CN212955129U - Mouth suction tube for sucking oocyte or embryo - Google Patents
Mouth suction tube for sucking oocyte or embryo Download PDFInfo
- Publication number
- CN212955129U CN212955129U CN202021054096.7U CN202021054096U CN212955129U CN 212955129 U CN212955129 U CN 212955129U CN 202021054096 U CN202021054096 U CN 202021054096U CN 212955129 U CN212955129 U CN 212955129U
- Authority
- CN
- China
- Prior art keywords
- tube
- latex
- glass tube
- latex tube
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 29
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 24
- 239000004816 latex Substances 0.000 claims abstract description 59
- 229920000126 latex Polymers 0.000 claims abstract description 59
- 239000011521 glass Substances 0.000 claims abstract description 48
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 7
- 238000005452 bending Methods 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 7
- 229920001971 elastomer Polymers 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 8
- 238000012546 transfer Methods 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 210000001771 cumulus cell Anatomy 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a suction tube for sucking oocytes or embryos. The oral siphon comprises five parts, namely a glass tube, a coarse latex tube, a filter, a fine latex tube and a pipette head, wherein the glass tube is bent to form a specific angle through processing, so that lossless and rapid suction and transfer are realized, the success rate of a downstream experiment is increased, meanwhile, each part is convenient to disassemble, simple to assemble and complete in sterilization, and smooth operation of subsequent oocyte or embryo can be ensured. The utility model discloses the equipment is simple, and the equipment part all belongs to aseptically, is applicable to different personnel and operates to the operation is fast, short in time, has guaranteed to oocyte or embryo quick, aseptic operation, very big reduction the oocyte of external treatment or the death rate of embryo.
Description
Technical Field
The invention relates to the field of life science, in particular to a mouth suction tube for sucking oocytes or embryos.
Background
The removal of embryonic cells or oocytes in cell experiments is cumbersome and difficult to perform completely aseptically. In the experiment, the oocyte or the embryo cell is small, so that the embryo or the embryo cell can only be operated under a body type microscope, a large amount of embryos are not easy to be rapidly moved, and the embryo or the oocyte is not easy to be finely operated by a common embryo operating tube (such as rinsing of the oocyte and peeling of cumulus cells), so that the normal operation of the follow-up experiment is not facilitated.
In addition, the adoption of expensive ovum or embryo transferrer and the matching of special ovum or embryo transfer tube, especially disposable consumables, all can increase the economic cost. The adoption of an integrated device with one end connected with a pasteur pipette and the other end connected with a silicone tube with a filter has the problems of high technical requirements on operators and incapability of quickly and massively carrying out oocyte or embryo operation. Meanwhile, other oral pipettes are easily contaminated by saliva of an operator during use, or a sterile filtering device needs to be additionally added, so that the manufacturing cost is obviously increased. In addition, the traditional suction pipe has the defects that the hose is directly communicated with the embryo glass tube, the gas blown out from the mouth of the traditional suction pipe is directly contacted with the embryo culture solution, the aseptic operation cannot be ensured, and the suction pipe has poor operation accuracy due to the difficulty in stable control of the airflow.
Disclosure of Invention
To above problem, the utility model provides a novel aseptic suction tube of absorbing oocyte or embryo, the suction tube of mouth of design can be used for embryo or oocyte to move fast, a large amount of asepsis and get, can effectively blow to beat and the rinsing oocyte simultaneously, can effectively ensure the success of low reaches experiment and go on.
The utility model discloses the equipment is simple, and convenient operation can carry out quick absorption and transfer to a large amount of oocytes or embryo, and the kou hand is used simultaneously and has improved the efficiency when moving oocyte or embryo, and to oocyte or embryo not damaged, and the operation time spent is few, very big shortening the time that oocyte or embryo stop outside, increased the success rate of low reaches experiment. In addition, the manufactured oral suction tube has the advantages of simple manufacture, cheap materials, easy acquisition, repeated utilization and the like.
The utility model discloses a mouth suction pipe, which comprises a glass pipe, a coarse latex pipe, a filter, a fine latex pipe and a pipette head; the glass tube is divided into a straight section and a bent section; the straight section and the bending section of the glass tube are integrated; the straight section of the glass tube and the bent section of the glass tube form an included angle of 30 degrees, the bent section of the glass tube is variable in diameter, the upper part of the bent section is an expansion part, the lower part of the bent section is gradually reduced along with the inner diameter to form a sharp-mouth end, and the inner diameter of the sharp-mouth end is 25-35 mu m; the expansion part provides possibility for storing a large number of cells and provides basic conditions for subsequent large-scale operation.
The straight section of the glass tube is connected with one end of a thick latex tube, the other end of the thick latex tube is connected with a thick port of a filter, the other end of the filter, namely a thin port of the filter, is connected with one end of a thin latex tube, and the other end of the thin latex tube is connected with a tip end of a pipette head; the straight section of the glass tube, the thick latex tube, the thin latex tube and the filter connecting opening are coaxial. The thin latex tube is a bicycle valve core latex tube, the outer diameter of the thin latex tube is 3mm, and the inner diameter of the thin latex tube is 1.5 mm; the pipette tip is a hollow conical cylinder made of polypropylene materials, and sterile absorbent cotton is placed in the conical cylinder.
The glass tube, the coarse latex tube, the filter, the fine latex tube and the pipette head are detachable and are sterilized before use by split charging or integral packaging, each component of the oral suction tube is sterile, and each component is assembled and connected in an elastic sealing mode through the rubber tube. The coarse latex tube in the oral suction tube is a buffer section and is made of silica gel or a rubber hose.
The filter size is preferably 0.22 μm, and the pipette tip is preferably 1 mL.
The preparation method of the oral suction tube mainly comprises the following steps: in the sterilization treatment, the glass tube is wrapped with tinfoil paper after being cleaned, two latex tubes (thick and thin) and two gun heads are packaged by an autoclave bag, sterilized in an autoclave at 120 ℃ for 30 minutes, and then taken out to be dried in a drying oven at 50 ℃ overnight. The above sterilized material and 0.22 μm aseptically packed filter were brought into the cell laminar flow chamber for subsequent operations. Manufacturing a bending section: in the cell chamber, an operator holds the sterilized ophthalmological forceps with the left hand, holds the sterilized glass tube with the right hand and preheats the thin end of the glass tube on an alcohol lamp, puts the thin end of the glass tube on the alcohol lamp for burning, clamps the tail end with the ophthalmological forceps with the left hand immediately after the thin end turns red and soft, and rapidly stretches the glass tube with proper force with the direction forming an included angle of 30 degrees with the glass tube to ensure that the length of the thin end is 6-8cm, and finally performs fracture by using a thin grinding wheel, wherein the length of the thin end after fracture is kept at 4-5cm, and the inner diameter is about 30 mu m. The utility model discloses glass manages crooked section is the drawing formation naturally, and glass manages the nature bending, is an organic whole and link up, does not have drop, gas tightness scheduling problem.
The length ratio of the straight section of the glass tube to the bent section of the glass tube is 1: 2.5-5, preferably 1: 3.5; the length ratio of the coarse latex tube to the fine latex tube is 1:1.5-3, preferably 1: 2; the ratio of the outer diameter of the coarse latex tube to the outer diameter of the fine latex tube is (2-3): 1; the ratio of the inner diameter of the thick latex tube to the inner diameter of the thin latex tube is 3-5: 1.
the starting point of the bending section can be used as the rotation center of the bending section, and the angle of the bending section can be 0-90 degrees, including 30 degrees, 45 degrees and 90 degrees according to the habit of an operator. The starting point of the bending section is collinear with the center of the straight section of the glass tube.
The utility model has the advantages as follows:
(1) in oocyte or embryo transfer experiments, large numbers of embryos or oocytes can be transferred quickly. In addition, the mouth and the hand are used simultaneously, the operation is convenient and simple, and cumulus cells around the oocytes can be easily removed by using the oral pipette.
(2) The connection of the five parts of the oral suction tube adopts seamless elastic sealing, and the oral suction tube is detachable, simple to assemble, low in preparation cost and capable of being rapidly popularized in a large scale; and the aseptic operation is strengthened, and the sterilization standard is efficiently realized.
(3) The oral suction tube is designed with double-layer filtration, aseptic absorbent cotton and a 0.22 mu m filter effectively prevent pollution, and improve the experimental accuracy.
(4) By adjusting the length of the liquid gun head and the thin latex tube, the negative pressure of the suction tube can be adjusted, so that the suction tube is suitable for the habit of each operator and is more humanized.
(5) The glass tube of the oral suction tube is sterilized by alcohol and can be repeatedly used; the mouth suction pipe gun head can be frequently replaced, so that the gun head is suitable for being operated by multiple persons and can avoid pollution.
(6) The bent section of the glass tube of the oral suction tube, the coarse latex tube, the fine latex tube, the filter and the sterile absorbent cotton play roles in buffering and stabilizing air flow; meanwhile, the pollution is prevented, and meanwhile, the impact of strong airflow can be relieved, so that the damage to embryos and oocytes caused by overlarge airflow pressure during suction is avoided.
(7) The utility model can be adjusted and changed according to the needs, for example, the filtering connecting port can be designed with a circle of annular bulge according to the actual needs, so as to strengthen the anti-skid and sealing functions; the manually drawn glass straws of various specifications and shapes can be connected according to different tests and individual requirements. Therefore, the practicability is stronger, and the experiment cost is saved.
Drawings
Fig. 1 is an overall schematic view of the oral siphon of the present invention. Wherein, 1-pipette head, processed 2-thin latex tube, 3-filter, 4-thick latex tube, 5 glass tube; 6-sterile absorbent cotton, 7-pipette tip end, 8-filter narrow opening, 9-filter wide opening, 10-thick latex tube and glass tube chain interface, 11-glass tube bending section tip end and 12-bending section starting point.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples.
The length of the bent end of the glass tube manually drawn by the alcohol lamp is about 5cm, the inner diameter of the sharp mouth port is about 30 mu m, and the included angle of 30 degrees is formed between the straight section end of the glass tube and the bent section of the glass tube after treatment. The parameters of the coarse latex tube in the latex tube are as follows: the outer diameter is 8mm, the inner diameter is 6mm, and the length is about 10 cm; the thin latex tube is a bicycle valve core rubber tube, the outer diameter is 3mm, the inner diameter is 1.5mm, and the length is 20 cm. A sterile filter of 0.22 mu m and a 1mL blue pipette tip are selected, and a cluster of sterile absorbent cotton is plugged into the pipette tip.
As shown in fig. 1, the oral siphon of the present embodiment includes a glass tube, a coarse latex tube, a filter, a fine latex tube, and a pipette tip, which are sequentially connected, and the five are sleeved and fitted, and can be freely detached, and the joints of the five remain sealed. The straight section of the glass tube is connected with one end of a coarse latex tube (the coarse latex tube is connected with a glass tube chain interface), the other end of the coarse latex tube is connected with a coarse opening of a filter, the other end of the filter, namely a fine opening of the filter, is connected with one end of a fine latex tube, and the other end of the fine latex tube is connected with a tip end of a pipette head; the straight section of the glass tube, the thick latex tube, the thin latex tube and the filter connecting opening are coaxial;
during operation, a collecting vessel containing oocytes or embryos is placed under a stereomicroscope, an operator enables a gun head of a connecting latex tube filled with absorbent cotton to be wide-mouthed and contained in the mouth, the collecting vessel is moved under the microscope by the left hand, the thin end of the glass tube is moved into collecting liquid by the right hand holding the glass tube, before the oocytes are sucked, a small amount of liquid is sucked into the tip part of the thin tube by the tip of the thin tube, then the oocytes are sucked one by one under the stereomicroscope and placed into a new culture vessel containing culture liquid. After the operation was completed, the glass tube was rinsed with 75% alcohol and placed in a laminar flow chamber tube rack for the next use. The latex tube of connecting the filter after dismantling is arranged in clean receiver.
The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; all the equivalent structures or equivalent flow changes made by the content of the specification of the utility model, or the direct or indirect application in other related technical fields, are included in the scope of the utility model.
Claims (3)
1. A mouth pipette for aspirating oocytes or embryos, comprising: the oral suction tube comprises a glass tube, a coarse latex tube, a filter, a fine latex tube and a pipette head; the glass tube is divided into a straight section and a bent section; the straight section and the bending section of the glass tube are integrated; the straight section of the glass tube and the bent section of the glass tube form an included angle of 30 degrees, the bent section of the glass tube is variable in diameter, the upper part of the bent section is an expansion part, the lower part of the bent section is gradually reduced along with the inner diameter to form a sharp-mouth end, and the inner diameter of the sharp-mouth end is 25-35 mu m; the straight section of the glass tube is connected with one end of a thick latex tube, the other end of the thick latex tube is connected with a thick port of a filter, the other end of the filter, namely a thin port of the filter, is connected with one end of a thin latex tube, and the other end of the thin latex tube is connected with a tip end of a pipette head; the straight section of the glass tube, the thick latex tube, the thin latex tube and the filter connecting opening are coaxial; the thin latex tube is a bicycle valve core latex tube, the outer diameter of the thin latex tube is 3mm, and the inner diameter of the thin latex tube is 1.5 mm; the pipette tip is a hollow conical cylinder made of polypropylene materials, and sterile absorbent cotton is filled in the conical cylinder.
2. The pipette for oocyte or embryo aspiration according to claim 1, wherein: the glass tube, the thick latex tube, the filter, the thin latex tube and the pipette head are detachable and are sterilized before use through split charging or integral charging, each component of the pipette is sterile, and each component is assembled and connected in an elastic sealing mode through the rubber tube.
3. The oral pipette for oocyte or embryo suction according to claim 1, wherein the crude latex tube in the oral pipette is a buffer section and is made of silica gel or a rubber hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021054096.7U CN212955129U (en) | 2020-06-10 | 2020-06-10 | Mouth suction tube for sucking oocyte or embryo |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021054096.7U CN212955129U (en) | 2020-06-10 | 2020-06-10 | Mouth suction tube for sucking oocyte or embryo |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212955129U true CN212955129U (en) | 2021-04-13 |
Family
ID=75385144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021054096.7U Expired - Fee Related CN212955129U (en) | 2020-06-10 | 2020-06-10 | Mouth suction tube for sucking oocyte or embryo |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212955129U (en) |
-
2020
- 2020-06-10 CN CN202021054096.7U patent/CN212955129U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207418761U (en) | Breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus | |
| CN212955129U (en) | Mouth suction tube for sucking oocyte or embryo | |
| CN207871198U (en) | A kind of paediatrics sputum aspirator | |
| CN109091170A (en) | A kind of Neurology specimen sampler | |
| CN210560431U (en) | Simple embryo suction device | |
| CN208524993U (en) | A kind of micromanipulation embryo transfer device | |
| CN210237620U (en) | Egg picking needle for IVF-ET | |
| CN113029912B (en) | Bacterial virus filtration efficiency tester and operation method thereof | |
| CN206843460U (en) | Device is searched outside a kind of embryoid body | |
| CN218539671U (en) | Embryo transfer device of adjustable negative pressure | |
| CN210419911U (en) | Device for sucking oocyte or embryo | |
| CN203436630U (en) | Injection device and counter pressure type cefoxitin bottle assembly | |
| CN209778837U (en) | ovum needle is picked up to pig's ovum | |
| CN212581893U (en) | Hand-held embryo and oocyte operating means | |
| CN110257229A (en) | It is a kind of simply to inhale fetus device and preparation method thereof | |
| CN204569360U (en) | Medical ampoule glass bottle clip bottle opener | |
| CN202740040U (en) | Novel in-vitro embryo/egg collecting device | |
| CN203440377U (en) | Device for aseptically collecting animal cells | |
| CN202047058U (en) | Dispersing and split charging device for passage cells | |
| CN215480862U (en) | Special pasteur straw for tissue blocks | |
| CN215627951U (en) | Gamete and embryo operating means | |
| CN220982796U (en) | Aseptic sampling device of chemical medicine pharmacy | |
| CN108992075A (en) | The blood collecting tubulasis of disposable polyethylene alcohol material | |
| CN213416878U (en) | Microbial coater capable of absorbing and releasing bacteria liquid | |
| CN220450184U (en) | Cell supernatant negative pressure collecting bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210413 |