CN213875705U

CN213875705U - Colloidal gold sample application of sample filter equipment

Info

Publication number: CN213875705U
Application number: CN202022203021.7U
Authority: CN
Inventors: 洪建�
Original assignee: Tianjin Meiruite Medical Technology Co ltd
Current assignee: Tianjin Meiruite Medical Technology Co ltd
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2021-08-03
Anticipated expiration: 2030-09-30

Abstract

The utility model belongs to the biological detection field, concretely relates to colloidal gold sample application of sample filter equipment. The utility model provides a colloidal gold sample sampling and filtering device, which comprises a filter and a reaction box, wherein the filter contains protein-coated microspheres, and the bottom of the filter is provided with a thread device; the reaction box is used for placing the colloidal gold detection card, the box cover is provided with a screw device which can be connected with the filter, and the position of the sample pad of the colloidal gold detection card is right below the interface. The utility model discloses can clear away the unnecessary interference among the sample especially serum sample, reduce the serum viscosity, be favorable to improving the sensitivity that colloidal gold detected.

Description

Colloidal gold sample application of sample filter equipment

Technical Field

The utility model belongs to the biological detection field, concretely relates to colloidal gold sample application of sample filter equipment.

Background

Colloidal gold is a common labeling technique, and is a novel immunolabeling technique which uses colloidal gold as a tracer marker to apply to antigen and antibody, has unique advantages, and is widely used in various biological researches. There are two methods most commonly used for colloidal gold in medical testing: colloidal gold immuno-dot filtration and colloidal gold immuno-chromatography.

The colloidal gold immunochromatography method is that a specific antigen or antibody is fixed on a membrane in a strip shape, a colloidal gold labeled reagent (antibody or monoclonal antibody) is adsorbed on a binding pad, when a sample to be detected is added on a sample pad at one end of a detection card, the sample moves forwards through capillary action, the colloidal gold labeled reagent on the binding pad is dissolved and reacts with each other, and then the sample moves to a region of the fixed antigen or antibody, a binding substance of the sample and the gold labeled reagent is specifically bound with the binding substance and is intercepted, and the binding substance is gathered on a detection belt, and a color development result can be observed by naked eyes. The method is very convenient to use.

However, in the detection process, complex samples are often encountered, such as high-viscosity serum or samples with complex components and other substances such as antibody proteins interfering with the detection result, which affects the accuracy and sensitivity of the detection result. For example, human serum and plasma contain albumin, have high viscosity, and are not beneficial to chromatographic diffusion when used for colloidal gold detection; if the interference of albumin can be eliminated by the anti-human albumin antibody, the reaction time can be advantageously shortened. For example, after 2019-nCoV virus invades a human body, the human body can generate specific antibodies IgM antibody and IgG antibody, wherein IgM antibody positive indicates recent infection, and IgG positive indicates that the disease state enters a recovery period or existing infection exists. However, mutual interference of IgM/IgG antibodies often exists in the detection process, and if one of the IgM antibodies or the IgG antibodies in the sample can be filtered and removed in advance, the mutual interference of the IgM/IgG antibodies can be eliminated, so that the detection sensitivity and accuracy are improved. There are many examples of such. Therefore, we propose a filtering device for loading colloidal gold sample to solve the above problems.

Disclosure of Invention

In order to solve the defects existing in the prior art, the utility model provides a colloidal gold sample application of sample filter equipment.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a filter device for sample adding of colloidal gold samples comprises a filter and a reaction box. The filter comprises a filter cup body, wherein the cup body contains protein-coated microspheres, and the bottom of the cup body is provided with a thread device; the reaction box is a plastic full-transparent box body, and a screw device is arranged at the box cover.

The technical scheme of the utility model, the microballon of the peridium albumen that holds in the filter cup, microballon surface peridium one or more protein, including but not limited to anti human albumin antibody, anti human IgG antibody, anti human IgM antibody etc. its effect is the material that has the interference to the testing result in adsorbing the sample, clears away the interference, improves the accuracy, the sensitivity of testing result.

In the technical scheme of the utility model, the reaction box is a plastic transparent box body used for placing the colloidal gold detection card, a screw device is arranged at the box cover and can be connected with the filter through screw threads, and the position of the sample pad of the colloidal gold detection card is arranged right below the interface; the reaction condition that the colloidal gold detected can directly be observed to the full transparent box body, and the plastics material is convenient for transport and deposit and with low costs, whole sexual valence relative altitude.

In the technical proposal of the utility model, the filter cup body is provided with a cover to prevent the microspheres from spilling out; a filter screen is arranged at the joint of the cup body and the threaded device, so that microspheres are prevented from leaking from the lower part, and smooth liquid passing can be ensured; the cup body is in full-sealed package before use, so that pollution is prevented.

The technical proposal of the utility model is that the reaction box is packaged in a full-sealing way before use, thus preventing pollution.

The utility model has the advantages that: can eliminate unnecessary interference in samples during colloidal gold immunochromatography detection, reduce the viscosity, improve the accuracy and sensitivity of detection results, and is worthy of popularization.

Drawings

Fig. 1 is the utility model provides a structure diagram of a colloidal gold sample application of sample filter equipment.

Fig. 2 is a schematic diagram of a filter of the colloidal gold sample adding and filtering device provided by the utility model.

Fig. 3 is a schematic view of a reaction box of the colloidal gold sample adding and filtering device provided by the present invention.

In the figure: 3. a filter cup body; 4. protein-coated microspheres; 5. a bottom threaded means; 6. a screw device is arranged at the box cover of the reaction box; 7. a transparent reaction cartridge body; 8. colloidal gold test card.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and the embodiments described herein are only used for explaining the present invention, and are not used for limiting the scope of the present invention.

As shown in FIG. 1, a filter device for loading a colloidal gold sample includes a filter 1 and a reaction cassette 2. The filter 1 and the reaction box 2 are connected by a screw device.

As shown in fig. 2, the filter 1 comprises a cup body 3 and protein-coated microspheres 4, the bottom of which is provided with a screw means 5. The filter cup body 3 of the filter 1 is provided with a cover to prevent the microspheres 4 from spilling; a filter screen is arranged at the joint of the cup body 3 and the thread device 4, so that microspheres are prevented from leaking from the lower part, and smooth liquid passing can be ensured; the cup body is in full-sealed package before use, so that pollution is prevented. When the sample is added, the package and the cup cover are removed, and the sample is directly added into the filter 1 to be fully contacted and reacted with the microspheres 4.

As shown in figure 2, the reaction box 2 is used for placing the colloidal gold detection card, a screw device 6 is arranged at the box cover of a full transparent plastic box body 7 of the reaction box and can be connected with the filter 1 through screw threads, and the position of a sample pad of the colloidal gold detection card is arranged below the screw device.

This use is novel, during the use: firstly, placing a colloidal gold detection card 8 in a reaction box 2, and placing a sample pad position of the detection card right below the reaction box 2; then the filter 1 and the reaction box 2 are screwed up and connected with a screw device 6 through a screw device 5; the package and the cup cover of the filter 1 are removed after connection is ensured, a sample is directly added into the filter cup body 3 to be fully contacted and reacted with the microspheres 4, the filtered sample has the interferents removed, and the filtered sample directly flows into the sample pad of the colloidal gold detection card 8 in the reaction box 2 through the thread to be detected.

The above description is only a preferred embodiment of the present invention, but the scope of the present invention is obviously not limited thereto. Without departing from the principle of the present invention, a person skilled in the art can make equivalent changes or substitutions to the related technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. The utility model provides a colloidal gold sample application of sample filter equipment which characterized in that: the device includes filter (1) and reaction box (2), contain peridium albumen microballon (4) in the filter, there is threaded device (5) bottom, the reaction box is used for placing colloidal gold detection card, and lid department has screw device (6), can pass through threaded connection with filter (1), and the kneck below is the sample pad position of colloidal gold detection card.

2. The device for filtering a sample of colloidal gold according to claim 1, wherein: the microsphere coated protein (4) can be one or more proteins which are used for eliminating substances which interfere with the detection result in the sample.

3. The device for filtering a sample of colloidal gold according to claim 1, wherein: the reaction box (2) is a fully transparent plastic box body (7), so that the reaction condition of the colloidal gold detection card can be observed conveniently, and the transportation and storage are facilitated.