CN213357616U

CN213357616U - Biological cell culture equipment

Info

Publication number: CN213357616U
Application number: CN202022068213.1U
Authority: CN
Inventors: 陈超
Original assignee: Guangzhou Haoxin Cleaning Engineering Technology Co ltd
Current assignee: Guangzhou Haoxin Cleaning Engineering Technology Co ltd
Priority date: 2020-09-21
Filing date: 2020-09-21
Publication date: 2021-06-04
Anticipated expiration: 2030-09-21

Abstract

The utility model discloses a biological cell culture equipment, including culture tank body, fixed bolster, sealed apron, feed inlet, discharge gate, transfer line, mount, agitating unit and drive assembly. The utility model relates to a biological cell culture equipment is through the improvement to agitating unit, be convenient for stir the culture solution fully, the solution concentration that makes to pour into the culture tank becomes balanced through the stirring that does not stop to make the speed of cell growth unanimous, the cell of same batch cultivation is in same growth stage, produced waste gas in the cell culture in addition, through the setting of one-way air outlet valve, make gaseous outside one-way flow only, outside gas can not get into, can improve carminative speed, be favorable to going on of cell cultivation process.

Description

Biological cell culture equipment

Technical Field

The utility model relates to a cell culture equipment correlation technique field specifically is a biological cell culture equipment.

Background

The cell culture technology is also called as cell cloning technology, the formal term in biology is cell culture technology, the whole bioengineering technology or one of the biological cloning technology is an essential process, the cell culture is large-scale cell cloning, the cell culture technology can be used for culturing a large amount of cells into simple single cells or cells with few differentiation, a large amount of cells or metabolites thereof are obtained through cell culture, the growth of the cells needs to be carried out in a sealed culture tank, the infection of external bacteria dust is avoided, and oxygen needs to be provided for culture solution in the culture tank in order to provide the oxygen necessary for the growth of the cells;

cell culture equipment among the prior art is in the use of reality, because it is direct to jar internal air feed to lead to storing up a large amount of gases in the culture tank to lead to more through the pipeline, because cell culture's cycle is longer, for preventing to overstock too much bubbles in the culture tank, need incessantly outwards discharge the bubble, but the outside air can get into jar body through the pipeline, causes the pollution to the culture solution, and agitating unit stirs inadequately in the culture tank that has in addition, is unfavorable for the cultivation reproduction of cell.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a biological cell culture equipment aims at the problem of improvement.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a biological cell culture equipment, including cultivateing the jar body, the setting is at cultivation jar body lower extreme fixed bolster all around, the sealed apron of setting in cultivation jar body upper end, the setting is in sealed apron one side and the feed inlet that is linked together with the cultivation jar body, the air outlet valve of setting in cultivation jar body one side upper end, the discharge gate of setting in cultivation jar body one side lower extreme, the setting is at this internal transfer line of cultivation jar, the setting is at the mount of transfer line and cultivation jar body inner chamber bottom junction, evenly set up the agitating unit in the transfer line periphery and be used for driving the drive assembly of transfer line.

Preferably, a connecting bearing is arranged at the joint of the fixing frame and the transmission rod, the connecting bearing is a ball bearing, and the fixing frame is fixedly connected with the culture tank body.

Preferably, the driving assembly comprises a driving motor, a speed reducer and a rigid coupling, an output shaft of the driving motor is connected with an input shaft of the speed reducer through a connecting flange, an output shaft of the speed reducer is connected with a transmission rod, the transmission rod is connected with the speed reducer through the rigid coupling, a connecting bearing is also arranged at the connecting part of the transmission rod and the rigid coupling, and the driving motor is externally connected with an external power supply through a power supply lead.

Preferably, the stirring device comprises a mounting seat uniformly arranged on the periphery of the transmission rod, stirring blades respectively arranged on two sides of the mounting seat, and connectors arranged at the connecting positions of the stirring blades and the mounting seat.

The preferred, the air outlet valve includes the valve body that is linked together with the culture tank body, set up the slip dog in valve body middle part position, the gas outlet that is linked together with the valve body, set up the regulating spring in slip dog one end and set up the valve gap of keeping away from the department of being connected with the culture tank at the valve body, regulating spring's one end and slip dog fixed connection, regulating spring's the other end and the inboard fixed connection of valve gap, and the tangent setting of regulating spring's periphery and valve body inner chamber.

Preferably, the device also comprises a sealing cover arranged on the feeding hole and an adjusting valve arranged on the discharging hole, and a sealing gasket is arranged at the joint of the sealing cover and the feeding hole.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model has the characteristics of reasonable in design and easy operation, the utility model relates to a biological cell culture equipment

(1) The utility model has the advantages that the stirring device is improved, so that the culture solution can be stirred sufficiently, the concentration of the solution poured into the culture tank can be balanced through continuous stirring, the growth speed of the cells is consistent, the cells cultured in the same batch are in the same growth stage, and in addition, the waste gas generated in the cell culture is generated;

(2) through the setting of one-way air outlet valve, make gaseous outside unidirectional flow only, outside gas can not get into, can improve carminative speed, is favorable to going on of cell cultivation process.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural view of a partial cross-sectional view of the overall structure of the biological cell culture apparatus of the present invention;

FIG. 2 is a schematic structural view of the front view of FIG. 1 in the biological cell culture apparatus of the present invention;

FIG. 3 is a schematic structural view of the biological cell culture apparatus of FIG. 1 from another perspective;

FIG. 4 is a schematic structural diagram of an enlarged view of the portion A of FIG. 3 in the biological cell culture apparatus of the present invention.

In the figure: 1-culture tank body, 2-sealing cover plate, 3-transmission rod, 4-fixing frame, 5-fixing support, 6-mounting seat, 7-stirring blade, 71-connecting head, 8-driving motor, 9-speed reducer, 10-feeding hole, 11-discharging hole, 12-valve body, 121-air outlet, 13-sliding stop block, 14-regulating spring and 15-valve cover.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1, fig. 2, fig. 3 and fig. 4, the present invention provides a technical solution: a biological cell culture device comprises a culture tank body 1, a fixed bracket 5 arranged around the lower end of the culture tank body 1, a sealing cover plate 2 arranged at the upper end of the culture tank body 1, a feed inlet 10 arranged at one side of the sealing cover plate 2 and communicated with the culture tank body 1, an air outlet valve arranged at the upper end of one side of the culture tank body 1, a discharge port 11 arranged at the lower end of one side of the culture tank body 1, a transmission rod 3 arranged in the culture tank body 1, a fixed frame 4 arranged at the connection part of the transmission rod 3 and the bottom of the inner cavity of the culture tank body 1, a stirring device uniformly arranged at the periphery of the transmission rod 3 and a driving component for driving the transmission rod 3, the utility model discloses an improvement on the stirring device, which is convenient for fully stirring culture solution, and enables the concentration of the solution poured into the culture tank to be balanced through continuous stirring, thereby enabling the growth, the cells cultured in the same batch are in the same growth stage, and in addition, the waste gas generated in the cell culture can only flow outwards in a single direction through the arrangement of the one-way gas outlet valve, so that the external gas can not enter, the exhaust rate can be improved, and the cell culture process is facilitated.

Referring to fig. 1, 2, 3 and 4, a connecting bearing is arranged at the joint of the fixing frame 4 and the transmission rod 3, the connecting bearing is a ball bearing, the fixing frame 4 is fixedly connected with the culture tank body 1, so that the lower end of the transmission rod 3 is conveniently supported by the fixing frame 4, the rotation of the transmission rod 3 in the rotation process is avoided, and the driving stability of the transmission rod 3 for the stirring device is effectively ensured.

Referring to fig. 1, 2, 3 and 4, the driving assembly includes a driving motor 8, a reducer 9 and a rigid coupling, an output shaft of the driving motor 8 is connected to an input shaft of the reducer 9 through a connecting flange, an output shaft of the reducer 9 is connected to the transmission rod 3, the transmission rod 3 is connected to the reducer 9 through the rigid coupling, a connecting bearing is also disposed at a connection position of the transmission rod 3 and the rigid coupling, the driving motor 8 is externally connected to an external power source through a power lead, so that the rotation frequency of the driving motor 8 is conveniently controlled through the reducer 9, the transmission rod 3 is conveniently driven to rotate through the rigid coupling, and the stirring device is conveniently driven through the transmission rod 3.

Referring to fig. 1, 2, 3 and 4, the stirring device includes a mounting seat 6 uniformly disposed on the periphery of the transmission rod 3, stirring blades 7 respectively disposed on two sides of the mounting seat 6, and a connecting head 71 disposed at a connecting portion between the stirring blades 7 and the mounting seat 6, so that the stirring blades 7 are connected to the mounting seat 6 through the connecting head 71, and the transmission rod 3 is rotated to drive the stirring device to rotate.

Referring to fig. 1, 2, 3 and 4, the gas outlet valve includes a valve body 12 communicated with the culture tank body 1, a sliding block 13 disposed at a middle position of the valve body 12, a gas outlet 121 communicated with the valve body 12, an adjusting spring 14 disposed at one end of the sliding block 13, and a valve cover 15 disposed at the valve body 12 far away from a connection position with the culture tank, one end of the adjusting spring 14 is fixedly connected with the sliding block 13, the other end of the adjusting spring 14 is fixedly connected with an inner side of the valve cover 15, and an outer periphery of the adjusting spring 14 is tangential to an inner cavity of the valve body 12, so that the gas outlet 121 is conveniently sealed by the sliding block 13, and then the opening and closing of the gas outlet 121 is conveniently adjusted by the sliding block 13, thereby facilitating the discharge of waste gas in the.

Referring to fig. 1, 2, 3 and 4, the device further includes a sealing cover disposed on the feeding port 10 and an adjusting valve disposed on the discharging port 11, wherein a sealing gasket is disposed at a connection position of the sealing cover and the feeding port 10, so that the feeding port 10 is conveniently sealed by the sealing cover, and the discharging efficiency of the discharging port 11 is conveniently adjusted by the adjusting valve.

The working principle is as follows: the culture solution is injected into the culture tank body 1 by opening the sealing cover on the feeding hole 10, then the driving motor 8 drives the transmission shaft to rotate by opening the external power supply of the driving motor 8, thereby facilitating the transmission shaft to drive the stirring blades 7 to rotate, further facilitating the stirring blades 7 to continuously stir the culture solution, as the cells generate waste gas continuously in the process of culturing, the concentration of the waste gas is increased continuously, the sliding block 13 in the valve body 12 is pushed by the waste gas to continuously apply pressure to the sliding block 13, since the adjusting spring 14 is pressed by the valve cover 15 and the sliding block 13, the adjusting spring 14 is contracted, so that the sliding block 13 moves away from the air outlet 121 on the valve body 12, thereby being convenient for discharging the waste gas through the gas outlet 121, and the cultured cells are discharged by opening the regulating valve on the discharge hole 11.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological cell culture apparatus, characterized in that, including culture tank body (1), setting culture tank body (1) lower extreme fixed bolster (5) all around, set up sealed apron (2), the setting of culture tank body (1) upper end are in sealed apron (2) one side and with culture tank body (1) feed inlet (10) that are linked together, set up culture tank body (1) one side upper end air outlet valve, set up and be in culture tank body (1) one side lower extreme discharge gate (11), setting are in transfer line (3), the setting in culture tank body (1) are in transfer line (3) and culture tank body (1) inner chamber bottom junction's mount (4), the even setting are in the agitating unit of transfer line (3) periphery and be used for driving the drive assembly of transfer line (3).

2. The biological cell culture equipment according to claim 1, wherein a connecting bearing is arranged at the joint of the fixing frame (4) and the transmission rod (3), the connecting bearing is a ball bearing, and the fixing frame (4) is fixedly connected with the culture tank body (1).

3. The biological cell culture equipment according to claim 2, wherein the driving assembly comprises a driving motor (8), a speed reducer (9) and a rigid coupling, an output shaft of the driving motor (8) is connected with an input shaft of the speed reducer (9) through a connecting flange, an output shaft of the speed reducer (9) is connected with the transmission rod (3), the transmission rod (3) is connected with the speed reducer (9) through the rigid coupling, a connecting bearing is arranged at the connecting part of the transmission rod (3) and the rigid coupling, and the driving motor (8) is externally connected with an external power supply through a power supply lead.

4. A biological cell culture apparatus according to claim 3, wherein the stirring device comprises a mounting seat (6) uniformly arranged on the periphery of the transmission rod (3), stirring blades (7) respectively arranged on two sides of the mounting seat (6), and a connecting head (71) arranged at the connecting part of the stirring blades (7) and the mounting seat (6).

5. The biological cell culture equipment according to claim 4, wherein the gas outlet valve comprises a valve body (12) communicated with the culture tank body (1), a sliding stop block (13) arranged in the middle of the valve body (12), a gas outlet (121) communicated with the valve body (12), an adjusting spring (14) arranged at one end of the sliding stop block (13), and a valve cover (15) arranged at the position, far away from the connection part with the culture tank, of the valve body (12), one end of the adjusting spring (14) is fixedly connected with the sliding stop block (13), the other end of the adjusting spring (14) is fixedly connected with the inner side of the valve cover (15), and the periphery of the adjusting spring (14) is arranged in a tangent mode with the inner cavity of the valve body (12).

6. The biological cell culture equipment according to claim 5, further comprising a sealing cover arranged on the feeding port (10) and an adjusting valve arranged on the discharging port (11), wherein a sealing gasket is arranged at the position where the sealing cover is connected with the feeding port (10).