CN108519371B - Automatic detector for food bacteria content - Google Patents
Automatic detector for food bacteria content Download PDFInfo
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- CN108519371B CN108519371B CN201810322384.7A CN201810322384A CN108519371B CN 108519371 B CN108519371 B CN 108519371B CN 201810322384 A CN201810322384 A CN 201810322384A CN 108519371 B CN108519371 B CN 108519371B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention relates to the field of food detection, in particular to an automatic food bacteria content detector, which comprises a liquid taking mechanism, a touch screen, a light emitting tube, an operation box, a feeding mechanism, a thermostat, a clamping mechanism, a control mechanism and a detection mechanism, wherein the liquid taking mechanism is arranged on the touch screen; the sample is extracted through the liquid taking mechanism, manual control is performed through the touch screen, the samples to be detected are packaged through the light emitting tubes, the operation box is used for controlling, the buffer solution and fluorescein mixed liquid is added through the feeding mechanism, the proper environment is improved through the constant temperature box, the light emitting tubes are clamped and fixed through the clamping mechanism, the feeding mechanism is controlled through the control mechanism, and detection is performed through the detection mechanism; the operation and detection positions are separated by the operation box and the thermostat, and meanwhile, the interference of other mixed bacteria can be effectively avoided by the independent closed space. The invention does not need to be held by an operator, can simultaneously carry out a plurality of detections, ensures the detection accuracy through comparison, reduces the operation labor force and improves the precision.
Description
Technical Field
The invention relates to the field of food detection, in particular to an automatic food bacteria content detector.
Background
The detection of the bacteria content of food is an important means for judging whether the food is safe or not, has an important function, most of the existing detection modes of the bacteria content adopt a microbial ATP bioluminescence method to determine the bacterial pollution degree in the food, the principle is that ATP of bacterial cells is made to emit light by utilizing biochemical action, the bacteria content is determined by the brightness of the ATP, and the detector is quick and sensitive in detection and wide in application range.
The existing detector is directly added with a buffer solution and a fluorescein mixture manually in an exposed environment before detection, mixed bacteria are easy to mix in the mode, and only one light-emitting tube can be detected at one time, so that great contingency exists. In view of this, the invention provides an automatic detector for food bacteria content, which has the following characteristics:
(1) according to the automatic food bacteria content detector, the operation box and the constant temperature box are utilized to separate the operation and detection positions, and meanwhile, the independent closed space can effectively avoid the interference of other mixed bacteria.
(2) According to the automatic food bacteria content detector, before detection, the three light emitting tubes are used for collecting samples, the clamping mechanism is used for clamping and fixing, the clamping mechanism does not need to be held by an operator, a plurality of detections can be carried out simultaneously, the detection accuracy is ensured through comparison, and the labor force during operation is reduced.
(3) According to the automatic food bacteria content detector, when the mixed solution of the buffer solution and the fluorescein is added, the mixed solution can be directly placed inside the packaging hole, and the buffer solution and the fluorescein can enter the inside of the plurality of light emitting tubes simultaneously in an extrusion mode, so that the labor force is greatly reduced, the error probability is reduced, and the detection precision is improved.
(4) According to the automatic food bacteria content detector, when the food enters the detection box body, the motor can be used for enabling the whole body to shake in a reciprocating mode, so that the interior is uniformly mixed, and meanwhile, the luminotron is covered before entering the detection box body, so that the luminotron can enter the detection box body for rapid detection.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an automatic food bacteria content detector, wherein a liquid taking mechanism is used for drawing a sample, a touch screen is used for manual control, a light-emitting tube is used for packaging the sample to be detected, an operation box is used for operation and control, a feeding mechanism is used for adding a buffer solution and a fluorescein mixed solution, a thermostat is used for improving a proper environment, a clamping mechanism is used for clamping and fixing the light-emitting tube, a control mechanism is used for controlling the feeding mechanism to perform detection, and a detection mechanism is used for detecting the sample; the operation and detection positions are separated by the operation box and the constant temperature box, and meanwhile, the interference of other mixed bacteria can be effectively avoided by an independent closed space; before detection, the three luminotrons are used for collecting samples, the clamping mechanism is used for clamping and fixing, the holding by an operator is not needed, a plurality of detections can be simultaneously carried out, the detection accuracy is ensured by contrast, and the labor force during operation is reduced; when the buffer solution and the fluorescein mixed solution are added, the buffer solution and the fluorescein mixed solution can be directly placed inside the container hole, and the buffer solution and the fluorescein mixed solution enter the inside of the plurality of luminous tubes simultaneously in an extrusion mode, so that the labor force is greatly reduced, the error probability is reduced, and the detection precision is improved; when entering into the inside that detects the box, usable motor makes holistic reciprocating rocking appear, ensures inside misce bene, carries out the closing cap to the luminotron before the entering simultaneously and is convenient for it to enter into and detects the box and carry out short-term test.
The technical scheme adopted by the invention for solving the technical problems is as follows: an automatic detector for food bacteria content comprises a liquid taking mechanism, a touch screen, a light emitting tube, an operation box, a feeding mechanism, a constant temperature box, a clamping mechanism, a control mechanism and a detection mechanism; the liquid taking mechanism for packaging and separating liquid is arranged in the operation box, and the liquid taking mechanism is matched and connected with the light emitting tube of the liquid separating packaging; the feeding mechanism for adding auxiliary materials is arranged in the operation box, the feeding mechanism is connected with the light emitting tube in a matching mode, and the control mechanism is arranged at the bottom of the feeding mechanism; the bottom of the operation box is fixed with the constant temperature box, and the clamping mechanism for clamping and moving the light emitting tube is arranged in the constant temperature box; the inside of thermostated container is equipped with detection mechanism, get liquid mechanism reinforced mechanism the thermostated container fixture the control mechanism with the equal electric connection of detection mechanism the touch-sensitive screen.
Specifically, the liquid taking mechanism comprises a liquid collecting bottle, a fixing ring, an electromagnetic valve and a first blanking head, wherein the fixing ring is fixed on the side wall of the operation box and is connected with the liquid collecting bottle in a clamping manner; the bottom of the liquid collecting bottle is communicated with the electromagnetic valve, and the electromagnetic valve is electrically connected with the touch screen; the bottom of the electromagnetic valve is connected with the first blanking head through a hose; the number of the first blanking heads is three, and the number and the distance of the first blanking heads are the same as those of the light emitting tubes.
Specifically, two symmetrical circular hole-shaped operating holes are formed in the surface of the operating box, and the bottom of the operating box is welded with the constant temperature box; a transparent window is arranged on the side part of the constant temperature box and is rotatably connected with the constant temperature box; and a handle is fixed on the side part of the transparent window.
Specifically, the feeding mechanism comprises a pull rod, a connecting rod, a second blanking head, a cover plate, a connecting plate, a piston, a containing hole and a containing box; the container box is fixed at the top of the operation box, and two rows of container holes are formed in the container box; the bottoms of the container holes are communicated with the second blanking heads, and the second blanking heads penetrate through the container box; the piston is connected to the inside of the packaging hole in a sliding mode, and the connecting rod is fixed to the top of the piston; the top of the connecting rod is fixed with the connecting plate, and the top of the connecting plate is fixed with the pull rod; the pull rod is connected with the cover plate in a sliding mode, and the cover plate is connected with the container box in a clamping mode.
Specifically, the number and the interval of the single-row packaging holes are the same as those of the first lower stub bars, and the height of the packaging holes and the height of the connecting rods are the same as those of the cover plates with the bottoms of the openings and the hollow structures.
Specifically, the clamping mechanism comprises a motor, a first electric push rod, a connecting rod and a clamping paw, the motor is embedded into the thermostat, and one end of the motor is connected with the first electric push rod; one end of the first electric push rod is fixed with the connecting rod, and the side part of the connecting rod is fixed with three clamping claws.
Specifically, the number and the interval of the clamping claws are the same as those of the first blanking head, and the clamping claws comprise clamping plates, limiting plates, second electric push rods and plate teeth; the limiting plate is fixed on the side part of the connecting rod, and the second electric push rod is fixed inside the limiting plate; one side of the second electric push rod is connected with the clamping plate, and the clamping plate is rotatably connected with the limiting plate; the inner side of the clamping plate is provided with the plate teeth; the splint adopt semi-circular structure, just the inboard equidistance of splint is equipped with a plurality of rubber construction the plate tooth.
Specifically, the control mechanism comprises a press switch, a movable plate and a first torsion spring, the movable plate is connected to the inside of the incubator through the first torsion spring, and the press switch is arranged on the side of the movable plate; the press switch is electrically connected with the first electric push rod and the touch screen.
Specifically, the detection mechanism comprises a light barrier, a detection box body, a feed port, a second torsion spring and a baffle plate, the detection box body is fixed inside the incubator, and the feed port is formed in the side part of the detection box body; the top of feed inlet is fixed the baffle, just the lateral part of feed inlet passes through second torsional spring connects the barn door.
Specifically, the baffle is fixed at the top of the feed inlet at an inclination angle of 30-45 degrees, and a plurality of protrusions are arranged at the bottom of the baffle; the side part of the baffle is provided with a groove structure; the sectional area of the feed inlet is the same as that of the light emitting tubes, and the distance and the number of the feed inlets are the same as those of the light emitting tubes.
The invention has the beneficial effects that:
(1) according to the automatic food bacteria content detector, the operation box and the constant temperature box are utilized to separate the operation and detection positions, and meanwhile, the independent closed space can effectively avoid the interference of other mixed bacteria.
(2) According to the automatic food bacteria content detector, before detection, the three light emitting tubes are used for collecting samples, the clamping mechanism is used for clamping and fixing, the clamping mechanism does not need to be held by an operator, a plurality of detections can be carried out simultaneously, the detection accuracy is ensured through comparison, and the labor force during operation is reduced.
(3) According to the automatic food bacteria content detector, when the mixed solution of the buffer solution and the fluorescein is added, the mixed solution can be directly placed inside the packaging hole, and the buffer solution and the fluorescein can enter the inside of the plurality of light emitting tubes simultaneously in an extrusion mode, so that the labor force is greatly reduced, the error probability is reduced, and the detection precision is improved.
(4) According to the automatic food bacteria content detector, when the food enters the detection box body, the motor can be used for enabling the whole body to shake in a reciprocating mode, so that the interior is uniformly mixed, and meanwhile, the luminotron is covered before entering the detection box body, so that the luminotron can enter the detection box body for rapid detection.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of an automatic food bacteria content detector according to a preferred embodiment of the present invention;
FIG. 2 is a schematic structural view of the charging mechanism shown in FIG. 1;
FIG. 3 is a schematic view of the clamping mechanism shown in FIG. 2;
FIG. 4 is a schematic view of the gripper jaw shown in FIG. 3;
FIG. 5 is a schematic view of the control mechanism shown in FIG. 1;
FIG. 6 is a schematic structural view of the detecting mechanism shown in FIG. 1;
fig. 7 is a side view of the baffle shown in fig. 6.
In the figure: 1. the liquid taking mechanism comprises a liquid taking mechanism 11, a liquid collecting bottle 12, a fixing ring 13, an electromagnetic valve 14, a first blanking head 2, a touch screen 3, a light emitting tube 4, an operation box 41, an operation hole 5, a feeding mechanism 51, a pull rod 52, a connecting rod 53, a second blanking head 54, a cover plate 55, a connecting plate 56, a piston 57, a container hole 58, a container box 6, a thermostat 61, a transparent window 62, a handle 7, a clamping mechanism 71, a motor 72, a first electric push rod 73, a connecting rod 74, a clamping claw 741, a clamping plate 742, a limiting plate 743, a second electric push rod 744, a plate tooth 8, a control mechanism 81, a press switch 82, a movable plate 83, a first torsion spring 9, a detection mechanism 91, a light blocking plate 92, a detection box body 93, a feeding hole 94, a second torsion spring 95, a baffle 951 and a protrusion.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1, the automatic food bacteria content detector of the present invention comprises a liquid taking mechanism 1, a touch screen 2, a light emitting tube 3, an operation box 4, a feeding mechanism 5, a thermostat 6, a clamping mechanism 7, a control mechanism 8 and a detection mechanism 9; the liquid taking mechanism 1 for packaging and separating liquid is arranged in the operation box 4, and the liquid taking mechanism 1 is matched and connected with the light emitting tube 3 of the liquid separating packaging; the feeding mechanism 5 for adding auxiliary materials is arranged in the operation box 4, the feeding mechanism 5 is connected with the light-emitting tube 3 in a matching manner, and the control mechanism 8 is arranged at the bottom of the feeding mechanism 5; the bottom of the operation box 4 is fixed with the constant temperature box 6, and the clamping mechanism 7 for clamping and moving the light-emitting tube 3 is arranged in the constant temperature box 6; the inside of thermostated container 6 is equipped with detection mechanism 9, get liquid mechanism 1 the reinforced mechanism 5 the thermostated container 6 fixture 7 the control mechanism 8 with the equal electric connection of detection mechanism 9 touch-sensitive screen 2.
Specifically, as shown in fig. 1, the liquid taking mechanism 1 includes a liquid collecting bottle 11, a fixing ring 12, an electromagnetic valve 13 and a first blanking head 14, the fixing ring 12 is fixed on the side wall of the operation box 4, and the fixing ring 12 is connected with the liquid collecting bottle 11 in a clamping manner; the bottom of the liquid collecting bottle 11 is communicated with the electromagnetic valve 13, and the electromagnetic valve 13 is electrically connected with the touch screen 2; the bottom of the electromagnetic valve 13 is connected with the first blanking head 14 through a hose; first stub bar 14 is equipped with threely, just quantity, the interval of first stub bar 14 with luminotron 3 interval, quantity are the same, and the sample that will accomplish the preliminary treatment is arranged in the inside of collecting liquid bottle 11, when carrying out the detection of bacterial content, opens solenoid valve 13 makes the sample that needs the detection pass through first stub bar 14 enters into the inside of luminotron 3 realizes the branch liquid to the sample, and a plurality of detections of being convenient for go on simultaneously, can realize the contrast of experiment.
Specifically, as shown in fig. 1, two symmetrical circular hole-shaped operation holes 41 are formed in the surface of the operation box 4, and the thermostat 6 is welded to the bottom of the operation box 4; a transparent window 61 is arranged on the side of the incubator 6, and the transparent window 611 is rotatably connected with the incubator 6; a handle 62 is fixed to a side portion of the transparent window 61, the inside of the operation box 4 can be manually operated through the operation hole 41, and the inside of the incubator 6 can be operated and treated by opening the transparent window 61.
Specifically, as shown in fig. 1 and fig. 2, the charging mechanism 5 includes a pull rod 51, a connecting rod 52, a second blanking head 53, a cover plate 54, a connecting plate 55, a piston 56, a containing hole 57 and a containing box 58; the container box 58 is fixed on the top of the operation box 4, and two rows of container holes 57 are formed in the container box 58; the bottoms of the container holes 57 are communicated with the second blanking heads 53, and the second blanking heads 53 penetrate through the container boxes 58; the piston 56 is connected in a sliding manner inside the containing hole 57, and the connecting rod 52 is fixed at the top of the piston 56; the top of the connecting rod 52 is fixed with the connecting plate 55, and the top of the connecting plate 55 is fixed with the pull rod 51; the pull rod 51 is slidably connected to the cover plate 54, the cover plate 54 is engaged with the container 58, the cover plate 54 is opened, the buffer solution and the fluorescein mixture to be added are placed in the container hole 57, the cover plate 54 is closed, the pull rod 51 can be pressed during addition, the piston 56 is lowered to press the container hole 57, and the raw material to be added falls under pressure from the second blanking head 53.
Specifically, as shown in fig. 1 and 2, the number and the pitch of the container holes 57 in a single row are the same as those of the first lower stub bars 14, and the height of the container holes 57 and the height of the connecting rod 52 are the same as those of the cover plate 54 having an open and hollow bottom, so that a buffer solution and a mixed solution of fluorescein are added to the inside of each light-emitting tube 3.
Specifically, as shown in fig. 1 and 3, the clamping mechanism 7 includes a motor 71, a first electric push rod 72, a connecting rod 73 and a clamping claw 74, the motor 71 is embedded inside the oven 6, and one end of the motor 71 is connected to the first electric push rod 72; the connecting rod 73 is fixed to one end of the first electric push rod 72, the three clamping claws 74 are fixed to the side portion of the connecting rod 73, the light emitting tube 3 can be driven to move to one side by opening the first electric push rod 72, and the light emitting tube 3 can be shaken by opening the motor 71 to rotate in a reciprocating manner.
Specifically, as shown in fig. 3 and 4, the number and the intervals of the clamping claws 74 are the same as those of the first blanking head 14, and the clamping claws 74 include clamping plates 741, limiting plates 742, second electric push rods 743 and plate teeth 744; the limit plate 742 is fixed to a side of the link 73, and the second electric putter 743 is fixed to an inside of the limit plate 742; one side of the second electric push rod 743 is connected to the clamping plate 741, and the clamping plate 741 is rotatably connected to the limiting plate 742; the inner side of the clamping plate 741 is provided with the plate tooth 744; the clamping plate 741 is of a semicircular structure, the plate teeth 744 of a plurality of rubber structures are equidistantly arranged on the inner side of the clamping plate 741, the clamping plate 741 can be opened when the second motor 743 is opened to extend the second motor, the light-emitting tube 3 is put down, and the clamping plate 741 can be closed to clamp the light-emitting tube 3 when the second electric push rod 743 retracts.
Specifically, as shown in fig. 1 and 5, the control mechanism 8 is disposed at the bottom end of the charging mechanism 5, the control mechanism 8 includes a push switch 81, a movable plate 82 and a first torsion spring 83, the movable plate 82 is connected to the inside of the oven 6 through the first torsion spring 83, and the push switch 81 is disposed on the side of the movable plate 82; the pressing switch 81 is electrically connected to the first electric push rod 72 and the touch screen 2, and when the clamping mechanism 7 moves to press the movable plate 82, the first electric push rod 72 is closed to stop moving, so that the material feeding is realized.
Specifically, as shown in fig. 1 and 6, the detection mechanism 9 includes a light barrier 91, a detection box 92, a feeding hole 93, a second torsion spring 94 and a baffle 95, the detection box 92 is fixed inside the oven 6, and the feeding hole 93 is opened at a side portion of the detection box 92; the top of feed inlet 93 is fixed baffle 95, just the lateral part of feed inlet 93 passes through second torsional spring 94 connects barn door 91 fixture 7 will when luminotron 3 pushed into, luminotron 3's lid because baffle 95's the stopping can make the lid rotatory, along with luminotron 3's entering, because feed inlet 93's restriction makes the lid close, opens the barn door 91 sends into detect the inside of box 92 and detect.
Specifically, as shown in fig. 6 and 7, the baffle 95 is fixed at the top of the feed port 93 by adopting an inclination angle of 30 ° to 45 °, and the bottom of the baffle 95 is provided with a plurality of protrusions 951; the side part of the baffle 95 is provided with a groove structure; the sectional area of the feed port 93 is the same as that of the light emitting tube 3, and the pitch and number of the feed ports 93 are the same as those of the light emitting tube 3, so that the cover of the light emitting tube 3 can be closed conveniently.
The liquid taking mechanism 1 is used for drawing a sample, the touch screen 2 is used for manual control, the luminescent tube 3 is used for packaging the sample to be detected, the operation box 4 is used for operation and control, the feeding mechanism 5 is used for adding a buffer solution and a fluorescein mixed solution, the constant temperature box 6 is used for providing a proper environment, the clamping mechanism 7 is used for clamping and fixing the luminescent tube 3, the feeding mechanism 5 is controlled by the control mechanism 8, and the detection mechanism 9 is used for detection; the method specifically comprises the following steps:
(1) firstly, the pretreated and cultured sample is placed in the liquid collecting bottle 11, when bacteria content is detected, the clamping plate 741 can be closed to clamp the light-emitting tube 3 when the second electric push rod 743 retracts, and the electromagnetic valve 13 is opened after clamping is finished, so that the sample to be detected enters the light-emitting tube 3 through the first discharge head 14, liquid separation of the sample is realized, and a plurality of detections are simultaneously performed.
(2) Then, the first electric push rod 72 is opened to drive the light emitting tube 3 to move to one side, when the clamping mechanism 7 moves to press the movable plate 82, the first electric push rod 72 is closed to stop the movement, at this time, the cover plate 54 is opened to place the buffer solution and the mixed solution of the fluorescein to be added into the container hole 57, the cover plate 54 is closed, when the buffer solution and the mixed solution of the fluorescein are added, the pull rod 51 is pressed to lower the piston 56 to press the container hole 57, and the raw material to be added falls from the second blanking head 53 under pressure.
(3) After the feeding is finished, the motor 71 is turned on to rotate in a reciprocating manner, so that the light-emitting tube 3 can shake, and the inside is fully mixed; the first electric push rod 72 is continuously pushed to move rightwards, when the first electric push rod reaches the side part of the detection mechanism 9, the clamping mechanism 7 pushes the light-emitting tube 3 in, the cover of the light-emitting tube 3 can rotate due to the blocking of the baffle 95, the cover is closed due to the limitation of the feed port 93 along with the entering of the light-emitting tube 3, the light barrier 91 is opened and is sent into the detection box body 92 for detection, and the bacterial pollution degree in the food is measured by a microbial ATP bioluminescence method.
According to the automatic detector for the bacteria content of the food, the operation and detection positions are separated through the operation box 4 and the constant temperature box 6, and meanwhile, the independent closed space can effectively avoid the interference of other mixed bacteria; before detection, three light-emitting tubes 3 are used for packaging samples, the clamping mechanism 7 is used for clamping and fixing, the holding by an operator is not needed, a plurality of detections can be simultaneously carried out, the detection accuracy is ensured through comparison, and the labor force during operation is reduced; when the buffer solution and the fluorescein mixed solution are added, the buffer solution and the fluorescein mixed solution can be directly placed in the container hole 57, and the buffer solution and the fluorescein mixed solution enter the plurality of light-emitting tubes 3 simultaneously in an extrusion mode, so that the labor force is greatly reduced, the error probability is reduced, and the detection precision is improved; when entering the interior of the detection box body 92, the motor 71 can be utilized to make the whole body generate reciprocating shaking, so as to ensure uniform internal mixing, and meanwhile, the luminotron 3 is sealed before entering so as to be convenient for entering the detection box body 92 to carry out rapid detection.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. The utility model provides a food contains fungus volume automated inspection appearance which characterized in that: comprises a liquid taking mechanism (1), a touch screen (2), a light-emitting tube (3), an operation box (4), a feeding mechanism (5), a constant temperature box (6), a clamping mechanism (7), a control mechanism (8) and a detection mechanism (9); the liquid taking mechanism (1) for packaging and separating liquid is arranged in the operation box (4), and the liquid taking mechanism (1) is matched and connected with the light emitting tube (3) of the liquid separating packaging; the feeding mechanism (5) for adding auxiliary materials is arranged in the operation box (4), the feeding mechanism (5) is connected with the light-emitting tube (3) in a matching mode, and the control mechanism (8) is arranged at the bottom of the feeding mechanism (5); the bottom of the operation box (4) is fixed with the constant temperature box (6), and the clamping mechanism (7) for clamping and moving the light-emitting tube (3) is arranged in the constant temperature box (6); the detection mechanism (9) is arranged in the thermostat (6), and the liquid taking mechanism (1), the feeding mechanism (5), the thermostat (6), the clamping mechanism (7), the control mechanism (8) and the detection mechanism (9) are all electrically connected with the touch screen (2);
the liquid taking mechanism (1) comprises a liquid collecting bottle (11), a fixing ring (12), an electromagnetic valve (13) and a first blanking head (14), the fixing ring (12) is fixed on the side wall of the operation box (4), and the fixing ring (12) is connected with the liquid collecting bottle (11) in a clamping mode; the bottom of the liquid collecting bottle (11) is communicated with the electromagnetic valve (13), and the electromagnetic valve (13) is electrically connected with the touch screen (2); the bottom of the electromagnetic valve (13) is connected with the first lower stub bar (14) through a hose; the number of the first blanking heads (14) is three, and the number and the interval of the first blanking heads (14) are the same as those of the light-emitting tubes (3);
two symmetrical circular hole-shaped operating holes (41) are formed in the surface of the operating box (4), and the thermostat (6) is welded at the bottom of the operating box (4); a transparent window (61) is arranged on the side part of the constant temperature box (6), and the transparent window (61) is rotatably connected with the constant temperature box (6); a handle (62) is fixed on the side part of the transparent window (61);
the feeding mechanism (5) comprises a pull rod (51), a connecting rod (52), a second blanking head (53), a cover plate (54), a connecting plate (55), a piston (56), a containing hole (57) and a containing box (58); the container box (58) is fixed at the top of the operation box (4), and two rows of container holes (57) are formed in the container box (58); the bottoms of the container holes (57) are communicated with the second blanking heads (53), and the second blanking heads (53) penetrate through the container boxes (58); the inner part of the containing hole (57) is connected with the piston (56) in a sliding way, and the top of the piston (56) is fixed with the connecting rod (52); the top of the connecting rod (52) is fixed with the connecting plate (55), and the top of the connecting plate (55) is fixed with the pull rod (51); the pull rod (51) is connected with the cover plate (54) in a sliding mode, and the cover plate (54) is connected with the container box (58) in a clamping mode;
the number and the interval of the single-row collecting holes (57) are the same as those of the first lower stub bars (14), and the height of the collecting holes (57) and the height of the connecting rods (52) are the same as those of the cover plates (54) with the bottoms of the openings and the hollow structures;
the clamping mechanism (7) comprises a motor (71), a first electric push rod (72), a connecting rod (73) and a clamping paw (74), the motor (71) is embedded into the incubator (6), and one end of the motor (71) is connected with the first electric push rod (72); one end of the first electric push rod (72) is fixed with the connecting rod (73), and three clamping claws (74) are fixed on the side part of the connecting rod (73);
the number and the distance between the clamping claws (74) are the same as those of the first lower stub bars (14), and the clamping claws (74) comprise clamping plates (741), limiting plates (742), second electric push rods (743) and plate teeth (744); the limit plate (742) is fixed on the side of the connecting rod (73), and the second electric push rod (743) is fixed inside the limit plate (742); one side of the second electric push rod (743) is connected with the clamping plate (741), and the clamping plate (741) is rotatably connected with the limiting plate (742); the inner side of the clamping plate (741) is provided with the plate teeth (744); the clamping plate (741) is of a semicircular structure, and a plurality of plate teeth (744) with rubber structures are arranged on the inner side of the clamping plate (741) at equal intervals;
the control mechanism (8) comprises a press switch (81), a movable plate (82) and a first torsion spring (83), the movable plate (82) is connected to the inside of the incubator (6) through the first torsion spring (83), and the press switch (81) is arranged on the side of the movable plate (82); the press switch (81) is electrically connected with the first electric push rod (72) and the touch screen (2);
the detection mechanism (9) comprises a light barrier (91), a detection box body (92), a feeding hole (93), a second torsion spring (94) and a baffle (95), the detection box body (92) is fixed inside the constant temperature box (6), and the feeding hole (93) is formed in the side part of the detection box body (92); the top of the feed port (93) is fixed with the baffle (95), and the side part of the feed port (93) is connected with the light barrier (91) through the second torsion spring (94);
the baffle (95) is fixed at the top of the feed port (93) by adopting an inclination angle of 30-45 degrees, and a plurality of protrusions (951) are arranged at the bottom of the baffle (95); the side part of the baffle (95) is provided with a groove structure; the sectional area of the feed opening (93) is the same as that of the light-emitting tube (3), and the distance and the number of the feed openings (93) are the same as those of the light-emitting tube (3);
the liquid taking mechanism (1) is used for drawing samples, the touch screen (2) is used for manual control, the luminescent tube (3) is used for packaging the samples to be detected, the operation box (4) is used for operation and control, the feeding mechanism (5) is used for adding a buffer solution and a fluorescein mixed solution, the thermostat (6) is used for providing a proper environment, the luminescent tube (3) is clamped and fixed by the clamping mechanism (7), the feeding mechanism (5) is controlled by the control mechanism (8), and the detection is carried out by the detection mechanism (9); the method specifically comprises the following steps:
firstly, placing a pretreated and cultured sample in the liquid collecting bottle (11), enabling the clamping plate (741) to be closed to clamp the light-emitting tube (3) when the second electric push rod (743) retracts during detection of bacteria content, and opening the electromagnetic valve (13) after clamping is finished, so that the sample to be detected enters the light-emitting tube (3) through the first blanking head (14), so that liquid separation of the sample is realized, and a plurality of detections are facilitated to be carried out simultaneously;
then, the first electric push rod (72) is opened to drive the light-emitting tube (3) to move to one side, when the clamping mechanism (7) moves to press the movable plate (82), the first electric push rod (72) can be closed to stop moving, at the moment, the cover plate (54) is opened to place the buffer solution and the fluorescein mixed solution which need to be added respectively in the container hole (57), the cover plate (54) is covered, when adding, the pull rod (51) can be pressed, the piston (56) is made to descend to press the container hole (57), so that the raw material which needs to be added falls from the second blanking head (53) under pressure;
after the feeding is finished, the motor (71) is turned on to rotate in a reciprocating manner, so that the light-emitting tube (3) can shake, and the inside is fully mixed; the first electric push rod (72) is enabled to continuously push the light-emitting tube (3) to move rightwards, when the light-emitting tube reaches the side part of the detection mechanism (9), the clamping mechanism (7) pushes the light-emitting tube (3) into the cover, the cover of the light-emitting tube (3) can be rotated due to the blocking of the baffle (95), the cover is closed due to the limitation of the feed inlet (93) along with the entering of the light-emitting tube (3), the light barrier (91) is opened and is sent into the detection box body (92) for detection, and the bacterial contamination degree in food is measured by a microbial ATP bioluminescence method.
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| CN108519371B true CN108519371B (en) | 2020-11-20 |
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| CN112067763B (en) * | 2020-09-14 | 2021-04-13 | 广东嘉士利食品集团有限公司 | Food production contains bacterial content detector |
| CN114828474B (en) * | 2022-03-31 | 2024-02-27 | 国家电网有限公司 | Multifunctional protective screen eyebrow device |
| CN116004375B (en) * | 2023-02-21 | 2023-06-13 | 威海德生技术检测有限公司 | Device and method for detecting microorganism content in ocean food |
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| CN101113985A (en) * | 2006-07-27 | 2008-01-30 | 中国科学院电子学研究所 | Sanitary status on-site rapid detection device and detecting method |
| CN105132274A (en) * | 2015-09-22 | 2015-12-09 | 陕西延长石油(集团)有限责任公司研究院 | Full-automatic bacteria disappearing experimental instrument |
| CN105974072A (en) * | 2016-05-03 | 2016-09-28 | 成都亿信标准认证集团有限公司 | Food safety detection system |
| CN206292245U (en) * | 2016-11-04 | 2017-06-30 | 珠海迪尔医疗设备研究院有限公司 | A kind of reagent pipetting volume rifle |
| CN206863009U (en) * | 2017-06-14 | 2018-01-09 | 广东灿城农产品集团有限公司 | A kind of quick continuous detection apparatus of food security |
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| US7496225B2 (en) * | 2003-09-04 | 2009-02-24 | 3M Innovative Properties Company | Biological growth plate scanner with automated intake |
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| CN101113985A (en) * | 2006-07-27 | 2008-01-30 | 中国科学院电子学研究所 | Sanitary status on-site rapid detection device and detecting method |
| CN105132274A (en) * | 2015-09-22 | 2015-12-09 | 陕西延长石油(集团)有限责任公司研究院 | Full-automatic bacteria disappearing experimental instrument |
| CN105974072A (en) * | 2016-05-03 | 2016-09-28 | 成都亿信标准认证集团有限公司 | Food safety detection system |
| CN206292245U (en) * | 2016-11-04 | 2017-06-30 | 珠海迪尔医疗设备研究院有限公司 | A kind of reagent pipetting volume rifle |
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