CN220951843U - Synchronous manipulator for multicellular culture bottle - Google Patents

Abstract

The utility model belongs to the technical field of cell culture, in particular to a multi-cell culture bottle synchronous manipulator which comprises a plurality of culture square bottles, wherein bottle racks are arranged at the bottoms of the culture square bottles; the bottle rack is provided with a parallel moving plate and a fixed plate on one side facing the culture square bottle, the fixed plate is fixedly connected to the bottle rack, and the moving plate is connected to the bottle rack in a sliding way; a plurality of groups of limiting grooves with different depths are formed in one side, away from the bottle rack, of the movable plate and the fixed plate; a baffle is connected in the limiting groove in a clamping way, and the baffle simultaneously clamps the moving plate and the fixed plate to fix the interval between the moving plate and the fixed plate; the plurality of square bottles for culture are limited on the side wall of the bottle rack through the baffle plate. According to the utility model, through the structural design that the parallel moving plate and the fixing plate are arranged on one side of the bottle rack, the function of tightly connecting and fixing a plurality of culture square bottles on one side of the bottle rack through the moving plate and the fixing plate is realized, the problem that a plurality of culture bottles are difficult to operate in batches in the prior art is solved, and the working efficiency of cell passage and transfer is improved.

Description

Synchronous manipulator for multicellular culture bottle

Technical Field

The utility model relates to the technical field of cell culture, in particular to a synchronous manipulator for a multicellular culture bottle.

Background

When biological products such as cells and enzymes are transferred, the biological products are sensitive to concussion and high turbulence, and mild operation is required. In transferring in a flask, it is necessary to tilt the flask to allow the transfer liquid to slowly flow down the flask to ensure that the biological components in the transfer liquid are not deactivated by overdriving operations and to avoid the formation of large numbers of bubbles during transfer.

The cell passage and transfer process is a number of repeated efforts, first the solution is slowly added to the flask along the bottom of the flask, then the flask is erected to check the liquid loading and prevent premature cell attachment, and then the flask is horizontally placed for culture to allow cell attachment to grow. Operational differences in the transfer process may affect the state of the cultured cells and the growth curve, and thus the results of subsequent experiments.

One patent, issued to the trade name CN212982960U, discloses a support is placed in slope of blake bottle, solvent bottle, through placing the blake bottle in placing the section of thick bamboo, rotates the hand wheel for the carriage release lever upwards moves and will place the jack-up of swivel plate one side, makes the blake bottle slope certain angle.

Although this patent has realized the function that slope blake bottle slope set up, but the interval distance between a plurality of placing drums is too big, leads to the blake bottle to be difficult to closely arrange, and the multicellular blake bottle batch synchronization operation of being inconvenient for to the blake bottle is placed in a section of thick bamboo and is inconvenient for operating personnel to observe the liquid level height.

Disclosure of utility model

Based on the above, it is necessary to provide a multi-cell culture flask synchronous manipulator for solving the problems that a plurality of culture flasks are difficult to be closely arranged and difficult to operate in batch in the prior art.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

The utility model relates to a synchronous manipulator for multicellular culture bottles, which comprises a plurality of culture square bottles, wherein bottle racks are arranged at the bottoms of the culture square bottles; the bottle rack is provided with a parallel moving plate and a fixed plate on one side facing the culture square bottle, the fixed plate is fixedly connected to the bottle rack, and the moving plate is connected to the bottle rack in a sliding way; a plurality of groups of limiting grooves with different depths are formed in one side, away from the bottle rack, of the movable plate and the fixed plate; a baffle is connected in the limiting groove in a clamping way, and the baffle simultaneously clamps the moving plate and the fixed plate to fix the interval between the moving plate and the fixed plate; the plurality of square bottles for culture are limited on the side wall of the bottle rack through the baffle plate.

Further, two parallel guide grooves are formed in the length direction of the side wall of the bottle rack; two groups of guide posts are arranged at positions of the movable plate corresponding to the guide grooves, and the movable plate is slidably connected in the guide grooves through the guide posts.

Further, a plurality of groups of corresponding fixing holes are formed at the edges of the two sides of the moving plate and the fixing plate, and the plurality of fixing holes are linearly distributed along the edges; the liquid level lines are inserted into the fixing holes at the edges of the movable plate and the fixing plate, so that the liquid level scale lines of the culture square bottle are formed.

Further, one side of the baffle plate facing the bottle rack is linearly provided with a plurality of tooth grooves, and the opening of each tooth groove is the same as the thickness of the moving plate; the baffle is connected in a limit groove on the movable plate through tooth groove clamping.

Further, the baffle extends outwards to form a support column facing the fixed plate; when the bottle rack is vertical to the operation table top, the end part of the support column is contacted with the operation table top.

Further, a base is arranged at the bottom of the bottle rack; one end of the base is provided with a rotating shaft, and the base is rotationally connected with one end of the bottle rack through the rotating shaft.

Further, one end of the bottle rack facing the rotating shaft is provided with a clamping hook; the clamping hook is semicircular, the inner cambered surface of the clamping hook is clamped and connected to the outer peripheral wall of the rotating shaft, and the bottle rack is rotatably connected to the rotating shaft through clamping hook clamping.

Further, a plurality of clamping grooves are formed in the top of the base at equal intervals along the length direction of the base, and the clamping grooves are obliquely arranged; the bottle rack is provided with the telescopic link towards base one side, and telescopic link one end rotates with the bottle rack bottom to be connected, and the other end is pegged graft in the draw-in groove.

Further, the telescopic link is formed by two sections of long rods in a sleeved connection mode, and an adjusting bolt used for fixing the length of the telescopic link is arranged at the joint.

Compared with the prior art, the utility model has the beneficial effects that:

1. According to the utility model, through the structural design that the parallel moving plate and the fixing plate are arranged on one side of the bottle rack, the function of tightly connecting and fixing a plurality of culture square bottles on one side of the bottle rack through the moving plate and the fixing plate is realized, the problems that a plurality of culture bottles are difficult to be tightly distributed and are inconvenient for batch operation in the prior art are solved, and the working efficiency of cell passage and transfer is improved;

2. According to the utility model, through the structural design that the bottle rack is rotationally connected with the base through the rotating shaft, and the base is provided with the plurality of clamping grooves, the included angle between the culture bottle and the horizontal plane can be adjusted according to different process requirements, the uniform operation function can be ensured by normalizing the angle during operation, and the accuracy of the inclination adjustment of the culture bottle is improved.

Drawings

The disclosure of the present utility model is described with reference to the accompanying drawings. It should be understood that the drawings are for purposes of illustration only and are not intended to limit the scope of the present utility model in which like reference numerals are used to designate like parts.

Wherein:

FIG. 1 is a schematic perspective view of a synchronous manipulator for multicellular culture flasks according to the present utility model;

FIG. 2 is a schematic diagram of a moving plate structure according to the present utility model;

FIG. 3 is a schematic view of a hook structure according to the present utility model;

fig. 4 is a schematic view of a baffle structure in the present utility model.

The reference numerals in the drawings indicate: 11. a base; 12. a clamping groove; 13. a vacancy; 14. a rotating shaft; 15. culturing a square bottle; 21. a bottle holder; 22. a clamping hook; 23. a guide groove; 24. a moving plate; 25. a fixing plate; 31. a baffle; 32. a support column; 41. a telescopic rod; 42. an adjusting bolt; 51. a liquid level line; 52. a fixing hole; 61. a limit groove; 62. and a guide post.

Detailed Description

It is to be understood that, according to the technical solution of the present utility model, those skilled in the art may propose various alternative structural modes and implementation modes without changing the true spirit of the present utility model. Accordingly, the following detailed description and drawings are merely illustrative of the utility model and are not intended to be exhaustive or to limit the utility model to the precise form disclosed.

Referring to fig. 1-4, an embodiment of the present utility model is a multi-cell culture flask synchronization manipulator, which includes a base 11, a rotating shaft 14, a culture flask 15, a flask rack 21, a hook 22, a moving plate 24, a fixing plate 25, a baffle 31, a support column 32, a telescopic rod 41, an adjusting bolt 42, a liquid level line 51 and a guide column 62.

Referring to fig. 1, a plurality of culture square bottles 15 are sequentially placed on top of a bottle holder 21, and a moving plate 24 and a fixing plate 25 are disposed on a side of the bottle holder 21 facing the culture square bottles 15, both of which are perpendicular to the bottle holder 21. Wherein the fixed plate 25 is fixedly connected to the bottle holder 21, the movable plate 24 is slidably connected to the bottle holder 21, and the plurality of culture square bottles 15 are positioned between the movable plate 24 and the fixed plate 25. Two parallel guide grooves 23 are respectively arranged on the side plates on two sides of the bottle frame 21, two guide posts 62 are respectively arranged on two sides below the moving plate 24, and the two guide posts 62 are respectively inserted into the two parallel guide grooves 23, so that the moving plate 24 can move along the guide grooves 23 and ensure that the moving plate 24 is perpendicular to the bottle frame 21.

Referring to fig. 1 and 2, a plurality of groups of limiting grooves 61 with different depths are formed on the sides of the moving plate 24 and the fixed plate 25, which are away from the bottle rack 21, so as to adapt to culture square bottles 15 with different sizes and heights; the baffle 31 is provided with a plurality of tooth grooves linearly on one side facing the bottle rack 21, and the openings of the tooth grooves are the same as those of the movable plate 24; baffle 31 passes through the tooth's socket block and connects in the spacing groove 61 on movable plate 24 to the interval between fixed plate 25 and the fixed plate 24, and with the spacing at the top of bottle frame 21 of culture square bottle 15 block, avoid the skew of culture square bottle 15 to rock. When in use, the movable plate 24 is moved along the guide groove 23 to adjust the distance between the movable plate 24 and the fixed plate 25, and when the fixing is needed, the tooth grooves of the baffle 31 are engaged with the limit grooves 61 on the movable plate 24 and the fixed plate 25, so that a plurality of culture square bottles 15 are stacked and fixed on the bottle rack 21.

A plurality of groups of corresponding fixing holes 52 are formed at the edges of the two sides of the moving plate 24 and the fixing plate 25, and the plurality of fixing holes 52 are linearly distributed along the edges of the side walls. The liquid level line 51 is an elastic rope with fixing pins at two ends, and two ends of the liquid level line 51 are respectively inserted into corresponding fixing holes 52 on the moving plate 24 and the fixing plate 25. The two ends of the liquid level line 51 are fixed at the positions of different fixing holes 52 to adjust the height and the angle of the liquid level line 51, and the liquid level line 51 can be used as a reference scale line for observing the liquid level in the culture square bottle 15, and the pipetting accuracy is controlled and confirmed under the condition of not adjusting the position of an operator.

Referring to fig. 3 and 4, the base 11 is located at the bottom of the bottle rack 21, a plurality of slots 12 are formed in the top of the base 11, the slots 12 are equidistantly arranged along the length direction of the base 11, and the openings of the slots 12 are inclined toward one side. A rotating shaft 14 is arranged at one end of the base 11, the rotating shaft 14 is rotatably arranged on the base 11, a gap is formed between the periphery of the rotating shaft 14 and the top surface of the base 11, and a gap 13 is formed between the rotating shaft 14 and the clamping groove 12.

The clamping hook 22 is arranged at one end of the bottle rack 21, the clamping hook 22 is in a semicircular ring structure, and the intrados of the clamping hook 22 can be clamped and connected with the extrados of the rotating shaft 14 and can be tightly attached to the extrados to rotate, so that the bottle rack 21 can rotate along the rotating shaft 14 to gently mix liquid in the square culture bottle 15. And the bottle rack 21 is convenient to detach in a clamping and rotating connection mode of the clamping hooks 22, the clamping hooks 22 are vertically inserted into the gaps 13, the inner cambered surfaces of the clamping hooks 22 are clamped and tightly attached to the outer cambered surfaces of the rotating shafts 14 in a translation mode, when the bottle rack 21 rotates towards the direction of the base 11 by taking the rotating shafts 14 as axes, the clamping hooks 22 are positioned below the rotating shafts 14 to rotate, and the clamping hooks 22 can be prevented from being separated from the rotating shafts 14.

Referring to fig. 3 and 4, a telescopic rod 41 is disposed below the middle of the bottle rack 21, one end of the telescopic rod 41 is rotatably connected to the bottom of the bottle rack 21 through a rotating rod shaft, the telescopic rod 41 is formed by sleeving and connecting two sections of long rods, and an adjusting bolt 42 for fixing the length of the telescopic rod 41 is disposed at the joint. The telescopic rod 41 can rotate along the rotating shaft, when the bottom end is inserted into one clamping groove 12 on the base 11, the telescopic rod 41, the base 11 and the bottle rack 21 form a stable triangular structure, and at the moment, the bottle rack 21 and the base 11 can form one included angle of 0-90 degrees. When adjusted to the appropriate angle, the stacked flasks can be conveniently pipetted manually or by means of an appropriate rack.

The support column 32 is located at one end of the baffle 31 near the rotation shaft 14, the end of the baffle 31 extends outwards to form the support column 32, and when the bottle rack 21 is vertical to the base 11, the support column 32 at the bottom of the baffle 31 contacts with the operation table surface to support the bottle rack 21 without toppling. The synchronous manipulator described in this embodiment has a simple structure, is easy to operate, is provided with a liquid level line 51, and can facilitate the observation, confirmation and unification of the liquid feeding amount of each culture square bottle 15 by a technician. By rotating the bottle rack 21 along the rotation shaft 14, the square culture bottle 15 on the bottle rack 21 forms a certain inclination angle with the horizontal plane, so that the liquid can be ensured to slowly flow in along the bottle bottom without agitating a large number of bubbles during pipetting, and the stability of sensitive substances such as cells in the feed liquid is ensured. The included angle between the reagent bottle and the horizontal plane can be adjusted according to different process requirements through the design of the multiple clamping grooves 12, and the operation uniformity can be ensured by normalizing the angle during operation.

In the description of the present utility model, it should be understood that the terms "center," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate describing the present utility model and simplify the description, and do not indicate or imply that the devices or elements being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the scope of the present utility model.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (9)

1. A multicellular culture flask synchronous manipulator comprising a plurality of culture square flasks (15), characterized in that: the bottoms of the culture square bottles (15) are provided with bottle racks (21); the bottle rack (21) is provided with a moving plate (24) and a fixed plate (25) which are parallel to each other on one side facing the culture square bottle (15), the fixed plate (25) is fixedly connected to the bottle rack (21), and the moving plate (24) is slidably connected to the bottle rack (21); a plurality of groups of limiting grooves (61) with different depths are formed in one side, away from the bottle rack (21), of the moving plate (24) and the fixed plate (25); a baffle plate (31) is connected in the limiting groove (61) in a clamping way, and the baffle plate (31) is simultaneously clamped with the movable plate (24) and the fixed plate (25) to fix the distance between the movable plate and the fixed plate; the culture square bottles (15) are limited on the side wall of the bottle rack (21) through the baffle plates (31).

2. The synchronized manipulator of claim 1, wherein: two parallel guide grooves (23) are formed in the length direction of the side wall of the bottle rack (21); two groups of guide posts (62) are arranged at positions of the movable plate (24) corresponding to the guide grooves (23), and the movable plate (24) is connected in the guide grooves (23) in a sliding mode through the guide posts (62).

3. The synchronized manipulator of claim 1, wherein: a plurality of groups of corresponding fixing holes (52) are formed in the edges of the two sides of the moving plate (24) and the fixing plate (25), and the plurality of fixing holes (52) are linearly distributed along the edges; the liquid level lines (51) are inserted into the fixing holes (52) at the edges of the moving plate (24) and the fixing plate (25), so that the liquid level scale lines of the culture square bottle (15) are formed.

4. The synchronized manipulator of claim 1, wherein: a plurality of tooth grooves are linearly formed in one side of the baffle (31) facing the bottle rack (21), and the thickness of the opening of each tooth groove is the same as that of the movable plate (24); the baffle (31) is connected in a limit groove (61) on the movable plate (24) through tooth grooves in a clamping mode.

5. The synchronized manipulator of claim 1, wherein: the baffle (31) extends outwards to form a support column (32) facing the fixed plate (25); when the bottle holder (21) is perpendicular to the operation table, the end of the support column (32) is in contact with the operation table.

6. A multi-cell culture flask synchronization manipulator according to any one of claims 1-5, wherein: the bottom of the bottle rack (21) is provided with a base (11); one end of the base (11) is provided with a rotating shaft (14), and the base (11) is rotatably connected with one end of the bottle rack (21) through the rotating shaft (14).

7. The synchronized manipulator of claim 6, wherein: one end of the bottle rack (21) facing the rotating shaft (14) is provided with a clamping hook (22); the clamping hook (22) is semicircular, the inner cambered surface of the clamping hook (22) is connected with the outer circumferential wall of the rotating shaft (14) in a clamping mode, and the bottle rack (21) is connected to the rotating shaft (14) in a clamping and rotating mode through the clamping hook (22).

8. The synchronized manipulator of claim 6, wherein: the top of the base (11) is provided with a plurality of clamping grooves (12) at equal intervals along the length direction, and the clamping grooves (12) are obliquely arranged; the bottle rack (21) is provided with a telescopic rod (41) facing one side of the base (11), one end of the telescopic rod (41) is rotationally connected with the bottom of the bottle rack (21), and the other end of the telescopic rod is inserted into the clamping groove (12).

9. The synchronized manipulator of claim 8, wherein: the telescopic rod (41) is formed by sleeving and connecting two sections of long rods, and an adjusting bolt (42) for fixing the length of the telescopic rod (41) is arranged at the joint.