CN212542353U - Laser mass spectrometer and laser optical component - Google Patents

Abstract

The utility model relates to a laser mass spectrometer and laser optical component, laser optical component include laser instrument, first speculum, first motor, second mirror and second motor. When the laser mass spectrometer is used for detection, the first motor drives the first reflecting mirror to swing back and forth around the first direction, the second motor drives the second reflecting mirror to swing back and forth around the second direction, so that light spots of the laser can be sequentially irradiated in a scanning mode and distributed in one detection area of a sample to be detected, then the sample stage is moved by the moving assembly of the laser mass spectrometer, and the other detection area of the sample to be detected is sequentially irradiated with the light spots of the laser in a scanning mode. Therefore, the moving assembly of the laser mass spectrometer does not need to drive the sample stage at a high frequency, and the first motor and the second motor respectively drive the first reflector and the second reflector to act, so that the working requirement of the laser at the high frequency can be met, and the mass spectrometer detection and analysis efficiency can be improved.

Description

Laser mass spectrometer and laser optical component

Technical Field

The utility model relates to a mass spectrometry detects technical field, especially relates to a laser mass spectrometry detector and laser optical component.

Background

Laser mass spectrometry is a method of mass spectrometry that relies on laser light to ionize a sample to be analyzed. In recent years, the method is widely applied to the field of analysis of inorganic trace elements, organic substances and biomolecules. The laser mass spectrometer detector has a wide variety of types, including a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, a laser sputtering ionization time-of-flight mass spectrometer, a single-particle aerosol time-of-flight mass spectrometer, a laser ionization and inductively coupled plasma mass spectrometer and the like, and particularly, the matrix-assisted laser desorption ionization time-of-flight mass spectrometer occupies a significant position in the field of life science.

However, with the development of laser technology, the laser emission frequency of the laser mass spectrometer is higher and higher, from the first dozens of Hz to thousands of Hz or even tens of thousands of Hz at present. Wherein, laser ionization mass spectrometry's rate and laser frequency are positive correlation, when the laser emission frequency of laser instrument of laser mass spectrometry detector was more and more high, the detection rate and the life of laser mass spectrometry detector can be improved to a certain extent, however laser mass spectrometry detector is carrying out the in-process that detects, sample target moving platform's removal frequency need be synchronous to be improved simultaneously, can increase the quality requirement of the driving motor and lead screw and the guide rail that are used for driving sample target moving platform like this, the drive moving platform of long-time high frequency, loss moving platform precision and stability, can't be with the synchronous stable work of high frequency laser. Thus, the detection and analysis efficiency of the laser mass spectrometer cannot be improved.

SUMMERY OF THE UTILITY MODEL

Accordingly, there is a need to overcome the drawbacks of the prior art and to provide a laser mass spectrometer and a laser optical assembly, which can improve the efficiency of mass spectrometry.

The technical scheme is as follows: a laser optic assembly, comprising: a laser for emitting laser light; the laser device comprises a first reflector and a first motor, wherein the first reflector is used for reflecting laser emitted by the laser device, the first reflector is connected with a power rotating shaft of the first motor, the setting direction of the power rotating shaft of the first motor is a first direction, and the first motor is used for driving the first reflector to swing around the first direction; the second mirror and second motor, the second mirror be used for with laser after the first mirror reflection reflects, the laser that the second mirror reflected is used for sending into the ion source cavity, the second mirror with the power pivot of second motor links to each other, the power pivot of second motor sets up the direction and is the second direction, the second motor is used for the drive the second mirror winds the second direction swings, first direction with the second direction is two not equidirectionals.

When the laser optical component performs detection, the first motor drives the first reflecting mirror to swing back and forth around the first direction, and the second motor drives the second reflecting mirror to swing back and forth around the second direction, so that light spots of the laser can be sequentially irradiated and distributed in one detection area of a sample to be detected in a scanning mode, then the sample stage can be moved by the moving component of the laser mass spectrometer, and similarly, the light spots of the laser are sequentially irradiated and distributed in the other detection area of the sample to be detected in a scanning mode. Therefore, the moving assembly of the laser mass spectrometer does not need to drive the sample table at a high frequency, and the first motor and the second motor respectively drive the first reflecting mirror and the second reflecting mirror to act, so that the working requirement of the laser at the high frequency can be met, and the mass spectrometer detection and analysis efficiency can be improved.

In one embodiment, the laser optical assembly further comprises a mounting plate, a first bracket and a second bracket; the mounting plate is used for being arranged outside the ion source cavity; the first support and the second support are arranged on the mounting plate, the first motor is arranged on the first support, and the second motor is arranged on the second support.

In one embodiment, a plurality of cooling fins are arranged on the mounting board at intervals at the position corresponding to the laser and on the side away from the laser; the mounting plate is a heat dissipation metal plate.

In one embodiment, the power rotating shaft of the first motor is perpendicular to the plate surface of the mounting plate, and the first reflector swings with the axial direction of the power rotating shaft of the first motor as a central axis; the power rotating shaft of the second motor is parallel to the plate surface of the mounting plate, and the second reflector swings with the axial direction of the power rotating shaft of the second motor as a central shaft.

In one embodiment, a first included angle is formed between the first reflecting mirror and laser emitted by the laser, and the first included angle is 40-50 degrees; a second included angle is formed between the laser reflected by the first reflector and the second reflector and is 40-50 degrees; the range of the swing angle of the first reflector is-3 degrees to 3 degrees, and the range of the swing angle of the second reflector is-3 degrees to 3 degrees.

In one embodiment, the laser optical assembly further comprises a housing, a first focusing lens, a collimating lens and a second focusing lens, wherein laser emitted by the laser generates a divergent laser beam through the first focusing lens, the divergent laser beam becomes a collimated laser beam through the collimating lens, and the collimated laser beam can be reflected by the optical reflection mechanism and then incident on the sample after being focused by the second focusing lens; the first focusing lens, the collimating lens and the second focusing lens are arranged in the shell, and the distance between the first focusing lens and the collimating lens is adjustable.

In one embodiment, the laser is a solid state laser or an N2 laser.

A laser mass spectrometer comprises the laser optical assembly, an illumination assembly, an imaging assembly and an ion source assembly; the ion source assembly comprises an ion source cavity, an optical reflection mechanism, an extraction pole piece, a sample stage and a moving assembly; the optical reflection mechanism, the sample stage and the extraction pole piece are all arranged in the ion source cavity, the sample stage and the extraction pole piece are positioned below the optical reflection mechanism, and the moving assembly is used for moving the sample stage; the laser optical assembly, the illumination assembly and the imaging assembly are all positioned outside the ion source cavity; laser emitted by the laser optical component is reflected by the optical reflection mechanism and then is emitted to the surface of the sample; the illumination light that illumination assembly sent shines the sample surface after optical reflection mechanism reflects, the formation of image subassembly is used for receiving by the illumination light that the sample reflected is in order to be used for the sample formation of image.

When the laser mass spectrometer detects, the first motor drives the first reflector to swing back and forth around the first direction, and the second motor drives the second reflector to swing back and forth around the second direction, so that light spots of the laser can be sequentially irradiated and distributed in one detection area of a sample to be detected in a scanning mode, then the sample stage can be moved by the moving assembly of the laser mass spectrometer, and similarly, the light spots of the laser are sequentially irradiated and distributed in the other detection area of the sample to be detected in a scanning mode. Therefore, the moving assembly of the laser mass spectrometer does not need to drive the sample table at a high frequency, and the first motor and the second motor respectively drive the first reflecting mirror and the second reflecting mirror to act, so that the working requirement of the laser at the high frequency can be met, and the mass spectrometer detection and analysis efficiency can be improved.

In one embodiment, the laser mass spectrometer further includes a sample target plate disposed in the ion source cavity, the sample target plate is provided with a plurality of sample holes for placing samples, and the sample target plate is disposed on the sample stage.

In one embodiment, the laser mass spectrometer further includes a controller, and the controller is electrically connected to the laser, the first motor, the second motor, the illumination assembly, the imaging assembly, and the moving assembly respectively.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a view structural diagram of a laser optical assembly according to an embodiment of the present invention;

fig. 2 is a view of another perspective structure of a laser optical assembly according to an embodiment of the present invention;

fig. 3 is a front view of a laser optical assembly according to an embodiment of the present invention;

fig. 4 is a top view of a laser optical assembly according to an embodiment of the present invention;

fig. 5 is a side view of a laser optic assembly according to an embodiment of the present invention;

fig. 6 is a simplified schematic diagram of a laser mass spectrometer according to an embodiment of the present invention;

fig. 7 is a schematic structural diagram of a sample to be tested according to an embodiment of the present invention, for example, a tissue slice;

FIG. 8 is a schematic structural diagram of the sample to be tested illustrated in FIG. 7 divided into a plurality of detection areas;

FIG. 9 is a schematic structural diagram of one of the detection regions illustrated in FIG. 8 when laser spots are sequentially arranged;

fig. 10 is a schematic structural diagram of a sample target plate according to an embodiment of the present invention;

fig. 11 is a schematic structural view of the sample target plate shown in fig. 10 in which the area where the sample hole is located is divided into four detection areas abcd.

10. A laser optical assembly; 11. a laser; 12. a first reflector; 13. a first motor; 14. a second reflector; 15. a second motor; 16. mounting a plate; 161. a heat sink; 17. a first bracket; 18. a second bracket; 181. a light-transmitting hole; 19. a housing; 20. a lighting assembly; 30. an imaging assembly; 31. an optical lens; 32. a photographing device; 40. an ion source assembly; 41. an ion source cavity; 42. an optical reflection mechanism; 421. a reflective surface; 43. leading out a pole piece; 44. a sample stage; 45. a moving assembly; 50. a sample target plate; 51. a sample well; 60. a sample to be tested; 61. detecting a region; 70. and (4) laser spots.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Referring to fig. 1 to 5, fig. 1 illustrates a view structure diagram of a laser optical assembly 10 according to an embodiment of the present invention, fig. 2 illustrates another view structure diagram of the laser optical assembly 10 according to an embodiment of the present invention, and fig. 3 to 5 illustrate three views of the laser optical assembly 10 respectively. An embodiment of the present invention provides a laser optical assembly 10, laser optical assembly 10 includes: the laser device comprises a laser 11, a first reflector 12, a first motor 13, a second reflector 14 and a second motor 15. The laser 11 is used to emit laser light. The first reflector 12 is used for reflecting the laser emitted by the laser 11, and the first reflector 12 is connected with a power rotating shaft of a first motor 13. The power shaft of the first motor 13 is set to a first direction, and the first motor 13 is used for driving the first reflector 12 to swing around the first direction. The second reflector 14 is used for reflecting the laser reflected by the first reflector 12, the laser reflected by the second reflector 14 is used for being sent into the ion source cavity 41, and the second reflector 14 is connected with a power rotating shaft of a second motor 15. The power rotating shaft of the second motor 15 is arranged in a second direction, and the second motor 15 is used for driving the second reflecting mirror 14 to swing around the second direction. The first direction and the second direction are two different directions.

In the laser optical assembly 10, during the detection operation, the first motor 13 drives the first reflecting mirror 12 to swing back and forth around the first direction, and the second motor 15 drives the second reflecting mirror 14 to swing back and forth around the second direction, so that the light spots of the laser 11 can be sequentially irradiated and distributed on one detection area 61 of the sample 60 to be detected in a scanning manner, and then the sample stage 44 can be moved by the moving assembly 45 of the laser mass spectrometer, and similarly, the light spots of the laser 11 are sequentially irradiated and distributed on the other detection area 61 of the sample 60 to be detected in a scanning manner. Therefore, the moving assembly 45 of the laser mass spectrometer does not need to drive the sample stage 44 at a high frequency (the high frequency refers to more than 1000 Hz), but drives the first reflector 12 and the second reflector 14 to act through the first motor 13 and the second motor 15, so that the working requirement of the laser 11 at the high frequency can be met, and the mass spectrometry detection and analysis efficiency can be improved.

Specifically, the first direction is, for example, a vertical direction, that is, a direction perpendicular to the plate surface of the mounting plate 16 as in fig. 3; the second direction is, for example, a horizontal direction, that is, a direction parallel to the plate surface of the mounting plate 16 as shown in fig. 3. In this way, when the first motor 13 is used to drive the first mirror 12 to swing around the first direction, the position of the laser spot 70 (shown in fig. 9) on the x-axis direction of the detection area 61 (shown in fig. 9) can be adjusted accordingly, and when the second motor 15 is used to drive the second mirror 14 to swing around the second direction, the position of the laser spot 70 on the y-axis direction of the detection area 61 can be adjusted accordingly. When the first direction and the second direction are not perpendicular to each other, the x-axis and the y-axis are not perpendicular to each other, but are consistent with the included angle between the first direction and the second direction, and the laser light emitted by the laser 11 can be distributed in sequence over the whole detection area 61 in the form of the laser spot 70. The angle between the first direction and the second direction is not limited.

In addition, the first direction may also be arranged along a horizontal direction, and the second direction is correspondingly arranged along a vertical direction, so that the first direction and the second direction are just opposite to the first direction and the second direction in the above-mentioned embodiment, which is also a feasible solution, and is not limited herein.

Referring to fig. 1, 2 and 4, the laser optical assembly 10 further includes a mounting plate 16, a first bracket 17 and a second bracket 18. The mounting plate 16 is adapted to be mounted outside the ion source chamber 41. The first bracket 17 and the second bracket 18 are both mounted on the mounting plate 16, the first motor 13 is mounted on the first bracket 17, and the second motor 15 is mounted on the second bracket 18.

It is understood that, when the first direction is a vertical direction, the first motor 13 is installed on the first bracket 17 in a vertical manner, specifically, on the top of the first bracket 17, and may also be installed on the bottom of the first bracket 17. Similarly, when the second direction is the horizontal direction, the second motor 15 is installed on the second bracket 18 in a horizontal manner, specifically, on one side of the second bracket 18.

Further, the first support 17 and the second support 18 are formed by splicing and assembling a plurality of connecting plates or integrally formed, the connecting plates are provided with light holes 181 for avoiding laser, the light holes 181 can avoid laser beams and cannot shield the laser beams, and the laser beams are smoothly reflected to the inside of the ion cavity and hit on a sample 60 to be measured on the sample stage 44.

Referring to fig. 1, 2 and 4, further, a plurality of heat sinks 161 are disposed at intervals on a portion of the mounting board 16 corresponding to the laser 11 and on a side away from the laser 11. Specifically, the mounting plate 16 is a heat dissipating metal plate. Such as a heat dissipating aluminum plate, a heat dissipating copper plate, etc., without limitation. Therefore, heat generated in the working process of the laser 11 can be diffused outwards in time, and the normal working of the laser 11 is ensured.

Referring to fig. 1, 2 and 4, further, a power rotating shaft of the first motor 13 is perpendicular to the plate surface of the mounting plate 16, and the first reflector 12 swings with an axial direction of the power rotating shaft of the first motor 13 as a central axis. The power rotating shaft of the second motor 15 is parallel to the plate surface of the mounting plate 16, and the second reflecting mirror 14 swings with the axial direction of the power rotating shaft of the second motor 15 as a central axis. Specifically, the laser beam emitted from the laser 11 is directed parallel to the plate surface of the mounting board 16 and is directed to the first reflecting mirror 12.

Referring to fig. 1, fig. 2 and fig. 4, in an embodiment, a first included angle is formed between the first reflecting mirror 12 and the laser light emitted from the laser 11, and the first included angle is, for example, 40 ° to 50 °. In addition, a second included angle is formed between the laser beam reflected by the first reflecting mirror 12 and the second reflecting mirror 14, and the second included angle is, for example, 40 ° to 50 °. Therefore, the brightness of the laser spot 70 irradiated on the sample 60 to be detected is uniform, and the detection effect is ensured.

Further, optionally, the first mirror 12 may be swung at an angle ranging from-3 ° to 3 °, and the second mirror 14 may be swung at an angle ranging from-3 ° to 3 °. Therefore, the brightness of the laser spot 70 irradiated on the sample 60 to be detected is uniform, and the detection effect is ensured.

In one embodiment, the laser optical assembly 10 further includes a housing 19, a first focusing lens, a collimating lens and a second focusing lens, wherein the laser emitted from the laser 11 generates a divergent laser beam through the first focusing lens, the divergent laser beam becomes a collimated laser beam through the collimating lens, and the collimated laser beam is focused through the second focusing lens and can be reflected by the optical reflection mechanism 42 and then incident on the sample; the first focusing lens, the collimating lens and the second focusing lens are disposed in the housing 19, and a distance between the first focusing lens and the collimating lens is adjustable.

The housing 19 may be two housings 19, three housings 19, or a plurality of housings 19, and is not limited herein. The individual housings 19 may be movable relative to each other or may be fixed relative to each other, and are not limited herein. The position of the housing 19 alone is not limited, and may be set along the laser light path.

When the laser spot adjusting device is used, the size of the laser spot 70 can be continuously adjusted by adjusting the distance between the first focusing lens and the collimating lens, and the use is convenient.

In one embodiment, the laser 11 is a solid state laser 11 or an N2 laser 11. Preferably, the laser 11 is a solid laser 11, and compared with the N2 laser 11, the solid laser has the advantages of long service life, short pulse time, small size, high repetition rate, and the like, and is helpful for improving the resolution of the mass spectrometry detection device and reducing the maintenance frequency. Of course, the laser 11 may be another type of laser 11, and is not limited herein.

In one particular example, the laser optic assembly 10 further includes an optical filter. The filter may be, but is not limited to, a neutral density filter or the like for adjusting the energy of the laser beam. More specifically, the filter may be disposed after the second focusing lens for adjusting the energy of the laser beam focused by the second focusing lens.

Further, the laser optical assembly 10 further includes a filter adjustment mechanism. The filtering adjusting mechanism is connected with the optical filter and is used for driving the optical filter to rotate. The filtering adjusting mechanism can be a steering engine and other mechanisms, and the rotation of the filtering adjusting mechanism can drive the optical filter to rotate, so that the continuous adjustment of the laser capacity is realized.

Referring to fig. 1, fig. 2 and fig. 6, fig. 6 is a simplified schematic diagram of a laser mass spectrometer according to an embodiment of the present invention. In one embodiment, a laser mass spectrometer comprises the laser optical assembly 10 of any of the above embodiments, and further comprises an illumination assembly 20, an imaging assembly 30, and an ion source assembly 40. The ion source assembly 40 includes an ion source cavity 41, an optical reflection mechanism 42, an extraction pole piece 43, a sample stage 44, and a moving assembly 45. The optical reflection mechanism 42, the sample stage 44 and the extraction pole piece 43 are all arranged in the ion source cavity 41, the sample stage 44 and the extraction pole piece 43 are located below the optical reflection mechanism 42, and the moving assembly 45 is used for moving the sample stage 44. The laser optics assembly 10, the illumination assembly 20, and the imaging assembly 30 are all located outside the ion source cavity 41. The laser emitted from the laser optical assembly 10 is reflected by the optical reflection mechanism 42 and then emitted to the surface of the sample. The illumination light emitted from the illumination assembly 20 is reflected by the optical reflection mechanism 42 and then irradiated on the surface of the sample, and the imaging assembly 30 is used for receiving the illumination light reflected by the sample for imaging the sample.

It should be noted that the laser mass spectrometer may be a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, a laser sputtering ionization time-of-flight mass spectrometer, a single particle aerosol time-of-flight mass spectrometer, a laser ionization and inductively coupled plasma mass spectrometer, and the like, which is not limited herein.

In the laser mass spectrometer, during detection, the first motor 13 drives the first reflecting mirror 12 to swing back and forth around the first direction, and the second motor 15 drives the second reflecting mirror 14 to swing back and forth around the second direction, so that the light spots of the laser 11 can be sequentially irradiated and distributed on one detection area 61 of the sample 60 to be detected in a scanning manner, and then the sample stage 44 can be moved by the moving assembly 45 of the laser mass spectrometer, and similarly, the light spots of the laser 11 are sequentially irradiated and distributed on the other detection area 61 of the sample 60 to be detected in a scanning manner. Therefore, the moving assembly 45 of the laser mass spectrometer does not need to drive the sample stage 44 at a high frequency (the high frequency refers to more than 1000 HZ), but drives the first reflector 12 and the second reflector 14 to act through the first motor 13 and the second motor 15, so that the working requirement of the laser 11 at a high frequency can be met, and the mass spectrometry detection and analysis efficiency can be improved.

Referring to fig. 10, fig. 10 illustrates a schematic structural diagram of a sample target plate 50 according to an embodiment of the present invention, the number of sample holes 51 on the sample target plate 50 illustrated in fig. 10 is only four, for example, and other sample holes 51 are omitted and not illustrated. Further, the laser mass spectrometer further includes a sample target plate 50 disposed in the ion source chamber 41. The sample target plate 50 is provided with a plurality of sample holes 51 for placing samples, and the sample target plate 50 is arranged on the sample table 44. The number of sample holes 51 in the sample target plate 50 is not limited, and the polarity is set as required. Different types of samples 60 to be tested can be arranged in different sample holes 51 on the sample target plate 50, so that different types of samples 60 to be tested can be tested. It should be noted that the sample hole 51 is not necessarily provided in the sample target plate, and may be made of, for example, a conductive glass or a stainless steel sheet, which is also possible.

Further, the laser mass spectrometer also comprises a controller. The controller is electrically connected to the laser 11, the first motor 13, the second motor 15, the illumination assembly 20, the imaging assembly 30 and the moving assembly 45.

Further, the imaging assembly 30 is configured to receive illumination light reflected by the sample 60 to be measured. The illumination light reflected by the sample 60 to be measured and the illumination light reflected by the optical reflection mechanism 42 are symmetrically arranged with respect to a vertical line perpendicular to the sample stage 44. In one specific example, illumination light reflected by the sample is reflected by the optical reflection mechanism 42 and enters the imaging assembly 30.

More specifically, the imaging assembly 30 includes an optical lens 31 and a photographing device 32. The illumination light reflected by the optical reflection mechanism 42 is captured by the optical lens 31 and then captured by the camera 32 to be imaged. The imaging device 32 may be, for example, a CCD imaging device, and may be connected to an external display device to display an image.

As one example, the laser optics assembly 10, the illumination assembly 20, and the imaging assembly 30 are disposed around the exterior of the ion source cavity 41. The ion source cavity 41 has three transparent windows corresponding to the laser optical assembly 10, the illumination assembly 20 and the imaging assembly 30.

Further, the light source of the illumination assembly 20 is disposed opposite to the optical lens 31 of the imaging assembly 30.

Further, the laser optical assembly 10 emits laser light in a direction perpendicular to the plane defined by the illumination light reflected by the optical reflection mechanism 42 and the illumination light reflected by the sample 60.

In a specific example, the optical reflection mechanism 42 has three reflection surfaces 421 for reflecting the incident laser light to the sample to be measured 60, reflecting the illumination light emitted from the illumination assembly 20 to the sample to be measured 60, and reflecting the illumination light reflected by the sample to be measured 60 to the imaging assembly 30, respectively. As shown in fig. 6, taking the example that the illumination light reflected by the optical reflection mechanism 42 forms an angle of 5 ° with the axis perpendicular to the sample stage 44, the reflection surface 421 for reflecting the illumination light forms an angle of 42.5 ° with the incident illumination light, and similarly, the reflection surface 421 for reflecting the illumination light reflected by the sample also forms an angle of 42.5 ° with the illumination light emitted to the imaging assembly 30. Similarly, when the angle between the laser beam reflected by the optical reflection mechanism 42 and the axis perpendicular to the sample stage 44 is also 5 °, the reflection surface 421 for reflecting the laser beam also forms an angle of 42.5 ° with the incident laser beam.

Further, taking the ion source cavity 41 with a regular octahedral cylinder structure at the upper part as an example, specifically, the illumination component 20 and the imaging component 30 correspond to two opposite side walls of the octahedral cylinder structure portion of the ion source cavity 41, respectively, the laser optical component 10 corresponds to the other side wall of the octahedral cylinder structure portion of the ion source cavity 41, and a side wall is spaced between the side wall and the side wall corresponding to the illumination component 20 or the imaging component 30. The schematic diagram shown in fig. 6 does not show the laser beam path perpendicular to the plane defined by the illumination light rays reflected by the optical reflection mechanism 42 and the illumination light rays reflected by the sample.

Further, in a specific example, the optical reflection mechanism 42 is located in the middle of the ion source cavity 41, and the illumination light rays reflected by the optical reflection mechanism 42 and the illumination light rays reflected by the sample are symmetrical about the central axis of the ion source cavity 41.

In order to reduce the angle between the laser incidence direction and the center axis of the mass spectrometer flight tube as much as possible, the extraction pole piece 43 of the whole ion source assembly 40 is arranged below the optical reflection mechanism 42, and the design of the pole piece inner hole of the extraction pole piece 43 can ensure that laser and illumination light can smoothly reach the surface of a sample without being influenced by the extraction pole piece 43. Therefore, the reflected laser can reach the surface of the sample at a very small angle, ionize the sample, and the surface light source generated by illumination can reach the imaging assembly 30 through the hole array in the middle of the extraction pole piece 43.

Referring to fig. 6 to 9, fig. 7 illustrates a schematic structural diagram of a sample 60 to be measured according to an embodiment of the present invention, for example, when the sample is a tissue slice, fig. 8 illustrates a schematic structural diagram of the sample 60 to be measured illustrated in fig. 7 when the sample is divided into a plurality of detection regions 61, and fig. 9 illustrates a schematic structural diagram of one of the detection regions 61 illustrated in fig. 8 when the laser spot 70 is sequentially irradiated and arranged.

In an embodiment, a detection method of the laser mass spectrometer of any of the above embodiments includes the following steps:

placing a sample 60 to be measured on a sample stage 44;

the sample table 44 is moved by the moving assembly 45, and the sample table 44 is sequentially moved to a plurality of area positions with different positions, wherein the area positions are respectively in one-to-one correspondence with the detection areas 61 of the sample 60 to be detected;

when the sample table 44 moves to each area position, the first reflecting mirror 12 is driven to swing by the first motor 13, and the second reflecting mirror 14 is driven to swing by the second motor 15, so that the laser spots 70 irradiated by the laser 11 on the sample 60 to be detected are sequentially irradiated and distributed on the detection area 61 corresponding to the area position;

the illumination light emitted by the illumination assembly 20 is reflected by the optical reflection mechanism 42 and then irradiates the surface of the sample 60 to be measured, and the imaging assembly 30 receives the illumination light reflected by the sample 60 to be measured and images the sample 60 to be measured.

In the laser mass spectrometer, during the detection operation, the first motor 13 drives the first reflecting mirror 12 to swing back and forth in the first direction, and the second motor 15 drives the second reflecting mirror 14 to swing back and forth in the second direction, so that the light spots of the laser 11 can be sequentially irradiated and distributed on one detection area 61 of the sample 60 to be detected in a scanning manner, and then the sample stage 44 can be moved by the moving assembly 45 of the laser mass spectrometer, and similarly, the light spots of the laser 11 can be sequentially irradiated and distributed on the other detection area 61 of the sample 60 to be detected in a scanning manner. Therefore, the moving assembly 45 of the laser mass spectrometer does not need to drive the sample table 44 at a high frequency, but drives the first reflecting mirror 12 and the second reflecting mirror 14 to act through the first motor 13 and the second motor 15 respectively, so that the working requirement of the laser 11 at a high frequency can be met, and the mass spectrometer detection and analysis efficiency can be improved.

Referring to fig. 8, in one embodiment, a plurality of detection regions 61 of a sample 60 to be detected are sequentially detected in an S-shaped sequence. Thus, the detection of the plurality of detection areas 61 of the sample to be detected 60 can be realized conveniently, and the working efficiency is high. Of course, the detection regions 61 of the sample 60 may be detected in other orders, for example, the detection regions 61 of the sample 60 may be detected sequentially row by row and from left to right, which is not limited herein.

Similarly, the specific method for the laser spots 70 irradiated by the laser 11 onto the sample 60 to be detected to sequentially irradiate and spread over the detection area 61 is as follows: the laser spots 70 irradiated by the laser 11 onto the sample 60 to be detected are sequentially irradiated and distributed on the detection area 61 in an S-shaped sequence. Thus, the laser spots 70 can be quickly and efficiently distributed in the same detection area 61. Of course, the laser spots 70 may be irradiated to cover the detection area 61 in other manners, for example, the laser spots 70 may be sequentially covered in the detection area 61 in a row-by-row manner and from left to right, and the laser spots 70 may be sequentially covered in the detection area 61 in a column-by-column manner and from top to bottom, which is not limited herein.

Referring to fig. 9, fig. 9 is a schematic structural diagram illustrating a case where one of the detection regions 61 illustrated in fig. 8 is sequentially irradiated to arrange the laser spots 70. In one embodiment, the detection area 61 is a square area. Specifically, the side length of the square area is 0.5mm to 4 mm. The laser spot 70 is for example a circular or square spot with a radius adjustable from 5 μm to 1000 μm. In this embodiment, the side length of the square area illustrated in fig. 9 is specifically 1.5mm, and the laser spot 70 is specifically a circular spot with a radius of 100 μm.

Referring to fig. 7 and 8 again, in one embodiment, after the step of placing the sample 60 to be tested on the sample stage 44 and before the step of moving the sample stage 44 by the moving assembly 45, the method further includes the following steps:

the sample 60 to be detected is a tissue slice, the placement position of the sample 60 to be detected on the sample table 44 is obtained, the image information of the sample 60 to be detected is obtained, and the image information is divided into a plurality of detection areas 61; a plurality of area positions are determined based on the placement position information and the plurality of detection areas 61.

Referring to fig. 10 and 11 again, fig. 10 shows a schematic structural diagram of a sample target plate 50 according to an embodiment of the present invention, and fig. 11 shows a schematic structural diagram when the area where the sample hole 51 of the sample target plate 50 shown in fig. 10 is located is divided into four abcd detection areas 61. In one embodiment, the specific steps of placing the sample 60 to be measured on the sample stage 44 are: a sample target plate 50 is placed on the sample stage 44, and a sample 60 to be measured is placed in a sample hole 51 of the sample target plate 50. The aperture of the sample hole 51 is, for example, 3mm, the area where the sample hole 51 is located is usually divided into four detection areas 61 abcd, and the laser spots 70 that are irradiated by the laser 11 onto the sample 60 to be detected are sequentially irradiated and distributed over the detection areas 61 corresponding to the area positions.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above examples only represent some embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Claims (10)

1. A laser optic assembly, comprising:

a laser for emitting laser light;

the laser device comprises a first reflector and a first motor, wherein the first reflector is used for reflecting laser emitted by the laser device, the first reflector is connected with a power rotating shaft of the first motor, the setting direction of the power rotating shaft of the first motor is a first direction, and the first motor is used for driving the first reflector to swing around the first direction;

the second mirror and second motor, the second mirror be used for with laser after the first mirror reflection reflects, the laser that the second mirror reflected is used for sending into the ion source cavity, the second mirror with the power pivot of second motor links to each other, the power pivot of second motor sets up the direction and is the second direction, the second motor is used for the drive the second mirror winds the second direction swings, first direction with the second direction is two not equidirectionals.

2. The laser optic assembly of claim 1, further comprising a mounting plate, a first bracket, and a second bracket; the mounting plate is used for being arranged outside the ion source cavity; the first support and the second support are arranged on the mounting plate, the first motor is arranged on the first support, and the second motor is arranged on the second support.

3. The laser optical assembly according to claim 2, wherein a plurality of cooling fins are arranged on the mounting board at intervals at a position corresponding to the laser and at a side away from the laser; the mounting plate is a heat dissipation metal plate.

4. The laser optical assembly according to claim 2, wherein the power shaft of the first motor is perpendicular to the plate surface of the mounting plate, and the first mirror swings with the axial direction of the power shaft of the first motor as a central axis; the power rotating shaft of the second motor is parallel to the plate surface of the mounting plate, and the second reflector swings with the axial direction of the power rotating shaft of the second motor as a central shaft.

5. The laser optical assembly according to claim 4, wherein a first included angle is formed between the first reflecting mirror and the laser light emitted from the laser, and the first included angle is 40 ° to 50 °; a second included angle is formed between the laser reflected by the first reflector and the second reflector and is 40-50 degrees; the range of the swing angle of the first reflector is-3 degrees to 3 degrees, and the range of the swing angle of the second reflector is-3 degrees to 3 degrees.

6. The laser optical assembly according to claim 1, further comprising a housing, a first focusing lens, a collimating lens and a second focusing lens, wherein the laser emitted from the laser generates a diverging laser beam through the first focusing lens, the diverging laser beam becomes a collimated laser beam through the collimating lens, and the collimated laser beam is focused through the second focusing lens and then can be reflected by the optical reflection mechanism and then incident on the sample; the first focusing lens, the collimating lens and the second focusing lens are arranged in the shell, and the distance between the first focusing lens and the collimating lens is adjustable.

7. The laser optical assembly according to any one of claims 1 to 6, characterized in that the laser is a solid state laser or an N2 laser.

8. A laser mass spectrometer comprising the laser optic assembly of any of claims 1 to 7, further comprising an illumination assembly, an imaging assembly, and an ion source assembly; the ion source assembly comprises an ion source cavity, an optical reflection mechanism, an extraction pole piece, a sample stage and a moving assembly; the optical reflection mechanism, the sample stage and the extraction pole piece are all arranged in the ion source cavity, the sample stage and the extraction pole piece are positioned below the optical reflection mechanism, and the moving assembly is used for moving the sample stage; the laser optical assembly, the illumination assembly and the imaging assembly are all positioned outside the ion source cavity; laser emitted by the laser optical component is reflected by the optical reflection mechanism and then is emitted to the surface of the sample; the illumination light that illumination assembly sent shines the sample surface after optical reflection mechanism reflects, the formation of image subassembly is used for receiving by the illumination light that the sample reflected is in order to be used for the sample formation of image.

9. The laser mass spectrometer of claim 8, further comprising a sample target plate disposed in the ion source chamber, wherein the sample target plate has a plurality of sample holes for placing samples, and the sample target plate is disposed on the sample stage.

10. The laser mass spectrometer of claim 8, further comprising a controller electrically connected to the laser, the first motor, the second motor, the illumination assembly, the imaging assembly, and the movement assembly, respectively.

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