CN212545283U

CN212545283U - Living cell cryopreservation device

Info

Publication number: CN212545283U
Application number: CN202020857801.0U
Authority: CN
Inventors: 夏萍
Original assignee: Shenzhen Jiexika Biotechnology Co ltd
Current assignee: Shenzhen Jiexika Biotechnology Co ltd
Priority date: 2020-05-21
Filing date: 2020-05-21
Publication date: 2021-02-19
Anticipated expiration: 2030-05-21

Abstract

The utility model relates to the technical field of cell cryopreservation, in particular to a living cell cryopreservation device, which comprises a box body, wherein a supporting block is welded on the inner wall of the box body, a storage box is welded on the inner side of the supporting block, sliding grooves are respectively formed in the left side and the right side of the inner cavity of the storage box, the inner cavity of each sliding groove is slidably connected with a concave type supporting frame, a clamping block is inserted into the inner cavity of each concave type supporting frame, and a storage plate is welded on the inner side of each clamping block; the utility model discloses a box, supporting shoe, case, spout, concave type support frame, fixture block, the setting of depositing board, circular slot, spring, splint, feed liquor pipe and fluid-discharge tube, solved the live body cell and placed generally and deposit in the cell ware, then freeze the save to the cell ware to freeze the save to the live body cell, present live body cell freeze save set, the volume is too big, is not convenient for remove, operates complicated problem when fixing to the live body cell ware.

Description

Living cell cryopreservation device

Technical Field

The utility model relates to a cell freezes deposits technical field, specifically is a living cell freezes save set.

Background

The cell freezing is one of the main methods for cell preservation, the freezing technology is utilized to place the cells in liquid nitrogen at the temperature of-196 ℃ for low-temperature preservation, the cells can be temporarily separated from the growth state and the cell characteristics can be preserved, so that the cells are revived for experiments when needed, a certain amount of cells are preserved moderately, the cells can be prevented from being lost due to the pollution or other accidents of the cells being cultured, and the effect of cell preservation is achieved.

In order to solve the above problems, a conventional living cell cryopreservation apparatus is too large in size, inconvenient to move, and complicated to fix the living cell dish.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a living cell cryopreservation device, it is small to possess, and it is convenient to remove, and easy operation's advantage when fixed to living cell dish has solved living cell and has placed generally and deposit in the cell dish, then freezes to preserve the cell dish to freezing the preservation to living cell, present living cell cryopreservation device, the volume is too big, is not convenient for remove, operates complicated problem when fixing living cell dish.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a living cell cryopreservation device, includes the box, the inner wall welding of box has the supporting shoe, the inboard welding of supporting shoe has the case, the spout has all been seted up to the left and right sides of case inner chamber, the inner chamber sliding connection of spout has concave type support frame, the inner chamber of concave type support frame is pegged graft and is had the fixture block, the inboard welding of fixture block has the board of depositing, the circular slot has been seted up to the top equidistance of the board of depositing, the impartial distance welding in the left and right sides of circular slot inner chamber has the spring, the one side welding that the circular slot was kept away from to the spring has splint, the left side intercommunication at box top has the feed liquor.

Preferably, the connecting plates are welded on two sides of the bottom of the storage plate, the supporting plate is welded on the inner side of the connecting plates, and the sponge cushion is bonded on the top of the supporting plate.

Preferably, the front surface of the concave support frame is in threaded connection with a fixing bolt, and the back surface of the fixing bolt penetrates through the concave support frame and extends to the back surface of the inner cavity of the sliding groove.

Preferably, the handle is welded at the center of the top of the box body, and the pull rod is bolted to the back of the box body.

Preferably, a first gate valve is fixedly mounted at the top of the surface of the liquid discharge pipe, and a second gate valve is fixedly mounted on the left side of the surface of the liquid inlet pipe.

Preferably, the positive left side of case articulates there is the dodge gate, the positive right side welding of dodge gate has the drawing piece, the positive right side of dodge gate just is located the right side of drawing the piece and inlays and be equipped with the lock.

Preferably, the four corners department of bottom half all welds and has the supporting leg, the bottom fixed mounting of supporting leg has the universal wheel.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses a box, supporting shoe, case, spout, concave type support frame, fixture block, the setting of depositing board, circular slot, spring, splint, feed liquor pipe and fluid-discharge tube, solved the live body cell and placed generally and deposit in the cell ware, then freeze the save to the cell ware to freeze the save to the live body cell, present live body cell freeze save set, the volume is too big, is not convenient for remove, operates complicated problem when fixing to the live body cell ware.

2. The utility model discloses a setting of connecting plate, connecting plate can support the backup pad, and the backup pad can support foam-rubber cushion and cell ware, and the foam-rubber cushion can protect the cell ware, and through fixing bolt's setting, fixing bolt can fix concave type support frame, and the spout is convenient for concave type support frame and slides, through the setting of handle, the removal of the box of being convenient for of handle, the pull rod is convenient to the pulling of box for the removal of box is more laborsaving.

3. The utility model discloses a setting of first gate valve, first gate valve can be opened or close the fluid-discharge tube, opening or closing of the steerable feed liquor pipe of second gate valve, can be to pouring into the liquid nitrogen between box and the case through the feed liquor pipe, setting through the dodge gate, the dodge gate can be protected the inside of case, the pull block is convenient for to opening and closing the dodge gate, the lock can be fixed the dodge gate, through the setting of supporting leg, the supporting leg can support the box, the universal wheel is convenient for the removal of supporting leg, thereby make the box remove.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a front view of the cross-sectional structure of the present invention;

FIG. 3 is a rear view of the structure of the present invention;

FIG. 4 is a top view of the cross-sectional structure of the present invention;

fig. 5 is an enlarged schematic view of the area a in the second drawing of the present invention.

In the figure: 1. a box body; 2. a movable door; 3. a liquid discharge pipe; 4. a handle; 5. a liquid inlet pipe; 6. supporting legs; 7. a storage box; 8. a concave support frame; 9. a chute; 10. a clamping block; 11. a sponge cushion; 12. a support block; 13. a support plate; 14. a connecting plate; 15. a circular groove; 16. a storage plate; 17. fixing the bolt; 18. A splint; 19. a spring; 20. a pull rod.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-5, a living cell cryopreservation device comprises a box body 1, wherein a supporting block 12 is welded on the inner wall of the box body 1, a storage box 7 is welded on the inner side of the supporting block 12, sliding grooves 9 are formed in the left side and the right side of the inner cavity of the storage box 7, a concave support frame 8 is connected to the inner cavity of each sliding groove 9 in a sliding mode, a clamping block 10 is inserted into the inner cavity of each concave support frame 8, a storage plate 16 is welded on the inner side of each clamping block 10, circular grooves 15 are formed in the top of each storage plate 16 at equal intervals, springs 19 are welded on the left side and the right side of the inner cavity of each circular groove 15 at equal intervals, a clamping plate 18 is welded on one side, away from the circular grooves 15, of each spring 19, a liquid inlet pipe 5 is communicated with the left side of the top of the box body 1, a liquid outlet pipe 3, The arrangement of the storage box 7, the sliding groove 9, the concave support frame 8, the clamping block 10, the storage plate 16, the circular groove 15, the spring 19, the clamping plate 18, the liquid inlet pipe 5 and the liquid outlet pipe 3 solves the problems that the living cells are usually placed in a cell dish for storage, and then the cell dish is subjected to cryopreservation, so that the living cells are subjected to cryopreservation, and the existing living cell cryopreservation device has overlarge volume, is inconvenient to move and is complicated to operate when the living cell dish is fixed.

In this embodiment, it is concrete, the both sides of depositing 16 bottoms of board have all been welded connecting plate 14, and the inboard welding of connecting plate 14 has backup pad 13, and the top of backup pad 13 bonds there is foam-rubber cushion 11, and through the setting of connecting plate 14, connecting plate 14 can support backup pad 13, and backup pad 13 can support foam-rubber cushion 11 and cell ware, and foam-rubber cushion 11 can protect the cell ware.

In this embodiment, specifically, the front surface of the concave support frame 8 is connected with the fixing bolt 17 through threads, the back surface of the fixing bolt 17 penetrates through the concave support frame 8 and extends to the back surface of the inner cavity of the sliding groove 9, through the setting of the fixing bolt 17, the fixing bolt 17 can fix the concave support frame 8, and the sliding groove 9 facilitates the sliding of the concave support frame 8.

In this embodiment, specifically, the handle 4 is welded at the center of the top of the box body 1, the pull rod 20 is bolted to the back of the box body 1, the handle 4 is convenient for the box body 1 to move through the arrangement of the handle 4, and the pull rod 20 is convenient for pulling the box body 1, so that the box body 1 can be moved more easily.

In this embodiment, it is specific, the top fixed mounting on fluid-discharge tube 3 surface has first gate valve, and the left side fixed mounting on fluid-discharge tube 5 surface has the second gate valve, and through the setting of first gate valve, first gate valve can be opened or closed fluid-discharge tube 3, and opening or closing of the steerable fluid-discharge tube 5 of second gate valve can pour into the liquid nitrogen into between box 1 and case 7 through fluid-discharge tube 5.

In this embodiment, it is concrete, the positive left side of case 7 articulates there is dodge gate 2, and the positive right side welding of dodge gate 2 has the drawing piece, and the positive right side of dodge gate 2 just is located the right side of drawing the piece and inlays and be equipped with the lock, and through the setting of dodge gate 2, dodge gate 2 can protect the inside of case 7, draws the piece to be convenient for to opening and closing of dodge gate 2, and the lock can be fixed dodge gate 2.

In this embodiment, it is concrete, and the four corners department of box 1 bottom all welds supporting leg 6, and the bottom fixed mounting of supporting leg 6 has the universal wheel, and through the setting of supporting leg 6, supporting leg 6 can support box 1, and the universal wheel is convenient for supporting leg 6's removal to make box 1 remove.

The working principle is as follows: screwing the second gate valve to open the liquid inlet pipe 5, injecting liquid nitrogen between the box body 1 and the storage box 7 through the liquid inlet pipe 5, screwing the second gate valve to close the liquid inlet pipe 5, pulling the pull block to drive the movable door 2 to move, thereby opening the movable door 2, pulling the storage plate 16, moving the storage plate 16 to drive the fixture block 10 to move, thereby separating the fixture block 10 from the inner cavity of the concave support frame 8, then placing the living cell dish into the inner cavity of the circular groove 15, so that the outer side of the living cell dish is attached to the inner side of the fixture block 18, driving the fixture block 18 to fix the living cell dish by the spring 19, attaching the bottom of the living cell dish to the top of the sponge pad 11, protecting the living cell dish by the sponge pad 11, then moving the storage plate 16, driving the fixture block 10 to move by the storage plate 16, so that the fixture block 10 is inserted into the inner cavity of the concave support frame 8, and then screwing, make fixing bolt 17 break away from spout 9 to relieve the fixed to concave type support frame 8, remove concave type support frame 8 as required, when the position is suitable, twist again fixing bolt 17, make fixing bolt 17 fix concave type support frame 8, thereby fix depositing board 16, then close dodge gate 2, thereby carry out the cryopreservation to the live body cell in the live body cell dish.

The standard parts used in the present application document can be purchased from the market, and can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts the conventional means of mature bolt, rivet, welding and the like in the prior art, the machinery, parts and equipment adopt the conventional models in the prior art, and the structure and principle of the parts known by the skilled person can be known by the technical manual or by the conventional experimental method.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a living cell cryopreservation device, includes box (1), its characterized in that: the utility model discloses a storage box, including box (1), inner wall welding of box (1) has supporting shoe (12), the inboard welding of supporting shoe (12) has case (7), spout (9) have all been seted up to the left and right sides of case (7) inner chamber, the inner chamber sliding connection of spout (9) has concave type support frame (8), the inner chamber of concave type support frame (8) is pegged graft and is had fixture block (10), the inboard welding of fixture block (10) has storage plate (16), circular slot (15) have been seted up to the top equidistance of storage plate (16), the impartial distance welding in the left and right sides of circular slot (15) inner chamber has spring (19), one side welding that circular slot (15) were kept away from in spring (19) has splint (18), the left side intercommunication at box (1) top has feed liquor pipe (5), the bottom intercommunication on box (1).

2. A living cell cryopreservation apparatus as claimed in claim 1 wherein: the storage plate is characterized in that connecting plates (14) are welded on two sides of the bottom of the storage plate (16), a supporting plate (13) is welded on the inner side of each connecting plate (14), and a sponge pad (11) is bonded on the top of each supporting plate (13).

3. A living cell cryopreservation apparatus as claimed in claim 1 wherein: the front of concave type support frame (8) threaded connection has fixing bolt (17), the back of fixing bolt (17) runs through concave type support frame (8) and extends to the back of spout (9) inner chamber.

4. A living cell cryopreservation apparatus as claimed in claim 1 wherein: the handle (4) is welded at the center of the top of the box body (1), and the pull rod (20) is bolted to the back of the box body (1).

5. A living cell cryopreservation apparatus as claimed in claim 1 wherein: the top on the surface of the liquid discharge pipe (3) is fixedly provided with a first gate valve, and the left side on the surface of the liquid inlet pipe (5) is fixedly provided with a second gate valve.

6. A living cell cryopreservation apparatus as claimed in claim 1 wherein: the positive left side of case (7) articulates there is dodge gate (2), the positive right side welding of dodge gate (2) has the piece of drawing, the positive right side of dodge gate (2) just is located the right side of drawing the piece and inlays and be equipped with the lock.

7. A living cell cryopreservation apparatus as claimed in claim 1 wherein: supporting legs (6) are welded at four corners of the bottom of the box body (1), and universal wheels are fixedly mounted at the bottoms of the supporting legs (6).