CN212845220U - Column selection valve and chromatographic column combined device - Google Patents

Abstract

The utility model discloses a column selection valve and chromatographic column combined device, the stator has a centre bore, a plurality of holes are distributed with equal angle and equidistance, the rotor has a first groove which connects the centre bore with any one of a plurality of holes, a second groove which connects any two adjacent holes in a plurality of holes, a third groove which connects two holes at any interval in a plurality of holes, a fourth groove which connects two holes at any interval in a plurality of holes, and a fifth groove which connects two holes at any interval in a plurality of holes; the first chromatographic column is connected with the eight holes and the twelve holes; the second chromatographic column is connected with the seven-hole and ten-hole; the third chromatographic column is connected with the five holes and the six holes; the fourth chromatographic column is connected with one hole and four holes; the fifth chromatographic column is connected with three holes and eleven holes; the combination has at least seven operating states via the column selector valve. Through the regulation and control of the column selection valve rotor, the alternate switching work among chromatographic columns is realized, and options of direct connection and waste liquid discharge are realized.

Description

Column selection valve and chromatographic column combined device

Technical Field

The utility model relates to a column selection valve and chromatographic column combined device for liquid chromatogram, fluid transmission and control.

Background

The liquid phase chromatographic instrument separates various substances to be separated by utilizing the difference of affinity capacities such as distribution coefficient, adsorption capacity and the like of the substances in two phases, and then analyzes the identified or prepared instrument. In performing a particular experimental run, it is sometimes necessary to switch between multiple columns. The utility model patent of patent number ZL20821840581.X discloses a sampling valve and column selector valve ally oneself with chromatography device, has solved the coexistence problem of a plurality of chromatographic columns, realizes the work of switching over in turn between the chromatographic column, need not manual round trip to change the chromatographic column. However, this device also has the following drawbacks: two column selection valves are needed, and the connection between a certain chromatographic column and the respective column selection holes on the two column selection valves must follow a certain arrangement rule which is generally controlled by software and is difficult to change after being determined; when switching between chromatographic columns or between chromatographic columns and direct connection is carried out, software needs to control two column selection valve rotors to rotate simultaneously, so that respective column selection grooves are communicated with corresponding column selection holes/direct connection holes almost simultaneously, the process has higher requirements on synchronism and accuracy, the software program is complex, and the fault tolerance rate is low.

Disclosure of Invention

In order to solve the above problems, an object of the present invention is to provide a column selection valve and a chromatography column combination device.

In order to achieve the above purpose, the utility model discloses a technical scheme is: a column selection valve and chromatographic column combined device comprises

The column selection valve comprises a stator and a rotor which are mutually and rotatably connected, the stator is provided with a central hole positioned on the rotating axis of the rotor and a plurality of holes which are positioned on the rotating axis of the rotor, are equiangularly and equidistantly distributed by taking the rotating axis of the rotor as the center, the central hole is a mobile phase inlet, the plurality of holes comprise a hole, two holes, three holes, four holes, five holes, six holes, seven holes, eight holes, nine holes, ten holes, eleven holes and twelve holes which are sequentially distributed, the two holes are liquid outlets, the nine holes are waste liquid outlets, the rotor is provided with a first groove capable of communicating the central hole with any one of the plurality of holes, a second groove capable of communicating any two adjacent holes of the plurality of holes, a third groove capable of communicating two holes at any interval of the plurality of holes, a fourth groove capable of communicating two holes at any interval of the plurality of holes, and a fifth groove capable of communicating two holes at any interval of the plurality of holes;

the two ends of the first chromatographic column are respectively connected with the eight holes and the twelve holes;

the two ends of the second chromatographic column are respectively connected with the seven holes and the ten holes;

the two ends of the third chromatographic column are respectively connected with the five holes and the six holes;

a fourth chromatographic column, both ends of which are respectively connected with the first hole and the fourth hole;

a fifth chromatographic column, both ends of which are respectively connected with three holes and eleven holes;

the coupling device has at least seven working states through the rotation of a rotor of the column selection valve, when the coupling device is in a first working state, the central hole is communicated with the two holes through the first groove, and the two ends of the first chromatographic column, the two ends of the second chromatographic column, the two ends of the third chromatographic column, the two ends of the fourth chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the combined device is in a second working state, the central hole is communicated with the nine holes through the first groove, and the two ends of the first chromatographic column, the two ends of the second chromatographic column, the two ends of the third chromatographic column, the two ends of the fourth chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the coupling device is in a third working state, the central hole is communicated with the eight holes through the first groove, the twelve holes are communicated with the two holes through the fifth groove, and the two ends of the second chromatographic column, the two ends of the third chromatographic column, the two ends of the fourth chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the combined device is in a fourth working state, the central hole is communicated with the seven holes through the first groove, the ten holes are communicated with the two holes through the fourth groove, and the two ends of the first chromatographic column, the two ends of the third chromatographic column, the two ends of the fourth chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the coupling device is in a fifth working state, the central hole is communicated with the six holes through the first groove, the five holes are communicated with the two holes through the third groove, and the two ends of the first chromatographic column, the two ends of the second chromatographic column, the two ends of the fourth chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the combined device is in a sixth working state, the central hole is communicated with the four holes through the first groove, the first hole is communicated with the second hole through the second groove, and the two ends of the first chromatographic column, the two ends of the second chromatographic column, the two ends of the third chromatographic column and the two ends of the fifth chromatographic column are blocked with the central hole and the two holes; when the combined device is in a seventh working state, the central hole is communicated with the three holes through the first groove, the eleven holes are communicated with the two holes through the third groove, and the two ends of the first chromatographic column, the two ends of the second chromatographic column, the two ends of the third chromatographic column and the two ends of the fourth chromatographic column are blocked with the central hole and the two holes.

Further, when the combined device is in the first working state, the second groove is communicated with eleven holes and twelve holes, the third groove is communicated with one hole and ten holes, the fourth groove is communicated with five holes and nine holes, and the fifth groove is communicated with six holes and eight holes; when the combined device is in a second working state, the second groove is communicated with the six holes and the seven holes, the third groove is communicated with the five holes and the eight holes, the fourth groove is communicated with the four holes and the twelve holes, and the fifth groove is communicated with the first hole and the three holes; when the coupling device is in a third working state, the second groove is communicated with the five holes and the six holes, the third groove is communicated with the four holes and the seven holes, and the fourth groove is communicated with the three holes and the eleven holes; when the combined device is in a fourth working state, the second groove is communicated with the four holes and the five holes, the third groove is communicated with the three holes and the six holes, and the fifth groove is communicated with the first hole and the eleven holes; when the coupling device is in a fifth working state, the second groove is communicated with the three holes and the four holes, the fourth groove is communicated with the first hole and the nine holes, and the fifth groove is communicated with the ten holes and the twelve holes; when the combined device is in a sixth working state, the third groove is communicated with the three holes and the twelve holes, the fourth groove is communicated with the seven holes and the eleven holes, and the fifth groove is communicated with the eight holes and the ten holes; when the combined device is in a seventh working state, the second groove is communicated with the first hole and the second hole, the fourth groove is communicated with the sixth hole and the tenth hole, and the fifth groove is communicated with the seventh hole and the ninth hole.

Further, when the coupling device is in the first working state, the angle of the rotor is 0 °, the coupling device is switched to the second working state by rotating the rotor counterclockwise by 150 °, switched to the third working state by rotating the rotor counterclockwise by 180 °, switched to the fourth working state by rotating the rotor counterclockwise by 210 °, switched to the fifth working state by rotating the rotor counterclockwise by 240 °, and switched to the sixth working state by rotating the rotor counterclockwise by 300 °; and the seventh working state is switched by rotating the rotor by 330 degrees anticlockwise.

Furthermore, the first hole, the second hole, the third hole, the fourth hole, the fifth hole, the sixth hole, the seventh hole, the eighth hole, the ninth hole, the tenth hole, the eleventh hole and the twelfth hole are distributed in sequence around the clockwise direction.

Furthermore, the first groove, the second groove, the third groove, the fourth groove and the fifth groove are all straight lines, the second groove and the third groove are parallel and are located on one side of the first groove, and the fourth groove and the fifth groove are parallel and are located on the other side of the first groove.

Furthermore, the extension line of the first slot intersects with the fourth slot, and the extension line of the first slot intersects with the fifth slot at one end of the fifth slot.

Further, the length of the first slot is equal to the distance from the central hole to one hole, the length of the second slot is equal to the distance between two adjacent holes, the length of the third slot is equal to the distance between two holes that are two holes apart, the length of the fourth slot is equal to the distance between two holes that are three holes apart, and the length of the fifth slot is equal to the distance between two holes that are one hole apart.

Further, the rotor of the column selector valve is controlled to rotate by a software program.

By adopting the technical scheme, the chromatographic column and column selection valve combined device adopts a single column selection valve, can be connected with at most five chromatographic columns, and realizes alternate switching work among the chromatographic columns and options of direct connection and waste liquid discharge by adjusting and controlling a rotor of the column selection valve; the connection rule of the chromatographic column and the hole on the column selection valve is simple and fixed, the software program is simple and clear, errors are not easy to occur, and the operation is convenient.

Drawings

Fig. 1 is a schematic structural diagram of a combined chromatographic column and column selection valve device according to an embodiment of the present invention, which is in a first operating state;

FIG. 2 is a schematic diagram of the arrangement of the column of FIG. 1 in a second operating state in combination with a column selection valve;

FIG. 3 is a schematic diagram of the column and column selection valve combination of FIG. 1 in a third operating state;

FIG. 4 is a schematic diagram of the column and column selection valve combination of FIG. 1 in a fourth operating state;

FIG. 5 is a schematic diagram of the column and column selection valve combination of FIG. 1 in a fifth operating state;

FIG. 6 is a schematic diagram of the column and column selection valve combination of FIG. 1 in a sixth operating state;

FIG. 7 is a schematic diagram of the column and column selection valve combination of FIG. 1 in a seventh operating state;

FIG. 8 is a schematic front view of a stator of a column selection valve in the apparatus for combining a chromatography column and a column selection valve shown in FIG. 1;

FIG. 9 is a schematic view of the reverse construction of the stator shown in FIG. 8;

FIG. 10 is a schematic diagram of the configuration of the rotor of the column selection valve in the apparatus for combining a chromatography column and a column selection valve shown in FIG. 1.

The reference numbers in the figures are:

10. a sample injection valve; 11. a stator; 110. a central bore; 111. a hole; 112. two holes; 113. three holes; 114. four holes; 115. five holes are formed; 116. six holes; 117. seven holes; 118. eight holes; 119. nine holes; 1110. ten holes; 1111. eleven holes; 1112. twelve holes; 12. a rotor; 121. a first groove; 122. a second groove; 123. a third groove; 124. a fourth groove; 125. a fifth groove;

20. a first chromatographic column;

30. a second chromatography column;

40. a third chromatographic column;

50. a fourth chromatographic column;

60. a fifth chromatographic column.

Detailed Description

The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, enables those skilled in the art to more readily understand the advantages and features of the present invention.

As can be seen from the schematic structural diagrams of fig. 1 to 10, the column selection valve and chromatography column combination device in this embodiment includes a column selection valve 10, a first chromatography column 20, a second chromatography column 30, a third chromatography column 40, and a fourth chromatography column 50.

The column select valve 10 includes a stator 11 and a rotor 12 rotatably connected to each other. The rotor 12 in this embodiment is controlled to rotate by a software program.

The stator 11 has a center hole 110 located on the rotation axis of the rotor 12, and a plurality of holes equally angularly and equidistantly distributed around the rotation axis of the rotor 12, and the plurality of holes includes a first hole 111, a second hole 112, a third hole 113, a fourth hole 114, a fifth hole 115, a sixth hole 116, a seventh hole 117, an eighth hole 118, a ninth hole 119, a tenth hole 1110, an eleventh hole 1111, and a twelfth hole 1112, which are sequentially distributed. Specifically, the first hole 111, the second hole 112, the third hole 113, the fourth hole 114, the fifth hole 115, the sixth hole 116, the seventh hole 117, the eighth hole 118, the ninth hole 119, the tenth hole 1110, the eleventh hole 1111, and the twelfth hole 1112 are sequentially distributed around the clockwise direction.

The central bore 110 is a mobile phase inlet, typically connected to a sample introduction module. Of the plurality of wells, two wells 112 are outlet ports and are typically connected to a collection module, nine wells 119 are waste discharge ports, and the remaining wells are used for connection to a chromatography column. Specifically, the first column 20 has two ends respectively connected to eight wells 118 and twelve wells 1112, the second column 30 has two ends respectively connected to seven wells 117 and ten wells 1110, the third column 40 has two ends respectively connected to five wells 115 and six wells 116, the fourth column 50 has two ends respectively connected to one well 111 and four wells 114, and the fifth column 60 has two ends respectively connected to three wells 113 and eleven wells 1111.

The rotor 12 has a first slot 121, a second slot 122, a third slot 123, a fourth slot 124, and a fifth slot 125. The first groove 121 can communicate the central hole 110 with any one of the plurality of holes, and preferably, the first groove 121 is linear and has a length equal to a distance from the central hole 110 to one hole. The second slot 122 can communicate any adjacent two of the plurality of holes, and preferably, the second slot 122 is linear and has a length equal to the distance between the two adjacent holes. The third groove 123 is capable of communicating two of any two spaced apart holes of the plurality of holes, and preferably, the third groove 123 is linear and has a length equal to the distance between the two spaced apart holes. The fourth slot 124 is capable of communicating two of the plurality of holes, which are spaced apart by any three, and preferably the fourth slot 124 is linear and has a length equal to the distance between two of the plurality of holes, which are spaced apart by three. The fifth groove 125 can communicate two holes of any one of a plurality of holes, and preferably, the fifth groove 125 is linear and has a length equal to the distance between two holes spaced one hole apart. More specifically, the second slot 122 and the third slot 123 are parallel and located at one side of the first slot 121, the fourth slot 124 and the fifth slot 125 are parallel and located at the other side of the first slot 121, an extension line of the first slot 121 intersects with the fourth slot 124, and an extension line of the first slot 121 intersects with the fifth slot 125 and intersects with one end of the fifth slot 125.

The combination has at least seven operating states by rotation of the rotor 12 of the column selector valve 10.

The coupling device has at least six working states through the rotation of the rotor 12 of the column selection valve 10, when the coupling device is in the first working state, the central hole 110 and the two holes 112 are communicated through the first groove 121, the two ends of the first chromatographic column 20, the two ends of the second chromatographic column 30, the two ends of the third chromatographic column 40, the two ends of the fourth chromatographic column 50 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the eleven holes 1111 and the twelve holes 1112, the third groove 123 is communicated with the one holes 111 and the ten holes 1110, the fourth groove 124 is communicated with the five holes 115 and the nine holes 119, and the fifth groove 125 is communicated with the six holes 116 and the eight holes 118;

when the combined device is in the second working state, the central hole 110 and the nine holes 119 are communicated through the first groove 121, the two ends of the first chromatographic column 20, the two ends of the second chromatographic column 30, the two ends of the third chromatographic column 40, the two ends of the fourth chromatographic column 50 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the six holes 116 and the seven holes 117, the third groove 123 is communicated with the five holes 115 and the eight holes 118, the fourth groove 124 is communicated with the four holes 114 and the twelve holes 1112, and the fifth groove 125 is communicated with the one hole 111 and the three holes 113;

when the combined device is in the third working state, the central hole 110 and the eight holes 118 are communicated through the first groove 121, the twelve holes 1112 and the two holes 112 are communicated through the fifth groove 125, the two ends of the second chromatographic column 30, the three ends of the third chromatographic column 40, the two ends of the fourth chromatographic column 50 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the five holes 115 and the six holes 116, the third groove 123 is communicated with the four holes 114 and the seven holes 117, and the fourth groove 124 is communicated with the three holes 113 and the eleven holes 1111;

when the combined device is in the fourth working state, the central hole 110 and the seven hole 117 are communicated through the first groove 121, the ten holes 1110 and the two holes 112 are communicated through the fourth groove 124, the two ends of the first chromatographic column 20, the three ends of the third chromatographic column 40, the two ends of the fourth chromatographic column 50 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the four holes 114 and the five holes 115, the third groove 123 is communicated with the three holes 113 and the six holes 116, and the fifth groove 125 is communicated with the one hole 111 and the eleven holes 1111;

when the combined device is in a fifth working state, the central hole 110 is communicated with the six holes 116 through the first groove 121, the five holes 115 are communicated with the two holes 112 through the third groove 123, the two ends of the first chromatographic column 20, the two ends of the second chromatographic column 30, the two ends of the fourth chromatographic column 50 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the three holes 113 and the four holes 114, the fourth groove 124 is communicated with the one hole 111 and the nine holes 119, and the fifth groove 125 is communicated with the ten holes 1110 and the twelve holes 1112;

when the combined device is in a sixth working state, the central hole 110 is communicated with the four holes 114 through the first groove 121, the first hole 111 is communicated with the second hole 112 through the second groove 122, the two ends of the first chromatographic column 20, the two ends of the second chromatographic column 30, the two ends of the third chromatographic column 40 and the two ends of the fifth chromatographic column 60 are blocked from the central hole 110 and the second hole 112, specifically, the third groove 123 is communicated with the three holes 113 and the twelve holes 1112, the fourth groove 124 is communicated with the seven holes 117 and the eleven holes 1111, and the fifth groove 125 is communicated with the eight holes 118 and the ten holes 1110;

when the combined device is in the seventh working state, the central hole 110 and the three holes 113 are communicated through the first groove 121, the eleven holes 1111 and the two holes 112 are communicated through the third groove 123, both ends of the first chromatographic column 20, both ends of the second chromatographic column 30, both ends of the third chromatographic column 40 and both ends of the fourth chromatographic column 50 are blocked from the central hole 110 and the two holes 112, specifically, the second groove 122 is communicated with the first hole 111 and the twelve holes 1112, the fourth groove 124 is communicated with the six holes 116 and the ten holes 1110, and the fifth groove 125 is communicated with the seven holes 117 and the nine holes 119.

When the coupling device is in the first working state, the angle of the rotor 12 is 0 °, the coupling device is switched to the second working state by rotating the rotor 12 counterclockwise by 150 °, switched to the third working state by rotating the rotor 12 counterclockwise by 180 °, switched to the fourth working state by rotating the rotor 12 counterclockwise by 210 °, switched to the fifth working state by rotating the rotor 12 counterclockwise by 240 °, and switched to the sixth working state by rotating the rotor 12 counterclockwise by 300 °; switching to the seventh operating state takes place by a counterclockwise rotation of the rotor 12 of 330 °.

The combination device in this embodiment is generally used for testing a set of multiple sample sequences, and can also be used for testing a single-kind multi-component sample. The working principle is as follows:

as shown in fig. 1, when the combination device is in the first working state, the sample or reagent enters from the central hole 110 and flows out from the two holes 112 as the liquid outlets, so as to realize the direct connection function; when the rotor 12 rotates anticlockwise to the position shown in fig. 2, the combined device is switched to a second working state, and at the moment, a sample or a reagent enters from the central hole 110 and flows out from the nine holes 119 serving as waste liquid discharge ports, so that a waste liquid discharge function is realized; when the combined device is switched to the third working state shown in fig. 3, the sample or reagent enters from the central hole 110, enters the first chromatographic column 20 from the eight holes 118, passes through the twelve holes 1112, and flows out from the two holes 112; when the combined device is switched to the fourth operating state shown in fig. 4, the sample or reagent enters the second chromatographic column 30 through the central hole 110 and the seven holes 117, passes through the ten holes 1110, and flows out of the two holes 112; when the combination device is switched to the fifth operating state shown in FIG. 5, the sample or reagent enters the third chromatographic column 40 through the central hole 110 and the six holes 116, passes through the five holes 115, and flows out of the two holes 112; when the combined device is switched to the sixth working state shown in fig. 6, the sample or reagent enters the fourth chromatographic column 50 through the central hole 110 and the four holes 114, passes through the first hole 111, and flows out of the second hole 112; when the combination device is switched to the seventh operating state shown in fig. 7, the sample or reagent enters the fourth column 50 through the central hole 110, the three holes 113, the eleven holes 1111, and the two holes 112.

In the above, the rotation angle of the rotor 12 is controlled by software, so that alternate switching work among different chromatographic columns and options of direct connection and waste liquid discharge are realized; each chromatographic column can realize forward sample loading and detection in turn, and basically has no interference with each other; meanwhile, the system also supports the forward cleaning function for each chromatographic column. The arrows in fig. 1 to 7 indicate the flow direction of the sample or reagent.

The device for combining the chromatographic columns and the column selection valve in the embodiment adopts a single column selection valve, can be connected with at most four chromatographic columns, and realizes alternate switching work among the chromatographic columns and options of direct connection and waste liquid discharge by regulating and controlling the rotor of the column selection valve; the connection rule of the chromatographic column and the hole of the column selection valve is simple and fixed, the software program is simple and clear, errors are not easy to occur, and the operation is convenient.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose of the embodiments is to enable people skilled in the art to understand the contents of the present invention and to implement the present invention, which cannot limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.

Claims (8)

1. A column selection valve and chromatographic column combined device is characterized in that: it comprises

A column selection valve (10) comprising a stator (11) and a rotor (12) rotatably connected to each other, the stator (11) having a center hole (110) located on a rotation axis of the rotor (12), a plurality of holes equally angularly and equidistantly distributed centering on the rotation axis of the rotor (12), the center hole (110) being a mobile phase inlet, the plurality of holes including a hole (111), a hole (112), a hole (113), a hole (114), a hole (115), a hole (116), a hole (117), a hole (118), a hole (119), a hole (1110), a hole (1111), and a hole (1112) sequentially distributed, the hole (112) being a liquid outlet, the hole (119) being a waste liquid discharge port, the rotor (12) having a first groove (121) and a hole (1112) capable of communicating the center hole (110) with any one of the plurality of holes, A second groove (122) capable of communicating any two adjacent ones of the plurality of holes, a third groove (123) capable of communicating two holes of the plurality of holes arbitrarily spaced apart by two, a fourth groove (124) capable of communicating two holes of the plurality of holes arbitrarily spaced apart by three, and a fifth groove (125) capable of communicating two holes of the plurality of holes arbitrarily spaced apart by one;

a first chromatographic column (20) having both ends connected to the eight holes (118) and the twelve holes (1112), respectively;

a second chromatographic column (30) having both ends connected to the seven-well (117) and the ten-well (1110), respectively;

a third chromatographic column (40) having two ends respectively connected to the five holes (115) and the six holes (116);

a fourth chromatographic column (50) having two ends respectively connected to the first well (111) and the fourth well (114);

a fifth chromatography column (60) having both ends connected to the three-well (113) and the eleven-well (1111), respectively;

the combination device has at least seven working states through the rotation of a rotor (12) of the column selection valve (10), when the combination device is in a first working state, the central hole (110) and the two holes (112) are communicated through the first groove (121), and the two ends of the first chromatographic column (20), the two ends of the second chromatographic column (30), the two ends of the third chromatographic column (40), the two ends of the fourth chromatographic column (50) and the two ends of the fifth chromatographic column (60) are blocked with the central hole (110) and the two holes (112); when the combined device is in a second working state, the central hole (110) is communicated with the nine holes (119) through the first groove (121), and the two ends of the first chromatographic column (20), the two ends of the second chromatographic column (30), the two ends of the third chromatographic column (40), the two ends of the fourth chromatographic column (50) and the two ends of the fifth chromatographic column (60) are blocked from the central hole (110) and the two holes (112); when the combined device is in a third working state, the central hole (110) and the eight holes (118) are communicated through the first groove (121), the twelve holes (1112) and the two holes (112) are communicated through the fifth groove (125), and two ends of the second chromatographic column (30), two ends of the third chromatographic column (40), two ends of the fourth chromatographic column (50) and two ends of the fifth chromatographic column (60) are blocked with the central hole (110) and the two holes (112); when the combined device is in a fourth working state, the central hole (110) and the seven holes (117) are communicated through the first groove (121), the ten holes (1110) and the two holes (112) are communicated through the fourth groove (124), and two ends of the first chromatographic column (20), two ends of the third chromatographic column (40), two ends of the fourth chromatographic column (50) and two ends of the fifth chromatographic column (60) are blocked from the central hole (110) and the two holes (112); when the combined device is in a fifth working state, the central hole (110) is communicated with the six holes (116) through the first groove (121), the five holes (115) are communicated with the two holes (112) through the third groove (123), and two ends of the first chromatographic column (20), two ends of the second chromatographic column (30), two ends of the fourth chromatographic column (50) and two ends of the fifth chromatographic column (60) are blocked with the central hole (110) and the two holes (112); when the combined device is in a sixth working state, the central hole (110) is communicated with the four holes (114) through the first groove (121), the first hole (111) is communicated with the second hole (112) through the second groove (122), and the two ends of the first chromatographic column (20), the two ends of the second chromatographic column (30), the two ends of the third chromatographic column (40) and the two ends of the fifth chromatographic column (60) are blocked from the central hole (110) and the two holes (112); when the combined device is in a seventh working state, the central hole (110) and the three holes (113) are communicated through the first groove (121), the eleven holes (1111) and the two holes (112) are communicated through the third groove (123), and both ends of the first chromatographic column (20), both ends of the second chromatographic column (30), both ends of the third chromatographic column (40) and both ends of the fourth chromatographic column (50) are blocked from the central hole (110) and the two holes (112).

2. The column selection valve in combination with a chromatography column of claim 1, wherein: when the combined device is in a first working state, the second groove (122) communicates with the eleventh hole (1111) and the twelfth hole (1112), the third groove (123) communicates with the first hole (111) and the tenth hole (1110), the fourth groove (124) communicates with the fifth hole (115) and the ninth hole (119), and the fifth groove (125) communicates with the sixth hole (116) and the eighth hole (118); when the combined device is in the second working condition, the second slot (122) communicates between the six (116) and seven (117) orifices, the third slot (123) communicates between the five (115) and eight (118) orifices, the fourth slot (124) communicates between the four (114) and twelve (1112) orifices, and the fifth slot (125) communicates between the first (111) and three (113) orifices; when the combined device is in a third working condition, the second slot (122) communicates the five hole (115) and the six hole (116), the third slot (123) communicates the four hole (114) and the seven hole (117), and the fourth slot (124) communicates the three hole (113) and the eleven hole (1111); when the combined device is in a fourth working condition, the second slot (122) communicates between the four holes (114) and the five holes (115), the third slot (123) communicates between the three holes (113) and the six holes (116), and the fifth slot (125) communicates between the one hole (111) and the eleven hole (1111); when the combined device is in a fifth work condition, the second slot (122) communicates between the three holes (113) and the four holes (114), the fourth slot (124) communicates between the first hole (111) and the nine holes (119), and the fifth slot (125) communicates between the ten holes (1110) and the twelve holes (1112); when the combined device is in a sixth working condition, the third slot (123) communicates between the three holes (113) and the twelve holes (1112), the fourth slot (124) communicates between the seven holes (117) and the eleven holes (1111), and the fifth slot (125) communicates between the eight holes (118) and the ten holes (1110); when the combined device is in a seventh working state, the second groove (122) is communicated with the first hole (111) and the twelve holes (1112), the fourth groove (124) is communicated with the six holes (116) and the ten holes (1110), and the fifth groove (125) is communicated with the seven holes (117) and the nine holes (119).

3. The column selection valve in combination with a chromatography column of claim 1, wherein: when the coupling device is in a first working state, the angle of the rotor (12) is 0 degrees, the coupling device is switched to a second working state through the counterclockwise rotation of the rotor (12) by 150 degrees, is switched to a third working state through the counterclockwise rotation of the rotor (12) by 180 degrees, is switched to a fourth working state through the counterclockwise rotation of the rotor (12) by 210 degrees, is switched to a fifth working state through the counterclockwise rotation of the rotor (12) by 240 degrees, and is switched to a sixth working state through the counterclockwise rotation of the rotor (12) by 300 degrees; switching to a seventh operating state takes place by rotating the rotor (12) by 330 ° in the counterclockwise direction.

4. The column selection valve in combination with a chromatography column of claim 1, wherein: the one hole (111), the two holes (112), the three holes (113), the four holes (114), the five holes (115), the six holes (116), the seven holes (117), the eight holes (118), the nine holes (119), the ten holes (1110), the eleven holes (1111), and the twelve holes (1112) are sequentially distributed around a clockwise direction.

5. The column selection valve in combination with a chromatography column of claim 1, wherein: the first groove (121), the second groove (122), the third groove (123), the fourth groove (124) and the fifth groove (125) are all linear, the second groove (122) and the third groove (123) are parallel and are positioned on one side of the first groove (121), and the fourth groove (124) and the fifth groove (125) are parallel and are positioned on the other side of the first groove (121).

6. The column selection valve in combination with a chromatography column of claim 5, wherein: an extension line of the first groove (121) intersects the fourth groove (124), and the extension line of the first groove (121) intersects the fifth groove (125) at one end of the fifth groove (125).

7. The column selection valve in combination with a chromatography column of claim 1, wherein: the first slot (121) has a length equal to the distance from the central aperture (110) to one of the apertures, the second slot (122) has a length equal to the distance between two adjacent ones of the apertures, the third slot (123) has a length equal to the distance between two of the apertures that are spaced apart by two of the apertures, the fourth slot (124) has a length equal to the distance between two of the apertures that are spaced apart by three of the apertures, and the fifth slot (125) has a length equal to the distance between two of the apertures that are spaced apart by one of the apertures.

8. The column selection valve in combination with a chromatography column of claim 1, wherein: the rotor (12) of the column selector valve (10) is controlled in rotation by a software program.

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