Liquid feeding auxiliary device for cell culture plate
Technical Field
The utility model relates to a biological utensil technical field, more specifically say, relate to a cell culture plate is with liquid feeding auxiliary device.
Background
The cell culture plate is an important consumable frequently used in the cell related experiment process, is usually made of transparent high polymer material polystyrene (GPPS), the surface of the cell culture plate is coated with a hydrophilic coating, the adherent growth of cells is facilitated, the cell culture plate can be prevented from being polluted by bacteria in the cell culture process through gamma ray sterilization treatment, and the cell culture plate can be divided into a 6-hole plate, a 12-hole plate, a 24-hole plate, a 48-hole plate, a 96-hole plate, a 384-hole plate and a 1536-hole plate according to the number of culture holes; the culture plate can be divided into a flat bottom culture plate and a round bottom culture plate (U-shaped and V-shaped) according to the shape of the bottom of the culture plate; the cell culture plate can be divided into a Terasaki plate and a common cell culture plate according to the material, and different types of culture plates can be used for different cell experiments.
At present, when a cell culture plate with a large number of holes is filled, a liquid transfer machine and a liquid transfer pipe are often used for simultaneously filling the holes in the same row, however, due to the fact that the inner diameter of the holes in the cell culture plate is small, and a corresponding auxiliary device is not needed during liquid filling, a worker is prone to leakage in the actual liquid filling process, and in addition, when the liquid filling process is interrupted (for example, liquid medicine in the liquid transfer machine is used up and needs to be supplemented), if liquid medicine originally exists in the holes of the cell culture plate, and the color of the liquid to be filled is similar to that of the existing liquid medicine, the worker is difficult to distinguish the holes filled with liquid from the holes not filled with liquid medicine, and the problems of liquid leakage or repeated liquid filling are prone to occur.
Disclosure of Invention
1. Technical problem to be solved
To the problem that exists among the prior art, the utility model aims to provide a cell culture is liquid feeding auxiliary device for board, it can realize playing support, stable effect to the pipette at the staff to cell culture board liquid feeding in-process, effectively avoids appearing spilling the problem of leaking, can also make things convenient for the staff to differentiate the hole of liquid feeding and the hole of liquid feeding not simultaneously, effectively avoids leaking the problem of liquid feeding or repeated liquid feeding.
2. Technical scheme
In order to solve the above problems, the utility model adopts the following technical proposal.
A liquid feeding auxiliary device for a cell culture plate comprises a base, wherein a containing groove is formed in the upper end of the base, a pair of sliding seats is connected to the upper end of the base in a sliding mode, the two sliding seats are respectively positioned on the front side and the rear side of the containing groove, a support block is fixedly connected to the upper end of each sliding seat, a stabilizing rod is fixedly connected between the two support blocks, a stabilizing groove is formed in the outer end of each stabilizing rod in a chiseling mode, a limiting rod positioned on the left side of the support block is arranged on the upper side of each sliding seat, through holes matched with the limiting rods are formed in the outer end of each sliding seat in a chiseling mode, a plurality of clamping grooves matched with the limiting rods are fixedly connected to the upper end of the base, the lower end of each limiting rod penetrates through the through holes and extends into the clamping grooves in the corresponding position of the limiting rod, an annular block, can realize playing support, stable effect to the pipette at the staff to cell culture board liquid feeding in-process, effectively avoid appearing spilling the problem of leaking, can also make things convenient for the staff to distinguish the hole of liquid feeding and the hole of liquid feeding not simultaneously, effectively avoid leaking the problem of liquid feeding or repeated liquid feeding.
Furthermore, the outer end of the limiting rod is fixedly connected with a limiting sliding block, a limiting sliding groove connected with the limiting sliding block in a sliding mode is formed in the inner wall of the through hole, the limiting sliding block and the limiting sliding groove are matched with each other, and the situation that a worker pulls the limiting rod out of the through hole in the sliding seat is effectively avoided.
Furthermore, the T-shaped sliding block is fixedly connected to the lower end of the sliding seat, a T-shaped guide groove in sliding connection with the T-shaped sliding block is formed in the upper end of the base, and the T-shaped sliding block and the T-shaped guide groove are matched with each other to play a role in limiting the moving track of the sliding seat.
Further, two fixedly connected has the apron of level setting between the slide, and the apron is located the stabilizer bar left side, and the apron can cover the hole on the cell culture plate that the liquid feeding was accomplished, effectively avoids downthehole receiving the pollution.
Furthermore, the cover plate is made of transparent glass materials, so that workers can observe the conditions in the upper hole of the cell culture plate conveniently, and can wipe and clean the cell culture plate conveniently.
Further, the base upper end is dug and is had a pair of groove of getting that is linked together with the holding tank, and two are got a groove and are located the holding tank left and right sides respectively, get a groove and can make things convenient for the staff to put into the holding tank with the cell culture board, also can make things convenient for the staff to take out the cell culture board from holding the inslot.
3. Advantageous effects
Compared with the prior art, the utility model has the advantages of:
(1) this scheme can realize playing support, stable effect to the pipette at the staff to cell culture board liquid feeding in-process, effectively avoids appearing spilling the problem of leaking, can also make things convenient for the staff to differentiate the hole of liquid feeding and the hole of not liquid feeding simultaneously, effectively avoids leaking the problem of liquid feeding or repeated liquid feeding.
(2) The limiting slide block is fixedly connected to the outer end of the limiting rod, the limiting slide groove is connected with the limiting slide block in a sliding mode through the inner wall of the through hole, the limiting slide block and the limiting slide groove are matched with each other, and the situation that a worker pulls the limiting rod out of the through hole in the slide seat is effectively avoided.
(3) The T-shaped sliding block is fixedly connected to the lower end of the sliding seat, the T-shaped guide groove in sliding connection with the T-shaped sliding block is formed in the upper end of the base, and the T-shaped sliding block and the T-shaped guide groove are matched with each other to play a role in limiting the moving track of the sliding seat.
(4) The cover plate is fixedly connected between the two sliding seats and horizontally arranged, the cover plate is positioned on the left side of the stabilizing rod, the cover plate can cover holes in the cell culture plate which are filled with liquid, and pollution in the holes is effectively avoided.
(5) The cover plate is made of transparent glass materials, so that the condition that workers observe the upper holes of the cell culture plate can be conveniently observed, and the workers can conveniently wipe and clean the cell culture plate.
(6) The base upper end is dug and is had a pair of groove of getting that is linked together with the holding tank, and two get a groove and be located the holding tank left and right sides respectively, get a groove and can make things convenient for the staff to put into the holding tank with the cell culture board in, also can make things convenient for the staff to take out the cell culture board from holding the inslot.
Drawings
Fig. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic cross-sectional view taken at A-A of FIG. 1;
FIG. 3 is a schematic cross-sectional view taken at B-B of FIG. 2;
fig. 4 is a schematic structural diagram at C in fig. 3.
The reference numbers in the figures illustrate:
the device comprises a base 1, a containing groove 2, a sliding seat 3, a support block 4, a stabilizing rod 5, a stabilizing groove 6, a limiting rod 7, a clamping groove 8, a ring-shaped block 9, an extension spring 10, a connecting rod 11, a limiting sliding block 12, a limiting sliding groove 13, a sliding block 14T, a guide groove 15T, a cover plate 16 and a workpiece taking groove 17.
Detailed Description
The technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiment of the present invention; obviously, the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention based on the embodiments of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "sleeved/connected", "connected", and the like are to be understood in a broad sense, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1:
referring to fig. 1-2, a liquid feeding auxiliary device for a cell culture plate comprises a base 1, wherein an accommodating groove 2 is formed in the upper end of the base 1, the accommodating groove 2 is matched with the cell culture plate and can be used for positioning the cell culture plate, a pair of sliding seats 3 are slidably connected to the upper end of the base 1, the two sliding seats 3 are respectively arranged on the front side and the rear side of the accommodating groove 2, the sliding seats 3 can slide left and right on the base 1, supporting blocks 4 are fixedly connected to the upper end of the sliding seats 3, stabilizing bars 5 are fixedly connected between the two supporting blocks 4 and are arranged right above the cell culture plate in the accommodating groove 2, stabilizing grooves 6 are formed in the outer ends of the stabilizing bars 5 in a chiseled mode, the specific shapes of the stabilizing grooves 6 are matched with pipettes, as shown in fig. 1-2, and the number and the positions.
Referring to fig. 2-3, a limiting rod 7 located on the left side of the supporting block 4 is disposed on the upper side of the sliding seat 3, referring to fig. 4, a through hole matched with the limiting rod 7 is drilled at the outer end of the sliding seat 3, a plurality of slots 8 matched with the limiting rod 7 are fixedly connected to the upper end of the base 1, the lower end of the limiting rod 7 penetrates through the through hole and extends into the corresponding slot 8, the slots 8 are specifically distributed as shown in fig. 1, the sliding seat 3 can be clamped and fixed on the base 1 by the cooperation between the limiting rod 7 and the slots 8, the outer end of the limiting rod 7 is fixedly connected with a ring block 9 located on the upper side of the sliding seat 3, an extension spring 10 is fixedly connected between the ring block 9 and the sliding seat 3, the extension spring 10 is sleeved outside the limiting rod 7, the ring block 9 and the extension spring 10 are matched with each other, the limiting rod 7 can be pulled downwards, referring to fig. 1-3, connecting rod 11 can be as an organic whole with two gag lever posts 7 connection to make things convenient for two gag lever posts 7 of staff's synchronous pulling, can realize playing support, stable effect to the pipette at the staff to cell culture board liquid feeding in-process, effectively avoid appearing spilling the problem of leaking, can also make things convenient for the staff to differentiate the hole of liquid feeding and the hole of not liquid feeding simultaneously, effectively avoid leaking the problem of liquid feeding or repeated liquid feeding.
Referring to fig. 4, the outer end of the limit rod 7 is fixedly connected with a limit slider 12, the inner wall of the through hole is provided with a limit chute 13 slidably connected with the limit slider 12, the limit slider 12 and the limit chute 13 are matched with each other, so that the situation that a worker pulls the limit rod 7 out of the through hole on the slide carriage 3 is effectively avoided, referring to fig. 1 and 4, the lower end of the slide carriage 3 is fixedly connected with a T-shaped slider 14, the upper end of the base 1 is provided with a T-shaped guide groove 15 slidably connected with the T-shaped slider 14, and the T-shaped slider 14 and the T-shaped guide groove 15 are matched with each other, so that the moving track of the slide carriage 3 can be.
Please refer to fig. 1 and 4, a cover plate 16 horizontally arranged is fixedly connected between the two sliding seats 3, the cover plate 16 is located on the left side of the stabilizer bar 5, the cover plate 16 can cover the hole on the cell culture plate after the liquid is added, and effectively avoid the pollution in the hole, the cover plate 16 is made of transparent glass material, which not only facilitates the staff to observe the situation in the hole on the cell culture plate, but also facilitates the staff to clean and clean the hole, please refer to fig. 1-2, a pair of taking grooves 17 communicated with the holding groove 2 is drilled at the upper end of the base 1, the two taking grooves 17 are respectively located at the left side and the right side of the holding groove 2, and the taking grooves 17 can facilitate the staff to put the cell culture plate into the holding groove 2, and also facilitate the staff to take the cell culture plate out of the holding groove 2.
Referring to fig. 1-4, when a worker needs to add liquid to a cell culture plate, the cell culture plate can be placed into the accommodating groove 2 on the base 1, the accommodating groove 2 can position the cell culture plate, then the worker can pull the connecting rod 11 upwards, the connecting rod 11 can drive the two limiting rods 7 to move upwards, so that the limiting rods 7 move out of the clamping grooves 8 at the corresponding positions, thereby releasing the clamping fixation of the sliding seats 3, and then the worker can move the two sliding seats 3 through the connecting rod 11, so that the stabilizing rod 5 moves to a position matched with the first row of holes at the left side of the cell culture plate, and then the worker can release the connecting rod 11, so that the two limiting rods 7 can move downwards under the mutual matching of the annular block 9 and the extension spring 10, so that the limiting rods 7 are combined with the clamping grooves 8 at the position, thereby fixing the sliding seats 3 and the stabilizing rods 5 thereon at the position,
referring to fig. 1-4, then, the worker can use the pipette and the pipette to fill liquid, during the liquid filling process, the worker can place the pipette into the stable groove 6 at the corresponding position and lean the pipette against the inner wall of the stable groove 6, so that the stable groove 6 on the stabilizer bar 5 can support and stabilize the pipette, thereby effectively avoiding the leakage problem, after the liquid filling of the first row of holes on the left side of the cell culture plate is completed, the worker can repeat the above operations to move the stabilizer bar 5 to the position matching with the second row of holes, thereby filling the second row of holes, and thereafter, repeat the above steps continuously until all the holes on the cell culture plate are filled with liquid, in addition, when the liquid filling is interrupted during the liquid filling process (for example, when the liquid in the pipette is used up and needs to be replenished), because the position of the stabilizer bar 5 is always matched with the position of the hole for liquid filling, consequently, when the staff carries out the liquid feeding once more, can judge which hole has the liquid feeding rapidly according to stabilizer bar 5's position, which hole has not the liquid feeding, thereby effectively avoid leaking the problem of liquid feeding or repeated liquid feeding, to sum up, this scheme can realize at the staff to the cell culture board liquid feeding in-process, play the support to the pipette, stable effect, effectively avoid appearing spilling the problem of leaking, simultaneously can also make things convenient for the staff to differentiate the hole of liquid feeding and the hole of not liquid feeding, effectively avoid leaking the problem of liquid feeding or repeated liquid feeding.
The above description is only the preferred embodiment of the present invention; the scope of the present invention is not limited thereto. Any person skilled in the art should also be able to cover the technical scope of the present invention by replacing or changing the technical solution and the improvement concept of the present invention with equivalents and modifications within the technical scope of the present invention.