CN213878021U - Mass spectrum device for analyzing lipid metabolites of human brain glioblastoma - Google Patents
Mass spectrum device for analyzing lipid metabolites of human brain glioblastoma Download PDFInfo
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- CN213878021U CN213878021U CN202120132883.7U CN202120132883U CN213878021U CN 213878021 U CN213878021 U CN 213878021U CN 202120132883 U CN202120132883 U CN 202120132883U CN 213878021 U CN213878021 U CN 213878021U
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Abstract
The utility model discloses a mass spectrum device for analyzing lipid metabolites of human glioblastoma, which comprises a placing rack, wherein one end of the placing rack is provided with a bipolar electric coagulation forceps bracket, bipolar electric coagulation forceps are arranged on the bipolar electric coagulation forceps bracket, and the bipolar electric coagulation forceps are connected with a high-frequency electric knife power supply output instrument; the middle part of the placing rack is provided with an operating platform, the other end of the placing rack is provided with a high-resolution time-of-flight mass spectrometer, an ion transmission device is arranged at the inlet end of the high-resolution time-of-flight mass spectrometer, the ion transmission device is also respectively connected with a vacuum pump and a mass spectrum interface, a three-way pipe is arranged at the sample inlet end of the mass spectrum interface, and a heating filament is arranged inside the sample outlet side of the mass spectrum interface; and the other two pipe orifices of the three-way pipe are also respectively connected with an ion transmission pipe and a solvent matrix transmission pipe. The utility model discloses can shorten whole differentiation time and convenient operation's characteristics.
Description
Technical Field
The utility model relates to a mass spectrometer, in particular to a mass spectrometer for analyzing the lipid metabolite of human brain glioblastoma.
Background
Glioblastoma, also known as Glioblastoma Multiforme (GBM), is a malignant grade IV tumor that is biologically aggressive, occurs mainly in the brain, and is the most malignant glioma among astrocytic tumors. GBM is composed mainly of astrocytes and represents about 15% of primary brain tumors. The main treatment mode of GBM is tumor resection, which aims to remove the diseased brain tumor tissue as much as possible, but the normal nerve function of the surrounding brain tissue is very easily damaged due to unclear tumor tissue boundaries during the operation. The brain tumor tissue excised by the operation needs to be made into a tissue section, placed on a glass slide, and after better contrast is presented by H & E staining, histological identification is carried out according to the cell structure under a microscope. Histological identification is the gold standard for tumor identification. However, the process of making tissue slices required for histopathological examination is complex and tedious, a large amount of reagent solvents are required, the whole process is time-consuming, generally, the examination of a tissue sample is completed for about 2 to 3 days, the improvement of the operation precision of brain tumor resection is seriously retarded, and the identification result cannot be provided in time in the operation. Therefore, the prior art has the problems of long time consumption of the whole authentication process and inconvenient operation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a mass spectrum device that is used for human brain glioblastoma lipid metabolite to analyze. The utility model discloses can shorten whole differentiation time and convenient operation's characteristics.
The technical scheme of the utility model: a mass spectrum device for analyzing lipid metabolites of human glioblastoma multiforme comprises a placing rack, wherein one end of the placing rack is provided with a bipolar electric coagulation forceps bracket, bipolar electric coagulation forceps are arranged on the bipolar electric coagulation forceps bracket, and the bipolar electric coagulation forceps are connected with a high-frequency electric knife power supply output instrument; the middle part of the placing rack is provided with an operating platform, the other end of the placing rack is provided with a high-resolution time-of-flight mass spectrometer, an ion transmission device is arranged at the inlet end of the high-resolution time-of-flight mass spectrometer, the ion transmission device is also respectively connected with a vacuum pump and a mass spectrum interface, the feed end of the mass spectrum interface is provided with a three-way pipe, and a heating filament is arranged inside the sample outlet side of the mass spectrum interface; and the other two pipe orifices of the three-way pipe are also respectively connected with an ion transmission pipe and a solvent matrix transmission pipe.
In the mass spectrometer for analyzing the lipid metabolites of the human glioblastoma multiforme, the bipolar coagulation forceps bracket comprises a bracket body, forceps flap insertion and release pipes are arranged on the front side and the rear side of the bracket body, a disinfectant is arranged in the forceps flap insertion and release pipes, and a liquid discharge port is arranged at the bottom of the forceps flap insertion and release pipes.
In the mass spectrometer for analyzing the lipid metabolites of the human glioblastoma multiforme, the feeding end of the ion transmission tube is provided with a conical flaring.
In the mass spectrometer for analyzing the lipid metabolites of the human glioblastoma multiforme, a tapered necking is arranged inside the three-way pipe positioned at the inlet side of the charged aerosol.
In the mass spectrometer for analyzing the lipid metabolites of the human glioblastoma multiforme, a group of uniformly distributed annular bulges are arranged on the inner wall surface of the outlet side of the three-way pipe.
In the mass spectrum device for analyzing the lipid metabolites of the human glioblastoma multiforme, a bidirectional conical piece is further arranged between the mass spectrum interface and the three-way pipe, and a spiral horizontal rotating blade and a spiral vertical rotating blade are arranged in the middle of the bidirectional conical piece.
Compared with the prior art, the utility model discloses utilize the rack to combine bipolar coagulation tweezers, ion transmission device, ion transmission pipe and high-resolution time of flight mass spectrograph etc. to be in the same place, high-resolution time of flight mass spectrograph produces electrified aerosol when gathering bipolar coagulation tweezers to the tissue sample operation through gathering the bipolar coagulation, just can gather the mass spectrum map of lipid metabolite in the tissue sample, and the follow-up researcher of being convenient for is quick distinguishes the tumour tissue, and whole operation process is simple and convenient, can be very big shorten whole differentiation required time. Meanwhile, the charged aerosol is mixed with the solvent matrix for assisting ionization by arranging the three-way pipe, so that the signal intensity of lipid metabolites in brain tissues in a rapid evaporation ionization mass spectrum can be improved; the utility model discloses an inside at the mass spectrum interface sets up heating filament to pollution removal and improvement mass spectrum signal intensity. In addition, the utility model is also provided with the bipolar coagulation forceps bracket for placing the bipolar coagulation forceps and can also finish the disinfection of the bipolar coagulation forceps; the end part of the ion transmission tube is provided with the conical flaring, so that the suction amount of the charged aerosol can be effectively improved; the utility model improves the mixing uniformity of the charged aerosol and the solvent matrix by arranging the tapered necking at the charged aerosol inlet side of the three-way pipe and controlling the speed of the charged aerosol; the utility model discloses it is protruding still through setting up the annular on three-way pipe outlet side inner wall, set up two-way cone and spiral horizontal rotation blade and spiral vertical rotating blade between three-way pipe and mass spectrum interface to this further improvement mixing degree between them, and then improve mass spectrum signal intensity. To sum up, the utility model discloses can shorten whole differentiation time and convenient operation's characteristics.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a partially enlarged view of fig. 1.
The labels in the figures are: the system comprises a 1-placing rack, a 2-bipolar coagulation forceps bracket, a 3-bipolar coagulation forceps, a 4-high-frequency electrotome power output instrument, a 5-operating console, a 6-high-resolution time-of-flight mass spectrometer, a 7-ion transmission device, an 8-vacuum pump, a 9-mass spectrum interface, a 10-three-way pipe, a 10-heating filament, a 12-ion transmission pipe, a 13-solvent matrix transmission pipe, a 14-conical flaring, a 15-conical necking, a 16-annular bulge, a 17-bidirectional conical piece, 18-spiral horizontal rotating blades, 19-spiral vertical rotating blades, a 201-bracket body and a 202-forceps valve insertion tube.
Detailed Description
The following description is made with reference to the accompanying drawings and examples, but not to be construed as limiting the invention.
Examples are given. A mass spectrum device for analyzing lipid metabolites of human glioblastoma multiforme is shown in figure 1 and comprises a placing frame 1, wherein one end of the placing frame 1 is provided with a bipolar coagulation forceps bracket 2, the bipolar coagulation forceps bracket 2 is provided with bipolar coagulation forceps 3, and the bipolar coagulation forceps 3 are connected with a high-frequency electrotome power supply output instrument 4; an operation table 5 is arranged in the middle of the placing rack 1, a high-resolution time-of-flight mass spectrometer 6 is arranged at the other end of the placing rack 1, an ion transmission device 7 is arranged at the inlet end of the high-resolution time-of-flight mass spectrometer 6, the ion transmission device 7 is further connected with a vacuum pump 8 and a mass spectrum interface 9 respectively, a three-way pipe 10 is arranged at the feed end of the mass spectrum interface 9, and a heating filament 11 is arranged inside the discharge side of the mass spectrum interface 9; the other two pipe orifices of the three-way pipe 10 are also respectively connected with an ion transfer pipe 12 and a solvent matrix transfer pipe 13.
The bipolar electric coagulation forceps support 2 comprises a support body 201, forceps valve insertion and release pipes 202 are arranged on the front side and the rear side of the support body 201, disinfectant is arranged in the forceps valve insertion and release pipes 202, and a liquid discharge port is formed in the bottom of the forceps valve insertion and release pipes 202.
The feed end of ion transfer tube 12 is provided with a conical flare 14.
A conical reducing opening 15 is arranged in the three-way pipe 10 positioned at the inlet side of the charged aerosol.
A group of uniformly distributed annular bulges 16 are arranged on the inner wall surface of the outlet side of the three-way pipe 10.
A bidirectional conical piece 17 is further arranged between the mass spectrum interface 9 and the three-way pipe 10, and a spiral horizontal rotating blade 18 and a spiral vertical rotating blade 19 are arranged in the middle of the bidirectional conical piece 17.
The bipolar coagulation forceps are controlled by a high-frequency electrotome power supply output instrument (SJ350B, China Beijing Oriental Shenjian medical instrument Co., Ltd.) and are coupled with a high-resolution time-of-flight mass spectrometer (XevoG2-XS, Vortex, UK) through a special mass spectrum interface.
The resulting charged aerosol was analyzed by mass spectrometry using a teflon PTFE ion transfer tube (1/16"o.d. × 1.0mm i.d.) to introduce the dotted lipid metabolite ions into the mass spectrometer. The introduction of the charged aerosol is driven by the foreline vacuum of the mass spectrometer (ion transport, ion entry into the mass spectrometer), the vacuum of which should be kept around 1.59 mbar. To further increase the mass spectral signal intensity of lipid metabolites, a solvent matrix (organic solvent) is mixed with the charged aerosol sample produced by the brain tissue electrocoagulation process via a tee (T-junction).
At 0.1mLmin-10.2ngmL of organic solvent base mixed with charged aerosol at flow rate-1For the leucine enkephalin, lock mass correction was performed.
Meanwhile, within the mass interface, a Corterra (Kanthalo A1) heating filament (1.2 Ω) that maintains the temperature at 700 deg.C (3V, DC) was placed at the exit of the mass interface to remove contamination and increase the mass signal intensity.
All mass spectrum data are collected in a negative ion ionization mode, and the high-resolution time-of-flight mass spectrometer operates in a sensitivity mode. The mass spectrum scanning time is set to 1s per scanning for analysis, and the scanning mass range is set to be m/z 100-1000. The compensation voltage for the heating filament was set to 40V. The mass spectrometer used high purity argon (99.999%) as the collision gas for the secondary tandem mass spectrometry (MS/MS). The high resolution time-of-flight mass spectrometer was calibrated with a sodium formate solution before being used daily.
Electrified aerosol sample that brain tissue electricity congeals process production is under the negative pressure, inhaled the ion transmission pipe, when through the toper throat, fluid speed can accelerate, after passing the toper throat, along with the volume grow, electrified aerosol speed can descend suddenly, and then can with solvent matrix intensive mixing, after both mix, through annular bellied vortex, the acceleration and deceleration effect of two-way conical member and spiral horizontal rotation blade and spiral vertical rotation blade's effect in proper order for electrified aerosol and solvent matrix intensive mixing.
Claims (6)
1. A mass spectrometry apparatus for lipid metabolite analysis of human brain glioblastoma, characterized by: the electric coagulation forceps comprise a placing frame (1), wherein one end of the placing frame (1) is provided with a bipolar electric coagulation forceps bracket (2), the bipolar electric coagulation forceps bracket (2) is provided with bipolar electric coagulation forceps (3), and the bipolar electric coagulation forceps (3) are connected with a high-frequency electrotome power supply output instrument (4); an operation table (5) is arranged in the middle of the placing frame (1), a high-resolution time-of-flight mass spectrometer (6) is arranged at the other end of the placing frame (1), an ion transmission device (7) is arranged at the inlet end of the high-resolution time-of-flight mass spectrometer (6), the ion transmission device (7) is also connected with a vacuum pump (8) and a mass spectrum interface (9) respectively, a three-way pipe (10) is arranged at the feed end of the mass spectrum interface (9), and a heating filament (11) is arranged inside the sample outlet side of the mass spectrum interface (9); the other two pipe orifices of the three-way pipe (10) are also respectively connected with an ion transmission pipe (12) and a solvent matrix transmission pipe (13).
2. The mass spectrometry apparatus for lipid metabolite analysis of human glioblastoma according to claim 1, wherein: the bipolar coagulation forceps bracket (2) comprises a bracket body (201), forceps valve insertion and release pipes (202) are arranged on the front side and the rear side of the bracket body (201), disinfectant is arranged in the forceps valve insertion and release pipes (202), and a liquid discharge port is arranged at the bottom of the forceps valve insertion and release pipes (202).
3. The mass spectrometry apparatus for lipid metabolite analysis of human glioblastoma according to claim 1, wherein: the feeding end of the ion transmission tube (12) is provided with a conical flaring opening (14).
4. The mass spectrometry apparatus for lipid metabolite analysis of human glioblastoma according to claim 1, wherein: a conical necking (15) is arranged in the three-way pipe (10) positioned at the inlet side of the charged aerosol.
5. The mass spectrometry apparatus for lipid metabolite analysis of human glioblastoma according to claim 1, wherein: a group of uniformly distributed annular bulges (16) are arranged on the inner wall surface of the outlet side of the three-way pipe (10).
6. The mass spectrometry apparatus for lipid metabolite analysis of human glioblastoma according to claim 1, wherein: and a bidirectional conical piece (17) is further arranged between the mass spectrum interface (9) and the three-way pipe (10), and a spiral horizontal rotating blade (18) and a spiral vertical rotating blade (19) are arranged in the middle of the bidirectional conical piece (17).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120132883.7U CN213878021U (en) | 2021-01-18 | 2021-01-18 | Mass spectrum device for analyzing lipid metabolites of human brain glioblastoma |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120132883.7U CN213878021U (en) | 2021-01-18 | 2021-01-18 | Mass spectrum device for analyzing lipid metabolites of human brain glioblastoma |
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| CN213878021U true CN213878021U (en) | 2021-08-03 |
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| CN202120132883.7U Active CN213878021U (en) | 2021-01-18 | 2021-01-18 | Mass spectrum device for analyzing lipid metabolites of human brain glioblastoma |
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