CN213708376U - Constant temperature incubator of microorganism - Google Patents

Constant temperature incubator of microorganism Download PDF

Info

Publication number
CN213708376U
CN213708376U CN202021904466.1U CN202021904466U CN213708376U CN 213708376 U CN213708376 U CN 213708376U CN 202021904466 U CN202021904466 U CN 202021904466U CN 213708376 U CN213708376 U CN 213708376U
Authority
CN
China
Prior art keywords
culture
pure water
vacuum insulation
reverse osmosis
groups
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN202021904466.1U
Other languages
Chinese (zh)
Inventor
杨书锋
赵延峰
朱千相
潘丽娟
郭振立
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henan Jiuyuquan Food Co ltd
Original Assignee
Henan Jiuyuquan Food Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henan Jiuyuquan Food Co ltd filed Critical Henan Jiuyuquan Food Co ltd
Priority to CN202021904466.1U priority Critical patent/CN213708376U/en
Application granted granted Critical
Publication of CN213708376U publication Critical patent/CN213708376U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a constant temperature incubator of microorganism, including fuselage body and removal base, the inside top fixed mounting of fuselage body has the heating panel, the heating panel bottom is equipped with control mechanism. The utility model discloses a, the rotation axis has been adopted, the bracing piece, the culture dish brace table, the erlenmeyer flask brace table, the oscillation culture mechanism, reverse osmosis pure water mechanism and storing frame, before in-service use, can obtain the required utensil of cultivation from storing frame department, when beginning to cultivate the operation, can obtain the pure water through reverse osmosis pure water mechanism, carry out the allotment of microorganism nutrient solution and the regulation of the required humidity of microorganism, it rotates through the pulling rotation axis after the allotment finishes, make culture dish brace table and erlenmeyer flask brace table outwards or inside migration, make things convenient for culture apparatus's dress to get, the oscillation culture mechanism can carry out the oscillation culture, satisfy the cultivation demand of different microorganisms, it is various that the device wholly has the cultivation form, the function is various, load and unload nimble advantage.

Description

Constant temperature incubator of microorganism
Technical Field
The utility model relates to a microbial cultivation case technical field particularly, relates to a constant temperature incubator of microorganism.
Background
The microbial culture refers to the rapid growth and propagation of some (species) of microorganisms by means of artificially prepared culture media and artificially created culture conditions (such as culture temperature and the like), and is called microbial culture.
The operation of basic cultivation can only be carried out to current incubator most, consequently leads to cultivateing form and device function more singleness, and current equipment adopts the integral type heating to adjust the temperature mostly moreover, can't carry out the concurrent culture to the microorganism that demand temperature is different to current equipment, the device removes not very conveniently, and the business turn over of microorganism culture apparatus is not very nimble, has certain limitation in the use.
An effective solution to the problems in the related art has not been proposed yet.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model provides a to prior art not enough, the utility model provides a constant temperature incubator of microorganism possesses the advantage that the cultivation form is various, the equipment function is various, temperature independent control, it is convenient to remove, loading and unloading are nimble, and then solves the problem among the above-mentioned background art.
(II) technical scheme
For the advantage that realizes that above-mentioned cultivation form is various, the equipment function is various, temperature independent control, removal are convenient, loading and unloading are nimble, the utility model discloses a concrete technical scheme as follows:
a constant temperature incubator for microorganisms comprises a main body and a movable base, wherein a heat dissipation plate is fixedly arranged at the top end inside the main body, the bottom of the heat dissipation plate is provided with a control mechanism, the bottom of the control mechanism is fixedly provided with an ultraviolet germicidal lamp, a lamp strip is fixedly arranged in the middle of the bottom of the ultraviolet germicidal lamp, a temperature sensor and a humidity sensor are respectively arranged on two sides of the bottom of the ultraviolet germicidal lamp, the left side and the right side of the ultraviolet germicidal lamp are symmetrically connected with electric heating rings, one side of each electric heating ring is positioned on the inner surface of the machine body and is provided with a heat preservation layer, a rotating shaft is symmetrically arranged at the middle position of the two groups of electric heating rings below the lamp strip, one end of the rotating shaft is fixedly provided with a supporting rod, a culture dish supporting table and a conical flask supporting table are respectively installed at two ends of the supporting rod, and a first vacuum heat insulation plate is connected to the bottom of the electric heating ring.
Furthermore, a vibration culture mechanism is arranged at the middle position inside the machine body below the first vacuum heat insulation plate, the vibration culture mechanism is composed of a pressing plate, a knob, a foam frame, a vibration motor, a fixing rod, a telescopic rod and a panel, and a second vacuum heat insulation plate is fixedly connected to the bottom of the vibration culture mechanism.
Furthermore, a reverse osmosis pure water mechanism is installed on one side of the bottom of the second vacuum heat insulation plate, a storage rack is fixedly installed on one side of the reverse osmosis pure water mechanism, and the reverse osmosis pure water mechanism is composed of a reverse osmosis pure water machine, a guide pipe and an atomization nozzle.
Further, the bottom of the machine body is fixedly provided with a movable base, and two sides of the movable base are symmetrically provided with fixed supporting legs.
Furthermore, an explosion-proof glass door is hinged to one side of the machine body, and three groups of the explosion-proof glass doors are arranged.
Further, the inside one side of fuselage body runs through and is equipped with the ventilating fan, and the ventilating fan is equipped with four groups altogether, four groups the ventilating fan evenly sets up at rotation axis and vibration cultivation mechanism rear.
Furthermore, the ultraviolet sterilizing lamps, the lamp belts, the temperature sensors and the humidity sensors are all provided with three groups, and the ultraviolet sterilizing lamps, the lamp belts, the temperature sensors and the humidity sensors are respectively arranged at the bottoms of the control mechanism, the first vacuum heat insulation plate and the second vacuum heat insulation plate.
Furthermore, the control mechanism is composed of a temperature controller, a humidity controller, a control panel and a liquid crystal display screen.
Furthermore, four groups of electric heating rings and heat preservation layers are arranged, and the four groups of electric heating rings and heat preservation layers are uniformly distributed on two sides of the top ends of the first vacuum heat insulation plate and the second vacuum heat insulation plate.
(III) advantageous effects
Compared with the prior art, the utility model provides a constant temperature incubator of microorganism possesses following beneficial effect:
(1) the utility model adopts the rotating shaft, the supporting rod, the culture dish supporting platform, the conical flask supporting platform, the oscillation culture mechanism, the reverse osmosis pure water mechanism and the storage rack, before the actual use, the required apparatus for culture can be obtained from the storage rack, the cleaned apparatus can be stored through the storage rack after the culture is finished, the next use is convenient, when the culture operation is started, the pure water can be obtained through the reverse osmosis pure water mechanism, thereby the microorganism nutrient solution is prepared through the pure water and the humidity required by the microorganism is adjusted, the rotating shaft is pulled to rotate after the preparation is finished, the supporting rod, the culture dish supporting platform and the conical flask supporting platform are driven to move outwards or inwards, the loading and taking of the culture device are convenient, and the culture dish and the conical flask supporting platform are arranged, so the culture requirements of different microorganisms can be met, if the shaking culture is needed, the culture operation can be carried out by a shaking culture mechanism in the device, and the whole device has the advantages of various culture forms, various equipment functions and flexible assembly and disassembly.
(2) The utility model discloses a, the utility model discloses an, the electric heat ring has been adopted, the heat preservation, first vacuum heat insulating board, second vacuum heat insulating board and temperature sensor, in the use, detect the temperature in different spaces through temperature sensor, then come to control the device internal temperature through temperature controller, reach homothermal purpose, because first vacuum heat insulating board and second vacuum heat insulating board department all install the electric heat ring, heat preservation and temperature sensor, and the electric heat ring of two places is established alone, the separate heating, first vacuum heat insulating board and second vacuum heat insulating board can be isolated with the heat on upper strata, avoid passive intensification, the heat preservation can reduce the heat and dispel outward, can realize the effect of the different temperatures in different spaces, the device wholly has the advantage of temperature independent control.
(3) The utility model discloses a, adopted removal base and fixed leg, can firmly fix the device through fixed leg when the device uses, reinforcing stability can be through removing the base with the device fast migration when needing to remove, and the device wholly has the convenient advantage of removal.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of a thermostatic incubator for microorganisms according to the present invention;
FIG. 2 is a schematic structural view of a support table for a culture dish, a support table for a conical flask, a support rod and a rotation shaft according to the present invention;
fig. 3 is a schematic structural diagram of a culture dish support platform, a conical flask support platform, a shaking culture mechanism, a reverse osmosis pure water mechanism and a storage rack of the present invention.
In the figure:
1. a main body of the body; 2. a heat dissipation plate; 3. a control mechanism; 4. an ultraviolet germicidal lamp; 5. a light strip; 6. a temperature sensor; 7. an electric heating coil; 8. a heat-insulating layer; 9. a culture dish support table; 10. a ventilating fan; 11. a rotating shaft; 12. a flask support table; 13. a first vacuum insulation panel; 14. a shaking culture mechanism; 15. a reverse osmosis pure water mechanism; 16. a storage rack; 17. fixing the supporting legs; 18. moving the base; 19. a humidity sensor; 20. an explosion-proof glass door; 21. a support bar; 22. a second vacuum insulation panel.
Detailed Description
For further explanation of the embodiments, the drawings are provided as part of the disclosure and serve primarily to illustrate the embodiments and, together with the description, to explain the principles of operation of the embodiments, and to provide further explanation of the invention and advantages thereof, it will be understood by those skilled in the art that various other embodiments and advantages of the invention are possible, and that elements in the drawings are not to scale and that like reference numerals are generally used to designate like elements.
According to the utility model discloses an embodiment provides a constant temperature incubator of microorganism.
Referring to the drawings and the detailed description, the utility model will be further explained, as shown in fig. 1-3, a constant temperature incubator for microorganisms according to the embodiment of the utility model comprises a main body 1 and a movable base 18, a heat dissipation plate 2 is fixedly arranged at the top end inside the main body 1, a control mechanism 3 is arranged at the bottom of the heat dissipation plate 2, an ultraviolet sterilization lamp 4 is fixedly arranged at the bottom of the control mechanism 3, a lamp strip 5 is fixedly arranged at the middle position of the bottom of the ultraviolet sterilization lamp 4, a temperature sensor 6 and a humidity sensor 19 are respectively arranged at two sides of the bottom of the ultraviolet sterilization lamp 4, electric heating rings 7 are symmetrically connected at the left side and the right side of the ultraviolet sterilization lamp 4, a heat preservation layer 8 is arranged on the inner surface of the main body 1 at one side of the electric heating rings 7, a rotating shaft 11 is symmetrically arranged at, supporting rod 21 both ends are installed culture dish brace table 9 and erlenmeyer flask brace table 12 respectively, 7 bottoms of ring heater are connected with first vacuum insulation board 13, the device is pulling rotation axis 11 when using and is rotated, drive outside or the inside removal of supporting rod 21 and culture dish brace table 9 and erlenmeyer flask brace table 12, make things convenient for the dress of culture apparatus to get, and owing to set up culture dish brace table 9 and erlenmeyer flask brace table 12, so can satisfy the cultivation demand of different microorganisms, have the manifold advantage of cultivation form.
In one embodiment, a shaking culture mechanism 14 is arranged below the first vacuum insulation board 13 and in the middle of the inside of the machine body 1, the shaking culture mechanism 14 is composed of a pressing board, a knob, a foam frame, a shaking motor, a fixing rod, a telescopic rod and a panel, the bottom of the shaking culture mechanism 14 is fixedly connected with a second vacuum insulation board 22, and the foam frame can play a role in buffering and protecting a culture device.
In one embodiment, the reverse osmosis pure water mechanism 15 is installed at one side of the bottom of the second vacuum insulation panel 22, the storage rack 16 is fixedly installed at one side of the reverse osmosis pure water mechanism 15, the reverse osmosis pure water mechanism 15 is composed of a reverse osmosis pure water machine, a conduit and an atomizing nozzle, and the reverse osmosis pure water mechanism 15 can be used for obtaining pure water, so that the preparation of the microorganism nutrient solution and the adjustment of the required humidity of microorganisms are performed through the pure water.
In one embodiment, the bottom of the machine body 1 is fixedly provided with a movable base 18, two sides of the movable base 18 are symmetrically provided with fixed support legs 17, the fixed support legs 17 can fix the whole device to enhance the stability of the device, and the movable base 18 can transfer the device, so that the movable device has the advantage of convenient movement.
In one embodiment, the explosion-proof glass door 20 is hinged to one side of the machine body 1, three groups of explosion-proof glass doors 20 are arranged, and the three groups of explosion-proof glass doors 20 are used for independently sealing boxes with different functions, so that the influence among different microorganisms in the device is avoided, and the culture efficiency of the microorganisms is improved.
In one embodiment, the ventilation fans 10 are arranged on one side inside the machine body 1 in a penetrating manner, four groups of ventilation fans 10 are arranged on the four groups, the four groups of ventilation fans 10 are uniformly arranged behind the rotating shaft 11 and the oscillation culture mechanism 14, the ventilation fans 10 have the function of facilitating gas communication, gas generated by microbial respiration can be discharged, fresh gas is fed, and the problem that waste gas cannot be timely treated to influence microbial culture is solved.
In one embodiment, sterilamp 4, lamp area 5, temperature sensor 6 and humidity transducer 19 all are equipped with three groups, three groups of sterilamp 4, lamp area 5, temperature sensor 6 and humidity transducer 19 are installed respectively in control mechanism 3, first vacuum heat insulating board 13 and second vacuum heat insulating board 22 bottom, three groups of sterilamp 4 can cultivate the operation before the device and carry out germicidal treatment to the device inside, reduce the interference to the cultivation, lamp area 5 can throw light on the device inside, promote microbial growth and the personnel of being convenient for observe.
In one embodiment, the control mechanism 3 is composed of a temperature controller, a humidity controller, a control panel and a liquid crystal display, and the control mechanism 3 can play a role in controlling the overall operation of the device.
In one embodiment, the electric heating rings 7 and the heat preservation layer 8 are provided with four groups, the four groups of electric heating rings 7 and the heat preservation layer 8 are uniformly distributed on two sides of the top ends of the first vacuum heat insulation plate 13 and the second vacuum heat insulation plate 22, the electric heating rings at the two positions are independently arranged and are independently heated, the first vacuum heat insulation plate 13 and the second vacuum heat insulation plate 22 can isolate the heat of the upper layer, passive temperature rise is avoided, the heat preservation layer 8 can reduce the heat to be dissipated, the effect of different temperatures in different spaces can be realized, and the whole device has the advantage of independent temperature control.
The working principle is as follows: when the device is used, the device can be firmly fixed through the fixed supporting legs 17, the stability is enhanced, then the ultraviolet germicidal lamp 4 is turned on to sterilize the inside of the device, the subsequent cultivation is prevented from being influenced, after the sterilization is finished, the required apparatus for cultivation can be obtained from the storage rack 6, the cleaned apparatus can be stored through the storage rack 6 when the cultivation is finished, the next use is facilitated, when the cultivation operation is started, the pure water can be obtained through the reverse osmosis pure water mechanism 15, so that the microbial nutrient solution can be prepared and the humidity required by the microorganisms can be adjusted through the pure water, after the preparation is finished, the rotating shaft 11 is pulled to rotate, the supporting rod 21, the culture dish supporting platform 9 and the conical flask supporting platform 12 are driven to move outwards or inwards, the assembly and the disassembly of the cultivation device are facilitated, and due to the arrangement of the culture dish supporting platform 9 and the conical flask supporting, therefore, the culture requirements of different microorganisms can be met, and if the vibration culture is needed, the culture operation can also be carried out through the vibration culture mechanism 14 in the device, the whole device has the advantages of various culture forms, various equipment functions and flexible loading and unloading, the temperature in different spaces can be detected through the temperature sensor 6, the internal temperature of the device can be controlled through the control mechanism 3, the purpose of constant temperature is achieved, in addition, because the electric heating rings 7 are respectively arranged at the first vacuum heat-insulating plate 13 and the second vacuum heat-insulating plate 22, the heat-insulating layer 8 and the temperature sensor 6 are respectively arranged, the electric heating rings 7 at the two positions are respectively arranged and are respectively heated, the first vacuum heat-insulating plate 13 and the second vacuum heat-insulating plate 22 can isolate the heat at the upper layer, the passive temperature rise is avoided, the heat-insulating layer 8 can reduce the heat dissipation, and the effects of different, the device is whole to have the advantage of temperature independent control, designs very humanized, and when temperature regulation was suitable, culture apparatus laid when accomplishing, can close explosion-proof glass door 20, avoids the microorganism in the air to produce harmful effects to the cultivation, if need change the position this moment, just can shift fast through removing base 18, and the device has the convenient advantage of removal.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "disposed," "connected," "fixed," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (9)

1. The utility model provides a constant temperature incubator of microorganism, includes fuselage body (1) and removes base (18), its characterized in that, the inside top fixed mounting of fuselage body (1) has heating panel (2), heating panel (2) bottom is equipped with control mechanism (3), control mechanism (3) bottom fixed mounting has sterilamp (4), sterilamp (4) bottom intermediate position fixed mounting has lamp area (5), temperature sensor (6) and humidity transducer (19) are installed respectively to sterilamp (4) bottom both sides, sterilamp (4) left and right sides symmetric connection has ring heater (7), ring heater (7) one side is located fuselage body (1) inside surface mounting has heat preservation (8), rotation axis (11) are installed to the symmetry of lamp area (5) below being located two sets of ring heater (7) intermediate position, the utility model discloses a culture dish, including rotation axis (11), bracing piece (21), culture dish brace table (9) and erlenmeyer flask brace table (12) are installed respectively to bracing piece (21) both ends, electric heat ring (7) bottom is connected with first vacuum insulation board (13).
2. The incubator according to claim 1, wherein a shaking culture mechanism (14) is provided below the first vacuum insulation board (13) at a middle position inside the main body (1), the shaking culture mechanism (14) is composed of a pressing board, a knob, a foam frame, a shaking motor, a fixing rod, a telescopic rod and a panel, and a second vacuum insulation board (22) is fixedly connected to the bottom of the shaking culture mechanism (14).
3. The incubator according to claim 2, wherein the reverse osmosis pure water mechanism (15) is installed on one side of the bottom of the second vacuum insulation board (22), the storage rack (16) is fixedly installed on one side of the reverse osmosis pure water mechanism (15), and the reverse osmosis pure water mechanism (15) comprises a reverse osmosis pure water machine, a conduit and an atomizing nozzle.
4. The incubator according to claim 1, wherein a movable base (18) is fixedly installed at the bottom of the body (1), and fixed legs (17) are symmetrically arranged on two sides of the movable base (18).
5. The incubator according to claim 1, wherein three groups of explosion-proof glass doors (20) are hinged to one side of the main body (1).
6. The incubator according to claim 1, wherein four sets of ventilating fans (10) are provided in the body (1) at one side of the interior, and the four sets of ventilating fans (10) are uniformly provided behind the rotating shaft (11) and the shaking culture mechanism (14).
7. The incubator according to claim 1, wherein the sterilamp (4), the lamp strip (5), the temperature sensor (6) and the humidity sensor (19) are provided with three groups, and the three groups of the sterilamp (4), the lamp strip (5), the temperature sensor (6) and the humidity sensor (19) are respectively arranged at the bottom of the control mechanism (3), the first vacuum insulation board (13) and the second vacuum insulation board (22).
8. The incubator according to claim 1, wherein said control means (3) is composed of a temperature controller, a humidity controller, a control panel and a liquid crystal display.
9. The incubator according to claim 1, wherein four groups of said electric heating rings (7) and insulating layers (8) are provided, and four groups of said electric heating rings (7) and insulating layers (8) are uniformly distributed on both sides of the top ends of the first vacuum insulation panel (13) and the second vacuum insulation panel (22).
CN202021904466.1U 2020-09-03 2020-09-03 Constant temperature incubator of microorganism Expired - Fee Related CN213708376U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021904466.1U CN213708376U (en) 2020-09-03 2020-09-03 Constant temperature incubator of microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021904466.1U CN213708376U (en) 2020-09-03 2020-09-03 Constant temperature incubator of microorganism

Publications (1)

Publication Number Publication Date
CN213708376U true CN213708376U (en) 2021-07-16

Family

ID=76792363

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021904466.1U Expired - Fee Related CN213708376U (en) 2020-09-03 2020-09-03 Constant temperature incubator of microorganism

Country Status (1)

Country Link
CN (1) CN213708376U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117586879A (en) * 2023-11-23 2024-02-23 一恒生命科学仪器(昆山)有限公司 Micro-vibration type incubator with cleaning function and use method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117586879A (en) * 2023-11-23 2024-02-23 一恒生命科学仪器(昆山)有限公司 Micro-vibration type incubator with cleaning function and use method thereof

Similar Documents

Publication Publication Date Title
US3002895A (en) Incubator-shaker apparatus
CN213708376U (en) Constant temperature incubator of microorganism
CN111334423A (en) Microbial incubator
CN107937248A (en) A kind of microbiological incubator
CN208517394U (en) A kind of microbial bacterial agent fermentation feeder
CN109526586A (en) A kind of full-automatic Pleurotus eryngii cultivation room humidification temperature regulating device and its application method
CN208898870U (en) A kind of clinical medicine medical treatment viable bacteria incubator
CN106554918A (en) A kind of microorganism cultivates integration apparatus
CN217908393U (en) Edible mushroom culture medium sterilization apparatus
CN110241000A (en) A kind of microbial bacteria culture apparatus
CN213416861U (en) Multifunctional microorganism incubator convenient to operate
CN208258355U (en) A kind of uniform dry heat sterilization processing equipment of seed
CN212199278U (en) Microbial incubator
KR200492879Y1 (en) Culture stand for natural circulation of drawer type
CN208182984U (en) A kind of biology laboratory microbial cultivation device
CN218689662U (en) Superclean bench
CN221777889U (en) Raw material preparation device for probiotic-carrying electrospun film
CN219772102U (en) Incubator is with constant temperature shaking table
CN109294900A (en) A kind of hatching apparatus of vaccine
CN212937285U (en) Breeding pig is constant temperature artificial containers for breeding
CN218941861U (en) Sterile dust-free culture auxiliary device
CN214962284U (en) Equipment for bread fermentation
CN218589392U (en) Sterilizer of microorganism culture medium
CN113916996B (en) Column incubator for gas chromatograph and gas chromatograph
CN215957677U (en) Insulation can for livestock breeding

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210716

CF01 Termination of patent right due to non-payment of annual fee