CN213924782U

CN213924782U - Culture dish frame fixed bolster, incubator and biological sample form imaging device

Info

Publication number: CN213924782U
Application number: CN202022690890.7U
Authority: CN
Inventors: 纪玉锶
Original assignee: Beijing Mccollen Technology Co ltd
Current assignee: Beijing Mccollen Technology Co ltd
Priority date: 2020-11-19
Filing date: 2020-11-19
Publication date: 2021-08-10
Anticipated expiration: 2030-11-19

Abstract

The embodiment of the present disclosure discloses culture dish frame fixed bolster, incubator and biological sample form imaging device, culture dish frame fixed bolster includes: the bracket comprises a bracket body and a bracket fixing piece; the bracket body comprises a plurality of first brackets arranged at intervals, and at least one bracket position fixing piece is fixed on each first bracket; the rack position fixing piece comprises two rows of mounting positions, and the adjacent mounting positions on the rack position fixing piece of the adjacent two first supports are used for fixing a culture dish rack. This technical scheme can be fixed a plurality of culture dish framves on a plurality of adjacent installation positions through general frame position mounting, then utilizes image device to carry out the image analysis to the biological sample in the culture dish frame in proper order, has improved sample analysis flux.

Description

Culture dish frame fixed bolster, incubator and biological sample form imaging device

Technical Field

The utility model relates to a biological sample detects technical field, concretely relates to culture dish frame fixed bolster, incubator and biological sample form imaging equipment.

Background

In conventional biological research, only the static state of an organism at a certain time section can be researched by experimental means, and the cumulative effect of all biological dynamic processes before the time point is reflected. However, the growth and development of organisms are a combination of a series of time-space specific processes, and the space-time specificity of the regulation and control of the growth and development of organisms can be more completely understood only by observing dynamic change processes.

The prior art provides a device which needs to be placed in a dark constant-temperature greenhouse to maintain the stability of the growth environment of organisms, and controls the movement of a camera to switch the field of view of the camera among samples so as to observe the growth and development of the organisms under different space-time characteristics. However, this device cannot simultaneously analyze large-scale samples, and only provides a single experimental environment, and cannot satisfy the most basic conditions for applying a normal culture light source and controlling the growth of organisms such as temperature, oxygen, carbon dioxide, and the like.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the related art, the embodiment of the present disclosure provides a culture dish rack fixing support, an incubator, and a biological sample form imaging apparatus.

In a first aspect, an embodiment of the present disclosure provides a culture dish rack fixing bracket.

Specifically, culture dish frame fixed bolster includes: the bracket comprises a bracket body and a bracket fixing piece; the bracket body comprises a plurality of first brackets arranged at intervals, and at least one bracket position fixing piece is fixed on each first bracket; the rack position fixing piece comprises two rows of mounting positions, and the adjacent mounting positions on the rack position fixing piece of the adjacent two first supports are used for fixing a culture dish rack.

Optionally, the rack mount fixture includes: the bracket comprises a bracket fixing part, a first frame position part and a second frame position part which are arranged in pair; the first frame position part and the second frame position part are respectively positioned at two sides of the bracket fixing part; the first frame position part and the second frame position part are provided with at least two mounting holes; the mounting holes form a row of mounting positions.

Optionally, the first and second housing portions are respectively disposed at obtuse angles to the bracket fixing portion.

Optionally, the mounting hole is a through hole structure or a U-shaped groove.

Optionally, the rack position fixing pieces are fixed at different height positions of the same first rack; and/or

The rack position fixing pieces are fixed at the positions with the same height of the plurality of first supports.

Optionally, the heights of the mounting holes on two rack position fixing pieces used for fixing the same culture dish rack are matched, and the heights of the two rack position fixing pieces are the same or different.

Optionally, one row of mounting positions of the rack position fixing piece consists of two mounting holes, and is used for mounting a small culture dish rack on one side of the rack position fixing piece; or

The rack position fixing piece is provided with a row of mounting positions, each row of mounting positions comprises more than four mounting holes and is used for mounting more than two small culture dish racks or at least one large culture dish rack on the left side or the right side of the rack position fixing piece; or

The length of two adjacent first support's frame position mounting is different, longer frame position mounting with at least one shorter on the frame position mounting the high phase-match of mounting hole.

Optionally, an electrical socket interface is further arranged on the first support and used for electrically connecting with the culture dish rack.

Optionally, the electrical socket interface comprises an electrical line connector having control pins.

Optionally, the control pin in the electrical line connector comprises:

light source common cathode/common anode pin and/or

Overhead 1 st light source pin and/or

Overhead 2 nd-way light source pin and/or

Overhead 3 rd light source pin and/or

Overhead 4 th light source pin and/or

Side light source pin and/or

Servo motor power supply pin and/or

A servo motor ground pin and/or

Servo motor data bus pin.

Optionally, the first bracket is further provided with an air pump fixing plate, an air pump damping sleeve and an air pump; the air pump fixing plate is fixedly provided with a plurality of air pump damping sleeves, and the air pump is arranged inside the air pump damping sleeves; the air pump is used for exchanging air inside and outside the culture dish frame.

Optionally, a pneumatic connector is further arranged on the first support, connected with a gas pipeline of the gas pump, and used for conveying gas into the culture dish rack.

Optionally, a liquid conveying joint is further arranged on the first support and used for conveying liquid into the culture dish rack.

Optionally, the culture dish rack fixing bracket further comprises:

the bracket body is fixedly connected with the rotating platform;

the support body further comprises second supports which are distributed in a radial mode, and the first supports are fixed at the end portions, extending out of the rotating platform, of the second supports.

Optionally, the rotating platform comprises: the device comprises a base, a flange fixed on the base, a baffle and a photoelectric limit switch fixed on the base;

the separation blade is fixed at the gap of the flange, and the height of the separation blade is matched with that of the photoelectric limit switch; the blocking piece is provided with a notch, and the optical signal of the photoelectric limit switch can pass through the notch.

Optionally, still be provided with electric socket interface on the first support, culture dish frame fixed bolster still includes:

the distributing plate is fixed above the rotating platform;

and a conductive slip ring is arranged in the rotating platform, and a cable connected with the electric socket interface is converged by the distributing board and then is electrically connected with the conductive slip ring.

Optionally, a mounting hole is formed in the top of the rotating platform, and the second bracket and the distributing board are fixed through the mounting hole; the second bracket is fixed between the distributing board and the rotating platform;

the rotating platform is further provided with a central through hole, and cables connected with the electric socket interface are converged by the distributing board and then electrically connected with the conductive slip ring through the central through hole.

Optionally, the culture dish rack fixing bracket further comprises: an electrical equipment cover fixed above the second bracket; and an air pump pipe outlet is formed in the electrical equipment cover.

Optionally, a liquid delivery joint is further disposed on the first support and connected to a liquid pipeline of the conductive slip ring.

Optionally, the fluid delivery fitting is disposed laterally of the electrical receptacle interface.

Optionally, the mounting hole comprises: the support mounting holes and the distributing plate mounting holes; the support mounting holes and the distributing plate mounting holes are arranged at intervals.

In a second aspect, embodiments of the present disclosure provide an incubator.

Specifically, the culture dish rack fixing support as any one of the first aspect is arranged in the incubator.

In a third aspect, embodiments of the present disclosure provide a biological specimen morphology imaging apparatus.

Specifically, the biological sample morphology imaging apparatus includes: an incubator and an imaging apparatus according to the second aspect; the imaging device is used for shooting the form of the biological sample in the incubator.

The technical scheme provided by the embodiment of the disclosure can have the following beneficial effects:

the culture dish frame fixed bolster that this disclosed embodiment provided through general frame position mounting, can fix a plurality of culture dish framves on a plurality of adjacent installation positions, then utilizes imaging device (for example CCD camera or CMOS camera) to carry out the imaging analysis to the culture dish in proper order frame biological sample, has improved sample analysis flux.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

Drawings

Other features, objects, and advantages of the present disclosure will become more apparent from the following detailed description of non-limiting embodiments when taken in conjunction with the accompanying drawings. In the drawings:

figure 1 shows a front view of a culture dish rack-securing support according to an embodiment of the present disclosure;

FIG. 2 illustrates a schematic structural view of a rack mount according to an embodiment of the present disclosure;

FIG. 3 illustrates a schematic structural view of an electrical connection between a conductive slip ring to an electrical receptacle interface according to an embodiment of the present disclosure;

figure 4 shows a schematic view of a culture dish rack secured by a culture dish rack securing rack according to an embodiment of the present disclosure;

FIG. 5 shows the schematic view of FIG. 1 with the electrical equipment enclosure removed;

figure 6 shows a top view of a culture dish rack securing bracket according to an embodiment of the present disclosure;

FIG. 7 is a schematic diagram illustrating the position relationship of an electro-optic limit switch according to an embodiment of the present disclosure;

FIG. 8 shows a schematic structural view of an incubator according to an embodiment of the present disclosure;

fig. 9 shows a schematic structural diagram of a biological sample morphological imaging device according to an embodiment of the present disclosure.

Detailed Description

Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. Also, for the sake of clarity, parts not relevant to the description of the exemplary embodiments are omitted in the drawings.

In the present disclosure, it is to be understood that terms such as "including" or "having," etc., are intended to indicate the presence of the disclosed features, numbers, steps, behaviors, components, parts, or combinations thereof, and are not intended to preclude the possibility that one or more other features, numbers, steps, behaviors, components, parts, or combinations thereof may be present or added.

It should be further noted that the embodiments and features of the embodiments in the present disclosure may be combined with each other without conflict. The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

The prior art provides a device which needs to be placed in a dark greenhouse with constant temperature to maintain the stability of the plant growth environment, and controls the movement of a camera to switch the camera view among samples so as to observe the growth and development of organisms under different space-time characteristics. However, this device cannot simultaneously analyze large-scale samples, and only provides a single experimental environment, and cannot satisfy the most basic conditions for applying a normal culture light source and controlling the growth of organisms such as temperature, oxygen, carbon dioxide, and the like.

In basic scientific research of botany, including but not limited to environmental stress resistance (high temperature resistance, freezing resistance, salt resistance, oxygen deficiency resistance and oxidation damage resistance), optical signal path, photosynthesis, plant hormone signal path, plant developmental biology, plant breeding and other directions of plants, dynamic space-time specificity needs to be researched, and therefore an experimental technology matched with the dynamic space-time specificity needs to be researched.

The present disclosure is made to solve, at least in part, the problems in the prior art that the inventors have discovered.

As shown in fig. 1 to 7, an embodiment of the present disclosure provides a culture dish rack fixing bracket, where the culture dish rack fixing bracket 1 includes: a bracket body 11 and a bracket fixing piece 12; the bracket body 11 comprises a plurality of first brackets 111 arranged at intervals, and at least one bracket fixing piece 12 is fixed on each first bracket 111; the rack position fixing part 12 comprises two rows of mounting positions, and adjacent mounting positions on the rack position fixing part 12 of two adjacent first brackets 111 are used for fixing a culture dish rack.

According to an embodiment of the present disclosure, the shelf mount 12 includes: a holder fixing portion 121, a first seating portion 122 and a second seating portion 123 provided in a pair; the first and

second housing parts

122 and 123 are respectively located at both sides of the bracket fixing part 121; wherein the first shelf portion 122 and the second shelf portion 123 are each provided with at least two mounting holes; the mounting holes form a row of mounting positions.

In an embodiment of the present disclosure, the first shelf portion 122 and the second shelf portion 123 are respectively disposed at an obtuse angle to the holder fixing portion 121, so as to assume an open and closed state, thereby facilitating the operation of fixing the culture dish holder.

In a manner of an embodiment of the present disclosure, the mounting holes include an upper mounting hole a1 and a lower mounting hole a 2; the upper mounting hole A1 and the lower mounting hole A2 form a column of mounting positions.

In a mode of the embodiment of the present disclosure, the upper mounting hole a1 and the lower mounting hole a2 may be through holes, or may also be U-shaped grooves or L-shaped grooves, and a screw, a nut, or other parts may be used to pass through the through holes or the U-shaped grooves or push into a transverse portion of the L-shaped grooves and then slide down into a vertical portion to fix the culture dish support, which is not limited by the present disclosure.

In the embodiment of the present disclosure, a row of mounting positions is not limited to two mounting holes, and a row of mounting positions may also be formed by a plurality of mounting holes, and an appropriate mounting hole on the mounting position is selected according to the size of the culture dish fixing frame for fixing, which is not limited by the present disclosure.

According to the embodiment of the present disclosure, the rack mount fixing pieces 12 are fixed at different height positions of the same first bracket 111; and/or the rack position fixing pieces 12 are fixed at the same height position of a plurality of the first brackets 111.

In the mode of the embodiment of the present disclosure, a plurality of rack position fixing pieces 12 may be disposed on each first support 111, and the rack position fixing pieces 12 disposed on different first supports 111 have the same height, so that the mounting positions on different first supports 111 are close to each other, and the mounting positions are conveniently used to fix a plurality of culture dish racks on the support main body 11 in a layered manner, thereby increasing the number of the fixed culture dish racks.

It can be understood that if a greater number of culture dish racks need to be fixed, the height of the first bracket 111 and the number of the rack position fixing pieces 12 need to be increased correspondingly, and more layers of installation positions are set, which is not described in detail in this disclosure.

According to the embodiment of the disclosure, the heights of the mounting holes on the two rack-position fixing pieces 12 for fixing the same culture dish rack are matched, and the heights of the two rack-position fixing pieces 12 are the same or different.

In the mode of the embodiment of the present disclosure, the two rack position fixing pieces 12 for fixing the same culture dish rack may be a universal piece, and the mounting holes on the mounting positions of the two rack position fixing pieces having the same height are aligned, so that the culture dish rack is convenient to fix.

In an embodiment of the present disclosure, the rack position fixing members 12 on the plurality of first brackets 111 may also be arranged at positions staggered by one or more mounting holes, and positions of other mounting holes are ensured to be aligned, and it is also possible to fix a plurality of culture dish brackets by using mounting positions in a layered manner, which is not limited by the present disclosure.

According to the embodiment of the disclosure, one row of mounting positions of the rack position fixing piece 12 consists of two mounting holes, and is used for mounting a small culture dish rack on one side of the rack position fixing piece 12; or

A row of mounting positions of the rack position fixing piece 12 consists of more than four mounting holes and is used for mounting more than two small culture dish racks or at least one large culture dish rack on the left side or the right side of the rack position fixing piece 12; or

The lengths of the frame fixing pieces 12 of two adjacent first brackets 111 are different, and the height of the mounting hole on the longer frame fixing piece 12 is matched with the height of the mounting hole on at least one shorter frame fixing piece 12.

In the mode of the embodiment of the present disclosure, for example, two mounting holes may be formed in the rack mount 12, or four mounting holes may be formed as a row of mounting positions. The fixed small-sized culture dish rack generally needs two mounting holes to ensure stability, so that the rack position fixing part 12 of the two mounting holes can be used for fixing one small-sized culture dish rack, or the rack position fixing part 12 of the four mounting holes can be used for fixing two small-sized culture dish racks. Of course, a large culture dish rack can be fixed by the rack position fixing piece 12 with four mounting holes, so that stability is guaranteed. By analogy, culture dish racks with different sizes and numbers can be installed according to the number of the installation holes formed in the rack fixing part 12, which is not limited by the disclosure.

In the mode of the embodiment of the present disclosure, the rack position fixing parts 12 may be non-universal parts, that is, the lengths of the rack position fixing parts 12 of two adjacent first brackets 111 may be different, when fixing the culture dish rack, the longer rack position fixing part 12 matches the height of the mounting hole on at least one shorter rack position fixing part 12, for example, after the mounting holes are aligned or dislocated from the position of one or more mounting holes, the other mounting holes are aligned, and the culture dish rack may be fixed.

According to the embodiment of the disclosure, an electrical socket interface 13 is further arranged on the first support 111, and is used for electrically connecting with the culture dish rack. The electrical outlet interfaces 13 may be fixed to each first bracket 111, and the number may be flexibly set according to the requirement, which is not limited by the present disclosure.

According to an embodiment of the present disclosure, the electrical outlet interface 13 comprises: an electrical wiring connector having control pins.

In an aspect of an embodiment of the present disclosure, an electrical line connector may include: the 9-pin flat cable socket can simultaneously control 5 paths of light sources and a plurality of paths of servo motors respectively.

The specific stitch definition is as follows:

1-common cathode/common anode of light source, 2-overhead 1 st light source, 3-overhead 2 nd light source, 4-overhead 3 rd light source, 5-overhead 4 th light source, 6-side light source, 7-servo motor power supply (VCC pin), 8-servo motor ground (GND pin) and 9-servo motor DATA bus (DATA pin).

Light sources with different wavelengths and/or light intensities can be arranged in the culture dish frame and used as culture light sources of the biological samples. The electrical socket interface 13 is used to electrically connect with a light source in the culture dish rack to provide light source conditions for culturing biological samples. For example, an overhead culture light source and a side culture light source may be provided on the dish holder. The overhead culture light source is located at the top of the culture dish frame, the side culture light source is located on the side of the culture dish frame, the 9-pin plug is used for the overhead culture light source, but the cable is only connected with 1-5 pins, and the cable is only connected with 1 pin and 6 pins for the side culture light source. The gravity module can also be fixed to the culture dish frame, and the gravity direction of biological sample in the culture dish can be changed to the gravity module, and to the gravity module, the cable only connects 7 ~ 9 feet. All modules adopt 9-pin plugs with the same specification, and can be inserted into any one electric socket interface on the first support 111 without distinguishing the position and the function of each interface.

The servo motors are controlled in a data bus mode, multiple paths of motors are connected to the same data bus, and the servo motors corresponding to address numbers on the bus can respond and execute instructions according to address information contained in data packets.

The electric line connector can adopt a flat cable socket, and can also adopt other types of connectors such as an aviation plug and the like to realize the quick connection and disconnection of cables. The pin number of the connector is not limited to 9 pins, and the multiple LED light sources can adopt a common cathode/common anode or a group of independent cathode/anode pins of each light source according to the rated current of each pin of the connector. The pins used by the servo motor can also adopt a single-pin data pin (TTL signal control mode) or a 2-pin data pin (RS485 signal control mode) according to the type of the data bus, and other pins can be reserved for the expansion of subsequent functions, which is not limited by the disclosure.

According to the embodiment of the present disclosure, the culture dish rack fixing bracket 1 further includes: a rotary platform 14; the support body 11 is fixedly connected with the rotating platform 14, the support body 11 further includes second supports 112, the second supports 112 are radially distributed, and the end portion of the second support 112 extending out of the rotating platform 14 is fixed with the first support 111.

The culture dish frame fixed bolster that this disclosed embodiment provided, under rotary platform's drive, different culture dish framves can aim at the imaging device (for example CCD camera or CMOS camera) that fixed position set up respectively, and then can carry out the imaging analysis to the biological sample in a plurality of culture dish framves simultaneously conveniently, have improved the efficiency of sample analysis.

According to an embodiment of the present disclosure, the rotary platform 14 includes: a base 141, a flange 142 fixed on the base 141, a baffle 143 and a photoelectric limit switch 144 fixed on the base 141;

the blocking piece 143 is fixed at the gap of the flange 142, and the height of the blocking piece 143 is matched with the height of the photoelectric limit switch 144; the blocking plate 143 has a notch 1431, and the optical signal of the optoelectronic limit switch 144 can pass through the notch 1431.

In the mode of the embodiment of the present disclosure, the photoelectric limit switch 144 is used to calibrate the zero position of the rotation platform 14, when the rotation platform 14 rotates, the blocking sheet 143 can block the optical signal of the photoelectric limit switch 144, the optical signal at the opening 1431 can pass through, and calibration of the zero position can be realized by detecting the optical signal, so that the culture dish rack can be aligned to the imaging device.

An exemplary calibration method is as follows:

a) rotating counterclockwise at a speed of about 25 °/s until the photoelectric limit switch signal is turned on to stop moving;

b) rotating clockwise at the speed of about 10 degrees/s until the photoelectric limit switch signal is disconnected and stopping moving;

c) rotating counterclockwise at a speed of about 0.1 °/s until the photoelectric limit switch signal is turned on to stop moving;

d) the current position is set to zero by rotating 1 ° clockwise at the normal driving speed.

The zero point position is calibrated by the calibration method, so that the imaging equipment can accurately position the biological sample in the culture dish frame, and the analysis precision of the biological sample can be improved.

One of ordinary skill in the art will appreciate that other clockwise and counterclockwise rotation modes and rotation speeds may be used to achieve the calibrated zero position, and the disclosure is not limited thereto.

According to this disclosed embodiment, culture dish frame fixed bolster still includes: and corner reinforcing blocks 113 arranged between the adjacent second brackets 112 for reinforcing the bracket body 11.

In the manner of the disclosed embodiment, the corner reinforcing block 113 is disposed at a position of the first bracket 111 close to the second bracket 112, ensuring the stability of the bracket body 20 during rotation.

According to the embodiment of the present disclosure, still be provided with electric socket interface 13 on the first support 111, culture dish frame fixed bolster still includes: and a distribution board 15 fixed above the rotary platform 14, wherein a conductive slip ring 145 is provided inside the rotary platform 14, and cables connected to the electrical socket interface 13 are converged by the distribution board 15 and then electrically connected to the conductive slip ring 145.

In the mode of this disclosure, the quantity of electric socket interface 13 can correspond with the quantity of culture dish frame, be convenient for connect electric socket interface 13 and culture dish frame nearby, the cable that draws forth on different first supports 111 joins the back via branch line board 15, be connected with the inside electrically conductive sliding ring 145 electricity of rotary platform 14, guarantee the culture dish frame at the rotatory in-process that drives of first support 111, the cable can follow the rotatory in the lump of culture dish frame, can not take place the winding of cable, and conduct the electric signal of telecommunication to electric socket interface 13 by electrically conductive sliding ring 145 reliably.

Taking an 8-way branching circuit board as an example for explanation, as shown in fig. 3, the electrical socket interfaces 1 to 16 are electrical socket interfaces fixed on a plurality of first brackets 111, respectively, and the branching circuit board electrically connects 8 cables led in by the conductive slip ring with each electrical socket interface, respectively, wherein each electrical socket interface is electrically connected with 8 cables branched out by the 8-way branching circuit board.

According to the embodiment of the present disclosure, the top of the rotating platform 14 is provided with a mounting hole 146, and the second bracket 112 and the line distributing plate 15 are fixed through the mounting hole 146. Wherein the second bracket 112 is fixed between the line distributing plate 15 and the rotating platform 14. The rotary platform 14 is further provided with a central through hole 147, and after the cables connected to the electrical sockets 13 are converged by the distribution board 15, the cables are electrically connected to the conductive slip ring 145 through the central through hole 147.

According to an embodiment of the present disclosure, the mounting hole 146 includes: bracket mounting holes 1461 and distribution board mounting holes 1462; the bracket mounting holes 1461 and the distributing board mounting holes 1462 are arranged at intervals. The second brackets 112 are fixed through the bracket mounting holes 1461 to form radial distribution, the distribution board 15 is fixed above the rotary platform 14 through the distribution board mounting holes 1462, the second brackets 112 extend from the gaps between the vertical mounting pieces of the fixed distribution board 15 to the outside of the table top of the rotary platform 14 to form free ends, and the first brackets 111 are mounted at the free ends.

According to the embodiment of the present disclosure, the rack mounting holes 1461 are at least 2 sets, so that at least one set of adjacent first racks 111 is formed, so as to fix the culture dish rack in the space portion adjacent to the first racks 111.

According to the embodiment of the present disclosure, the bracket mounting holes 1461 are 8 groups, and an included angle between each group is 45 °, so as to form eight-direction main brackets, that is, the number of the first brackets 111 is 8, and the number of the adjacent first brackets 111 is 8, and the brackets are used for fixing culture dish racks.

One of ordinary skill in the art will appreciate that the bracket mounting holes 1461 may be provided in other sets and that the included angle between each set may be provided at other angles to form other than eight-way, main brackets. The present disclosure is not limited thereto.

In the mode of the embodiment of the present disclosure, each set of holder mounting holes 1461 may be used to fix a set of second holders 112, so that each of the first holders 111 and the second holders 112 is 8 sets, and after a culture dish rack is fixed on an adjacent first holder 111, for an imaging device in a fixed position, the culture dish rack may rotate in 8 directions, so that the culture dish rack in each direction aligns with the imaging device, and then imaging analysis of a biological sample is performed. It should be noted that a plurality of culture dish racks can be arranged on the first support 111 at different heights, and when performing imaging analysis, it is only necessary to adjust the height of the imaging device to image the biological sample in the culture dish rack, and the alignment position of the culture dish rack does not need to be adjusted.

In the mode of the embodiment of the present disclosure, the number of each group of bracket mounting holes 1461 may be 1 to 5, which provides support for fixing the bracket body 11, and the specific number may be flexibly adjusted as required, which is not limited by the present disclosure.

According to an embodiment of the present disclosure, the conductive slip ring 145 includes: a stator portion and a rotor portion; the rotor part is fixed on the table surface of the rotating platform 14; the stator portion is fixed to the base 141 of the rotary platform 14.

According to this disclosed embodiment, culture dish frame fixed bolster still includes: an air pump fixing plate 16, an air pump damping sleeve 17 and an air pump 18; the air pump fixing plate 16 is fixed above the distributing plate 15, a plurality of air pump damping sleeves 17 are fixed on the air pump fixing plate 16, and the air pump 18 is arranged inside the air pump damping sleeves 17; the air pump 18 is used for exchanging air inside and outside the culture dish rack.

In the mode of this disclosed embodiment, can go into the culture dish frame with gas pump such as air, oxygen, carbon dioxide, ethylene to keep unanimous in the air composition that realizes airtight culture dish and the incubator, be used for studying the growth state of plant under this experimental condition, thereby enough satisfied the demand of different experimental conditions.

According to this disclosed embodiment, culture dish frame fixed bolster still includes: an electrical equipment cover 19 fixed above the second bracket 112; an air pump pipe outlet 191 is formed in the electrical equipment cover 19, and an air pump pipe connected with the air pump 18 is communicated with the culture dish rack through the air pump pipe outlet 191.

According to this disclosed embodiment, culture dish frame fixed bolster still includes: and a pneumatic connector (not shown) connected to the air line of the air pump 19 and disposed on the first bracket 111.

In the mode of the embodiment of the present disclosure, the pneumatic connector is disposed on a side surface of the electrical socket connector 13, for example, a connector (a quick-insertion type, a quick-screwing type, or a pagoda type connector) for connecting a pneumatic hose may be installed, and the pneumatic connector is connected to the air pump 19 through a hose buried in the holder main body 11, and is connected to the air inlet of the culture dish holder through a hose.

According to this disclosed embodiment, culture dish frame fixed bolster still includes: and a liquid delivery joint (not shown in the figure) connected with a liquid pipeline of the conductive slip ring 145 and arranged on the first bracket 111 and used for supplying culture liquid into the culture dish rack.

In the mode of the embodiment of the present disclosure, the liquid delivery connector is disposed on a side surface of the electrical outlet port 13, and provides a culture solution for a biological sample in each culture dish rack. If the liquid pipeline is connected, the conductive slip ring 145 should be an electro-hydraulic type slip ring, and other elements of the liquid pipeline such as a culture solution storage bottle, a peristaltic pump and the like can be arranged on the air pump fixing plate 16, connected by a hose, and led into the stent main body 11 through a liquid delivery joint or the conductive slip ring 145.

One of ordinary skill in the art will appreciate that the culture fluid storage bottle, peristaltic pump, and fluid input connector may also be used to deliver fluids other than culture fluid to the culture dish rack, and the present disclosure is not limited thereto.

The culture dish frame fixed bolster that this disclosed embodiment provided, can be fixed in optics shock insulation platform, avoid outside vibrations to influence culture dish frame and imaging device's alignment precision, when utilizing imaging device to carry out the formation of image analysis to biological sample in the culture dish frame, the calibration of rotary platform zero point position need be carried out, after the calibration, can utilize step motor or directly drive motor control rotary platform's rotation rate, rotary platform drives the support main part rotatory, make the culture dish frame that needs the formation of image in each direction can aim at with the imaging device of fixed position, then utilize imaging device to carry out the analysis to the sample photo of shooting. The culture dish frame fixing support provided by the embodiment of the disclosure can fix a plurality of culture dish frames, so that imaging analysis can be performed on biological samples in the plurality of culture dish frames simultaneously, and the sample analysis flux is improved.

As shown in fig. 8, the present disclosure provides an incubator, the incubator 2 being fixable to an optical isolation platform, comprising: the culture dish rack comprises a culture dish rack fixing support 1 and a box body 20. The culture dish rack fixing bracket refers to the embodiment shown in fig. 1-7, and is not described in detail herein.

Trompil 21 has been seted up to the bottom of box 20, culture dish frame fixed bolster passes through trompil 21 is fixed in optics shock insulation platform to avoid influencing the stability when rotary platform 11 moves because of box 20 mechanical strength is not enough, and avoid on the direct conduction of vibrations of box 20 itself to culture dish frame fixed bolster. A thin rubber pad may be added between the opening 21 and the optical isolation platform to prevent the air tightness of the housing 20 from being damaged. An observation window 3 is installed on one side wall of the box body 20, the culture dish rack fixing support is rotated, and each culture dish rack A can be observed through the observation window 3.

According to the embodiment of the disclosure, the box body 20 is provided with the aviation socket panel 23, which is used for providing an electrical connection interface for the culture dish rack fixing support, and the electrical connection between the internal equipment of the box body 20 and the outside is realized through the aviation socket on the panel, so that the wiring position is light-tight and air-tight.

According to an embodiment of the present disclosure, the incubator 2 further comprises: the temperature control equipment is used for providing a constant temperature environment for the incubator; the temperature control apparatus includes: the compressor 24 is arranged outside the incubator and is connected with the box body 20 through a copper pipe, so that vibration generated during operation of the compressor is prevented from being transmitted to the culture dish frame fixing support, and the alignment precision of the biological sample shape imaging equipment and the culture dish frame is further improved.

The temperature control apparatus further includes: the other parts of the temperature control device, except the compressor 24, are arranged in the box body 20, and the box body 20 is also internally provided with a bottom strip-shaped air outlet 25, so that after the door of the box body 20 is closed, air cooled or heated by the temperature control device is blown out from the bottom strip-shaped air outlet 25 and sucked back from the top circulating fan to maintain the constant temperature environment of the closed space in the box body 20.

According to the embodiment of the present disclosure, the box body 20 is provided with an air inlet 26 and an air outlet 27, and an electromagnetic valve located at the air outlet 27; the gas inlet 26 is used for communicating with a gas supply component, and the gas supply component is used for providing a gas environment in the incubator; the solenoid valve is used to control the opening and closing state of the exhaust port 27.

In the mode of the embodiment of the present disclosure, the air supply assembly communicated with the air inlet 26 may provide experimental environments such as air, oxygen, carbon dioxide, ethylene, etc. for the closed space in the box 20, and the air pump 19 may pump the air in the box 20 into the corresponding culture dish rack, so as to study the growth state of the biological sample in the experimental environment.

It should be noted that, in order to avoid the influence of the original gas in the box 20 on the experimental conditions (such as gas concentration), the electromagnetic valve is used to open the exhaust port 27, high-pressure air is introduced to quickly flush the gas in the box 20 and exhaust the gas out of the box 20, and then the electromagnetic valve is used to close the exhaust port 27, so that the gas satisfying the experimental conditions can be introduced into the box 20 through the gas supply assembly. After the door is closed, the interior of the box 20 is kept strictly airtight, but is not light-tight except for the observation window, and is kept airtight, when the electromagnetic valve at the exhaust port 27 is closed, the gas is introduced from the air inlet 26 to generate a small positive air pressure in the box, so that the pollution of the internal environment caused by the permeation of the external air from a possible gap generated by the production and manufacturing process problems can be prevented.

According to the embodiment of the disclosure, the imaging device outside the box 20 can shoot the biological sample through the observation window 22, the glass used in the observation window 22 adopts double-sided coating, and the light transmittance of the glass in visible light and infrared light bands is increased, so that the reflection of the infrared illumination light source outside the box 20 to the lens is reduced, and the visible light signal loss of the biological sample inside the box 20 when the sensor outside the box 20 is used for detection is reduced.

According to the embodiment of the present disclosure, the observation window 22 is provided with heating members around the observation window for preventing the surface of the observation window glass from frosting when the observation window glass is in an internal low-temperature environment.

As shown in fig. 9, the present disclosure provides a biological sample morphology imaging apparatus including: the culture dish rack comprises a culture dish rack fixing support 1, an incubator 2, an optical shock insulation platform 3 and an imaging device 4; the incubator is shown in FIG. 8 and will not be described in detail. The incubator 2 and the imaging device 4 are both fixed on the optical shock insulation platform 3.

The imaging device 4 is used for shooting the shape of the biological sample in the culture dish rack through the observation window.

According to the embodiment of the disclosure, the shooting component of the imaging device can move in the directions of an X axis, a Y axis and a Z axis in a three-dimensional space by taking the plane of the observation window as the direction of the Z axis in the three-dimensional space. Through the displacement of adjustment imaging device in X axle, Y axle, Z axle direction to shoot the subassembly through rotary platform with corresponding culture dish frame A rotation alignment, can be to being fixed in biological sample formation of image in a plurality of culture dish frame A on culture dish frame fixed bolster, then carry out the analysis of imaging, and need not to adjust the alignment position of culture dish frame. Also can image a plurality of plant samples in same culture dish frame A to can realize automatic focusing, thereby realize high flux formation of image.

The biological sample form imaging device provided by the embodiment of the disclosure can be used for analyzing the growth state of a plant sample, especially a plant seedling, realizes simultaneous shooting of a large number of plant seedling samples through the rotary culture dish frame fixing support, and improves the analysis flux. Moreover, the high-throughput imaging analysis is met, meanwhile, the conditions of temperature, gas component concentration, illumination and the like of the plant seedling culture environment in the culture dish frame can be flexibly adjusted, the growth change of the plant seedlings is observed under various experimental condition variables, and the functions of the device are greatly expanded.

The foregoing description is only exemplary of the preferred embodiments of the disclosure and is illustrative of the principles of the technology employed. It will be appreciated by those skilled in the art that the scope of protection covered by this disclosure is not limited to the particular combination of features described above, but also encompasses other arrangements formed by any combination of features described above or equivalents thereof without departing from the spirit of the invention. For example, the above features and (but not limited to) the features disclosed in this disclosure having similar functions are replaced with each other to form the technical solution.

Claims

1. The utility model provides a culture dish frame fixed bolster which characterized in that includes: the bracket comprises a bracket body and a bracket fixing piece; the bracket body comprises a plurality of first brackets arranged at intervals, and at least one bracket position fixing piece is fixed on each first bracket; the rack position fixing piece comprises two rows of mounting positions, and the adjacent mounting positions on the rack position fixing piece of the adjacent two first supports are used for fixing a culture dish rack.

2. The culture dish holder fixing bracket according to claim 1, wherein the holder fixing member comprises: the bracket comprises a bracket fixing part, a first frame position part and a second frame position part which are arranged in pair; the first frame position part and the second frame position part are respectively positioned at two sides of the bracket fixing part; the first frame position part and the second frame position part are provided with at least two mounting holes; the mounting holes form a row of mounting positions.

3. The culture dish holder fixing bracket according to claim 2, wherein the first holder portion and the second holder portion are respectively arranged at an obtuse angle to the bracket fixing portion.

4. The culture dish holder fixing bracket according to claim 2, wherein the mounting hole is a through hole structure, a U-shaped groove or an L-shaped groove.

5. The culture dish holder fixing bracket according to any one of claims 2 to 4, wherein the holder fixing pieces are fixed at positions of different heights of the same first bracket; and/or

6. The culture dish rack fixing bracket according to any one of claims 2 to 4, wherein the heights of the mounting holes on two rack position fixing pieces for fixing the same culture dish rack are matched, and the heights of the two rack position fixing pieces are the same or different.

7. The culture dish holder fixing bracket according to claim 1, characterized in that,

the rack position fixing piece comprises a row of mounting positions, wherein each row of mounting positions of the rack position fixing piece consists of two mounting holes and is used for mounting a small culture dish rack on one side of the rack position fixing piece; or

8. The culture dish holder fixing bracket according to claim 1, wherein the first bracket is further provided with an electrical socket interface for electrically connecting with the culture dish holder.

9. The culture dish holder stationary bracket of claim 8, wherein the electrical socket interface comprises an electrical line connector with control pins.

10. The culture dish holder fixing bracket of claim 9, wherein the control pin in the electrical line connector comprises:

light source common cathode/common anode pin and/or

Overhead 1 st light source pin and/or

Overhead 2 nd-way light source pin and/or

Overhead 3 rd light source pin and/or

Overhead 4 th light source pin and/or

Side light source pin and/or

Servo motor power supply pin and/or

A servo motor ground pin and/or

Servo motor data bus pin.

11. The culture dish rack fixing bracket according to claim 1, wherein the first bracket is further provided with an air pump fixing plate, an air pump damping sleeve and an air pump; the air pump fixing plate is fixedly provided with a plurality of air pump damping sleeves, and the air pump is arranged inside the air pump damping sleeves; the air pump is used for exchanging air inside and outside the culture dish frame.

12. The culture dish holder fixing bracket according to claim 11, wherein the first bracket is further provided with a pneumatic connector connected with a gas pipeline of the gas pump for delivering gas into the culture dish holder.

13. The culture dish holder fixing bracket according to any one of claims 1 to 4 and 7 to 12, wherein the first bracket is further provided with a liquid delivery joint for delivering liquid into the culture dish holder.

14. The culture dish holder fixing bracket according to claim 1, further comprising:

the bracket body is fixedly connected with the rotating platform;

15. The culture dish holder stationary gantry of claim 14, wherein the rotating platform comprises: the device comprises a base, a flange fixed on the base, a baffle and a photoelectric limit switch fixed on the base;

16. The culture dish rack fixing support according to claim 14, wherein the first support is further provided with an electrical socket interface, the culture dish rack fixing support further comprising:

the distributing plate is fixed above the rotating platform;

17. The culture dish holder fixing bracket according to claim 16, wherein,

the top of the rotary platform is provided with a mounting hole, and the second bracket and the distributing board are fixed through the mounting hole; the second bracket is fixed between the distributing board and the rotating platform;

18. The culture dish holder fixing bracket according to claim 14, further comprising: an electrical equipment cover fixed above the second bracket; and an air pump pipe outlet is formed in the electrical equipment cover.

19. The culture dish holder fixing bracket according to claim 16, wherein the first bracket is further provided with a liquid delivery joint connected with a liquid pipeline of the conductive slip ring.

20. The culture dish holder stationary gantry of claim 19, wherein the fluid delivery fitting is disposed on a side of the electrical outlet interface.

21. The culture dish holder fixing bracket of claim 17, wherein the mounting hole comprises: the support mounting holes and the distributing plate mounting holes; the support mounting holes and the distributing plate mounting holes are arranged at intervals.

22. An incubator, wherein the incubator is internally provided with a culture dish holder holding rack according to any one of claims 1 to 21.

23. A biological specimen morphology imaging apparatus, comprising: the incubator and imaging apparatus of claim 22; the imaging device is used for shooting the form of the biological sample in the incubator.