CN213216842U - Confocal laser oral cavity micro-endoscope system - Google Patents

Abstract

The utility model belongs to the field of medical equipment, concretely relates to confocal laser oral cavity scope system in microscopy, confocal laser oral cavity scope in microscopy is integrated design, including electric connection's light source, leaded light/formation of image optic fibre bundle and scanning control unit in proper order, scanning control unit is connected with the probe, still includes electric connection's detection unit, image processing unit and image display in proper order, leaded light/formation of image optic fibre bundle with detection unit electric connection. Adopt the utility model discloses a confocal laser oral cavity microscopy endoscope system utilizes confocal laser microtechnology, can realize observing the organizational structure of each level of oral mucosa under the condition of not having the wound, both can reach the sensitivity and the specificity of histopathology rank, can avoid drawbacks such as the wound that traditional pathological examination caused again, has good clinical application prospect.

Description

Confocal laser oral cavity micro-endoscope system

Technical Field

The utility model belongs to the field of medical equipment, concretely relates to confocal laser oral cavity micro-endoscope system.

Background

Traditionally, the diagnosis of oral mucosal diseases has relied primarily on clinical examination and pathological biopsy. The clinical examination is a routine examination mode, including visual inspection, palpation, sniffing and the like, depends on the clinical experience of doctors, has a large degree of subjectivity, and some lesions with hidden and atypical manifestations are difficult to identify only by the clinical examination. Although the pathological examination is the gold standard for diagnosing oral mucosa diseases at present, as an invasive operation, the pathological examination process often causes different degrees of pain to patients, has certain risks, has limited acceptance of the patients, and has subjectivity in selecting biopsy parts. Therefore, the search for a novel method for diagnosing oral mucosal diseases, which is minimally invasive and has high sensitivity and specificity, is an urgent clinical need.

The confocal laser technology is a new imaging technology, the basic principle is that point illumination and point detection are realized by utilizing an illumination pinhole in front of a laser light source and a detection pinhole in front of a detector, and the light source, the detector and detected tissues are located at conjugate positions during imaging. The light from the light source is focused on a certain point of the focal plane through the illumination pinhole and the light guide system, and the reflected light forms a confocal point image at the detection pinhole through the light guide system. While light reflected by non-focal planes is blocked by the detection pinhole and cannot be imaged. During detection, the focal plane is positioned on different layers of the detected tissue, and corresponding optical cross-section images can be obtained layer by layer, so that the structure of each layer of the tissue can be observed under the condition of no wound, and the purpose of being similar to pathological examination is achieved.

The present confocal laser microscopy technology is widely applied to scientific research of cell biology, microbiology, developmental biology, genetics, neurobiology, physiology, pathology and other subjects, and has primary application in clinical diagnosis and treatment of multiple system diseases such as digestive tract, skin, urinary system, mammary gland and the like. In view of the particularity of the oral anatomy, none of the currently available confocal laser imaging devices can be used for examination of oral mucosa diseases. Therefore, the development of a confocal laser microscopy device specially used for oral mucosa examination is a problem to be solved in need of improvement of the diagnosis and treatment level of oral mucosa diseases and development of subjects.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a confocal laser oral cavity scope system organically combines confocal laser microscopy technique and oral cavity scope for the noninvasive inspection of oral cavity mucous membrane lesion.

The utility model adopts the following technical scheme: the utility model provides a confocal laser oral cavity micro-endoscope system, confocal laser oral cavity micro-endoscope is the integrated design, includes electric connection's light source, leaded light/formation of image optic fibre bundle and scanning control unit in proper order, scanning control unit is connected with the probe, still includes electric connection's detection unit, image processing unit and image display ware in proper order, leaded light/formation of image optic fibre bundle with detection unit electric connection.

Preferably, the light source comprises a laser, an illumination pinhole and a lens system which are arranged in sequence.

Preferably, the light guide/imaging fiber bundle includes an input fiber bundle, a main fiber bundle, an output fiber bundle and a beam splitter, the input fiber bundle is electrically connected to the light source and the beam splitter respectively, the main fiber bundle is electrically connected to the scanning control unit and the beam splitter respectively, and the output fiber bundle is electrically connected to the detection unit and the beam splitter respectively.

Preferably, the scanning control unit comprises an interface, an optical fiber channel and a focusing lens which are sequentially arranged, and further comprises a z-axis adjusting motor, an x/y-axis adjusting motor, a control panel and a microcomputer system, wherein the z-axis adjusting motor and the x/y-axis adjusting motor are both arranged outside the optical fiber channel, the z-axis adjusting motor is electrically connected with the control panel, the x/y-axis adjusting motor is electrically connected with the microcomputer system, and the control panel and the microcomputer system are both electrically connected with the image processing unit.

Preferably, the control panel comprises an equipment switch, a power supply display lamp, a depth adjusting wheel, a magnification button and an image shooting button, the depth adjusting wheel is connected with the z-axis adjusting motor, and the magnification button and the image shooting button are both electrically connected with the microcomputer system.

Preferably, the tail end of the probe is bent at a right angle, the inner wall of the probe is provided with a reflective layer, and the probe is connected with the scanning control unit and can rotate 360 degrees along the long axis.

Preferably, the detection unit comprises a photomultiplier tube, a detection pinhole and a focusing lens which are sequentially arranged, and the photomultiplier tube is electrically connected with the image processing unit.

Preferably, the image processing unit can assemble the digital signals of the confocal images into a confocal image of the whole focal plane, and the confocal image is displayed on the image display.

The utility model discloses in, the laser that the light source produced gets into leaded light/formation of image optic fibre bundle behind illumination pinhole and lens system, and rethread scanning control unit and probe focus on in some point in being surveyed the tissue, can adjust through the plane position of scanning control unit focusing plane degree of depth and focus point. The laser focused in the detected tissue excites the contrast agent which is coated on the surface of the tissue and absorbed by the tissue to generate reflected fluorescence, the reflected fluorescence reversely passes through the probe and the scanning control unit, enters the light guide/imaging optical fiber beam, is converged in the detection pinhole of the detection unit through the focusing lens to form a confocal point image, is received by the photomultiplier tube and converted into a digital signal, is transmitted to the image processing unit, is aggregated into a confocal image of the whole focal plane, and is finally displayed on the image display.

The focal plane can be positioned at different depths in the tested mucous membrane tissue through the scanning control unit and the image processing unit, so that the corresponding optical cross section image of the mucous membrane tissue can be obtained layer by layer and can be amplified to 800 times of 100 times, and the effect similar to pathological examination is realized.

Compared with the prior art, the beneficial effects of the utility model are that: adopt the utility model discloses a confocal laser oral cavity microscopy endoscope system utilizes confocal laser microtechnology, can realize observing the organizational structure of each level of oral mucosa under the condition of not having the wound, both can reach the sensitivity and the specificity of histopathology rank, can avoid drawbacks such as the wound that traditional pathological examination caused again, has good clinical application prospect.

Drawings

FIG. 1 is a schematic view of a confocal laser oral cavity endomicroscopy system of the present invention;

fig. 2 is a schematic diagram of a control panel included in the middle scanning control unit of the present invention.

The labels in the figures are: 1. a light source; 11. a laser; 12. illuminating the pinhole; 13. a lens system; 2. a light guiding/imaging fiber bundle; 21. inputting an optical fiber bundle; 22. a main optical fiber bundle; 23. outputting the optical fiber bundle; 24. a beam splitter; 3. a scanning control unit; 31. an interface; 32. a fiber channel; 33. a z-axis adjustment motor; 34. an x/y axis adjustment motor; 35. a focusing lens A; 36. a control panel; 361. a device switch; 362. a power supply display lamp; 363. a depth adjustment wheel; 364. a magnification button; 365. an image capture button; 37. a microcomputer system; 4. a probe; 5. a detection unit; 51. a photomultiplier tube; 52. detecting a pinhole; 53. a focusing lens B; 6. an image processing unit; 7. an image display.

Detailed Description

The following description is made with reference to the accompanying drawings and examples, but not to be construed as limiting the invention.

Fig. 1 is a schematic view of a confocal laser oral cavity endomicroscopy system of the present invention. The confocal laser oral cavity micro-endoscope system is designed in an integrated mode and comprises a light source 1, a light guide/imaging optical fiber bundle 2, a scanning control unit 3, a probe 4, a detection unit 5, an image processing unit 6 and an image display 7.

The light source 1 comprises a laser 11, an illumination pinhole 12 and a lens system 13. The laser 11 can emit laser with wavelength of 488nm, and enters the light guiding/imaging optical fiber bundle 2 after passing through the illumination pinhole 12 and the lens system 13.

The light guide/imaging fiber bundle 2 comprises an input fiber bundle 21, a main fiber bundle 22, an output fiber bundle 23 and a beam splitter 24; the input optical fiber bundle 21, the main optical fiber bundle 22 and the output optical fiber bundle 23 are all composed of about 30000 optical fibers, and the diameters of the optical fibers are about 1.9 μm; the beam splitter 24 can guide the laser light emitted by the light source 1 into the main optical fiber bundle 22 via the input optical fiber bundle 21, and can conduct the reflected fluorescence from the main optical fiber bundle 22 to the output optical fiber bundle 23 and into the detection unit 5.

The scanning control unit 3 includes an interface 31, an optical fiber channel 32, a z-axis adjusting motor 33, an x/y-axis adjusting motor 34, a focusing lens a35, a control panel 36 and a microcomputer system 37. The main optical fiber beam 22 can be connected to the scan control unit 3 through an interface 31 via an optical fiber channel 32. The z-axis adjusting motor 33 is controlled by an adjusting wheel in the control panel 36, and can adjust the optical fiber channel 32 and the connected main optical fiber beam 22 to move along the long axis z-axis direction, so as to adjust the depth of the focal plane, wherein the thickness of the optical layer of each scanning is 7 μm, and the scanning depth range in the mucosal tissue is 0-1000 μm. The x/y axis adjusting motor 34 is automatically controlled by a microcomputer system 37, and can adjust the optical fiber channel 32 and the main optical fiber beam 22 to move along the x axis and the y axis, so that the laser beam emitted by the optical fiber channel can be horizontally scanned on a focal plane, the scanning speed is 0.8-1.6 frames/second, the maximum scanning range diameter is 475 μm, and the lateral resolution is 0.7 μm. The control panel 36 and the microcomputer system 37 are connected to the image processing unit 6 through data lines.

The inner wall of the probe 4 is made of a reflective material, and the direction of laser light can be changed to enable the laser light to be perpendicular to the plane of the tail end of the probe to be emitted. The probe 4 is about 100mm long, has a bending degree of the tail end of about 90 degrees and a diameter of 10mm, and can rotate 360 degrees along the long axis, thereby being convenient for an operator to operate.

The detection unit 5 comprises a photomultiplier tube 51, a detection pinhole 52 and a focusing lens B53. The focusing lens B53 can form a confocal image of the reflected fluorescence from the light guiding/imaging fiber bundle at the detection pinhole 52. The photomultiplier 51 can convert the received confocal image into a digital signal, and transmit the digital signal to the image processing unit 6 through a data line.

The image processing unit 6 can assemble the digital signals of the confocal images into a confocal image of the whole focal plane through a computer system, and the confocal image is displayed on the image display 7 through a data line.

Fig. 2 is a schematic diagram of a control panel 36 included in the middle scanning control unit 3 of the present invention. The control panel 36 includes a device switch 361, a power display lamp 362, a depth adjustment wheel 363, a magnification button 364, and an image capture button 365. The depth adjustment wheel 363 can adjust the depth of the focal plane by controlling the z-axis motor 33. The magnification button 364 can be connected with the image processing unit 6 through the microcomputer system 37 to perform magnification adjustment of 100 times, 200 times, 400 times and 800 times on the confocal image. The image capturing button 365 can be connected to the image processing unit 6 through the microcomputer system 37 to capture and store the confocal image.

Confocal laser oral cavity endoscope system work flow do, laser that laser instrument 11 produced is behind illumination pinhole 12 and lens system 13, gets into leaded light/formation of image optic fibre and restraints 2, rethread scanning control unit 3 with probe 4 focuses on being surveyed some in the tissue, arouses the coating at the tissue surface and by the absorptive contrast medium of tissue, produces reflection fluorescence, and reverse through probe 4 and scanning control unit 3, get into leaded light/formation of image optic fibre and restraint 2, assembles in surveying pinhole 52 through focusing lens B53, forms the confocal image, is received by photomultiplier 51 and converts digital signal into, transmits to image processing unit 6, assembles into whole focal plane's confocal image show on image display 7. The depth of the focal plane and the plane position of the focal point can be adjusted by the scanning control unit 3, so that the focal plane is positioned at different depths in the mucous membrane tissue to be detected, thereby obtaining the corresponding optical cross-section image of the mucous membrane tissue layer by layer, and amplifying the confocal image to be 100-fold and 800-fold, thereby realizing the effect similar to pathological examination.

While the foregoing is directed to the preferred embodiment of the present invention, it will be understood that the invention is not limited thereto, and that various modifications and changes can be made by those skilled in the art without departing from the principles of the invention.

Claims (8)

1. A confocal laser oral cavity micro-endoscope system is characterized in that: confocal laser oral cavity micro-scope is integrated design, including electric connection's light source, leaded light/formation of image optic fibre bundle and scanning control unit in proper order, scanning control unit is connected with the probe, still includes electric connection's detection unit, image processing unit and image display in proper order, leaded light/formation of image optic fibre bundle with detection unit electric connection.

2. The confocal laser oral endomicroscopy system of claim 1, wherein: the light source comprises a laser, a lighting pinhole and a lens system which are sequentially distributed.

3. The confocal laser oral endomicroscopy system of claim 1, wherein: the light guide/imaging optical fiber bundle comprises an input optical fiber bundle, a main optical fiber bundle, an output optical fiber bundle and a beam splitter, wherein the input optical fiber bundle is electrically connected with the light source and the beam splitter respectively, the main optical fiber is electrically connected with the scanning control unit and the beam splitter respectively, and the output optical fiber bundle is electrically connected with the detection unit and the beam splitter respectively.

4. The confocal laser oral endomicroscopy system of claim 1, wherein: the scanning control unit comprises an interface, an optical fiber channel and a focusing lens A which are sequentially arranged, and further comprises a z-axis adjusting motor, an x/y-axis adjusting motor, a control panel and a microcomputer system, wherein the z-axis adjusting motor and the x/y-axis adjusting motor are arranged outside the optical fiber channel, the z-axis adjusting motor is electrically connected with the control panel, the x/y-axis adjusting motor is electrically connected with the microcomputer system, and the control panel and the microcomputer system are electrically connected with the image processing unit.

5. The confocal laser oral endomicroscopy system of claim 4, wherein: the control panel comprises an equipment switch, a power supply display lamp, a depth adjusting wheel, a magnification button and an image shooting button, the depth adjusting wheel is connected with the z-axis adjusting motor, and the magnification button and the image shooting button are both electrically connected with the microcomputer system.

6. The confocal laser oral endomicroscopy system of claim 1, wherein: the tail end of the probe is bent at a right angle, the inner wall of the probe is provided with a reflecting layer, and the probe is connected with the scanning control unit and can rotate 360 degrees along the long axis.

7. The confocal laser oral endomicroscopy system of claim 1, wherein: the detection unit comprises a photomultiplier tube, a detection pinhole and a focusing lens B which are sequentially arranged, and the photomultiplier tube is electrically connected with the image processing unit.

8. The confocal laser oral endomicroscopy system of claim 1, wherein: the image processing unit can assemble the digital signals of the confocal point images into a confocal image of the whole focal plane, and the confocal image is displayed on the image display.

Images