CN213624073U - Constant temperature shaking table capable of controlling gas environment of culture room - Google Patents
Constant temperature shaking table capable of controlling gas environment of culture room Download PDFInfo
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- CN213624073U CN213624073U CN202021389995.2U CN202021389995U CN213624073U CN 213624073 U CN213624073 U CN 213624073U CN 202021389995 U CN202021389995 U CN 202021389995U CN 213624073 U CN213624073 U CN 213624073U
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Abstract
The utility model discloses a constant temperature shaking table capable of controlling the gas environment of a shaking table culture chamber, which belongs to the technical field of constant temperature shaking tables, and comprises a shaking table culture chamber which is a sealed cavity and a gas conveying device, wherein the air outlet of the gas conveying device is communicated with the inside of the shaking table culture chamber, and the air inlet of the gas conveying device is respectively connected with a plurality of different gas supply devices; the shake culture room is pressed valve and check valve and outside intercommunication through the accuse of establishing ties, make the shake culture room be in sealed environment, just so can adjust according to the growing environment of the adaptation of culture, can supply with the gas of different kinds for the shake culture room through gaseous feeding device, make this device can be when cultivateing the anaerobe or liking the culture of carbon dioxide, also can use the vibrations function of constant temperature shaking table, make the culture medium can with the culture intensive mixing, the bacterium can not the caking, the culture environment more does benefit to the bacterial growth.
Description
Technical Field
The utility model relates to a constant temperature shaking table technical field especially relates to a can control and cultivate room gaseous environment's constant temperature shaking table.
Background
The constant temperature shaking table has a stainless steel universal fixture, digital display temperature control, stepless speed regulation and good thermal cycle function, is a multipurpose biochemical instrument, and is indispensable laboratory equipment for precision culture and preparation in plant, organism, microorganism, heredity, virus, environmental protection, medical science and other scientific research, education and production departments;
the constant temperature shaking table has more types, and is divided into a water bath, a gas bath and an oil bath according to a combined part:
a gas bath constant temperature shaking table which is a biochemical instrument combining a constant temperature gas box with controllable temperature and an oscillator;
the water bath constant temperature shaking table is a biochemical instrument combining a water bath with controllable temperature and an oscillator;
the oil bath constant temperature shaking table is a biochemical instrument combining an oil bath groove with controllable temperature and an oscillator;
wherein the temperature of the gas bath shaking table is controlled to be 70 ℃ at most, the temperature of the gas bath shaking table is commonly 37 ℃, and the temperature of the water bath shaking table is controlled to be 100 ℃ at most;
the method is characterized in that a constant-temperature shaking table is selected according to the requirements of species cultured in experiments, the species cultured in a laboratory has requirements on oxygen content and pressure conditions besides temperature, at present, when some anaerobic bacteria are cultured, in order to avoid the bacteria from contacting with oxygen, the vibration function of the constant-temperature shaking table is closed, static culture is carried out, the anaerobic bacteria are prevented from contacting with air in the constant-temperature shaking table, but in fact, a culture solution is exposed in an aerobic environment, the anaerobic bacteria are cultured in the environment, inevitably influences the culture of the anaerobic bacteria, and the vibration function of the shaking table is lost in the static culture, so that the bacteria in the culture solution can be agglomerated, and the full mixing of the culture solution and the anaerobic bacteria is not facilitated; there are also some microorganisms or viruses that have certain requirements on environmental stress; there are also cultures which require exposure to the external atmosphere, which may not require nitrogen, or for some plants which, depending on the experimental requirements, require a simulated high proportion of carbon dioxide gas for cultivation, so that a controlled exposure is now required.
Based on this, the utility model designs a can control and cultivate room gaseous environment's constant temperature shaking table to solve above-mentioned problem.
Disclosure of Invention
An object of the utility model is to provide a can control and cultivate room gas environment's constant temperature shaking table, can be different according to the species of cultivation, adjust the gas environment in the shaking table cultivates the room, and nitrogen gas, pure oxygen, air and carbon dioxide can be controlled to this device in the concentration of cultivateing the room to provide better cultivation environment, and can press the cultivation in the area, provide higher pressure environment for the species in the cultivateing room, with the demand that adapts to different species.
The utility model discloses a realize like this: a constant-temperature shaking table capable of controlling the gas environment of a culture chamber comprises a shaking table culture chamber of the constant-temperature shaking table, wherein the shaking table culture chamber is a sealed cavity body, and a sealing cover capable of being opened or closed is arranged on the shaking table culture chamber;
the air outlet of the gas conveying device is communicated with the interior of the shaking table culture chamber, and the air inlet of the gas conveying device is respectively connected with a plurality of different gas supply devices;
shaking table culture room is through the accuse pressure valve and the check valve and outside intercommunication of establishing ties, the exit end of check valve is outside towards shaking table culture room.
Further, the gas supply device comprises a high-efficiency filter, an oxygen cylinder, a nitrogen cylinder and a carbon dioxide cylinder;
an air valve is arranged at an air outlet of the high-efficiency filter, and an air inlet of the high-efficiency filter is arranged outside the shaking table culture chamber; an oxygen valve is arranged at the gas outlet of the oxygen cylinder, a nitrogen valve is arranged at the gas outlet of the nitrogen cylinder, and a carbon dioxide gas valve is arranged at the gas outlet of the carbon dioxide cylinder.
Further, sealed lid passes through screw thread locking with shaking table cultivation room, set up the sealing washer between shaking table cultivation room and the sealed lid, sealed covering has still set up the observation window of making by explosion-proof glass.
Furthermore, a static pressure box is connected between the air outlet of the gas conveying device and the shaking table culture chamber.
Furthermore, an electromagnetic valve is further installed at the air inlet of the gas conveying device, and the electromagnetic valve and the gas conveying device are connected with an external power supply through a timing switch.
Further, the pressure control valve is an electric pressure reducing valve.
Furthermore, the box body of the shaking table culture chamber is a pressure-resistant box body.
Further, the gas delivery device is an air compressor.
The utility model has the advantages that: the utility model can put the culture into the shaking table culture chamber by controlling the sealing cover, and the shaking table culture chamber is in a sealed environment, thus the device can be adjusted according to the growth environment of the adaptation of the culture, different kinds of gas can be supplied to the shaking table culture chamber by the gas supply device, the device can also use the vibration function of the constant temperature shaking table when culturing the anaerobe or the culture liking carbon dioxide, the culture medium can be fully mixed with the culture, the environment more beneficial to culture can be provided, and the device is also provided with the pressure control valve and the check valve which are connected in series, the pressure control valve can pump gas into the shaking table culture chamber by the gas conveying device when being closed, thus the air pressure in the shaking table culture chamber is increased, the environment with pressure is created for the culture, such as bacteria living in blood vessels, and the blood pressure of the blood vessels is achieved, the device can simulate the pressure in a blood vessel, the amounts of oxygen and carbon dioxide in veins and arteries are different, the device can also simulate different oxygen-containing environments, the difference of simulated growth environments is achieved through the mixing of gas and culture media, the device can balance the air pressure in the shaking table culture chamber with the outside through the outflow of a check valve by opening a pressure control valve, a normal pressure environment is created, the check valve can prevent polluted air outside the shaking table culture chamber from entering the shaking table culture chamber, the culture cannot be polluted, the experimental result is not influenced, the device can provide more gas environments and culture environments for the culture, so that when the anaerobic bacteria are cultured, the culture media of the anaerobic bacteria can be mixed with nitrogen or carbon dioxide in the shaking table culture chamber through the vibration function of the shaking table, and the anaerobic bacteria and the culture media are uniformly mixed through vibration, the bacteria can not agglomerate, and the culture environment is more beneficial to the growth of the bacteria.
Drawings
The invention will be further described with reference to the following examples with reference to the accompanying drawings.
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a top view of the shaking culture chamber of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1-a shaking table culture chamber, 11-a sealing cover, 12-a rotating handle, 2-a high-efficiency filter, 21-an air valve, 3-a pressure control valve, 31-a check valve, 4-a static pressure box, 5-a gas conveying device, 51-an electromagnetic valve, 52-a timing switch, a 6-oxygen cylinder, a 61-oxygen valve, a 7-nitrogen cylinder, a 71-nitrogen valve, an 8-carbon dioxide cylinder and a 81-carbon dioxide valve.
Detailed Description
Referring to fig. 1 to 2, the present invention provides a technical solution: a constant-temperature shaking table capable of controlling the gas environment of a culture chamber comprises a shaking table culture chamber 1 of the constant-temperature shaking table, wherein the shaking table culture chamber 1 is a sealed cavity, and a sealing cover 11 capable of being opened or closed is arranged on the shaking table culture chamber 1;
the air outlet of the gas conveying device 5 is communicated with the interior of the shaking table culture chamber 1, and the air inlet of the gas conveying device 5 is respectively connected with a plurality of different gas supply devices;
the shaking table culture chamber 1 is communicated with the outside through the pressure control valve 3 and the check valve 31 which are connected in series, the outlet end of the check valve 31 faces the outside of the shaking table culture chamber 1, the culture can be placed into the shaking table culture chamber 1 through the control sealing cover 11, and the shaking table culture chamber 1 is in a sealed environment, so that the shaking table culture chamber can be adjusted according to the adapted growth environment of the culture, different gases such as nitrogen, oxygen, air, carbon dioxide and the like can be provided for the shaking table culture chamber 1 through the gas supply device, the device can also use the vibration function of a constant-temperature shaking table when culturing anaerobic bacteria or liking the culture of carbon dioxide, a culture medium can be fully mixed with the culture, the environment which is more beneficial to culture can be provided, the pressure control valve 3 and the check valve 31 which are connected in series are also arranged on the device, the pressure control valve 3 can pump gas into the shaking table culture chamber 1 through the, thus, the air pressure in the shaking table culture chamber 1 is increased, an environment with pressure is created for the culture, for example, bacteria living in blood vessels, the blood vessels have blood pressure, the device can simulate the pressure in the blood vessels, the amount of oxygen and carbon dioxide in veins and arteries is different, the device can also simulate different oxygen-containing environments, the different simulated growth environments are achieved by mixing gas and culture media, the device can achieve the purpose of simulating the difference of the growth environments by opening the pressure control valve 3, the air pressure in the shaking table culture chamber 1 can flow out through the check valve 31 to be balanced with the outside, a normal pressure environment is created, the check valve 31 can prevent polluted air outside the shaking table culture chamber 1 from entering the shaking table culture chamber 1, the culture cannot be polluted, the experimental result is not influenced, the device can provide more gas environments and culture environments for the culture, thus, when anaerobic bacteria are cultured, also can make the culture medium of anaerobe can mix with nitrogen gas or carbon dioxide in the shake culture room through the vibrations function of shaking table to make anaerobe and culture medium misce bene through vibrations, the bacterium can not clump or agglomerate, cultivates the environment and more does benefit to the bacterial growth.
Wherein, the gas supply device comprises a high-efficiency filter 2, an oxygen cylinder 6, a nitrogen cylinder 7 and a carbon dioxide cylinder 8;
an air valve 21 is arranged at an air outlet of the high-efficiency filter 2, and an air inlet of the high-efficiency filter 2 is arranged outside the shaking table culture chamber 1; the gas outlet of the oxygen cylinder 6 is provided with an oxygen valve 61, the gas outlet of the nitrogen cylinder 7 is provided with a nitrogen valve 71, the gas outlet of the carbon dioxide cylinder 8 is provided with a carbon dioxide gas valve 81, the arrangement can control other supply independently and can also mix the gas proportion in the shaking table culture chamber 1, the operation is convenient, the sealing cover 11 is locked with the shaking table culture chamber 1 through screw threads, a sealing ring is arranged between the shaking table culture chamber 1 and the sealing cover 11, the sealing cover 11 is also provided with an observation window made of explosion-proof glass to ensure the air tightness in the shaking table culture chamber 1 and conveniently observe the internal environment and the culture state, the shaking table culture chamber 1 is isolated from the outside after the sealing cover 11 is tightly covered, and a static pressure box 4 is also connected between the air outlet of the gas conveying device 5 and the shaking table culture chamber 1 to ensure the stable input of charging air flow and low air speed without influencing the charging, and can be with the stable discharge of the gaseous original in the shake culture room 1, solenoid valve 51 has still been installed to gas delivery mechanism 5's air intake, solenoid valve 51 and gas delivery mechanism 5 all are connected with external power supply through time switch 52, reach the effect of opening regularly or closing like this, can last make-up gas in the unmanned on duty condition, pressure control valve 3 is electronic relief pressure valve, do not need the manual operation, the automatic gaseous pressure that keeps in shake culture room 1, the box of shake culture room 1 is the pressure-resistant box, make this device can the area environment of pressing, gas delivery mechanism 5 is air compressor, can aerify in the shake culture room 1, make the interior area of shake culture room 1 press.
One specific application of the embodiment is as follows: the utility model discloses a four-way pipe and four high-pressure hoses, one by one high efficiency filter 2, oxygen cylinder 6, nitrogen cylinder 7 and carbon dioxide gas cylinder 8 all with unobstructed the being connected of air inlet of gas delivery device 5, and need to ensure that the junction is kept apart sealedly, and whether each gas cylinder all has corresponding pneumatic valve control to open or not, oxygen cylinder 6's exit is supplied with through oxygen valve 61 control oxygen and is opened or close, nitrogen cylinder 7's exit is supplied with through nitrogen valve 71 control nitrogen and is opened or close, carbon dioxide gas's supply is opened or is closed through carbon dioxide pneumatic valve 81 control carbon dioxide gas in carbon dioxide gas cylinder 8's exit, and air valve 21 can control whether the pipeline between high efficiency filter 2 and the gas delivery device 5 is unobstructed, open air valve 21 then can the air intake, close then can not the air intake, and the air all is purified through high efficiency filter 2, the standard sterile environment requirement of a laboratory can be met, air can be continuously supplied to the internal circulation of the shaking table culture room 1, and the experimental result of the laboratory cannot be influenced, so that the high-efficiency filter 2 sucks standard sterile pollution-free air in a certain sense;
when the device is used, in example 1, a laboratory needs to cultivate a fungus with anaerobic favorite carbon dioxide in a micro-pressure environment, at this time, the fungus needs to be put into the shaking table cultivation chamber 1 first, then the handle 12 is rotated by the knob, the sealing cover 11 is screwed off and the shaking table cultivation chamber 1 of the constant temperature shaking table is ensured to be in a sealing state, the shaking table cultivation chamber 1 is a high pressure resistant sealing box body, the air valve 21, the oxygen valve 61 and the nitrogen valve 71 are closed, the carbon dioxide air valve 81 is ensured to be in an opening state, then the electromagnetic valve 51 is opened to be in a smooth state, at this time, the carbon dioxide in the carbon dioxide gas bottle 8 is compressed and pressurized into the static pressure box 4 through the gas conveying device 5, so that the shaking table cultivation chamber 11 is filled with air smoothly and slowly, the area of the air outlet of the static pressure box 4 exceeds half of the, thus, it can be ensured that the air flow flowing into the shake cultivation chamber 1 is more uniform, at this time, the pressure control valve 3 is opened, the original air in the shake cultivation chamber 1 is pushed out from the outlet of the check valve 31 by the carbon dioxide gas, because the carbon dioxide gas slowly flows from the static pressure box 4 on the right side to the left side, the original air in the shake cultivation chamber 1 is continuously pushed to the left and is discharged from the outlet of the check valve 31, then after exhausting for a period of time, the original air in the shake cultivation chamber 1 is ensured to be exhausted, even if the air is not completely exhausted, the concentration of the carbon dioxide gas is very high, then the pressure control valve 3 is closed, at this time, the gas conveying device 5 still continues to pump the carbon dioxide gas into the shake cultivation chamber 1, so that the shake cultivation chamber 1 has a certain pressure, and an observation window is arranged on a common constant temperature shaking bed, the observation window of the device can be arranged on the side wall box body, the device is arranged on the sealing cover 11 and is also provided with the observation window made of explosion-proof glass, a pressure gauge can be arranged in the shaking table culture chamber 1, according to the pressure measurement condition, the pressure control valve 3 can be opened or closed, so that the shaking table culture chamber 1 can be decompressed or pressurized, the pressure control valve 3 is opened, carbon dioxide with larger pressure in the shaking table culture chamber 1 can flow through the check valve 31 from the pressure control valve 3, so that the pressure of the shaking table culture chamber 1 is reduced, otherwise, the pressure control valve 3 is closed, and the gas conveying device 5 pumps the carbon dioxide gas into the shaking table culture chamber 1 from the carbon dioxide gas bottle 8, so that the pressure in the shaking table culture chamber 1; at this time, the temperature control function of the constant temperature shaking table is started to adjust the holding temperature of the shaking table, and the vibration function can be started to mix the culture medium of anaerobic fungi with the carbon dioxide in the shaking table culture chamber 1, and there is no fear that oxygen or nitrogen is mixed with the culture medium, such an environment is more suitable for the culture of anaerobic fungi which prefers carbon dioxide in this embodiment, because the culture generally needs a continuous time, in this embodiment, when the fungi are cultured, the carbon dioxide gas valve 81 of the device needs to be continuously opened, the electromagnetic valve 51 is in a normally closed state, the interval time for opening the timing switch 52 is set during the culture, the electromagnetic valve 51 is controlled to be opened and the gas delivery device 5 is started at regular time through the timing switch 52, so that gas is continuously supplemented into the shaking table culture chamber 1, the pressure control valve 3 is an electric pressure reducing valve, after the pressure value can be set, when the pressure in, the pressure control valve 3 is opened to release pressure; when the pressure in the shaking table culture chamber 1 is lower than a set pressure value, the pressure control valve 3 is closed, so that gas in the shaking table culture chamber 1 does not flow out, the pressure is continuously increased, and the pressure control valve 3 can also be manually controlled when the gas which is not needed is initially removed;
when the device is used for culturing a culture in a normal-temperature normal-pressure environment, the carbon dioxide gas valve 81, the oxygen valve 61 and the nitrogen valve 71 are all closed, so that the air valve 21 and the pressure control valve 3 are in an open state, then the gas conveying device 5 can continuously suck other outside air through the high-efficiency filter 2 under the action of the timing switch, and new air is supplemented to enter the shaking table culture chamber 1, so that air is continuously supplemented to enter the shaking table culture chamber 1 when the constant-temperature shaking table is opened, pressure change cannot occur, air in the shaking table culture chamber 1 can flow out from the pressure control valve 3 through the check valve 31, the check valve 31 can prevent polluted air outside the shaking table culture chamber 1 from entering the shaking table culture chamber, the cleanness of the environment in the shaking table culture chamber 1 is kept and not influenced by the outside, and the heat preservation function of vibration of the constant-; similarly, the nitrogen cylinder 7 and the oxygen cylinder 6 are used in the environment with requirements for oxygen and nitrogen, such as aerobic bacteria or aerobic microorganisms, and for example, when no bacterium is wanted to act on any gas, inert nitrogen is used, nitrogen is also a common gas in biological experiments and is used in a way different from carbon dioxide, and the device can control the gas supply time of the nitrogen cylinder 7, the oxygen cylinder 6, the carbon dioxide cylinder 8 and the high efficiency filter 2 to adjust the proportion of the mixed gas in the shaking table culture chamber 1, so that the shaking table culture chamber 1 can be filled with the mixed gas with high purity;
the device not only can allocate proper gas to fill the whole shaking table culture chamber 1 according to the requirement of the culture, so that the constant temperature shaking table can react with the proper gas according to the requirement when in culture, or does not react with the gas at all, and no matter what kind of culture is cultured, the vibration function of the constant temperature shaking table cannot be used due to the reason of the gas in the shaking table culture chamber 1, the device not only can match the culture gas required by the experiment, but also can achieve the purpose of fully reacting the culture medium and the culture when the constant temperature shaking table vibrates, and the vibration condition of anaerobic bacteria in the air or the vibration condition of the constant temperature shaking table cannot be generated, the device not only can lead the culture effect to be better by allocating the gas, but also can not start the vibration mixing function of the constant temperature shaking table due to the reason of gas mismatch, so that the mixing effect is better, the culture effect is better for the living environment of culture reaches the experimental demand, for example the extreme living environment of bacterium, or the best living environment of bacterium, vibrations make anaerobe and culture medium misce bene, and the bacterium can not the conglomeration.
In addition, the box body of the shaking culture chamber 1 is a pressure-resistant box body, such as a stainless steel box body.
Although specific embodiments of the present invention have been described, it will be understood by those skilled in the art that the specific embodiments described are illustrative only and are not limiting upon the scope of the invention, and that equivalent modifications and variations can be made by those skilled in the art without departing from the spirit of the invention, which is to be limited only by the claims appended hereto.
Claims (6)
1. The utility model provides a can control incubator gas environment's constant temperature shaking table which characterized in that: the shaking table culture chamber (1) comprises a constant-temperature shaking table, the shaking table culture chamber (1) is a sealed cavity, and a sealing cover (11) capable of being opened or closed is arranged on the shaking table culture chamber (1);
the air outlet of the gas conveying device (5) is communicated with the interior of the shaking table culture chamber (1), and the air inlet of the gas conveying device (5) is respectively connected with a plurality of different gas supply devices;
shaking table cultivation room (1) is through pressure control valve (3) and check valve (31) and the outside intercommunication of establishing ties, the exit end of check valve (31) is outside towards shaking table cultivation room (1).
2. The apparatus of claim 1, wherein the apparatus further comprises a gas source for supplying gas to the chamber, the gas source comprising: the gas supply device comprises a high-efficiency filter (2), an oxygen cylinder (6), a nitrogen cylinder (7) and a carbon dioxide cylinder (8);
an air valve (21) is arranged at an air outlet of the high-efficiency filter (2), and an air inlet of the high-efficiency filter (2) is arranged outside the shaking table culture chamber (1); the gas outlet of oxygen cylinder (6) has set up oxygen valve (61), the gas outlet of nitrogen cylinder (7) has set up nitrogen gas valve (71), the gas outlet of carbon dioxide gas cylinder (8) has set up carbon dioxide gas valve (81).
3. The apparatus of claim 1, wherein the apparatus further comprises a gas source for supplying gas to the chamber, the gas source comprising: sealed lid (11) and shake culture room (1) pass through screw thread locking, shake culture room (1) and sealed lid (11) between set up the sealing washer, sealed lid (11) are gone up and still have been set up the observation window of making by explosion-proof glass.
4. The apparatus of claim 1, wherein the apparatus further comprises a gas source for supplying gas to the chamber, the gas source comprising: and a static pressure box (4) is also connected between the air outlet of the gas conveying device (5) and the shaking table culture chamber (1).
5. The apparatus of claim 1, wherein the apparatus further comprises a gas source for supplying gas to the chamber, the gas source comprising: the air inlet of the gas conveying device (5) is also provided with an electromagnetic valve (51), and the electromagnetic valve (51) and the gas conveying device (5) are connected with an external power supply through a timing switch (52).
6. The apparatus of claim 1, wherein the apparatus further comprises a gas source for supplying gas to the chamber, the gas source comprising: the pressure control valve (3) is an electric pressure reducing valve; the gas delivery device (5) is an air compressor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021389995.2U CN213624073U (en) | 2020-07-15 | 2020-07-15 | Constant temperature shaking table capable of controlling gas environment of culture room |
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| CN202021389995.2U CN213624073U (en) | 2020-07-15 | 2020-07-15 | Constant temperature shaking table capable of controlling gas environment of culture room |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114214197A (en) * | 2021-12-30 | 2022-03-22 | 苏州培英实验设备有限公司 | Carbon dioxide protection's constant temperature and humidity shaking table |
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2020
- 2020-07-15 CN CN202021389995.2U patent/CN213624073U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114214197A (en) * | 2021-12-30 | 2022-03-22 | 苏州培英实验设备有限公司 | Carbon dioxide protection's constant temperature and humidity shaking table |
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