CN218262553U

CN218262553U - Cell culture equipment for culturing gamma delta t cells

Info

Publication number: CN218262553U
Application number: CN202222201861.9U
Authority: CN
Inventors: 阮志; 李志刚; 黄宇; 唐雯
Original assignee: Beijing Zhongrui United Biotechnology Co ltd
Current assignee: Beijing Zhongrui United Biotechnology Co ltd
Priority date: 2022-08-22
Filing date: 2022-08-22
Publication date: 2023-01-10
Anticipated expiration: 2032-08-22

Abstract

The utility model discloses a cultivate cell culture equipment for gamma delta T cell, the on-line screen storage device comprises a base, the incubator body and sealed lid, sealed lid is supporting to be established on the top of the incubator body, the last fixed surface of base has the fixed plate, the side of fixed plate is rotated through the bearing and is connected with the pivot, the outside fixed connection of pivot and the incubator body, the center of sealed lid bottom is rotated through the bearing and is connected with the cavity pivot, the circumference side evenly distributed that the cavity pivot is located the incubator internal chamber is fixed with stirring vane, this cultivate cell culture equipment for gamma delta T cell, and reasonable and practical, the operation is convenient, can high efficiency carry out synchronous automatic stirring and shake the shaking even to the culture medium in the incubator body, stirring efficiency is high, and is effectual, and through water pump and pivoted cavity pivot, stirring vane, shunt tubes and diffluence hole, can add new culture medium by synchronous even stirring, make its and former culture medium quick even mixture, be favorable to better the amplification culture that carries out gamma delta T cell.

Description

Cell culture equipment for culturing gamma delta t cells

Technical Field

The utility model relates to the field of biotechnology, specifically be a cultivate cell culture equipment for gamma delta t cell.

Background

γ δ T cells are T cells that perform innate immune functions, the TCR of which consists of γ and δ chains. The gamma delta T cell is an immune cell which can kill cancer cells and tumor stem cells and can recognize cancer antigens. In recent years, with the rapid development of techniques such as cell molecular biology and genetic engineering, cellular immunotherapy has become a new mode of the fourth tumor therapy following three conventional therapies, i.e., surgery, chemotherapy and radiotherapy. Researches show that the gamma delta T cells have the characteristics of non-MHC restricted killing of tumor cells, so that the gamma delta T cells can be an ideal cell preparation raw material, can directly start to kill the tumor cells under the action of no antigen presenting cells (APC cells), and also has good killing effect on the tumor cells with the tendency of immune escape and low expression of MHC molecules.

At present, in the process of culturing γ δ T cells, it is necessary to stir the culture medium to promote propagation and growth of T cells, and to add new culture medium many times to perform amplification culture of γ δ T cells, however, the cell culture apparatus for culturing γ δ T cells in the prior art has a complex structure, is troublesome to operate, has a poor stirring effect, is not uniform enough to stir, and is not convenient to mix new culture medium and original culture medium quickly and uniformly because the stirring and adding functions are performed separately, which not only increases the difficulty and workload of cell culture, but also facilitates the rapid and uniform mixing of new culture medium and original culture medium, so as to perform amplification culture of γ δ T cells more quickly and better, and brings great inconvenience to the culture work of γ δ T cells.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome current defect, provide a cultivate cell culture equipment for gamma delta t cell, can high efficiency carry out synchronous automatic stirring and shake to the culture medium in the culture tank body and shake evenly, stir efficiently, effectual, can evenly stir in step and add new culture medium moreover, make its and former culture medium quick even mixture, can effectively solve the problem in the background art.

In order to achieve the above purpose, the utility model provides a following technical scheme: the utility model provides a cultivate cell culture equipment for gamma delta t cell, includes base, the culture tank body and sealed lid, sealed lid is supporting to be established on the top of the culture tank body, the last fixed surface of base has the fixed plate, the side of fixed plate is rotated through the bearing and is connected with the pivot, the outside fixed connection of pivot and the culture tank body, the center of sealed lid bottom is rotated through the bearing and is connected with the cavity pivot, the circumference side evenly distributed that the cavity pivot is located the culture tank body inner chamber is fixed with stirring vane, the top that the cavity pivot extends to sealed lid top is equipped with rotary joint, the upper surface at sealed lid is fixed in the outside of rotary joint stiff end, rotary joint's rotatory end and cavity pivot top intercommunication, sealed lid is fixed with water pump and liquid storage cylinder in proper order, the delivery port of water pump passes through connecting pipe and rotary joint's stiff end intercommunication, the water inlet of water pump passes through the inner chamber intercommunication of connecting pipe and liquid storage cylinder, the circumference side evenly distributed intercommunication of cavity pivot has the shunt tubes, the circumference side evenly distributed of shunt tubes has seted up the reposition of redundant personnel hole.

As a preferred technical scheme of the utility model, the side of fixed plate is equipped with control switch group, control switch group and external power source electric connection, water pump and control switch group electric connection.

As a preferred technical scheme of the utility model, the another side of fixed plate is fixed with the motor, the output shaft of motor and the outside end fixed connection of pivot, motor and control switch group electric connection.

As an optimal technical scheme of the utility model, still include drive assembly, drive assembly contains pinion, driving motor and master gear, the pinion is fixed and is located the circumference side of sealed lid top in the cavity pivot, driving motor fixes the one side at sealed lid upper surface, the center of master gear bottom and the top fixed connection of driving motor output shaft, the master gear corresponds the meshing with the pinion, driving motor and control switch group electric connection.

As an optimized technical proposal of the utility model, the outer side of the culture tank body is fixed with a fixed ring, and the inner side end of the rotating shaft is fixedly connected with the outer side surface of the fixed ring.

As a preferred technical scheme of the utility model, the outside of rotary joint stiff end is fixed with the mount, the bottom mounting of mount is at the upper surface of sealed lid.

As the utility model discloses a preferred technical scheme, the bottom of the cultivation jar body is the infundibulate structure, the bottom intercommunication of the cultivation jar body is fixed with the drain pipe, be equipped with the valve on the drain pipe.

As an optimal technical scheme of the utility model, the four corners of base bottom all is equipped with the universal wheel of taking the brake block.

Compared with the prior art, the beneficial effects of the utility model are that: this cultivate cell culture equipment for gamma delta T cell, it is rational in infrastructure practical, the simple operation, can high efficiency carry out synchronous automatic stirring and shake the shaking even to the culture medium in the culture tank body, it is efficient to stir, and is effectual, moreover, through water pump and pivoted cavity pivot, stirring vane, shunt tubes and reposition of redundant personnel hole, can evenly add new culture medium in step to make its and the quick even mixture of former culture medium, be favorable to the better amplification culture who carries out gamma delta T cell.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic view of a local structure of the present invention.

Fig. 3 is an enlarged schematic view of a portion a of fig. 2 according to the present invention.

In the figure: the device comprises a base 1, a fixing plate 2, a motor 3, a rotating shaft 4, a fixing ring 5, a culture tank body 6, a hollow rotating shaft 7, a driving component 8, a pinion 81, a driving motor 82, a main gear 83, stirring blades 9, a rotary joint 10, a fixing frame 11, a water pump 12, a liquid storage barrel 13, a sealing cover 14, a shunt pipe 15, a shunt hole 16, a liquid outlet pipe 17, a universal wheel 18 and a control switch group 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a cell culture device for culturing gamma delta t cells comprises a base 1, a culture tank body 6 and a sealing cover 14, wherein the sealing cover 14 is arranged at the top end of the culture tank body 6 in a matched mode, a fixing plate 2 is fixed on the upper surface of the base 1, a rotating shaft 4 is rotatably connected to the side surface of the fixing plate 2 through a bearing, a motor 3 is fixed to the other side surface of the fixing plate 2, an output shaft of the motor 3 is fixedly connected with the outer side end of the rotating shaft 4, the rotating shaft 4 is fixedly connected with the outer side of the culture tank body 6, concretely, a fixing ring 5 is fixed to the outer side of the culture tank body 6, the inner side end of the rotating shaft 4 is fixedly connected with the outer side surface of the fixing ring 5, the rotating shaft 4 is driven to rotate through the rotation of the motor 3 (the left and right rotating angles of the motor 3 are smaller than 90 degrees), the culture tank body 6 can be driven to shake left and right, and the culture medium in the culture tank body 6 can be further stirred evenly, the center of the bottom of the sealing cover 14 is rotationally connected with a hollow rotating shaft 7 through a bearing, the hollow rotating shaft 7 is evenly distributed and fixed with stirring blades 9 on the circumferential side surface of the inner cavity of the culture tank body 6, the culture tank further comprises a driving component 8, the driving component 8 comprises a secondary gear 81, a driving motor 82 and a main gear 83, the secondary gear 81 is fixed on the circumferential side surface of the hollow rotating shaft 7 above the sealing cover 14, the driving motor 82 is fixed on one side of the upper surface of the sealing cover 14, the center of the bottom of the main gear 83 is fixedly connected with the top end of an output shaft of the driving motor 82, the main gear 83 is correspondingly meshed with the secondary gear 81, the main gear 83 and the secondary gear 81 are driven to rotate through the rotation of the driving motor 82, further the hollow rotating shaft 7 and the stirring blades 9 can be driven to rotate, the culture medium in the culture tank body 6 can be automatically stirred, the top end of the hollow rotating shaft 7 extending to the upper part of the sealing cover 14 is provided with a rotary joint 10, the outer side of the fixed end of the rotary joint 10 is fixed on the upper surface of a sealing cover 14, specifically, a fixing frame 11 is fixed on the outer side of the fixed end of the rotary joint 10, the bottom end of the fixing frame 11 is fixed on the upper surface of the sealing cover 14, the rotary end of the rotary joint 10 is communicated with the top end of a hollow rotating shaft 7, a water pump 12 and a liquid storage barrel 13 are sequentially fixed on the sealing cover 14, a water outlet of the water pump 12 is communicated with the fixed end of the rotary joint 10 through a connecting pipe, a water inlet of the water pump 12 is communicated with an inner cavity of the liquid storage barrel 13 through a connecting pipe, a shunt pipe 15 is communicated with the circumferential side surface of the hollow rotating shaft 7 in an average distribution manner, shunt holes 16 are formed in the circumferential side surface of the shunt pipe 15 in an average distribution manner, when a new culture medium needs to be added, the water pump 12 is started to work, the new culture medium stored in the liquid storage barrel 13 can be sequentially and uniformly added into a culture tank body 6 through the hollow rotating shaft 7, the shunt pipe 15 and the shunt pipe 16, the stirring blades 9 are driven by the hollow rotating of the hollow rotating pipe 7, the shunt pipe 15 and the shunt pipe 16, a power switch group 19 is driven by the hollow rotating of the tank 2, the tank body, the power switch group 19 is connected with a universal switch group control motor 17, the bottom of the culture tank 6, the bottom of the universal switch 17, the culture tank is connected with a liquid pump, the bottom of a universal control motor, the bottom of a liquid outlet pipe 17, the culture tank, the bottom of a universal control motor, the universal control motor 17, the bottom of a universal switch 17, the culture tank is convenient electric control motor, the bottom of a movable control device, the bottom of a movable control motor 17, the bottom of a movable control device, the bottom of a movable type culture tank, the bottom of a movable control motor 17, the bottom of a movable type culture tank.

When in use: the power is switched on, the rotation of the driving motor 82 is controlled by the control switch group 19, the main gear 83 and the pinion 81 are driven to rotate, the hollow rotating shaft 7 and the stirring blades 9 can be driven to rotate, the culture medium in the culture tank body 6 can be automatically stirred, meanwhile, the rotation of the motor 3 is controlled by the control switch group 19 (the left and right rotation angles of the motor 3 are smaller than 90 degrees), the rotating shaft 4 is driven to rotate, the culture tank body 6 can be driven to shake left and right, the culture medium in the culture tank body 6 can be further uniformly stirred, when a new culture medium needs to be added, the water pump 12 is started by the control switch group 19 to work, the new culture medium stored in the liquid storage cylinder 13 can be uniformly added into the culture tank body 6 sequentially through the hollow rotating shaft 7, the shunt pipe 15 and the shunt pipe 16, the hollow rotating shaft 7 drives the stirring blades 9, the shunt pipe 15 and the shunt pipe 16 to rotate, the new culture medium and the original culture medium in the culture tank body 6 can be rapidly and uniformly mixed, and the gamma T cells can be better amplified and cultured.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cultivate cell culture equipment for gamma delta t cell, includes base (1), culture tank body (6) and sealed lid (14), sealed lid (14) are supporting to be established on the top of culture tank body (6), its characterized in that: the last fixed surface of base (1) has fixed plate (2), the side of fixed plate (2) is rotated through the bearing and is connected with pivot (4), the outside fixed connection of pivot (4) and the culture tank body (6), the center of sealed lid (14) bottom is rotated through the bearing and is connected with cavity pivot (7), circumference side evenly distributed that cavity pivot (7) are located the culture tank body (6) inner chamber is fixed with stirring vane (9), the top that cavity pivot (7) extend to sealed lid (14) top is equipped with rotary joint (10), the upper surface at sealed lid (14) is fixed in the outside of rotary joint (10) stiff end, the rotatory end and the cavity pivot (7) top intercommunication of rotary joint (10), sealed lid (14) are fixed with water pump (12) and stock solution section of thick bamboo (13) in proper order, the delivery port of water pump (12) passes through the connecting pipe and the stiff end intercommunication of rotary joint (10), the water inlet of water pump (12) passes through the inner chamber intercommunication of connecting pipe and stock solution section of thick bamboo (13), the circumference evenly distributed intercommunication of pivot (7) has shunt tubes (15), the average shunt tubes (16) of branch flow distribution have been seted up to the side.

2. The cell culture apparatus for culturing γ δ t cells according to claim 1, characterized in that: the side of fixed plate (2) is equipped with control switch group (19), control switch group (19) and external power source electric connection, water pump (12) and control switch group (19) electric connection.

3. The cell culture apparatus for culturing γ δ t cells according to claim 2, characterized in that: the motor (3) is fixed on the other side face of the fixing plate (2), the output shaft of the motor (3) is fixedly connected with the outer side end of the rotating shaft (4), and the motor (3) is electrically connected with the control switch group (19).

4. The cell culture apparatus for culturing γ δ t cells according to claim 3, wherein: still include drive assembly (8), drive assembly (8) contain pinion (81), driving motor (82) and master gear (83), pinion (81) are fixed and are located the circumference side of sealed lid (14) top in cavity pivot (7), one side at sealed lid (14) upper surface is fixed in driving motor (82), the center of master gear (83) bottom and the top fixed connection of driving motor (82) output shaft, master gear (83) correspond the meshing with pinion (81), driving motor (82) and control switch group (19) electric connection.

5. The cell culture apparatus for culturing γ δ t cells according to claim 1, wherein: the outside of the culture tank body (6) is fixed with a fixing ring (5), and the inner side end of the rotating shaft (4) is fixedly connected with the outer side surface of the fixing ring (5).

6. The cell culture apparatus for culturing γ δ t cells according to claim 1, wherein: the outer side of the fixed end of the rotary joint (10) is fixed with a fixing frame (11), and the bottom end of the fixing frame (11) is fixed on the upper surface of a sealing cover (14).

7. The cell culture apparatus for culturing γ δ t cells according to claim 1, wherein: the bottom of the culture tank body (6) is of a funnel-shaped structure, a liquid outlet pipe (17) is fixedly communicated with the bottom of the culture tank body (6), and a valve is arranged on the liquid outlet pipe (17).

8. The cell culture apparatus for culturing γ δ t cells according to claim 1, wherein: four corners of the bottom of the base (1) are provided with universal wheels (18) with brake pads.