CN213835318U - Automatic coater for yeast library construction experiment - Google Patents
Automatic coater for yeast library construction experiment Download PDFInfo
- Publication number
- CN213835318U CN213835318U CN202022796010.4U CN202022796010U CN213835318U CN 213835318 U CN213835318 U CN 213835318U CN 202022796010 U CN202022796010 U CN 202022796010U CN 213835318 U CN213835318 U CN 213835318U
- Authority
- CN
- China
- Prior art keywords
- connecting rod
- dish cover
- coating brush
- library construction
- yeast library
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 19
- 238000002474 experimental method Methods 0.000 title claims abstract description 18
- 238000010276 construction Methods 0.000 title claims abstract description 13
- 239000011248 coating agent Substances 0.000 claims abstract description 63
- 238000000576 coating method Methods 0.000 claims abstract description 63
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 25
- 239000001963 growth medium Substances 0.000 abstract description 21
- 241000894006 Bacteria Species 0.000 abstract description 7
- 241000233866 Fungi Species 0.000 description 12
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007901 soft capsule Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011049 pearl Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an automatic coating device for yeast library construction experiments, which comprises a cylindrical dish cover, wherein a coating brush is fixed on the inner side of the dish cover and is vertically arranged in the diameter direction of the dish cover; a handle is arranged at the top of the dish cover, a driving motor is arranged in the handle, a vertical connecting rod is arranged on the dish cover, and the driving motor is rotationally connected with the connecting rod through a coupler; the connecting rod movably penetrates through the dish cover and the coating brush, and the connecting rod is fixed with the dish cover through a clamping device. The automatic coater can suck liquid through the connecting rod and sample and then drop into the culture medium, the driving motor in the automatic coater is started to enable the coating brush in the dish cover to rotate through the connecting handle, then the bacterium liquid is uniformly coated on the culture medium, and the operation is carried out in the dish cover to prevent external pollution.
Description
Technical Field
The utility model belongs to the technical field of experimental facilities, in particular to utilize a large amount of glass pearls to combine together to carry out the automatic coating ware of scribbling the board in the experiment is found to the yeast library.
Background
In current biological research, the technology for detecting protein-protein interaction is mainly yeast double hybrid. In the experiment, in order to simultaneously screen many proteins interacting with the target protein, a yeast library containing a large number of cDNA fragments needs to be constructed for screening. When constructing the library, 80-100 culture dishes with the specification of 150mm are prepared, the library bacterial liquid is equally coated on the culture medium with the specification, and the monoclonal antibody with the size of more than 107 is obtained by constant temperature culture, so that the yeast library is successfully constructed. In the step, on one hand, the bacterial liquid needs to be uniformly coated on the flat plate, so that the condition that bacterial colonies grow unevenly on the plate is avoided, and on the other hand, the workload is large, so that the time is saved as much as possible.
At present, the devices used in sheet coating operations are mainly divided into two categories: the first type is a triangular glass coating rod, which has the advantages of convenient taking, no need of sterilization in advance and only need of burning on an alcohol lamp, and has the disadvantages that manual operation is needed during coating, so that time is delayed, and on the other hand, the coating is not uniform due to the manual operation, so that the growth and distribution of bacterial colonies are not uniform, and the result is influenced; the other type is 4mm coated glass beads with the specification, the operation efficiency can be improved, the glass beads can be repeatedly used after sterilization, bacteria liquid can be uniformly distributed on the culture medium by manually shaking the culture medium, and the defect that in an experiment with large workload, mechanical operation is always repeated by manpower, and time and manpower are wasted.
The current coating tools are manually operated, which easily results in uneven coating, and when a plurality of culture mediums need to be coated at the same time, the repeated mechanical operation easily wastes manpower.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an automatic coating ware for experiment is found to yeast library can evenly coat the fungus liquid on the culture medium, improves operating efficiency, and can prevent that the fungus liquid from polluting, still conveniently dismantles disinfection and isolation.
In order to achieve the above purpose, the solution of the present invention is:
an automatic coating device for a yeast library construction experiment comprises a cylindrical dish cover, wherein a coating brush is fixed on the inner side of the dish cover and is vertically arranged in the diameter direction of the dish cover; a handle is arranged at the top of the dish cover, a driving motor is arranged in the handle, a connecting rod is arranged at the center of the dish cover, and the driving motor is rotationally connected with the connecting rod through a coupler; the connecting rod movably penetrates through the dish cover and the coating brush, and the connecting rod is higher than the dish cover; a rotary baffle is arranged at the bottom end of the coating brush and corresponds to the connecting rod, and the connecting rod is fixed with the dish cover through a clamping device;
the connecting rod includes hypomere, middle section and upper segment, the hypomere is transparent hollow tube and the bottom of this hypomere is the bell mouth, and the middle section is the flexible glue and inhales the bag pipe, and the upper segment is ordinary cylinder, the hypomere is connected with the middle section and is link up mutually, the upper segment is through at least two montants and hypomere fixed connection, the space that this montant encloses is arranged in to the middle section.
The dish cover is made of transparent materials.
The rotary baffle is arranged at the bottom end of the coating brush through a rotary spring.
The coating brush is of a square plate structure, and the bottom end of the coating brush is an arc surface; the coating brush is fixed on the inner top surface of the dish cover, and the bottom end of the coating brush is higher than the bottom end of the dish cover.
The handle is provided with a switch for controlling the driving motor.
The clamping device comprises a fixed rod, a locking ring and a locking bolt, wherein the fixed rod, the locking ring and the locking bolt are arranged at the upper end of the dish cover, the locking ring is fixed on the fixed rod, the connecting rod penetrates through the locking ring, and a threaded hole is formed in the locking ring; the locking bolt is matched with the threaded hole to fix the connecting rod in position.
And one end of the lower section of the connecting rod, which is close to the middle section, is provided with a rubber ring, and the locking bolt is propped against and locked on the rubber ring.
The outer wall of the lower section is also provided with a metering scale.
The automatic coating device is also provided with a bottom vessel, and the vessel cover and the bottom vessel are mutually buckled.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:
(1) the utility model discloses a be equipped with the ware lid, be equipped with the coating brush in the ware lid, can be with dripping culture medium that has the fungus liquid to in the ware lid, the bottom and the culture medium surface contact of coating brush, the connecting rod is connected the handle and is started the driving motor in the handle, makes the ware lid and the coating brush rotation in it, then can evenly coat the fungus liquid on the culture medium, and can prevent external pollution, influences the accuracy of experimental result;
(2) the utility model divides the connecting rod into an upper section, a middle section and a lower section, which can suck liquid and sample, and the lower section of the connecting rod is also provided with metering scales, which can quantitatively take liquid;
(3) the utility model discloses still be equipped with the end ware, ware lid and end ware cooperation can arrange the culture medium in the end ware, cover the ware lid, then can carry out the process of coating fungus liquid in the environment sealed more, prevent that outer fungus from polluting.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic structural view of a coating brush according to the present invention;
fig. 3 is a schematic view of a connecting rod according to the present invention;
fig. 4 is a schematic structural view of the latch device according to the present invention;
fig. 5 is a schematic view of the combination of the vessel and the vessel cover according to the present invention.
Reference numerals:
1. a dish cover;
2. coating brush; 21. a rotating baffle;
3. a handle; 4. a drive motor;
5. a connecting rod;
51. a lower section; 52. a middle section; 53. an upper section;
54. a vertical rod; 55. a rubber ring; 56. measuring scales;
6. a coupling;
7. a clamping device;
71. fixing the rod; 72. a locking ring; 73. locking the bolt; 74. a threaded hole;
8. a switch; 9. and (7) a bottom dish.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1 to 5, the utility model provides an automatic coating ware for experiment is found to yeast library, including cylinder ware lid 1, this ware lid 1 inboard is fixed with coating brush 2, the culture medium that has the fungus liquid is arranged in ware lid 1, coating brush 2 coats the fungus liquid on the culture medium, it rotates to drive coating brush 2 through rotatory ware lid 1, it is that the fungus liquid evenly coats on the culture medium, this coating brush 2 sets up perpendicularly in the diameter direction of ware lid 1, this coating brush 2 can be square plate structure, it can be fixed in the interior top surface of ware lid 1, then the fungus liquid on the culture medium of accessible rotation carries out even coating, for coating more evenly, the bottom of coating brush 2 can be the cambered surface; because the culture medium is placed in the dish cover 1 for operation, the bottom end of the coating brush 2 is higher than the bottom end of the dish cover 1, so that the bottom end of the coating brush 2 can just contact with the upper surface of the culture medium to carry out bacteria liquid coating work on the culture medium; in addition, in order to conveniently observe the operation condition in the dish cover 1, the dish cover 1 can be made of transparent materials.
In order to enable the dish cover 1 and the coating brush 2 in the dish cover to rotate for coating operation, the top of the dish cover 1 is provided with a handle 3, a driving motor 4 can be arranged in the handle 3, and the dish cover 1 can rotate by being connected with the driving motor 4; the specific dish cover 1 can be provided with a connecting rod 5, the connecting rod 5 can be fixed with the dish cover 1, and the driving motor 4 can be rotationally connected with the connecting rod 5 through a coupler 6 so as to drive the dish cover 1 to rotate; in order to conveniently control the starting of the driving motor 4, a switch 8 is further arranged on the handle 3, and the switch 8 is electrically connected with the driving motor 4.
The connecting rod 5 can also be used for imbibition sampling, so that an additional bacteria liquid extractor is not needed to be used, and the operation is saved; the connecting rod 5 can movably penetrate through the dish cover 1 and the coating brush 2, and the connecting rod 5 is communicated with the inner side and the outer side of the dish cover 1 and can move up and down on the through hole; the connecting rod 5 can directly sample from the bottom surface of the dish cover 1 and then drop on the culture medium, so that the situation that the connecting rod 5 samples from the outside and can drip and splash bacteria liquid outside the dish cover 1 and the situation that the connecting rod 5 is polluted by the outside bacteria during external extraction is avoided, and the connecting rod 5 needs to be higher than the dish cover 1; because connecting rod 5 needs to extend to 1 bottom surface of ware lid and get the liquid dropping, then connecting rod 5 runs through the bottom of coating brush 2, connecting rod 5 can upwards move after accomplishing the sample dropping and keep away from the bottom surface of ware lid 1, at this moment the bottom of coating brush 2 runs through the position and need the closure to coat, for make coating brush 2 its bottom can be closed when connecting rod 5 upwards moves, can be equipped with gyration baffle 21 in the penetrating position that coating brush 2 bottom and connecting rod 5 correspond, the bottom of coating brush 2 is located to this gyration baffle 21 accessible slewing spring (not shown), when connecting rod 5 runs through coating brush 2 downwards, gyration baffle 21 opens downwards, after connecting rod 5 kept away from, the closed coating brush 2's of gyration baffle 21 automatic return bottom. The connecting rod 5 comprises a lower section 51, a middle section 52 and an upper section 53, wherein the lower section 51 can be a transparent hollow tube, the bottom end of the lower section 51 is a conical opening for facilitating liquid suction, the middle section 52 is a soft capsule suction tube, and the upper section 53 is a common cylinder, wherein the lower section 51 is communicated with the middle section 52 in a connecting way, and a bacterium liquid can be sucked and stored in the transparent hollow tube of the lower section 51 from the conical opening of the lower section 51 by pressing the soft capsule suction tube of the middle section 52 so as to be quantitatively taken out, and a metering scale 56 can be arranged on the outer wall of the transparent hollow tube of the lower section 51 for metering; the upper section 53 is fixedly connected to the lower section 51 via at least two vertical bars 54, and the middle section 52 is disposed in a space enclosed by the vertical bars 54, but the distance between the vertical bars 54 ensures that the vertical bars 54 can be pinched.
After the sampling liquid dropping is finished, the connecting rod 5 can be fixed with the dish cover 1 and then connected with the driving motor 4 in the handle 1 to drive the dish cover 1 to rotate, and the connecting rod can be fixed with the dish cover 1 through the clamping device 7; specifically, the clamping device 7 comprises a fixing rod 71, a locking ring 72 and a locking bolt 73 which are arranged at the upper end of the dish cover 1, the locking ring 72 can be fixed on the fixing rod 71, the connecting rod 5 can penetrate through the locking ring 72, a threaded hole 74 is formed in the locking ring 72, the locking bolt 73 is matched with the threaded hole 74, and the locking bolt 73 can penetrate through the threaded hole 74 and abut against the connecting rod 5 to lock the connecting rod 5 at a fixed position; in this embodiment, the locking bolt 73 abuts against the lower section 51 of the connecting rod 5, and since the lower section 51 is a transparent hollow tube, in order to prevent damage caused by abrasion during locking, a rubber ring 55 may be disposed at one end of the lower section 51 close to the middle section 52, and the locking bolt 73 abuts against and is locked on the rubber ring 55 for buffering.
As a preferred embodiment of the utility model, the utility model discloses the coating unit still is equipped with end dish 9, and ware lid 1 can mutually the buckle closure with end dish 9, can make the coating work go on in more sealed environment.
The utility model discloses a theory of operation is: during the use, can adorn the culture medium on end dish 9, connecting rod 5 absorbs the fungus liquid and drips into on the culture medium, then covers dish lid 1, is connected connecting rod 5 and the interior driving motor 4 of handle 3, starts driving motor 4, and connecting rod 5 drives dish lid 1 and rotates on end dish 9, and then makes coating brush 2 evenly coat the fungus liquid on the culture medium.
In the concrete implementation, the circular diameter of the dish cover 1 is about 140mm, and the height is about 20 mm; the height of the coating brush 2 is 20mm, and the width is 5 mm; the handle 3 has a diameter of 20mm and a height of 50 mm.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (9)
1. An automatic applicator for yeast library construction experiments, characterized by: the coating brush is fixed on the inner side of the dish cover and is vertically arranged in the diameter direction of the dish cover; a handle is arranged at the top of the dish cover, a driving motor is arranged in the handle, a connecting rod is arranged at the center of the dish cover, and the driving motor is rotationally connected with the connecting rod through a coupler; the connecting rod movably penetrates through the dish cover and the coating brush, and the connecting rod is higher than the dish cover; a rotary baffle is arranged at the bottom end of the coating brush and corresponds to the connecting rod, and the connecting rod is fixed with the dish cover through a clamping device;
the connecting rod includes hypomere, middle section and upper segment, the hypomere is transparent hollow tube and the bottom of this hypomere is the bell mouth, and the middle section is the flexible glue and inhales the bag pipe, and the upper segment is ordinary cylinder, the hypomere is connected with the middle section and is link up mutually, the upper segment is through at least two montants and hypomere fixed connection, the space that this montant encloses is arranged in to the middle section.
2. An automated applicator for yeast library construction experiments according to claim 1, wherein: the dish cover is made of transparent materials.
3. An automated applicator for yeast library construction experiments according to claim 1, wherein: the rotary baffle is arranged at the bottom end of the coating brush through a rotary spring.
4. An automated applicator for yeast library construction experiments according to claim 1, wherein: the coating brush is of a square plate structure, and the bottom end of the coating brush is an arc surface; the coating brush is fixed on the inner top surface of the dish cover, and the bottom end of the coating brush is higher than the bottom end of the dish cover.
5. An automated applicator for yeast library construction experiments according to claim 1, wherein: and a switch for controlling the driving motor is arranged on the handle.
6. An automated applicator for yeast library construction experiments according to claim 1, wherein: the clamping device comprises a fixed rod, a locking ring and a locking bolt, wherein the fixed rod, the locking ring and the locking bolt are arranged at the upper end of the dish cover, the locking ring is fixed on the fixed rod, the connecting rod penetrates through the locking ring, and the locking ring is provided with a threaded hole; the locking bolt is matched with the threaded hole to fix the connecting rod in position.
7. An automated applicator for yeast library construction experiments according to claim 6, wherein: and one end of the lower section of the connecting rod, which is close to the middle section, is provided with a rubber ring, and the locking bolt is propped against and locked on the rubber ring.
8. An automated applicator for yeast library construction experiments according to claim 1, wherein: and the outer wall of the lower section is also provided with metering scales.
9. An automated applicator for yeast library construction experiments according to claim 1, wherein: the automatic coating device is also provided with a bottom vessel, and the vessel cover and the bottom vessel are mutually buckled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022796010.4U CN213835318U (en) | 2020-11-27 | 2020-11-27 | Automatic coater for yeast library construction experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022796010.4U CN213835318U (en) | 2020-11-27 | 2020-11-27 | Automatic coater for yeast library construction experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213835318U true CN213835318U (en) | 2021-07-30 |
Family
ID=76993857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022796010.4U Expired - Fee Related CN213835318U (en) | 2020-11-27 | 2020-11-27 | Automatic coater for yeast library construction experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213835318U (en) |
-
2020
- 2020-11-27 CN CN202022796010.4U patent/CN213835318U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210730 |