CN213819292U - Culture dish frame, rotating frame and biological sample form imaging device - Google Patents

Abstract

The embodiment of the disclosure discloses a culture dish rack, a rotating rack and biological sample form imaging equipment. The culture dish frame comprises a base, a rotary plate and a servo motor. The turntable is fixed on the base, and a fixing device is arranged on the turntable; the servo motor is fixed on the base and connected with the turntable. According to the embodiment of the disclosure, the turntable can be controlled to rotate through the servo motor, and various environments with gravity changes can be simulated.

Description

Culture dish frame, rotating frame and biological sample form imaging device

Technical Field

The utility model relates to a biological instrument technical field, concretely relates to culture dish frame, swivel mount and biological sample form imaging device.

Background

Currently, some of the heavy studies on plant seedlings rely on weightless environments, for example, Paul et al published papers in 2012, which investigated the root tip growth direction of arabidopsis seeds after germination under weightless conditions by conducting experiments in the environment of international space stations. However, this type of study is cost prohibitive and difficult to reproduce by the general research team.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the related art, embodiments of the present disclosure provide a culture dish rack, a rotating rack, and a biological sample form imaging apparatus.

In a first aspect, an embodiment of the present disclosure provides a culture dish rack.

Specifically, the culture dish frame includes:

a base;

the rotary table is fixed on the base and is provided with a fixing device; and

and the servo motor is fixed on the base and connected with the turntable.

With reference to the first aspect, in a first implementation manner of the first aspect, two fixing holes are respectively disposed on two opposite sides of the base.

With reference to the first aspect, the present disclosure provides in a second implementation form of the first aspect, the fixing device includes a screw or a spring clip.

With reference to the first aspect, in a third implementation manner of the first aspect, the fixing device includes at least three sets of spring blocking pieces, the spring blocking pieces can move in a plane where the turntable is located, and the spring blocking pieces provide pressure to the center direction of the turntable under the action of a spring.

In combination with the first aspect, in a fourth implementation manner of the first aspect, the culture dish rack further comprises at least two fixing claws, the fixing claws are oppositely arranged at the top of the base, clamping grooves are formed in the upper portions of the fixing claws, and the culture light source is detachably mounted on the fixing claws through the clamping grooves.

With reference to the fourth implementation manner of the first aspect, in a fifth implementation manner of the first aspect, the culture light source includes a light source and a light uniformizing plate, and the light uniformizing plate is disposed on a light propagation path of the light source.

With reference to the fourth implementation manner of the first aspect, in a sixth implementation manner of the first aspect, the culture light source includes an LED aluminum substrate, multiple paths of lamp beads are arranged on the LED aluminum substrate, and at least two of white light sources, red light sources, infrared light sources, blue light sources, ultraviolet light sources, and green light sources are combined and packaged in the lamp beads.

In a second aspect, a swivel is provided in embodiments of the present disclosure.

Specifically, the rotating frame includes:

rotating the platform;

the bracket body is fixed on the rotating platform and comprises a first bracket and a second bracket, the second bracket is arranged in a radial shape, the first bracket is fixed on the second bracket, and an electric socket interface is arranged on the first bracket;

the culture dish holder according to the first aspect as well as any one of the first to sixth implementation manners of the first aspect is detachably attached between the two adjacent first holders, and a connection wire of the servo motor is connected to the electrical outlet interface.

In a third aspect, the disclosed embodiments provide a biological specimen morphology imaging apparatus.

Specifically, the biological sample morphology imaging apparatus includes:

the incubator is provided with an observation window;

a swivel mount according to the second aspect, provided inside the incubator; and

and the imaging module is used for acquiring the image of the biological sample in the culture dish rack on the rotating frame through the observation window.

According to the technical scheme that this disclosure embodiment provides, through the base, fix carousel and servo motor on the base, wherein, be provided with fixing device on the carousel, servo motor with the carousel links to each other to accessible fixing device installs the culture dish on the carousel, drives the carousel through servo motor and rotates, and then control culture dish rotates, can realize the change of gravity direction, can be used to simulate weightless environment even.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

Drawings

Other features, objects, and advantages of the present disclosure will become more apparent from the following detailed description of non-limiting embodiments when taken in conjunction with the accompanying drawings. In the drawings:

fig. 1A and 1B show schematic views of a gravity culture dish rack according to an embodiment of the present disclosure;

FIG. 2 shows a schematic view of a rotating gantry according to an embodiment of the present disclosure;

fig. 3 shows a schematic diagram of a biological specimen morphology imaging apparatus according to an embodiment of the present disclosure;

FIG. 4 shows a flow chart of a control method according to an embodiment of the disclosure;

FIG. 5A shows a schematic diagram of the distribution of the plant root tip orientation under normal cultivation conditions;

fig. 5B shows a schematic of a distribution of plant root tip directions under gravity perturbed conditions according to an embodiment of the present disclosure.

Detailed Description

Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. Also, for the sake of clarity, parts not relevant to the description of the exemplary embodiments are omitted in the drawings.

In the present disclosure, it is to be understood that terms such as "including" or "having," etc., are intended to indicate the presence of the disclosed features, numbers, steps, behaviors, components, parts, or combinations thereof, and are not intended to preclude the possibility that one or more other features, numbers, steps, behaviors, components, parts, or combinations thereof may be present or added.

It should be further noted that the embodiments and features of the embodiments in the present disclosure may be combined with each other without conflict. The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Currently, some of the heavy studies on young plants depend on a weightless environment, for example, Paul et al published a paper in 2012, which studies the direction of root tip growth after germination of arabidopsis seeds under weightless conditions by conducting experiments in international space stations. However, this type of study is cost prohibitive and difficult to reproduce by the general research team.

In order to solve the problems in the related art, the inventor designs a culture dish rack which comprises a base, a rotary plate and a servo motor, wherein the rotary plate and the servo motor are fixed on the base, the rotary plate is provided with a fixing device, the servo motor is connected with the rotary plate, so that a culture dish can be installed on the rotary plate through the fixing device, the rotary plate is driven to rotate through the servo motor, the culture dish is further controlled to rotate, the change of the gravity direction can be realized, and the culture dish rack can be even used for simulating the weightless environment.

Fig. 1A and 1B show schematic views of a culture dish rack according to an embodiment of the present disclosure.

As shown in fig. 1A and 1B, the culture dish holder includes:

a base 67;

the turntable 68 is fixed on the base 67, and a fixing device 72 is arranged on the turntable 68; and

and a servo motor 69 fixed on the base 67 and connected with the turntable 68.

According to the embodiment of the present disclosure, the center of the turntable 68 is connected to a servo motor 69, the servo motor 69 is fixed on the base 67, and the fixing device 72 can be used for fixing the culture dish 71. The control circuit sends a signal to the servo motor 69 to control the turntable 68 to drive the culture dish 71 to rotate by a fixed angle so as to change the gravity direction, or to rotate at a constant speed at a certain rotation speed so as to simulate the weightlessness status.

According to the embodiment of the present disclosure, two fixing holes 73 are respectively disposed on two opposite sides of the base. The spacing of the fixing holes is designed to match with a rotating frame described below, and the fixing holes are used for mounting the culture dish rack on the rotating frame and occupying one rack position of the rotating frame.

The fixing device 72 may be, for example, a screw or a spring clip for fixing the culture dish 71 according to the embodiment of the present disclosure. The fixing device 72 can also be at least three sets of spring stopping sheets, the spring stopping sheets can move in the plane of the turntable, and the spring stopping sheets provide pressure to the center direction of the turntable under the action of springs. According to the embodiment of the disclosure, the culture dish 71 can be placed on the turntable 68 by pulling the spring blocking piece open, the included angle between the spring blocking piece and the turntable can be smaller than 90 degrees, and the spring blocking piece provides pressure inwards to fix the culture dish 71, so that the culture dish is prevented from displacement in the using process.

According to the embodiment of the disclosure, the culture dish holder may further include at least two fixing claws 70, the fixing claws 70 are oppositely disposed on the top of the base, a clamping groove is disposed on the upper portion of the fixing claw 70, and the culture light source 44 is detachably mounted on the fixing claw 70 through the clamping groove. The method is used for combined treatment of gravity conditions and illumination conditions, and provides more choices for experimental design of scientific research.

According to the embodiment of the present disclosure, the fixing claw 70 may be fixed on the top of the base 67, and a clamping groove is formed on one side of the fixing claw 70, and the clamping groove may be matched with protrusions at two ends of the cultivation light source 44 to fix the cultivation light source 44 on the fixing claw 70. The base 67 may include a back plate and three side plates, the three side plates are respectively disposed at two sides and a bottom of the back plate, the fixing holes 73 may be disposed on the side plates at two sides, and the side plates are not disposed at a top thereof, so that light of the culture light source may irradiate the culture dish 71. The culture light source 44 can be quickly disassembled and assembled through the fixing claw 70 so as to meet different requirements of different periods or different test items on illumination in the plant culture process.

According to the embodiment of the present disclosure, the cultivation light source 44 includes a light source and a light homogenizing plate disposed on a light propagation path of the light source. In order to reduce the difference of the lighting effect of different positions of the culture light source, the light homogenizing plate is arranged on the culture light source, so that light can be uniformly irradiated on a sample, and the experimental error caused by nonuniform light is reduced.

According to the embodiment of the present disclosure, the cultivation light source 44 includes an LED aluminum substrate, multiple paths of beads are arranged on the LED aluminum substrate, and at least two of white light, red light, infrared light, blue light, ultraviolet light, and green light sources are combined and packaged in the beads.

According to the embodiment of the present disclosure, the cultivation light source may select an LED light source for cultivation as needed. The LED aluminum substrate 43 of the LED light source for cultivation is simultaneously provided with a plurality of paths of lamp beads, can respectively provide light sources with various wavelengths such as far-red light, green light, blue light, white light, ultraviolet light and the like, and can respectively and independently control the on-off and the light intensity of each path of light by the control circuit of the device. Various monochromatic lights, especially wavelengths which can be simultaneously started such as red light, far-red light, blue light and the like are combined and packaged in one lamp bead, so that the situation that the position and the illumination direction of a light source are different when different wavelengths are started, and the consistency of experimental conditions is influenced is avoided. For white light and each monochromatic light which cannot be simultaneously started, the lamp beads can be divided into two groups for packaging and are alternately arranged so as to meet the requirement of larger output power. One LED light source for culture can simultaneously realize the control of at least 4 light sources with different colors. Different lamp pearls can be set up to different cultivation light source 44, can select suitable cultivation light source 44 to install on stationary dog 70 as required.

Fig. 2 shows a schematic view of a rotating gantry according to an embodiment of the present disclosure.

As shown in fig. 2, the rotating frame includes:

a rotary platform 14;

the bracket body is fixed on the rotating platform 14 and comprises a first bracket 111 and a second bracket 112, the second bracket 112 is radially arranged, the first bracket 111 is fixed on the second bracket 112, and the first bracket 111 is provided with an electrical socket interface 13; and

the culture dish rack as described in fig. 1A and 1B is detachably mounted between the two adjacent first brackets 111, and the connection of the servo motor 69 is connected to the electrical outlet interface 13. The wiring of the culture light source 44 may also be connected to the electrical outlet interface 13 according to embodiments of the present disclosure.

According to the embodiment of the present disclosure, at least one rack mount 12 is fixed to each first bracket 111. The culture dish rack can be fixed between the rack fixing pieces 12 of two adjacent first brackets 111. For example, at least two mounting holes are respectively formed on both sides of the rack-position fixing member 12, and the rack-position fixing member is engaged with the fixing holes 73 of the culture dish rack and fixed by a screw or other parts. The carousel may be used to hold a variety of culture dish racks, and may be used for other types of culture dish racks in addition to the culture dish rack 39 depicted in FIGS. 1A and 1B, such as standard small vertical culture dish racks 36, large horizontal culture dish racks 37, small airtight culture dish racks 38, and the like. Among them, the standard small vertical culture dish rack 36, the small airtight culture dish rack 38, and the culture dish rack 39 as described in fig. 1A and 1B occupy one rack position on the rotating rack, and the large horizontal culture dish rack 37 occupies two rack positions on the culture dish rack.

The utility model provides a swivel mount through general frame position mounting, can fix a plurality of culture dish framves on a plurality of adjacent installation positions, under rotary platform's drive, imaging device (for example CCD camera or CMOS camera) can be aimed at respectively to different culture dish framves, and then can conveniently carry out the imaging analysis to the biological sample in a plurality of culture dish framves simultaneously, has improved sample analysis flux, improves analysis efficiency.

Fig. 3 shows a schematic diagram of a biological specimen morphology imaging apparatus according to an embodiment of the present disclosure.

As shown in fig. 3, the biological sample morphology imaging apparatus includes:

an incubator 4 on which an observation window is provided;

a rotating frame 5 as described in fig. 2, disposed inside the incubator 4; and

and the imaging module 3 is used for acquiring the image of the biological sample in the culture dish rack on the rotating rack 5 through the observation window.

According to the embodiment of the disclosure, the biological sample morphological imaging device may further include a housing 1 and an optical isolation platform 2, and the rotating frame 5 may pass through an opening at the bottom of the incubator 4 and be fixed on the optical isolation platform 2.

According to an embodiment of the present disclosure, the biological sample may be a plant sample, in particular a plant seedling sample. The biological sample form imaging device provided by the embodiment of the disclosure can be a young plant imaging device, and is used for analyzing the growth state of a young plant. A culture dish holder as illustrated in fig. 1A and 1B may be mounted on the rotating frame 5 to fix a culture dish. The servo motor capable of controlling the culture dish rack drives the culture dish to rotate or stop rotating in the vertical plane. The incubator 4 can flexibly adjust the growth environment of the plant seedlings, including temperature, gas component concentration and the like, and meets the control requirements of various experimental condition variables. The observation window of the side wall of the incubator 4 can enable the imaging module 3 outside the incubator to acquire image data of the young plants inside the incubator 4 for studying the growth change of the young plants.

According to embodiments of the present disclosure, the culture dish rack, the rotating rack, and the biological specimen morphology imaging apparatus described above may be used to change the direction in which the plant experiences gravity. By controlling the servo motor to rotate a given angle, the gravity direction sensed by the plant sample on the culture dish can be quantitatively changed. By utilizing the real-time dynamic imaging of the device, the response change of the plant seedlings to gravity can be observed and analyzed.

The disclosed embodiment provides a control method for controlling the culture dish rack described above, including:

acquiring angle information;

and controlling the servo motor to drive the turntable to rotate to a target position based on the angle information.

The experimental procedure is described below by way of example for studying the root-weighted response of Arabidopsis seedlings:

a) surface sterilization of arabidopsis seeds: placing a proper amount of seeds in a 1.5mL centrifuge tube, soaking in 75% alcohol and 0.01% Triton X-100, shaking for 10min, and pouring out the liquid; adding 95% alcohol to wash once, and pouring out the liquid; covering a pipe cover tightly after the pipe cover is completely air-dried in an open way in a superclean workbench;

b) preparing a culture medium: 4.33g/L of Murashige-Skoog salt, 10g/L of cane sugar and 8g/L of Phytagel plant gel are added with deionized water to prepare 1L of culture solution, and KOH and HCl are used for adjusting the pH value to 5.7-5.8; sterilizing at 121 ℃ for 15min, cooling to about 60 ℃, pouring into a sterile transparent circular plastic culture dish with the diameter of about 90mm in a super clean bench, wherein the thickness of the culture medium is about 3-4 mm; standing, cooling and solidifying for later use; the culture medium of the embodiment of the disclosure adopts Phytagel as a coagulant, and is more transparent compared with agar, so that higher imaging definition can be obtained conveniently;

c) sowing and seed germination: sowing the sterilized seeds on the surface of a culture medium by using tweezers in a super-clean workbench, and arranging the seeds in a row at intervals of about 5 mm; closing the culture dish cover, and sealing by using a sealing film; placing in a dark environment at 4 ℃ for imbibition for 4 days, taking out, vertically placing, culturing for 5 days at 22 ℃ under light, and vertically growing the arabidopsis thaliana seedlings by clinging to the surface of a culture medium;

d) mounting the culture dish frame: screwing 4M 6 × 12 hexagon socket screws into fixing holes on two sides of the base 67 of the culture dish rack, sliding into mounting holes on the rotating rack, screwing and fixing, and inserting the wiring of the servo motor 69 and the culture light source 44 into the electric socket interface 13;

e) sample loading: the culture dish sealing film is detached, the back surface faces outwards, the cover faces inwards, and the culture dish sealing film is fixed on the rotary table 68, so that the interference of dew condensation of the front cover can be avoided; a layer of black flocking cloth or hard paper coated with black light absorption coating is clamped between the culture dish and the rotating disc so as to improve the contrast between the plant sample and the background during shooting;

f) starting the modules of the biological sample morphology imaging device: the closed incubator 4 is controlled by computer software to provide a constant temperature environment of 22 ℃ for the incubator; continuously providing fresh air into the incubator; controlling the overhead light source 44 to provide hybrid white light illumination; turning on a front infrared imaging illumination module arranged on the imaging module, and providing illumination from the side front of the sample by using 940nm infrared light; controlling the imaging module and the rotating frame to perform continuous dynamic imaging on the culture dish on each culture dish frame, namely moving the camera position of the imaging module at set time intervals (such as 5min) and rotating the rotating frame to perform one round of shooting on each culture dish;

g) changing the gravity direction: at the moment of experiment presetting, controlling servo motors on the culture dish racks through computer software, respectively reading respective current positions, calculating position values after clockwise rotation by 90 degrees, and respectively moving to corresponding positions; if the shooting of all samples in one round is not finished at the preset moment of the experiment, the servo motor is operated after the shooting of the samples in the round is finished;

h) continuously carrying out continuous dynamic imaging until the preset experimental time; and analyzing the acquired image data, and researching the influence of the change of the gravity direction on the growth direction of the root of the arabidopsis thaliana.

In addition, the culture dish rack, the rotating rack and the biological sample form imaging device provided by the embodiment of the disclosure can enable the direction of gravity relative to the plant to be changed continuously through continuous rotation, and disturb the influence of the gravity on the plant so as to simulate a weightless environment. By utilizing the real-time dynamic imaging of the device, the response change of the plant seedlings to gravity can be observed and analyzed.

Fig. 4 shows a flow chart of a control method according to an embodiment of the present disclosure. As shown in fig. 4, the control method includes the following steps S410 to S420:

in step S410, the servo motor is controlled to drive the turntable to rotate continuously;

in step S420, in response to obtaining the first control instruction, a target angle is determined from at least two candidate angles, and the dial is controlled to stop at the target angle.

According to this disclosed embodiment, servo motor drives carousel and culture dish and lasts rotatoryly in vertical plane, can disturb the influence of gravity to the plant sample in the culture dish to simulate weightless environment.

According to the embodiment of the disclosure, the first control instruction may be generated based on a photographing instruction, for example, and is used for controlling the servo motor to stop rotating, so that the turntable and the culture dish stop rotating, and the photographing definition is improved. The dial may be controlled to continue rotating after a predetermined time, or in response to obtaining a second control command.

According to the embodiment of the present disclosure, at least two candidate angles, for example, 0 degree and 180 degrees, may be set in advance, and more candidate angles, for example, 0 degree, 120 degrees, and 240 degrees, may also be set. In response to obtaining the first control instruction, the control dial is stopped at one of the candidate angles, i.e., the target angle. Because more than two candidate angles exist, the camera does not stop at the same position every time of photographing, and the gravity accumulation effect in the photographing process is weakened.

For example, a first angle among the candidate angles, for example, 0 degree, is stopped when the number of times the dial is stopped is an odd number; and stops at a second angle different from the first angle, for example, 180 degrees, among the candidate angles when the number of times the turntable stops is an even number.

It will be understood by those skilled in the art that the second angle may be other than 180 degrees, and three, four or more preset candidate angles or random angles may be used to reduce the effect of gravity accumulation during the photographing process and better simulate the weightless environment, which is not limited by the present disclosure.

According to the embodiment of the disclosure, images are acquired after the servo motor is paused, and because the angle of the turntable is different during pausing, the direction of the culture dish in the images obtained by exposure is also different. The image alignment can be carried out by adopting an image feature recognition-affine transformation method, and all images are corrected to be in a uniform orientation.

One of ordinary skill in the art will appreciate that other methods of image registration and correction to a uniform orientation may be used, and the present disclosure is not limited thereto.

According to the technical scheme provided by the embodiment of the disclosure, the servo motor is controlled to drive the turntable to rotate continuously, a target angle is determined from at least two candidate angles in response to the acquisition of a first control instruction, and the turntable is controlled to stop at the target angle, so that the shooting definition can be improved, and the cumulative effect of gravity is weakened at the same time. On the other hand, shooting at a given candidate angle is stopped, so that later image alignment and analysis are facilitated.

The following will explain the mechanism of the influence of gravity on morphogenesis of Arabidopsis seeds in germination as an example.

a) Seed surface sterilization: placing an appropriate amount of Arabidopsis seeds in a 1.5mL centrifuge tube, soaking in 75% alcohol and 0.01% Triton X-100, shaking for 10min, and pouring out the liquid; adding 95% alcohol to wash once, and pouring out the liquid; covering a pipe cover tightly after the pipe cover is completely air-dried in an open way in a superclean workbench;

b) preparing a culture medium: 4.33g/L of Murashige-Skoog salt, 10g/L of cane sugar and 8g/L of Phytagel plant gel are added with deionized water to prepare 1L of culture solution, and KOH and HCl are used for adjusting the pH value to 5.7-5.8; sterilizing at 121 ℃ for 15min, cooling to about 60 ℃, pouring into a sterile transparent circular plastic culture dish with the diameter of about 90mm in a super clean bench, wherein the thickness of the culture medium is about 3-4 mm; standing, cooling and solidifying for later use;

c) sowing: using tweezers to sow the sterilized seeds on the surface of the culture medium in a clean bench, and uniformly spreading the seeds on the whole culture medium at intervals of about 5 mm; closing the culture dish cover, and sealing by using a sealing film; placing the glass plate in a dark environment at 4 ℃ for imbibition for 4 days, taking out the glass plate and preparing for mounting;

d) mounting the culture dish frame: the culture dish rack is arranged on the rotating frame 5 through fixing holes on two sides of the culture dish rack base 67, and the wiring of the servo motor 69 and the overhead culture light source 44 is inserted into the electric socket interface 13;

e) sample loading: the culture dish sealing film is removed to weaken the dewing on the cover and promote the exchange of internal and external gases, the back surface faces outwards, the cover faces inwards and is fixed on the rotary table 68, a layer of black flocking cloth or hard paper coated with black light absorption coating can be clamped between the culture dish and the rotary table, so that the contrast between the plant sample and the background during shooting is improved;

f) starting up the various device modules of the biological sample imaging system: the incubator 4 is controlled by computer software to provide a constant temperature environment of 22 ℃ for the incubator, and fresh air is continuously provided for the incubator; controlling the overhead light source 44 to provide hybrid white light illumination; turning on a front infrared imaging illumination module, and providing illumination from the side front of the sample by using 940nm infrared light; controlling an imaging module to perform continuous dynamic imaging on the culture dish on each culture dish rack, namely moving the position of a camera and rotating a main bracket at set time intervals (such as 5min) and performing one-round shooting on each culture dish;

g) simulating a weightlessness environment: from the beginning of an experiment, the servo motor 69 is controlled to continuously rotate clockwise through computer software, the rotating speed is controlled to be 5-15 r/min, the influence of radial acceleration on plant growth cannot be weakened too fast, so that systematic errors are avoided, and the effect of gravity interference cannot be weakened too slowly; when shooting is needed, a camera and a rotating frame of an imaging module are moved to a position corresponding to a target; controlling the rotating position of a servo motor corresponding to the culture dish to be shot, stopping when the rotation of the servo motor is rotated to 0 degree in the odd-numbered shooting wheels, and stopping when the rotation of the servo motor is rotated to 180 degrees in the even-numbered shooting wheels, and continuing to rotate other servo motors; the camera immediately exposes and shoots, and the rotation of the corresponding servo motor is immediately restarted after the exposure is finished, wherein the direction and the rotating speed are the same as the previous direction;

h) continuously carrying out continuous dynamic imaging until the preset experimental time; and statistically analyzing the growth orientation distribution of the root tip and the hypocotyl of the germinated seeds.

Fig. 5A shows a schematic distribution of plant root tip directions under normal cultivation conditions, and fig. 5B shows a schematic distribution of plant root tip directions under gravity disturbance conditions according to an embodiment of the present disclosure, in which the length of a bar in the figure represents a ratio of the number of roots in each direction, and an arrow in fig. 5A points to the direction of gravity.

The method of the embodiment of the disclosure can effectively weaken the accumulation of the gravity effect in a certain direction. Under normal gravity conditions, as shown in fig. 5A, the direction of the root tip of the arabidopsis seedling is centered in the vertical direction; under the condition of simulating weightlessness by the method in the embodiment of the disclosure, as shown in fig. 5B, the root tip directions are uniformly distributed in all directions, the experimental result is consistent with the growth state of the plant seedlings in the international space station reported by Paul et al in 2012, and the effectiveness of the method in simulating weightlessness environment is verified. Therefore, the technical scheme of the embodiment of the disclosure can simulate the weightless environment in space in the ground environment, and provides a feasible method on the ground for the phytology research which must be carried out on a space station in the past.

The foregoing description is only exemplary of the preferred embodiments of the disclosure and is illustrative of the principles of the technology employed. It will be appreciated by those skilled in the art that the scope of protection covered by this disclosure is not limited to the particular combination of features described above, but also encompasses other arrangements formed by any combination of features described above or their equivalents without departing from the scope of the present disclosure. For example, the above features and (but not limited to) the features disclosed in this disclosure having similar functions are replaced with each other to form the technical solution.

Claims (8)

1. A culture dish holder, comprising:

a base;

the rotary table is fixed on the base and is provided with a fixing device; and

and the servo motor is fixed on the base and connected with the turntable.

2. The culture dish holder according to claim 1, wherein two fixing holes are provided on each of two opposite sides of the base.

3. The culture dish holder of claim 1, wherein the securing means comprises a screw or a spring clip.

4. The culture dish holder according to claim 1, wherein the fixing device comprises at least three groups of spring blocking pieces, the spring blocking pieces can move in a plane where the turntable is located, and the spring blocking pieces provide pressure to the center direction of the turntable under the action of the springs.

5. The culture dish holder according to claim 1, further comprising at least two fixing claws, wherein the fixing claws are oppositely arranged at the top of the base, clamping grooves are formed in the upper portions of the fixing claws, and the culture light source is detachably mounted on the fixing claws through the clamping grooves.

6. The culture dish holder according to claim 5, wherein:

the culture light source comprises a light source and a light homogenizing plate, and the light homogenizing plate is arranged on a light propagation path of the light source; and/or

The cultivation light source comprises an LED aluminum substrate, a plurality of paths of lamp beads are arranged on the LED aluminum substrate, and at least two of white light sources, red light sources, infrared light sources, blue light sources, ultraviolet light sources and green light sources are combined and packaged in the lamp beads.

7. A swivel stand, comprising:

rotating the platform;

the bracket body is fixed on the rotating platform and comprises a first bracket and a second bracket, the second bracket is arranged in a radial shape, the first bracket is fixed on the second bracket, and an electric socket interface is arranged on the first bracket;

the culture dish holder according to any one of claims 1 to 6, detachably mounted between two adjacent first holders, wherein a connection of the servo motor is connected to the electrical outlet interface.

8. A biological specimen morphology imaging apparatus, comprising:

the incubator is provided with an observation window;

the swivel stand of claim 7, disposed inside the incubator; and

and the imaging module is used for acquiring the image of the biological sample in the culture dish rack on the rotating frame through the observation window.

Images