CN113650923A - Kit based on development of CAR-T cells - Google Patents
Kit based on development of CAR-T cells Download PDFInfo
- Publication number
- CN113650923A CN113650923A CN202110802749.8A CN202110802749A CN113650923A CN 113650923 A CN113650923 A CN 113650923A CN 202110802749 A CN202110802749 A CN 202110802749A CN 113650923 A CN113650923 A CN 113650923A
- Authority
- CN
- China
- Prior art keywords
- test tube
- ring
- vibration
- reagent box
- car
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011161 development Methods 0.000 title claims description 5
- 238000012360 testing method Methods 0.000 claims abstract description 102
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 97
- 230000011712 cell development Effects 0.000 claims abstract description 10
- 230000005540 biological transmission Effects 0.000 claims description 34
- 230000000694 effects Effects 0.000 claims description 16
- 238000005057 refrigeration Methods 0.000 claims description 7
- 230000018109 developmental process Effects 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 29
- 238000003825 pressing Methods 0.000 description 31
- 238000005096 rolling process Methods 0.000 description 12
- 230000010355 oscillation Effects 0.000 description 8
- 238000009423 ventilation Methods 0.000 description 7
- 238000007789 sealing Methods 0.000 description 6
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 238000004378 air conditioning Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000031146 intracellular signal transduction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003534 oscillatory effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kit developed based on CAR-T cells, and relates to the technical field of cell development. The test tube vibrating rack assembly can enable the test tube to rotate at a high speed automatically when rotating along with the rotary vibrating carrier plate, the reagent liquid in the test tube is in a vortex flowing state, the test tube can vibrate when rotating through the vibrating carrier assembly, the reagent liquid in the test tube can be subjected to vortex vibration shaking, the shaking-up working efficiency of the reagent liquid can be improved, and the mixing reaction quality of the reagent liquid can be improved.
Description
Technical Field
The invention relates to the technical field of cell development, and particularly relates to a kit developed based on CAR-T cells.
Background
CAR-T cell is called chimeric antigen receptor medically and is composed of extracellular antigen binding region composed of light chain (VL) and heavy chain (VH) from monoclonal antibody, middle part composed of flexible hinge region to form single chain antibody, transmembrane region and intracellular signal transduction region.
In the prior art, the reagent kit has a single structural function, and cannot perform vortex oscillation shaking treatment on the reagent solution in the test tube only by shaking the test tube to shake up the reagent solution, so that the shaking work efficiency of the reagent solution is low, the reagent solution cannot be rapidly and sufficiently mixed, and the reaction quality of the reagent solution is influenced, so that the reagent kit developed based on CAR-T cells is disclosed to meet the requirements of people.
Disclosure of Invention
The invention aims to provide a kit developed based on CAR-T cells, and aims to solve the problems that in the background technology, the reagent solution shaking is low in working efficiency and cannot be quickly and fully mixed to influence the reaction quality of the reagent solution due to the fact that the kit is single in structural function and only shakes the reagent solution uniformly by shaking a test tube, and the reagent solution in the test tube cannot be subjected to vortex oscillation shaking uniformly, so that the reagent solution can be quickly and fully mixed, and the mixing reaction quality of the reagent solution is improved.
In order to achieve the above purpose, the present application provides the following technical solutions: the utility model provides a kit based on CAR-T cell development, includes the reagent box body, the inside of reagent box body is provided with the vibration carrier subassembly that is used for loading and drives the test tube oscillation, the outside of vibration carrier subassembly is provided with drive assembly, the upper end of vibration carrier subassembly is provided with the pressure rack subassembly, the vibration carrier subassembly including shake soon and carry dish and fixed mounting in the vibrating motor who shakes soon and carry the dish bottom side, the upper end that shakes soon carries the dish rotates through connecting axle a and is connected with test tube year seat, the inside bottom fixed mounting of reagent box body has brake motor, brake motor is used for the drive shake soon and carry the dish to rotate, shake soon and carry the dish to rotate and utilize simultaneously drive assembly drives test tube year seat is rotated by oneself.
Based on the structure, the test tube loaded with reagent solution is placed on the test tube carrier, the test tube on the test tube carrier is compressed and positioned by the pressing frame assembly, the rotary vibration carrier is driven by the brake motor to rotate, meanwhile, the test tube carrier can be driven by the transmission component to rotate automatically, so that the test tube rotates along the central point of the rotary vibration carrier along with the rotary vibration carrier, and the test tube which is compressed and positioned on the test tube carrier seat can automatically rotate at high speed to promote the reagent liquid in the test tube to be in a vortex flow state, the test tube on the test tube carrier seat can vibrate when rotating and rotating at high speed through the vibration carrier assembly, so that the reagent liquid in the test tube is continuously oscillated and can be subjected to vortex oscillation and even shaking treatment, thereby be favorable to improving the even work efficiency that shakes of reagent liquid in the test tube for reagent liquid in the test tube can quick intensive mixing.
Preferably, a brake rotating shaft is fixedly mounted at the driving end of the brake motor, an assembly sliding groove is formed in the surface of the upper end of the brake rotating shaft, a movable sliding rod is fixedly mounted on the surface of the bottom end of the rotary vibration carrying disc, the movable sliding rod is connected with the assembly sliding groove in a sliding mode, a vibration spring is fixedly mounted between the rotary vibration carrying disc and the brake rotating shaft, and the vibration spring is movably sleeved on the outer side of the movable sliding rod.
Furthermore, through setting up the movable slide bar, vibrating spring and assembly spout, the movable slide bar mutually supports with the assembly spout, make the load dish that shakes soon can carry out spacing removal from top to bottom on the braking spiral shaft, braking motor drive braking spiral shaft rotates, drive the load dish that shakes soon and rotate when braking spiral shaft 15 rotates, vibrating spring takes place to compress when the load dish that shakes soon moves down on the braking spiral shaft, the elasticity that produces when utilizing the vibrating spring compression can play the load dish that shakes soon and move up on the braking spiral shaft, use vibrating motor can drive the load dish that shakes soon and carry out continuous vibration on the braking spiral shaft.
Preferably, the transmission assembly comprises a transmission gear ring and a limiting ring fixedly mounted at the bottom end of the transmission gear ring, a limiting slide block is fixedly mounted on the side surface of the limiting ring, a limiting slide groove is formed in the inner side surface of the reagent box body, and the limiting slide block is connected with the limiting slide groove in a sliding mode.
Furthermore, through setting up spacing spout and spacing spout, spacing slider and spacing spout are mutually supported for spacing slip about spacing ring can be internal at the kit, has reached and has made transmission ring gear and spacing ring not take place pivoted purpose.
Preferably, the test tube carries the seat and is annular equidistance interval distribution in the dish upper end is carried in the spiral vibration, the fixed surface of connecting axle a installs driven gear, the transmission ring gear meshes with driven gear mutually.
Furthermore, through setting up driven gear, the transmission ring gear does not take place to rotate with spacing ring, has reached and to have made the rotatory test tube that can drive when carrying the dish rotation of shaking soon and has carried the seat purpose of rotating by oneself.
Preferably, the circumferential side surface of the rotary vibration carrying disc is rotatably connected with a limiting roller wheel through a connecting mounting frame, the inner side surface of the limiting ring is provided with a limiting rolling groove, and the limiting roller wheel is in rolling connection with the limiting rolling groove.
Further, through setting up spacing gyro wheel and spacing slot rolling, spacing gyro wheel and spacing slot rolling mutually support for the dish that carries that shakes soon can carry out spacing rotation in spacing ring inboard, and spacing ring and transmission ring gear can follow the dish synchronous oscillation that carries that shakes soon.
Preferably, the pressing frame assembly comprises a positioning pressing ring and a positioning pressing block connected with the bottom side of the positioning pressing ring in a rotating mode through a connecting shaft b, the positioning pressing block is distributed on the bottom side of the positioning pressing ring at equal intervals in an annular mode, the rotary vibration carrying disc is connected with a positioning screw rod in a rotating mode through a connecting seat, the surface of the rotary vibration carrying disc is far away from one side of the positioning screw rod and is fixedly provided with a guide straight rod, the guide straight rod penetrates through the surface of the positioning pressing ring in a movable mode, and the positioning screw rod is in threaded connection with the positioning pressing ring.
Further, through setting up positioning screw, direction straight-bar, location clamping ring and location clamping ring, positioning screw can drive the activity of location clamping ring when rotating, uses the positioning screw forward rotation to drive the location clamping ring and upwards move about, uses the positioning screw reverse rotation to drive the location clamping ring and carries out activity downwards, has reached and has utilized the location briquetting to compress tightly the purpose of location with the test tube on the test tube year seat.
Preferably, the diameter of the driven gear is smaller than the diameter of the transmission gear ring.
Further, through setting up like this, can drive driven gear high-speed when the transmission ring gear rotates and rotate, reached and make the rotatory test tube that shakes carry the seat and carry out the purpose that rotates by oneself at a relatively high speed of carrying the dish when rotatory.
Preferably, the outside of reagent box body is provided with the refrigeration subassembly, the refrigeration subassembly include the refrigerator body and fixed mounting in the cover that ventilates of reagent box body inside wall, the refrigerator body fixed mounting in the lateral surface of reagent box body, the gas outlet has been seted up at the surface interval of the cover that ventilates.
Further, through setting up refrigeration subassembly, use the refrigerator body can be with producing in air conditioning discharges to the reagent box body through the gas outlet of ventilating the cover for air conditioning can distribute in arranging to the reagent box body, guarantees the refrigeration effect in the reagent box body, prevents that reagent box body inside temperature from influencing reagent liquid activity easily when too high.
Preferably, the fixed surface of reagent box body installs the control processor, the fixed surface of control processor installs control panel, control panel's output with the input electric connection of control processor, the output of control processor with the equal electric connection of input of brake motor, vibrating motor and refrigerator body.
Furthermore, by arranging the control processor and the control panel, the brake motor, the vibration motor and the refrigerator body can be controlled to start and stop through the control panel, so that the refrigerator is more convenient to use and operate.
Preferably, the upper end activity of reagent box body articulates there is sealed lid, the one end fixed mounting of sealed lid has the hasp, the one end fixed mounting of reagent box body has the locking groove.
Furthermore, through setting up hasp and locking groove, reached the reagent box body of being convenient for go up open with the purpose of airtight sealed lid, be convenient for seal the reagent box body.
In conclusion, the technical effects and advantages of the invention are as follows:
1. the test tube loading seat is provided with a test tube loading seat, the test tube loading seat is provided with a pressing frame assembly, the pressing frame assembly is used for pressing and positioning the test tube on the test tube loading seat, a brake motor is used for driving a rotary vibration carrying disc to rotate, meanwhile, the transmission assembly can drive the test tube loading seat to rotate automatically, so that the test tube rotates along the central point of the rotary vibration carrying disc along with the rotary vibration carrying disc, the test tube pressed and positioned on the test tube loading seat can rotate at high speed automatically, the reagent liquid in the test tube is promoted to be in a vortex flowing state, the test tube on the test tube loading seat can vibrate when rotating and rotating at high speed through the vibration carrying frame assembly, the reagent liquid in the test tube is continuously oscillated, the reagent liquid in the test tube can be oscillated and shaken uniformly, the oscillating working efficiency of the reagent liquid in the test tube can be improved, and the reagent liquid in the test tube can be mixed sufficiently and rapidly, the quality of the mixed reaction of the reagent solution is improved;
2. according to the invention, the refrigerator body, the ventilation cover and the air outlet are arranged, so that cold air generated by using the refrigerator body can be discharged into the reagent box body through the ventilation cover, the activity of reagent liquid is prevented from being easily influenced when the temperature in the reagent box body is too high, and the storage effect of the reagent liquid is favorably ensured;
3. according to the invention, the limiting idler wheels and the limiting rolling grooves are arranged, so that the rotary vibration carrying disc can perform limiting rotation on the inner side of the limiting ring, the limiting ring can synchronously vibrate along with the rotary vibration carrying disc, the limiting sliding block is in sliding connection with the limiting sliding grooves, so that the limiting ring can perform limiting sliding up and down in the kit body, and the purpose of preventing the transmission gear ring and the limiting ring from rotating is achieved;
4. the positioning screw rod can drive the positioning compression ring to move, and the positioning pressing block can be used for tightly pressing and positioning the test tube on the test tube carrier seat, so that the test tube is effectively prevented from falling off the test tube carrier seat, the test tube is prevented from being easily damaged, and the use reliability is improved;
5. according to the invention, the control panel and the control processor are arranged, the output end of the control panel is electrically connected with the input end of the control processor, the output end of the control processor is electrically connected with the input ends of the brake motor, the vibration motor and the refrigerator body, the control information of the control panel is transmitted into the control processor, and the control processor transmits the information to the brake motor, the vibration motor and the refrigerator body, so that the purpose of controlling the brake motor, the vibration motor and the refrigerator body to start and stop through the control panel is achieved, and the use and operation are more convenient.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic cross-sectional view of a reagent cartridge of the present invention;
FIG. 3 is a schematic perspective view of the oscillating carriage assembly and the pressing frame assembly of the present invention;
FIG. 4 is a schematic front view of the oscillating carriage assembly and the clamping frame assembly of the present invention;
FIG. 5 is a perspective view of the transmission assembly of the present invention;
FIG. 6 is a partial cross-sectional structural schematic of the present invention;
FIG. 7 is an enlarged view of portion A of FIG. 6;
fig. 8 is an enlarged structural view of a portion B in fig. 6.
In the figure: 1. a reagent cartridge body; 11. sealing the box cover; 12. a control processor; 13. a limiting chute; 14. braking the motor; 15. braking the rotary shaft; 16. assembling a chute; 2. vibrating the carrier assembly; 21. a rotational vibration carrying disk; 211. a movable slide bar; 212. a vibration spring; 22. a connecting shaft a; 23. a test tube carrier; 24. connecting and mounting the frame; 25. limiting the idler wheel; 26. a driven gear; 27. positioning a screw rod; 28. a guide straight rod; 29. a vibration motor; 3. a transmission assembly; 31. a transmission gear ring; 32. a limiting ring; 33. limiting rolling grooves; 34. a limiting slide block; 4. a pressing frame assembly; 41. positioning the compression ring; 42. a connecting shaft b; 43. positioning a pressing block; 5. a refrigeration assembly; 51. a refrigerator body; 52. a ventilation hood; 53. and an air outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b): referring to fig. 1-8, a CAR-T cell based development kit includes a reagent cartridge 1, and the reagent cartridge 1 may be any one of the kits in the prior art.
Based on the structure, the test tube loaded with reagent liquid is placed on the test tube carrier seat 23, the test tube on the test tube carrier seat 23 is compressed and positioned by the pressing frame assembly 4, the rotary vibration carrier disc 21 is driven to rotate by the brake motor 14, meanwhile, the transmission assembly 3 can drive the test tube carrier seat 23 to rotate by itself, so that the test tube rotates along the central point of the rotary vibration carrier disc 21 along with the rotary vibration carrier disc 21, the test tube compressed and positioned on the test tube carrier seat 23 can rotate at high speed by itself, so that the reagent liquid in the test tube is in a vortex flow state, the test tube on the test tube carrier seat 23 can vibrate when rotating and rotating at high speed by the vibration carrier frame assembly 2, the reagent liquid in the test tube can generate continuous oscillation, the reagent liquid in the test tube can be subjected to vortex oscillation and uniform shaking treatment, and the shaking work efficiency of the reagent liquid in the test tube can be improved, so that the reagent liquid in the test tube can be quickly and fully mixed.
As a preferred implementation manner in this embodiment, a brake rotating shaft 15 is fixedly installed at a driving end of a brake motor 14, an assembly sliding slot 16 is formed on an upper end surface of the brake rotating shaft 15, a movable sliding rod 211 is fixedly installed at a bottom end surface of a rotational vibration carrying disc 21, the movable sliding rod 211 is slidably connected with the assembly sliding slot 16, a vibration spring 212 is fixedly installed between the rotational vibration carrying disc 21 and the brake rotating shaft 15, the vibration spring 212 is movably sleeved outside the movable sliding rod 211, the movable sliding rod 211 and the assembly sliding slot 16 are mutually matched, so that the rotational vibration carrying disc 21 can perform an up-and-down limiting movement on the brake rotating shaft 15, the brake motor 14 drives the brake rotating shaft 15 to rotate, the rotational vibration carrying disc 21 is driven to rotate when the brake rotating shaft 15 rotates, the vibration spring 212 is compressed when the rotational vibration carrying disc 21 moves down on the brake rotating shaft 15, and an elastic force generated when the vibration spring 212 is compressed can bounce the rotational vibration carrying disc 21 to move up on the brake rotating shaft 15, the use of the vibration motor 29 enables the rotary vibration plate 21 to continuously vibrate on the brake rotor shaft 15.
As a preferred implementation manner in this embodiment, as shown in fig. 5 and fig. 6, the transmission assembly 3 includes a transmission toothed ring 31 and a limiting circular ring 32 fixedly mounted at the bottom end of the transmission toothed ring 31, a limiting slide block 34 is fixedly mounted on a side surface of the limiting circular ring 32, a limiting slide groove 13 is formed in the inner side surface of the reagent box body 1, the limiting slide block 34 is slidably connected with the limiting slide groove 13, and the limiting slide block 34 and the limiting slide groove 13 are matched with each other, so that the limiting circular ring 32 can perform vertical limiting sliding in the reagent box body 1, and the purpose of preventing the transmission toothed ring 31 and the limiting circular ring 32 from rotating is achieved.
As a preferred embodiment in this embodiment, as shown in fig. 3, the test tube holders 23 are distributed on the upper end of the rotational vibration plate 21 at equal intervals in a ring shape, the driven gear 26 is fixedly mounted on the surface of the connecting shaft a22, the transmission gear ring 31 is meshed with the driven gear 26, the transmission gear ring 31 and the limiting ring 32 do not rotate, and the purpose of driving the test tube holders 23 to rotate by themselves when the rotational vibration plate 21 rotates is achieved.
As a preferred implementation manner in this embodiment, the circumferential side surface of the rotational vibration carrying disc 21 is rotatably connected with a limiting roller 25 through the connecting mounting frame 24, the inner side surface of the limiting ring 32 is provided with a limiting rolling groove 33, the limiting roller 25 is in rolling connection with the limiting rolling groove 33, the limiting roller 25 and the limiting rolling groove 33 are mutually matched, so that the rotational vibration carrying disc 21 can perform limiting rotation inside the limiting ring 32, and the limiting ring 32 and the transmission gear ring 31 can synchronously vibrate along with the rotational vibration carrying disc 21.
As a preferred embodiment in this embodiment, the pressure frame assembly 4 includes a positioning pressing ring 41 and a positioning pressing block 43 rotatably connected to the bottom side of the positioning pressing ring 41 through a connecting shaft b42, the positioning pressing block 43 is annularly and equidistantly distributed on the bottom side of the positioning pressing ring 41, the rotary vibration disc 21 is rotatably connected to the positioning screw 27 through a connecting seat, a guiding straight rod 28 is fixedly mounted on one side of the surface of the rotary vibration disc 21 away from the positioning screw 27, the guiding straight rod 28 movably penetrates through the surface of the positioning pressing ring 41, the positioning screw 27 is in threaded connection with the positioning pressing ring 41, the positioning screw 27 can drive the positioning pressing ring 41 to move when rotating, the positioning pressing ring 41 can be driven to move upwards by forward rotation of the positioning screw 27, the positioning pressing ring 41 can be driven to move downwards by reverse rotation of the positioning screw 27, and the purpose of pressing and positioning the test tube on the test tube carrier 23 by using the positioning pressing block 43 is achieved.
As a preferred embodiment in this embodiment, the diameter of the driven gear 26 is smaller than the diameter of the transmission gear ring 31, and the transmission gear ring 31 can drive the driven gear 26 to rotate at a high speed when rotating, so as to achieve the purpose of driving the test tube holder 23 to rotate at a relatively high speed when the rotary vibration plate 21 rotates.
As a preferred embodiment in this embodiment, as shown in fig. 2, a refrigerating assembly 5 is disposed on the outer side of the reagent box 1, the refrigerating assembly 5 includes a refrigerating device 51 and a ventilation cover 52 fixedly mounted on the inner side wall of the reagent box 1, the refrigerating device 51 is fixedly mounted on the outer side surface of the reagent box 1, the ventilation cover 52 is disposed on the surface of the air outlet 53 at a distance, and the generated cold air can be discharged into the reagent box 1 through the air outlet 53 of the ventilation cover 52 by using the refrigerating device 51, so that the cold air can be distributed and discharged into the reagent box 1, thereby ensuring the refrigerating effect in the reagent box 1 and preventing the activity of the reagent solution from being easily affected when the temperature inside the reagent box 1 is too high.
As a preferred embodiment in this embodiment, a control processor 12 is fixedly mounted on the surface of the reagent cartridge 1, a control panel is fixedly mounted on the surface of the control processor 12, an output end of the control panel is electrically connected with an input end of the control processor 12, an output end of the control processor 12 is electrically connected with input ends of the brake motor 14, the vibration motor 29 and the refrigerator body 51, control information of the control panel is transmitted into the control processor 12, and the control processor 12 transmits information to the brake motor 14, the vibration motor 29 and the refrigerator body 51, so that the purpose of controlling the brake motor 14, the vibration motor 29 and the refrigerator body 51 to start and stop through the control panel is achieved, and the use and operation are more convenient.
As a preferred implementation manner in this embodiment, as shown in fig. 1, the upper end of the reagent box body 1 is movably hinged with a sealing box cover 11, one end of the sealing box cover 11 is fixedly provided with a lock catch, one end of the reagent box body 1 is fixedly provided with a locking groove, the lock catch and the locking groove are matched with each other, when the sealing box cover 11 is sealed on the reagent box body 1, the lock catch is buckled in the locking groove, so as to achieve the purpose of facilitating the opening and sealing of the sealing box cover 11 on the reagent box body 1.
The working principle of the invention is as follows:
in the using process of the kit, a test tube filled with reagent liquid is placed on the test tube carrier seat 23, the test tube on the test tube carrier seat 23 is compressed and positioned by the pressing frame assembly 4, the rotary vibration carrier disc 21 is driven to rotate by the brake motor 14, meanwhile, the test tube carrier seat 23 can be driven to rotate by the transmission assembly 3, so that the test tube rotates along the central point of the rotary vibration carrier disc 21 along with the rotary vibration carrier disc 21, the test tube compressed and positioned on the test tube carrier seat 23 can rotate at high speed by itself, the reagent liquid in the test tube is enabled to be in a vortex flow state, the test tube on the test tube carrier seat 23 can vibrate when rotating and rotating at high speed by the vibration carrier frame assembly 2, the reagent liquid in the test tube can continuously oscillate, the reagent liquid in the test tube can be subjected to vortex oscillation and shaking uniformly, and the shaking work efficiency of the reagent liquid in the test tube can be improved, so that the reagent liquid in the test tube can be quickly and fully mixed.
The refrigerator body 51 can discharge the generated cold air into the reagent box body 1 through the ventilation cover 52, the reagent liquid activity is prevented from being easily influenced when the temperature in the reagent box body 1 is overhigh, the start and stop of the braking motor 14, the vibration motor 29 and the refrigerator body 51 can be controlled through the control panel, the use and the operation are more convenient, the limiting idler wheel 25 and the limiting rolling groove 33 are arranged, the rotary vibration loading disc 21 can rotate in a limiting way on the inner side of the limiting circular ring 32, the limiting circular ring 32 can synchronously vibrate along with the rotary vibration loading disc 21, the output end of the control panel is electrically connected with the input end of the control processor 12, the output end of the control processor 12 is electrically connected with the input ends of the braking motor 14, the vibration motor 29 and the refrigerator body 51, the control information of the control panel is transmitted into the control processor 12, and the control processor 12 transmits the information to the braking motor 14, the vibration motor 29 and the refrigerator body 51, the purpose of controlling the starting and stopping of the brake motor 14, the vibration motor 29 and the refrigerator body 51 through the control panel is achieved, and the use and the operation are more convenient.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (10)
1. A kit developed based on CAR-T cells, comprising a reagent cartridge body (1), characterized in that: a vibration carrier assembly (2) used for loading and driving the test tube to oscillate is arranged in the reagent box body (1), a transmission assembly (3) is arranged on the outer side of the vibration carrier assembly (2), a pressure rack assembly (4) is arranged at the upper end of the vibration carrier assembly (2), the vibration carrier assembly (2) comprises a rotary vibration carrier disc (21) and a vibration motor (29) fixedly arranged at the bottom side of the rotary vibration carrier disc (21), the upper end of the rotary vibration carrying disc (21) is rotationally connected with a test tube carrying seat (23) through a connecting shaft a (22), a brake motor (14) is fixedly arranged at the bottom end inside the reagent box body (1), the brake motor (14) is used for driving the rotary vibration carrying disc (21) to rotate, the rotary vibration carrying disc (21) rotates and simultaneously drives the test tube carrying seat (23) to rotate by itself by utilizing the transmission assembly (3).
2. A CAR-T cell development based kit according to claim 1, characterized in that: the drive end fixed mounting of brake motor (14) has braking spiral shaft (15), assembly spout (16) have been seted up to the upper end surface of braking spiral shaft (15), the bottom fixed surface of the dish (21) of shaking soon installs movable slide bar (211), activity slide bar (211) and assembly spout (16) sliding connection, fixed mounting has vibrating spring (212) between dish (21) of shaking soon and braking spiral shaft (15), vibrating spring (212) activity cup joint in the activity slide bar (211) outside.
3. A CAR-T cell development based kit according to claim 1, characterized in that: the reagent box is characterized in that the transmission assembly (3) comprises a transmission gear ring (31) and a limiting ring (32) fixedly installed at the bottom end of the transmission gear ring (31), a limiting slide block (34) is fixedly installed on the side face of the limiting ring (32), a limiting slide groove (13) is formed in the inner side surface of the reagent box body (1), and the limiting slide block (34) is connected with the limiting slide groove (13) in a sliding mode.
4. A CAR-T cell based development kit according to claim 2 or 3, characterized in that: test tube year seat (23) are annular equidistance interval distribution in carry dish (21) upper end of shaking soon, the fixed surface of connecting axle a (22) installs driven gear (26), transmission ring gear (31) and driven gear (26) mesh mutually.
5. A CAR-T cell based development kit according to claim 1 or 3, characterized in that: the side of week of spin-vibration year dish (21) rotates through connecting dress frame (24) and is connected with spacing gyro wheel (25), spacing roll groove (33) have been seted up to the internal side surface of spacing ring (32), spacing gyro wheel (25) and spacing roll groove (33) roll connection.
6. A CAR-T cell development based kit according to claim 1, characterized in that: the upper end of vibration carrier subassembly (2) is provided with pressure frame subassembly (4), pressure frame subassembly (4) including location clamping ring (41) with location clamping ring (41) bottom side is through connecting axle b (42) location briquetting (43) of rotating the connection, location briquetting (43) be annular equidistance interval distribution in location clamping ring (41) bottom side, it is connected with positioning screw (27) to shake through the connecting seat rotation soon year dish (21), the surface of shaking soon year dish (21) is kept away from one side fixed mounting of positioning screw (27) has direction straight-bar (28), direction straight-bar (28) activity run through in location clamping ring (41) surface, positioning screw (27) and location clamping ring (41) threaded connection.
7. A CAR-T cell development based kit according to claim 4, characterized in that: the diameter of the driven gear (26) is smaller than the diameter of the transmission gear ring (31).
8. A CAR-T cell development based kit according to claim 1, characterized in that: the outside of reagent box body (1) is provided with refrigeration subassembly (5), refrigeration subassembly (5) including refrigerator body (51) and fixed mounting in ventilate cover (52) of reagent box body (1) inside wall, refrigerator body (51) fixed mounting in the lateral surface of reagent box body (1), gas outlet (53) have been seted up at the surface interval of ventilating cover (52).
9. A CAR-T cell development based kit according to claim 1 or 8, characterized in that: the fixed surface of reagent box body (1) installs control processor (12), the fixed surface of control processor (12) installs control panel, control panel's output with the input electric connection of control processor (12), the output of control processor (12) with the equal electric connection of input of brake motor (14), vibrating motor (29) and refrigerator body (51).
10. A CAR-T cell development based kit according to claim 1, characterized in that: the utility model discloses a reagent box, including reagent box body (1), one end fixed mounting has the hasp, the upper end activity of reagent box body (1) articulates there is sealed lid (11), the one end fixed mounting of sealed lid (11) has the locking groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110802749.8A CN113650923A (en) | 2021-07-15 | 2021-07-15 | Kit based on development of CAR-T cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110802749.8A CN113650923A (en) | 2021-07-15 | 2021-07-15 | Kit based on development of CAR-T cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113650923A true CN113650923A (en) | 2021-11-16 |
Family
ID=78489404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110802749.8A Pending CN113650923A (en) | 2021-07-15 | 2021-07-15 | Kit based on development of CAR-T cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113650923A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6450681B1 (en) * | 1998-12-17 | 2002-09-17 | Aventis Behring Gmbh | Process for dissolving albumin flakes in a liquid and arrangement for carrying out the process |
| CN205868133U (en) * | 2016-06-27 | 2017-01-11 | 深圳市亚辉龙生物科技股份有限公司 | Reagent mixing device |
| CN106904354A (en) * | 2017-04-17 | 2017-06-30 | 杜美玲 | It is a kind of with the blood test reagent pipe receiver for shaking up function |
| CN107497346A (en) * | 2017-08-22 | 2017-12-22 | 浙江省海洋水产研究所 | Pre-treatment experimental provision for biological sample petrochina hydrocarbon content parameter |
| CN208894105U (en) * | 2018-06-07 | 2019-05-24 | 张书源 | A kind of high school student's chemical experiment reagent oscillation device |
| CN209530717U (en) * | 2019-01-18 | 2019-10-25 | 杭州谱可检测技术有限公司 | A kind of chemical detection test tube rocking equipment |
| CN209771972U (en) * | 2019-03-13 | 2019-12-13 | 大连美纳医学检验实验室有限公司 | Oscillator for sample preparation system in biotechnology laboratory |
| CN210486267U (en) * | 2019-07-25 | 2020-05-08 | 福建和泽生物科技有限公司 | Frozen serum thawing device |
| CN111498254A (en) * | 2020-04-24 | 2020-08-07 | 上海康与生物科技有限公司 | Gene amplification detection kit |
| CN111842290A (en) * | 2020-07-20 | 2020-10-30 | 王金波 | A wash maintenance device for machine parts |
| CN212829873U (en) * | 2020-06-24 | 2021-03-30 | 上海赛罕生物技术有限公司 | Reagent storage device for continuously and uniformly mixing reagent magnetic beads |
| CN112718655A (en) * | 2020-12-16 | 2021-04-30 | 王焯 | Workpiece cleaning equipment for mechanical production |
-
2021
- 2021-07-15 CN CN202110802749.8A patent/CN113650923A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6450681B1 (en) * | 1998-12-17 | 2002-09-17 | Aventis Behring Gmbh | Process for dissolving albumin flakes in a liquid and arrangement for carrying out the process |
| CN205868133U (en) * | 2016-06-27 | 2017-01-11 | 深圳市亚辉龙生物科技股份有限公司 | Reagent mixing device |
| CN106904354A (en) * | 2017-04-17 | 2017-06-30 | 杜美玲 | It is a kind of with the blood test reagent pipe receiver for shaking up function |
| CN107497346A (en) * | 2017-08-22 | 2017-12-22 | 浙江省海洋水产研究所 | Pre-treatment experimental provision for biological sample petrochina hydrocarbon content parameter |
| CN208894105U (en) * | 2018-06-07 | 2019-05-24 | 张书源 | A kind of high school student's chemical experiment reagent oscillation device |
| CN209530717U (en) * | 2019-01-18 | 2019-10-25 | 杭州谱可检测技术有限公司 | A kind of chemical detection test tube rocking equipment |
| CN209771972U (en) * | 2019-03-13 | 2019-12-13 | 大连美纳医学检验实验室有限公司 | Oscillator for sample preparation system in biotechnology laboratory |
| CN210486267U (en) * | 2019-07-25 | 2020-05-08 | 福建和泽生物科技有限公司 | Frozen serum thawing device |
| CN111498254A (en) * | 2020-04-24 | 2020-08-07 | 上海康与生物科技有限公司 | Gene amplification detection kit |
| CN212829873U (en) * | 2020-06-24 | 2021-03-30 | 上海赛罕生物技术有限公司 | Reagent storage device for continuously and uniformly mixing reagent magnetic beads |
| CN111842290A (en) * | 2020-07-20 | 2020-10-30 | 王金波 | A wash maintenance device for machine parts |
| CN112718655A (en) * | 2020-12-16 | 2021-04-30 | 王焯 | Workpiece cleaning equipment for mechanical production |
Non-Patent Citations (1)
| Title |
|---|
| 柳建敏等: "一种具有混匀功能试剂盒的设计", 《中国医疗设备》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207478479U (en) | One kind waves blender | |
| CN110743412A (en) | Flour detection equipment capable of quantitatively feeding by utilizing gravity and automatically stirring | |
| CN110433351A (en) | Oscillating uniform device and blood component separator | |
| CN113650923A (en) | Kit based on development of CAR-T cells | |
| CN114602360B (en) | Blood is platelet constant temperature surge tank for internal medicine | |
| CN113856872A (en) | Desulfurization and denitrification catalyst grinding system and grinding method | |
| CN210229697U (en) | Calcium chloride desiccant agitator tank | |
| CN216538256U (en) | A anti-spillage test tube vibration device that falls for medicine detects | |
| CN108636237A (en) | A kind of planetary efficient mixing equipment | |
| CN110201768A (en) | A kind of roll-turning device for thinning of chemical industry Raw material processing | |
| CN207929123U (en) | A kind of vibrations mixing arrangement of lubricating oil processing | |
| CN208320660U (en) | A kind of automatic rocking equipment of drug | |
| CN216093939U (en) | Formula granulation machine that sways that unloading was collected | |
| CN110522262A (en) | A kind of tablecloth that convenience is replaced automatically | |
| CN109178399A (en) | A kind of smoking apparatus and its application method | |
| CN214211546U (en) | Dust collector in matrimony vine packaging process | |
| CN217016295U (en) | Beef processing is with stainless steel closed type stirring and is mixed material device | |
| CN212943521U (en) | Horizontal spiral filtering centrifuge for processing pleurotus eryngii | |
| CN210787275U (en) | Amino acid granulating device | |
| CN109133250B (en) | Intelligent production auxiliary equipment for powdered activated carbon | |
| CN209076806U (en) | Stirring ball mill | |
| CN220803223U (en) | Nanometer microsphere emulsion synthesizer | |
| CN214126850U (en) | Automatic tea rolling device | |
| CN208550324U (en) | Rotary blush powder box | |
| CN108687495A (en) | Device for supplying workpiece |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211116 |
|
| RJ01 | Rejection of invention patent application after publication |