CN213537888U

CN213537888U - Multifunctional bioreactor

Info

Publication number: CN213537888U
Application number: CN202022338263.7U
Authority: CN
Inventors: 张燕
Original assignee: Suzhou Qianyun Instrument Technology Co ltd
Current assignee: Suzhou Qianyun Instrument Technology Co ltd
Priority date: 2020-10-20
Filing date: 2020-10-20
Publication date: 2021-06-25
Anticipated expiration: 2030-10-20

Abstract

The utility model provides a multifunctional bioreactor. The multifunctional bioreactor comprises a top cover and a base; the plurality of superposed layers are movably arranged between the top cover and the base and are movably connected; the culture solution inlet is formed in the top cover; the needle head filter is fixedly arranged on the top cover; the air inlet is formed in the top cover and communicated with the needle head filter; the overflow shell is fixedly arranged in the stacking layer; the overflow port is formed in the inner wall of the bottom of the superposed layer and corresponds to the overflow shell; the groove is formed in the top of the base. The utility model provides a multifunctional bioreactor has convenient to use, space utilization efficiency is high, the dust is convenient for the advantage of clearance.

Description

Multifunctional bioreactor

Technical Field

The utility model relates to the field of biotechnology, especially, relate to a multifunctional bioreactor.

Background

The bioreactor refers to a reaction system in which a liquid phase or a solid phase is inoculated with naturally occurring microorganisms or microorganisms having a specific degradation ability. The traditional bioreactor has the advantages of non-adjustable reaction volume, strong use limitation and common cell culture effect.

Therefore, there is a need to provide a new multifunctional bioreactor to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

The technical problem solved by the utility model is to provide a multifunctional bioreactor which is convenient to use, adjustable in volume and flexible to use.

In order to solve the above technical problem, the utility model provides a multifunctional bioreactor includes: a top cover and a base; the plurality of superposed layers are movably arranged between the top cover and the base and are movably connected; the culture solution inlet is formed in the top cover; the needle head filter is fixedly arranged on the top cover; the air inlet is formed in the top cover and communicated with the needle head filter; the overflow shell is fixedly arranged in the stacking layer; the overflow port is formed in the inner wall of the bottom of the superposed layer and corresponds to the overflow shell; the groove is formed in the top of the base; the exhaust pipe is fixedly arranged on the base and communicated with the groove; the liquid discharge pipe is fixedly installed on the base, communicated with the groove and lower than the exhaust pipe.

Preferably, a plurality of positioning columns are fixedly mounted on the top of the base and the top of the stacking layer, positioning grooves are formed in the bottom of the top cover and the bottom of the stacking layer, and the positioning grooves are matched with the positioning columns.

Preferably, the top of the base and the top of the superposed layer are both fixedly provided with sealing gaskets.

Preferably, the equal fixed mounting in both sides of top cap and stack layer has the bayonet lock, the equal fixed mounting in both sides of base and stack layer has the installation piece, slidable mounting has the slide bar on the installation piece, the sliding sleeve is equipped with the spring on the slide bar, the both ends of spring respectively with installation piece and slide bar fixed connection.

Preferably, the top end of the sliding rod is fixedly provided with an elastic sheet, the elastic sheet is provided with a clamping hole, and the clamping hole is matched with the corresponding clamping pin.

Preferably, the mounting block is provided with a sliding hole, and the inner wall of the sliding hole is in sliding connection with the sliding rod.

Preferably, the sliding rod is fixedly provided with a limiting block.

Compared with the prior art, the utility model provides a multifunctional bioreactor has following beneficial effect:

1) the middle superposed layer can be increased or reduced at will, the culture volume can be adjusted, and the use is convenient and flexible;

2) in the process of cell culture, particularly for the in vitro culture of adherent cells, a larger culture space can be obtained in an effective space, and the method is suitable for a large amount of culture application;

3) the air circulation of the culture room is improved, the communication between the culture solution and the gas is facilitated, the nutrient absorption is improved, and the cell growth is promoted;

4) the gas can flow in the culture chamber, the exhaust gas released in the cell growth process is promoted to be discharged quickly, the entry of fresh air is promoted, and the cell growth is promoted;

5) the culture solution is convenient to add and replace;

6) the overflow shells and the overflow ports are arranged in the superposed layers, so that the heights of the culture solution in the superposed layers and the culture environment are almost consistent, and the consistency of the culture effects of different layers is ensured;

7) the automatic perfusion of the culture solution and the automatic recovery of the waste liquid can be realized, the continuous and automatic dynamic perfusion culture of the culture solution is realized, the nutrients are increased, and the cell growth is promoted;

8) the cells can be collected simply by adding digestive juice to separate the cells from the culture plate and then collecting the cells through a waste liquid collecting port, or the bioreactor can be disassembled, and each layer is taken down and collected in layers;

9) each layer of the biological reaction vessel can be disassembled, and the cleaning, the sterilization and the maintenance are more convenient.

Drawings

FIG. 1 is a schematic structural view of a preferred embodiment of the multi-functional bioreactor of the present invention;

FIG. 2 is an enlarged view of part A of FIG. 1

FIG. 3 is a schematic top view of the stacked layers of FIG. 1;

FIG. 4 is a perspective view of the base shown in FIG. 1;

fig. 5 is a schematic top view of the top cover shown in fig. 1.

Reference numbers in the figures: 1. a top cover; 2. a base; 3. an overlying layer; 4. a culture solution inlet; 5. a needle filter; 6. an air inlet; 7. an overflow shell; 8. an overflow port; 9. a groove; 10. an exhaust pipe; 11. a liquid discharge pipe; 12. a positioning column; 13. positioning a groove; 14. a gasket; 15. a bayonet lock; 16. mounting blocks; 17. a slide bar; 18. a spring; 19. a spring plate; 20. and (6) clamping the hole.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and embodiments.

Referring to FIGS. 1-5 in combination, a multi-functional bioreactor comprises: a top cover 1 and a base 2; the multiple superposed layers 3 are movably arranged between the top cover 1 and the base 2, and the multiple superposed layers 3 are movably connected; the culture solution inlet 4 is formed in the top cover 1, and the culture solution inlet 4 is formed in the bottom cover 1; the needle head filter 5 is fixedly arranged on the top cover 1; the air inlet 6 is formed in the top cover 1, and the air inlet 6 is communicated with the needle filter 5; the overflow shell 7 is fixedly arranged in the superimposed layer 3; the overflow port 8 is formed in the inner wall of the bottom of the superposed layer 3, and the overflow port 8 corresponds to the overflow shell 7; the groove 9 is formed in the top of the base 2; the exhaust pipe 10 is fixedly arranged on the base 2, and the exhaust pipe 10 is communicated with the groove 9; the liquid discharge pipe 11 is fixedly arranged on the base 2, the liquid discharge pipe 11 is communicated with the groove 9, and the liquid discharge pipe 11 is lower than the exhaust pipe 10.

The top of base 2 and the top of superimposed layer 3 all fixed mounting have a plurality of reference columns 12, constant head tank 13 has all been seted up to the bottom of top cap 1 and the bottom of superimposed layer 3, constant head tank 13 and reference column 12 looks adaptation.

And sealing gaskets 14 are fixedly arranged on the top of the base 2 and the top of the superposed layer 3.

Top cap 1 and the equal fixed mounting in both sides of superimposed layer 3 have bayonet lock 15, the equal fixed mounting in both sides of base 2 and superimposed layer 3 has installation piece 16, slidable mounting has slide bar 17 on installation piece 16, the sliding sleeve is equipped with spring 18 on slide bar 17, spring 18's both ends respectively with installation piece 16 and slide bar 17 fixed connection.

An elastic sheet 19 is fixedly installed at the top end of the sliding rod 17, a clamping hole 20 is formed in the elastic sheet 19, and the clamping hole 20 is matched with the corresponding clamping pin 15.

The mounting block 16 is provided with a sliding hole, and the inner wall of the sliding hole is connected with the sliding rod 17 in a sliding manner.

And a limiting block is fixedly arranged on the sliding rod 17.

The utility model provides a multi-functional bioreactor's theory of operation as follows:

when the device is used, the culture solution is injected into the superposed layer 3 through the culture solution inlet 4, enters the next superposed layer 3 through the overflow port 8 after the liquid level of the culture solution is higher than the overflow shell 7, then flows into the groove 9 of the base 2, is discharged through the liquid discharge pipe 11, enters the superposed layer 3 after being filtered through the needle head filter 5 through air, flows in the superposed layer 3, and finally is discharged through the exhaust pipe 10, so that cell culture can be carried out;

when the superposed layers 3 are added, one superposed layer 3 is arranged on the superposed layer 3 on the uppermost layer, the sliding rod 17 is pressed, the clamping hole 20 on the elastic sheet 19 is clamped with the clamping pin 15, and the superposed layers 3 can be tightly and hermetically connected under the action of the elastic force of the spring 18.

5) the culture solution is convenient to add and replace;

6) the overflow shell 7 and the overflow port 8 are arranged in the superimposed layer 3, so that the height of the culture solution in the superimposed layer 3 and the culture environment are almost consistent, and the consistency of the culture effects of different layers is ensured;

The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.

Claims

1. A multi-functional bioreactor, comprising:

a top cover and a base;

the plurality of superposed layers are movably arranged between the top cover and the base and are movably connected;

the culture solution inlet is formed in the top cover;

the needle head filter is fixedly arranged on the top cover;

the air inlet is formed in the top cover and communicated with the needle head filter;

the overflow shell is fixedly arranged in the stacking layer;

the overflow port is formed in the inner wall of the bottom of the superposed layer and corresponds to the overflow shell;

the groove is formed in the top of the base;

the exhaust pipe is fixedly arranged on the base and communicated with the groove;

the liquid discharge pipe is fixedly installed on the base, communicated with the groove and lower than the exhaust pipe.

2. The multifunctional bioreactor according to claim 1, wherein a plurality of positioning posts are fixedly mounted on the top of the base and the top of the stacking layer, positioning grooves are formed in the bottom of the top cover and the bottom of the stacking layer, and the positioning grooves are matched with the positioning posts.

3. The multifunctional bioreactor according to claim 1, wherein the top of the base and the top of the superimposed layer are both fixedly provided with a sealing gasket.

4. The multifunctional bioreactor according to claim 1, wherein the top cover and the overlapping layer are fixedly provided with clamping pins at both sides, the base and the overlapping layer are fixedly provided with mounting blocks at both sides, the mounting blocks are provided with sliding rods in a sliding manner, the sliding rods are provided with springs in a sliding manner, and the two ends of the springs are respectively fixedly connected with the mounting blocks and the sliding rods.

5. The multifunctional bioreactor according to claim 4, wherein a spring plate is fixedly mounted at the top end of the sliding rod, a clamping hole is formed in the spring plate, and the clamping hole is matched with the corresponding clamping pin.

6. The bioreactor of claim 4, wherein the mounting block has a sliding hole, and the inner wall of the sliding hole is slidably connected to the sliding rod.

7. The multifunctional bioreactor according to claim 4, wherein a stopper is fixedly mounted on the sliding rod.