CN212741352U

CN212741352U - Coccidia oocyst counting device

Info

Publication number: CN212741352U
Application number: CN202020642812.7U
Authority: CN
Inventors: 刘园; 刘丽丹; 黄仪娟; 王新秋; 曾莉; 翁亚彪; 谭志坚
Original assignee: Foshan Standard Bio Tech Co Ltd
Current assignee: Foshan Standard Bio Tech Co Ltd
Priority date: 2020-04-24
Filing date: 2020-04-24
Publication date: 2021-03-19
Anticipated expiration: 2030-04-24

Abstract

The utility model discloses a coccidian oocyst counting assembly, include: a base plate; the counting device comprises a base plate, a counting chamber I, a counting chamber II and a counting chamber II, wherein the base plate I is arranged on the left side of the base plate; and two cover glass sheets are adopted, the two cover glass sheets cover the tops of the first counting chamber and the second counting chamber respectively, and counting grids are carved at the bottoms of the cover glass sheets. The utility model discloses a set up the count scale mark in the counting chamber and can conveniently count, the cover glass is for separately and detachable design, can make things convenient for the cover glass to cover and take off through setting up the screens in the backup pad, and very firm, when doing benefit to further qualitative inspection, the liquid is examined in the transfer provides convenience, also convenient washing.

Description

Coccidia oocyst counting device

Technical Field

The utility model relates to a little biological laboratory detects the field, in particular to is used for coccidian oocyst counting assembly.

Background

Coccidiosis, a parasitic protozoal disease, poses a great hazard to breeding of poultry, livestock and the like, and companion animals such as dogs and cats. The disease is commonly generated in the global range, and a large amount of capital is spent for prevention and treatment every year. In view of the above, researchers in various countries have been working on the research of coccidia in the hope of obtaining an effective control scheme for the disease. Until now, coccidian vaccines for chicken have been successfully developed and widely applied in the chicken industry. Coccidian vaccines are live vaccines and risk of virus elimination during use. Meanwhile, coccidian vaccines are multivalent vaccines mixed by several insect species, and the number of each insect species has a strict and determined proportion. In order to ensure the immune effect of the vaccine during the use process and reduce the risk of virus dispersion as much as possible, the dosage of the vaccine must be ensured to be accurately controllable.

At present, each coccidian vaccine production enterprise can count the concentration of coccidian oocysts in the production process, so that the number of oocysts is calculated, and the preparation amount of each kind of oocysts in the vaccine is further determined, but the existing coccidian oocyst concentration counting device is basically a blood counting chamber. The blood counting chamber is a common cell counting tool, is commonly used for counting red blood cells, white blood cells and the like in medicine, is also commonly used for counting the number of microorganisms such as bacteria, fungi, yeasts and the like, and is a common biological tool. Although the blood counting plate is cheap, a large error exists when the coccidian oocyst concentration is counted. The main reason is that the device can only sample 4ml oocyst fluid samples for counting, and because the sampling amount is too small, the sampling amount can only be enlarged by sampling for at least dozens of times so as to reduce errors, but even the sampling amount is still small, so that the errors are large. In addition, the multiple oversampling counting results in increased labor intensity and greatly increased counting time for the skilled person, and is therefore inefficient. The above-mentioned deficiency results in a low efficiency of counting the coccidian oocyst concentration and a poor accuracy of the result.

Therefore, the coccidian oocyst counting device is designed, the counting method is comprehensively improved, the oocyst concentration can be accurately counted by sampling once, the operation is simple and efficient, the accuracy is higher, and the device is easy to clean and operate.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a coccidian oocyst counting assembly aims at solving among the prior art oocyst count inefficiency, difficult technical problem that washs and the accuracy is low.

A coccidial oocyst counting device comprising:

a base plate;

the counting device comprises a base plate, a counting chamber I, a counting chamber II and a counting chamber II, wherein the base plate I is arranged on the left side of the base plate;

and the number of the cover glass is two, the two cover glass covers the top of the first counting chamber and the top of the second counting chamber respectively, and counting grids are carved at the bottoms of the two cover glass.

Further preferably, be equipped with screens one in the backup pad one, be equipped with screens two in the backup pad three, be equipped with the screens three and the screens four that carry on the back mutually in the backup pad two, wherein screens three with the screens one subtend sets up, screens four with the screens two subtend sets up.

Further preferably, the counting grids are all gridlines.

Further preferably, the counting area of the first counting chamber is S1 and H1, the counting area of the second counting chamber is S2 and H2, S1< S2, H1> H2, and S1 ═ H1 ═ S2 ═ H2.

Further preferably, the supporting plate and the bottom plate are made of transparent materials.

Further preferably, the thickness of the bottom plate is 0.5-5mm, and the height of the supporting plate is 1-5 mm.

Further preferably, the thickness of the cover glass is 0.5-4 mm.

Compared with the prior art, the beneficial effects of the utility model are as follows:

(1) the utility model can be conveniently counted by arranging the counting scale lines in the counting chamber, the cover glass is in a separated and detachable design, the cover glass can be conveniently covered and taken down by arranging the clamping position on the supporting plate, and the cover glass is very firm, thereby being beneficial to further qualitative examination, providing convenience for transferring the examination liquid and being convenient for cleaning;

(2) when the utility model is used for sampling, the one-time sampling quantity is dozens of times or even hundreds of times of the one-time sampling quantity of the blood counting chamber, so that the efficiency can be greatly improved and the accuracy can be ensured when the oocysts are counted;

(3) with the same volume is designed into to the counting chamber of this utility device both sides, but two kinds of specifications of counting chamber count area and highly difference just so can select different counting chambers to carry out the concentration count according to the concentration size of sample. The counting chamber with small counting area and high height is selected when the concentration is low, so that the observation area under a microscope for counting can be reduced, further counting time is saved, and the efficiency is improved.

The application principle is as follows: because the density of the coccidian oocyst liquid is close to that of water, the floating counting method is adopted when the device is used for counting. The method is that a solution which has high density and does not affect the oocysts of the coccidia is added into the oocyst solution, so that the density of the solution is improved, and the oocysts are floated.

Drawings

FIG. 1 is a schematic structural diagram of a coccidian oocyst counting device;

FIG. 2 is a schematic sectional view of the coccidian oocyst counting device.

Detailed Description

In order to make the technical solutions of the present invention more clearly apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples do not limit the scope of the present invention.

Examples

As shown in fig. 1 and 2: a coccidial oocyst counting device comprising:

a base plate 1;

the support plate 2 comprises a first support plate 3, a second support plate 4 and a third support plate 5, the first support plate 3 and the third support plate 5 are respectively arranged on the left side and the right side of the bottom plate 1, the second support plate 4 is arranged in the middle of the bottom plate 1, a first counting chamber 6 is formed between the second support plate 4 and the first support plate 3, and a second counting chamber 7 is formed between the second support plate 4 and the third support plate 5;

the number of the cover glass 8 is two, the two cover glass 8 cover the tops of the first counting chamber 6 and the second counting chamber 7 respectively, and counting grids 13 are carved at the bottom of the cover glass 8.

Implementation materials:

high density oocyst fluid: adding NaCl saturated solution and MgSO into 1ml coccidian oocyst solution sample₄And (3) uniformly mixing 9ml of mixed solution (volume is 1:1) of the saturated solution to obtain the product.

When the utility model is used for the coccidian oocyst counting device, the two ports of the first counting chamber 6 and the second counting chamber 7 can be filled with liquid, which is convenient and fast. Specifically, the cover glass 8 is covered in the clamping position, then high-density oocyst liquid is injected into the first counting chamber 6 and the second counting chamber 7, and counting is observed after all oocysts completely float to the liquid surface.

More specifically, the uniformly mixed high-density oocyst solution is injected into the counting chamber by using a sampler, and after all oocysts completely float to the liquid surface, all oocysts in the counting chamber can be counted under a microscope. The concentration of the oocyst fluid can be obtained by calculation according to the counting result. If the volume of the counting chamber of the device is V, the counting result of all target oocysts in the counting chamber is N, and the counting device is operated, the density of the oocyst liquid is calculated according to the following formula: N/V10.

As shown in fig. 1: the first support plate 3 is provided with a first clamping position 9, the third support plate 5 is provided with a second clamping position 10, the second support plate (4) is provided with a third clamping position 11 and a fourth clamping position 12 which are opposite to each other, wherein the third clamping position 11 and the first clamping position 9 are oppositely arranged, and the fourth clamping position 12 and the second clamping position 10 are oppositely arranged. When cleaning, the cover glass 8 can be taken down and cleaned respectively, so that dirt can be cleaned. The cover glass 8 can be placed on the base plate or the base plate 1 can be placed separately from the cover glass 8. The cover glass 8 is covered on the clamping position, and the taking is convenient and firm.

For accurate counting, the bottom of the cover glass 8 is provided with counting grids 13, and the counting grids 13 are all grid lines. Satisfied this practicality and adopted the suspension method to calculate the oocyst number, once only the sample volume is equivalent to tens of times or even hundred times of the blood count board once sample volume, like this when the oocyst count, can promote efficiency greatly, but also can guarantee the accuracy.

Furthermore, the counting area of the first counting chamber 6 is S1 and H1, the counting area of the second counting chamber 7 is S2 and H2, S1< S2, H1> H2, and S1 ═ H1 ═ S2 ═ H2. The counting chambers on the two sides of the device are designed to have the same volume, but the counting chambers have different counting areas and different heights, so that different counting chambers can be selected to count the concentration according to the concentration of a sample, and further counting time is saved, so that the efficiency is improved (the counting chambers with small counting areas and high heights are selected when the concentration is low, and the observation area during counting under a microscope can be reduced, so that the time is saved).

Further, the thickness of the bottom plate 1 is 0.5-5mm, and the height of the support plate 2 is 1-5 mm.

Further, the supporting plate 2 and the bottom plate 1 are made of transparent materials. Preferably, the glass material is convenient for observation and cleaning.

The foregoing clearly describes the coccidian oocyst counting device of the present invention and the method of use thereof. The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not intended to limit the present invention in any way, and it will be apparent to those skilled in the art that the present invention can be modified and practiced with many modifications, equivalents, and alternatives falling within the scope of the present invention.

Claims

1. A coccidian oocyst counting device, comprising:

a base plate (1);

the counting device comprises a supporting plate (2), wherein the supporting plate (2) comprises a first supporting plate (3), a second supporting plate (4) and a third supporting plate (5), the first supporting plate (3) and the third supporting plate (5) are respectively arranged on the left side and the right side of a bottom plate (1), the second supporting plate (4) is arranged in the middle of the bottom plate (1), a first counting chamber (6) is formed between the second supporting plate (4) and the first supporting plate (3), and a second counting chamber (7) is formed between the second supporting plate (4) and the third supporting plate (5);

the number of the cover glass sheets (8) is two, the cover glass sheets (8) cover the tops of the counting chamber I (6) and the counting chamber II (7) respectively, and counting grids (13) are carved on the bottom surfaces of the cover glass sheets (8).

2. The coccidian oocyst counting device of claim 1, wherein a first clamping position (9) is arranged on the first support plate (3), a second clamping position (10) is arranged on the third support plate (5), and a third clamping position (11) and a fourth clamping position (12) which are opposite to each other are arranged on the second support plate (4), wherein the third clamping position (11) and the first clamping position (9) are arranged oppositely, and the fourth clamping position (12) and the second clamping position (10) are arranged oppositely.

3. The coccidian oocyst counting device of claim 1, wherein the counting cells (13) are all gridlines.

4. The coccidian oocyst counting device of claim 1, wherein the counting area of counting chamber one (6) is S1 and H1, the counting area of counting chamber two (7) is S2 and H2, the S1< S2, the H1> H2, the S1H 1H 2H 2.

5. The coccidian oocyst counting device of claim 1, wherein the support plate (2) and the bottom plate (1) are made of transparent materials.

6. The coccidian oocyst counting device according to claim 1, wherein the thickness of the bottom plate (1) is 0.5-5mm and the height of the support plate (2) is 1-5 mm.

7. The coccidian oocyst counting device of claim 1, wherein the thickness of the cover glass (8) is 0.5-4 mm.