CN213843281U

CN213843281U - Test paper strip for detecting cat HIV by using fluorescence immunochromatography technology

Info

Publication number: CN213843281U
Application number: CN202022022977.7U
Authority: CN
Inventors: 杨挥
Original assignee: Jiaxing Dawnsail Biotechnology Co ltd
Current assignee: Jiaxing Dawnsail Biotechnology Co ltd
Priority date: 2020-09-16
Filing date: 2020-09-16
Publication date: 2021-07-30
Anticipated expiration: 2030-09-16

Abstract

The utility model discloses a detect test paper strip of cat AIDS virus with fluorescence immunochromatography technique relates to cat AIDS virus and detects technical field, including the connecting plate, the stock solution hole has been seted up at the middle part of connecting plate, and the through-hole has all been seted up to the both sides inner wall in stock solution hole, and the filling opening has been seted up to the upper end inner wall in stock solution hole, and the open end of filling opening sets up to leaking hopper-shaped, and a plurality of test paper layers that are equidistant distribution of a side fixedly connected with of connecting plate, the sponge hole has been seted up to test paper layer's one end. The utility model discloses a plurality of examination paper layers of a side fixedly connected with of connecting plate, the spongy hole has been seted up to the upper end on examination paper layer, and the connecting hole corresponding with the spongy hole is seted up to a side of connecting plate, and the inner chamber intercommunication of connecting hole and through-hole, the sample solution of through-hole inner chamber evenly enter into the inner chamber in spongy hole on a plurality of test paper layers to can carry out the multiunit and detect, avoided the detection to appear the contingency and influence the testing result.

Description

Test paper strip for detecting cat HIV by using fluorescence immunochromatography technology

Technical Field

The utility model relates to a cat HIV detects technical field, in particular to with fluorescence immunochromatography detect test paper strip of cat HIV.

Background

Infection with feline aids can cause dogs or cats to become depressed, anorexia, fever, loose stools or vomiting, followed by frequent vomiting and severe diarrhea, with high infection and mortality. If a dog or cat is infected with feline HIV, early detection and early treatment is required. At present, most of the existing test strips for detecting the feline immunodeficiency virus are independently arranged, so that the contingency is easy to occur in the detection process of a sample, and the detection result is influenced.

Therefore, it is necessary to invent a test strip for detecting feline aids virus by using fluorescence immunochromatography to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a detect test paper strip of cat AIDS virus with fluorescence immunochromatography to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a test paper strip for detecting cat HIV by fluorescence immunochromatography comprises a connecting plate, wherein the middle part of the connecting plate is provided with a liquid storage hole, the inner walls of the two sides of the liquid storage hole are provided with through holes, the inner wall of the upper end of the liquid storage hole is provided with a liquid feeding port, the opening end of the liquid filling opening is funnel-shaped, one side surface of the connecting plate is fixedly connected with a plurality of test paper layers which are distributed at equal intervals, one end of the test paper layer is provided with a sponge hole, one side surface of the connecting plate is provided with a connecting hole corresponding to the sponge hole, the inner cavity of the sponge hole is connected with a sponge column in an adhesive way, one end of the sponge column is positioned in the connecting hole, a marker hole is arranged at one end of the sponge hole, a marker pad is arranged in the inner cavity of the marker hole, the multifunctional mark comprises a mark body, and is characterized in that a capturing hole is formed in one end of the mark body hole, a capturing film is arranged in the inner cavity of the capturing hole, and a T line and a C line are respectively arranged on two sides of the middle of the capturing film.

Preferably, an observation hole is formed in the inner wall of the upper end of the capture hole, a transparent film is connected to the middle of the upper surface of the test paper layer in an adhering mode, and the transparent film is located at the opening end of the observation hole.

Preferably, the marker pad contains a cat HIV detection antibody marked by fluorescent microspheres, and the capture membrane contains capture probes.

Preferably, the marker pad is made of a glass fiber material, and the capture membrane is a nitrocellulose membrane.

Preferably, the T-line contains a feline hiv capture antibody and the C-line contains a secondary antibody that binds to a feline hiv detection antibody.

The utility model discloses a technological effect and advantage:

1. the utility model discloses a fixedly connected with a plurality of examination paper layers in a side of connecting plate, the upper end of examination paper layer has seted up the sponge hole, a side of connecting plate has seted up the connecting hole corresponding with the sponge hole, the connecting hole communicates with the inner chamber of through-hole, the sample solution of through-hole inner chamber evenly enters into the inner chamber of the sponge hole on a plurality of test paper layers to can carry out multiunit detection, avoid detecting the appearance and accidentally influencing the testing result;

2. the utility model discloses an observation hole has been seted up at the upper end inner wall of catching the hole, and the upper surface middle part adhesive connection of examination ply has transparent film, and transparent film is located the open end of observation hole, and the observation hole is convenient for observe T line and C line, and transparent film can cover the opening of observation hole to avoid catching bacterium or dust pollution in the membrane received the outside air.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic side-sectional view of the structure of the test paper layer of the present invention.

In the figure: 1. a connecting plate; 2. a liquid storage hole; 3. a through hole; 31. connecting holes; 4. a liquid filling port; 5. a test paper layer; 6. sponge holes; 7. a sponge column; 8. a marker well; 9. a marker pad; 10. a capture well; 11. A capture membrane; 12. a T line; 13. c line; 14. an observation hole; 15. a transparent film.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a test strip for detecting cat HIV by fluorescence immunochromatography as shown in figure 1-2, as shown in fig. 1, including connecting plate 1, stock solution hole 2 has been seted up at the middle part of connecting plate 1, stock solution hole 2 is used for temporary storage sample solution, through-hole 3 has all been seted up to the both sides inner wall in stock solution hole 2, filling opening 4 has been seted up to the upper end inner wall in stock solution hole 2, filling opening 4's open end sets up to leaking hopper-shaped, filling opening 4 is used for the inner chamber dropwise add sample solution to stock solution hole 2, a plurality of test paper layers 5 that are equidistant distribution of a side fixedly connected with of connecting plate 1, sponge hole 6 has been seted up to test paper layer 5's one end, connecting hole 31 corresponding with sponge hole 6 has been seted up to a side of connecting plate 1, the inner chamber adhesive connection in sponge hole 6 has sponge post 7, the one end of sponge post 7 is located connecting hole 31, sponge post 7 can absorb the sample solution of 3 inner chambers of through-hole.

As shown in fig. 2, a marker hole 8 is formed at one end of a sponge hole 6, a marker pad 9 is arranged in an inner cavity of the marker hole 8, the marker pad 9 is made of a glass fiber material, the marker pad 9 contains a cat anti-virus detection antibody marked by fluorescent microspheres, a capture hole 10 is formed at one end of the marker hole 8, a capture film 11 is arranged in an inner cavity of the capture hole 10, the capture film 11 is a nitrocellulose film, namely an NC film, the capture film 11 contains a capture probe, a T line 12 and a C line 13 are respectively arranged at two sides of the middle part of the capture film 11, the T line 12 contains a cat anti-virus capture antibody, the C line 13 contains a secondary antibody capable of combining with the cat anti-virus detection antibody, an observation hole 14 is formed in the inner wall of the upper end of the capture hole 10, a transparent film 15 is adhesively connected to the middle part of the upper surface of the test paper layer 5, the transparent film 15 is located at the open end of the observation hole 14, the observation hole 14 is arranged to facilitate the inspection of the T line 12 and the C line 13, the provision of the transparent film 15 can prevent the trapping film 11 from being contaminated by external bacteria or dust.

The utility model discloses the theory of operation:

when the device is used, a sample solution is dripped into the inner cavity of the liquid storage hole 2 through the liquid filling port 4 until the inner cavity of the through hole 3 is filled, the sample solution in the inner cavity of the through hole 3 is absorbed by the plurality of sponge columns 7, the sample solution in the sponge columns 7 reaches the marker pad 9 firstly under the action of gravity, and the cat HIV in the sample is combined with the cat HIV detection antibody marked by the fluorescent microspheres in the marker pad 9 to form a 'cat HIV-cat HIV detection antibody-fluorescent microspheres' compound;

when the solution continues to move to reach the T line 12 on the capture membrane 11, the 'cat HIV-cat HIV detection antibody-fluorescent microsphere' in the solution and the cat HIV capture antibody form a 'cat HIV capture antibody-cat HIV detection antibody-fluorescent microsphere' compound and are fixed on the T line 12, then a detector is used for detecting a fluorescent signal on the T line 12, the concentration of the cat HIV can be judged according to the signal intensity, and the concentration of the cat HIV is high when the fluorescent signal is strong, so that the device has higher sensitivity compared with a traditional test strip.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a test paper strip with fluorescence immunochromatography detects cat HIV, includes connecting plate (1), its characterized in that: the middle part of the connecting plate (1) is provided with a liquid storage hole (2), the inner walls of two sides of the liquid storage hole (2) are provided with through holes (3), the inner wall of the upper end of the liquid storage hole (2) is provided with a liquid adding opening (4), the opening end of the liquid adding opening (4) is funnel-shaped, one side surface of the connecting plate (1) is fixedly connected with a plurality of test paper layers (5) which are distributed at equal intervals, one end of each test paper layer (5) is provided with a sponge hole (6), one side surface of the connecting plate (1) is provided with a connecting hole (31) corresponding to the sponge hole (6), the inner cavity of each sponge hole (6) is connected with a sponge column (7) in an adhering mode, one end of each sponge column (7) is located in the connecting hole (31), one end of each sponge hole (6) is provided with a marker hole (8), and the inner cavity of each marker hole (8) is provided with a marker pad (9), a capture hole (10) is formed in one end of the marker hole (8), a capture film (11) is arranged in the inner cavity of the capture hole (10), and a T line (12) and a C line (13) are respectively arranged on two sides of the middle of the capture film (11).

2. The test strip for detecting feline immunodeficiency virus (HIV) according to claim 1, wherein: an observation hole (14) is formed in the inner wall of the upper end of the capture hole (10), a transparent film (15) is connected to the middle of the upper surface of the test paper layer (5) in an adhering mode, and the transparent film (15) is located at the opening end of the observation hole (14).

3. The test strip for detecting feline immunodeficiency virus (HIV) according to claim 2, wherein: the marker pad (9) contains a cat HIV detection antibody marked by fluorescent microspheres, and the capture membrane (11) contains a capture probe.

4. The test strip for detecting feline HIV by using fluoroimmunoassay chromatography technique according to claim 3, wherein: the marker pad (9) is made of a glass fiber material, and the capture membrane (11) is a nitrocellulose membrane.

5. The test strip for detecting feline HIV by fluoroimmunoassay according to claim 4, wherein: the T line (12) contains a feline HIV capture antibody, and the C line (13) contains a secondary antibody that binds to a feline HIV detection antibody.