CN213652517U - Enzyme reaction device for animal and plant cultivation - Google Patents
Enzyme reaction device for animal and plant cultivation Download PDFInfo
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- CN213652517U CN213652517U CN202021535909.4U CN202021535909U CN213652517U CN 213652517 U CN213652517 U CN 213652517U CN 202021535909 U CN202021535909 U CN 202021535909U CN 213652517 U CN213652517 U CN 213652517U
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- 241001465754 Metazoa Species 0.000 title claims abstract description 32
- 238000006911 enzymatic reaction Methods 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 57
- 238000001816 cooling Methods 0.000 claims abstract description 27
- 238000009434 installation Methods 0.000 claims description 5
- 238000004804 winding Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 21
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 7
- 230000009467 reduction Effects 0.000 abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 abstract description 4
- 239000001301 oxygen Substances 0.000 abstract description 4
- 239000007789 gas Substances 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 238000012258 culturing Methods 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an animal and plant cultivates and uses enzyme reaction unit belongs to enzyme reaction technical field, which comprises a reactor, the motor is installed on the reactor top, the power take off end of motor is connected with the power input end of pivot, reactor top right side can be dismantled and be connected with bayonet temperature sensor, the top bonds there is the cavity in the reactor, the cavity is run through in the pivot, the air inlet of cavity extends to the inside of intake pipe, reactor right side top can be dismantled and be connected with the outlet duct, cavity bottom sliding connection has the gas-supply pipe. Air enters the reactor and is divided into micro bubbles through the air outlet hole on the air delivery pipe and the dividing hole on the air distribution pipe to contact with the reaction liquid, so that sufficient oxygen can be provided for animal and plant cells, and the reaction is sufficient; when the plug-in temperature sensor detects that the temperature in the reactor is higher, the water pump pumps water and conveys the water into the cooling pipe to cool the reactor, and the reduction of the activity of the animal and plant cells caused by overhigh temperature is avoided.
Description
Technical Field
The utility model relates to an enzyme reaction technical field especially relates to an animal and plant cultivates and uses enzyme reaction unit.
Background
The cell culture comprises animal cell culture and plant cell culture, wherein the animal cell culture refers to a method for simulating basic conditions and environments in a normal physiological state in vivo under an in vitro aseptic condition, separating and culturing organism tissue cells or establishing a cell line, and enabling the cells to grow or propagate in an in vitro culture container for a long time; the plant cell culture is a technology of placing callus or other easily-dispersible tissues in a liquid culture medium under an in vitro condition for shake culture to obtain dispersed free suspension cells, and proliferating the cells through subculture to obtain a large number of cell populations. Any enzyme catalyzes a reaction, and at most, until the equilibrium state is reached, the enzyme can affect the equilibrium regulation, but the equilibrium point is not changed, and the active group of the enzyme can catalyze only one specific reaction, so that the enzyme has efficiency specificity and direction specificity.
Patent No. CN 210886062U discloses a biological enzyme culture reaction box, which is characterized in that a first bevel gear, a second bevel gear and a support rod are arranged, biological enzymes are placed in a clamping groove on the outer side of a support frame, the first bevel gear and the second bevel gear are rotated by a rotating rod in the biological enzyme culture reaction process, the first bevel gear drives the rotating rod of the support rod, the reaction state of each group of biological enzymes is observed through a glass window, and the reaction process of the biological enzymes is timely and effectively recorded and adjusted; through setting up shunt tubes and shower nozzle, when the biological enzyme cultivates the reaction and accomplishes, rivers spout into the reaction box from the shower nozzle, because of the inside splash plectrum that is provided with of shower nozzle for the rotatory blowout of rivers carries out the washing at no dead angle to the reaction box inside, and the dirt at effectual washing reaction box turning does benefit to universal use.
The enzymatic reactions of the prior art have the following disadvantages: 1. when animal and plant cells react with the reaction solution, the air in the reaction solution is insufficient and the reaction is incomplete; 2. the reaction process of animal and plant cell and reaction liquid produces heat to raise the temperature of the reaction liquid and lower the activity of the cell.
SUMMERY OF THE UTILITY MODEL
The utility model provides an enzyme reaction device for culturing animals and plants, air enters into a reactor and is divided into micro bubbles through an air outlet hole on a gas transmission pipe and a dividing hole on a gas dividing pipe to be contacted with reaction liquid, thereby providing sufficient oxygen for animal and plant cells and fully reacting; when the plug-in temperature sensor detects that the temperature in the reactor is higher, the water pump pumps water and conveys the water into the cooling pipe to cool the reactor, and the reduction of the activity of the animal and plant cells caused by overhigh temperature is avoided.
The utility model provides a specific technical scheme as follows:
the utility model provides an enzyme reaction device for culturing animals and plants, which comprises a reactor, wherein the top end of the reactor is provided with a motor, the power output end of the motor is connected with the power input end of a rotating shaft, the right side of the top end of the reactor is detachably connected with an inserted temperature sensor, the top end in the reactor is bonded with a cavity, the rotating shaft runs through the cavity, the air inlet of the cavity extends into the air inlet pipe, the top end of the right side of the reactor is detachably connected with an air outlet pipe, the bottom of the cavity is slidably connected with the air inlet pipe, the air inlet pipe wraps the outer surface of the rotating shaft, the bottom of the air inlet pipe is provided with an air outlet hole, the bottom end of the air inlet pipe is in clearance connection with an air distribution pipe, the surface of the air distribution pipe is provided with a dividing hole, the bottom end of the rotating shaft is, the cooling tube top and water tank intercommunication, the air inlet of cooling tube bottom extends to the inside of water pumper, the water pumper is located the right side of water tank.
Optionally, a feed inlet is formed in the left side of the top end of the reactor, and a bottom support is connected to the bottom in the reactor in a sliding mode.
Optionally, the bottom end of the bottom support is detachably connected with electric telescopic rods, and the electric telescopic rods are arranged on two sides of the bottom in the reactor.
Optionally, the bottom of the right side of the reactor is detachably connected with a discharge pipe, and the gap of the gas outlet pipe is connected with a one-way valve.
Optionally, the current input ends of the motor, the plug-in temperature sensor, the electric telescopic rod and the water pump are electrically connected with an external power supply through a wire.
The utility model has the advantages as follows:
the embodiment of the utility model provides an animal and plant cultivates and uses enzyme reaction unit:
1. air enters the reactor and is divided into micro bubbles through the air outlet hole on the air delivery pipe and the dividing hole on the air distribution pipe to contact with the reaction liquid, so that sufficient oxygen can be provided for animal and plant cells, and the reaction is sufficient; the air after disinfection is conveyed into the cavity through the air inlet pipe, the bottom end of the cavity is connected with the air conveying pipe in a sliding mode, the cavity is communicated with the air conveying pipe, the air conveying pipe is wrapped and fixed on the outer surface of the rotating shaft, the surface of the bottom end of the air conveying pipe is connected with the air distributing pipe, the air is conveyed into the air conveying pipe through the cavity continuously, the bottom end of the air conveying pipe is provided with air outlet holes, the air is divided into small bubbles through the air outlet holes after overflowing through the air outlet pipe, the air continuously diffuses outwards through the dividing holes on the air distributing pipe, the aperture of the dividing holes is far smaller than that of the air outlet holes, the bubbles are divided into a plurality of micro bubbles through the dividing holes, the motor is started to drive the rotating shaft to rotate, the rotating shaft drives the air conveying pipe and the air, can provide enough oxygen for the animal and plant cells to work, and the animal and plant cells and the reaction liquid fully react.
2. When the plug-in temperature sensor detects that the temperature in the reactor is high, the water is pumped by the water pump and conveyed into the cooling pipe to cool the reactor, so that the reduction of the activity of animal and plant cells caused by overhigh temperature is avoided; the plug-in temperature sensor inserts the reactor top and lasts to measuring the temperature in the reactor, water pump start-up when measuring the temperature higher extracts the water in the water tank and carries in the cooling tube, the cooling tube winding is connected at the reactor surface, water carries out the heat exchange with the reactor from cooling tube bottom end in the transport process to the top, the temperature reduces after reaction liquid heat is absorbed by water in the reactor, the water pump lasts to carrying water in the cooling tube, the water flows back to the water tank after carrying the top in the cooling tube, accessible cooling tube water lasts absorbs reaction liquid heat in the reactor, avoid the high temperature to cause the reduction of animal and plant cell activity.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of an enzyme reaction apparatus for culturing animals and plants according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a fan blade of an enzyme reaction apparatus for culturing animals and plants according to an embodiment of the present invention;
FIG. 3 is a schematic view of the air-divided structure of an enzyme reaction apparatus for culturing animals and plants according to an embodiment of the present invention
FIG. 4 is a schematic structural view of a cooling apparatus of an enzyme reaction apparatus for culturing animals and plants according to an embodiment of the present invention.
In the figure: 1. a reactor; 2. a feed inlet; 3. a cavity; 4. a motor; 5. an air inlet pipe; 6. an insertion type temperature sensor; 7. an air outlet pipe; 8. a one-way valve; 9. a rotating shaft; 10. a gas delivery pipe; 11. a gas distributing pipe; 12. a fan blade; 13. a bottom support; 14. a discharge pipe; 15. an electric telescopic rod; 16. mounting blocks; 17. an air outlet; 18. dividing the hole; 19. a cooling tube; 20. a water pump; 21. a water tank.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
An enzyme reaction apparatus for culturing animals and plants according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 4.
Referring to fig. 1-4, an enzyme reaction device for animal and plant cultivation provided by the embodiment of the present invention comprises a reactor 1, a motor 4 is installed on the top of the reactor 1, the power output end of the motor 4 is connected with the power input end of a rotating shaft 9, an insertion type temperature sensor 6 is detachably connected to the right side of the top end of the reactor 1, a cavity 3 is bonded to the inner top end of the reactor 1, the rotating shaft 9 penetrates through the cavity 3, the air inlet of the cavity 3 extends into the air inlet pipe 5, the air outlet pipe 7 is detachably connected to the top end of the right side of the reactor 1, the air delivery pipe 10 is slidably connected to the bottom of the cavity 3, the air delivery pipe 10 wraps the outer surface of the rotating shaft 9, an air outlet hole 17 is formed at the bottom of the air delivery pipe 10, an air distribution pipe 11 is connected to the bottom of the air delivery pipe 10 in, the utility model discloses a water pump, including pivot 9, installation piece 16, cooling tube 19, water tank 21, water pump 20, pivot 9 bottom detachably connected with installation piece 16, installation piece 16 surface bonding has fan piece 12, reactor 1 surface winding is connected with cooling tube 19, cooling tube 19 top and water tank 21 intercommunication, the air inlet of cooling tube 19 bottom extends to the inside of water pump 20, water pump 20 is located the right side of water tank 1.
Illustratively, the reactor 1 comprises a cavity 3, a rotating shaft 9, a gas pipe 10, a gas distribution pipe 11, fan blades 12, a bottom support 13 and an electric telescopic rod 15, a motor 4 is started to drive the rotating shaft 9 to rotate, the rotating shaft 9 rotates to drive the gas pipe 10 and the gas distribution pipe 11 connected with the surface of the rotating shaft 9 to rotate, the fan blades 12 are adhered to the surface of a mounting block 16, the mounting block 16 is fixed at the bottom end of the rotating shaft 9, the rotating shaft 9 rotates to drive the fan blades 12 to rotate to stir reaction liquid, the aperture of a gas outlet 17 on the gas pipe 10 is far larger than that of a segmentation hole 18 on the gas distribution pipe 11, air is conveyed into the cavity 3 through a gas inlet pipe 5 after being sterilized, the cavity 3 is communicated with the gas pipe 10, the air entering the cavity 3 is conveyed into the gas pipe 10, the top end of the gas pipe 10 can rotate in the cavity 3, the gas pipe 10 and the cavity 3 are.
Referring to fig. 1, a feed inlet 2 is arranged on the left side of the top end of the reactor 1, and a bottom support 13 is slidably connected to the bottom of the reactor 1.
As an example, the raw material for the enzyme reaction for animal and plant cultivation is fed into the reactor 1 through the feed port 2, the sealing between the base 13 and the reactor 1 is good, and liquid or the like cannot flow into the bottom of the reactor 1 through the base 13.
Referring to fig. 1, electric telescopic rods 15 are detachably connected to two sides of the bottom end of the bottom support 13, and the electric telescopic rods 15 are installed on two sides of the bottom in the reactor 1.
Illustratively, the reactor 1 provides a mounting platform for the electric telescopic rod 15, and the electric telescopic rod 15 extends and contracts to drive the bottom support 13 connected with the top end to move up or down along the inner wall of the reactor 1.
Referring to fig. 1, a discharge pipe 14 is detachably connected to the bottom of the right side of the reactor 1, and a check valve 8 is connected to the gas outlet pipe 7 in a clearance manner.
In the example, after the enzyme reaction in the reactor 1 is finished, the reaction product leaves the reactor 1 through the discharge pipe 14, the air outlet pipe 7 is connected with the one-way valve 8, and air can only leave the reactor 1 through the air outlet pipe 7 in one way.
Referring to fig. 1, the current input terminals of the motor 4, the plug-in temperature sensor 6, the electric telescopic rod 15 and the water pump 20 are electrically connected with an external power supply through wires.
In the example, the external power supply respectively provides power for the motor 4, the plug-in temperature sensor 6, the electric telescopic rod 15 and the water pump 20, and the motor 4, the plug-in temperature sensor 6, the electric telescopic rod 15 and the water pump 20 can work independently when being connected in parallel.
When the device is used, a plug is inserted into a socket, an external power supply supplies power, raw materials such as reaction liquid and the like are thrown into the reactor 1 through the feed inlet 2 at the top end of the reactor 1, a proper amount of sterilized air is conveyed into the cavity 3 through the air inlet pipe 5 in the reaction process, the bottom end of the cavity 3 is connected with the air conveying pipe 10 in a sliding mode, the cavity 3 is communicated with the air conveying pipe 10, the air conveying pipe 10 is wrapped and fixed on the outer surface of the rotating shaft 9, the bottom end surface of the air conveying pipe 10 is connected with the air distribution pipe 11, the air is conveyed into the air conveying pipe 10 through the cavity 3 continuously, the bottom end of the air conveying pipe 10 is provided with the air outlet 17, the air is divided into small bubbles through the air outlet 17 after overflowing through the air outlet 17, the air continuously diffuses outwards through the dividing hole 18 on the air distribution pipe 11, the aperture of the dividing hole 18 is far smaller than that of the air outlet 17, the bubbles are divided into, the model of the motor 4 is Y90L-4, the rotation of the rotating shaft 9 drives the gas pipe 10 and the gas distribution pipe 11 which are connected with the rotating shaft 9 to rotate, the air is evenly mixed into the reaction liquid through the tiny bubbles which are divided by the gas outlet 17 and the dividing hole 18 in the continuous rotation process, simultaneously the fan blades 12 on the mounting block 16 which is connected with the bottom end of the rotating shaft 9 rotate to stir and mix the reaction liquid, the waste gas such as carbon dioxide and the like which is generated in the continuous reaction process is moved out of the reactor 1 through the single-file method 8 on the gas outlet pipe 7, the plug-in temperature sensor 6 is inserted into the top end of the reactor 1 to continuously measure the temperature in the reactor 1, the plug-in temperature sensor 6 is ZG-8029S, when the measured temperature is higher, after the water pump 20 is started (by the impeller which rotates at high speed), the water in the water tank 21 (obtains energy under the action of inertial centrifugal, the rotating water flies away from the impeller under the action of centrifugal force, and after the water in the pump is thrown out, a vacuum area is formed in the center of the impeller. Water in the water tank 21 passes through the pipe network under the effect of atmospheric pressure) to be carried in the cooling tube 19, water pump 20 model is YL-3003, cooling tube 19 winding is connected at 1 surface in the reactor, water carries out the heat exchange with reactor 1 from 19 internal water of cooling tube 19 of 19 bottom up transportation process, the temperature reduces after the reaction fluid heat is absorbed by water in the reactor 1, water pump 20 lasts to carrying water in the cooling tube 19, the water flows back to in the water tank 21 after carrying the top in the cooling tube 19, the accessible cooling tube 19 internal water lasts absorbs the reaction fluid heat in the reactor 1, avoid the high temperature to cause the reduction of animal and plant cell activity, electric telescopic handle 15 (electric telescopic handle 15 internal motor is after gear reduction after the reaction finishes, drive a pair of screw nut. Become linear motion to the rotary motion of motor, utilize motor just reversing to accomplish the push rod action) and shorten after starting, electric telescopic handle 15 model is XTL50, electric telescopic handle 15 shortens to drive the collet 13 that links to each other and moves down along reactor 1 internal surface, electric telescopic handle 15 continuously moves down and is located the discharging pipe below until collet 13, collet 13 moves down and drives the reaction liquid and moves down, collet 13 rigidity back reaction liquid shifts out the reactor through discharging pipe 14, carry out cleaning and disinfection to reactor 1 after the reaction liquid all flows and reserve the next use.
The utility model relates to an enzyme reaction device for animal and plant cultivation, which comprises a reactor 1; 2. a feed inlet; 3. a cavity; 4. a motor; 5. an air inlet pipe; 6. an insertion type temperature sensor; 7. an air outlet pipe; 8. a one-way valve; 9. a rotating shaft; 10. a gas delivery pipe; 11. a gas distributing pipe; 12. a fan blade; 13. a bottom support; 14. a discharge pipe; 15. an electric telescopic rod; 16. mounting blocks; 17. an air outlet; 18. dividing the hole; 19. a cooling tube; 20. a water pump; 21. the tank and the components are all standard parts or parts known to the person skilled in the art, the structure and the principle of which are known to the person skilled in the art by means of technical manuals or by means of routine experimentation.
It is apparent that those skilled in the art can make various changes and modifications to the embodiments of the present invention without departing from the spirit and scope of the embodiments of the present invention. Thus, if such modifications and variations of the embodiments of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (5)
1. The utility model provides an enzyme reaction device for animal and plant cultivation, includes reactor (1), its characterized in that, motor (4) are installed on reactor (1) top, the power take off end of motor (4) is connected with the power input end of pivot (9), reactor (1) top right side can be dismantled and be connected with bayonet temperature sensor (6), the top bonds in reactor (1) has cavity (3), pivot (9) run through cavity (3), the air inlet of cavity (3) extends to the inside of intake pipe (5), reactor (1) right side top can be dismantled and be connected with outlet duct (7), cavity (3) bottom sliding connection has gas-supply pipe (10), gas-supply pipe (10) parcel is at the surface of pivot (9), venthole (17) have been seted up to gas-supply pipe (10) bottom, gas-supply pipe (10) bottom gap connection has gas-distribution pipe (11), the gas-distributing pipe (11) surface has been seted up and has been cut hole (18), pivot (9) bottom detachable connections has installation piece (16), installation piece (16) surface bonding has fan piece (12), reactor (1) surface winding is connected with cooling tube (19), cooling tube (19) top and water tank (21) intercommunication, the air inlet of cooling tube (19) bottom extends to the inside of water pumper (20), water pumper (20) are located the right side of water tank (21).
2. The enzyme reaction device for cultivating animals and plants according to claim 1, wherein the reactor (1) is provided with a feed inlet (2) at the left side of the top end, and a bottom support (13) is slidably connected to the bottom in the reactor (1).
3. The enzyme reaction device for cultivating animals and plants according to claim 2, wherein the bottom of the bottom support (13) is detachably connected with electric telescopic rods (15), and the electric telescopic rods (15) are installed at two sides of the bottom in the reactor (1).
4. The enzyme reaction device for cultivating animals and plants according to claim 1, wherein a discharge pipe (14) is detachably connected to the bottom of the right side of the reactor (1), and a check valve (8) is connected to the gas outlet pipe (7) in a clearance manner.
5. The enzyme reaction device for cultivating animals and plants according to claim 1, wherein the current input terminals of the motor (4), the plug-in temperature sensor (6), the electric telescopic rod (15) and the water pump (20) are electrically connected with an external power supply through wires.
Priority Applications (1)
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CN202021535909.4U CN213652517U (en) | 2020-07-29 | 2020-07-29 | Enzyme reaction device for animal and plant cultivation |
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CN202021535909.4U CN213652517U (en) | 2020-07-29 | 2020-07-29 | Enzyme reaction device for animal and plant cultivation |
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CN213652517U true CN213652517U (en) | 2021-07-09 |
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Effective date of registration: 20220215 Address after: 528000 Room 501, 5th floor, No. 2, Desheng East Road, fengsha village committee, Daliang sub district office, Shunde District, Foshan City, Guangdong Province Patentee after: FOSHAN SHUNDE AURORA BIOTECHNOLOGY CO.,LTD. Address before: 030032 Tongda street, Xiaodian District, Taiyuan City, Shanxi Province Patentee before: Zhao Yuan |
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