CN213680729U - Butt-joint structure of carbon dioxide incubator and isolator - Google Patents

Abstract

The utility model discloses a butt joint structure of a carbon dioxide incubator and an isolator, the incubator comprises an outer box and an inner box, when in butt joint, the inner flange ring and the outer flange ring are hermetically connected through a third inflatable sealing ring, a sterilization medium generation module is started to sterilize the sterilization space at the joint, then the connection between the butt joint outlet of the inner box and the connection between the outer flange door and the outer flange ring are disconnected, and the outer flange door and the inner box door can be opened to transmit a culture dish; and closing the inner box door and the outer flange door, enabling the inner box door to be connected with the butt joint outlet in a recovering mode through the first inflatable sealing ring, enabling the inner flange ring to be disconnected with the outer flange ring through the third inflatable sealing ring, separating the incubator from the isolator, and starting the electric heating element in the outer box in a recovering mode to enable the inner box to continue to be uniformly heated. The utility model discloses can ensure that the temperature is even in the incubator, stop including the inboard condensation that forms of chamber door effectively.

Description

Butt-joint structure of carbon dioxide incubator and isolator

Technical Field

The utility model relates to an aseptic transmission technology field, concretely relates to butt joint structure of carbon dioxide incubator and isolator.

Background

The research and development and production of cell drugs related to gene therapy, stem cell, immunocyte therapy, tissue engineering and the like have become an important direction for the future development of biological medicines. In the production of cell products, how to realize the sterility of the process is the key to the quality assurance. Since the sterility of the cell product is verified by the final sterility test to have disadvantages such as long time, low detection rate, etc., it is generally necessary to ensure the sterility of the cell product by aseptic manipulation of the process. In the production process of cell products and other aseptic drugs, primary cells are often required to be cultured in an incubator, which is required to maintain a constant culture temperature (37 ℃), a relative humidity of 95%, a carbon dioxide content of 5%, and a pH value of ph 7.2-7.4. The cells are firstly placed in a larger sterile isolator, then the culture box and the isolator realize sterile rapid butt joint, then the culture dish for inoculating the cells in the isolator is placed in the culture box, and then the culture box is sealed and separated from the isolator, thus the sterile culture of the cells in the culture box can be started.

In the prior art, incubators and isolators have typically been used to aseptically dock and transport plates using a structure such as that disclosed in U.S. Pat. No. US20170022468A1, entitled Modular Incubator. A modular incubator comprises an incubation station and a plurality of RTP butt joint type incubator modules, wherein outer boxes and inner boxes of the RTP butt joint type incubator modules are communicated with each other, beta-RTP main valves are installed on the outer boxes of the RTP butt joint type incubator modules and are in butt joint with alpha-RTP auxiliary valves installed on isolators, and therefore the incubator and the isolators are in sterile butt joint. The RTP docking incubator modules are not communicated with each other, thereby ensuring that each single incubator module is cultured under the sterile condition and cannot cross-contaminate each other.

However, such incubators still have the following drawbacks: first, due to the structural limitation, the beta-RTP door (i.e., the inner door) of the incubator cannot be provided with an electric heating element, so that the beta-RTP door of the incubator is always difficult to reach the set incubation temperature of 37 ℃, and is usually lower than 37 ℃, thereby causing poor uniformity of the temperature in the incubator. Because the relative humidity in the incubator is high (the closed environment is generally more than 95 percent), when the temperature of the inner side of the beta-RTP door is lower than the dew point temperature, condensation can be formed on the inner side of the beta-RTP door, and pollution is easy to occur; secondly, the butt joint outlet of the incubator is of a circular structure, the space utilization rate is low, and the requirement for the culture of a large-capacity/large-volume culture vessel cannot be met; moreover, the RTP depends on manual rotation for butt joint, so that the rotation resistance is large, the mechanical structure is complex, the manufacturing difficulty is large, and the cost is high; in addition, when incubator and isolator butt joint, realize sealed through the sealing strip usually to avoid receiving the pollution of outside air, the unable sterilization of sealing strip department has the pollution risk, and the sealing strip has the risk of ageing the revealing.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a first purpose is in order to solve the poor problem of the incasement temperature homogeneity that current RTP carbon dioxide incubator exists, provides a butt joint structure of carbon dioxide incubator and isolator, can ensure that the temperature is even in the incubator, stops including the inboard condensation that forms of chamber door effectively.

The second purpose of the utility model is to provide a butt joint structure of carbon dioxide incubator and isolator in order to solve the problem that the existing RTP carbon dioxide incubator exists and the isolator is poor in connection reliability, can ensure reliable connection and sealing between interior chamber door and the outer flange door to simple structure conveniently operates.

The third purpose of the utility model is that in order to solve the restriction that receives circular butt joint export that current RTP carbon dioxide incubator exists, space utilization is low, provides the butt joint structure of a carbon dioxide incubator and isolator, but furthest promotes the utilization ratio of incubator inner space, satisfies the cultivation of large capacity/bulky culture dish.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a butt-joint structure of a carbon dioxide incubator and an isolator is disclosed, the incubator comprises an outer box movably arranged on a moving vehicle and an inner box positioned in the outer box, one side of the outer box in the moving direction is provided with a rotatable outer box door, an auxiliary electric heating element is arranged in the outer box door, one side of the inner box corresponding to the outer box door is provided with a butt-joint outlet, the edge of the butt-joint outlet is provided with an inner flange ring protruding outwards, the inner box door is arranged in the inner flange ring, a first inflatable sealing ring is arranged between the inner flange ring and the inner box door, one side of the isolator is provided with a transmission outlet, the edge of the transmission outlet is provided with an outer flange ring protruding outwards, the outer flange ring is provided with an outer flange door, a second inflatable sealing ring is arranged between the outer flange ring and the outer flange door, a third inflatable sealing ring is arranged outside the outer flange door in the outer flange, when the inner flange ring enters the outer flange ring, the outer flange ring is hermetically connected with the inner flange ring through a third inflatable sealing ring, a sterilization space is enclosed by the outer flange ring, the inner flange ring, the outer flange door and the inner box door, and the sterilization space is connected with the sterilization medium generation module and the sterilization medium decomposition module.

First, the incubator of the present invention is movably disposed on the moving vehicle, thereby facilitating the rapid movement of the incubator and the docking with the isolator. In particular, the incubator includes an outer case and an inner case disposed in the outer case, that is, the incubator has a double-layered structure, and an auxiliary electric heating element is disposed in the outer case door, so that the inner case door can be heated by the auxiliary electric heating element to maintain substantially the same temperature as the inner case. That is to say, the culture dish and the inner box door in the inner box can keep the same culture temperature at this moment, thereby effectively avoiding the condensation phenomenon caused by the lower temperature of the inner box door, and avoiding the pollution of the cells in the culture dish caused by condensation. In addition, the incubator with the double-layer structure is also favorable for improving the sterile effect of the inner box.

It can be known that the inflatable sealing ring can realize effective sealing connection between two components when inflated and expanded, overcomes the adverse effect on sealing caused by manufacturing errors such as shape and size, and can conveniently realize the connection and positioning between the two components. When we deflate the inflatable sealing ring, the connection relationship between the two components can be conveniently released.

It should be noted that the sterilizing medium generating module of the present invention preferably uses gaseous hydrogen peroxide as the sterilizing agent filled into the sterilizing space, and the hydrogen peroxide has strong oxidizing property, so that the sterilizing space can be effectively sterilized. After the sterilization is finished, the sterilization medium decomposition module is started, so that residual hydrogen peroxide is decomposed, a sterile environment of a space is maintained through the efficient air filter, and the harm to operators is avoided.

When an operator extends a hand into the isolator through a glove port on the isolator and opens the outer flange door, the inner box door can be opened because the sterilization space is in an aseptic state at the moment, and aseptic butt joint of the inner box and the isolator and aseptic transmission of a culture dish are ensured.

Preferably, one side of the outer flange door is rotatably connected to the outer flange ring, and one side of the inner door is rotatably connected to the inner flange ring.

The outer flange door connected to the outer flange ring can be conveniently opened towards the inner side of the isolator, and the inner box door connected to the inner flange ring in a rotating mode can be conveniently opened outwards, so that the transmission outlet of the isolator is in butt joint with the butt joint outlet of the inner box, and transmission of a culture dish is facilitated.

Preferably, a turbulent fan is arranged in the inner box.

The turbulent fan can make the air in the inner box form turbulent flow, thereby being beneficial to the uniform temperature in the inner box.

Preferably, the incubator further comprises a first electromagnetic lock capable of locking the incubator and a second electromagnetic lock capable of locking the mobile vehicle, and a position sensor capable of sensing the position of the mobile vehicle is arranged outside the isolator.

The first electromagnetic lock locking the incubator securely positions the incubator on the carriage. When we move the locomotive to the outer flange door department that is close to the isolator, the position sensor that sets up in the isolator outside can the sensing locomotive's position to send position signal to control system, control system can start the second electromagnetic lock of locking locomotive and the locking locomotive, makes the locomotive keep the position fixed, then, control system opens the first electromagnetic lock of locking incubator, so that the butt joint of mobile incubator and isolator.

Particularly, the first electromagnetic lock and the second electromagnetic lock are opened and closed, so that the reliable positioning of the moving vehicle and the incubator is realized, meanwhile, the control system can accurately record parameters such as time and times when the incubator is in butt joint with the isolator, and the follow-up and automatic control of the culture time and the like in the later period are facilitated.

Preferably, the isolator lateral wall is equipped with the guide way, is equipped with in the guide way the second electromagnetic lock, the locomotive is equipped with the guide post in corresponding the guide way position, when the locomotive removes to the outer flange door department that is close to the isolator, the guide post gets into the guide way in, second electromagnetic lock locking guide post.

When we move the locomotive to the outer flange door department that is close to the isolator, the guide post on the locomotive can peg graft in the guide way of isolator lateral wall to make the incubator on locomotive and the locomotive accurately counterpoint with the isolator, and the second electromagnetic lock that sets up in the guide way then can conveniently lock the guide post, and then realize the reliable location of locomotive. That is to say, the cooperation of guide post and guide way both can realize the accurate quick location of locomotive relative isolator, can make things convenient for the locking of locomotive again.

Preferably, the butt joint outlet is rectangular, and a plurality of layers of shelves for placing culture dishes are arranged in the inner box.

The utility model discloses set the butt joint export to the rectangle to set up the multilayer in the inner box and be used for placing the shelf of culture dish, and make things convenient for the transmission of culture dish.

Preferably, the inner side bottom wall of the inner box, which is close to the inner box door, is provided with a water receiving disc, the bottom wall of the water receiving disc is connected with a radiator positioned between the outer box and the inner box, and the radiator is communicated with the outside air through a radiating pipeline.

Because the diapire of water collector is connected with the radiator, and the radiator passes through heat dissipation pipeline and outside air intercommunication, consequently in the inside of inner box, the water collector can become the minimum temperature department, and at this moment, the steam in the inner box air can condense preferentially and form the comdenstion water in the water collector. That is to say, when the environment temperature is low and the temperature of the inner box is not enough to keep the temperature of the water pan above the dew point temperature, the inner box only condenses at the water pan to form condensed water, thereby thoroughly avoiding the pollution caused by condensation at other parts of the inner box.

Preferably, an automatic sterilization system is arranged between the inner box and the outer box and comprises a vaporized hydrogen peroxide generation module and a vaporized hydrogen peroxide decomposition module, the output ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of the output pipeline through electric control valves, the input ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of the input pipeline through electric control valves, the other end of the input pipeline is communicated with the bottom wall of the inner box, the other end of the output pipeline is communicated with the top wall of the inner box, a circulating fan and a filter are arranged on the input pipeline, a filter is arranged on the output pipeline, and a hydrogen peroxide concentration sensor is arranged in the inner box.

The utility model discloses be equipped with automatic sterilization system between inner box and outer container, consequently, when we close the automatically controlled valve of decomposing the input of module, output with the vaporized hydrogen peroxide, start vaporized hydrogen peroxide generation module and circulating fan, circulating fan can carry out sterilization in the inner box through output pipeline with the vaporized hydrogen peroxide that vaporized hydrogen peroxide generation module generated, and output pipeline and the filter on the input pipeline then effectively purify the inner box air to A level environment, maintain the aseptic nature of incasement. When the hydrogen peroxide concentration in the inner box detected by the hydrogen peroxide concentration sensor reaches a set value, the vaporized hydrogen peroxide generation module continues to work for a period of time, and at the moment, the inner box maintains the hydrogen peroxide sterilization concentration. When the sterilization is finished, the electric control valves connected with the input end and the output end of the vaporized hydrogen peroxide generation module are closed, meanwhile, the vaporized hydrogen peroxide generation module stops working, and the electric control valves connected with the input end and the output end of the vaporized hydrogen peroxide decomposition module are opened, so that the sterilization loop is closed, the decomposition loop is opened, and then the inner box is decomposed by hydrogen peroxide. When the hydrogen peroxide concentration in the inner box detected by the hydrogen peroxide concentration sensor is zero, the electric control valve connected with the input end and the output end of the vaporized hydrogen peroxide decomposition module is closed, so that the automatic sterilization and disinfection of the inner box are completed.

Preferably, the mobile vehicle is provided with a carbon dioxide gas bottle communicated with the inner box through a pipeline and a mobile power supply for supplying power to the incubator.

The carbon dioxide gas cylinder can make the incubator maintain the carbon dioxide content of invariant, and portable power source makes things convenient for the removal of locomotive on the one hand, and on the other hand can make the incubator maintain the on-state all the time.

Therefore, the utility model discloses following beneficial effect has: the heating of the outer box door of the incubator enables the temperature of the inner box door to reach the temperature in the incubator, so that the temperature in the incubator can be ensured to be uniform, the condensation formed on the inner side of the inner box door is effectively avoided, and the pollution is prevented. When the incubator is connected with the isolator, reliable connection and sealing between the inner box door of the incubator and the outer flange door of the isolator can be ensured. The butt joint export of incubator is the rectangle structure, can place multilayer culture dish shelf, and space utilization is high. In addition, still can carry out on-line measuring to incubator body leakage rate and leakproofness, guarantee the integrality and the aseptic nature of incubator. The incubator opens the door and possesses automated inspection and electronic record, conveniently traces back. The whole butt joint process of incubator and isolator adopts the electronic interlocking structure, guarantees whole operation process's sterility and reliability.

Drawings

FIG. 1 is a schematic view of the incubator and the moving cart according to the present invention.

FIG. 2 is a schematic view of a structure of the incubator.

FIG. 3 is a side sectional view of the incubator.

Fig. 4 is a partial structural view of the separator.

FIG. 5 is a schematic view of a docking structure of the incubator and the isolator.

Fig. 6 is a schematic view of a connection structure of the guide post and the guide groove.

FIG. 7 is another schematic view of the incubator and the carriage of the present invention.

In the figure: 1. the device comprises an incubator 11, an outer box 111, an outer box door 12, an inner box 121, a butt joint outlet 122, an inner flange ring 123, an inner box door 124, a shelf 125, a turbulent fan 13, a first inflatable sealing ring 14, a second inflatable sealing ring 15, a third inflatable sealing ring 17, a first electromagnetic lock 18, a second electromagnetic lock 181, a locking head 2, a moving vehicle 21, a guide column 211, a locking hole 3, an isolator 31, a transmission outlet 32, an outer flange ring 33, an outer flange door 34, a guide groove 4, a carbon dioxide gas cylinder 5, a mobile power supply 6, a sterilization medium generation module 7, a sterilization medium decomposition module 8, a water pan 81, a radiator 91, a vaporized hydrogen peroxide generation module 92, a vaporized hydrogen peroxide decomposition module 93, an electronic control valve 94, an output pipeline 95, an input pipeline 96, a circulating fan 97, a first electromagnetic lock, a second electromagnetic lock 181, a first, And (3) a filter.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and the detailed description.

As shown in FIGS. 1, 2 and 3, a docking structure of a carbon dioxide incubator and an isolator, wherein the incubator 1 includes an outer case 11 movably installed on a mobile vehicle 2 and an inner case 12 provided in the outer case, thereby forming the incubator as a double structure. For convenience of description, in this embodiment, the side of the incubator that is close to the isolator when the incubator moves on the moving vehicle is referred to as the front side, and the side that is away from the isolator is referred to as the rear side. The outer box is provided with an outer box door 111 which can be rotated and opened outward at the front side in the moving direction, an electric heating element for maintaining the inner box at a culture temperature is provided in the inner box, and an auxiliary electric heating element (not shown) is provided in the outer box door. The inner box is provided with a butt joint outlet 121 at the front side corresponding to the outer box door, an inner flange ring 122 protruding outwards is arranged at the edge of the butt joint outlet, an inner box door 123 is arranged in the inner flange ring, and a first inflatable sealing ring 13 is arranged between the inner flange ring and the inner box door so as to realize the sealing connection between the inner box door and the butt joint outlet.

In addition, as shown in fig. 4, a delivery outlet 31 is provided at one side of the separator 3, an outer flange ring 32 protruding outward is provided at an edge of the delivery outlet, an outer flange door 33 is provided in the outer flange ring, and a second air packing 14 is provided between the outer flange ring and the outer flange door, so as to achieve a sealed connection between the outer flange ring and the outer flange door. In addition, a third inflatable sealing ring 15 is arranged on the outer side of the outer flange door in the outer flange ring. Of course, a corresponding control system is required to control the actions of each device and component.

When the incubator is required to be in butt joint with the isolator, the outer box door is opened for more than 180 degrees, the preferred value is 270 degrees, so that the inner flange ring and the inner box door are exposed, and then the moving vehicle is moved to be close to the outer flange door of the isolator and is positioned. Of course, the first and second pneumatic sealing rings should be in a pneumatic state at this time, so that the inner box door is connected with the inner flange ring in a sealing manner, and the outer flange door is connected with the outer flange ring in a sealing manner. Next, as shown in fig. 5, the incubator is moved toward one side of the outer flange door, so that the inner flange ring enters the outer flange ring, and at this time, the third inflatable sealing ring is inflated by the control system to hermetically connect the inner flange ring and the outer flange ring, so that a closed sterilization space is formed among the outer flange ring, the inner flange ring, the outer flange door and the inner chamber door. At this time, the sterilization medium generation module 6 connected to the outer flange ring is started to fill the sterilization space with the oxidizing sterilizing agent such as hydrogen peroxide to sterilize the sterilization space, and then the sterilization medium decomposition module 7 connected to the outer flange ring is started to decompose the residual hydrogen peroxide sterilizing agent, so that the sterile environment of the space is maintained through the high-efficiency air filter, and the adverse effect of the residual sterilizing agent on operators is effectively avoided. And then deflating the first inflatable sealing ring, so that the connection between the inner box door and the butt joint outlet is disconnected, deflating the second inflatable sealing ring, and disconnecting the connection between the outer flange door and the outer flange ring. Then, an operator can stretch hands into the isolator through a glove port on the isolator, and open the outer flange door inwards, at the moment, the inner box door is exposed, the operator can continue to open the inner box door, then place a culture dish prepared in the isolator on the shelf 124 of the inner box after penetrating through the delivery outlet and the butt joint outlet, close the inner box door and the outer flange door, inflate the first inflatable sealing ring to enable the inner box door to be in sealed connection with the butt joint outlet, and inflate the second inflatable sealing ring to enable the outer flange door to be in sealed connection with the outer flange ring. After the transfer of the culture dish is completed, the third inflatable sealing ring can be deflated, so that the sealing connection between the inner flange ring and the outer flange ring is broken, the culture box is moved backwards, the culture box is separated from the isolator, and then the outer box door is closed. At this time, the auxiliary electric heating element in the outer box door is opened again, so that the inner box door can be continuously and uniformly heated, and the inner box door and the inner box are maintained at the same culture temperature. The electric heating element of the inner box is kept running all the time during the docking process of the incubator and the isolator.

Because the incubator includes outer container and the inner box that is located the outer container, consequently, can make the inner box door receive the heating of auxiliary electric heating element and maintain higher temperature to avoid effectively producing the condensation phenomenon because of inner box door department temperature is lower, stop the cell in the culture dish and be polluted because of the condensation.

It should be noted that, since the sterilization medium generation module delivers the gaseous hydrogen peroxide for sterilization, the sterilization medium decomposition module decomposes the hydrogen peroxide, which is not described in detail herein. In addition, for the convenience of operation, an operation opening is arranged on the isolator, and an isolation glove is connected to the operation opening in a sealing mode, so that a glove opening is formed. When operating personnel need operate isolator inside, can stretch into soft isolation gloves with the hand, can operate isolator inside, and isolation gloves can be isolated isolator inside and outside sealed, avoid isolator inside to receive the pollution. Since the basic structure of the isolator belongs to the prior art, which is out of the technical scope of the present application, it will not be described in detail herein.

Preferably, one side of the outer flange door is rotatably connected to the outer flange ring, and one side of the inner box door is also rotatably connected to the inner flange ring, so that the inner box door can be opened outwards relative to the inner box when the outer flange door is opened inwards, and conversely, the inner box door can be closed firstly and then the outer flange door is closed.

Further, a turbulent fan 125 may be disposed in the inner box to make the air in the inner box turbulent, thereby facilitating the temperature in the inner box to be uniform.

As a preferred scheme, the utility model discloses still including setting up the second electromagnetic lock 18 that is used for the locking locomotive on the isolator, setting up the first electromagnetic lock 17 that can lock the incubator on the locomotive, but the isolator outside sets up the first position sensor of sensing locomotive position. The first electromagnetic lock locks the incubator securely to the travel car.

When the moving vehicle moves to the position close to the outer flange door of the isolator, the first position sensor sends a position signal to the control system, the control system starts the second electromagnetic lock to lock the moving vehicle, so that the moving vehicle keeps the fixed position, and correspondingly opens the first electromagnetic lock of the incubator, so that the incubator can be moved and is in butt joint with the isolator.

Through opening and close of first, second electromagnetic lock, when realizing the reliable location of locomotive, incubator, the time of the accurate record of control system incubator of being convenient for and the butt joint of isolator, number of times isoparametric, the automatic control of the traceing back of the later stage of being convenient for and cultivation time etc..

It should be noted that, we can set up the corresponding sensor of automatic control etc. according to the actual needs, so as to realize the utility model discloses an automatic control.

In order to facilitate the accurate butt joint of the incubator and the isolator, as shown in fig. 1 and 4, a guide groove 34 is formed in the outer side wall of the isolator, the second electromagnetic lock is arranged in the guide groove, and the mobile vehicle is provided with a guide post 21 at a position corresponding to the guide groove. When the locomotive moves to the outer flange door department that is close to the isolator, the guide post gets into the guide way in, electromagnetic lock locking guide post.

Preferably, as shown in fig. 6, the second electromagnetic lock may adopt a push-pull type electromagnet with a return spring and capable of automatically returning, wherein an iron core of the electromagnet is radially arranged on the side wall of the guide groove, a cylindrical locking head 181 is arranged at the end of the iron core, and a conical locking hole 211 with a large opening and a small opening is arranged at the outer side of the guide column. When the guide post enters the guide groove, the control system enables the second electromagnetic lock to be electrically operated, the iron core extends forwards, and the locking head at the end of the iron core enters the locking hole of the guide post, so that the guide post and the movable vehicle are locked.

It can be understood that the cylindrical locking head and the conical locking hole form line contact, which is beneficial to forming reliable locking between the cylindrical locking head and the conical locking hole and facilitates quick separation between the locking head and the locking hole.

Furthermore, the outer box and the inner box can be set to be cuboid, a plurality of layers of shelves for placing culture dishes are arranged in the inner box, and correspondingly, the butt joint outlet is set to be rectangular, so that the culture dishes placed on each layer of shelves in the inner box can be transmitted through the butt joint outlet.

Certainly, a carbon dioxide gas cylinder communicated with the inner box through a pipeline and a mobile power supply for supplying power to the incubator can be arranged on the mobile vehicle, so that the incubator can maintain constant carbon dioxide content, the mobile power supply is convenient to move on one hand, and on the other hand, the incubator can be always maintained in a power-on state.

In order to effectively prevent the inside of the inner box from being polluted due to the condensation of water vapor, as another preferable scheme, as shown in fig. 7, a water pan 8 may be further disposed on the inner bottom wall of the inner box near the inner box door, and a heat sink 81 is connected to the bottom wall of the water pan, of course, the heat sink is located between the outer box and the inner box, and the heat sink is communicated with the outside air through a heat dissipation pipeline. That is, the temperature of the radiator and the ambient air are substantially the same, and accordingly, the temperature of the water pan is close to the temperature of the radiator. It is known that when the incubator is in operation, the temperature in the inner chamber will generally be higher than ambient temperature, and therefore the drip tray will be the lowest temperature inside the inner chamber, at which time moisture in the air of the inner chamber will preferentially condense to form condensate within the drip tray. That is to say, when the environment temperature is low and the temperature of the inner box is not enough to keep the temperature of the water pan above the dew point temperature, the inner box only condenses at the water pan to form condensed water, thereby thoroughly avoiding the pollution caused by condensation at other parts of the inner box.

In addition, an automatic sterilization system can be arranged between the inner box and the outer box, the automatic sterilization system comprises a vaporized hydrogen peroxide generation module 91 and a vaporized hydrogen peroxide decomposition module 92, the output ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of an output pipeline 94 in parallel through an electric control valve 93, the input ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of an input pipeline 95 in parallel through an electric control valve, the other end of the input pipeline is communicated with the bottom wall of the inner box, the other end of the output pipeline is communicated with the top wall of the inner box, a circulating fan 96 and a filter 97 are arranged on the input pipeline, a filter is arranged on the output pipeline, and a hydrogen peroxide concentration sensor is arranged in the inner box.

Therefore, the vaporized hydrogen peroxide generation module, the output pipeline and the input pipeline form a sterilization loop through the inner box, and the vaporized hydrogen peroxide decomposition module, the output pipeline and the input pipeline form a decomposition loop through the inner box.

When the electric control valve connected with the input end and the output end of the vaporized hydrogen peroxide decomposition module is closed, the vaporized hydrogen peroxide generation module and the circulating fan are started, the circulating fan can send the vaporized hydrogen peroxide generated by the vaporized hydrogen peroxide generation module into the inner box through the output pipeline for disinfection and sterilization, and the filters on the output pipeline and the input pipeline effectively purify the air in the inner box to the A-level environment to maintain the sterility in the box. When the hydrogen peroxide concentration in the inner box detected by the hydrogen peroxide concentration sensor reaches a set value, the vaporized hydrogen peroxide generation module continues to work for a period of time, and the inner box maintains the hydrogen peroxide sterilization concentration, so that the inner box is effectively sterilized and disinfected. When the sterilization is finished, the electric control valves connected with the input end and the output end of the vaporized hydrogen peroxide generation module are closed, meanwhile, the vaporized hydrogen peroxide generation module stops working, and the electric control valves connected with the input end and the output end of the vaporized hydrogen peroxide decomposition module are opened, so that the sterilization loop is closed, the decomposition loop is opened, and then the inner box is decomposed by hydrogen peroxide. When the hydrogen peroxide concentration in the inner box detected by the hydrogen peroxide concentration sensor is zero, the electric control valve connected with the input end and the output end of the vaporized hydrogen peroxide decomposition module is closed, so that the automatic sterilization and disinfection of the inner box are completed.

Claims (9)

1. A butt-joint structure of a carbon dioxide incubator and an isolator is characterized in that an auxiliary electric heating element is arranged in the outer box door, a butt-joint outlet is formed in one side of the inner box corresponding to the outer box door, an inner flange ring protruding outwards is arranged at the edge of the butt-joint outlet, an inner box door is arranged in the inner flange ring, a first inflatable sealing ring is arranged between the inner flange ring and the inner box door, a transmission outlet is formed in one side of the isolator, an outer flange ring protruding outwards is arranged at the edge of the transmission outlet, an outer flange door is arranged in the outer flange ring, a second inflatable sealing ring is arranged between the outer flange ring and the outer flange door, and a third inflatable sealing ring is arranged outside the outer flange door in the outer flange ring, when the inner flange ring enters the outer flange ring, the outer flange ring is in sealing connection with the inner flange ring through a third inflatable sealing ring, a sterilization space is defined by the outer flange ring, the inner flange ring, the outer flange door and the inner box door, and the sterilization space is connected with the sterilization medium generation module and the sterilization medium decomposition module.

2. The docking structure for a carbon dioxide incubator and isolator as claimed in claim 1, wherein one side of the outer door is rotatably coupled to the outer flange ring and one side of the inner door is rotatably coupled to the inner flange ring.

3. The docking structure of a carbon dioxide incubator and isolator as claimed in claim 1, wherein a turbulent fan is provided in the inner chamber.

4. The docking structure of a carbon dioxide incubator and an isolator as claimed in claim 1, further comprising a first electromagnetic lock for locking the incubator and a second electromagnetic lock for locking the cart, wherein a position sensor for sensing the position of the cart is provided outside the isolator.

5. The docking structure for a carbon dioxide incubator and the isolator as claimed in claim 4, wherein the side wall of the isolator has a guiding groove, the second electromagnetic lock is disposed in the guiding groove, the mobile vehicle has a guiding post at a position corresponding to the guiding groove, and when the mobile vehicle moves to a position close to the outer flange door of the isolator, the guiding post enters the guiding groove and the second electromagnetic lock locks the guiding post.

6. The docking structure of a carbon dioxide incubator and isolator as claimed in claim 1, wherein the docking outlet is rectangular, and the inner chamber is provided with a plurality of shelves for placing culture dishes.

7. The docking structure of a carbon dioxide incubator and a carbon dioxide isolator as claimed in claim 1, wherein a water pan is provided on the inner bottom wall of the inner chamber adjacent to the inner chamber door, and a radiator is connected to the bottom wall of the water pan between the outer chamber and the inner chamber, the radiator being in communication with the outside air through a heat radiation pipe.

8. A docking structure of a carbon dioxide incubator and isolator according to claim 1, is characterized in that an automatic sterilization system is arranged between the inner box and the outer box, the automatic sterilization system comprises a vaporized hydrogen peroxide generation module and a vaporized hydrogen peroxide decomposition module, the output ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of an output pipeline through an electric control valve, the input ends of the vaporized hydrogen peroxide generation module and the vaporized hydrogen peroxide decomposition module are respectively connected with one end of an input pipeline through an electric control valve, the other end of the input pipeline is communicated with the bottom wall of the inner box, the other end of the output pipeline is communicated with the top wall of the inner box, the input pipeline is provided with a circulating fan and a filter, the output pipeline is provided with a filter, and the inner box is provided with a hydrogen peroxide concentration sensor.

9. The docking structure of a carbon dioxide incubator and an isolator as claimed in claim 1, wherein the mobile cart is provided with a carbon dioxide cylinder communicated with the inner chamber through a pipeline and a mobile power supply for supplying power to the incubator.

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