiency, high labor intensity and large batch difference, and the inherent defects cause poor product quality and high cost and can not meet the requirements of large-scale breeding customers. Foot-and-mouth disease is considered as the most contagious disease among the infectious diseases of livestock, and is classified as a type A infectious disease by the International veterinary agency (OIE). In 2010 and 2011, there were outbreaks of local foot and mouth disease in both asia and africa. Safe and efficient vaccine inoculation is one of the most effective strategies for preventing foot and mouth disease epidemic. The high efficiency of the foot-and-mouth disease vaccine is generally realized by mainly improving the effective antigen amount of the foot-and-mouth disease vaccine through a concentration technology; the safety of the vaccine is ensured by an effective virus inactivation process, but the preparation of the virus antigen by a concentration technology has the defects of small scale, low production efficiency and large difference between product batches. Therefore, a reactor for producing suspension cells in a large scale, which has high production efficiency and small difference between product batches and can improve the suitable growth and metabolism environment for the cells, is urgently needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects of the prior art and provide a reactor for mass production of suspended cells, which has compact structure, high production efficiency, small difference between batches of products and can improve the suitable growth and metabolism environment for the cells.

The purpose of the utility model is realized through the following technical scheme: a reactor for large-scale production of suspended cells comprises a tank body, a culture medium pipeline, an inoculation and PH regulation pipeline, an exhaust pipeline, a cleaning pipeline, a pure compressed air pipeline, a pure steam pipeline, a carbon dioxide pipeline, an oxygen pipeline, a product outlet pipeline and a temperature control system, wherein a third valve and a tail gas filter are sequentially connected between the exhaust pipeline and the tank body, a first valve and a second valve are connected at two ends of the third valve in parallel, the first valve is connected with the second valve, the cleaning pipeline comprises a thirty-seventh valve fixedly arranged at the bottom of the tank body, a seventh valve, a twenty-first valve, a twenty-thirteen valve, a twenty-fourth valve, a twenty-fifth valve, a twenty-sixth valve and a thirty-eighth valve are sequentially connected between the cleaning pipeline and the thirty-seventh valve, the twenty-third valve, the twenty-fourth valve and the twenty-fifth valve are all communicated with the top of the tank body, the bottoms of the twenty-fourth valve and the twenty-fifth valve are both connected with a spray ball extending into the tank body, the culture medium pipeline is connected with a sixth valve, the sixth valve is connected with a node between the seventh valve and the twenty-first valve, the pure compressed air pipeline is connected with an eleventh valve, a thirteenth valve and a seventeenth valve in parallel, the other end of the eleventh valve is sequentially connected with a first filter and an eighth valve, the eighth valve is connected with a node between the seventh valve and the twenty-first valve, the other end of the thirteenth valve is connected with a second filter between the tank body, the other end of the seventeenth valve is sequentially connected with a third filter, a twelfth valve and a forty-fourth valve, the other end of the forty-fourth valve is connected with a deep ventilation ring, the deep ventilation ring extends into the tank body and is arranged at the lower end part of the tank body, and the twelfth valve is connected with the twenty-first valve, the pure steam pipeline is connected with a twelfth valve, the other end of the twelfth valve is connected with a node between the first filter and the eleventh valve, the carbon dioxide pipeline is connected with a sixteenth valve, the sixteenth valve is connected with a node between the seventeenth valve and the third filter, the oxygen pipeline is connected with an eighteenth valve, the eighteenth valve is connected with a node between the seventeenth valve and the third filter, the product outlet pipeline is connected with a thirty-ninth valve, the other end of the thirty-ninth valve is connected with a thirty-seventh valve, the temperature control system comprises a first plate heat exchanger, a second plate heat exchanger and a jacket fixedly arranged outside the tank body, the outlet end of the first plate heat exchanger is connected with the inlet end of the second plate heat exchanger, a circulating pump is connected between the outlet end of the second plate heat exchanger and the jacket, and the node between the circulating pump and the second plate heat exchanger is sequentially connected with a thirty-fifth valve and a softened water pipe A wire; the inoculation and PH regulation pipeline comprises a peristaltic pump, a seed receiving bag and an alkali liquor storage tank, two stop valves are connected in parallel at a liquid pumping port of the peristaltic pump, one stop valve is connected with the inoculation bag, the other stop valve is connected with the alkali liquor storage tank, a twenty-seventh valve is connected at a liquid discharging port of the peristaltic pump, and the other end of the twenty-seventh valve is fixedly arranged at the top of the tank body; the bottom of the tank body is provided with a stirrer, an output shaft of the stirrer is connected with a blade extending into the tank body, the bottom of the tank body is provided with a temperature sensor, and the top of the tank body is provided with a pressure sensor.

And the tank body is provided with a weighing sensor.

And a PH probe and an oxygen probe are arranged on the side wall of the tank body.

The tail gas filter, the first filter, the second filter and the third filter are connected with a sewage discharge pipeline.

And a fourteenth valve is connected in parallel to the thirteenth valve.

And a node between the twenty-fifth valve and the twenty-sixth valve is connected with an outlet of the twenty-seventh valve.

A node between the thirty-seventh valve and the thirty-ninth valve is connected with a third valve.

The volume of the tank body is 2000-10000L.

The utility model has the advantages of it is following: the utility model discloses compact structure, production efficiency are high, the difference is little between the product batch, can improve suitable growth metabolic environment, can the large-scale production virus antigen for the cell.

Drawings

Fig. 1 is a schematic structural view of the present invention;

in the figure, 1-tank, 2-culture medium line, 3-exhaust gas line, 4-purge line, 5-pure compressed air line, 6-pure steam line, 7-carbon dioxide line, 8-oxygen line, 9-product outlet line, 10-third valve, 11-exhaust gas filter, 12-first valve, 13-second valve, 14-thirty-seventh valve, 15-seventh valve, 16-twenty-first valve, 17-twenty-third valve, 18-twenty-fourth valve, 19-twenty-fifth valve, 20-twenty-sixth valve, 21-thirty-eighth valve, 22-spray ball, 23-sixth valve, 24-eleventh valve, 25-thirteenth valve, 26-seventeenth valve, 27-a first filter, 28-an eighth valve, 29-a second filter, 30-a third filter, 31-a twelfth valve, 32-a forty-fourth valve, 33-a deep layer ventilation ring, 34-a twelfth valve, 35-a sixteenth valve, 36-an eighteenth valve, 37-a thirty-ninth valve, 38-a first plate heat exchanger, 39-a second plate heat exchanger, 40-a jacket, 41-a circulating pump, 42-a thirty-fifth valve, 43-a softened water pipeline, 44-a peristaltic pump, 45-a bag receiving, 46-an alkali liquor storage tank, 47-a twenty-seventh valve, 48-a stirrer, 49-a blade, 50-a temperature sensor, 51-a pressure sensor, 52-a weighing sensor and 53-a PH probe, 54-oxygen probe, 55-blowdown line, 56-fourteenth valve, 57-40 th valve.

Detailed Description

The invention will be further described with reference to the accompanying drawings, without limiting the scope of the invention to the following:

as shown in figure 1, the reactor for large-scale production of suspended cells comprises a tank body 1, a culture medium pipeline 2, an inoculation and PH regulation pipeline, an exhaust pipeline 3, a cleaning pipeline 4, a pure compressed air pipeline 5, a pure steam pipeline 6, a carbon dioxide pipeline 7, an oxygen pipeline 8, a product outlet pipeline 9 and a temperature control system, wherein the volume of the tank body 1 is 2000-10000L, a weighing sensor 52 is arranged on the tank body 1, a third valve 10 and a tail gas filter 11 are sequentially connected between the exhaust pipeline 3 and the tank body 1, a first valve 12 and a second valve 13 are connected in parallel at two ends of the third valve 10, the first valve 12 is connected with the second valve 13, the cleaning pipeline 4 comprises a thirty-seventh valve 14 fixedly arranged at the bottom of the tank body 1, and a seventh valve 15, a twenty-first valve 16 and a twenty-seventh valve 17 are sequentially connected between the cleaning pipeline 4 and the thirty-seventh valve 14, A twenty-fourth valve 18, a twenty-fifth valve 19, a twenty-sixth valve 20 and a thirty-eighth valve 21, wherein the twenty-fourth valve 17, the twenty-fourth valve 18 and the twenty-fifth valve 19 are all communicated with the top of the tank body 1, the bottoms of the twenty-fourth valve 18 and the twenty-fifth valve 19 are all connected with a spray ball 22 extending into the tank body 1, the culture medium pipeline 2 is connected with a sixth valve 23, the sixth valve 23 is connected with a node between the seventh valve 15 and the twenty-first valve 16, the pure compressed air pipeline 5 is connected in parallel with an eleventh valve 24, a thirteenth valve 25 and a seventeenth valve 26, the other end of the eleventh valve 24 is sequentially connected with a first filter 27 and an eighth valve 28, the eighth valve 28 is connected with a node between the seventh valve 15 and the twenty-first valve 16, the other end of the thirteenth valve 25 is connected with a second filter 29 between the tank body 1, the other end of the seventeenth valve 26 is connected with a third filter 30, a twentieth valve 31 and a forty-fourth valve 32 in sequence, the other end of the forty-fourth valve 32 is connected with a deep-layer ventilation ring 33, the deep-layer ventilation ring 33 extends into the tank 1 and is arranged at the lower end part of the tank 1, the twentieth valve 31 is connected with the twenty-first valve 16, the twelfth valve 34 is connected at the pure steam pipeline 6, the other end of the twelfth valve 34 is connected with a node between the first filter 27 and the eleventh valve 24, the sixteenth valve 35 is connected at the carbon dioxide pipeline 7, the sixteenth valve 35 is connected with a node between the seventeenth valve 26 and the third filter 30, the eighteenth valve 36 is connected at the oxygen pipeline 8, the eighteenth valve 36 is connected with a node between the seventeenth valve 26 and the third filter 30, the thirty-ninth valve 37 is connected at the product outlet pipeline 9, the other end of the thirty-ninth valve 37 is connected with the thirty-seventh valve 14, the temperature control system comprises a first plate heat exchanger 38, a second plate heat exchanger 39 and a jacket 40 fixedly arranged outside the tank body 1, the outlet end of the first plate heat exchanger 38 is connected with the inlet end of the second plate heat exchanger 39, a circulating pump 41 is connected between the outlet end of the second plate heat exchanger 39 and the jacket 40, and a thirty-fifth valve 42 and a softened water pipeline 43 are sequentially connected at a node between the circulating pump 41 and the second plate heat exchanger 39; the inoculation and PH regulation pipeline comprises a peristaltic pump 44, an inoculation bag 45 and an alkali liquor storage tank 46, wherein a liquid pumping port of the peristaltic pump 44 is connected with two stop valves in parallel, one stop valve is connected with the inoculation bag 45, the other stop valve is connected with the alkali liquor storage tank 46, a liquid discharge port of the peristaltic pump 44 is connected with a twenty-seventh valve 47, and the other end of the twenty-seventh valve 47 is fixedly arranged at the top of the tank body 1; the bottom of the tank body 1 is provided with a stirrer 48, an output shaft of the stirrer 48 is connected with a blade 49 extending into the tank body 1, the bottom of the tank body 1 is provided with a temperature sensor 50, and the top of the tank body 1 is provided with a pressure sensor 51.

A PH probe 53 and an oxygen probe 54 are arranged on the side wall of the tank body 1. The device also comprises a sewage discharge pipeline 55, and the sewage discharge ports of the tail gas filter 11, the first filter 27, the second filter 29 and the third filter 30 are all connected with the sewage discharge pipeline 55. A fourteenth valve 56 is connected in parallel to the thirteenth valve 25. The node between the twenty-fifth valve 19 and the twenty-sixth valve 20 is connected to the outlet of the twenty-seventh valve 47. A 40 th valve 57 is connected at a node between the thirty-seventh valve 14 and the thirty-ninth valve 37.

The working process of the utility model is as follows:

s1, tank leakage test, wherein the thirteenth valve 25 is opened, all valves directly connected with the tank 1 are closed, pure compressed air in the pure compressed air pipeline 5 sequentially enters the tank 1 through the thirteenth valve 25 and the second filter 29, the pressure value in the tank 1 is displayed on the pressure sensor 51, and if the pressure value in the tank 1 reaches the set pressure, the tank 1 is not leaked;

s2, preheating the tank body, opening a thirty-fifth valve 42 and a circulating pump 41, pumping softened water in a softened water pipeline 43 into a jacket 40 by the circulating pump 41, simultaneously opening a first plate heat exchanger 38 and a second plate heat exchanger 39, introducing industrial steam into the first plate heat exchanger 38, sequentially passing the industrial steam through the second plate heat exchanger 39 and the circulating pump 41 into the jacket 40, preheating the softened water in the jacket 40 by the industrial steam, raising the temperature in the tank body 1 by the heated softened water, detecting the temperature in the tank body 1 by a temperature sensor 50, and closing the first plate heat exchanger 38 after preheating for a period of time;

s3, sterilizing the tank body, namely opening a twelfth valve 34, an eighth valve 28, a twentieth valve 17, a forty-fourth valve 32, a twentieth valve 31 and a third valve 10, allowing pure steam in a pure steam pipeline 6 to sequentially enter the tank body 1 through the twelfth valve 34, the eighth valve 28, the twenty-first valve 16 and the twentieth valve 17 so as to sterilize the tank body 1, the first filter 27, the second filter 29 and the third filter 30 at high temperature, allowing cold air discharged from the tank body 1 to sequentially enter an exhaust pipeline 3 through a tail gas filter 11 and the third valve 10 in the sterilization process, and sterilizing the tank body 1 after a period of time;

s4, maintaining pressure and cooling of the tank body, opening an eleventh valve 24 and a thirteenth valve 25, enabling a part of pure compressed air in a pure compressed air line 5 to sequentially enter the tank body 1 through the eleventh valve 24, a first filter 27, an eighth valve 28, a twenty-first valve 16 and a twenty-third valve 17, enabling the other part of pure compressed air to enter the tank body 1 through the thirteenth valve 25 and a second filter 29, cooling the tank body 1 through two pure compressed air streams, closing the eleventh valve 24 and the thirteenth valve 25 after the tank body 1 is cooled to a certain temperature, and then adjusting the first valve 12, the third valve 10 and the second valve 13 to adjust the pressure in the tail gas filter 11 so as to adjust the pressure in the tank body 1 and keep the pressure in the tank body 1 unchanged;

s5, receiving the culture medium, opening the stirrer 48, driving the blade 49 to rotate by the stirrer 48, opening the sixth valve 23, allowing the culture medium in the culture medium pipeline 2 to sequentially enter the tank body 1 through the sixth valve 23, the twenty-first valve 16 and the twenty-third valve 17, and allowing the blade 49 to stir the culture medium entering the tank body 1; the addition amount of the culture medium is monitored in real time by the weighing sensor 52, and the rotation speed of the stirrer 48 is adjusted by the addition amount;

s6, cleaning the culture medium pipeline, opening the seventh valve 15, enabling cleaning liquid in the cleaning pipeline 4 to sequentially enter the culture medium pipeline 2 through the seventh valve 15 and the sixth valve 23 to clean the culture medium pipeline 2, and completing cleaning of the culture medium pipeline after cleaning for a period of time so as to prepare for next production;

s7, adjusting PH, opening a peristaltic pump 44, a twenty-seventh valve 47 and stop valves on an alkali liquor storage tank 46, pumping out alkali liquor in the alkali liquor storage tank 46 by the peristaltic pump 44, sequentially feeding the pumped alkali liquor into the tank body 1 through the peristaltic pump 44 and the twenty-seventh valve 47, mixing the alkali liquor with a culture medium under the stirring of a blade 49, monitoring the PH of a mixed liquor in real time through a PH probe 53 in the mixing process, and closing the peristaltic pump 44 after a set value is reached, thereby realizing the online adjustment of the PH; in addition, carbon dioxide can be introduced into the culture medium to adjust the pH of the culture medium, namely, the sixteenth valve 35, the twentieth valve 31, the forty-fourth valve 32 and the deep-layer ventilation ring 33 are opened, and the carbon dioxide in the carbon dioxide pipeline 7 enters the culture medium through the sixteenth valve 35, the twentieth valve 31, the forty-fourth valve 32 and the deep-layer ventilation ring 33 in sequence, so that the online adjustment of the pH is realized; therefore, the reactor has two modes for regulating PH, has more selectivity, can be carried out in another regulating mode when one regulating mode is blocked, ensures the smooth operation of cell culture, and has the characteristic of higher universality;

s8, inoculating, closing a stop valve on the alkali liquor storage tank 46, opening a stop valve and a peristaltic pump 44 on an inoculation bag 45, pumping out the seed liquid in the inoculation bag 45 by the peristaltic pump 44, sequentially feeding the pumped seed liquid into the tank body 1 through the peristaltic pump 44 and a twenty-seventh valve 47, mixing the seed liquid with the mixed liquid in the step S7 under the stirring of a blade 49, adding a certain amount of seed liquid, and closing the stop valves on the peristaltic pump 44 and the inoculation bag 45, so that the inoculation is realized; wherein the seed liquid is animal cell suspension;

s9, culturing the virus antigen, and changing the stirring speed of the stirrer 48 according to the displayed numerical value and the numerical value displayed by the weighing sensor 52; opening the eighteenth valve 36, the twentieth valve 31 and the forty-fourth valve 32, and allowing oxygen in the oxygen pipeline 8 to enter the tank body 1 through the eighteenth valve 36, the twentieth valve 31, the forty-fourth valve 32 and the deep ventilation ring 33 in sequence to provide oxygen required for growth for the mixed liquor, and monitoring the oxygen content in the mixed liquor in real time through the oxygen probe 54; opening an eleventh valve 24, an eighth valve 28 and a twentieth valve 17, and allowing pure compressed air in the pure compressed air line 5 to enter the tank 1 through the eleventh valve 24, the first filter 27, the eighth valve 28, the twenty-first valve 16 and the twentieth valve 17 in sequence to provide pure compressed air required by growth for the mixed liquor; the pressure value in the tank body 1 is changed by changing the throttling sizes of the first valve 12 and the third valve 10, so that the mixed solution grows in a set environment, and after a period of culture period, the required virus antigen can be cultured in the tank body 1; therefore, the reactor can culture the virus antigen in the 2000-10000L large-volume tank body 1, and can improve the suitable growth and metabolism environment for cells, including pH value, oxygen content, stirring speed, pressure and the like.

S10, taking out the finished product virus antigen, and opening the thirty-seventh valve 14 and the thirty-ninth valve 37 to discharge the virus antigen prepared in the tank body 1 into a lower pipeline;

s11, after liquid discharging is finished, opening the seventh valve 15, the twenty-fourth valve 18, the twenty-fifth valve 19, the thirty-seventh valve 14 and the 40 th valve 57, spraying the cleaning liquid in the cleaning pipeline 4 downwards from the spraying ball 22 sequentially through the seventh valve 15, the twenty-first valve 16, the twenty-third valve 17, the twenty-fourth valve 18 and the twenty-fifth valve 19, applying the sprayed cleaning liquid to the inner wall of the tank body 1 to flush away residues on the inner wall of the tank body 1, and discharging the waste liquid to the outside of the tank body 1 sequentially from the thirty-seventh valve 14 and the 40 th valve 57 under the gravity, so that the cleaning of the liquid discharging pipeline is realized.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A reactor for large-scale production of suspension cells is characterized in that: it includes jar body (1), culture medium pipeline (2), inoculation and PH adjusting line, exhaust pipe line (3), washs pipeline (4), pure compressed air pipeline (5), pure steam pipeline (6), carbon dioxide pipeline (7), oxygen pipeline (8), product export pipeline (9) and temperature control system, be connected with third valve (10) and tail gas filter (11) in order between exhaust pipe line (3) and the jar body (1), the both ends of third valve (10) are parallelly connected to have first valve (12) and second valve (13), first valve (12) link to each other with second valve (13), wash pipeline (4) including set firmly in the thirty seventh valve (14) of jar body (1) bottom, wash and be connected with seventh valve (15) in order between pipeline (4) and the thirty seventh valve (14), twenty first valve (16), A twenty-third valve (17), a twenty-fourth valve (18), a twenty-fifth valve (19), a twenty-sixth valve (20) and a thirty-eighth valve (21), wherein the twenty-third valve (17), the twenty-fourth valve (18) and the twenty-fifth valve (19) are all communicated with the top of the tank body (1), the bottoms of the twenty-fourth valve (18) and the twenty-fifth valve (19) are all connected with a spray ball (22) extending into the tank body (1), the culture medium pipeline (2) is connected with a sixth valve (23), the sixth valve (23) is connected with a pure node between the seventh valve (15) and the twenty-first valve (16), the eleventh valve (24), the thirteenth valve (25) and the seventeenth valve (26) are connected in parallel on the compressed air pipeline (5), and the other end of the eleventh valve (24) is sequentially connected with a first filter (27) and an eighth valve (28), the eighth valve (28) is connected to a node between the seventh valve (15) and the twenty-first valve (16), a second filter (29) is connected between the other end of the thirteenth valve (25) and the tank body (1), the other end of the seventeenth valve (26) is sequentially connected with a third filter (30), a twenty-second valve (31) and a forty-fourth valve (32), the other end of the forty-fourth valve (32) is connected with a deep ventilation ring (33), the deep ventilation ring (33) extends into the tank body (1) and is arranged at the lower end part of the tank body (1), the twenty-second valve (31) is connected with the twenty-first valve (16), the twelfth valve (34) is connected to the pure steam pipeline (6), the other end of the twelfth valve (34) is connected to a node between the first filter (27) and the eleventh valve (24), and the sixteenth valve (35) is connected to the carbon dioxide pipeline (7), a sixteenth valve (35) is connected to a node between the seventeenth valve (26) and the third filter (30), an eighteenth valve (36) is connected to the oxygen pipeline (8), the eighteenth valve (36) is connected to a node between the seventeenth valve (26) and the third filter (30), a thirty-ninth valve (37) is connected to the product outlet pipeline (9), the other end of the thirty-ninth valve (37) is connected to the thirty-seventh valve (14), the temperature control system comprises a first plate heat exchanger (38), a second plate heat exchanger (39) and a jacket (40) fixedly arranged outside the tank body (1), the outlet end of the first plate heat exchanger (38) is connected to the inlet end of the second plate heat exchanger (39), a circulating pump (41) is connected between the outlet end of the second plate heat exchanger (39) and the jacket (40), and a thirty-fifth valve(s) (40) is sequentially connected to a node between the circulating pump (41) and the second plate heat exchanger (39) 42) And a softened water line (43); the inoculation and PH regulation pipeline comprises a peristaltic pump (44), an inoculation bag (45) and an alkali liquor storage tank (46), two stop valves are connected in parallel at a liquid pumping port of the peristaltic pump (44), one stop valve is connected with the inoculation bag (45), the other stop valve is connected with the alkali liquor storage tank (46), a twenty-seventh valve (47) is connected at a liquid discharging port of the peristaltic pump (44), and the other end of the twenty-seventh valve (47) is fixedly arranged at the top of the tank body (1); the bottom of the tank body (1) is provided with a stirrer (48), an output shaft of the stirrer (48) is connected with a blade (49) extending into the tank body (1), the bottom of the tank body (1) is provided with a temperature sensor (50), and the top of the tank body (1) is provided with a pressure sensor (51).

2. The reactor for large-scale production of suspension cells according to claim 1, wherein: and a weighing sensor (52) is arranged on the tank body (1).

3. The reactor for large-scale production of suspension cells according to claim 1, wherein: a PH probe (53) and an oxygen probe (54) are arranged on the side wall of the tank body (1).

4. The reactor for large-scale production of suspension cells according to claim 1, wherein: the tail gas filter device further comprises a sewage discharge pipeline (55), and sewage discharge ports of the tail gas filter (11), the first filter (27), the second filter (29) and the third filter (30) are connected with the sewage discharge pipeline (55).

5. The reactor for large-scale production of suspension cells according to claim 1, wherein: a fourteenth valve (56) is connected in parallel to the thirteenth valve (25).

6. The reactor for large-scale production of suspension cells according to claim 1, wherein: and a node between the twenty-fifth valve (19) and the twenty-sixth valve (20) is connected with an outlet of the twenty-seventh valve (47).

7. The reactor for large-scale production of suspension cells according to claim 1, wherein: a 40 th valve (57) is connected at a node between the thirty-seventh valve (14) and the thirty-ninth valve (37).

8. The reactor for large-scale production of suspension cells according to claim 1, wherein: the volume of the tank body (1) is 2000-10000L.