CN212829873U

CN212829873U - Reagent storage device for continuously and uniformly mixing reagent magnetic beads

Info

Publication number: CN212829873U
Application number: CN202021196228.XU
Authority: CN
Inventors: 刘海健; 李坚
Original assignee: Shanghai Saihan Biotechnology Co ltd
Current assignee: Shanghai Saihan Biotechnology Co ltd
Priority date: 2020-06-24
Filing date: 2020-06-24
Publication date: 2021-03-30
Anticipated expiration: 2030-06-24

Abstract

The utility model discloses a reagent magnetic bead lasts reagent storage device of mixing, including cylindric insulation box and the refrigeration subassembly that has the refrigerator, be equipped with the annular tray on the lower extreme lateral wall in refrigeration chamber, be equipped with the rotary disk on the annular tray, the center end of rotary disk is equipped with the transmission shaft that stretches into electric intracavity, the bottom side in refrigeration chamber is installed to the refrigerator, refrigeration subassembly sets up in electric intracavity, the warp bearing is equipped with a plurality of axis of rotation on the rotary disk, the top of axis of rotation is equipped with rotatory tray, every the avris of rotatory tray is equipped with a plurality of vertical ascending bounding walls, encloses into the chamber of placing the reagent bottle between a plurality of bounding walls, the bottom lateral wall in refrigeration chamber is equipped with the ring gear, every the bottom of axis of rotation is equipped with the drive gear with the ring gear meshing. The utility model discloses can rotate the mixing in the middle of the cold-stored process of reagent, avoid reagent to take place to deposit and make the reuse in later stage not convenient for differently of concentration.

Description

Reagent storage device for continuously and uniformly mixing reagent magnetic beads

Technical Field

The utility model relates to the technical field of medical equipment, specifically be a reagent magnetic bead lasts reagent storage device of mixing.

Background

At present, the research of exosome extraction mainly focuses on extracting exosomes from cell and serum samples, and the extraction methods mainly adopted by the research mainly include: the method comprises an ultrahigh-speed centrifugation method, a filtration centrifugation method, a density gradient ultrahigh-speed centrifugation method, an immunomagnetic bead combined ultrahigh-speed centrifugation method and a chromatography method, wherein reagent magnetic beads need to be applied in the immunomagnetic bead combined ultrahigh-speed centrifugation method, the existing reagent magnetic beads are refrigerated by a refrigerating device, and precipitation is not easy to occur for a long time, so that the density distribution is not uniform, the subsequent use is influenced, and manual shaking is needed.

SUMMERY OF THE UTILITY MODEL

For solving the defect that prior art exists, the utility model provides a reagent magnetic bead lasts reagent storage device of mixing.

In order to solve the technical problem, the utility model provides a following technical scheme:

the utility model relates to a reagent storage device for continuously and uniformly mixing reagent and magnetic beads, which comprises a cylindrical heat preservation box body and a refrigeration assembly with a refrigerator, wherein the top of the heat preservation box body is provided with a top cover, the heat preservation box body is internally provided with a refrigeration cavity and an electric cavity which are vertically arranged and are mutually independent, the lower end side wall of the refrigeration cavity is provided with an annular supporting block, the annular supporting block is provided with a rotary disk, the central end of the rotary disk is provided with a transmission shaft extending into the electric cavity, the refrigerator is arranged at the bottom side of the refrigeration cavity, the refrigeration assembly is arranged in the electric cavity, the rotary disk is provided with a plurality of rotating shafts through a bearing, the top end of each rotating shaft is provided with a rotating supporting block, the side of each rotating supporting block is provided with a plurality of vertical upward surrounding plates, a placing cavity for placing reagent bottles is formed by the surrounding plates, the bottom end side wall of the refrigeration cavity, a transmission gear meshed with the gear ring is arranged at the bottom end of each rotating shaft, a driving motor is arranged in the electric cavity, and an output shaft of the driving motor is in transmission connection with the shaft end of the transmission shaft; an opening is formed in the heat preservation box body, and a cover door is arranged at the opening.

As an optimized technical scheme of the utility model, be equipped with a plurality of first ventilation holes on the rotary disk.

As an optimized technical scheme of the utility model, be equipped with first rubber buffer layer on the lateral wall of bounding wall.

As an optimized technical scheme of the utility model, a plurality of balls are inlayed to the upper surface of annular tray.

As an optimal technical scheme of the utility model, be equipped with the second ventilation hole of densely covered on the rotatory tray, and the upper surface of rotatory tray is equipped with second rubber buffer layer.

The utility model has the advantages that: this kind of reagent magnetic bead lasts reagent storage device of mixing, insert the reagent magnetic bead solution bottle that needs the storage when using between the bounding wall on the rotatory layer board, the chamber of placing that utilizes a plurality of bounding walls to constitute comes to carry on spacingly and accomodate the solution bottle, wherein under refrigeration effect of refrigeration subassembly, utilize the refrigerator to come to refrigerate the refrigeration chamber, driving motor drives the rotary disk simultaneously and rotates, the pivoted rotary disk then makes the drive gear of axis of rotation bottom rotate along the ring gear, and it rotates to drive the axis of rotation, the axis of rotation then drives rotatory tray and rotates, and then drive the solution bottle and carry out the rotation and rotate along the rotary disk, thereby played the reagent magnetic bead that lasts the mixing in the solution bottle, make the reagent magnetic bead in the storage insulation box can not take place the sediment, make things convenient for following along taking along with in later stage.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic structural diagram of a reagent storage device for continuously mixing reagent magnetic beads according to the present invention;

FIG. 2 is a schematic diagram of the internal structure of the incubator of the reagent storage apparatus for continuously mixing the reagent and magnetic beads according to the present invention;

fig. 3 is a schematic structural diagram of the ball of the reagent storage device of the present invention for continuously and uniformly mixing the reagent magnetic beads.

In the figure: 1. a heat preservation box body; 2. a refrigeration assembly; 3. a refrigerator; 4. a top cover; 5. a refrigerated chamber; 6. an electrical cavity; 7. an annular supporting block; 8. rotating the disc; 9. a drive shaft; 10. a rotating shaft; 11. rotating the supporting block; 12. enclosing plates; 13. a ring gear; 14. a transmission gear; 15. a drive motor; 16. an opening; 17. covering the door; 18. a first vent hole; 19. a first rubber cushion layer; 20. a ball bearing; 21. a second vent hole; 22. and the second rubber buffer layer.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

Example (b): as shown in fig. 1, fig. 2 and fig. 2, the reagent storage device for continuously mixing reagent and magnetic beads of the present invention comprises a cylindrical heat preservation box 1 and a refrigeration assembly 2 with a refrigerator 3, wherein a top cover 4 is arranged on the top of the heat preservation box 1, a refrigeration cavity 5 and an electrical cavity 6 which are arranged up and down and are independent from each other are arranged inside the heat preservation box 1, an annular supporting block 7 is arranged on the side wall of the lower end of the refrigeration cavity 5, a rotary disk 8 is arranged on the annular supporting block 7, a transmission shaft 9 extending into the electrical cavity 6 is arranged at the central end of the rotary disk 8, the refrigerator 3 is arranged at the bottom side of the refrigeration cavity 5, the refrigeration assembly 2 is arranged in the electrical cavity 6, a plurality of rotation shafts 10 are arranged on the rotary disk 8 through bearings, a rotation supporting block 11 is arranged at the top end of the rotation shaft 10, a plurality of vertical upward enclosing plates 12 are arranged at the side of each rotation supporting block, a placing cavity for placing the reagent bottles is defined by the enclosing plates 12, a gear ring 13 is arranged on the side wall of the bottom end of the refrigerating cavity 5, a transmission gear 14 meshed with the gear ring 13 is arranged at the bottom end of each rotating shaft 10, a driving motor 15 is arranged in the electric cavity 6, and an output shaft of the driving motor 15 is in transmission connection with the shaft end of the transmission shaft 9; an opening 16 is arranged on the heat preservation box body 1, a cover door 17 is arranged at the opening 16, when in use, a reagent magnetic bead solution bottle to be stored is inserted between the coamings 12 on the rotating splint, a placing cavity formed by a plurality of coamings 12 is used for limiting and containing the solution bottle, wherein under the refrigeration action of the refrigeration component 2, the refrigeration cavity is refrigerated by the refrigerator 3, meanwhile, the driving motor 15 drives the rotating disc 8 to rotate, the rotating disc 8 enables the transmission gear 14 at the bottom end of the rotating shaft 10 to rotate along the gear ring 13, and drives the rotating shaft 10 to rotate, the rotating shaft 10 drives the rotating support block 11 to rotate, thereby driving the solution bottle to rotate and rotate along the rotating disk 8, thus continuously and uniformly mixing the reagent magnetic beads in the solution bottle, so that the reagent magnetic beads in the storage and heat preservation box body 1 can not be precipitated, and the reagent magnetic beads in the later period can be conveniently taken along with the use.

Wherein, a plurality of first ventilation holes 18 are arranged on the rotary disk 8, and cold air generated by the surface and the refrigerator 3 enters the upper part of the refrigerating chamber 5.

The outer side wall of the enclosing plate 12 is provided with a first rubber buffer layer 19, so that the effect of buffer protection is achieved.

A plurality of balls 20 are embedded in the upper surface of the annular supporting block 7, and friction between the annular supporting block 7 and the rotating disc 8 is reduced.

The rotary supporting block 11 is provided with the second ventilation holes 21 which are densely distributed, so that the contact area of the rotary supporting block 11 and cold air in the cold storage cavity 5 is increased, a better cold storage effect is achieved, the upper surface of the rotary supporting block 11 is provided with the second rubber buffer layer 22, a protection effect is achieved, and rigid collision between the solution bottle and the rotary supporting block 11 is avoided.

The utility model discloses insert the reagent magnetic bead solution bottle that will need the storage when using between the bounding wall 12 on the rotatory layer board, the chamber of placing that utilizes a plurality of bounding walls 12 to constitute carries on spacingly and accomodates the solution bottle, wherein under the refrigeration of refrigeration subassembly 2, utilize refrigerator 3 to come to refrigerate the chamber, driving motor 15 drives rotary disk 8 simultaneously rotates, pivoted rotary disk 8 makes the drive gear 14 that makes axis of rotation 10 bottom rotate along ring gear 13, and drive axis of rotation 10 and rotate, axis of rotation 10 then drives rotatory tray 11 and rotates, and then drive the solution bottle and carry out the rotation and rotate along rotary disk 8, thereby played the reagent magnetic bead that lasts the mixing in the solution bottle, make the reagent magnetic bead in storage insulation box 1 can not take place to deposit, make things convenient for following along with taking in later stage and use.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a reagent storage device of continuous mixing of reagent magnetic bead, includes cylindric insulation box (1) and has refrigeration subassembly (2) of refrigerator (3), the top of insulation box (1) is equipped with top cap (4), its characterized in that: the inside of heat preservation box body (1) is equipped with and is set up from top to bottom and mutually independent cold storage chamber (5) and electric chamber (6), be equipped with annular tray (7) on the lower extreme lateral wall in cold storage chamber (5), be equipped with rotary disk (8) on annular tray (7), the center end of rotary disk (8) is equipped with transmission shaft (9) that stretch into in electric chamber (6), install the bottom side at cold storage chamber (5) refrigerator (3), refrigeration component (2) set up in electric chamber (6), the warp bearing is equipped with a plurality of axis of rotation (10) on rotary disk (8), the top of axis of rotation (10) is equipped with rotatory tray (11), every the avris of rotatory tray (11) is equipped with a plurality of vertical ascending bounding wall (12), encloses into placing the chamber of placing the reagent bottle between a plurality of bounding wall (12), the bottom lateral wall in cold storage chamber (5) is equipped with and is equipped with ring gear (13), a transmission gear (14) meshed with the gear ring (13) is arranged at the bottom end of each rotating shaft (10), a driving motor (15) is arranged in the electric cavity (6), and an output shaft of the driving motor (15) is in transmission connection with the shaft end of the transmission shaft (9); an opening (16) is arranged on the heat preservation box body (1), and a cover door (17) is arranged at the opening (16).

2. The device for storing the reagent, which is used for continuously mixing the magnetic beads of the reagent, according to claim 1, wherein a plurality of first ventilation holes (18) are formed on the rotating disk (8).

3. The device for storing the reagent, which is used for continuously mixing the reagent and the magnetic bead, according to claim 1, is characterized in that a first rubber buffer layer (19) is arranged on the outer side wall of the enclosing plate (12).

4. The device for storing the reagent, which is used for continuously and uniformly mixing the reagent and the magnetic bead, according to claim 1, is characterized in that a plurality of balls (20) are embedded in the upper surface of the annular supporting block (7).

5. The device for storing the reagent and the magnetic bead for mixing the reagent and the magnetic bead continuously as claimed in claim 1, wherein the second ventilation holes (21) are densely distributed on the rotating support block (11), and the second rubber buffer layer (22) is arranged on the upper surface of the rotating support block (11).