CN213232220U - A novel liquefaction post device for blood clot nucleic acid draws - Google Patents
A novel liquefaction post device for blood clot nucleic acid draws Download PDFInfo
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- CN213232220U CN213232220U CN202021882818.8U CN202021882818U CN213232220U CN 213232220 U CN213232220 U CN 213232220U CN 202021882818 U CN202021882818 U CN 202021882818U CN 213232220 U CN213232220 U CN 213232220U
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- nucleic acid
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Abstract
The utility model discloses a novel liquefaction post device for blood clot nucleic acid extraction, which comprises a post body, wherein the top of the post body is provided with a sealing cover, the right side of the top of the post body is provided with a buckle mechanism for controlling the opening and closing of the sealing cover, a combination arm is arranged between the left side of the top of the post body and the left side of the sealing cover, filter paper is placed inside the bottom of the post body, the filter paper is of a two-layer structure, the filter paper is of a porous aggregation structure with the diameter of 30-50 microns, the upper surface of the filter paper is provided with a gasket, a positive funnel-shaped device is integrally formed inside the bottom of the post; this scheme, the closing cap is opened through buckle mechanism to the liquefaction post when using, in adding the column with the blood clot, then installs column and liquid collecting pipe through the screw thread, through high-speed centrifugation, during liquid composition in the blood clot pierces through filter paper and gets into liquid collecting pipe, impurity composition held back in the filter paper top, accomplishes the separation of blood and impurity.
Description
Technical Field
The utility model relates to a biological assay technical field specifically is a novel liquefaction post device for blood clot nucleic acid draws.
Background
The extraction of genomic nucleic acid from blood is a very common requirement, such as the screening of infant genetic diseases (especially blood genetic diseases), paternity testing, and the detection of various diseases of adults, so that the extraction of the content, proportion and genome of various cells in blood is very important for the diagnosis of clinical medical diseases. At present, two methods, namely a column membrane method and a magnetic bead method, are mainly used for extracting genome nucleic acid from blood, and other methods, such as a saturated phenol-chloroform method and a salting-out method, are not widely used, but each nucleic acid extraction method can solve the problem that blood clots are difficult to treat. At present, the filter device in the liquefaction column device mostly adopts a screen (100 meshes) and is relatively expensive, and the double-layer 30-50 micron filter paper is used as a filter barrier in the invention to remove impurity components in blood clots, so that blood cells can pass through the filter smoothly for subsequent nucleic acid extraction and purification experiments.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel liquefaction post device for blood clot nucleic acid draws to solve the problem that proposes in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a novel liquefaction column device for clot nucleic acid extraction comprising:
the cylinder, the top of cylinder is equipped with the closing cap, the control is installed on the top right side of cylinder the buckle mechanism that the closing cap opened and shut, the top left side of cylinder with install the combination arm between the left side of closing cap, filter paper has been placed to the bottom inside of cylinder, filter paper adopts two-layerly, filter paper is the poroid structure of assembling of diameter 30-50 microns, the upper surface of filter paper is equipped with the packing ring, the inside integrated into one piece in bottom of cylinder has just putting hourglass hopper-shaped device, the liquid collecting pipe is installed to the bottom of cylinder.
Through adopting above-mentioned technical scheme, the closing cap is opened through buckle mechanism to the liquefaction post when using, in adding the blood clot into the cylinder, then install cylinder and liquid collecting pipe through the screw thread, through high-speed centrifugation, during liquid composition in the blood clot pierces through filter paper and gets into liquid collecting pipe, impurity composition held back in the filter paper top, accomplishes the separation of blood and impurity.
Preferably, the lower end opening of the column body is contracted into a funnel shape.
By adopting the technical scheme, the surface relaxation degree of the filter paper is ensured, the filter paper is prevented from falling off, and the function of supporting the filter paper is achieved.
Preferably, the buckling mechanism comprises a first vertical elastic sheet, a transverse elastic sheet, a second vertical elastic sheet and an L-shaped elastic sheet, the first vertical elastic sheet is vertically welded at the bottom of the right side of the sealing cover, one end of the transverse elastic sheet is vertically welded at the bottom of the first vertical elastic sheet, the second vertical elastic sheet is vertically welded at the other end of the transverse elastic sheet, the L-shaped elastic sheet is welded at the middle position of the top of the column body, and the second vertical elastic sheet is located on the inner side of the L-shaped elastic sheet.
Through adopting above-mentioned technical scheme, through buckle mechanism, conveniently open and shut the cylinder, add the blood clot in the cylinder.
Preferably, the outer wall of the bottom of the column body is provided with an outer notch, the outer wall of the outer notch is integrally formed with an external thread, the inner wall of the top of the liquid collecting pipe is provided with an inner notch, the inner wall of the inner notch is integrally formed with an internal thread, the liquid collecting pipe is installed at the bottom of the column body through threaded connection, and at the moment, the outer notch and the inner notch are overlapped.
By adopting the technical scheme, the liquid collecting pipe is convenient to mount through threaded connection.
Preferably, the gasket is arranged in a plastic circular ring shape, the inner diameter of the gasket is smaller than the outer diameter of the filter paper, and the gasket is pressed on the two layers of the filter paper.
By adopting the technical scheme, the filter paper is not easy to fall off through the gasket.
Preferably, the right funnel and the gasket clamp the filter paper at the bottom of the column.
Through adopting above-mentioned technical scheme, through just putting hourglass hopper-shaped device and packing ring, filter paper is difficult for down dropping.
Compared with the prior art, the beneficial effects of the utility model are that:
the whole device is simple in structure, easy and convenient to operate, compared with a manual method, time is greatly saved, human interference is small, high-flux operation can be achieved, sensitivity is high, manual operation is few, repeatability is high, linear relation is good, blood sample loss is small, and the device is particularly suitable for being used for precious and trace samples.
Drawings
Fig. 1 is a front view of the present invention;
FIG. 2 is a front view of the cross section of the present invention;
FIG. 3 is a schematic view of the connection between the column body and the liquid collecting tube according to the present invention;
fig. 4 is a schematic view of part a of fig. 1.
In the figure: 1. a cylinder; 11. an external incision; 2. a closure cap; 3. a binding arm; 4. filtering paper; 5. a gasket; 6. righting the funnel-shaped device; 7. a liquid collection tube; 71. an inner incision; 8. a buckle mechanism; 81. a first vertical spring plate; 82. a transverse elastic sheet; 83. a second vertical spring plate; 84. an L-shaped elastic sheet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution:
as shown in fig. 1 and 4, a novel liquefaction column device for blood clot nucleic acid extraction, comprising:
Through adopting above-mentioned technical scheme, the closing cap 2 is opened through buckle mechanism 8 to the liquefaction post when using, adds the blood clot in the cylinder 1, then installs cylinder 1 and liquid collection pipe 7 through the screw thread, and through high-speed centrifugation, during liquid composition in the blood clot penetrated filter paper 4 and got into liquid collection pipe 7, impurity composition was detained in filter paper 4 top, accomplished the separation of blood and impurity.
As shown in fig. 2, the lower end opening of the column body 1 is contracted into a funnel shape.
By adopting the technical scheme, the surface relaxation degree of the filter paper 4 is ensured, the filter paper 4 is prevented from falling off, and the function of supporting the filter paper 4 is achieved.
As shown in fig. 4, preferably, the fastening mechanism 8 is composed of a first vertical elastic sheet 81, a horizontal elastic sheet 82, a second vertical elastic sheet 83 and an L-shaped elastic sheet 84, the first vertical elastic sheet 81 is vertically welded at the bottom of the right side of the sealing cover 2, one end of the horizontal elastic sheet 82 is vertically welded at the bottom of the first vertical elastic sheet 81, the second vertical elastic sheet 83 is vertically welded at the other end of the horizontal elastic sheet 82, the L-shaped elastic sheet 84 is welded at the middle position of the top of the column body 1, and at this time, the horizontal elastic sheet 82 and the second vertical elastic sheet 83 are attached to the L-shaped elastic sheet 84.
Through adopting above-mentioned technical scheme, through buckle mechanism 8, conveniently open and shut cylinder 1 adds the blood clot in cylinder 1.
As shown in fig. 3, an outer notch 11 is formed in the outer wall of the bottom of the column body 1, an external thread is integrally formed on the outer wall of the outer notch 11, an internal notch 71 is formed in the inner wall of the top of the liquid collecting pipe 7, an internal thread is integrally formed on the inner wall of the internal notch 71, the liquid collecting pipe 7 is installed at the bottom of the column body 1 through threaded connection, and at this time, the outer notch 11 and the internal notch 71 are overlapped.
By adopting the technical scheme, the liquid collecting pipe 7 is convenient to mount through threaded connection.
As shown in fig. 2, the gasket 5 is a plastic ring with an inner diameter smaller than the outer diameter of the filter paper 4, and the gasket 5 presses on the two layers of filter paper 4.
By adopting the technical scheme, the filter paper 4 is not easy to fall off through the gasket 5.
As shown in FIG. 2, the upright funnel 6 clamps the filter paper 4 and gasket 5 to the bottom of the column 1.
By adopting the technical scheme, the gasket 5 and the filter paper 4 are not easy to drop down through the upright funnel-shaped device 6.
The structure principle; as shown in figures 1-4, when the liquefaction column is used, the closing cover 2 is opened through the buckling mechanism 8, a blood clot is added into the column body 1, then the column body 1 and the liquid collecting pipe 7 are installed through threads, liquid components in the blood clot penetrate through the filter paper 4 and enter the liquid collecting pipe 7 through high-speed centrifugation, and impurity components are trapped above the filter paper 4 to finish the separation of blood and impurities.
In conclusion, when the liquefaction column is used, blood can be separated from impurities only by adding the blood clot into the column body 1 and then intercepting the impurities of the blood clot above the filter paper 4 through high-speed centrifugation.
The part not involved in the utility model is the same as the prior art or can be realized by adopting the prior art. Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A novel liquefaction column device for clot nucleic acid extraction, comprising:
cylinder (1), the top of cylinder (1) is equipped with closing cap (2), control is installed on the top right side of cylinder (1) buckle mechanism (8) that closing cap (2) opened and shut, the top left side of cylinder (1) with install between the left side of closing cap (2) and combine arm (3), filter paper (4) have been placed to the bottom inside of cylinder (1), filter paper (4) adopt two-layerly, filter paper (4) are the poroid structure of assembling of diameter 30-50 microns, the upper surface of filter paper (4) is equipped with packing ring (5), the inside integrated into one piece in bottom of cylinder (1) has just putting funnel-shaped device (6), liquid collecting pipe (7) are installed to the bottom of cylinder (1).
2. The novel liquefaction column device for blood clot nucleic acid extraction of claim 1, wherein: the lower end opening of the column body (1) is contracted into a funnel shape.
3. The novel liquefaction column device for blood clot nucleic acid extraction of claim 1, wherein: the buckle mechanism (8) is composed of a first vertical elastic sheet (81), a transverse elastic sheet (82), a second vertical elastic sheet (83) and an L-shaped elastic sheet (84), the first vertical elastic sheet (81) is vertically welded at the bottom of the right side of the sealing cover (2), one end of the transverse elastic sheet (82) is vertically welded at the bottom of the first vertical elastic sheet (81), the second vertical elastic sheet (83) is vertically welded at the other end of the transverse elastic sheet (82), the L-shaped elastic sheet (84) is welded at the middle position of the top of the cylinder (1), and the second vertical elastic sheet (83) is located on the inner side of the L-shaped elastic sheet (84).
4. The novel liquefaction column device for blood clot nucleic acid extraction of claim 1, wherein: outer incision (11) have been seted up to the bottom outer wall of cylinder (1), the outer wall integrated into one piece of outer incision (11) has the external screw thread, incision (71) in having seted up are seted up to the top inner wall of liquid collecting pipe (7), the inner wall integrated into one piece of interior incision (71) has the internal thread, liquid collecting pipe (7) are installed through threaded connection the bottom of cylinder (1), outer incision (11) with interior incision (71) coincidence.
5. The novel liquefaction column device for blood clot nucleic acid extraction of claim 1, wherein: the gasket (5) is arranged in a plastic circular ring shape, the inner diameter of the gasket is smaller than the outer diameter of the filter paper (4), and the gasket (5) is pressed on the two layers of the filter paper (4).
6. The novel liquefaction column device for blood clot nucleic acid extraction of claim 1, wherein: the filter paper (4) is clamped at the bottom of the column body (1) by the upright funnel-shaped device (6) and the gasket (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021882818.8U CN213232220U (en) | 2020-09-02 | 2020-09-02 | A novel liquefaction post device for blood clot nucleic acid draws |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021882818.8U CN213232220U (en) | 2020-09-02 | 2020-09-02 | A novel liquefaction post device for blood clot nucleic acid draws |
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| CN213232220U true CN213232220U (en) | 2021-05-18 |
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| CN202021882818.8U Active CN213232220U (en) | 2020-09-02 | 2020-09-02 | A novel liquefaction post device for blood clot nucleic acid draws |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
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2020
- 2020-09-02 CN CN202021882818.8U patent/CN213232220U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
| CN113717837B (en) * | 2021-08-13 | 2023-10-03 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification column |
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