CN212748931U - Metabonomics metabolite draws kit - Google Patents
Metabonomics metabolite draws kit Download PDFInfo
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- CN212748931U CN212748931U CN202021289443.4U CN202021289443U CN212748931U CN 212748931 U CN212748931 U CN 212748931U CN 202021289443 U CN202021289443 U CN 202021289443U CN 212748931 U CN212748931 U CN 212748931U
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- extraction kit
- collecting plate
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- 239000002207 metabolite Substances 0.000 title claims abstract description 48
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 89
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 42
- 238000001914 filtration Methods 0.000 claims abstract description 30
- 238000000605 extraction Methods 0.000 claims abstract description 29
- 238000000861 blow drying Methods 0.000 claims abstract description 27
- 238000007664 blowing Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims description 16
- 230000003139 buffering effect Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000002705 metabolomic analysis Methods 0.000 claims description 2
- 230000001431 metabolomic effect Effects 0.000 claims description 2
- 238000012031 short term test Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 description 7
- 229910001873 dinitrogen Inorganic materials 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 1
- 210000004880 lymph fluid Anatomy 0.000 description 1
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- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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Abstract
The utility model discloses a metabonomics metabolite draws kit. The metabonomics metabolite extraction kit comprises a collecting plate, a filtering bracket, a replaceable filter tip, a nitrogen blow-drying box and a replaceable nitrogen blow nozzle; the replaceable filter tip is connected with the filtering bracket through threads and then placed on the collecting plate to form a filtering and collecting box, and the replaceable nitrogen blowing nozzle is connected with the nitrogen blow-drying box through threads and then placed on the filtering and collecting box to form a metabolite extraction kit. The utility model discloses a overlap child formula mode, every part all is that the matching is reasonable, and overall operation is simple and convenient for work rate, it is extravagant to avoid unnecessary time, has reached the target of short-term test.
Description
Technical Field
The invention belongs to the field of experimental equipment, and relates to a metabonomics metabolite extraction kit.
Background
Metabonomics is a research mode for carrying out quantitative analysis on all metabolites in an organism and searching the relative relation between the metabolites and physiological and pathological changes by imitating the research ideas of genomics and proteomics, and is a component of system biology. The metabolomics protocol includes: sample collection, sample pretreatment, metabolite detection, data processing and biogenic analysis. The uniformity and the time of the sample pretreatment have great influence on the detection of the metabolites. The traditional sample pretreatment has more defects and inconveniences: including complicated procedures, long treatment time, great influence of human operation factors and poor extraction uniformity of metabolites. The specific program is complicated and is represented as follows: the extraction process of the metabolites needs the processes of metabolite extraction, protein removal and other impurity components removal, nitrogen blow-drying and re-dissolution. The operation of each step requires processing in a separate 1.5ml microcentrifuge tube, and the processing of large samples is limited by instruments in each operation process, so that the sample processing process is repeated and lacks efficiency. The long treatment time is reflected in: the above-mentioned sample treatment time is substantially about 3 hours, and in the case of the treatment of multiple samples, the time required is further increased, which results in an excessively long sample treatment time and serious degradation of metabolites. Human operational factor effects are generally present: the liquid needs to be transferred back and forth during the above procedures, and the contamination or loss caused by these procedures may affect the identification and quantification of the metabolites. In the nitrogen drying process, the liquid splashes to pollute other samples due to the overlarge nitrogen flow rate. The effects of these manual manipulations also affect the accuracy of metabolite detection. The poor uniformity of metabolite extraction is reflected in the reduced metabolite fidelity and reduced homogeneity between samples due to various deficiencies in the sample pretreatment process in the above aspects, and finally results in false positives and false negatives. Because of the limitations, beginners or unskilled experimenters can cause precious human sample pollution or waste and lose manpower, material resources and financial resources when extracting metabolites. There is a great need for an excellent, programmed, standardized metabolite extraction kit to meet the above experimental needs. The applicant creatively and pioneering designs a serum or plasma metabonomics metabolite extraction kit according to years of metabonomics (tissue, blood, cell) metabolite extraction experience.
SUMMERY OF THE UTILITY MODEL
The utility model aims at the above-mentioned not enough of prior art, provide a metabolite extraction kit.
The utility model discloses a following technical scheme of purpose accessible realizes:
a metabolite extraction kit comprises a collecting plate, a filtering bracket, a replaceable filter tip, a nitrogen blow-drying box and a replaceable nitrogen blowing nozzle; wherein the collecting plate is provided with a plurality of cylindrical collecting units; the replaceable filter tip is conical, and outer ring threads are arranged at the bottom of the replaceable filter tip and are connected with the threads of the internal thread holes of the filter support; the lower plate surface of the nitrogen blow-drying box is provided with a plurality of holes with internal threads, the positions of which correspond to the cylindrical collecting units on the collecting plate; the outer ring of the replaceable nitrogen blowing nozzle is threaded and can be in threaded connection with the nitrogen blow-drying box; the replaceable filter tip is connected with the filtering bracket through threads and then placed on the collecting plate to form a filtering and collecting box, and the replaceable nitrogen blowing nozzle is connected with the nitrogen blow-drying box through threads and then placed on the filtering and collecting box to form a metabolite extraction kit.
As a preferred embodiment of the present invention, the collecting plate has a cylindrical collecting unit with an open top and a closed bottom.
As a preferred embodiment of the present invention, the number of the cylindrical collecting units arranged on the collecting plate is 48 to 96, preferably 96.
As an embodiment of the present invention, the collecting plate is integrally rectangular, and the long side surface and the wide side surface are marked with marks respectively.
As a preferred embodiment of the present invention, the filtering support is an appearance of an integral rectangular plate, and the long side surface and the wide side surface are respectively marked with corresponding marks.
As a preferred embodiment of the utility model, the replaceable filter tip is conical, the bottom is provided with outer ring threads and is connected with the internal thread hole threads of the filter support, no outer wall exists from the top 1/3-3/5 sections, the top is connected with the vertebral body by more than two connecting ribs, and the inside of the replaceable filter tip is filled with the filter matrix.
As a preferred embodiment of the present invention, the number of the connecting ribs is 3 or 4.
As an optimal implementation mode of the utility model, nitrogen gas weather the whole cuboid outward appearance of box, the four corners is equipped with the pillar, its right side has 1 buffering air inlet.
Has the advantages that:
the utility model discloses metabolite draws kit can provide unified processing flow according to different sample types such as serum, plasma, urine, cerebrospinal fluid, lymph fluid, saliva, amniotic fluid and bodily fluid samples such as bile, and the suitability is strong, can be used to the detection of multiple sample.
The utility model discloses a overlap child formula mode, every part all is that the matching is reasonable, and overall operation is simple and convenient for work rate, it is extravagant to avoid unnecessary time, has reached the target of short-term test.
The invention adopts the replaceable nitrogen gas blowing sleeve head, and can reduce the possibility of pollution among samples generated in the sample processing process. Further optimizing the processing of the sample and facilitating the purification of the metabolites of the sample.
According to the invention, the radian of the cone is ingeniously utilized to combine with the filtering matrix, so that on one hand, the radian of the cone can fully utilize the filtering area, and the recovery rate of metabolites is remarkably improved; on the other hand, the arc-shaped filtering substrate can increase the contact area of the extraction solvent and the metabolite, and is beneficial to increasing the recovery of the metabolite.
Drawings
FIG. 1 is a schematic diagram of a 96-well collection plate structure
The reference numbers in the figures are: 1 is a collecting plate, 1-1 is a collecting plate with a long surface of 15cm, 1-2 is a collecting plate with a wide surface of 10cm, 1-3 is a collecting plate with a high surface, 1-4 is a cylindrical collecting unit with a diameter of 1cm, 1-5 is a wide edge label, and 1-6 is a long edge label.
FIG. 2 is a schematic view of a filter support and a replaceable filter
The reference numbers in the figures are: 2 is a filtering bracket, 3 is a replaceable filter tip, 2-1 is a long surface of the filtering bracket, 2-2 is a wide surface of the filtering bracket of 10cm, 2-3 is an internal thread hole, 2-4 is a long edge label, 2-5 is a wide edge label,
FIG. 3 is a schematic view of a replaceable filter
3-1 is a bottom external thread opening, 3-2 is a connecting rib, and 3-3 is a polymeric SPE filter substrate.
FIG. 4 is a schematic diagram of a nitrogen blow-drying box and a replaceable nitrogen blowing nozzle:
4 is a nitrogen blow-drying box, 5 is a replaceable nitrogen blowing nozzle, 4-1 is a hollow cuboid box, 4-2 is a nitrogen blow-drying bracket, 4-3 is a hole with an internal thread with the diameter of 1cm, and 4-4 is a buffering air inlet
FIG. 5 is a combined metabolite extraction kit
1 is the collecting plate, 2 is filtering support, 3 is the interchangeable filter tip, 4 is nitrogen gas and weathers the box, and 5 is the interchangeable nitrogen gas blowing nozzle. In order to clearly show the connection relationship of all parts, partial cylindrical collecting units and corresponding holes are omitted in the collecting plate, the filtering bracket and the nitrogen blow-drying box.
Detailed Description
Example 1
A serum or plasma rapid metabolite extraction kit comprises a collecting plate 1, a filtering bracket 2, a replaceable filter tip 3, a nitrogen blow-drying box 4 and a replaceable nitrogen blowing nozzle 5.
As shown in fig. 1, the collecting plate 1 is a rectangular parallelepiped: the length is 15cm, the width is 10cm, the height is 3.5cm, 96 cylindrical collecting units with the diameter of 1cm and the height of 3cm are arranged in the collecting units 1-4, the long side is marked with 1-12 numbers, and the wide side is marked with letters A-H.
As shown in fig. 2, the filter holder 2 is a 96-hole threaded rectangular parallelepiped plate: the length is 15cm, the width is 10cm, the height is 0.5cm, the diameter of each internal threaded hole 2-3 is 1cm, the inner wall of each internal threaded hole is threaded, the number and the positions of the internal threaded holes 2-3 are the same as those of the cylindrical collecting units 1-4 on the collecting plate 1, and the positions of the internal threaded holes correspond to those of the cylindrical collecting units 1-4 on the collecting plate 1. The long side of the filter support 2 is marked with numbers 1-12, and the wide side is marked with letters A-H.
As shown in fig. 3, the replaceable filter 3 is conical: the bottom is an outer ring threaded port 3-1 with the diameter of 0.95cm and is in threaded connection with an inner threaded hole 2-3 of the filtering bracket, the bottom is hollow, the height of a cone is 2.5cm, the section 1cm away from the top end has no outer wall, the top end is connected with a cone body through three connecting ribs 3-2, and the inside of the filtering bracket is filled with an annular filtering substrate 3-3.
As shown in figure 4, the nitrogen blow-drying box is a cuboid, the length of the nitrogen blow-drying box is 18cm, the width of the nitrogen blow-drying box is 13cm, the height of the nitrogen blow-drying box is 1cm, 4-2 nitrogen blow-drying brackets are arranged, the lower plate is provided with 96 holes 4-3 with threaded inner walls, the diameter of each hole is 1cm, the left side of each hole is provided with a buffer air inlet with the diameter of 1cm, and the height of each nitrogen blow-drying bracket.
The replaceable nitrogen blowing nozzle 5 is in a cone shape: the bottom is a hollow shape with the diameter of 0.95cm and the outer ring thread connected with the nitrogen blow-drying box and the height of the cone of 1.5 cm.
Referring to fig. 2, after the replaceable filter tip 3 is connected with the filter support 4 through threads, the replaceable filter tip is placed on a 96-hole collecting plate to form a filter collecting box;
adding 10-30 μ l of sample to the filter substrate, and incubating for 15-20 min;
referring to fig. 4, the replaceable nitrogen blowing nozzle 5 is connected with the nitrogen blow-drying box 3 through a screw thread and then is placed on the filtering and collecting box (fig. 5); the buffer air inlet 4 is connected with nitrogen gas flow to dry for 30 to 45 minutes;
adding 50-150 μ l organic extraction solvent to extract metabolite, shaking at 120rpm for 15-30 min to dissolve metabolite completely;
centrifuging, filtering, and centrifuging at 100 × g for 2 min
The replaceable filter tip and the blowing nozzle are discarded, and the rest can be recycled. The remaining 96-well collection plate was subjected to LC-MS/MS analysis.
The utility model provides a programmed metabolite draws kit is applicable to multiple heterogeneous sample pretreatment. The experimenter can easily operate, and the possibility of multiple treatment pollution among samples is avoided. The conical design is more targeted and can improve the extraction yield of the metabolites. The nesting doll type design increases the simplicity of operation and avoids the possibility of metabolite degradation caused by overlong operation time. The parts not involved in the present invention are the same as or can be implemented using the prior art.
Claims (9)
1. A metabolomics metabolite extraction kit, which is characterized in that: the metabonomics metabolite extraction kit comprises a collecting plate (1), a filtering bracket (2), a replaceable filter tip (3), a nitrogen blow-drying box (4) and a replaceable nitrogen blowing nozzle (5); wherein, the collecting plate (1) is provided with a plurality of cylindrical collecting units (1-4); the filter support (2) is provided with internal thread holes (2-3) the positions and the number of which correspond to those of the cylindrical collecting units (1-4) on the collecting plate (1), the replaceable filter tip (3) is conical, and the bottom of the replaceable filter tip is provided with an outer ring thread (3-1) which is in threaded connection with the internal thread holes (2-3) of the filter support (2); the lower plate surface of the nitrogen blow-drying box (4) is provided with a plurality of holes (4-3) with internal threads, the positions of which correspond to the cylindrical collecting units (1-4) on the collecting plate; the outer ring of the replaceable nitrogen blowing nozzle (5) is threaded and can be in threaded connection with a hole (4-3) of the internal thread of the nitrogen blow-drying cassette; the replaceable filter tip (3) is connected with the internal thread hole (2-3) of the filtering bracket (2) through threads and then is placed on the collecting plate (1) to form a filtering collecting box, and the replaceable nitrogen blowing nozzle (5) is connected with the internal thread hole (4-3) of the nitrogen blow-drying box (4) through threads and then is placed on the filtering collecting box to form the metabonomics metabolite extraction kit.
2. The metabolite extraction kit according to claim 1: the method is characterized in that: the cylindrical collecting units (1-4) arranged on the collecting plate (1) are of a structure with an opening at the top end and a closed structure at the bottom end.
3. The metabolite extraction kit according to claim 1 or 2: the method is characterized in that: the number of the cylindrical collecting units (1-4) arranged on the collecting plate (1) is 48-96.
4. The metabolite extraction kit according to claim 3: the method is characterized in that: the number of the cylindrical collecting units (1-4) arranged on the collecting plate (1) is 96.
5. The metabolite extraction kit according to claim 3: the method is characterized in that: the whole collecting plate (1) is in the appearance of a cuboid, and marks are respectively engraved on the long surface (1-1) and the wide surface (1-2) of the collecting plate.
6. The metabolite extraction kit according to claim 1: the method is characterized in that: the filtering bracket (2) is the appearance of an integral cuboid plate, and the long surface (2-1) and the wide surface (2-2) of the filtering bracket are respectively engraved with corresponding marks.
7. The metabolite extraction kit according to claim 1: the method is characterized in that: the replaceable filter tip (3) is conical, and outer ring threads (3-1) are arranged at the bottom of the replaceable filter tip and are in threaded connection with the inner threaded hole (2-3) of the filter bracket (2); the section 1/3-3/5 away from the top end has no outer wall, the top end is connected with the vertebral body by more than two connecting ribs (3-2), and the interior is filled with the filtering matrix (3-3).
8. The metabolite extraction kit according to claim 7: the method is characterized in that: the number of the connecting ribs (3-2) is 3 or 4.
9. The metabolite extraction kit according to claim 1: the method is characterized in that: the nitrogen blow-drying box (4) is integrally cuboid in appearance, four corners of the nitrogen blow-drying box are provided with support columns (4-2), and the right side of the nitrogen blow-drying box is provided with a buffering air inlet (4-4) 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021289443.4U CN212748931U (en) | 2020-07-03 | 2020-07-03 | Metabonomics metabolite draws kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021289443.4U CN212748931U (en) | 2020-07-03 | 2020-07-03 | Metabonomics metabolite draws kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212748931U true CN212748931U (en) | 2021-03-19 |
Family
ID=75020814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021289443.4U Expired - Fee Related CN212748931U (en) | 2020-07-03 | 2020-07-03 | Metabonomics metabolite draws kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212748931U (en) |
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2020
- 2020-07-03 CN CN202021289443.4U patent/CN212748931U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
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Granted publication date: 20210319 |