CN213866164U - Device for extracting total RNA of bee annex - Google Patents
Device for extracting total RNA of bee annex Download PDFInfo
- Publication number
- CN213866164U CN213866164U CN202021933054.0U CN202021933054U CN213866164U CN 213866164 U CN213866164 U CN 213866164U CN 202021933054 U CN202021933054 U CN 202021933054U CN 213866164 U CN213866164 U CN 213866164U
- Authority
- CN
- China
- Prior art keywords
- upper panel
- frame
- bee
- total rna
- clamping plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 239000002184 metal Substances 0.000 claims abstract description 5
- 238000005057 refrigeration Methods 0.000 claims description 12
- 239000004065 semiconductor Substances 0.000 claims description 10
- 238000010030 laminating Methods 0.000 claims description 2
- 241000256844 Apis mellifera Species 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000004570 mortar (masonry) Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000008786 sensory perception of smell Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a draw device of honeybee annex total RNA, include: a liquid containing tank made of metal at the bottom and having an open top; the frame is detachably arranged in the liquid containing box; the upper panel is detachably arranged in the frame, and is provided with a through groove and a plurality of circular through holes, and the through groove is communicated with the circular through holes; the clamping plate is inserted into the through groove in the upper panel, and a plurality of waist-shaped holes are formed in the clamping plate; the clamping plate is connected with the upper panel through the locking mechanism, and the clamping plate can be fixed in the through groove in the upper panel after the locking mechanism is screwed down; the lower panel is detachably arranged in the frame. The utility model discloses in, can fix the centrifuging tube of equidimension not, easy and simple to handle, and prevent effectively that the RNA among the experimental sample from taking place to degrade at the grinding in-process, improve device's practicality.
Description
Technical Field
The utility model belongs to the technical field of the RNA extraction technique and specifically relates to an extract device of honeybee annex total RNA.
Background
The acquisition of high-quality RNA is an important basis for the research of bee molecular biology, the high-quality RNA provides a reliable experimental sample for the subsequent research of bee development and bee olfaction determination mechanisms and other related contents by adopting a high-throughput sequencing technology, and plays a positive role in the research of bee biology. The extraction device that the laboratory is commonly used has mortar, handheld electronic mortar ware, high flux tissue grinder, and the mortar cooperation liquid nitrogen can be broken bee annex mortar, and the required experimental sample of shortcoming is more, need spend the plenty of time dissects the honeybee, and the liquid nitrogen splashes easily in the operation process and causes the sample loss, and the required cost of the two kinds of devices of back is higher.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the technical problem and providing a device for extracting total RNA of bee appendages.
The technical scheme of the utility model: an apparatus for extracting total RNA of a bee adjunct, comprising:
a liquid containing tank made of metal at the bottom and having an open top;
the frame is detachably arranged in the liquid containing box;
the upper panel is detachably arranged in the frame, a through groove and a plurality of circular through holes are formed in the upper panel, and the through groove is communicated with the circular through holes;
the clamping plate is inserted into the through groove in the upper panel, and a plurality of waist-shaped holes are formed in the clamping plate;
the clamping plate is connected with the upper panel through the locking mechanism, and the clamping plate can be fixed in the through groove in the upper panel after the locking mechanism is screwed down;
and the lower panel is detachably arranged in the frame.
Preferably, the frame is connected with the liquid containing box in a sliding fit mode.
Preferably, the frame is U-shaped, and a plurality of horizontal chutes are oppositely arranged on the inner walls of two sides of the frame, and the upper panel and the lower panel are in sliding fit with the chutes.
Preferably, the locking mechanism comprises a screw, and the screw penetrates through the clamping plate and is in threaded connection with the upper panel.
Preferably, the refrigeration device is further included and is used for refrigerating the normal-temperature liquid.
Preferably, the refrigerating device comprises a semiconductor refrigerating piece, and the refrigerating end of the semiconductor refrigerating piece is attached to the bottom of the liquid containing box.
Preferably, the bottom of the liquid containing tank is provided with a support.
Preferably, the width of the frame and the lower panel is smaller than the width of the inside of the liquid containing tank.
The utility model has the advantages that: in the utility model, the clamping plate can move in the upper panel, centrifuge tubes with different sizes can be fixed, the distance between the upper panel and the lower panel can be adjusted, so that the device can fix centrifuge tubes with different lengths, the operation is simple and convenient, and the refrigerating device can refrigerate normal temperature liquid, so that the sample is in a low temperature state in the centrifuge tube, and the RNA in the experimental sample is effectively prevented from being degraded in the grinding process; the device has the advantages of simple structure, low cost and strong practicability.
Drawings
FIG. 1 is a cross-sectional view of the overall structure of the preferred embodiment of the present invention;
FIG. 2 is a top view of the liquid holding tank in connection with the frame in the preferred embodiment of the present invention;
FIG. 3 is a top view of the upper deck panel in the preferred embodiment of the present invention;
FIG. 4 is a front view of the upper panel in the preferred embodiment of the present invention;
FIG. 5 is a top view of a clamping plate in the preferred embodiment of the present invention;
FIG. 6 is a top view of the connection between the upper panel and the clamping plate in the preferred embodiment of the present invention;
fig. 7 is a partially enlarged view of fig. 1A.
Reference numerals: liquid holds case 1, vertical spout 11, support 12, frame 2, protruding portion 21, horizontal spout 22, upper panel 3, logical groove 31, circular through-hole 32, cardboard 4, kidney-shaped hole 41, lower panel 5, screw 6, semiconductor refrigeration piece 7.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 to 7, an apparatus for extracting total RNA of honeybee appurtenances, comprising:
a liquid containing tank 1 made of metal at the bottom and having an open top;
the frame 2 is detachably arranged in the liquid containing box 1;
the upper panel 3 is detachably arranged in the frame 2, a through groove 31 and a plurality of circular through holes 32 are arranged on the upper panel 3, and the through groove 31 is communicated with the circular through holes 32;
the clamping plate 4 is inserted into the through groove 31 on the upper panel 3, and a plurality of waist-shaped holes 41 are formed in the clamping plate 4;
the clamping plate 4 is connected with the upper panel 3 through the locking mechanism, and the clamping plate 4 can be fixed in the through groove 31 on the upper panel 3 after the locking mechanism is screwed down;
and the lower layer panel 5 is detachably arranged in the frame 2. The utility model discloses in, pass circular through-hole 32 and waist shape hole 41 and the 5 bottom contacts of lower panel with centrifugal tube bottom, remove 4 backs of cardboard to screw up locking mechanism, the centrifuging tube is fixed between circular through-hole 32 and waist shape hole 41, prevents that the sample centrifuging tube from rocking when the centrifuging tube internal grinding, and adding frozen water or liquid nitrogen in the case 1 is held to past liquid, makes the sample be in the RNA that low temperature state effectively prevented among the experimental sample and takes place the degradation in the grinding process in the centrifuging tube.
As a preferred embodiment of the present invention, it may also have the following additional technical features:
in this embodiment, the frame 2 is connected to the liquid containing tank 1 in a sliding fit manner. Frame 2 is convenient to be taken out from liquid containing box 1, and frame 2 can be stably fixed simultaneously, and frame 2 can not take place to rock when making the grinding.
Specifically, the two sides of the frame 2 are provided with protruding portions 21 extending outwards, the inner walls of the two sides of the liquid containing box 1 are provided with vertical sliding grooves 11, and the protruding portions 21 are connected with the vertical sliding grooves 11 in a sliding fit mode.
In this embodiment, the frame 2 is U-shaped, the inner walls of the two sides of the frame are oppositely provided with a plurality of transverse sliding grooves 22, and the upper panel 3 and the lower panel 5 are in sliding fit with the sliding grooves 22. The upper panel 3 and the lower panel 5 can be freely disassembled on the frame 2, and the distance between the upper panel 3 and the lower panel 5 is adjustable, so that centrifugal tubes with different lengths can be fixed.
In this embodiment, the locking mechanism includes a screw 6, and the screw 6 passes through the clamping plate 4 and is in threaded connection with the upper panel 3. The screw 6 is rotated, and one end of the screw 6 is in threaded connection with the upper panel 3, so that the clamping plate 4 is fixed on the upper panel 3.
In this embodiment, still include refrigerating plant, refrigerating plant is used for giving the normal atmospheric temperature liquid refrigeration. When only the normal temperature liquid exists, the normal temperature liquid inside the liquid containing box 1 is refrigerated.
In this embodiment, refrigerating plant includes semiconductor refrigeration piece 7, and 7 refrigeration ends of semiconductor refrigeration piece hold 1 bottom laminating of case with liquid. Specifically, semiconductor refrigeration piece 7 sets up and holds 1 bottoms in liquid, and liquid holds 1 bottoms in the case and be the metal, produces the heat exchange.
In this embodiment, the liquid containing tank 1 is provided with a support 12 at the bottom. The liquid containing box 1 is far away from the desktop or the ground, so that the heating end of the semiconductor refrigerating sheet 7 can radiate heat conveniently.
In this embodiment, the width of the frame 2 and the lower panel 5 is smaller than the width of the inside of the liquid containing tank 1. The width of the frame 2 is smaller than the width of the inside of the liquid containing box 1, so that the liquid containing box 1 has redundant space for placing the clamping plates 4; the width of lower floor panel 5 is less than the inside width of liquid containing box 1, and the liquid that holds box 1 inside to liquid can not the layering, prevents that semiconductor refrigeration piece 7 can't refrigerate the water on lower floor panel 5 upper portion.
It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. An apparatus for extracting total RNA from bee appendages, comprising:
a liquid containing tank (1) made of metal at the bottom and having an open top;
the frame (2) is detachably arranged in the liquid containing box (1);
the upper panel (3) is detachably arranged in the frame (2), a through groove (31) and a plurality of circular through holes (32) are formed in the upper panel (3), and the through groove (31) is communicated with the circular through holes (32);
the clamping plate (4) is inserted into the through groove (31) in the upper panel (3), and a plurality of waist-shaped holes (41) are formed in the clamping plate (4);
the clamping plate (4) is connected with the upper panel (3) through the locking mechanism, and the clamping plate (4) can be fixed in the through groove (31) on the upper panel (3) after the locking mechanism is screwed down;
and the lower layer panel (5) is detachably arranged in the frame (2).
2. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: the frame (2) is connected with the liquid containing box (1) in a sliding fit manner.
3. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: frame (2) are the U-shaped, and the opposition is provided with a plurality of horizontal spout (22) on its both sides inner wall, upper panel (3) and lower panel (5) with spout (22) sliding fit.
4. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: the locking mechanism comprises a screw (6), and the screw (6) penetrates through the clamping plate (4) to be in threaded connection with the upper panel (3).
5. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: the refrigeration device is used for refrigerating the normal-temperature liquid.
6. The apparatus for extracting total RNA of bee appurtenances according to claim 5, wherein: refrigerating plant includes semiconductor refrigeration piece (7), semiconductor refrigeration piece (7) refrigeration end with liquid holds case (1) bottom laminating.
7. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: the bottom of the liquid containing box (1) is provided with a support (12).
8. The device for extracting total RNA of bee appurtenances according to claim 1, wherein: the width of the frame (2) and the width of the lower layer panel (5) are smaller than the width of the inside of the liquid containing box (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021933054.0U CN213866164U (en) | 2020-09-07 | 2020-09-07 | Device for extracting total RNA of bee annex |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021933054.0U CN213866164U (en) | 2020-09-07 | 2020-09-07 | Device for extracting total RNA of bee annex |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213866164U true CN213866164U (en) | 2021-08-03 |
Family
ID=77047848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021933054.0U Expired - Fee Related CN213866164U (en) | 2020-09-07 | 2020-09-07 | Device for extracting total RNA of bee annex |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213866164U (en) |
-
2020
- 2020-09-07 CN CN202021933054.0U patent/CN213866164U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210803 |