CN213302248U

CN213302248U - Sample detection device

Info

Publication number: CN213302248U
Application number: CN201821071363.4U
Authority: CN
Inventors: 周凯军; 洪亮; 王佐红; 陈宇杰; 吴淑江
Original assignee: Hangzhou Biotest Biotech Co Ltd
Current assignee: Hangzhou Biotest Biotech Co Ltd
Priority date: 2018-02-13
Filing date: 2018-07-07
Publication date: 2021-05-28
Anticipated expiration: 2028-07-07

Abstract

The invention provides a sample detection device, which is used for a second cavity for collecting a sample and a detection area for initially collecting the sample, wherein the detection area is closed when the sample is not collected in the second cavity or is collected in the second cavity, and the sample can enter the detection area when the collection of the second cavity is completed and sealed. The device can separate the initial detection sample and the detection sample of secondary confirmation, the initial sample of collecting through this detection device, can enter into first chamber and second intracavity respectively, then after the initial detection is accomplished or before accomplishing, the second chamber can be separated with initial collection chamber (first chamber), realize once collecting the secondary detection, can not pollute between the sample of initial detection and the sample of confirming the detection, the sample of confirming the detection just is sealed before the sample of initial detection gets into the detection zone, ensure that the sample of confirming the detection of secondary can not be polluted by the detection liquid of initial detection, influence the effect of secondary confirmation.

Description

Sample detection device

Technical Field

The present invention relates to a device for collecting a liquid sample, and more particularly to a device for collecting and detecting an analyte in a liquid sample, such as a urine collection and detection device, in the field of rapid diagnosis.

Background

Currently, a large number of test devices for detecting whether a sample contains an analyte are used in hospitals or homes, and these test devices for rapid diagnosis include one or more test reagent strips, such as an early pregnancy test, a drug abuse test, and the like. The rapid diagnosis test device is convenient, and can obtain the test result on the test reagent strip within one minute or at most ten minutes.

Drug detection is widely applied and is commonly used in drug-resistant departments, public security bureaus, drug rehabilitation centers, physical examination centers, national soldier physical examination places and other institutions. The drug detection urine cup has various drug detection types and frequent times, and has a huge market demand, and after the drug detection urine cup on the market finishes detection, the sample in the urine cup is polluted by the detection reagent and cannot be continuously used for secondary confirmation detection, for example, as described in U.S. patent 7300633.

Although, in the conventional technique, the sample to be tested can be isolated from the collected sample, it is costly and not easy to handle. For example, U.S. patent No. 7300633 describes a piston urine cup that allows a liquid sample, such as urine, in a collection chamber to be transferred from the collection chamber to a detection chamber during advancement of a piston, where a test element is located to detect an analyte in the sample, and the liquid sample in the collection chamber is separated by the piston so that the two samples do not mix, and can be used for subsequent confirmatory testing. Although the detected sample and the collected sample can be separated, the urine cup of the piston is high in cost and difficult to operate, and after all, the piston needs to be pushed by a large force, so that the sample needs to be transferred by the piston, the liquid sealing effect needs to be achieved with the wall of the piston, and the piston cavity need to be tightly combined to achieve the sealing effect.

For another example, U.S. patent 8992855 describes a device for collecting a liquid sample that includes a piston structure integral with and movable with a cap, and that, while allowing separation of the test sample from the collected sample, requires a large amount of pressure to be overcome to access the test sample after it has entered the test chamber, and requires the cap and cup to be precisely dimensioned so that the piston integral with the cap can be accurately inserted into the separation chamber.

In addition, after the initial tests of these conventional collecting and detecting devices are completed, if the subsequent confirmation tests are required, the whole collecting and detecting device needs to be transported to a confirmation and detection mechanism for further confirmation tests, which brings about many problems, at least such problems: first, most current liquid collection and testing devices are provided with only a preliminary testing chamber. If subsequent confirmation detection is required, the whole device containing the urine and the detection reagent strip can only be sent to a confirmation detection mechanism for detection. The sample in the urine cup may be contaminated with the detection reagent. Secondly, when the whole device is sent to the confirmation detection mechanism, the risk of liquid leakage exists in the transportation process due to the fact that the cup opening is large, and therefore more cost is needed to enable the device to have a better sealing effect, and the risk of leakage is reduced as much as possible; third, after the entire device is transported to the confirmation and inspection mechanism, the confirmation and inspection mechanism requires a large low-temperature warehouse to store the entire device, preventing the liquid sample from deteriorating, and preparing for possible further confirmation and inspection later, which causes a significant increase in the cost of the confirmation and inspection mechanism (which may be referred to as a secondary inspection mechanism).

In view of the above technical problems, it is desirable to improve the above and provide an alternative way to overcome the shortcomings of the conventional technologies.

Disclosure of Invention

In view of the above situation, to overcome the defects of the prior art, the present invention provides a sample detection device, which can separate an initial detection sample and a secondary confirmation detection sample, wherein the initial sample collected by the detection device can enter a first cavity and a second cavity respectively, and then after or before the initial detection is completed, the second cavity can be separated from the initial collection cavity (the first cavity), so as to achieve a primary collection secondary detection, wherein no contamination occurs between the initial detection sample and the confirmation detection sample, and the confirmation detection sample is sealed before the initial detection sample enters a detection area, so as to ensure that the secondary confirmation detection sample is not contaminated by the detection liquid of the primary detection, thereby affecting the secondary confirmation effect.

In order to solve the above technical problems, the present invention provides the following:

a sample testing device comprises a second cavity for collecting a sample and a testing area for initially collecting the sample, wherein when the second cavity does not collect the sample or collects the sample, the testing area is closed, and when the second cavity is completely collected and sealed, the sample can enter the testing area.

Further, a detection inlet is included, which can be opened or closed.

Further, a blocking element is included, which is capable of closing or opening the detection inlet.

Further, the detection inlet is opened when the second chamber is completely collected and sealed.

Further, a second sealing element for sealing the second cavity is included, the second sealing element being capable of opening or closing the detection inlet, and opening the detection inlet when the second sealing element completes the sealing of the second cavity.

Further, the second sealing element is linked with the blocking element, and the blocking element is triggered to open the inlet communicated with the detection area while the second sealing element completes the sealing of the second cavity.

Further, a first chamber for collecting an initial test sample is included, the first chamber being capable of being in fluid communication with or isolated from the test area.

Further, when the detection inlet is opened, the first cavity and the detection area are in liquid communication, and when the detection inlet is closed, the first cavity and the detection area are isolated.

Further, including the first sealing element who is used for sealed first chamber, first sealing element and the linkage of second sealing element, first sealing element can open or close and detect the entry, and when the second sealing element was accomplished to the sealed of second chamber, drive first sealing element and open and detect the entry.

Further, the first sealing element is linked with the blocking element, and the blocking element is triggered to open the inlet communicated with the detection area through the first sealing element while the second sealing element completes the sealing of the second cavity.

Further, still include the assembly passageway of second chamber and be used for the third sealing element of sealed this passageway, third sealing element and the linkage of second sealing element, third sealing element can open or close and detect the entry, and when second sealing element accomplished the sealed of second chamber, drive third sealing element and open and detect the entry.

Further, the third sealing element is in linkage with the blocking element, and the blocking element is triggered to open the inlet communicated with the detection area through the third sealing element while the second sealing element completes the sealing of the second cavity.

The following technical scheme can also belong to the content of the invention:

in a first aspect, the present invention provides a sample collection and testing device, which comprises a first chamber for collecting a liquid sample and a second chamber for collecting a sample for confirmation of detection, wherein the first chamber and the second chamber can be combined or separated, that is, the combination of the first chamber and the second chamber can be a detachable combination, so that the first chamber and the second chamber can be separated under certain conditions. In some preferred embodiments, the first chamber and the second chamber are combined in the initial state, and the first chamber and the second chamber are in fluid communication in the combined state, that is, the liquid sample to be collected can enter the first chamber and the second chamber at the same time, or enter the second chamber and the first chamber at the same time, so that the one-time collection can be realized without separately collecting the first chamber and the second chamber. In some preferred forms, the first chamber has an outward opening, and the opening of the second chamber communicates with the interior of the first chamber.

Since the first cavity and the second cavity have a process from combination to separation in the using process, in some preferred modes, the second cavity needs to be designed in a form capable of being partially separated from the first cavity, for example, a part of the second cavity can be exposed out of the outline of the first cavity, and further, in some preferred modes, as the second cavity is separated from the first cavity, the part of the second cavity exposed out of the first cavity can gradually exit from the space of the first cavity.

When the first cavity and the second cavity are in a combined state, only one of the cavities can be sealed, because the first cavity and the second cavity have a common opening, and after the first cavity and the second cavity are separated, the second cavity needs to be independently sealed, so that a sealing device which is independently used for the second cavity needs to be arranged.

In some preferred modes, the second cavity collects the sample through the first cavity, the second cavity can be detachably connected or combined with the first cavity, the second cavity is combined with the first cavity in the sample collection process, and the second cavity can be pushed out of the first cavity after the sample collection is completed.

In some preferred forms, the second chamber includes a second collection port for collecting the sample, the second collection port being capable of being in fluid communication with the interior of the first chamber when the second chamber collects the sample. In some preferred forms the second chamber is disposed at the bottom of the first chamber. In some preferred forms, the first chamber has a first collection port for collecting a sample. In some preferred modes, the opening directions of the first collecting port and the second collecting port are consistent.

In some preferred modes, the liquid sample can naturally enter the second cavity while the liquid sample is filled into the first cavity. In some preferred modes, the liquid sample can enter the second cavity under the action of external force while the liquid sample is filled into the first cavity.

In some preferred forms, the bottom of the first chamber is provided with a channel into which the second chamber can fit. In some preferred forms, the second chamber is movable within the channel. In some preferred forms, the second chamber and the passageway are detachably joined or connected. In some preferred forms, the first passage is in fluid communication with the interior of the first chamber. In some preferred forms, the second chamber fits within the channel when the second chamber channel collects the sample. In some preferred forms, the second chamber is capable of moving away from the first chamber under an external force after sample collection.

In a second aspect, the present invention provides specific sealing structures that may include a second sealing member for sealing the second chamber, and a third sealing member for sealing a separation of the first chamber and the second chamber when the first chamber and the second chamber are separated.

It should be noted that the first, second and third elements in the present invention do not indicate the actual number, for example, the present invention may only include the second and/or third elements, but not necessarily the first, or may only include the first and/or third elements, but not necessarily the second, or may only include the first and/or second elements, but not necessarily the third, and therefore, the first, second or third elements are added, and only for the convenience of description and the correspondence of the relationship between the components, and the first, second and third elements herein do not indicate the numbering or ordering according to the actual number.

In some preferred embodiments, the first sealing member and the opening portion of the first chamber are sealed by a first screw structure, the second sealing member and the opening portion of the second chamber are sealed by a second screw structure, and the third sealing member and the separation portion are sealed by a third screw structure. In some preferred forms, the sealing structures may further include a first sealing member for sealing the first chamber.

In some preferred forms, the first sealing element and the second sealing element are coupled, and the second sealing element may seal the second chamber during the sealing of the first chamber by the first sealing element, possibly when the sealing of the first sealing element is completed, the sealing of the second sealing element is completed, or before the sealing of the first sealing element is completed.

In some preferred forms, the first sealing element and the third sealing element are coupled, and the third sealing element may seal the separation site while the first sealing element seals the first chamber, possibly while the second sealing element completes sealing when the first sealing element completes sealing, or before the first sealing element completes sealing.

In some preferred forms, the second sealing element and the third sealing element are coupled, and the third sealing element may seal the separation site while the second sealing element seals the second chamber, and it is possible that the third sealing element also completes the sealing when the second sealing element completes the sealing, or the second sealing element completes the sealing before the third sealing element completes the sealing.

Since the first sealing element, the second sealing element and the third sealing element need to have a linkage relationship, some linkage structure must be provided between the sealing elements.

In a third aspect, the invention provides a linkage structure of sealing elements, comprising the sealing elements of the sample collection device, wherein linkage of the sealing elements is realized through a linkage element.

In some preferred modes, the linkage structure comprises a first sealing element for sealing the first cavity and a second sealing element for sealing the second cavity, and the first sealing element and the second sealing element are linked through the first linkage element. In some preferred forms, the linkage structure includes a first sealing element for sealing the first cavity and a third sealing element for sealing the passage, the first and third sealing elements being linked by the first linkage element. In some preferred forms, the linkage structure includes a second sealing element for sealing the second chamber and a third sealing element for sealing the passage, the second and third sealing elements being linked by a second linkage element. In some preferred modes, the sealing device comprises a first sealing element for sealing the first cavity, a second sealing element for sealing the second cavity and a third sealing element for sealing the channel, the first sealing element and the second sealing element are linked through a first linkage element, and the second sealing element and the third sealing element are linked through a second linkage element. In some preferred modes, the sealing device comprises a first sealing element for sealing the first cavity, a second sealing element for sealing the second cavity and a third sealing element for sealing the channel, the first sealing element and the second sealing element are linked through a first linkage element, and the second sealing element and the third sealing element are linked through tight fit or fixed connection.

In some preferred forms, the linkage between the second sealing element and the third sealing element may be by a fixed connection. In some preferred forms, the linkage between the first sealing element and the third sealing element may be by a fixed connection.

In some preferred forms, the first linkage element is a linkage rod. In some preferred forms, the second linkage element is a linkage pin. In some preferred modes, the linkage refers to synchronous rotation. In some preferred modes, the linkage refers to synchronous movement towards the direction close to the inside of the sample.

In some preferred modes, the first linkage element is a linkage rod, and two ends of the linkage rod are respectively connected with the first sealing element and the third sealing element, so that when one of the first sealing element and the third sealing element rotates, the other one of the first sealing element and the third sealing element can be driven to rotate along with the linkage rod, the rotation along with the linkage rod can be synchronous or intermittent, but the rotation along with the linkage rod is usually in the same direction. In some preferred modes, the inner cover surface of the first sealing element is provided with a shaft hole, and the linkage rod is inserted into the shaft hole. In some preferred modes, the outer cover surface of the third sealing element is provided with a shaft hole, and the linkage rod is inserted into the shaft hole. In some preferred forms, the linkage rod connects the first sealing element and the third sealing element through the first lumen.

In some preferred modes, the linkage rod has a certain shape, generally speaking, the shape is not a perfect circle and can be, for example, a square shape, a semicircular shape or a triangular shape, and correspondingly, the shaft holes on the first sealing element and the third sealing element are also the same shape, so that the linkage rod and the shaft holes are circumferentially limited through the shape and cannot rotate relatively, and therefore, the linkage rod can drive the first sealing element and the third sealing element to rotate through the two shaft holes, and linkage is realized. In other possible modes, the linkage rod and the shaft hole can also be round holes which are tightly matched, and the linkage force comes from the friction force between the linkage rod and the abutting surface of the shaft hole. In other possible modes, the linkage rod and the shaft hole can be in a stepping linkage fit relation, under the stepping linkage fit relation, the linkage rod or the shaft hole can independently rotate for a certain angle and then synchronously rotate, the first sealing element can seal the first cavity while rotating, and the third sealing element can seal the separation part in the process of sealing the first cavity by the first sealing element.

As another realization form of linkage matching, a shaft hole is arranged on the linkage rod, a matching element linked with the shaft hole is arranged on the first sealing element or the third sealing element, the matching element and the shaft hole have the same shape, and the shape deviates from the axis of the linkage rod or is non-circular, so that the linkage rod and the matching element can realize linkage. In some preferred modes, the linkage rod can also be matched with the matching element in a stepping type linkage mode.

In some preferred forms, the linkage structure may further include a second linking member for linking the second sealing member and the third sealing member. The second linkage element is a linkage pin or a short object with a similar shape, the linkage pin can be fixedly connected on one of the second sealing element or the third sealing element, a linkage hole matched with the linkage pin is arranged on the other one of the second sealing element or the third sealing element, similarly, the linkage pin and the linkage hole also have certain shapes, the shapes are similar to the linkage rod and can deviate from the rotation center of the second sealing element or the third sealing element or are non-orthocircular, the shapes can be square, semicircular or triangular, and the like, so that one of the linkage pin and the linkage hole can drive the other one to rotate along with the linkage pin and the linkage hole, the rotation can be synchronous or discontinuously driven, the rotation can be in the same direction or in the opposite direction, the third sealing element can seal a separation part while rotating, and in the process of sealing the separation part by the third sealing element, the second sealing element may seal the second cavity.

As a specific implementation form of linkage matching, a linkage hole is formed in the inner cover surface of the third sealing element, the linkage pin is inserted into the linkage hole, and the other end of the linkage pin is fixedly connected to the outer cover surface of the second sealing element, or a linkage hole is formed in the outer cover surface of the second sealing element, one end of the linkage pin is inserted into the linkage hole, and the other end of the linkage pin is fixedly connected to the inner cover surface of the third sealing element. In this implementation, the linkage of the second sealing element and the first sealing element is transmitted through the third sealing element, that is, the third sealing element can seal the separation point while the second sealing element can seal the second cavity in the process of sealing the first cavity by the first sealing element.

In some preferred manners, the second sealing element and the third sealing element may not be provided with the second linkage element, but realize linkage through self structural cooperation, for example, certain surfaces of the second sealing element and the third sealing element are fixedly connected together, so that the two sealing elements can synchronously rotate.

In a fourth aspect, the invention provides a specific form of the separation. In some preferred forms, the separation may be in the form of a channel, for example a channel which may be sealed in which the second chamber may be plugged. In some preferred embodiments, the channel is located at the bottom of the first chamber (i.e. the channel and the mouth of the first chamber for collecting the liquid sample are located at two ends of the first chamber), so that when the liquid sample is collected, the mouth is usually facing upwards, and the channel is facing downwards, which facilitates the liquid sample to enter the first chamber and enter the second chamber directly due to the natural action of gravity. In some preferred forms, the bottom of the first chamber may be provided with a slope which is slightly inclined towards the channel, and the liquid sample may flow along the slope towards the channel, and the angle of the slight inclination may be within 5 °.

In some preferred forms the second chamber is mounted in the channel prior to use of the product, although this mounting must be removable, for example by insertion of the second chamber into the channel with an outer wall that closely matches an inner wall of the channel. In some preferred forms, the tight fit between the second chamber and the passageway must be a band seal, either from the material of the second chamber after assembly or by the addition of a sealing member to the mating surface.

In some preferred forms, the channel has an opening communicating with the first chamber and the second chamber also has an opening for collecting the sample, and after the second chamber is fitted in the channel, the opening in the second chamber must coincide with the opening in the channel so that the liquid sample can be collected in the second chamber. In some preferred forms, the opening in the second cavity is flush with the surface of the opening of the channel. In other preferred forms, the surface of the opening of the second cavity is slightly higher than the surface of the opening of the channel. In other preferred forms, the opening of the second cavity and the opening surface of the channel form a smooth transition and a lower face sloping downwards from the inside to the outside. Regarding the design and cooperation of the structure at the opening, the main function is to avoid the liquid sample from accumulating at the gap opening between the second chamber and the channel as far as possible, because such accumulation easily causes the leakage of the liquid sample during the separation process of the second chamber and the channel, and through the structural high-low layering, the liquid sample can flow to other positions at the bottom of the first chamber along the structure, rather than accumulating at the cooperation position of the second chamber and the channel, besides through the sealing between the matching surfaces, the sealing can be strengthened through the structural design at the opening, and any leakage is avoided as far as possible.

In some preferred modes, need carry on spacingly to the cooperation between second chamber and the passageway, otherwise the second chamber may slide into first intracavity through this passageway, and this kind of condition must be stopped, because not only the exposed part of second chamber gets into first intracavity and can cause the pollution to the liquid sample, and moreover, the passageway itself is an opening to first chamber, originally a stopper that the second chamber can regard as the passageway, if the second chamber falls into first intracavity, must cause the leak of passageway department, the liquid sample can leak in a large number. In some preferred modes, the inner wall of the channel and the outer wall of the second cavity are provided with mutually matched limiting structures, the limiting structures limit the installation limit position of the second cavity to a position in the channel, and once the second cavity is installed in place, the second cavity cannot move inwards any more.

As a specific implementation manner of the limiting structure, an outer step surface is arranged on the inner wall of the channel, the outer step surface is used as a boundary, the inner diameter of a part close to the first cavity is larger than that of a part close to the outside, meanwhile, an inner step surface matched with the outer step surface is arranged on the outer wall of the second cavity, the inner step surface is used as a boundary, the inner diameter of a part close to the first cavity is smaller than that of a part close to the outside, and therefore the limit installation position of the second cavity can be limited at the matching point due to the matching of the inner step surface and the outer step surface, and when the second cavity is installed, the step surface is clamped and cannot move inwards further, so that the limit of the second cavity is realized.

The channel is a structure which enables the first cavity and the second cavity to be assembled, the channel can be regarded as a part of the first cavity, and in some preferred modes, after the second cavity is installed, the outline of the second cavity does not exceed the outer surface of the channel. This case makes it possible to make the first chamber and the second chamber integral before use, but in this case, the second chamber cannot be taken out, and therefore, in some preferred modes, when the sealing element is used for sealing the first cavity, the second cavity and the separation part, the second cavity can be gradually ejected outwards, when all sealing processes (including sealing of the first sealing member to the first chamber, sealing of the second sealing member to the second chamber, and sealing of the third sealing member to the separation site) are completed, a portion of the second chamber protrudes outside the outline of the first chamber, and the second chamber can be separated from the first chamber by the protruding portion, the second chamber at the separation site is a chamber whose sealing is completed, the liquid sample therein may be used for a secondary confirmation test, and the second chamber may be transported independently to a secondary testing mechanism after being detached from the first chamber.

In a fifth aspect, the present invention provides a test element disposed in a first chamber, wherein the first chamber has a relatively independent space therein, for example, a detection region is disposed on a side wall of the first chamber and can be isolated from or connected to the first chamber, the test element is disposed in the detection region, and a liquid sample first enters the first chamber and then enters the detection region from the first chamber to react with the test element, thereby performing detection.

The separation of the detection zone from the body chamber of the first chamber is intended to separate a portion of the sample from the test element, thereby ensuring that at least a portion of the liquid sample is not in contact with the test element, and that this portion is not contaminated by the test element and can be collected in the second chamber for secondary detection confirmation.

In some preferred forms, the location where the test element is located is visible.

In a sixth aspect, the present invention provides a blocking element for blocking or opening a communication relationship between a first chamber and a test area, wherein in an initial state of collecting a sample, the blocking element blocks the first chamber from the test area, at this time, a liquid sample cannot enter the test area, and the test element does not contact or react with the liquid sample.

In some preferred forms, the bottom of the first chamber is provided with an inlet communicating with the detection zone, and the blocking member is provided at the inlet. In some preferred modes, the blocking element and the second sealing element are linked, the second sealing element completes the sealing of the second cavity and simultaneously touches the blocking element to open the inlet communicated with the detection area, the sealing of the second cavity is completed at the moment, the liquid sample in the second cavity is ensured not to have any contact with the sample in the detection area, and the accuracy of secondary detection is ensured.

In some preferred modes, the blocking element and the third sealing element are linked, the blocking element is triggered to open the inlet communicated with the detection area while the third sealing element completes the sealing of the separation part, and the second sealing element completes the sealing of the second cavity in the process of sealing the separation part by the third sealing element, so that when the third sealing element completes the sealing of the separation part, the sealing of the second cavity is previously completed, and therefore, the inlet of the detection area is opened at the moment, the liquid sample in the second cavity cannot be in any contact with the sample in the detection area, and the accuracy of secondary detection can be ensured.

In a seventh aspect, the present invention provides a method for collecting a test sample, the method using the sample collecting device as described above, the device comprising a first chamber for collecting a liquid sample, a second chamber for collecting a liquid sample for confirmation of test, the first chamber and the second chamber being capable of being combined or separated; the sample detection device also comprises a second sealing element for sealing the second cavity, and a third sealing element for sealing the separation part of the first cavity and the second cavity when the first cavity and the second cavity are separated.

In some preferred modes, the device further comprises a first sealing element for sealing the first cavity.

In some preferred forms, the first sealing member is capable of sealing the second cavity with the second sealing member during the sealing of the first cavity.

In some preferred forms, the first sealing member is capable of being sealed by the third sealing member at the separation during the sealing of the first chamber.

In some preferred forms, one of the second sealing element and the third sealing element is in communication with the first sealing element and the second sealing element is in communication with the third sealing element; or the second sealing element and the third sealing element are linked with the first sealing element.

In some preferred forms, the sample collection method further includes a first linkage member for linking the first sealing member and the third sealing member.

In some preferred forms, the sample collection method further includes a second linkage member for linking the second sealing member and the third sealing member.

In some preferred forms, the separation site includes a channel that is sealed by a second chamber prior to sample collection.

In some preferred modes, the second cavity can move in the channel, and a limiting structure for preventing the second cavity from sliding into the first cavity is arranged in the channel.

In some preferred forms, the second chamber and the first chamber are capable of relative movement during the sealing process such that a portion of the second chamber is pushed out of the first chamber.

In some preferred modes, a blocking element capable of preventing the liquid sample from flowing into the detection zone is arranged in the first cavity, and the blocking element can open or close an inlet of the first cavity communicated with the detection zone so as to prevent or release the liquid sample from flowing into the detection zone; the third sealing element seals the separation site and simultaneously triggers the blocking element to open the detection zone inlet.

In some preferred embodiments, the method comprises placing the first chamber and the second chamber in fluid communication via a channel, and allowing the liquid sample to flow from the first chamber to the second chamber through the channel or a port of the channel, such that the liquid sample can naturally enter the second chamber while the liquid sample is being loaded into the first chamber. In some preferred forms, the channel is sealed prior to separation of the first and second chambers. In some preferred forms, the channel is sealed during separation of the first and second chambers.

In some preferred forms, the method includes sealing the first and second chambers in which the sample is collected, the sealing being such that the first and second chambers are capable of relative movement such that a portion of the second chamber is pushed from within the first chamber. In some preferred forms, a portion of the second chamber is exposed outside the first chamber during the sealing process. In some preferred forms, the sealing of the first chamber is accompanied by sealing of the second chamber.

In some preferred forms, the method includes sealing a passage connecting the first and second chambers, wherein the first and second chambers are capable of relative movement during the sealing process such that a portion of the second chamber is pushed out of the first chamber. In some preferred forms, a portion of the second chamber is exposed outside the first chamber during the sealing process. In some preferred forms the sealing of the first chamber is accompanied by sealing of the passage. In some preferred forms, the sealing process for the channel follows the sealing process for the second cavity. In some preferred forms, the second chamber is pushed outwardly while the channel is sealed.

In an eighth aspect, the present invention provides a method for detecting the presence of an analyte in a collected liquid sample, the method comprising collecting a sample to be detected by using the above-mentioned collecting device or collecting method, and performing a primary detection on the sample in the first chamber after a certain amount of the sample is collected in the first chamber.

In some preferred embodiments, the second chamber where the sample is collected is first sealed and the sample in the first chamber is then tested by the test element.

In some preferred embodiments, the first chamber and the second chamber are separated first, and then the sample in the first chamber is tested by the test element. Since the liquid sample collected by the second cavity is used for the secondary detection, the second cavity is separated firstly, and the liquid sample in the second cavity for the secondary detection can be prevented from being polluted by the test element in the primary detection.

In some preferred embodiments, the channel connecting the first chamber and the second chamber is first sealed, and then the sample in the first chamber is tested by the test element.

In some preferred embodiments, the test area and the sample collection area are isolated by the blocking element during collection of the sample, and after collection of the sample is completed, the isolation between the test area and the sample collection area by the blocking element is released to allow the sample to enter the test area.

In some preferred embodiments, a detection zone is disposed within the first chamber. In some preferred embodiments, a test element is disposed within the detection region. In some preferred schemes, an inlet which can be communicated or closed is arranged between the detection area and the first cavity. In some preferred aspects, the inlet can cooperate with the blocking member, be blocked by the blocking member to close, or be opened to communicate the detection region with the first chamber.

The following should also be included in the technical solution of the present invention:

the liquid sample detection device comprises a first cavity for collecting a liquid sample, a second cavity for collecting a confirmation detection liquid sample, and a first cavity and a second cavity which can be combined or separated; the sample testing device also comprises a second sealing element for sealing the second cavity and a third sealing element for sealing the separation of the first cavity and the second cavity.

In some preferred aspects, the first chamber and the second chamber can be in fluid communication when the first chamber and the second chamber are joined.

In some preferred schemes, after the second sealing element seals the second cavity, the third sealing element seals the separation position.

In some preferred aspects, the third sealing element completes the sealing of the separation after the second sealing element completes the sealing of the second cavity.

In some preferred aspects, the device further comprises a first sealing element for sealing the first cavity.

In some preferred aspects, the first sealing element is capable of sealing the second cavity with the second sealing element during the sealing of the first cavity.

In some preferred aspects, the first sealing element is capable of being sealed by the third sealing element at the separation during the sealing of the first cavity.

In some preferred aspects, one of the second sealing element and the third sealing element is in communication with the first sealing element and the second sealing element is in communication with the third sealing element.

In some preferred aspects, the second sealing element and the third sealing element are both in communication with the first sealing element.

In some preferred aspects, the sample testing device further comprises a first linkage member for linking the first sealing member and the third sealing member.

In some preferred embodiments, the linkage is synchronous rotation.

In some preferred embodiments, the linkage is a synchronous movement toward the inside of the sample.

In some preferred aspects, the separation site includes a channel within which the second chamber is movable.

In some preferred schemes, a limiting structure of the second cavity is arranged in the channel.

In some preferred embodiments, the second chamber is sealed and movable along the channel in a direction away from the first chamber.

In some preferred embodiments, a detection zone is included where the sample is initially collected, and the detection zone can be turned on or off.

In some preferred embodiments, the detection zone is closed when the second chamber is not collecting a sample or is collecting a sample, and the sample can enter the detection zone when the second chamber is completely collected and sealed.

In some preferred aspects, the device further comprises a blocking element, wherein the blocking element can open or close the detection inlet.

In some preferred schemes, the blocking element can be linked with the third sealing element, and the blocking element can open the detection area inlet under the driving of the third sealing element.

In some preferred schemes, the blocking element can be linked with the second sealing element, and the blocking element can open the detection area inlet under the driving of the second sealing element.

In some preferred schemes, the blocking element can be linked with the first sealing element, and the blocking element can open the detection area inlet under the driving of the first sealing element.

A sample collection method comprises the steps of firstly enabling a sample to enter a cavity, storing the sample for initial detection and the sample for secondary confirmation detection in the cavity, after the sample collection is completed, isolating the sample for secondary confirmation detection independently, and then detecting the sample for initial detection.

A method of collecting a sample, the method providing a sample collection device comprising a first chamber for collecting a liquid sample, a second chamber for collecting a confirmatory test liquid sample, the first and second chambers being capable of being joined or separated; the sample testing device also comprises a second sealing element for sealing the second cavity and a third sealing element for sealing the separation of the first cavity and the second cavity.

In some preferred embodiments, the first chamber is filled with the sample, and the second chamber is filled with the sample.

In some preferred embodiments, the first and second chambers are filled with the sample simultaneously.

In some preferred embodiments, the second chamber is sealed with a second sealing member after the second chamber has been subjected to sample collection.

In some preferred schemes, after the sealing of the second cavity, a third sealing element is used for sealing the separation position.

In some preferred aspects, the second chamber is sealed simultaneously with the sealing of the separation by a third sealing element.

In some preferred embodiments, the first chamber and the second chamber are sealed with a first sealing element after sample collection.

In some preferred aspects, the first chamber is sealed with the first sealing member while the second chamber is sealed with the second sealing member.

In some preferred aspects, the first chamber is sealed with a first sealing element while the separation is sealed with a third sealing element.

In some preferred aspects, the second sealing element and the third sealing element seal in a ganged manner.

In some preferred aspects, the first sealing element and the second sealing element seal in unison.

In some preferred aspects, the first sealing element and the third sealing element seal in unison.

In some preferred embodiments, the linkage is synchronous rotation.

In some preferred embodiments, the second chamber is sealed and the channel is moved away from the first chamber.

A method for detecting a sample, which collects the sample by the method, comprises a detection area, and the detection area can be opened or closed.

In some preferred embodiments, the second chamber closes the detection zone when no sample is collected or when a sample is collected, and opens the detection zone when collection of the second chamber is complete and sealed, allowing the sample to enter and be detected.

The invention has the beneficial effects that: by adopting the structure, the device has the characteristics of simple and reasonable structure, low cost of used materials and excellent performance; the secondary detection is convenient. Particularly, when subsequent confirmation detection is required, the whole detection device is not required to be sent to the testing mechanism for detection, but only the second cavity is taken out of the device and then sent to the detection structure, so that the detection device is safe, saves space and cost, and is more environment-friendly.

Drawings

Fig. 1 is an overall structural outline view of the present invention.

Fig. 2 is a cross-sectional view of the overall structure of the present invention after sealing is completed.

Fig. 3 is an exploded view of the overall structure of the present invention.

Fig. 4 is a cross-sectional exploded view of the overall structure of the present invention.

Fig. 5 is an overall configuration outline of the second chamber after being sealed.

Fig. 6 is a sectional view of the entire structure after the second chamber is sealed.

Fig. 7 is a sectional view of the entire structure before sealing.

Fig. 8 is a cross-sectional view of the overall structure at a first stage of sealing.

Fig. 9 is a perspective view of the first chamber.

Fig. 10 is a cross-sectional view of the first chamber.

Fig. 11 is a structural view of the first seal member.

Fig. 12 is a schematic view of a first linkage element.

Fig. 13 is a cross-sectional view of the invention with the second chamber hidden showing the position of the gasket.

FIG. 14 is another implementation of a sealing element.

Reference numbers in the figures: the first chamber 1, the second chamber 2, the detection region 3, the sample inlet 4, the channel 5, the first collection port 6, the second collection port 7, the outer step surface 8, the inner step surface 9, the first sealing member 10, the second sealing member 11, the first sealing portion 12, the second sealing portion 13, the shoulder 14, the opening 15 of the channel 5, the third sealing member 16, the first thread structure 17, the second thread structure 18, the third thread structure 19, the first linkage member 20, the second linkage member 21, the inner cap surface 22, the inner shaft hole 23, the outer cap surface 24, the linkage hole 25, the linkage pin 26, the upper cap surface 27 of the second sealing member, the lower cap surface 28 of the third sealing member, the inner step surface 29, the slit 30, the blocking member 31, the inlet 32 of the detection region 3, the stopper 33, the protrusion 34, the stopper ring 35, the outer step surface 36, and the sealing retainer ring 37.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings, and it should be noted that the embodiments are merely specific descriptions of the present invention and should not be construed as limiting the present invention.

The present invention is further described with reference to the structures or terms of art used, which are understood and interpreted according to the ordinary skill in the art unless otherwise indicated.

Detection of

Detection refers to assaying or testing for the presence of a substance or material, such as, but not limited to, a chemical, organic compound, inorganic compound, metabolic product, drug or drug metabolite, organic tissue or a metabolite of organic tissue, nucleic acid, protein, or polymer. In addition, detection indicates the amount of the test substance or material. Further, the assay means immunodetection, chemical detection, enzyme detection, and the like.

Sample(s)

The sample that can be detected by the detection device of the present invention includes a biological fluid (e.g., a case fluid or a clinical sample). The liquid sample or liquid sample may be derived from solid or semi-solid samples, including fecal matter, biological tissue, and food samples. The solid or semi-solid sample may be converted to a liquid sample by any suitable method, such as mixing, triturating, macerating, incubating, dissolving, or enzymatically digesting a solid sample in a suitable solution (e.g., water, phosphate solution, or other buffered solution). "biological samples" include samples derived from animals, plants and food, including, for example, urine, saliva, blood and components thereof, spinal fluid, vaginal secretions, sperm, feces, sweat, secretions, tissues, organs, tumors, cultures, cell cultures and media of tissues and organs derived from humans or animals. Preferably the biological sample is urine. Food samples include food processing materials, end products, meat, cheese, wine, milk and drinking water. Plant samples include those derived from any plant, plant tissue, plant cell culture and medium. An "environmental sample" is derived from the environment (e.g., a liquid sample from a lake or other body of water, a sewage sample, a soil sample, groundwater, seawater, and a waste liquid sample). Environmental samples may also include sewage or other wastewater.

Any analyte can be detected using the present invention and a suitable detection element. The invention is preferably used for detecting drug small molecules in saliva and urine. Of course, the collecting device of the present invention can collect any form of the above samples, whether the sample is initially solid or liquid, as long as the liquid or liquid sample flows into the first chamber, the liquid sample can flow into the second chamber at the same time or later, because the second chamber can be combined with or separated from the first chamber, when the sample is initially collected, the first chamber and the second chamber are combined, the user can complete the collection of the liquid sample in the first chamber and the second chamber by one collecting action, when the subsequent confirmation detection is needed, the second chamber is separated from the first chamber, so that the liquid in the first chamber can be primarily detected, and the second chamber can be secondarily detected. Optionally, the functional status of the first chamber and the second chamber may be called, that is, the liquid in the second chamber may be detected for the first time, and the liquid in the first chamber may be detected for the second time.

Test element

The test element may be a lateral flow test strip which detects a plurality of analytes. Of course, other suitable test elements can be used in the present invention, and any element that can detect whether a sample or specimen contains an analyte of interest can be referred to as a test element, and such detection can be based on any of the technical principles, immunology, chemistry, electricity, optics, physics, and the like.

Various test elements may be combined for use in the present invention. One form is a test strip. Test strips for the analysis of analytes, such as drugs or metabolites indicative of a physical condition, in a sample may be in various forms, such as immunoassay or chemical assay forms. The test strip may be used in a non-competitive or competitive assay format. The test strip includes a bibulous material having a sample application area, a reagent area, and a test area. The sample is added to the sample application zone and flows by capillary action to the reagent zone. In the reagent zone, the sample binds to the reagent if the analyte is present. The sample then continues to flow to the detection zone. Other reagents, such as molecules that specifically bind to the analyte, are immobilized at the detection zone. These reagents react with the analyte (if present) in the sample and bind the analyte to the zone, or to one of the reagents of the reagent zone. The label for indicating the detection signal is present in the reagent zone or in a separate label zone.

A typical non-competitive assay format is one in which a signal is generated if the sample contains the analyte and no signal is generated if the analyte is not present. In a competition method, a signal is generated if the analyte is not present in the sample and no signal is generated if the analyte is present.

The test element is a test paper, and can be made of water-absorbing or non-water-absorbing materials. The test strip may include a variety of materials for liquid sample delivery. One of the test strips may be coated with another material, such as a nitrocellulose membrane coated with filter paper. One region of the test strip may be selected from one or more materials and another region may be selected from a different one or more materials. The test strip may be adhered to some support or hard surface for improved strength when the test strip is held in place.

The analyte is detected by a signal producing system, such as one or more enzymes that specifically react with the analyte, and one or more compositions of the signal producing system are immobilized on the analyte detection zone of the test strip by a method such as that described above for the immobilization of a specific binding substance on the test strip. The signal-producing substance can be on the sample addition zone, reagent zone, or detection zone, or the entire test strip, and the substance can be impregnated on one or more materials of the test strip. A solution containing the signal is applied to the surface of the strip or one or more materials of the strip are immersed in the solution containing the signal. The strip to which the solution containing the signal substance was added was dried.

The various regions of the test strip may be arranged as follows: the device comprises a sample adding area, a reagent area, a detection area, a control area, a sample adulteration area and a liquid sample absorption area. The control zone is located behind the detection zone. All zones may be arranged on a strip of test paper using only one material. It is also possible to use different materials for the different zones. The zones may be in direct contact with the liquid sample, or different zones may be arranged according to the direction of flow of the liquid sample, with the ends of each zone being contiguous with and overlapping the ends of the other zone. The material used can be a material with good water absorption such as filter paper, glass fiber or nitrocellulose membrane. The test strip may take other forms.

A commonly used reagent strip is a nitrocellulose membrane reagent strip, i.e., a detection area comprises a nitrocellulose membrane, and a specific binding molecule is fixed on the nitrocellulose membrane to display the detection result; and may be a cellulose acetate film, a nylon film, or the like. Such as the reagent strips or devices containing the reagent strips described in some of the following patents: US 4857453; US 5073484; US 5119831; US 5185127; US 5275785; US 5416000; US 5504013; US 5602040; US 5622871; US 5654162; US 5656503; US 5686315; US 5766961; US 5770460; US 5916815; US 5976895; US 6248598; US 6140136; US 6187269; US 6187598; US 6228660; US 6235241; US 6306642; US 6352862; US 6372515; US 6379620; and US 6403383. The test strips disclosed in the above patent documents and similar devices with test strips can be applied to the test element or the test device of the present invention for detecting an analyte, such as an analyte in a sample.

The test strip used in the present invention may be a so-called Lateral flow test strip (Lateral flow test strip), and the specific structure and detection principle of these test strips are well known to those skilled in the art. A typical test strip comprises a sample collection area comprising a sample receiving pad, a labeling area comprising a labeling pad, a detection area comprising a bibulous pad, and a bibulous area comprising a chemical reagent, such as an immunological reagent or an enzymatic reagent, necessary to detect the presence of the analyte. A commonly used detection reagent strip is a nitrocellulose membrane reagent strip, that is, a detection area comprises a nitrocellulose membrane, and a specific binding molecule is fixed on the nitrocellulose membrane to display a detection result; it may be a cellulose acetate film, a nylon film, etc., and it may also include a detection result control region downstream of the detection region, and usually, the control region and the detection region are in the form of a transverse line, which is a detection line or a control line. Such test strips are conventional, but other types of test strips that utilize capillary action for testing are also contemplated. In addition, typically, the test strip has a dry chemical reagent component, such as an immobilized antibody or other reagent, which when exposed to a liquid, flows along the test strip by capillary action, and as it flows, the dry reagent component is dissolved in the liquid, and the next zone is processed to react the dry reagent in that zone, thereby performing the necessary test. The liquid flow is mainly by capillary action. These test elements are described and documented in the following documents: lefukang 'research on regeneration treatment of nitrocellulose membranes and protein adsorption capacity thereof'; malanhuang, Liqiang et al analysis of performance of chromatography material in colloidal gold diagnostic kit; wangyong, Wanglauca et al, a novel colloidal gold immunochromatographic test strip. The detection device can be used in the detection device of the present invention, or can be disposed in the detection chamber to contact the liquid sample, or can be used to detect the presence or quantity of an analyte in the liquid sample entering the detection chamber.

In addition to the test element in the form of a test strip, which is itself used to contact the fluid in the first chamber 1 to test whether the fluid sample contains an analyte, in some preferred embodiments, the test element may be disposed on a centralized test card having a plurality of recesses, the test element being disposed in the recess, the entire test card being disposed in the detection zone 3, and the fluid sample entering the first chamber 1 can enter the detection zone 3 through the inlet 4 due to the inlet 4 communicating the first chamber 1 with the detection zone 3, and can be detected by the test element on the test card. Of course, additional carriers besides those disclosed above may also be employed in the present invention as carriers for carrying test strips. For example, in some embodiments, the first chamber may be used to collect a liquid sample and then the sample may be tested by inserting the sample into the first chamber using a separate test strip or a card or carrier with a test strip. It will be appreciated by those skilled in the art that the test strips may not be provided on a carrier, but may be present independently, and that the detection zone 3 of the present invention may be absent in some cases, or the test strips may be absent in some cases, as described in the present invention.

Analyte substance

Examples of analytes that can be used in the present invention include small molecule substances, including drugs of abuse (e.g., drugs of abuse). By "drug of abuse" (DOA) is meant the use of a drug (usually acting to paralyze nerves) at a non-medical destination. Abuse of these drugs can result in physical and mental damage, dependence, addiction and/or death. Examples of drug abuse include cocaine; amphetamine AMP (e.g., black americane, white amphetamine tablets, dextroamphetamine tablets, Beans); methamphetamine MET (crank, methamphetamine, crystal, speed); barbiturate BAR (e.g., Valium, Roche Pharmaceuticals, Nutley, New Jersey); sedatives (i.e., sleep-aid drugs); lysergic acid diethylamide (LSD); inhibitors (downs, goofballs, barbs, blue devils, yellow jacks, hypnones); tricyclic antidepressants (TCAs, i.e., imipramine, amitriptyline and doxepin); dimethyldioxymethylaniline MDMA; phencyclidine (PCP); tetrahydrocannabinol (THC, pot, dope, hash, weed, etc.); opiates (i.e. morphine MOP or, opiates, cocaine COC, heroin, dihydrocodeinone); anxiolytic and sedative hypnotic, anxiolytic is a kind of mainly used for relieving anxiety, stress, fear, stabilize mood, have hypnotic sedative effects at the same time, including benzodiazepine BZO (benzodiazepines), atypical BZ, fuse dinitrogen NB23C, benzodiazepine, BZ receptor ligand, ring-opening BZ, diphenylmethane derivatives, piperazine carboxylate, piperidine carboxylate, quinazolone, thiazine and thiazole derivatives, other heterocycles, imidazole type sedative/analgesic (such as dihydrocodeinone OXY, methadone MTD), propylene glycol derivative-carbamate, aliphatic compound, anthracene derivatives, etc.. The detection device of the invention can also be used for detecting the detection which belongs to the medical application and is easy to take overdose, such as tricyclic antidepressant (imipramine or the like) and acetaminophen. After being absorbed by human body, the medicines are metabolized into small molecular substances, and the small molecular substances exist in body fluids such as blood, urine, saliva, sweat and the like or exist in partial body fluids.

For example, analytes to be detected using the present invention include, but are not limited to, creatinine, bilirubin, nitrite, protein (non-specific), hormones (e.g., human chorionic gonadotropin, progesterone hormone, follicle stimulating hormone, etc.), blood, leukocytes, sugars, heavy metals or toxins, bacterial material (e.g., proteins or carbohydrate materials directed against specific bacteria, such as Escherichia coli 0157: H7, staphylococci, Salmonella, Clostridium, Campylobacter, L.monocytogenes, Vibrio, or Cactus), and substances associated with physiological characteristics in urine samples, such as pH and specific gravity. Any other clinical urine chemistry assay can be tested using a lateral flow assay format in conjunction with the device of the present invention.

Combination and separation of a first chamber and a second chamber

The sample detection device comprises two chambers, namely a first chamber 1 and a second chamber 2, for collecting the liquid sample, wherein the first chamber 1 is used for collecting the liquid sample for initial detection, the second chamber 2 is used for collecting the liquid sample for secondary confirmation detection, and the first chamber 1 and the second chamber 2 can be combined together, so that the liquid sample for initial collection can be simultaneously received, namely, when a user uses the product of the invention, the liquid sample can be simultaneously injected into both the first chamber 1 and the second chamber 2 only by one-time collection operation.

In some embodiments of the present invention, as shown in fig. 2, 7 and 8, the first chamber 1 is provided with a channel 5 at the bottom, one end of the channel 5 is communicated with the inside of the first chamber 1, the other end is communicated with the outside, the second chamber 2 can be assembled into the channel 5, the bottom of the second chamber 2 is sealed to form a plug for the channel 5, as mentioned above, because the sample has certain fluidity, when the user collects the sample, the collection opening is usually upward, for example, as shown in fig. 9 and 10, the first collection opening 6 is upward, the channel 5 (i.e. the opening 7 of the second chamber 2) is located below the first collection opening 6, the user injects the fluid sample from the first collection opening 6, and the sample flows along the inner wall of the first chamber 1 to the second chamber 2 due to gravity, and is collected by the second chamber 2, that is, the second chamber 2 at the bottom is preferentially loaded with the sample, the first chamber 1 will then continue to be filled until the amount required for one test is reached.

Since the liquid sample in the first chamber 1 is directly used for detection and the liquid sample in the second chamber 2 may be transported after being sealed and enter the confirmation detection mechanism for secondary confirmation detection, therefore, the liquid sample in the first cavity 1 is initially detected to become a non-original sample, and then the sample in the first cavity 1 is detected, which has an influence on the accuracy of the secondary confirmation test result, it is necessary to separate the first chamber 1 from the second chamber 2, moreover, it is required to satisfy the requirement that the first chamber 1 and the second chamber 2 can independently form a sealed chamber after being separated, and the liquid samples in the chambers are packaged in the sealed chamber, and simultaneously, the sample can not be sealed from the separation part during the separation process of the first cavity 1 and the second cavity 2, this requires a structure of the separation and a sealing structure of each of the first and second chambers.

In some preferred modes, the second cavity 2 collects the sample through the first cavity 1, the second cavity 2 can be detachably connected or combined with the first cavity 1, the second cavity 2 is combined with the first cavity 1 in the sample collection process, and the second cavity 2 can be pushed out of the first cavity after the sample collection is finished.

In some preferred forms, the second chamber 2 comprises a second collection port for collecting the sample, the second collection port being capable of being in fluid communication with the interior of the first chamber when the second chamber collects the sample. In some preferred forms the second chamber is disposed at the bottom of the first chamber. In some preferred forms, the first chamber 1 has a first collection port 37 for collecting a sample. In some preferred modes, the opening directions of the first collecting port 37 and the second collecting port 38 are the same, so that the collecting efficiency of the second chamber 2 can be improved to the maximum extent by the structure, if the directions of the first collecting port 37 and the second collecting port 38 are different, when a sample enters the second chamber through the first chamber, the sample is not smooth as the direction of the collecting port is the same, retention on the surface or the side wall is easy to cause, and because the directions are different, a corner is inevitably generated, the sample is likely to be silted at the corner, collection is not facilitated, the possibility of leakage is increased when the excessive sample at the silting part is deposited, and in addition, if the directions of the collecting ports are different, linkage during sealing is likely to be not smooth.

At the separation site

To this end, the present invention further provides a separation of the first chamber 1 and the second chamber 2, as shown in fig. 7, which is embodied in the form of a channel 5, and which is embodied as a junction of the first chamber 1 and the second chamber 2 when the first chamber 1 and the second chamber 2 are combined, and the second chamber 2 is assembled with the first chamber 1 at an initial stage when the sample collection device is not used, and in this case, as mentioned above, the second chamber 2 serves as a stopper of the channel 5 to prevent the sample from leaking out of the channel 5 at the time of initial loading, and after the sample loading is completed, the second chamber 2 must be able to be withdrawn from the channel 5 without taking out excess sample, which has a high requirement for the assembly and sealing between the second chamber 2 and the channel 5, and the liquid sample should flow as far as possible into the interior of the second chamber 2 and the interior of the first chamber 1 outside the channel 5, rather than pooling at the assembly of the second chamber 2 and the channel 5.

For this purpose, the following forms are designed:

firstly, it is possible to adopt a way in which the mouth 7 of the second chamber 2 (i.e. the opening of the second chamber 2 facing into the first chamber 1) and the surface of the channel 5 facing the opening 15 in the first chamber must be at least flush, the flush face being relatively less prone to the formation of stagnation points, in particular to avoid that the surface of the opening of the channel 5 is lower than the surface of the mouth of the second chamber 2, in which case, inevitably, the liquid sample will enter the gap between the channel 5 and the mouth of the second chamber 2 and will therefore flow out when the second chamber 2 is withdrawn. Furthermore, it should be pointed out that, since the second chamber 2 requires a second sealing portion 13 with the second sealing element 11, this second sealing portion 13 may extend beyond its mouth 7, forming a shoulder 14 between the second sealing portion 13 and the mouth 7, as shown in fig. 3 and 6, it is necessary to ensure that the shoulder 14 is flush with the surface of the channel 5 facing the opening 15 in the first chamber.

This form may then also be: the second collection well 7 is slightly above the opening 15 of the channel 5 or the shoulder 14 of the second chamber is slightly above the opening of the channel 5, so that the liquid sample that is not loaded by the second chamber 2 will generally follow this high-to-low hierarchy to flow into the first chamber 1 outside the channel 5.

Alternatively, the second collection opening 7 may form a slope with a gradual downward slope from inside to outside with the surface of the opening 15 of the channel 5, or the shoulder 14 of the second chamber may form a slope with a gradual downward slope from inside to outside with the surface of the opening 15 of the channel 5, so that the slope may be more favorable for the liquid sample that is not filled in by the second chamber 2 to remain there along into the first chamber 1.

The separation is embodied in the form of a channel 5, which channel 5 is an element open at both ends, one end communicating with the outside and one end communicating with the inside of the first chamber 1, in which the second chamber 2 can be fitted, and in some cases it is possible for the second chamber 2 to follow the channel 5 into the first chamber 1, which must be absolutely avoided. On the one hand, the leakage part of the second chamber 2 may contaminate the liquid sample in the first chamber 1, and on the second hand, if the second chamber 2 is not plugged or pulled out, the channel 5 becomes a leak of the first chamber, and the liquid sample may leak from the first chamber in a large amount, so that it is necessary to provide a limiting structure for the inward movement of the second chamber 2.

As shown in fig. 2, 7, 8 or 9, an outer step surface 8 is provided in the channel 5, and when the outer step surface 8 is taken as a boundary, the inner diameter of the part of the channel 5 in the direction of the outer step surface 8 close to the first cavity 1 is smaller than the inner diameter of the part of the outer step surface 8 in the direction of the outer part, so that a structure with a small inner part and a large outer part is formed on the inner wall of the channel 5, and the outer step surface 8 is just a large boundary, that is, the outer step surface 8 can be taken as a limit structure.

In order to better adapt to the limiting structure, an inner step surface 9 may be further provided on the outer wall of the second chamber 2, and when the inner step surface 9 is taken as a boundary, the inner diameter of the portion of the outer wall of the second chamber in the direction of the inner step surface 9 close to the inside of the first chamber 1 is smaller than the inner diameter of the portion of the outer wall of the second chamber in the direction of the inner step surface 8 close to the outside, that is, the inner step surface 9 may form a fit with the outer step surface 8 to limit the inward movement of the second chamber 2, and in the initial state, the second chamber 2 is assembled in the first chamber 1, and at this time, the inner step surface 8 and the outer step surface 9 are usually in contact fit, and when the inner step surface 8 and the outer step surface 9 are in contact fit, the bottom of the second chamber 2 is flush with the outward mouth surface of the passage 5. Then as the seal progresses, the inner and outer step surfaces 8 and 9 will separate and the second chamber 2 will be gradually withdrawn outwardly.

The above is just one implementation form of the limiting structure, and it should be understood that the limiting structure is a conventional mechanical structure that can be expanded and applied, and is classified as a structure that can prevent the second chamber 2 from moving inwards without limitation, such as a snap ring, an elastic member, etc., which are not listed herein.

In some preferred modes, the assembly surfaces of the second chamber and the channel may further be provided with a sealing structure, where the assembly surfaces refer to an outer surface of the second chamber and an inner surface of the channel, the sealing structure may be in the form of a sealing collar 37, and the sealing collar 37 may limit the assembly of the second chamber and the channel, that is, may perform the same function as the aforementioned limiting structure, and may also perform a sealing function on the separation of the second chamber and the channel. In some preferred modes, the sealing retainer ring is an O-shaped ring. In some preferred embodiments, the sealing collar 37 is a brush structure that scrapes the surface of the second chamber when the second chamber exits the channel, scraping off the sample that may adhere to the second chamber, although an O-ring may also function as described above.

In some preferred forms, the sealing structure and the limiting structure may not be disposed on the assembling surface of the channel and the second cavity, as long as the above-mentioned functions can be achieved, and the assembling position of the structure is not limited by the scope of the present invention.

Method for collecting specimen

The invention provides a method for collecting the sample, which adopts a sample collecting device with the first cavity 1 and the second cavity 2, wherein the first cavity 1 is used for collecting the liquid sample, the first cavity 1 is provided with a sample inlet 4, the second cavity 2 is used for collecting the liquid sample for confirmation detection, and the detection time and the detection purpose of the first cavity 1 and the second cavity 2 are different, but the detection is performed on the collected sample in the same batch. In order to realize the collection and the fractional detection of the same batch, the first cavity 1 and the second cavity 2 can be combined and separated, the collection of the same batch is carried out during the combination, the separation is carried out after the separation, or the separation is carried out after the detection, and the second cavity 2 independently enters a secondary detection program. The sample testing device further comprises a first sealing member 10 for sealing the first chamber 1, a second sealing member 11 for sealing the second chamber 2, and a third sealing member 16 for sealing the separation of the first chamber 1 and the second chamber 2, i.e. the aforementioned channel 5.

In some preferred ways, the first sealing element 10 can seal the second cavity 2 by the second sealing element 11 during the process of sealing the first cavity 1. In some preferred ways, the first sealing element 10, during sealing of the first chamber 1, the passage 5 can be sealed by the third sealing element 16. In some preferred ways, one of the second sealing element 11 and the third sealing element 16 is in communication with the first sealing element 10 and the second sealing element 11 is in communication with the third sealing element 16; or both the second sealing element 11 and the third sealing element 16 are in conjunction with the first sealing element 10. In some preferred forms, the channel 5 is plugged by the second chamber 2 before all the sealing process, in which case the first chamber 1 and the second chamber 2 form a single body which is closed towards the outside and communicates towards the inside. In some preferred ways, the second chamber 2 is moved outwards with respect to the channel 5 during the sealing process.

In some preferred embodiments, the sample collection method includes placing the first chamber 1 and the second chamber 2 in fluid communication via the channel 5, and allowing the liquid sample to flow from the first chamber 1 into the second chamber 2 via the channel 5 or a port (e.g., opening 15) of the channel 5, so that the liquid sample can naturally enter the second chamber 2 while the liquid sample is filled into the first chamber 1. In some preferred ways, the first chamber 1 and the second chamber 2 are separated before the passage 5 is sealed by the third sealing element 16. In some preferred ways, the first chamber 1 and the second chamber 2 are separated during which the channel 5 is sealed by the third sealing element 16.

In some preferred modes, the sample collection method comprises a process of sealing the first cavity 1 and the second cavity 2, wherein the sample is collected, and in the sealing process, the first cavity 1 and the second cavity 2 can move relatively so that a part of the second cavity 2 is pushed out of the first cavity 1. In some preferred ways, a part of the second chamber 2 is exposed outside the first chamber 1 during the sealing process. In some preferred ways, the sealing of the first chamber 1 is accompanied by the sealing of the second chamber 2.

In some preferred modes, the sample collection method comprises a process of sealing the channel 5 connecting the first cavity 1 and the second cavity 2, and in the sealing process, the first cavity 1 and the second cavity 2 can move relatively so that a part of the second cavity 2 is pushed out of the first cavity. In some preferred ways, a part of the second chamber 2 is exposed outside the first chamber 1 during the sealing process. In some preferred ways, the sealing process of the first chamber 1 is accompanied by sealing of the channel 5. In some preferred ways, the sealing process of the channel 5 follows the sealing process of the second chamber 2. In some preferred forms, the second chamber 2 is pushed outwards while sealing the channel 5.

Method for detecting sample

The invention provides a method for detecting a collected sample, which is used for detecting whether an analyzed substance exists in the collected liquid sample, and the detection method comprises the steps of collecting the sample to be detected by adopting the collecting device or the collecting method, and performing primary detection on the sample in the first cavity 1 after a certain amount of the sample is collected in the first cavity 1.

In some preferred embodiments, the second chamber 2 where the sample is collected is first sealed, and then the sample in the first chamber 1 is tested by the test element. In some preferred embodiments, the first chamber 1 and the second chamber 2 are separated first, and then the sample in the first chamber 1 is detected by the test element. Since the liquid sample collected in the second chamber 2 is used for the secondary detection, the second chamber is separated first, and the liquid sample in the second chamber for the secondary detection can be prevented from being contaminated by the test element in the primary detection. In some preferred embodiments, the channel connecting the first chamber 1 and the second chamber 2 is first sealed, and then the sample in the first chamber is detected by the test element. And sealing the channel to prevent the test sample from leaking.

In some preferred modes, a blocking element 31 capable of preventing the liquid sample from flowing into the detection zone is arranged in the first cavity 1, and the blocking element 31 can open or close an inlet 32 of the first cavity 1 communicated with the detection zone, so that the liquid sample is prevented or released from flowing into the detection zone 3; the third sealing element 16 seals the channel 5 and at the same time triggers the blocking element 31 to open the entrance to the detection zone. In some preferred embodiments, the detection zone 3 and the sample collection zone (i.e. the interior of the first chamber) are isolated by the blocking member 31 during the collection of the sample, and after the collection of the sample is completed, the isolation between the detection zone and the sample collection zone by the blocking member 31 is released to allow the sample to enter the detection zone.

In some preferred embodiments, a detection area 3 is provided in the first chamber 1, the detection area generally having an externally visible characteristic, for example, the detection area may be made of a transparent material. In some preferred embodiments, test elements are arranged in the detection region 3. In some preferred embodiments, an inlet 32 is provided between the detection region 3 and the first chamber 1, which can be open or closed. In some preferred solutions, the inlet 32 can cooperate with the blocking element 31, be blocked by the blocking element to close, or be open to communicate the detection zone with the first chamber.

Sealing element

As mentioned above, when the first chamber 1 and the second chamber 2 are combined, only one of the chambers can be sealed, because the two chambers have a common opening and serve as a passage for communicating the two chambers through a passage 5, and after the first chamber 1 and the second chamber 2 are separated, the second chamber 2 needs to be sealed independently, so that the second chamber 2 can seal the liquid sample therein independently, therefore, a sealing device for the second chamber 2 is needed, when the first chamber 1 and the second chamber 2 are separated, the first chamber 1 will inevitably generate a new opening (i.e. an opening 15 outward of the passage 5) which may leak the liquid except for the original opening (which is sealed by the first sealing element 10) due to the withdrawing of the second chamber 2, and at this time, this opening 15 also needs to be sealed to prevent the liquid sample from flowing out, a sealing means for the separation is therefore required.

In view of the above, the present invention provides specific sealing structures of the first chamber 1 and the second chamber 2, which include a first sealing member 10 for sealing the first chamber 1, a second sealing member 11 for sealing the second chamber 2, and a third sealing member 16 for sealing a separation (i.e., the passage 5) of the first chamber 1 and the second chamber 2 when the first chamber 1 and the second chamber 2 are separated.

In some preferred ways, the first sealing element 10 and the mouth 6 of the first chamber 1 are sealed by a first thread structure 17, the second sealing element 11 and the mouth 7 of the second chamber 2 are sealed by a second thread structure 18, and the third sealing element 16 and the separation (i.e. the passage 5) are sealed by a third thread structure 19.

As shown in fig. 7, the whole device is in a state before sealing, at this time, the first sealing element 10, the second sealing element 11 and the third sealing element 16 do not start to seal, when a user collects a proper amount of liquid sample from the mouth part 6 of the first cavity 1, the device can be sealed, the first sealing element 10 is covered on the mouth part 6 of the first cavity 1, the first sealing element 10 is rotatably covered on the mouth part 6 of the first cavity 1, so as to seal the first cavity 1, the second sealing element 11 seals the mouth part 7 of the second cavity 2 in the process that the first sealing element 10 seals the first cavity 1, or the third sealing element 16 seals the channel 5 in the process that the first sealing element 10 seals the first cavity 1, or the second sealing element 11 and the third sealing element 16 respectively seal the second cavity 2 and the channel 5 in the process that the first sealing element 10 seals the first cavity 1, when the first sealing element 10 completes the sealing of the first cavity 1, the second sealing element 11 and the third sealing element 16 both complete the sealing of the second cavity 2 and the channel 5, respectively, or, in the process of the first sealing element 10 sealing the first cavity 1, the second sealing element 11 seals the second cavity 2 with the rotation of the first sealing element 10, and at the same time, the third sealing element 16 also rotates to seal the channel 5, the sealing is completed in the sequence that the second sealing element 11 completes the sealing of the second cavity 2 first, at this moment, the second cavity 2 is assembled, and a state that the second sealing element 11 is still in the first cavity 1 but is actually separated into an independent cavity in a state that the second sealing element 11 is separated from the first cavity 1 at any moment, and then the third sealing element 11 completes the sealing of the channel 5 next time, the first sealing element 1 continues to rotate until the sealing of the first chamber 1 is completed.

As can be seen from the above description, the sealing process of the second sealing element 11 to the second cavity 2 must be performed during the sealing process of the first sealing element 10 to the first cavity 1, and the sealing process of the third sealing element 16 to the channel 5 must also be performed during the sealing process of the first sealing element 10 to the first cavity 1, and the sealing process of the second sealing element 11 to the second cavity 2 and the sealing process of the third sealing element 16 to the channel 5 may be performed sequentially, may be partially overlapped, or may be completely synchronized.

Fig. 8 shows an intermediate stage of the sealing, in the state shown in fig. 8, in which the second sealing element 11 has completed the sealing of the second chamber 2, in which the third sealing element 16 is about to start the sealing of the passage 5, and the first sealing element 1 is in the process of sealing the first chamber 1, that is, in terms of the length of the threads, the length of the first thread formation 17 is equal to the sum of the lengths of the second thread formation 18 and the third thread formation 19, so that the first sealing element 10 is in the process of sealing the first chamber 1,

it should be noted that, during the sealing process, since the first sealing element 1, the second sealing element 2 and the third sealing element 3 are all sealed by screwing, during the screwing process, these sealing elements must have a height reduction, which, in terms of linkage, pushes the second chamber 2 out in a direction away from the first chamber 1, so that a part of the second chamber 2 is exposed outside the first chamber 1, thereby facilitating the removal of the second chamber 2 from the first chamber 1, which will be described in detail in the following linkage element.

In some preferred forms, the sealing element may also be in the form of a plug to effect sealing of the respective cavity. For example, the first sealing element 1, the second sealing element 2 and the third sealing element 3 may all take the form of sealing plugs.

In some preferred forms, a sealing collar may be provided on the inner wall of the passage or the outer wall of the second chamber. In some preferred modes, the sealing and blocking ring can adopt a flexible structure or a brush structure, and the sealing and blocking ring has the function of preventing the second cavity or separated samples from seeping out when the second cavity is withdrawn from the channel.

In some preferred ways, the sealing element need only comprise a second sealing element 11 for sealing the second cavity 2 and a third sealing element 16 for sealing the channel 5, which can be interlocked to seal the second cavity and the channel, respectively.

In some preferred forms, the second sealing element 11 seals the second chamber 2 first, and then the third sealing element 16 seals the channel 5. In some preferred ways, the third sealing element 16 is able to move the second chamber 2 in a direction separating from the first chamber 1 while sealing the channel 5. In some preferred forms, the second chamber 2 need only be partially withdrawn from the first chamber 1.

In some preferred ways, the second sealing element 11 is combined with the second cavity 2 during the sealing of the second cavity 2. In some preferred forms, the third sealing element 16 engages the channel 5 during the sealing of the channel 5, while pressing the second chamber outwards, where the engagement means that the sealing element and the sealed part are closer together or, in the case of already assembled, more tightly assembled, which assembly may further shorten the length or height of the entire assembly, e.g. a screw thread, which will be shorter as the screw thread is screwed in.

In some preferred embodiments, the second sealing element 11 can be detachably combined with or connected to the mouth of the second chamber 2, wherein the combination is the same as the combination above, and the second collection port is a collection port for collecting a sample of the second chamber, and generally speaking, the collection port should be the only opening of the second chamber, and when the opening is closed, the second chamber is in a sealed state.

In some preferred forms, the second sealing element 11 is a second cap removably coupled or connected to the second collection port by a second threaded structure. In some preferred modes, the thread can be arranged on the outer wall of the second cavity part and the inner wall of the second cover body. In some preferred modes, the thread can be arranged on the outer wall of the second cover body and the inner wall of the second cavity part. The internal and external threads can be selected according to the actual requirements, and the invention is not limited to the one shown in the figures. In some preferred modes, the second sealing element is a second plug body, the second plug body can be tightly matched with the inner wall of the opening part of the second cavity, the opening part is inwards squeezed and plugged in the plug mode, the sealing effect can be achieved, and the plug can achieve linkage between the plug bodies in the plugging process. For the purposes of the present invention, the sealing of the sealing element and the linkage of the sealing process are the solutions to be protected by the present invention.

In some preferred forms, the third sealing element is capable of being removably coupled or connected to the mouth of the passageway. In some preferred forms, the third sealing element is a third cap removably coupled or connected to the mouth of the passageway by a third thread formation. In some preferred forms, the third sealing element is a third plug body that is capable of interference fit with the inner wall of the passage mouth. Similarly, the connection or combination of the third sealing element and the channel mouth can also adopt a similar structure as in the previous paragraph, and the invention is not limited to the one shown in the figures, and in some cases, different connection modes can be selected for different cover bodies according to requirements, that is, a combined connection mode is also within the protection scope of the invention.

Linkage element

In order to realize the sealing sequence of the first cavity 1, the second cavity 2 and the channel 5, a linkage structure is required to be arranged for the first sealing element, the second sealing element and the third sealing element, the linkage structure has the function that when the first sealing element seals the first cavity, the second sealing element can also seal the second cavity, and possibly, when the first sealing element completes the sealing, the second sealing element completes the sealing, or before the first sealing element completes the sealing, the second sealing element completes the sealing.

These linkages comprise a first linkage element 20 for linking the first sealing element 10 and the third sealing element 16, and a second linkage element 21 for linking the second sealing element 11 and the third sealing element or the second sealing element 11 and the first sealing element 10.

The first linkage element 20 is a linkage rod, and two ends of the linkage rod are respectively connected with the first sealing element 10 and the third sealing element 16, so that when one of the first sealing element 10 and the third sealing element 16 rotates, the other one can be driven to rotate along with the linkage rod, the rotation along with the linkage rod can be synchronous or discontinuous, but the rotation along with the linkage rod is usually in the same direction, but the possibility of the opposite direction is not eliminated.

As a specific implementation form of the linkage rod, the first sealing element 10 is a cup cover, and has a relatively flat inner cover surface 22, an inner shaft hole 23 is formed in the inner cover surface 22, and the upper end of the linkage rod is inserted into the inner shaft hole 23, as shown in fig. 2, 7, and 8, which is a part of the first sealing element 10. In some cases, the third sealing element 16 is also a cover structure, and has a relatively flat outer cover surface 24, an outer shaft hole is formed in the outer cover surface 24, and the lower end of the linkage rod is inserted into the outer shaft hole, so that the linkage rod can link the first sealing element 10 and the third sealing element 16 through the inner shaft hole and the outer shaft hole, so that the first sealing element 10 and the third sealing element 16 can synchronously rotate, and thus, when the third sealing element 16 is used, the user only needs to rotate the outermost first sealing element 10, and the third sealing element 16 can be synchronously driven to seal the channel 5.

Or, the third sealing element 16 may be as shown in fig. 2, 7 and 8, the lower end of the linkage rod is provided with a linkage hole 25, the outer cover surface 24 of the third sealing element 16 is provided with a linkage pin 26, and the linkage pin 26 is inserted into the linkage hole 25 to realize linkage of the third sealing element 16 and the linkage rod, in this way, the purpose of synchronously rotating the first sealing element 1 and the third sealing element 16 can be achieved, or the lower end of the linkage rod is directly connected to the outer cover surface 24 of the third sealing element 16, in this case, the detachable separation and combination of the first linkage element 20 and the third sealing element 16 cannot be realized, but the function of the device for collecting samples is not affected.

Whichever of the above implementations, the linkage rod connects the first sealing element 10 and the third sealing element 16 through the first chamber 1. And, the linkage rod or the portion used for connection on it needs to have certain shape, usually this shape is not a perfect circle, can be for example square, semicircle or triangle-shaped etc. correspondingly, the inner and outer axle hole on the first sealing element 10 and the third sealing element 16 are the same shape too, then, carry on the peripheral spacing through this shape between linkage rod and the inner and outer axle hole, can not take place relative rotation, the linkage rod can drive the first sealing element and the third sealing element through two axle holes and rotate like this, thus realize the linkage. In other possible modes, the linkage rod and the inner and outer shaft holes can also be round holes which are tightly matched, and the linkage force comes from the friction force between the linkage rod and the abutting surfaces of the inner and outer shaft holes. In other possible modes, the linkage rod and the shaft hole can also be in a stepping linkage fit relation, and under the stepping linkage fit relation, the linkage rod or the inner shaft hole and the outer shaft hole can independently rotate for a certain angle and then synchronously rotate.

Accordingly, in the manner of fitting the linking hole 25 and the linking pin 26, the linking hole 25 and the linking pin 26 may be formed in a shape similar to that described above, or may be formed by using a similar principle.

As another realization form of linkage matching, a shaft hole is arranged on the linkage rod, a matching element which is linked with the inner shaft hole and the outer shaft hole is arranged on the first sealing element or the third sealing element, the matching element and the shaft hole have the same shape, and the shape deviates from the axis of the linkage rod or is non-circular, so that the linkage rod and the matching element can realize linkage. In some preferred modes, the linkage rod can also be matched with the matching element in a stepping type linkage mode.

The second linkage element 21 may directly link the first sealing element 10 and the second sealing element 11, or may link the second sealing element 11 and the third sealing element 16, and the link is not a specific implementation scheme to be limited by the present invention, and the present invention aims to realize the sealing priority order of the three elements. As shown in fig. 2, 7 and 8, the second coupling element 21 is a short piece of coupling pin or the like, the second coupling element 21 is attached to the upper face 27 of the second sealing element, but may also be attached to the lower face 28 of the third sealing element 16 (this is not shown in the figures), and whether the second coupling element 21 is attached to the second sealing element 11 or the third sealing element 16, the other of the second sealing element 11 and the third sealing element 16 (i.e. the one not attached to the second coupling element 21) is provided with a hole for engaging with the second coupling element 21, and similarly, the second coupling element 21 and the hole for engaging with it have a shape which, like the aforementioned coupling rod, may be offset from the center of the second sealing element or the third sealing element, or may be non-circular, such as a square, the purpose of the linkage pin and/or the linkage hole is to drive one of the linkage pin and/or the linkage hole to rotate along with the other linkage pin and/or the linkage hole, the rotation can be synchronous or discontinuous, and the rotation can be in the same direction or in the opposite direction.

When the second sealing element 11 is linked with the first sealing element 10, along with the rotation of the first sealing element 10, the second sealing element 11 can seal the second cavity 2 through the second thread structure 18, and when the second sealing element 11 is linked with the third sealing element 16, since the third sealing element 16 is linked with the first sealing element 10, the second sealing element 11 is also driven to seal the second cavity 2. Whether the direct linkage part of the second sealing element 11 is the first sealing element 10 or the third sealing element 16 can be selected as required in consideration of the structural arrangement of the actual product.

In some preferred forms, the linkage between the second sealing element and the third sealing element may be by a fixed connection. In some preferred manners, the linkage between the first sealing element and the third sealing element may be implemented by a fixed connection, that is, as shown in fig. 13, the second sealing element 11 and the third sealing element 16 may be a fixed connection manner, which is equivalent to that the second sealing element 11 and the third sealing element 16 are connected into a whole, and the linkage can be implemented naturally, and the fixed connection may be implemented by itself, for example, a certain surface of the two sealing elements is fixedly connected or stuck together, or by another component, for example, a connecting rod. Similarly, the linkage between the first sealing element and the second sealing element, and between the first sealing element and the third sealing element can also be naturally realized in this way.

In some preferred forms, the first linkage element is a linkage rod. In some preferred forms, the second linkage element is a linkage pin. In some preferred modes, the linkage refers to synchronous rotation. In some preferred modes, the linkage refers to synchronous movement towards the direction close to the inside of the sample. In the invention, the linkage particularly means that the sealing process is synchronous, for example, synchronous rotation is particularly suitable for the connection mode of a thread structure, and for example, synchronous loading is particularly suitable for the connection mode of a plug, and similar linkage structures can also be adopted in connection modes such as clamping connection, knob connection and the like.

Limiting structure

As mentioned above, the channel 5 is an implementation form of a separation part, and functions to allow the second chamber 2 to be inserted and installed therein and to seal the second chamber, so as mentioned above, it is necessary to limit the fit between the second chamber 2 and the channel 5, otherwise the second chamber 2 may slide into the first chamber 1 through the channel 5, and contaminate the liquid sample or cause a large amount of leakage of the liquid sample at the channel 5. Therefore, the inner wall of the channel 5 and the outer wall of the second chamber 2 are provided with mutually matched limit structures, the limit structures limit the installation limit position of the second chamber 2 to a position in the channel 5, and once the second chamber 2 is installed in place, the second chamber cannot move inwards any more.

As shown in fig. 2, 7 and 8, an outer step surface 36 is provided on the inner wall of the passage 5, the inner diameter of the portion of the passage 5 close to the first chamber 1 is larger than that of the portion close to the outside with the outer step surface 36 as a boundary, and at the same time, as shown in fig. 5-6, an inner step surface 29 matched with the outer step surface 36 is provided on the outer wall of the second chamber 2, and the inner step surface 29 is used as a boundary, and the inner diameter of the portion of the second chamber 2 close to the first chamber 1 is smaller than that of the portion close to the outside, so that the inner step surface 29 and the outer step surface 36 are matched to limit the limit installation position of the second chamber 2 at the matching point, and when the second chamber 2 is installed, the step surface is clamped and cannot move further inwards, so that the limit of the second chamber 2 is realized.

The channel 5 is a structure that enables the first chamber 1 and the second chamber 2 to be assembled, the channel 5 can be regarded as a part of the first chamber 1, and ideally, after the second chamber 2 is installed, the outline (for example, the bottom surface) of the second chamber 2 does not exceed the outer surface of the channel 5, so that the first chamber 1 and the second chamber 2 can be formed into a whole before use, but if the second chamber 2 is difficult to be taken out from the first chamber 1 when the second chamber 2 is used, therefore, the linkage structure of the present invention can also realize that when the sealing elements are used for sealing the first chamber 1, the second chamber 2 and the channel 5, the second chamber 2 can be gradually pushed out, when all the sealing processes (including the sealing of the first sealing element 10 to the first chamber 1, the sealing of the second sealing element 11 to the second chamber 2 and the sealing of the third sealing element 16 to the channel 5) are completed, a portion of the second chamber 2 protrudes from the outer contour of the first chamber 1 (as shown in fig. 2), and the second chamber 2 can be separated from the first chamber 1 by the protruding portion, and the second chamber 2 sealed by the second sealing member 11 is a sealed chamber in which the liquid sample can be used for the secondary confirmation detection, and the second chamber 2 can be independently transported to the secondary detection mechanism after being separated from the first chamber 1.

In order to achieve that the second chamber 2 is gradually pushed out with the sealing process, the length of the thread structure and the length of the linkage element need to satisfy certain requirements, for example, as mentioned above, in the process that the second chamber 2 is first sealed by the second sealing element 11, then the channel 5 is sealed by the third sealing element 16, and in the process that the two are sealed, the first sealing element 11 is always in the process of sealing the first chamber 1, ideally, the third sealing element 16 just completes the sealing at the same time that the first sealing element 11 completes the sealing, in this case, the length of the first thread structure 17 is equal to the sum of the lengths of the second thread structure 18 and the third thread structure 19, and the length of the linkage rod is equal to: when the first sealing member 1 is turned down until the second sealing member 11 just seals the second chamber 2 completely, the relative position of the second chamber 2 to the channel 5 does not change, and when the third sealing member 16 starts to seal the channel 5, the second chamber 2 starts to be pushed out outwards. That is, in the initial stage of closing, the first sealing element 11 drives the second sealing element and the third sealing element to rotate through the linkage rod, at this time, the third sealing element is in an idle rotation state and is not in contact with other parts, the second sealing element is already started to be matched with the thread structure on the second cavity 2, after the second cavity 2 is completely sealed, the first sealing element 11 continues to rotate, at this time, the first thread structure 17 continues to be closed, the third sealing element and the sealed second cavity are pushed downwards through the linkage rod, and while the second cavity 2 starts to move outwards along the channel 5, the third sealing element starts to seal the channel 5 until the channel 5 is completely sealed, at this time, the first sealing element 11 also just completes the sealing of the first cavity 1.

Since a perfect sealing between the second sealing element 11 and the third sealing element 16 is not possible, there will necessarily be a certain gap 30, this part of the gap may enter the liquid sample when the second sealing member 11 seals the second chamber, ideally, therefore, after the second sealing element 11 has been sealed, the third sealing element 16 still idles for a short distance, forcing out the liquid in the gap, the channel 5 is then sealed again, so that the residual liquid sample in the gap is minimized, leakage of the liquid sample during separation is reduced, in this case, the length of the first thread formation 17 needs to be slightly greater than the sum of the lengths of the second thread formation 18 and the third thread formation 19, in practice, the specific size can be adjusted according to the needs, and the main technical scheme to be realized by the invention is the linkage relation of sealing and the sealing sequence.

Barrier element

In addition to the above-mentioned structure, the initial test of the sample using the apparatus of the present invention is also an aspect of the present invention, and therefore, the present invention is desirable that the initial test can be performed after the sample collection is completed, especially after the liquid sample for the secondary confirmation test in the second chamber 2 is completely collected and sealed, so that the detection sample for the secondary confirmation test is not contaminated by the initial test, and the accuracy of the secondary test is ensured, and for this purpose, the present invention further provides a blocking member 31, which, as mentioned above, functions to temporarily block the test element from the liquid sample collected in the initial detection chamber (i.e. the first chamber 1) and temporarily prevent the liquid sample from entering the detection area 3, and of course, can release the sample, so that the blocking member is a member for blocking or opening the communication between the first chamber 1 and the detection area 3, when the initial state of collecting the sample, barrier element 31 separates first chamber 1 and test area 3, and the unable detection zone 3 that gets into of liquid sample this moment, test element can not take place contact or reaction with the liquid sample, and when collecting to a certain extent or at certain opportunity, barrier element 31 opens the detection entry 32 that first chamber 1 communicates detection zone 3, allows partly liquid sample to get into detection zone 3 from detection entry 31 in to carry out the preliminary examination through test element, obtain a preliminary testing result.

As shown in fig. 2-4, the blocking member 31 may be a rotating piece capable of rotating around the axis of the first chamber 1, a blocking plate 33 may be disposed on the rotating piece, before use and in an initial state of collecting a sample, the blocking plate 33 may block the inlet 32, even if a liquid sample enters the first chamber 1 and the second chamber 2, the liquid sample may not enter the detection area 3 and may not contact with the test element, and then the blocking member 31 may interfere with one of the sealing members at a proper time during the rotation of the sealing members, so as to open the inlet 32, and allow the liquid sample to enter the detection area 3 for initial detection.

For example, the blocking element 31 and the second sealing element are linked, and when the second sealing element completes the sealing of the second chamber, the blocking element is touched to open the inlet communicated with the detection area, so that the second chamber is sealed, the liquid sample in the second chamber is ensured not to contact with the sample in the detection area, and the accuracy of secondary detection is ensured.

Or, as shown in fig. 2-4, the blocking element 31 is sleeved on the periphery of the channel 5 and can rotate around the channel, the third sealing element 16 is provided with a protrusion 34, the protrusion 34 can be lowered to a height interfering with the blocking plate 33 when the third sealing element 16 seals the channel 5, the protrusion 34 interferes with the blocking plate 33 at the same time when the third sealing element 16 completes sealing the channel 5, the blocking plate 33 is pushed to drive the blocking element 31 to rotate, and the inlet 32 communicating with the detection area is opened, because the second sealing element completes sealing the second cavity in the process of sealing the separation position by the third sealing element, when the third sealing element completes sealing the separation position, the sealing of the second cavity is previously completed, and therefore, when the inlet of the detection area is opened, the liquid sample in the second cavity does not have any contact with the sample in the detection area, the accuracy of secondary detection can be guaranteed.

In some preferred forms, the blocking member is adapted to open a detection zone for detecting an initial test sample, the detection zone being closed when no sample is collected or when a sample is collected in the second chamber, and the sample being able to enter the detection zone when the collection of the second chamber is completed and sealed.

In some preferred forms, a second sealing member is included for sealing the second chamber, the second sealing member being capable of opening or closing the detection inlet and opening the detection inlet when the second sealing member completes the sealing of the second chamber. In some preferred modes, the second sealing element is linked with the blocking element, and the blocking element is triggered to open the inlet communicated with the detection area at the same time when the second sealing element completes the sealing of the second cavity.

In some preferred forms, the first chamber is capable of being in fluid communication with or isolated from the detection zone. When the detection inlet is opened, the first cavity is in liquid communication with the detection area, and when the detection inlet is closed, the first cavity is separated from the detection area. In some preferred modes, the first sealing element is linked with the second sealing element, the first sealing element can open or close the detection inlet, and when the second sealing element completes the sealing of the second cavity, the first sealing element is driven to open the detection inlet. In some preferred modes, the first sealing element is linked with the blocking element, and the blocking element is triggered by the first sealing element to open the entrance of the communication detection area at the same time when the second sealing element completes the sealing of the second cavity.

In some preferred modes, the third sealing element is linked with the second sealing element, the third sealing element can open or close the detection inlet, and when the second sealing element completes the sealing of the second cavity, the third sealing element is driven to open the detection inlet. In some preferred modes, the third sealing element is linked with the blocking element, and the blocking element is triggered to open the entrance of the communication detection area by the third sealing element at the same time when the second sealing element completes the sealing of the second cavity.

Specific implementation scheme

Before use, the linkage rod and the first sealing element 10, the second sealing element 11 and the third sealing element 16 can be in a separated or partially separated state, at this time, the first sealing element 10 in the separated state can be used for closing the whole device, and the second sealing element 11, the third sealing element 16 and the linkage rod in the separated state can be placed into the first cavity 1 to be used as a complete product package.

In use, the first sealing member 10 is opened and the fittings (including the second sealing member, the third sealing member and the linkage rod) are removed therefrom, and in some cases, the fittings may be individually packaged in other packages.

After the liquid sample is collected, the device is sealed, the related fittings are connected together according to the mode shown in fig. 7, then the first sealing element 10 is rotated to seal the first cavity, in the process, as shown in fig. 8, the linkage rod firstly drives the second sealing element and the third sealing element to move downwards until the second sealing element is contacted with the second collecting port, the second sealing element can seal the second cavity, the whole sealing elements which form a whole moving body through the linkage relation of the linkage elements are moved downwards continuously while the second cavity is sealed, until the second sealing element is sealed, further, the first sealing element continues to rotate downwards, at the moment, the third sealing element starts to seal the channel, and as the second sealing element and the third sealing element or the first sealing element are still in a linkage state, therefore, the second sealing element can drive one side of the sealed second cavity to rotate and move downwards once, at the moment, the downward movement of the second cavity is pushed out relative to the channel, the bottom of the second cavity is gradually exposed out of the bottom of the first cavity until the sealing of the channel is completed, the sealing of the first cavity is completed at the moment, in order to prevent the excessive rotation, a sealing limiting element is further arranged outside the first collecting port, the sealing limiting element is shown in figures 2, 4, 7 or 8 and can be a limiting ring 35, the periphery of the limiting ring is larger than that of the first sealing element, therefore, when the first sealing element rotates to be in contact with the limiting ring, the downward movement cannot be continued, the sealing limiting structure can protect the internal structure, and the damage to the internal structure caused by the excessive rotation is prevented. Of course, the sealing and limiting element may also be a limiting block or a buckle, or the sealing and limiting structure may be realized by the end point of the thread, and when the first sealing element rotates to the end point of the first thread structure, the first sealing element is limited naturally.

Claims

1. A sample detection device is characterized by comprising a first cavity for collecting an initial detection sample, a second cavity for collecting the sample and a detection area for initially collecting the sample,

a channel is arranged at the bottom of the first cavity, one end of the channel is communicated with the interior of the first cavity, and the second cavity is assembled in the channel;

the sealing device comprises a first sealing element for sealing a first cavity, a second sealing element for sealing a second cavity and a third sealing element for sealing a channel of the first cavity and the second cavity, wherein the first sealing element and the opening of the first cavity are sealed through a first thread structure, the second sealing element and the opening of the second cavity are sealed through a second thread structure, and the third sealing element and the channel are sealed through a third thread structure;

the sealing device comprises a first linkage element and a second linkage element, wherein the first linkage element is used for linking the first sealing element and the third sealing element, and the second linkage element is used for linking the second sealing element and the third sealing element or the second sealing element and the first sealing element;

when the second sealing element is in linkage with the first sealing element, the second sealing element can seal the second cavity through the second thread structure along with the rotation of the first sealing element;

comprises a blocking element which can close or open the detection inlet;

the first chamber can be in fluid communication with or separated from the detection zone, the detection zone is closed when the second chamber is not collecting a sample or is collecting a sample, and the sample can enter the detection zone when the second chamber is completely collected and sealed.

2. The apparatus according to claim 1, comprising a detection inlet, wherein the detection inlet can be opened or closed.

3. The apparatus according to claim 2, wherein the detection inlet is opened when the second chamber is completely collected and sealed.

4. The sample testing device of claim 3, wherein the second chamber is capable of being assembled to the bottom of the first chamber, the sample testing device further comprising a second sealing member for sealing the second chamber, the second sealing member being capable of opening or closing the detection inlet and opening the detection inlet when the second sealing member completes the sealing of the second chamber.

5. The sample testing device of claim 4, wherein the second sealing member is operatively associated with the blocking member to actuate the blocking member to open the inlet to the testing area at the same time that the second sealing member seals the second chamber.

6. The specimen-testing device according to claim 4, wherein the test region is provided on a side wall of the first chamber, the first chamber and the test region are in fluid communication when the test inlet is open, and the first chamber and the test region are blocked when the test inlet is closed.

7. The apparatus according to claim 6, wherein the apparatus further comprises a first sealing member for sealing the first chamber, the first sealing member is linked with the second sealing member, the first sealing member can open or close the detection inlet, and the second sealing member drives the first sealing member to open the detection inlet when the second chamber is sealed.

8. The apparatus according to claim 7, wherein the first sealing member is operatively associated with the blocking member, and wherein the blocking member is actuated by the first sealing member to open the access to the detection region while the second sealing member seals the second chamber.

9. The apparatus according to claim 4, further comprising a mounting channel for the second chamber and a third sealing member for sealing the mounting channel, wherein the third sealing member is linked to the second sealing member, and the third sealing member is capable of opening or closing the detection inlet, and when the second sealing member completes the sealing of the second chamber, the third sealing member is driven to open the detection inlet.

10. The sample testing device of claim 9, wherein the third sealing member is operatively associated with the blocking member such that the blocking member is actuated by the third sealing member to open the access to the testing area while the second sealing member seals the second chamber.