CN213121756U

CN213121756U - Chromatographic mass spectrometry detection amino metabolite sample configuration kit

Info

Publication number: CN213121756U
Application number: CN202021759217.8U
Authority: CN
Inventors: 张磊; 李振
Original assignee: Nanjing Lvzheng Biotechnology Co Ltd
Current assignee: Nanjing Lvzheng Biotechnology Co Ltd
Priority date: 2020-08-21
Filing date: 2020-08-21
Publication date: 2021-05-04
Anticipated expiration: 2030-08-21

Abstract

The utility model discloses a chromatographic mass spectrometric detection amino metabolite sample configuration kit, which comprises a kit main body, an integrated high-efficiency liquid-transferring device and an aperture-adjustable reagent pipe orifice, one side of the inner top of the kit main body is fixedly connected with the integrated high-efficiency liquid-transferring device, the other side of the inner top of the kit main body is movably connected with the aperture-adjustable reagent pipe orifice, the back of the kit main body is movably connected with a reagent box cover, the back of the inner side of the kit main body is fixedly connected with a pipette bundling lapping rod, the bottom of the kit main body is connected with a nested buffer sleeve in a nested way, the nested buffer sleeve well embodies the buffering protection of the kit, the pipette bundling lapping rod well embodies the effective bundling of the kit, the integrated high-efficiency liquid-transferring device well embodies the integrated convenience of the device, and the aperture-adjustable reagent pipe orifice, the method is suitable for the use of analytical chemistry technology and has wide development prospect in the future.

Description

Chromatographic mass spectrometry detection amino metabolite sample configuration kit

Technical Field

The utility model relates to an analytical chemistry technical field specifically is a chromatogram mass spectrum detects amino metabolite sample configuration kit.

Background

The spectrum mass spectrum is widely used for targeted accurate quantitative detection of biological samples, amino metabolites are low molecular weight organic compounds with biological activity and nitrogen, and comprise common 20 protein amino acids, amino acids with sulfydryl and disulfide bonds, polypeptides, catecholamine, other amino acids and group derivatives, other biogenic amines and the like, and often have very important physiological functions, the quantitative accurate detection of the amino metabolites in the biological samples is very important for biological research and discovery of disease markers, and the detection requirements of scientific research institutes are very urgent.

The test tube in current kit all is fixed aperture, can't adjust the aperture according to the needs of reality, and the suitability is not enough, and current kit need carry the pipettor at any time with the help of the pipettor in addition when carrying out reagent transfer configuration, comparatively troublesome, and an organic whole nature is not enough, because the reagent sample is comparatively noble and fragile, takes place to collide with when transporting and causes the loss easily, and the protecting against shock nature of current kit is not enough.

Therefore, how to design a reagent kit for preparing amino metabolite samples by chromatographic mass spectrometry becomes a problem to be solved currently.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a chromatogram mass spectrum detects amino metabolite sample configuration kit to what provide does not possess aperture adjustment nature and can't independently move the liquid operation in solving above-mentioned background art, the not enough problem of wholeness.

In order to achieve the above object, the utility model provides a following technical scheme: a reagent kit for preparing amino metabolite samples for chromatographic mass spectrometry comprises a reagent kit main body, an integrated efficient liquid transfer device and an adjustable-aperture reagent tube orifice, wherein one side of the inner top of the reagent kit main body is fixedly connected with the integrated efficient liquid transfer device, the other side of the inner top of the reagent kit main body is movably connected with the adjustable-aperture reagent tube orifice, the back of the reagent kit main body is movably connected with a reagent box cover, one side of the inner bottom of the reagent kit main body is fixedly connected with a negative pressure suction apparatus, the inner bottom of the negative pressure suction apparatus is fixedly connected with a negative pressure pump, one side of the inner top of the negative pressure suction apparatus is fixedly connected with a negative pressure inlet, the other side of the inner top of the negative pressure suction apparatus is fixedly connected with a liquid outlet, the inner side of the negative pressure inlet is, the test tube presss from both sides the both sides mouthful both sides fixedly connected with and presss from both sides a mouthful movable wall, the orificial inside inboard swing joint of aperture adjustable reagent has the expansion and contraction compressed spring, the one end fixedly connected with of test tube clamp mouth adjusts the pulling rod, adjust the inside inboard fixedly connected with pulling rod spacing hole of pulling rod, the inside inboard through connection who pulls the spacing hole of rod has the positioning spring trip.

Preferably, a pipette bundling hasp rod is fixedly connected to the rear part of the inner side of the kit body.

Preferably, the middle part of the front surface of the kit main body is fixedly connected with a kit embedded glass.

Preferably, the bottom of the kit body is connected with a nesting buffer sleeve in a nesting mode.

Preferably, the integrated high-efficiency liquid transfer device is formed by combining a negative pressure suction apparatus on one side of the bottom inside the kit body, a negative pressure pump on the bottom inside the negative pressure suction apparatus, a negative pressure liquid inlet on one side of the top inside the negative pressure suction apparatus, a liquid outlet on the other side of the top inside the negative pressure suction apparatus and a liquid transfer rubber tube on the inner side inside the negative pressure liquid inlet.

Preferably, the reagent mouth of pipe that the aperture is adjustable is pressed from both sides the mouth by the test tube of inside middle part, and the mouth activity wall is pressed from both sides to the test tube presss from both sides the mouth both sides, and inside inboard expansion compresses tightly the spring, and the regulation pulling rod of test tube clamp mouth one end adjusts the pulling rod and moves the inside inboard spacing hole of pulling rod spacing hole and the inside inboard location spring fastening of pulling rod spacing hole and make up jointly.

Compared with the prior art, the beneficial effects of the utility model are that:

1. according to the preparation kit for detecting the amino metabolite sample by the chromatographic mass spectrometry, the integrated high-efficiency liquid transfer device is arranged, a reagent is sucked along the negative pressure liquid inlet by the device through the pump suction force of the negative pressure pump, and then the reagent is sucked into the other reagent tube through the liquid transfer rubber tube at the liquid outlet, so that the operation is simple and rapid, a liquid transfer device is not required to be additionally arranged, and the integrated convenience of the device is well reflected;

2. this kind of chromatogram mass spectrum detects amino metabolite sample configuration kit, inside is provided with mobilizable test tube and presss from both sides the mouth, during the regulation, adjust the pulling rod through manual drawing and drive the test tube and press from both sides the mouth activity of contracting at the hole inslot along pressing from both sides mouthful movable wall, the test tube presss from both sides the mouth and sticiss slowly expanding and contracting under sticising of spring expanding and contracting, after expanding and contracting to the aperture that accords with, go into the positioning spring bayonet catch card to adjust the pulling rod in the pulling rod spacing hole carry on spacing fixed can, high convenience and fastness, fine embodiment this design adjustability and practicality.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic sectional structure view of the main body of the kit of the present invention;

FIG. 3 is a schematic sectional view of the aperture-adjustable reagent tube orifice of the present invention;

fig. 4 is an enlarged schematic view of the position a of the present invention.

In the figure: 1. the reagent box comprises a reagent box body, 2, an integrated high-efficiency pipetting device, 3, a reagent pipe orifice with an adjustable aperture, 301, a test tube clamping orifice, 302, a clamping orifice movable wall, 303, an expansion compression spring, 304, an adjusting pulling rod, 305, a pulling rod limiting hole, 306, a positioning spring clamping bolt, 4, a reagent box cover, 5, a pipette bundling lapping rod, 6, embedded glass of the reagent box, 7, a nested buffer sleeve, 8, a negative pressure aspirator, 9, a negative pressure pump, 10, a negative pressure liquid inlet, 11, a liquid outlet, 12 and a pipetting rubber tube.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a chromatographic mass spectrometry detection amino metabolite sample configuration kit comprises a kit body 1, an integrated efficient liquid transfer device 2 and an aperture-adjustable reagent tube orifice 3, wherein one side of the inner top of the kit body 1 is fixedly connected with the integrated efficient liquid transfer device 2, the other side of the inner top of the kit body 1 is movably connected with the aperture-adjustable reagent tube orifice 3, the back of the kit body 1 is movably connected with a reagent box cover 4, one side of the inner bottom of the kit body 1 is fixedly connected with a negative pressure aspirator 8, the inner bottom of the negative pressure aspirator 8 is fixedly connected with a negative pressure pump 9, one side of the inner top of the negative pressure aspirator 8 is fixedly connected with a negative pressure liquid inlet 10, the other side of the inner top of the negative pressure aspirator 8 is fixedly connected with a liquid outlet 11, the inner side of the negative pressure liquid inlet 10 is connected with a liquid transfer rubber tube 12 in, the both sides fixedly connected with of test tube clamp mouth 301 press from both sides a mouthful movable wall 302, and the inside inboard swing joint of the adjustable reagent mouth of pipe 3 in aperture has the expansion and contraction to sticiss spring 303, and the one end fixedly connected with of test tube clamp mouth 301 adjusts pulling rod 304, adjusts the inside inboard fixedly connected with pulling rod limiting hole 305 of pulling rod 304, and the inside inboard through connection that pulls rod limiting hole 305 has positioning spring bolt 306.

Preferably, the inboard rear portion fixedly connected with pipette of kit main part 1 is taken up and is taken pole 5, because this kit is provided with the pipette, the rubber tube is placed at will and is polluted and tie in the box easy production, through setting up pipette and taking up and take pole 5, pipette is taken up and is taken pole 5 for fine taking up design, medical staff can deposit not the time spent, take the orderly winding of pipette and put can be fine prevent to tie in and the pipette pollutes on pipette is taken up and take pole 5, fine embodiment the effective bundling nature of this kit.

Preferably, the embedded glass 6 of kit of preceding middle part fixedly connected with of kit main part 1, because the kit is for sealing the design, medical staff can't directly observe the storage situation of inside reagent, need take out reagent, comparatively troublesome, through setting up the embedded glass 6 of kit, the embedded glass 6 of kit is fine embedded glass material, and medical staff's the embedded glass 6 of kit of accessible is observed the reagent storage situation of the box inside in real time to fine embodiment the observation convenience of kit.

Preferably, the bottom of the kit main body 1 is connected with the nested buffer sleeve 7 in a nested manner, and because reagent samples are expensive and fragile, the reagent samples are collided during transportation to easily cause loss, the impact protection performance of the existing kit is not sufficient, and the nested buffer sleeve 7 is a good foam buffer layer through the nested buffer sleeve 7, so that the damage to the kit caused by external impact can be well buffered, and the buffer protection performance of the kit is well embodied.

Preferably, the integrated high-efficiency liquid transfer device 2 is formed by combining a negative pressure suction apparatus 8 at one side of the inner bottom of the kit body 1, a negative pressure pump 9 at the inner bottom of the negative pressure suction apparatus 8, a negative pressure liquid inlet 10 at one side of the inner top of the negative pressure suction apparatus 8, a liquid outlet 11 at the other side of the inner top of the negative pressure suction apparatus 8 and a liquid transfer rubber tube 12 at the inner side of the negative pressure liquid inlet 10, when the reagent is transferred and configured in the conventional kit, an additional pipettor needs to be carried at any time, which is troublesome and has insufficient integrity, by arranging the integrated high-efficiency liquid transfer device 2, the medical staff can suck the reagent from the reagent tube along the negative pressure liquid inlet 10 through the liquid transfer rubber tube 12 in the kit through the pump suction force of the negative pressure suction apparatus 8 in the kit, and then suck, the operation is simple and rapid, a liquid transfer device does not need to be additionally configured, and the integrated convenience of the device is well embodied.

Preferably, the aperture-adjustable reagent tube orifice 3 is formed by combining a test tube clamping orifice 301 in the middle of the inside, clamping orifice movable walls 302 on two sides of the test tube clamping orifice 301, an expansion and contraction compression spring 303 on the inner side of the inside, an adjusting pulling rod 304 at one end of the test tube clamping orifice 301, an adjusting pulling rod limiting hole 305 on the inner side of the adjusting pulling rod 304 and a positioning spring clamping bolt 306 on the inner side of the pulling rod limiting hole 305, test tubes in the existing reagent box are all fixed in aperture and cannot be adjusted in aperture according to actual requirements, the applicability is insufficient, by arranging the aperture-adjustable reagent tube orifice 3, the movable test tube clamping orifice 301 is arranged in the aperture-adjustable reagent tube orifice 3, during adjustment, the test tube clamping orifice 301 is driven to expand and contract in a hole groove along the clamping orifice movable walls 302 by manually stretching and adjusting the pulling rod 304, the test tube clamping orifice 301 is slowly expanded and contracted under the compression of the expansion and contraction spring 303, and contraction are carried out after being expanded, the positioning spring clamping bolt 306 is clamped into the pulling rod limiting hole 305 in the adjusting pulling rod 304 for limiting and fixing, so that the device is convenient and quick, and the adjustability and the practicability of the design are well embodied.

The working principle is as follows: firstly, by arranging the pipette bundling taking rod 5, the pipette bundling taking rod 5 is a good bundling rod design, so that medical staff can orderly wind and take pipettes on the pipette bundling taking rod 5 when the pipettes are not used for storage, knotting and pipette pollution can be well prevented, and the effective bundling performance of the kit is well reflected;

then, by arranging the embedded glass 6 of the kit, the embedded glass 6 of the kit is a good embedded glass material, and medical staff can observe the storage condition of the reagent in the kit in real time through the embedded glass 6 of the kit, so that the observation convenience of the kit is well embodied;

then, by arranging the nested buffer sleeve 7, the nested buffer sleeve 7 is a good foam buffer layer, so that the damage of external collision to the kit can be well buffered, and the buffering protection performance of the kit is well embodied;

next, by arranging the integrated high-efficiency liquid transfer device 2, the device is provided with a negative pressure aspirator 8 in the kit, and through the pumping force of a negative pressure pump 9, medical staff can suck a reagent from a reagent tube along a negative pressure liquid inlet 10 through a liquid transfer rubber tube 12 and then suck the reagent into another reagent tube through the liquid transfer rubber tube 12 at a liquid outlet 11, so that the operation is simple and rapid, a liquid transfer device is not required to be additionally arranged, and the integrated convenience of the device is well embodied;

finally, through setting up adjustable reagent mouth of pipe 3 in aperture, the inside mobilizable test tube that is provided with of adjustable reagent mouth of pipe 3 in aperture presss from both sides mouthful 301, during the adjustment, adjust pulling rod 304 through manual drawing and drive test tube and press from both sides mouthful activity wall 302 and expand the activity of contracting in the hole groove, test tube presss from both sides mouth 301 and compresses tightly the contracting slowly under sticising of spring 303 expanding and contracting, after expanding and contracting to the aperture that accords with, go into positioning spring bayonet catch 306 card and adjust pulling rod spacing hole 305 in the pulling rod 304 in carry out spacing fixed can, high convenience and fast, fine embodiment the controllability and the practicality of this design, this is exactly this kind of chromatography mass spectrometry amino metabolite sample configuration kit's theory of operation.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a chromatogram mass spectrum detects amino metabolite sample configuration kit, includes kit main part (1), high-efficient liquid-transfering device of integration (2) and the adjustable reagent mouth of pipe of bore (3), its characterized in that: the integrated efficient liquid transfer device (2) is fixedly connected to one side of the inner top of the kit main body (1), the other side of the inner top of the kit main body (1) is movably connected with a reagent pipe orifice (3) with an adjustable aperture, one side of the inner bottom of the kit main body (1) is fixedly connected with a negative pressure aspirator (8), the inner bottom of the negative pressure aspirator (8) is fixedly connected with a negative pressure pump (9), one side of the inner top of the negative pressure aspirator (8) is fixedly connected with a negative pressure liquid inlet (10), the other side of the inner top of the negative pressure aspirator (8) is fixedly connected with a liquid outlet (11), the inner side of the negative pressure liquid inlet (10) is in through connection with a liquid transfer rubber pipe (12), the middle part of the inner part of the reagent pipe orifice (3) with the adjustable aperture is movably connected with a test tube clamping opening (301), and movable clamping opening, the inside inboard swing joint of the adjustable reagent mouth of pipe in aperture (3) has the expansion and contraction to sticis spring (303), the one end fixedly connected with of test tube clamp mouth (301) is adjusted and is moved pole (304), the inside inboard fixedly connected with of adjusting the pulling pole (304) is moved the pole spacing hole (305), the inside inboard through connection that the pulling moved the pole spacing hole (305) has positioning spring bolt (306).

2. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the rear of the kit body (1) is movably connected with a kit cover (4).

3. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the rear part of the inner side of the kit main body (1) is fixedly connected with a pipette bundling hasp rod (5).

4. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the middle part of the front surface of the kit main body (1) is fixedly connected with a kit embedded glass (6).

5. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the bottom of the kit main body (1) is connected with a nesting buffer sleeve (7) in a nesting way.

6. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the integrated high-efficiency liquid transfer device (2) is formed by jointly combining a negative pressure suction apparatus (8) on one side of the bottom inside a kit body (1), a negative pressure pump (9) on the bottom inside the negative pressure suction apparatus (8), a negative pressure liquid inlet (10) on one side of the top inside the negative pressure suction apparatus (8), a liquid outlet (11) on the other side of the top inside the negative pressure suction apparatus (8) and a liquid transfer rubber tube (12) on the inner side of the top inside the negative pressure liquid inlet (10).

7. The reagent kit for preparing amino metabolite sample for chromatography-mass spectrometry as claimed in claim 1, wherein: the adjustable reagent mouth of pipe (3) in aperture is by test tube clamp mouth (301) of inside middle part, and the test tube presss from both sides the mouth movable wall (302) of pressing from both sides of mouth (301) both sides, and the inside inboard expansion compresses tightly spring (303), and the regulation of test tube clamp mouth (301) one end pulls pole (304), adjusts that the pulling pole (304) inside inboard pulling pole spacing hole (305) and the pulling pole spacing hole (305) inside inboard positioning spring gim peg (306) make up jointly and form.