CN212581869U - Special test piece fixing device is cultivateed to biomembrane - Google Patents

Abstract

The utility model relates to a special test piece fixing device is cultivateed to biomembrane, including the orifice plate lid, its characterized in that: the hole plate cover is characterized in that a plurality of test piece holders are arranged on the inner surface of the top of the hole plate cover and correspond to hole positions on the hole plate one by one, each test piece holder comprises a connecting seat and a test piece holding member, the upper end of the connecting seat is installed on the hole plate cover, the test piece holding member is connected to the lower end of the connecting seat, and an opening for a test piece to be installed is formed in the lower end of the test piece holding member. The utility model discloses the structure is ingenious, reasonable in design, centre gripping test piece that can be stable, makes the test piece perpendicular and the orifice plate bottom, and the effectual experimental time that shortens simplifies the operation flow is applicable to in the experiment that the biomembrane was cultivateed.

Description

Special test piece fixing device is cultivateed to biomembrane

Technical Field

The utility model relates to an accessory of orifice plate is cultivateed to biomembrane, and specifically speaking is a special test piece fixing device is cultivateed to biomembrane belongs to experimental apparatus design technical field.

Background

The human mouth contains a large number of bacteria, some of which are beneficial to our health, and some of which may cause caries or initiate an inflammatory response. Different bacteria adhere to the surface of the dental surface or restoration to form dental plaque, where they grow, develop, multiply and die, and carry out complex metabolic activities. Once plaque biofilm forms, it is not easily washed or rinsed away with water. How to control the formation of dental plaque biofilm is a research hotspot of the oral scholars.

To better simulate the process of tooth surface biofilm formation in vitro, one vertically placed a circular test piece of hydroxyapatite in a 24-well plate containing bacterial solution and culture medium, allowed bacteria to adhere to the test piece and gradually formed a biofilm over the next 43 hours. However, it is a difficult point how to stably clamp the test piece and ensure that the test piece is completely vertical to the orifice plate in the whole experimental process. The common elastic snap ring that is bent to a steel wire in the experiment at present, it can centre gripping test piece both sides to utilize the draw-in groove on the snap ring, fix the test piece in the orifice plate. But because the test piece is circular and the side is comparatively smooth, be difficult to implement stable centre gripping, be difficult to avoid leading to the test piece slippage in high temperature disinfection, liquid changing and final sampling process, influence the experimental result.

Disclosure of Invention

The utility model aims to overcome the not enough of existence among the prior art, provide a special test piece fixing device is cultivateed to biomembrane, its structure is ingenious, reasonable in design, centre gripping test piece that can be stable makes the test piece perpendicular and the orifice plate bottom, and the effectual experimental time that shortens simplifies the operation flow is applicable to in the experiment that the biomembrane was cultivateed.

According to the utility model provides a technical scheme: special test piece fixing device is cultivateed to biomembrane, including the orifice plate lid, its characterized in that: the hole plate cover is characterized in that a plurality of test piece holders are arranged on the inner surface of the top of the hole plate cover and correspond to hole positions on the hole plate one by one, each test piece holder comprises a connecting seat and a test piece holding member, the upper end of the connecting seat is installed on the hole plate cover, the test piece holding member is connected to the lower end of the connecting seat, and an opening for a test piece to be installed is formed in the lower end of the test piece holding member.

As a further improvement of the utility model, the test piece clamping member comprises a U-shaped elastic ring, an upper positioning rod, a side positioning rod and a rear stop lever, one end of the upper positioning rod is fixedly connected to the middle part of the U-shaped elastic ring and is approximately perpendicular to the plane where the U-shaped elastic ring is located, and the lower end of the connecting seat is fixedly connected with the upper side surface of the upper positioning rod; the central angle corresponding to the U-shaped elastic ring is larger than 180 degrees, the opening of the U-shaped elastic ring faces downwards, two side positioning rods which are approximately perpendicular to the plane where the U-shaped elastic ring is located are fixed on the left side and the right side of the U-shaped elastic ring, the two side positioning rods are bilaterally symmetrical and lower than the center of the U-shaped elastic ring in height, and one ends, far away from the U-shaped elastic ring, of the upper positioning rod and the two side positioning rods gradually approach to each other; the left and right sides of major arc shape elastic ring all is provided with the back pin that extends to the interior region of circle, the back pin bilateral symmetry of both sides, and the tie point between back pin and the major arc shape elastic ring lies in the district's section between last locating lever and the side locating lever.

As a further improvement of the present invention, the test piece clamping member comprises an arc-shaped clamping ring capable of elastically deforming, and the arc-shaped clamping ring is provided with an opening for loading the test piece, and the opening is arranged downwards; the middle part of the arc-shaped clamping ring is upwards connected with the lower end of the connecting seat, the arc-shaped clamping ring is in a cone cylinder shape, the inner diameter of the large head end of the arc-shaped clamping ring is smaller than the diameter of a test piece, two side separation blades perpendicular to the axial line of the arc-shaped clamping ring are arranged at the large head end of the arc-shaped clamping ring, and the two side separation blades are symmetrically distributed on two sides of the opening.

As a further improvement of the utility model, the opening part of the arc-shaped clamping ring is respectively provided with an outward bending part of the opening.

As a further improvement, the arc-shaped clamping ring is designed to be a hollow structure.

As a further improvement, be equipped with the mounting hole with test piece holder one-to-one on the top internal surface of orifice plate lid, connecting seat upper end interference fit or threaded connection are in the mounting hole.

As a further improvement of the utility model, be provided with the gasket that can dismantle the change between the bottom of mounting hole and the connecting seat upper end surface.

As a further improvement of the utility model, the connecting seat comprises a V-shaped spreading clamp, the upper end of the V-shaped spreading clamp is provided with a ring buckle, and the middle part of the V-shaped spreading clamp is provided with an arc-shaped bent bulge; the orifice plate cover is provided with a mounting through hole, the inner surface of the lower part of the mounting through hole is provided with a concave arc-shaped cavity, and the concave arc-shaped cavity is matched with the arc-shaped bent convex shape; the test piece clamping member comprises two arc clamping arms which are symmetrically arranged and can elastically deform, the upper ends of the two arc clamping arms are respectively connected with the lower ends of the two support arms of the V-shaped spreading clamp, an opening for the test piece to be loaded is reserved between the lower ends of the two arc clamping arms, and clamping grooves for positioning the test piece are formed in the inner side surfaces of the two arc clamping arms.

As a further improvement of the utility model, the arc-shaped clamping arm is designed to be a hollow structure.

As a further improvement of the utility model, the test piece holder is made of metal or plastic.

Compared with the prior art, the utility model, have following advantage:

1) the utility model has the advantages of ingenious structure and reasonable design, the test piece holder on the pore plate cover can easily and stably hold and fix the test piece, the test piece can be vertically fixed in the pore plate only by covering the pore plate cover on the pore plate after the test piece is clamped, each test piece is ensured to be vertical to the bottom surface by 90 degrees, and the test piece can be installed in place easily; and the top centre gripping mode is more firm, can avoid the test piece to drop in the experimentation. When the liquid is changed, the hole plate cover is replaced, so that the cleaning and liquid changing work of a plurality of test pieces can be completed at one time, and the operation time is greatly shortened. The sample is scribbled the board and also only need pick up the orifice plate lid and can take out the test piece is whole from the orifice plate, need not direct contact, has overcome the problem of the easy microbiological contamination when taking off the test piece among the prior art.

2) The utility model discloses a three kinds of test piece holders all can easily strut, make the test piece can pack into fast. After the test piece is loaded, the plurality of parts in the test piece holder are matched with each other, so that the test piece can be grasped, the test piece is prevented from slipping from the side surface, and the test piece can be reliably and stably fixed after being loaded. And the area of contact between test piece clamping member and the test piece is little, makes the test piece surface have bigger area to form the biomembrane, makes things convenient for the sample in later stage.

Drawings

Fig. 1 is a cross-sectional view of the structure and application of embodiment 1 of the present invention.

Fig. 2 is an enlarged schematic view of a portion a in fig. 1.

Fig. 3 is a front view of a test piece holder according to embodiment 1 of the present invention.

Fig. 4 is a structural side view of a test piece holder in embodiment 1 of the present invention.

Fig. 5 is a front view of a test piece holder according to embodiment 2 of the present invention.

Fig. 6 is a sectional view taken along line B-B in fig. 5.

Fig. 7 is a cross-sectional view of the structure and application of embodiment 3 of the present invention.

Fig. 8 is an enlarged schematic view of the portion C in fig. 7.

Description of reference numerals: the test device comprises a 1-hole plate cover, a 2-test piece holder, a 3-hole plate, an 11-mounting hole, a 12-mounting through hole, a 13-concave arc cavity, a 21-connecting seat, a 22-test piece holding member, a 23-opening, a 211-V-shaped spreading clamp, a 212-buckle, a 213-arc bending convex part, a 221-major arc elastic ring, a 222-upper positioning rod, a 223-side positioning rod, a 224-rear blocking rod, a 225-arc clamping ring, a 226-side blocking piece, a 227-outer bending part, a 228-arc clamping arm and a 229-embedded groove.

Detailed Description

The invention is further described with reference to the following specific drawings and examples.

Example 1

As shown in fig. 1 to 4, embodiment 1 discloses a special test piece fixing device for biofilm culture, which includes a hole plate cover 1, wherein a plurality of test piece holders 2 are arranged on an inner surface of a top of the hole plate cover 1, the plurality of test piece holders 2 are in one-to-one correspondence with hole sites on a hole plate 3, each test piece holder 2 includes a connecting seat 21 and a test piece holding member 22, an upper end of the connecting seat 21 is mounted on the hole plate cover 1, the test piece holding member 22 is connected to a lower end of the connecting seat 21, and an opening 23 for a test piece to be mounted is arranged at a lower end of the test piece holding member 22.

When the test piece clamping device is used specifically, a test piece is firstly loaded into the test piece clamping member 22 from the lower end opening 23 of the test piece clamping member 22, and the test piece can be easily and stably clamped and fixed by the test piece clamping member 22. After all the test piece holders 2 are clamped with the test pieces, the hole plate cover 1 is covered on a hole plate 3 (a 24-hole plate in the figure), so that the test pieces can be vertically fixed in the hole plate 3, each test piece is ensured to be vertical to the bottom surface at 90 degrees, and the test pieces can be installed in place easily; and because the test piece is top centre gripping mode, it is more firm than current side centre gripping mode, can avoid the test piece to drop in the experimentation. When liquid is changed, the hole plate cover 1 can be directly changed, so that the cleaning and liquid changing work of 24 test pieces can be completed at one time, and the operation time is greatly shortened. After the completion is cultivateed to the biomembrane, if need the sample scribble the board, only need take up orifice plate lid 1 can take out the test piece is whole from orifice plate 3, take off very conveniently, need not direct contact, the problem of the easy microbiological contamination when having overcome the test piece of taking off in the prior art.

As shown in fig. 3 and 4, in the present embodiment 1, the specimen clamping member 22 includes a u-arc elastic ring 221, an upper positioning rod 222, a side positioning rod 223 and a rear stop rod 224, one end of the upper positioning rod 222 is fixedly connected to the middle of the u-arc elastic ring 221 and approximately perpendicular to the plane of the u-arc elastic ring 221, and the lower end of the connecting seat 21 is fixedly connected to the upper side surface of the upper positioning rod 222; the central angle corresponding to the excellent arc-shaped elastic ring 221 is greater than 180 degrees, the opening 23 of the excellent arc-shaped elastic ring 221 faces downwards, two side positioning rods 223 approximately perpendicular to the plane where the excellent arc-shaped elastic ring 221 is located are fixed on the left side and the right side of the excellent arc-shaped elastic ring 221, the two side positioning rods 223 are bilaterally symmetrical and are lower than the center of the excellent arc-shaped elastic ring 221 in height, and one ends of the upper positioning rod 222 and the two side positioning rods 223, which are far away from the excellent arc-shaped elastic ring 221, are gradually closed; the left and right sides of the major arc-shaped elastic ring 221 are both provided with rear stop levers 224 extending to the inner area of the ring, the rear stop levers 224 on the two sides are bilaterally symmetrical, and the connection point between the rear stop lever 224 and the major arc-shaped elastic ring 221 is located in the section between the upper positioning rod 222 and the side positioning rod 223. With such an arrangement, the major arc elastic ring 221 can be easily expanded, so that the test piece can be quickly loaded. After the test piece is packed into, go up locating lever 222, side locating lever 223 and back pin 224 and fix the spacing to the test piece from four directions, simultaneously because the one end of keeping away from excellent arc elastic ring 221 of last locating lever 222 and two side locating levers 223 is the trend of drawing close gradually, can "grasp" the test piece like this, avoid the test piece slippage from the side for the test piece obtains reliable and reliable fixed after packing into. Moreover, the contact area between the test piece clamping member 22 with the structure and the test piece is small, so that the surface of the test piece can have a larger area to form a biological film, and the later-stage sampling is convenient.

As shown in fig. 1 and 2, in this embodiment 2, mounting holes 11 corresponding to the specimen holders 2 one to one are provided on the inner surface of the top of the aperture plate cover 1, and the upper ends of the connecting bases 21 are in interference fit with the mounting holes 11. So set up, be removable assecmbly's form between test piece holder 2 and orifice plate lid 1, when one of them or certain several test piece holder 2 damages and need change or need not use, can dismantle fast. In practical applications, the upper end of the connecting seat 21 and the mounting hole 11 may be in a threaded connection. In practical production applications, it is preferable that a gasket for detachable replacement is arranged between the bottom of the mounting hole 11 and the upper end surface of the connecting seat 21, so that the height of the test piece holder 2 can be properly adjusted to meet experimental requirements.

The utility model discloses in, test piece holder 2 can adopt metal or plastics to make, and test piece clamping member 22 as in embodiment 1 preferably adopts the steel wire bending system welding to form.

Example 2

As shown in fig. 5 and 6, example 2 discloses a specimen holder for biofilm culture, which is different from example 1 in the structure of a specimen holding member 22.

In this embodiment 2, the specimen-clamping member 22 includes an elastically deformable arc-shaped clamping ring 225, the arc-shaped clamping ring 225 is provided with an opening 23 for the specimen to be loaded, and the opening 23 is disposed downward; the middle part of the arc-shaped clamping ring 225 is upwards connected with the lower end of the connecting seat 21, the arc-shaped clamping ring 225 is in a cone cylinder shape, the inner diameter of the large end of the arc-shaped clamping ring 225 is smaller than the diameter of a test piece, two side blocking pieces 226 perpendicular to the axial lead of the arc-shaped clamping ring 225 are arranged at the large end of the arc-shaped clamping ring 225, and the two side blocking pieces 226 are symmetrically distributed on two sides of the opening 23. So set up, when the test piece was packed into in the arc grip ring 225, the arc grip ring 225 can hold the test piece firmly, and side separation blade 226 can avoid the test piece slippage from the side.

As shown in fig. 5 and 6, in the present embodiment 2, the openings 23 of the arc-shaped clamping rings 225 are respectively provided with an outer bent portion 227 facing away from the openings 23. By the arrangement, a round test piece can be more conveniently loaded into the clamp. When the test piece needs to be taken out, the opening 23 of the arc-shaped clamping ring 225 can be opened by pushing the outer bending part 227, so that the test piece can fall freely, and the situation that the biological film on the surface of the test piece is damaged due to the fact that the test piece is clamped and pulled is avoided.

In this embodiment 2, the arc-shaped clamping ring 225 is preferably designed to be a hollow structure (not shown in the figure). So set up, can reduce the area of contact between arc grip ring 225 and the test piece, make the test piece surface can have bigger area to form the biomembrane, make things convenient for the sample in later stage.

Example 3

As shown in fig. 7 and 8, example 3 discloses a test piece fixing device for biofilm culture, which is different from example 1 in that: the specimen holder 2 in embodiment 3 can automatically open to release the specimen.

As shown in fig. 7 and 8, in this embodiment 3, the connecting seat 21 includes a V-shaped spreading clamp 211, a buckle 212 is disposed at an upper end of the V-shaped spreading clamp 211, and an arc-shaped protrusion 213 is disposed at a middle portion of the V-shaped spreading clamp 211; the perforated plate cover 1 is provided with a mounting through hole 12, the inner surface of the lower part of the mounting through hole 12 is provided with a concave arc-shaped cavity 13, and the concave arc-shaped cavity 13 is matched with the arc-shaped bent bulge 213 in shape; the test piece clamping member 22 comprises two symmetrically arranged and elastically deformable arc-shaped clamping arms 228, the upper ends of the two arc-shaped clamping arms 228 are respectively and integrally connected with the lower ends of the two support arms of the V-shaped spreading clamp 211, an opening 23 for a test piece to be loaded is reserved between the lower ends of the two arc-shaped clamping arms 228, and the inner side surfaces of the two arc-shaped clamping arms 228 are provided with clamping grooves 229 for positioning the test piece. With the arrangement, when the arc-shaped bent boss 213 on the connecting seat 21 is positioned in the concave arc-shaped cavity 13, a test piece is arranged between the two arc-shaped clamping arms 228 and can be firmly clamped, and meanwhile, the clamping groove 229 can position the test piece, so that the test piece is prevented from deflecting and moving; when the test piece needs to be taken out, the connecting seat 21 is slightly pushed downwards with force, when the arc-shaped bent boss 213 leaves the concave arc-shaped cavity 13, the two support arms of the V-shaped spreading clamp 211 automatically spring open and drive the two arc-shaped clamping arms 228 to separate, and the test piece is released and automatically falls from the opening 23. After the test piece is released, the fingers are inserted into the ring buckle 212, the V-shaped expanding clamp 211 is pulled upwards with force, the arc-shaped bent boss 213 is clamped into the concave arc-shaped cavity 13 again, and the next time the test piece is loaded is waited.

In this embodiment 3, the arc-shaped holding arm 228 is also preferably configured to be a hollow structure.

The above description is only for the preferred embodiment of the present invention, and the above specific embodiments are not intended to limit the present invention. Within the scope of the technical idea of the present invention, various modifications and alterations can be made, and any person skilled in the art can make modifications, amendments or equivalent replacements according to the above description, all belonging to the protection scope of the present invention.

Claims (10)

1. Special test piece fixing device is cultivateed to biomembrane, including orifice plate lid (1), its characterized in that: the hole plate clamping device is characterized in that a plurality of test piece clamping devices (2) are arranged on the inner surface of the top of the hole plate cover (1), the test piece clamping devices (2) correspond to hole positions on the hole plate (3) in a one-to-one mode, each test piece clamping device (2) comprises a connecting seat (21) and a test piece clamping component (22), the upper end of each connecting seat (21) is installed on the hole plate cover (1), the test piece clamping components (22) are connected to the lower ends of the connecting seats (21), and openings (23) for test pieces to be installed are formed in the lower ends of the test piece clamping components.

2. The special test piece fixing device for biofilm culture according to claim 1, characterized in that: the test piece clamping member (22) comprises a major arc-shaped elastic ring (221), an upper positioning rod (222), a side positioning rod (223) and a rear stop rod (224), one end of the upper positioning rod (222) is fixedly connected to the middle of the major arc-shaped elastic ring (221) and approximately perpendicular to the plane where the major arc-shaped elastic ring (221) is located, and the lower end of the connecting seat (21) is fixedly connected with the upper side face of the upper positioning rod (222); the central angle corresponding to the excellent arc-shaped elastic ring (221) is larger than 180 degrees, the opening (23) of the excellent arc-shaped elastic ring (221) faces downwards, the left side and the right side of the excellent arc-shaped elastic ring (221) are respectively and fixedly provided with a side positioning rod (223) which is approximately vertical to the plane where the excellent arc-shaped elastic ring (221) is located, the two side positioning rods (223) are bilaterally symmetrical and are lower than the center of the excellent arc-shaped elastic ring (221), and one ends of the upper positioning rod (222) and the two side positioning rods (223) which are far away from the excellent arc-shaped elastic ring (221) gradually approach to each other; the left and right sides of major arc shape elastic ring (221) all are provided with back pin (224) to the regional extension in the circle, back pin (224) bilateral symmetry of both sides, and the tie point between back pin (224) and major arc shape elastic ring (221) is located the district's section between upper boom (222) and side boom (223).

3. The special test piece fixing device for biofilm culture according to claim 1, characterized in that: the test piece clamping member (22) comprises an arc-shaped clamping ring (225) capable of elastically deforming, an opening (23) for a test piece to be installed is formed in the arc-shaped clamping ring (225), and the opening (23) is arranged downwards; arc clamping ring (225) middle part upwards is connected with connecting seat (21) lower extreme, and arc clamping ring (225) are the cone cylindric, and the internal diameter of arc clamping ring (225) stub end is less than the diameter of test piece, and the stub end of arc clamping ring (225) is equipped with side separation blade (226) of two perpendicular to arc clamping ring (225) axial lead, and two side separation blade (226) symmetric distribution are in opening (23) both sides.

4. The special test piece fixing device for biofilm culture according to claim 3, characterized in that: the openings (23) of the arc-shaped clamping rings (225) are respectively provided with an outer bending part (227) which faces away from the openings (23).

5. The special test piece fixing device for biofilm culture according to claim 3, characterized in that: the arc-shaped clamping ring (225) is of a hollow structure.

6. The special test piece fixing device for biofilm culture according to claim 1, characterized in that: the inner surface of the top of the orifice plate cover (1) is provided with mounting holes (11) which correspond to the test piece holders (2) one by one, and the upper end of the connecting seat (21) is in interference fit or threaded connection in the mounting holes (11).

7. The special test piece fixing device for biofilm culture according to claim 6, characterized in that: a detachable and replaceable gasket is arranged between the bottom of the mounting hole (11) and the upper end surface of the connecting seat (21).

8. The special test piece fixing device for biofilm culture according to claim 1, characterized in that: the connecting seat (21) comprises a V-shaped spreading clamp (211), the upper end of the V-shaped spreading clamp (211) is provided with a buckle (212), and the middle part of the V-shaped spreading clamp (211) is provided with an arc-shaped bent bulge (213); the perforated plate cover (1) is provided with a mounting through hole (12), the inner surface of the lower part of the mounting through hole (12) is provided with a concave arc-shaped cavity (13), and the concave arc-shaped cavity (13) is matched with the arc-shaped bent bulge (213) in shape; the test piece clamping member (22) comprises two arc-shaped clamping arms (228) which are symmetrically arranged and can elastically deform, the upper ends of the two arc-shaped clamping arms (228) are integrally connected with the lower ends of the two support arms of the V-shaped spreading clamp (211) respectively, an opening (23) for a test piece to be loaded into is reserved between the lower ends of the two arc-shaped clamping arms (228), and an embedded clamping groove (229) for positioning the test piece is formed in the inner side surfaces of the two arc-shaped clamping arms (228).

9. The special test piece fixing device for biofilm culture according to claim 8, wherein: the arc-shaped clamping arm (228) is of a hollow structure.

10. The special test piece fixing device for biofilm culture according to any one of claims 1 to 9, wherein: the test piece holder (2) is made of metal or plastic.

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