CN213813319U - Automatic sample adding device for fluorescent quantitative PCR - Google Patents
Automatic sample adding device for fluorescent quantitative PCR Download PDFInfo
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- CN213813319U CN213813319U CN202022409645.4U CN202022409645U CN213813319U CN 213813319 U CN213813319 U CN 213813319U CN 202022409645 U CN202022409645 U CN 202022409645U CN 213813319 U CN213813319 U CN 213813319U
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Abstract
The utility model discloses an automatic sample adding device of fluorescence quantitative PCR, which comprises a base, wherein a movable frame and a guide rail are arranged on the base, the guide rail is fixedly connected on the base, the movable frame is connected with the guide rail and slides along the guide rail, a plurality of transverse or longitudinal chutes with scales are also arranged on the base, and each chute is provided with a clamping block which can move along the chute; the base is also provided with a test tube rack which is clamped by the clamping block to be fixed on the base; and a control system is also arranged on the periphery of the base. Through dispose mobilizable tight piece location of clamp on the base, guaranteed the precision of location when having simplified the structure, can also adapt to the test-tube rack of different specifications simultaneously, and adopt the adjustable shelf cooperation guide rail, cylinder control application of sample needle flexible when accomplishing the application of sample each side remove, further simplified the device structure, practiced thrift manufacturing cost.
Description
Technical Field
The utility model relates to a biomedical check out test set technical field especially relates to a fluorescence quantitative PCR's automatic application of sample device.
Background
The fluorescent quantitative PCR instrument is an experimental instrument for providing a stable and reliable temperature environment for in vitro simulation of DNA replication, and carries out real-time labeling and tracking on a PCR product through a fluorescent dye or a probe to realize rapid amplification and labeling detection of sample nucleic acid, so that the concentration of an original template is obtained through calculation by a standard curve method and the like. Specifically, when a nucleic acid sample is subjected to cleavage, annealing and extension to regenerate two double-stranded DNA molecules, an external light source is used for exciting a fluorescent dye to generate fluorescence and the amount of a currently circulating fluorescent signal is detected. The sample is switched between a high-temperature environment and a low-temperature environment, namely a single cycle of PCR, millions of amplification products can be obtained after about 40 cycles, the change of the fluorescence signal quantity directly reflects the change of nucleic acid substances, the original template concentration can be obtained through drawing a standard curve and calculating, and the relative quantitative effect is achieved.
Although the automatic sample adding device in the existing fluorescence quantitative PCR instrument does not need manual operation, the structure is complex, the manufacturing cost is higher, and errors are easily caused by dislocation when a sample is taken and put.
Therefore, in combination with the above-mentioned technical problems, there is a need to provide a new technical solution.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an automatic application of sample device of fluorescence quantitative PCR that the structure is simpler, the location is accurate and the suitability is high.
In order to solve the technical problem, the utility model provides an automatic application of sample device of fluorescence quantitative PCR, concrete technical scheme is as follows:
an automatic sample adding device for fluorescent quantitative PCR comprises a base, wherein a movable frame and a guide rail are arranged on the base, the guide rail is fixedly connected to the base, the movable frame is connected with the guide rail and slides along the guide rail, a plurality of transverse or longitudinal sliding grooves are also arranged on the base, scales are arranged on the sliding grooves, and clamping blocks capable of moving along the sliding grooves are arranged on each sliding groove; the base 1 is also provided with a test tube rack which is clamped by the clamping blocks to be fixed on the base; and a control system is also arranged on the periphery of the base.
Preferably, the automatic sample adding device for the fluorescence quantitative PCR further comprises a sample adding arm, one end of the sample adding arm is fixedly connected with the movable frame, and the other end of the sample adding arm is provided with a limiting block.
Preferably, be provided with the sliding block on the application of sample arm, the sliding block can be followed application of sample arm reciprocating motion and spacing by the stopper.
Preferably, be provided with cylinder and application of sample needle on the sliding block, the cylinder is the cylindric that link up, its perpendicular to base and with sliding block fixed connection, application of sample needle cover is established in the cylinder and through cylinder control along cylinder reciprocating motion.
Preferably, the control system is electrically connected with the movable frame.
Preferably, the control system is electrically connected to the slider.
The utility model discloses an automatic application of sample device of fluorescence quantitative PCR has following beneficial effect:
the utility model discloses an automatic application of sample device of fluorescence quantitative PCR through dispose mobilizable tight piece location of clamp on the base, has guaranteed the precision of location when having simplified the structure, can also adapt to the test-tube rack of different specifications simultaneously, and adopts the flexible each direction when accomplishing the application of sample of adjustable shelf cooperation guide rail, cylinder control application of sample needle to remove, has further simplified the device structure, has practiced thrift manufacturing cost.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the structure of the present invention;
fig. 2 is a schematic top view of the present invention.
Wherein, 1-a base; 11-a clamping block; 12-a chute; 2-a movable frame; 3-a guide rail; 4-a sample addition arm; 41-sliding block; 42-syringe; 43-a limiting block; 5-a sample adding needle; 6-test tube; 7-test tube rack.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Examples
Referring to fig. 1 and fig. 2, an automatic sample adding device for fluorescence quantitative PCR includes a base 1, and is characterized in that: the base 1 is provided with a movable frame 2 and a guide rail 3, the guide rail 3 is fixedly connected to the base 1, the movable frame 2 is connected with the guide rail 3 and slides along the guide rail 3, the base 1 is also provided with a plurality of transverse or longitudinal sliding grooves 12 with scales, and each sliding groove 12 is provided with a clamping block 11 capable of moving along the sliding groove; the base 1 is also provided with a test tube rack 7, the test tube rack 7 is clamped by the clamping block 11 to be fixed on the base 1, and the clamping block 11 can be moved to the corresponding scale of the sliding groove 12 in advance according to the test tube racks 7 with different specifications; a control system is also arranged at the periphery of the base 1. Fluorescence quantitative PCR's automatic application of sample device still includes application of sample arm 4, and application of sample arm 4's one end and adjustable shelf 2 fixed connection, the other end are provided with stopper 43. Be provided with sliding block 41 on application of sample arm 4, sliding block 41 can be followed application of sample arm 4 reciprocating motion and spacing by stopper 43. Be provided with cylinder 42 and application of sample needle 5 on the sliding block 41, cylinder 42 is the cylindric that link up, and its perpendicular to base 1 just with sliding block 41 fixed connection, and application of sample needle 5 covers establishes in cylinder 42 and passes through cylinder control along cylinder 42 reciprocating motion. The control system is electrically connected with the movable frame 2 and the slide block 41.
Use the utility model discloses a during fluorescence quantitative PCR's automatic application of sample device, at first take out test-tube rack 7 and fix test tube 6 in placing the cave of test-tube rack 7, place test-tube rack 7 on base 1 again, remove to press from both sides tight piece 11 and press from both sides tight test-tube rack 7, make test tube 7 fix on base 1. Through setting up corresponding parameter in control system, control adjustable shelf 2 moves along guide rail 3, adjustable shelf 2 drives application of sample arm 4 and removes, make application of sample needle 5 be located the top of the test tube 6 that is equipped with the sample, control system control sliding block 41 moves simultaneously, make application of sample needle 5's syringe needle aim at test tube 6, application of sample needle 5 moves down along cylinder 42 under the drive of cylinder and inserts in test tube 6 and extract the sample, application of sample needle 5 upward movement and move to waiting in the application of sample test tube under adjustable shelf 2 and sliding block 41's drive after the extraction, accomplish automatic application of sample.
The utility model has the advantages that: through dispose mobilizable tight piece location of clamp on the base, guaranteed the precision of location when having simplified the structure, can also adapt to the test-tube rack of different specifications simultaneously, and adopt the adjustable shelf cooperation guide rail, cylinder control application of sample needle flexible when accomplishing the application of sample each side remove, further simplified the device structure, practiced thrift manufacturing cost.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by one skilled in the art.
While embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications and changes may be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (7)
1. The utility model provides an automatic application of sample device of fluorescence quantitative PCR, includes base (1), its characterized in that: the base (1) is provided with a movable frame (2) and a guide rail (3), the guide rail (3) is fixedly connected to the base (1), the movable frame (2) is connected with the guide rail (3) and slides along the guide rail (3), the base (1) is also provided with a plurality of transverse and/or longitudinal sliding grooves (12), the sliding grooves (12) are provided with scales, and each sliding groove (12) is provided with a clamping block (11) capable of moving along the sliding groove; the base (1) is also provided with a test tube rack (7), and the test tube rack (7) is clamped by a clamping block (11) to be fixed on the base (1); the periphery of the base (1) is also provided with a control system.
2. The automatic sample adding device for fluorescence quantitative PCR according to claim 1, wherein: fluorescence quantitative PCR's automatic application of sample device still includes application of sample arm (4), the one end and adjustable shelf (2) fixed connection of application of sample arm (4), and the other end is provided with stopper (43).
3. The automatic sample adding device for fluorescence quantitative PCR according to claim 2, wherein: be provided with sliding block (41) on application of sample arm (4), sliding block (41) can be followed application of sample arm (4) reciprocating motion and spacing by stopper (43).
4. The automatic sample adding device for fluorescence quantitative PCR according to claim 3, wherein: be provided with cylinder (42) and application of sample needle (5) on sliding block (41), cylinder (42) are the cylindric that link up, its perpendicular to base (1) and with sliding block (41) fixed connection, application of sample needle (5) cover is established in cylinder (42) and is followed cylinder (42) reciprocating motion through cylinder control.
5. The automatic sample adding device for fluorescence quantitative PCR according to claim 1, wherein: the control system is electrically connected with the movable frame (2).
6. The automatic sample adding device for fluorescence quantitative PCR according to claim 3, wherein: the control system is electrically connected with the sliding block (41).
7. The automatic sample adding device for fluorescence quantitative PCR according to claim 1, wherein: the clamping block (11) is controlled by a screw rod to move transversely and/or longitudinally along the base (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022409645.4U CN213813319U (en) | 2020-10-27 | 2020-10-27 | Automatic sample adding device for fluorescent quantitative PCR |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022409645.4U CN213813319U (en) | 2020-10-27 | 2020-10-27 | Automatic sample adding device for fluorescent quantitative PCR |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213813319U true CN213813319U (en) | 2021-07-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022409645.4U Active CN213813319U (en) | 2020-10-27 | 2020-10-27 | Automatic sample adding device for fluorescent quantitative PCR |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213813319U (en) |
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2020
- 2020-10-27 CN CN202022409645.4U patent/CN213813319U/en active Active
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