CN212364054U

CN212364054U - Liquid phase suspension chip detector and detection system

Info

Publication number: CN212364054U
Application number: CN202020340964.1U
Authority: CN
Inventors: 王东风; 张健
Original assignee: Shenzhen Yexin Technology Co ltd
Current assignee: Shenzhen Yexin Technology Co ltd
Priority date: 2020-03-18
Filing date: 2020-03-18
Publication date: 2021-01-15
Anticipated expiration: 2030-03-18

Abstract

The utility model provides a liquid phase suspension chip detector and detecting system, including detection room, first fluid power source, sampling needle, light source and detection device. The sampling needle communicates with the first fluid power source through the detection chamber. It is thus clear that the utility model discloses available first fluid power device is direct to carry the detection room with the sample, need not the "sample loop" of the sample of keeping in, reduces the particle loss. The sampling needle is used for sucking a sample by depending on the power provided by the first fluid power source, and sucking the cleaning liquid by depending on the power provided by the first fluid power source; the sampling needle is cleaned in a manner of sucking cleaning liquid. And what absorb the sample and absorb the washing liquid uses is the same sampling needle, and the sampling needle only needs to absorb the washing liquid just can wash the sampling needle, and simple swift realization the washing of sampling needle.

Description

Liquid phase suspension chip detector and detection system

Technical Field

The utility model relates to a biochip detects technical field, concretely relates to is used for analyzing a liquid phase suspension chip detector and detecting system.

Background

The liquid phase suspension chip technology is a top-end biomolecule detection technology integrating a current collector technology, a microparticle synthesis technology, a biomolecule hybridization technology and a high-efficiency digital signal processing technology, and the principle of the technology is that known biomolecules (DNA, RNA, polypeptide, protein and the like) are integrated on the surface of one or more microparticles to form a probe array, one or more objects to be detected in a sample are captured, one or more fluorescent substances (fluorescent dye, fluorescent group or fluorescent microparticle) coupled to the objects to be detected are marked, and then the detection is carried out by an optical method. The liquid phase suspension chip technology is used for biomolecule detection, has the obvious advantages of high precision, high flux, high speed, low cost and the like, and is a novel biomolecule detection technology.

The traditional detection method is a flow cytometer method, which is used for analyzing the optical characteristics of the particles and the object to be detected by measuring the internally or externally dyed particles coupled with fluorescent materials such as fluorescent dyes, fluorescent groups and the like, so as to reflect the information of the index to be detected. The same product can refer to Luminex200 of Luminex company in America, and the biomolecule detection effect with high sensitivity and strong specificity can be obtained. Flow cytometers, however, include a number of relatively delicate and expensive devices such as lasers, high precision syringe pumps, photomultiplier tubes (PMTs), avalanche photodiodes, and the like, which, while superior in performance, are relatively costly. In addition, flow cytometers require more sheath fluid to operate properly, and the instruments are relatively large, heavy, fragile, and complex in construction, requiring specialized technicians to install them.

For the above reasons, the skilled person will gradually note that fluorescence imaging techniques can also be applied to biomolecule detection techniques, and that such systems can classify cells or particles by means of images generated using light of different wavelengths. A system and method for performing measurements of one or more materials using fluorescence imaging technology has been developed, such as described in patent CN101479603B to wien D rayne et al, which has many advantages over systems used in flow cytometry, such as cheaper price, simpler optical configuration, more stable mechanical properties, smaller volume, higher detection sensitivity, less sheath fluid usage, etc. In the preferred form of this patent, as shown in fig. 1, the fluid handling subsystem includes a sample container 180, a sample loop 140, an imaging chamber 120, two valves (130 and 160), a pump 170, etc., which requires the sample loop 140 to temporarily store the sample, the presence of the sample loop 140 lengthens the tubing required to drive the sample into the imaging chamber, the draw flow is complicated, and the longer tubing results in greater particle loss due to the partial retention of the particles to be analyzed on the tube wall or in the tubing during the process of driving the sample into the imaging chamber, which in turn affects the accuracy of the test results. This patent also provides an alternative fluid path, as shown in fig. 2, which is cost effective without the sample loop 140, but which does not allow the sample probe to be cleaned with a washing fluid, resulting in one sample being tested and the next sample being tested, with the previous sample remaining in the sample probe and interfering with the test results.

SUMMERY OF THE UTILITY MODEL

The application provides liquid phase suspension chip detector and detecting system aims at being convenient for clean, avoids producing the interference to the testing result.

According to a first aspect, there is provided in an embodiment a detection system for analysing one or more particles, comprising:

a detection chamber for providing a detection site for one or more particles to be analysed;

a first fluid power source for powering a flow of liquid in the fluid path;

the sampling needle is used for sucking a sample depending on the power provided by the first fluid power source and sucking the cleaning liquid depending on the power provided by the first fluid power source; wherein the sampling needle is cleaned in a manner of sucking cleaning liquid; the sampling needle is in communication with a first fluid power source through the detection chamber;

a light source for illuminating the particles within the detection chamber to cause the particles to emit light signals related to the characteristics of the particles themselves;

and the detection device is used for detecting the optical signals of the particles in the detection chamber.

According to a second aspect, there is provided in an embodiment a detection system for analysing one or more particles, comprising:

a sample suction station for providing a sample to be analysed, the sample comprising one or more particles to be analysed;

a liquid suction level for placing a cleaning liquid container to provide a cleaning liquid;

a first fluid power source for powering a flow of liquid in the fluid path;

the sampling needle is used for sucking a sample by depending on power provided by the first fluid power source when the sampling position is reached, and sucking cleaning liquid by depending on power provided by the first fluid power source when the sampling position is reached, wherein the sampling needle is used for cleaning the sampling needle in a manner of sucking the cleaning liquid; the sampling needle is in communication with a first fluid power source through the detection chamber;

the driving device is used for driving the sampling needle and/or the sample sucking position to move so that the sampling needle is positioned at the sample sucking position when sucking samples, and is used for driving the sampling needle and/or the liquid sucking position to move so that the sampling needle is positioned at the liquid sucking position when sucking cleaning liquid;

According to a third aspect, there is provided in one embodiment a detection system for analysing one or more particles, comprising:

a first fluid power source for powering a flow of liquid in the fluid path; a first fluid power source in communication with the outlet of the detection chamber;

a second fluid power source for powering the flow of liquid in the fluid path;

the sampling needle is used for sucking a sample depending on power provided by the first fluid power source and sucking cleaning liquid depending on power provided by the first fluid power source or the second fluid power source, wherein the sampling needle is used for cleaning the sampling needle in a manner that the second fluid power source sucks the cleaning liquid;

the first gating device is used for selective communication, and the inlet of the detection chamber and the second fluid power source are both connected with the sampling needle through the first gating device;

According to a fourth aspect, there is provided in an embodiment a detection system for analysing one or more particles, comprising:

a second fluid power source for powering the flow of liquid in the fluid path;

the sampling needle is used for sucking a sample by depending on power provided by the first fluid power source when the sampling position is reached and sucking cleaning liquid by depending on power provided by the first fluid power source or the second fluid power source when the sampling position is reached, wherein the sampling needle is used for cleaning the sampling needle in a manner that the second fluid power source sucks the cleaning liquid;

According to a fifth aspect, there is provided in an embodiment a detection system for analysing one or more particles, comprising:

a second fluid power source for powering the flow of liquid in the fluid path;

the three-way device is provided with a first interface, a second interface and a third interface, and the first interface, the second interface and the third interface are communicated with each other; a first interface of the three-way device is connected with an inlet of the detection chamber, a second interface of the three-way device is connected with the sampling needle, and a third interface of the three-way device is connected with a second fluid power source;

According to a sixth aspect, an embodiment provides a liquid-phase suspension chip detector comprising a detection system as described above for analyzing one or more particles.

The liquid phase suspension chip detector and the detection system according to the embodiment comprise a detection chamber, a first fluid power source, a sampling needle, a light source and a detection device. The sampling needle communicates with the first fluid power source through the detection chamber. It is thus clear that the utility model discloses available first fluid power device is direct to carry the detection room with the sample, need not the "sample loop" of the sample of keeping in, reduces the particle loss. The sampling needle is used for sucking a sample by depending on the power provided by the first fluid power source, and sucking the cleaning liquid by depending on the power provided by the first fluid power source; the sampling needle is cleaned in a manner of sucking cleaning liquid. And what absorb the sample and absorb the washing liquid uses is the same sampling needle, and the sampling needle only needs to absorb the washing liquid just can wash the sampling needle, and simple swift realization the washing of sampling needle.

Drawings

FIG. 1 is a block diagram of a fluid path structure of a conventional detection system;

FIG. 2 is a block diagram of another fluid path structure in a prior art detection system;

FIG. 3 is a block diagram of an embodiment of a detection system for analyzing one or more particles provided by the present invention;

FIG. 4 is a block diagram of another embodiment of a detection system for analyzing one or more particles provided by the present invention;

FIG. 5 is a schematic diagram of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

FIG. 6 is a block diagram of a first embodiment of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

FIG. 7 is a block diagram of a second embodiment of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

fig. 8 is a block diagram of a third embodiment of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

fig. 9 is a block diagram of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

FIG. 10 is a block diagram of the fifth and seventh embodiments of the fluid path subsystem of the detection system for analyzing one or more particles provided by the present invention;

fig. 11 is a schematic diagram of a fluid path subsystem employing a second fluid power source to clean a sampling needle in a detection system for analyzing one or more particles according to the present invention;

fig. 12 is a block diagram of a sixth embodiment of a fluid path subsystem in a detection system for analyzing one or more particles according to the present invention;

FIG. 13 is a block diagram of an embodiment of an optical subsystem in a detection system for analyzing one or more particles according to the present invention;

FIG. 14 is a flow chart of a detection method in a detection system for analyzing one or more particles according to the present invention;

FIG. 15 is a block diagram of an embodiment of a second gating device in a detection system for analyzing one or more particles according to the present invention;

FIG. 16 is a block diagram of another embodiment of a second gating device in a detection system for analyzing one or more particles according to the present invention;

fig. 17 is another schematic diagram of a fluid path subsystem employing a second fluid power source to clean a sampling needle in a detection system for analyzing one or more particles according to the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

In the prior art shown in fig. 1, a sample needs to be sucked and transferred to the sample loop 140 for temporary storage through the cooperation of a valve and a pump, and then the state of the valve is changed to change the action direction of the pump on the liquid, so as to transfer the sample in the sample loop 140 to the imaging chamber 120, the presence of the sample loop 140 not only complicates the flow of transferring the sample to the imaging chamber, but also lengthens the pipeline required for driving the sample to the imaging chamber, and as the particles to be analyzed partially remain on the pipe wall or in the pipeline in the process of driving the sample, the longer pipeline causes more loss of the particles, thereby affecting the accuracy of the detection result. Whereas in the prior art shown in fig. 2, the pump 170 is a one-way pump, the tubing or probe between the valve 190 and the sample container 180 cannot be cleaned. And the utility model discloses an optimize the liquid way structure, can not only make the sample directly get into the detection room, need not to keep in, be convenient for wash the probe moreover, see below in concrete scheme.

The utility model provides a detecting system for analyzing one or more particle, it is applicable in liquid phase suspension chip detector (also known as liquid phase biochip detector, perhaps liquid phase chip detector), of course, also can be applicable to other instruments that need to analyze one or more particle. As shown in fig. 3, the detection system includes: a controller 1, an optical path subsystem 2, a liquid path subsystem 3 and a driving subsystem 5. The controller 1 effects detection of one or more particles by controlling the optical path subsystem 2, the fluid path subsystem 3, and the driver subsystem 5. Referring to fig. 5, the fluid path subsystem at least includes: a sampling needle a, a detection chamber 33 and a first fluid power source 31. The sampling needle a communicates with the first fluid power source 31 through the detection chamber 33.

In operation, the user may manually load, apply the cleaning solution, e.g., manually place the sample container 36 under the sampling needle a, manually place the cleaning solution container 37 under the sampling needle a, etc. Of course, the operation can be performed automatically, the manual mode mainly includes that a user replaces a driving device manually, a sample sucking level and a waste discharging level are not needed, other processes are the same as the automatic mode, and the manual mode and the automatic mode are respectively described in detail below.

The sample container 36 holds a sample to be analyzed, and in particular, the sample is held in the sample container 36 in a liquid form, and the sample contains one or more particles to be analyzed. Wherein, the particle can be for combining the particle that has the analyte and have the fluorescent substance sign, and the different then kind of particle of analyte is just different usually, the utility model discloses can detect one or more analyte. The analyte may be a biomolecule or the like. The particles may be encoded by particle shape, particle pattern (e.g., any pattern may be etched on the particle surface), particle size, fluorescence or raman spectroscopy, etc. The particles may range in size from nanometers to micrometers and may be microparticles of various shapes, for example, the particles may be microspheres having a particle size in the range from nanometers to micrometers. The analyte is usually associated with a fluorescent substance, for example, the fluorescent substance is used to identify the presence or quantity of the analyte, and in some embodiments, a fluorescent substance is associated with an analyte, and its corresponding analyte is identified by its specific fluorescence. The fluorescent substance is typically a fluorescent dye, a quantum dot, an up-conversion luminescent material, or the like. The fluorescent substance is capable of emitting fluorescence upon excitation with light.

The cleaning liquid container 37 stores a cleaning liquid. The washing liquid plays the effect of drive and washing in the liquid way subsystem, and all can play the liquid of drive and washing effect, for example, liquid such as phosphate buffer solution, carbonate buffer solution, ammonium salt buffer solution, deionized water, pure water, the utility model discloses do not do the injecion.

The detection chamber 33 is used to provide a detection site for one or more particles to be analyzed, e.g., to provide space for a monolayer arrangement of particles in a sample, to facilitate detection of the particles within the detection chamber 33 by the optical path subsystem 2. Generally, the detection of the particles by the optical subsystem 2 is imaging, and brightness, quantity, etc. are identified according to the image formed by imaging. Thus, the detection chamber 33 is typically an imaging chamber. The sample particles in the detection chamber 33 are arranged in a monolayer under the action of gravity, magnetic force or electric field force to form a detection plane. The controller 1 may control the first fluid power source 31 to align the particles in the sample in a single layer in the detection chamber 33 by gravity, or may generate a magnetic force or an electric force by a specific device to restrain the particles in the detection chamber 33 to align the particles in a single layer. The detection chamber 33 is at least front transparent to facilitate particle detection, e.g., imaging, by the optical path subsystem 2 through the front of the detection chamber 33. The back of the detection chamber 33 may be transparent or opaque.

The first fluid power source 31 is for supplying power to the flow of the liquid in the liquid path, and operates under the control of the controller 1 to supply power to the flow of the liquid in the liquid path, for example, to suck a sample, a cleaning liquid, and the like into the liquid path and to discharge the liquid path. The first fluid power source 31 is a device capable of driving a flow of liquid in a conduit, such as various types of pumps or combinations of multiple pumps connected by tubing, valves, or the like. The controller 1 is electrically connected to the first fluid power source 31 to control the operation of the first fluid power source 31. The fluid path subsystem is substantially sealed to enable the first fluid power source 31 to draw fluid through the fluid path, drive fluid flow to the corresponding location, and expel fluid.

The sampling needle a is used for sucking a sample depending on the power supplied by the first fluid power source 31 and sucking a cleaning liquid depending on the power supplied by the first fluid power source 31.

As shown in fig. 13, the optical path subsystem 2 includes a light source (not shown) and a detection device 21. The controller 1 is electrically connected to the light source and detection device 21, and controls the light path subsystem 2 by controlling the light source and detection device 21. The light source is used to illuminate the particles f in the detection chamber 33 so that the particles f emit light signals, such as fluorescent light, that are related to the properties of the particles themselves. The detection device 21 detects an optical signal of the particle f in the detection chamber 33. The controller 1 controls the liquid path subsystem 3, the drive subsystem 5, the light source, and the detection device 21 to detect the optical signal of the particle f in the detection chamber 33. Since the particles have a fluorescent substance, the fluorescent substance is excited under irradiation of the light source, thereby emitting fluorescence. Further, the light intensity (emission intensity) of the fluorescence of the particles f in the detection chamber 33 is detected by the detection device 21. The detection device 21 may detect the fluorescence of the particles f in the detection chamber 33 when the particles f in the detection chamber 33 are irradiated with the light source, or may detect the fluorescence of the particles f in the detection chamber 33 after the particles f in the detection chamber 33 are irradiated with the light source (for example, the upconversion luminescent material has a long fluorescence lifetime and the particles still emit fluorescence for a while after the irradiation with the light source is stopped). For example, imaging and detecting the particles arranged in a single layer (for example, detecting pixel values of pixel points of the particles, etc.) to obtain light intensity; the utility model discloses do not do the restriction.

The controller 1 is used to control the optical subsystem 2, the liquid subsystem 3 and the driving subsystem 5 to implement the detection of one or more particles, for example, a user manually places the sample container 36 under the sampling needle a and makes the sampling needle a be under the liquid level, and then the controller 1 controls the first fluid power source 31 to provide power to make the sampling needle a suck the sample in the sample container 36 and deliver the sucked sample to the detection chamber 33. After the sample aspiration, the user manually places the cleaning liquid container 37 under the sampling needle a and puts the sampling needle a under the liquid level; the controller 1 controls the first fluid power source 31 to provide power to make the sampling needle a suck cleaning liquid to clean the sampling needle a. Preferably, after the sample suction and before the detection, the controller 1 controls the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning liquid to clean the sampling needle a; of course, the controller 1 may control the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning solution to clean the sampling needle a after the detection is completed. That is, after the sample suction, the user can remove the sample container 36 to connect the cleaning solution container 37 to the sampling needle a, and thereafter, the sampling needle a can be caused to suck the cleaning solution to clean the sampling needle a for a period of time other than a period in which the cleaning solution cannot be sucked when the particles in the detection chamber 33 are detected. After the sample is sucked, the sampling needle a usually sucks the sample which meets the required dosage of detection.

The above process is a manual sample loading process for a user, and of course, the sample loading process may also be automatic, which is described in detail below. As shown in fig. 5, the utility model provides a detection system still includes: a sample suction position b and a liquid suction position d.

The sample pick-up location b refers to the location where the sample is picked up by the sampling needle a, and in one embodiment, may be a fixed device, such as a recessed slot, for receiving the sample container 36 to provide the sample to be analyzed, and may also be a container itself for holding the sample to be analyzed. In some embodiments, the sample sucking position b may also be a coordinate position, and when the sample needs to be sucked, the sample sucking position b is moved to the coordinate position by the sampling needle and/or the sample container to realize the collection of the sample.

The suction level d refers to a position where the sampling needle a sucks the cleaning solution, and in one embodiment, the position may be a fixed facility, such as a recessed slot, for placing the cleaning solution container 37 to supply the cleaning solution, and the suction level d may also be a container for containing the cleaning solution itself. In some embodiments, the liquid sucking position d may also be a coordinate position, and when the cleaning liquid needs to be sucked, the sampling needle and/or the cleaning liquid container move to the coordinate position to suck the cleaning liquid.

The drive subsystem 5 comprises a drive means (not shown in the figures). The controller 1 is electrically connected with the driving device to control the driving device to drive.

The driving device is used for driving the sampling needle a and/or the sample sucking position b to move, so that the sampling needle a is positioned at the sample sucking position during sample sucking, and a sample is sucked from the sample container 36 on the sample sucking position. The sampling needle a is located at the pipetting site, in particular at a position adapted to the sample container 36, in which position the sampling needle a can aspirate a sample. The driving device has three ways to make the sampling needle a be positioned at the sample sucking position when sucking the sample. The first of the three ways: the sample sucking position b does not move, and the driving device only drives the sampling needle a to move, so that the sampling needle a is positioned at the sample sucking position during sample sucking. Second of the three ways: the sampling needle a does not move, and the driving device only drives the sample sucking position b to move, so that the sampling needle a is positioned at the sample sucking position during sample sucking. Third of the three ways: the driving device drives the sampling needle a and the sample sucking position b to move, so that the sampling needle a is positioned at the sample sucking position during sample sucking; for example, the driving device drives the sample suction position to move below the sampling needle a, drives the sampling needle a to move downwards, makes the sample suction end of the sampling needle a extend into the sample in the sample container 36 on the sample suction position b, makes the inlet of the detection chamber 33 communicate with the sample container 36, and then the first fluid power source 31 generates power to suck the sample into the liquid path.

The driving device is also used for driving the sampling needle a and/or the liquid suction position d to move, so that the sampling needle a is positioned at the liquid suction position d when sucking the cleaning liquid, and the cleaning liquid is conveniently sucked from the cleaning liquid container 37 on the liquid suction position. The sampling needle a is located at the liquid suction position, specifically, at a position fitted with the cleaning liquid container 37, at which the sampling needle a can suck the cleaning liquid. The driving device has three modes to make the sampling needle a be positioned at the liquid sucking level when sucking liquid (sucking cleaning liquid). The first of the three ways: the liquid suction level d does not move, and the driving device only drives the sampling needle a to move, so that the sampling needle a is positioned at the liquid suction level during liquid suction. Second of the three ways: the sampling needle a does not move, and the driving device only drives the liquid absorption level d to move, so that the sampling needle a is positioned at the liquid absorption level during liquid absorption. Third of the three ways: the driving device drives the sampling needle a and the liquid suction position d to move, so that the sampling needle a is positioned at the liquid suction position during liquid suction; for example, the driving device drives the suction level to move below the sampling needle a, drives the sampling needle a to move downwards, makes the sample suction end of the sampling needle a extend into the cleaning liquid in the cleaning liquid container 37 on the suction level d, makes the inlet of the detection chamber 33 communicate with the cleaning liquid container 37, and then the first fluid power source 31 generates power to suck the cleaning liquid into the liquid path.

Wherein the sample sucking position b and the liquid sucking position d can be different positions, for example, the driving subsystem 5 further comprises a tray, one position of the tray is used for placing the sample container 36, the sample sucking position b is used for placing the cleaning liquid container 37, the other position of the tray is used for placing the cleaning liquid container d, and the driving device moves the sampling needle a and/or the tray so that the sampling needle a is positioned at the sample sucking position during sample sucking and the sampling needle a is positioned at the liquid sucking position during liquid sucking. Of course, the sample suction position b and the liquid suction position d may be the same position, for example, the driving subsystem 5 further includes a tray on which a position for placing both the sample container 36 and the cleaning solution container 37 is placed, the position being the sample suction position b when the sample container 36 is placed thereon, and the position being the liquid suction position d when the cleaning solution container 37 is placed thereon.

The controller 1 is used for controlling the optical subsystem 2, the liquid subsystem 3 and the driving subsystem 5 to implement the detection of one or more particles, and the process thereof is as shown in fig. 14, and at least includes the following steps:

in the sample sucking step S2, the controller 1 controls the operation of the first fluid power source 31 and controls the driving of the driving device, when the controller 1 controls the driving device to position the sampling needle a at the sample sucking position, the sampling needle a communicates with the first fluid power source 31 through the detection chamber 33, and controls the first fluid power source 31 to provide power to make the sampling needle a suck the sample at the sample sucking position b and deliver the sucked sample to the detection chamber 33, in this embodiment, the sample container 36 placed at the sample sucking position b is taken as an example for explanation, that is, the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the sample from the sample container 36 placed at the sample sucking position b and deliver the sucked sample to the detection chamber 33. The sample sucked by the sampling needle a is the sample to be detected. It can be seen that the sample drawn by the sampling needle a can be directly sucked into the detection chamber 33, a sample loop for temporarily storing the sample is not needed, and the liquid path through which the sample passes is short, so that the particle loss is small. When the sampling needle a is positioned at the sample sucking position, the sampling needle a is communicated with the first fluid power source 31 through the detection chamber 33, namely, the sampling needle a is communicated with the detection chamber 33 and the first fluid power source 31 in sequence in an embodiment without the first gating device, and the first gating device is controlled to be communicated with the first fluid power source 31 through the detection chamber 33 in an embodiment with the first gating device. Similarly, after the sample is sucked, the controller controls the driving device to enable the sampling needle to be located at the sucking liquid level, the sampling needle is communicated with the first fluid power source through the detection chamber, and the first fluid power source is controlled to provide power to enable the sampling needle to suck the cleaning liquid on the sucking liquid level so as to clean the sampling needle. The sampling needle a can be cleaned at two preferable occasions, wherein one of the preferable occasions is that after the sample is sucked and before the detection, the sampling needle a is communicated with the first fluid power source 31 through the detection chamber 33, and the controller 1 controls the first fluid power source 31 to provide power to make the sampling needle a suck cleaning liquid at the liquid suction position d so as to clean the sampling needle. Of course, before the sampling needle a sucks the cleaning solution on the liquid suction position d to clean the sampling needle, the controller 1 controls the driving device to make the sampling needle a be located at the liquid suction position d, and the operation is performed after the sample is sucked and before the cleaning solution is sucked to clean the sampling needle.

And a detection step S3, wherein the controller 1 controls the optical path subsystem 2 to detect the sample to be detected in the detection chamber. Another timing for cleaning the sampling needle a is after the detection is completed, in other words, after the detection is completed, the sampling needle a is communicated with the first fluid power source 31 through the detection chamber 33, and the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the cleaning liquid at the liquid suction position d to clean the sampling needle. Since the controller 1 controls the driving device to make the sampling needle a located at the liquid suction level d, which is performed after the sample is sucked and before the cleaning liquid is sucked to clean the sampling needle, the controller 1 may control the driving device to make the sampling needle a located at the liquid suction level d after the sample is sucked, before the detection, during the detection, or after the detection is completed, and then control the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning liquid at the liquid suction level d to clean the sampling needle.

Of course, in order to clean more thoroughly, the sampling needle a can be cleaned once after the sample is sucked and before the detection, and the sampling needle a can be cleaned once after the detection is finished.

Further, sampling needle a absorbs the washing liquid in order to wash the sampling needle on imbibition position d, and sampling needle a specifically washs the sampling needle with the mode of absorbing the washing liquid on imbibition position d, promptly, after sampling needle a absorbs the sample, has the sample to remain in its pipe wall, and when follow-up sampling needle a absorbs the washing liquid, the washing liquid will be taken away remaining sample, has accomplished the washing.

Because the sample sucking and the cleaning liquid sucking are both the same sampling needle a, the cleaning liquid sucking is just to clean the sampling needle a, and the cleaning process is simple and efficient. The sampling needle a may be a tube with a needle head at one end, or a needle head, or a tube, etc., as long as it can suck a sample and a cleaning liquid, the utility model discloses do not do the limitation. The sampling needle a is located at the suction level d and is communicated with the first fluid power source 31 through the detection chamber 33 in the same way as in the case of the embodiment without the first gating means, the sampling needle a itself is communicated with the detection chamber 33 and the first fluid power source 31 in turn, and in the case of the embodiment with the first gating means, the first gating means is controlled to communicate the sampling needle a with the first fluid power source 31 through the detection chamber 33.

Further, in the schematic diagram shown in fig. 5, the first fluid power source 31 communicates with the outlet of the detection chamber 33, and may be directly connected to the outlet of the detection chamber 33 or connected to the outlet of the detection chamber 33 through the first pipe g1, and the present invention is described by taking as an example the case where the first fluid power source 31 is connected to the outlet of the detection chamber 33 through the first pipe g 1.

FIG. 5 is a schematic block diagram of a fluid path subsystem, and the fluid path subsystem may take various forms, as shown in FIGS. 6-10, wherein the direction of the arrows in FIGS. 6-10 indicate the direction of fluid flow, and the single arrow indicates that only one-way flow is possible; the double arrows indicate that flow is possible in both directions, i.e. one time may flow in one direction and another time may flow in the other direction. The five-fluid-path subsystems shown in fig. 6-10 are described as five embodiments one by one.

In the first embodiment (the embodiment of the fluid path subsystem shown in fig. 6), in this embodiment, a first gating device 34 is added to the fluid path subsystem based on the fluid path subsystem shown in fig. 5. In this embodiment, the first gate 34 has an outlet 1, an inlet 2, and a drain 3. The first gating means 34 gates the detection chamber 33, the sampling needle a, and the liquid discharge port 3, specifically, for switching to communicate the outlet 1 with the inlet 2; the outlet 1 is switched to communicate with the drain port 3. The outlet 1 of the first gating means 34 is connected to the inlet of the detection chamber 33 directly or through a tube, and the inlet 2 of the first gating means 34 is connected to the sampling needle a. The drain port 3 of the first gate 34 is used for draining the waste liquid toward the waste liquid recovery device 38, for example, the drain port 3 directly discharges the waste liquid to the waste liquid recovery device 38, or the drain port 3 communicates with the waste liquid recovery device 38 through the second pipe g2 and discharges the waste liquid to the waste liquid recovery device 38 through the second pipe g 2. The first fluid power source 31 is connected to the outlet of the detection chamber 33 by a first conduit g 1. The connection on the liquid path of the utility model can be a direct connection and can also be connected through a pipe.

The waste fluid recovery device 38 may be a section of tube or any other suitable container configuration that can receive waste fluid, and the received waste fluid may be specifically received waste fluid or received waste fluid that is discharged. The waste liquid may be a sample subjected to detection or a cleaning liquid.

The first gating means 34 selects one of the sampling needle a and the liquid discharge port 3 to communicate with the inlet of the detection chamber 33, that is, one of the sampling needle a and the liquid discharge port 3 to communicate with the inlet of the detection chamber 33, and the other to not communicate with the inlet of the detection chamber 33, based on the control of the controller 1. The first gating means 34 may comprise: one or more pipes, and one or more valves. The valve in the present invention may be any suitable valve known in the art. In the present embodiment, the first gating device 34 includes a first valve J, which includes three joints for liquid to flow in and out, and these three joints are respectively used as the outlet 1, the inlet 2 and the liquid outlet 3 of the first gating device 34. The joint 1 of the first valve J is connected to the inlet of the sensing chamber 33, the joint 2 of the first valve J is connected to one end of the sampling needle a, and the joint 3 of the first valve J is connected to the second pipe g 2. The second pipe g2 may be various types of pipes, but the present invention is not limited thereto.

In this embodiment, the controller 1 controls the optical path subsystem 2, the liquid path subsystem 3 and the driving subsystem 5 to detect one or more kinds of particles, and includes the following steps:

in the preprocessing step S1.1, the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, and controls the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning liquid from the cleaning liquid container 37 until the whole liquid path is filled with the cleaning liquid. For example, the entire fluid path shown in fig. 6 is sealed, the first fluid power source 31 includes a pump, the pump is in a pumping operation, the cleaning fluid is sucked into the fluid path, the pump continues to operate to fill the entire fluid path with the cleaning fluid, and the pump further has a continuous pumping capability for subsequent pumping of the sample. The whole liquid path is filled with the cleaning liquid before the sample suction, so that bubbles in the liquid path can be avoided, and the interference of the bubbles on the imaging of the detection chamber 33 is avoided. Of course, it is not necessary to fill the entire liquid path with the wash liquid prior to aspiration. The controller 1 controls the first gating means 34 to connect the inlet of the detection chamber 33 with the sampling needle a, and controls the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning liquid from the cleaning liquid container 37, wherein the sucked cleaning liquid is used for cleaning the detection chamber 33 and/or the related liquid path subsequently, and the sucked cleaning liquid is at least required to be in an amount required for cleaning the detection chamber 33 and/or the related liquid path after particle detection.

And a sample sucking step S2.1, wherein the controller 1 controls the driving device to enable the sampling needle a to be located at a sample sucking position, and the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a. In this way, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected in this order. And in turn controls the first fluid power source 31 to provide power to cause the sampling needle a to draw a sample from a sample container 36 placed at the draw position b and deliver the drawn sample to the detection chamber 33. The amount of sample aspirated depends on the needs of the assay. The utility model discloses a sample directly gets into detection room 33 from sample container 36, need not to keep in the sample loop of prior art temporarily, therefore not only the simple efficient of process, the particle of pipeline loss is few moreover.

The particles in the sample may be arranged in a monolayer within the detection chamber 33 by the action of gravity, or may be constrained in a monolayer arrangement by a particle constraining means, the latter being exemplified in the present embodiment. As shown in fig. 4 and 13, in the present embodiment, the detection system further includes a particle confinement device 4, and the controller 1 is electrically connected to the particle confinement device 4; the particles are magnetic particles f combined with an analyte and marked by fluorescent substances; the particle confinement means 4 is for magnetically confining the particles f in the detection chamber 33 to a monolayer arrangement under the control of the controller 1, and magnetically fixing the particles f in the detection chamber 33. The particle restraining device 4 can move, the controller 1 controls the particle restraining device 4 to move to the lower part of the detection chamber 33, then the particle restraining device 4 restrains and fixes the particles f in the detection chamber 33 through magnetic force, and the controller 1 controls the particle restraining device 4 to move away, then the particles f are not restrained by the magnetic force. The particle confinement means 4 comprises a magnet and moving means for driving the magnet to move to the back of the detection chamber 33 and for driving the magnet to move away. The magnets may be various types of magnets, such as permanent magnets, electromagnets, and the like. Of course, in other embodiments, the particle confinement device 4 may not confine the particle f by magnetic force, but may instead confine the particle f to a monolayer arrangement by electric force, for example, the particle confinement device 4 may first make the particle f charged, and then confine the particle f in the detection chamber 33 to a monolayer arrangement by electric force, and fix the particle f in the detection chamber 33 by electric force.

In this step, after the sample is aspirated, the transfer of the aspirated sample to the detection chamber 33 may be specifically realized in four ways. This is described below.

In the first mode, the controller 1 controls the driving device to position the sampling needle a at the suction level, the inlet of the detection chamber 33 communicates with the cleaning liquid container 37, and controls the first fluid power source 31 to operate to suck the cleaning liquid. In this way, a liquid flow is formed in the liquid path, in which the washing liquid sucked in step S1.1 is in front, the sample sucked in this step is in the middle, and the washing liquid sucked in this step is in the rear. Therefore, the cleaning liquid is arranged in the front and at the back of the sample in the liquid path, and bubbles are avoided. The controller 1 controls the first fluid power source 31 to deliver the liquid flow, the cleaning liquid in the liquid flow passes through the detection chamber 33 (if the detection chamber 33 is not cleaned, the cleaning function can also be performed), and before the sample enters the detection chamber 33, the controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33 to generate the magnetic field. The first fluid power source 31 continues to suck the liquid, the sample enters the detection chamber 33, the particles f in the sample are arranged and fixed in a single layer under the action of the magnetic field, at this time, the first fluid power source 31 can stop working, preferably, the first fluid power source 31 stops working after continuously sucking the liquid, so that part of the cleaning liquid behind the sample flows through the detection chamber 33 to complete the washing of the particles f (the cleaning liquid flows through the detection chamber 33 to carry away the impurities except the particles f, and the particles f are fixed by the magnetic force and cannot be carried away). Since the capacity, length, cross-sectional area, etc. of the various components of the fluid path subsystem, and the piping are known, the rate at which the first fluid power source 31 draws (pumps) and outputs (pumps) fluid is known, and controlling the operating speed and operating time of the first fluid power source 31 delivers fluid to the desired location. In this mode, the washing liquid that absorbs after the appearance of inhaling can be in the same direction as taking washing sampling needle a, and is very convenient.

Second, the controller 1 controls the first fluid power source 31 to deliver the aspirated sample such that a portion of the sample is located to the left of the outlet of the detection chamber 33 (within the first duct g1, and/or within the first fluid power source 31), a portion of the sample is located between the outlet and the inlet of the detection chamber 33 (i.e., the detection chamber 33 is full of the sample), and another portion of the sample is located to the right of the detection chamber 33 (e.g., within the duct between the sampling needle a and/or the sampling needle a and the inlet of the detection chamber 33). In other words, the controller 1 controls the first fluid power source to deliver the fluid flow formed by the cleaning solution sucked in step S1.1 and the sample sucked in this step, so that the cleaning solution sucked in step S1.1 and a part of the sample sucked in step S2.1 flow through the detection chamber 33, a part of the sample sucked in step S2.1 fills the detection chamber 33, and another part of the sample sucked in step S2.1 is located on the right side of the detection chamber 33 (for example, in the pipeline between the sampling needle a and/or the sampling needle a and the inlet of the detection chamber 33). Similarly, before the sample enters the detection chamber 33, the controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33, and generates the magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field. Thus, in contrast to the first mode, the cleaning solution is not sucked up after the sample, and the detection chamber 33 is filled with the liquid and no bubble is generated. As for the cleaning of the sampling needle a, it can be realized by sucking the cleaning liquid after the completion of the detection.

In a third mode, the controller 1 controls the first fluid power source 31 to deliver the sucked sample to the detection chamber 33; the detection chamber 33 is protected from air bubbles by the first fluid power source 31 back delivering a portion of the cleaning fluid that flows through the detection chamber 33. In other words, the controller 1 controls the first fluid power source 31 to deliver the fluid flow formed by the cleaning solution sucked in step S1.1 and the sample sucked in this step, so that the cleaning solution sucked in step S1.1 flows through the detection chamber 33 and the sample is located in the detection chamber 33; the fluid flow is then reversed (i.e., pumped out) by the first fluid power source 31 to avoid bubbling in the detection chamber 33. Similarly, before the sample enters the detection chamber 33, the controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33, and generates the magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field. As for the cleaning of the sampling needle a, it can be realized by sucking the cleaning liquid after the completion of the detection.

In a fourth mode, the controller 1 controls the first fluid power source 31 to deliver the sucked sample to the detection chamber 33. Similarly, before the sample enters the detection chamber 33, the controller 1 controls the moving device to drive the magnet to move to the back of the detection chamber 33, and generates the magnetic field. After the sample enters the detection chamber 33, the particles f in the sample are arranged in a single layer and fixed under the action of the magnetic field. As for the cleaning of the sampling needle a, it can be realized by sucking the cleaning liquid after the completion of the detection.

In the detection step S3.1, the controller 1 controls the detection device 21 to detect the optical signal of the particle f in the detection chamber 33. As shown in fig. 13, the optical path subsystem 2 further includes a filter switching device 22. The filter switching device 22 is used for passing light with different wavelengths, and comprises a plurality of imaging filters 221, wherein one imaging filter 221 is arranged on the light path between the detection chamber 33 and the detection device 21, so that light with one wavelength enters the detection device 21; by switching the imaging optical filter in the optical path, the light with different wavelengths respectively enters the detection device. For example, the filter switching device 22 may be a filter wheel including a wheel and a plurality of imaging filters circumferentially arrayed on the wheel, wherein one imaging filter is located on the optical path between the detection chamber 33 and the detection device. The imaging filter is used for filtering the fluorescence of the particles f, for example, only one fluorescence is passed, thereby detecting one fluorescence signal; the wheel is rotated to switch the imaging filter 221, and another fluorescence signal can be detected.

The controller 1 turns on the light source to irradiate the particle f, so that the fluorescent substance coupled to the inside or the surface of the particle f is excited, thereby emitting fluorescence. The controller 1 activates the detection device 21 to detect the fluorescence of the particles f in the detection chamber 33 when the light source is turned on or after the light source irradiates the particles f in the detection chamber. The detection may be performed by photographing the particles f in the detection chamber 33 to obtain a picture of the particles f. Due to the function of the imaging filter 221, light emitted by the light source is filtered, and the quality of the picture is high. After obtaining the picture of the particle f, the controller 1 may perform image processing or the like on the picture to obtain the fluorescence intensity of the particle f, thereby determining the type of the particle f and the amount of the analyte bound to the particle f. From the results of the detection of all the particles f on the photograph, the content or concentration of one or more analytes in the sample, etc. can be obtained.

The detection means 21 may comprise: at least one of a single point photosensitive detector (photodetector), a line array of photosensitive detectors, and an area array of photosensitive detectors. The area array of photosensitive detectors may preferably employ a camera. In addition, the detection device 21 may include one or more photosensitive detectors.

After the cleaning step S4.1 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. After the sample is sucked, the present embodiment is described by taking the detection completed as an example, the controller 1 further controls the driving device to make the sampling needle a located at the suction level, the sampling needle a is communicated with the first fluid power source 31 through the detection chamber 33, and the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the cleaning liquid on the suction level to clean the sampling needle a. The controller 1 controls the driving device to enable the sampling needle a to be positioned at the liquid suction level, and the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a. In this step, before or after cleaning the sampling needle a, the controller 1 further controls the first gating device 34 to switch to communicate the inlet of the detection chamber 33 with the waste liquid recovery device 38, controls the first fluid power source 31 to supply power, and discharges the cleaning liquid from the first fluid power source 31 to at least one section of the first pipe g1, the sample in the detection chamber 33, and the liquid from the detection chamber 33 to the first gating device 34 to the waste liquid recovery device 38 through the second pipe g2 to discharge waste liquid.

Since there is the cleaning fluid drawn in step S1.1 from the first fluid power source 31 to the first conduit g1 (which may be in the first fluid power source 31 and/or in the first conduit g 1), the fluid path through which the sample passes can be cleaned by the cleaning fluid flushing the detection chamber 33 before or after the sample needle a is subjected to the suction flushing as the section of cleaning fluid is pumped out. It can be seen that, in the liquid path subsystem shown in fig. 6, the sample loading process not only can position the sample in one step, but also can clean the particles in the detection chamber 33, and even the cleaning of the sampling needle a is completed by sucking the cleaning liquid in the sample loading process; while the sample is discharged, the cleaning of the detection chamber 33 can be completed, and the sample discharging and cleaning of the detection chamber 33 are performed in one step. It can be seen that the liquid path subsystem shown in fig. 6 is simple and efficient in the processes of loading, discharging, cleaning the sampling needle a and the detection chamber 33, and does not need a sample loop for temporarily storing a sample.

In the second embodiment (the embodiment of the liquid path subsystem shown in fig. 7), in this embodiment, on the basis of the liquid path subsystem shown in fig. 5, a second gating device 32 is added to the liquid path subsystem. The first fluid power source 31 is connected to the outlet of the detection chamber 33 via a first conduit g1 and a second gate 32 in that order. The second gate 32 has a drain port for draining liquid towards the waste liquid recovery device 38. The drain port may directly drain the waste liquid recovery device 38, or may communicate with the waste liquid recovery device 38 via a third pipe g3 to drain the waste liquid recovery device 38 via a third pipe g 3. The third pipe g3 may be any type of pipe as long as it can drain liquid, and the present invention is not limited thereto.

In this embodiment, the second gating device 32 is used for gating the first fluid power source 31, the detection chamber 33 and the third pipeline g3, and specifically, the second gating device 32 is used for switching the outlet of the detection chamber 33 to communicate with the first fluid power source 31 and switching the drain to communicate with the first fluid power source 31. The second gating device 32 may be composed of a valve, or a valve and a pipe; of course, the second gating device 32 may have other structures, such as a plurality of pipes, valves, etc., as long as the first fluid power source 31 is switched to provide power for drawing sample and liquid, and the first fluid power source 31 is switched to discharge waste liquid to the waste liquid recovery device 38.

In this embodiment, the controller 1 controls the optical path subsystem 2, the liquid path subsystem 3, the particle confinement device 4 and the driving subsystem 5 to detect one or more particles, and includes the following steps:

the preprocessing step S1.2, the controller 1 controls the second gating device 32 to communicate the first fluid power source 31 with the outlet of the detection chamber 33, and controls the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning liquid from the cleaning liquid container 37 until the whole liquid path is filled with the cleaning liquid. For example, the entire fluid path shown in fig. 7 is sealed, the first fluid power source 31 includes a pump, the pump is in a pumping operation, the cleaning fluid is sucked into the fluid path, the pump continues to operate to fill the entire fluid path with the cleaning fluid, and the pump further has a continuous pumping capability for subsequent pumping of the sample. Also, it is not necessary to fill the entire liquid path with cleaning solution prior to aspiration. The controller 1 controls the second gating device 32 to communicate the first fluid power source 31 with the outlet of the detection chamber 33, and controls the first fluid power source 31 to provide power to enable the sampling needle a to suck the cleaning liquid from the cleaning liquid container 37, wherein the sucked cleaning liquid is used for subsequently cleaning the pipeline between the first fluid power source 31 and the second gating device 32, and the sucked cleaning liquid is at least required to be in an amount required for cleaning the pipeline between the first fluid power source 31 and the second gating device 32 after particle detection.

And a sample sucking step S2.2, wherein the controller 1 controls the driving device to enable the sampling needle a to be positioned at a sample sucking position. In this manner, the first fluid power source 31, the second gating device 32, the detection chamber 33, the sampling needle a, and the sample container 36 are in communication in sequence. And in turn controls the first fluid power source 31 to provide power to cause the sampling needle a to draw a sample from a sample container 36 placed at the draw position b and deliver the drawn sample to the detection chamber 33. The amount of sample aspirated depends on the needs of the assay. The utility model discloses a sample directly gets into detection room 33 from sample container 36, need not to keep in the sample loop of prior art temporarily, therefore not only the simple efficient of process, the particle of pipeline loss is few moreover.

In this step, after the sample is sucked, the sucked sample is transferred to the detection chamber 33, and the specific process is basically the same as that in the first embodiment, and therefore, the detailed description is omitted.

The detection step S3.2, the controller 1 controls the detection device to detect the optical signal of the particle f in the detection chamber. Similarly, this step is the same as step S3.1 in the previous embodiment, and therefore is not described again.

After the cleaning step S4.2 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. After the detection is completed, the controller 1 further controls the driving device to make the sampling needle a located at the suction level, the sampling needle is communicated with the first fluid power source 31 through the detection chamber 33 and the second gating device 32, of course, the control of the driving device to make the sampling needle a located at the suction level can also be performed before the detection is completed, as long as the control is performed after the sample suction, when the subsequent cleaning liquid is sucked to clean the needle, the sampling needle a is located at the suction level, the sampling needle a is communicated with the first fluid power source 31 through the detection chamber 33, then the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the cleaning liquid on the suction level to clean the sampling needle a and the detection chamber 33, after the sampling needle a and the detection chamber 33 are cleaned, the first fluid power source 31 sucks the sample, the cleaning liquid for cleaning the sampling needle and the detection chamber 33 into the first fluid power source 31 and/or the first pipeline g1, and then the controller 1 controls the second gating device 32 to perform switching, the first fluid power source 31 is caused to communicate with the waste liquid recovery device 38, the first fluid power source 31 is controlled to supply power, and waste liquid (a sample, a cleaning liquid for cleaning the sampling needle a and the detection chamber 33, etc.) from the first fluid power source 31 to the first pipe g1 is discharged to the waste liquid recovery device 38 through the third pipe g 3.

Embodiment three (the embodiment of the liquid path subsystem shown in fig. 8), this embodiment is equivalent to that in fig. 5, the first fluid power source 31 is changed from bidirectional to unidirectional, and the first fluid power source 31 has an inlet and a liquid discharge port for discharging liquid toward the waste liquid recovery device 38. In this embodiment, the direction of the flow of the liquid is unidirectional, as indicated by the arrows, for example, the first fluid power source 31 may employ a unidirectional pump to achieve unidirectional flow of the liquid. The inlet of the first fluid power source 31 may be connected directly to the outlet of the sensing chamber 33 or may be connected to the outlet of the sensing chamber 33 via a first conduit g 1. The inlet of the sensing chamber 33 may be directly connected to the sampling needle a, or may be connected to the sampling needle a through a second tube g 2.

and a preprocessing step S1.3, controlling the driving device to drive by the controller 1 to enable the sampling needle a to be at a liquid suction level, and controlling the first fluid power source 31 to provide power to enable the sampling needle a to suck the cleaning liquid from the cleaning liquid container 37 until the whole liquid path is filled with the cleaning liquid. It is not necessary to fill the entire liquid path with the wash liquid prior to aspiration. Regardless of whether the entire fluid path is filled with cleaning fluid, the controller 1 may also control the first fluid power source 31 to provide power to cause the sampling needle a to draw cleaning fluid from the cleaning fluid reservoir 37, although such action is not required.

And a sample sucking step S2.3, wherein the controller 1 controls the driving device to enable the sampling needle a to be positioned at a sample sucking position. In this way, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected in this order. And in turn controls the first fluid power source 31 to provide power to cause the sampling needle a to draw a sample from a sample container 36 placed at the draw position b and deliver the drawn sample to the detection chamber 33. The amount of sample aspirated depends on the needs of the assay. The utility model discloses a sample directly gets into detection room 33 from sample container 36, need not to keep in the sample loop of prior art temporarily, therefore not only the simple efficient of process, the particle of pipeline loss is few moreover.

In the detection step S3.3, the controller 1 controls the detection device to detect the optical signal of the particle f in the detection chamber. Similarly, this step is the same as step S3.2 in the previous embodiment, and therefore is not described again.

After the cleaning step S4.3 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. After the detection is finished, if the sampling needle a is not positioned at the suction liquid level and the sampling needle is not communicated with the first fluid power source 31, the controller 1 further controls the driving device to make the sampling needle a positioned at the suction liquid level, the sampling needle is communicated with the first fluid power source 31 through the detection chamber 33, and then the first fluid power source 31 is controlled to provide power to make the sampling needle a suck cleaning liquid on the suction liquid level to clean the sampling needle a and the detection chamber 33; after the detection is finished, if the sampling needle a is located at the suction liquid level (for example, the sampling needle a is located at the suction liquid level by the driving device before), and the sampling needle is communicated with the first fluid power source 31, directly controlling the first fluid power source 31 to provide power to enable the sampling needle a to suck cleaning liquid on the suction liquid level to clean the sampling needle a and the detection chamber 33; the sample, the cleaning liquid for cleaning the sampling needle and the detection chamber 33 are then discharged to the waste liquid recovery device 38 through the liquid discharge port.

In the fourth embodiment (the embodiment of the fluid path subsystem shown in fig. 9), it is equivalent to adding a second gating device 32 between the first fluid power source 31 and the detection chamber 33 on the basis of the fluid path shown in fig. 6. In this embodiment, the first fluid power source 31 is connected to the outlet of the detection chamber 33 via a first conduit g1 and a second gate 32 in sequence, the second gate 32 also being in communication with the cleaning solution container 37' via a third conduit g 3. The second gate 32 is adapted to communicate at least one of the third conduit g3 and the sensing chamber 33 with the first source 31 of fluid power, either at the same time as only one of the sensing chamber 33 and the third conduit g3 is able to communicate with the first source 31 of fluid power, or at the same time as both the sensing chamber 33 and the third conduit g3 are in communication with the first source 31 of fluid power. The second gate 32 may be composed of a valve, as shown in fig. 15, and the second gate 32 includes a second valve

K including joints

1, 2 and 3 for inflow and outflow of the liquid. A first fluid power source 31, for example a pump, is connected via a first conduit g1 to a connector 1 of a second valve K, a connector 2 of the second valve K being connected to one end of a third conduit g3, the other end of the third conduit g3 being used to draw liquid from the cleaning liquid container 37'. The connector 3 of the second valve K is connected to the outlet of the detection chamber 33. Controller 1 controls connection 1 of second valve K to communicate with connection 2 and connection 3 to be closed, and third conduit g3 to communicate with first source of fluid power 31. The controller 1 controls the connection 1 of the second valve K to communicate with the connection 3, and the connection 2 is closed, and the detection chamber 33 communicates with the first fluid power source 31. The second gating device 32 may also be composed of valves and pipes, as shown in fig. 16, and the second gating device 32 includes a third valve L, a fourth valve (not shown), and pipes. A first fluid power source 31, for example a pump, having two ports for pumping in and out a fluid, one port being connected to the outlet of the detection chamber 33 via a first conduit, the other port being connected to the outlet of a third valve L via a tube, the inlet of the third valve L being connected to a third conduit g3, a fourth valve being arranged in the first conduit or in the fluid path between the detection chamber 33 and the sampling needle a; thus, the third valve L is turned off, and the fourth valve is turned on, so that the pump 31 can suck liquid through the liquid path formed by the detection chamber 33 and the sampling needle a; the fourth valve is turned off, and the third valve L is turned on, so that the pump 31 can suck liquid from the cleaning liquid container 37'; of course, the second gating device 32 may have other configurations, such as a plurality of pipes, valves, etc., as long as the first fluid power source 31 can suck the cleaning solution from the cleaning solution container 37' by switching, and the first fluid power source 31 can provide power to suck and discharge the cleaning solution.

in the preprocessing step S1.4, the controller 1 controls the second gating device 32 to communicate the first fluid power source 31 with the detection chamber 33, and then the preprocessing may be performed in the preprocessing step S1.1 of the first embodiment, or of course, the second gating device 32 may be controlled to communicate the first fluid power source 31 with the cleaning liquid container 37', and the controller 1 may control the first fluid power source 31 to suck the cleaning liquid from the cleaning liquid container 37, and further, the liquid path may be filled with the cleaning liquid.

The sample sucking step S2.4 may be substantially the same as the sample sucking step S2.2 in the second embodiment, and is not described herein again.

The detection step S3.4, the controller 1 controls the detection device to detect the optical signal of the particle f in the detection chamber. Similarly, this step is the same as step S3.1 in the first embodiment, and therefore is not described again.

After the cleaning step S4.4 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. In this embodiment, after the detection is completed, the controller 1 may control the second gating device 32, the first gating device 34 and the driving device to communicate the first fluid power source 31 with the cleaning solution container 37, and further control the first fluid power source 31 to suck the cleaning solution from the cleaning solution container 37 to clean the sampling needle a. After cleaning the sampling needle a, the controller 1 may control the second gating device 32 to communicate the first fluid power source 31 with the cleaning solution container 37 ', and further control the first fluid power source 31 to suck the cleaning solution from the cleaning solution container 37' to clean the detection chamber 33, and discharge the cleaned waste solution to the waste solution recovery device 38.

The cleaning solution container 37 'communicated with the third pipe g3 and the cleaning solution container 37 at the liquid suction position d may be different cleaning solution containers, for example, two separate containers, and of course, the cleaning solution container 37' communicated with the third pipe g3 and the cleaning solution container 37 at the liquid suction position may also be the same cleaning solution container, for example, the cleaning solution container is communicated with the first gating device 34 through the sampling needle a and communicated with the second gating device 32 through the third pipe g 3; it is thus clear that the utility model discloses do not restrict to the washing liquid container.

Fifth embodiment (the embodiment of the fluid path subsystem shown in fig. 10), this embodiment is equivalent to adding the second gating device 32, the first gating device 34, and the second fluid power source 39 to the fluid path shown in fig. 5. The first fluid power source 31 is connected to the outlet of the detection chamber 33 by a second gating device 32. The first gate 34 of this embodiment has a first outlet 1, an inlet 2 and a second outlet 3. The first outlet 1 of the first gating device 34 communicates with the inlet of the detection chamber 33, for example, the first outlet 1 of the first gating device 34 is directly connected to the inlet of the detection chamber 33 or connected through a pipe; the inlet 2 of the first gating device 34 is connected to the sampling needle a, the second outlet 3 of the first gating device 34 is in communication with the second fluid power source 39, for example, the second outlet 3 of the first gating device 34 is connected to the second fluid power source 39 directly or through a conduit.

The first gating device 34 is used for gating the detection chamber 33, the sampling needle a and the second fluid power source 39, and there may be various ways, for example, a first outlet 1 of the first gating device is communicated with an inlet 2 thereof, and the first gating device is used for controlling the communication and disconnection between the first outlet 1 and the second outlet 3 thereof (for example, a valve is arranged between the first outlet 1 and the second outlet 3); one is that the first outlet 1 of the first gating means communicates with the second outlet 3 thereof, and the first gating means is used for controlling the communication and disconnection between the inlet 2 thereof and the first outlet 1 thereof (for example, by providing a valve between the inlet 2 and the first outlet 1). The first gating means is at least used for switching the inlet 2 to communicate with the first outlet 1 and the first outlet 1 to communicate with the second outlet 3, and in an alternative embodiment, can also be used for switching the inlet 2 to communicate with the second outlet 3. The present embodiment takes the last mode of the first gating device as an example.

Second fluid power source 39 is used to provide power for the flow of liquid in the fluid path, and in particular, may be used to provide power to clean sensing chamber 33. Second fluid power source 39 is a device capable of driving a flow of liquid in a conduit, such as various types of pumps or combinations of multiple pumps connected by tubing, valves, or the like. The first and second

fluid power sources

31, 39 may be two separate fluid power devices, such as pumps, for example, although the first and second

fluid power sources

31, 39 may be the same fluid power device, such as pumps, for example, for different uses through different connections of valves, pipes, etc. Of course, the first fluid power source 31 and the second fluid power source 39 may also share some pumps, with the shared pump serving a different purpose when in use. Both are the same fluid power device, for example, the fluid power device has a port 1 for pumping in and out liquid, and a port 2 for pumping in and out liquid, the port 1 communicating with the second gate 32, the port 2 communicating with the first gate 34.

The second gate 32 has a drain port for draining liquid towards the waste liquid recovery device 38. The second gating means 32 gates all of the first fluid power source 31, the detection chamber 33, and the drain port, and this embodiment will be described by taking as an example the gating of all of the first fluid power source 31, the detection chamber 33, and the third duct g 3. For example, the second gating means 32 may switch the third channel g3 to communicate with the outlet of the detection chamber 33 and switch the outlet of the detection chamber 33 to communicate with the first fluid power source 31, and the second fluid power source 39 supplies power to wash the detection chamber 33 with the washing liquid and discharge the waste liquid after washing the detection chamber 33 to the waste liquid recovery device 38 communicated with the third channel g 3. For another example, the second gating device 32 switches the third conduit g3 to communicate with the first fluid power source 31, and switches the outlet of the detection chamber 33 to communicate with the first fluid power source 31; this gating corresponds to the second fluid power source 39 providing power to wash the detection chamber 33 with the cleaning solution and discharge the waste fluid from the washed detection chamber 33 to the first fluid power source 31 and/or the second gating device 32, and then the second gating device 32 switches to allow the first fluid power source 31 to provide power to discharge the waste fluid through the second gating device 32 to the waste fluid recovery device 38 connected to the third conduit g 3.

In this embodiment, the first fluid power source 31 is connected to the outlet of the detection chamber 33 via the first conduit g1 and the second gate 32 in this order, and the second gate 32 is further connected to the waste liquid recovery device 38 via the third conduit g 3.

in the preprocessing step S1.5, the controller 1 may control the second gating device 32 to communicate the first fluid power source 31 with the detection chamber 33, the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, the controller 1 controls the driving device to position the sampling needle a at the suction level d, and the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the cleaning solution from the cleaning solution container 37 until the whole liquid path is filled with the cleaning solution. It is not necessary to fill the entire liquid path with the wash liquid prior to aspiration.

The controller 1 may also control the first gating device 34 to communicate the second fluid power source 39 with the sampling needle a, control the driving device to make the sampling needle a at a suction level, control the second fluid power source 39 to suck the cleaning liquid from the cleaning liquid container 37, and make the second fluid power source 39 and/or a pipeline between the first gating device 34 and the second fluid power source 39 have the cleaning liquid therein for subsequent cleaning; this process may be performed in this step, or after cleaning the sampling needle in step S2.5, S3.5 or S4.5.

And a sample sucking step S2.5, wherein the controller 1 controls the second gating device 32 to enable the first fluid power source 31 to be communicated with the detection chamber 33, controls the first gating device 34 to enable the inlet of the detection chamber 33 to be communicated with the sampling needle a, and controls the driving device to enable the sampling needle a to be positioned at a sample sucking position. And further controls the first fluid power source 31 to provide power to enable the sampling needle a to suck a sample from the sample container 36 placed on the sample sucking position b, and controls the first fluid power source 31 or the second fluid power source 39 to convey the sample sucked into the liquid path to the detection chamber 33, wherein in the embodiment, the first fluid power source 31 conveys the sucked sample to the detection chamber 33.

In this step, after the sample is sucked, four ways may be specifically used to transport the sucked sample to the detection chamber 33, and the specific process is the same as that in the first embodiment, which is not described herein again.

The detection step S3.5 is the same as the detection step of the first embodiment, and therefore is not described in detail.

After the cleaning step S4.5 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. In this embodiment, after the detection is completed, the controller 1 further controls the driving device to make the sampling needle a located at the suction level, controls the second gating device 32 to make the first fluid power source 31 communicate with the outlet of the detection chamber 33, and controls the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning solution at the suction level to clean the sampling needle a. In this step, before or after the cleaning of the sampling needle a, preferably after the cleaning of the sampling needle a, the controller 1 further controls the second gating device 32 to switch so that the outlet of the detection chamber 33 communicates with the waste liquid recovery device 38, controls the first gating device 34 to switch so that the inlet of the detection chamber 33 communicates with the second fluid power source 39, further controls the second fluid power source 39 to supply power, and discharges the cleaning liquid in the second fluid power source 39 and/or the cleaning liquid between the second fluid power source 39 and the first gating device 34, the sample of the detection chamber 33, and the like to the waste liquid recovery device 38, and completes the cleaning of the detection chamber 33 when the cleaning liquid passes through the detection chamber 33.

In the above embodiment, both the sample aspirating and needle washing are powered by the first fluid power source 31, and in the subsequent embodiment, the sample aspirating is powered by the first fluid power source 31 and the needle washing is powered by the second fluid power source 39. As shown in fig. 11, the liquid path subsystem at least includes: a sampling needle a, a detection chamber 33, a first fluid power source 31, a first gating device 34 for selective communication with a second fluid power source 39.

The first fluid power source 31 communicates with an outlet of the detection chamber 33. The inlet of the detection chamber 33 and the second source of fluid power 39 are both connected to the sampling needle a by a first gating device 34. Wherein the second fluid power source 39 is in communication with the first gating device 34, it may be directly connected to the first gating device 34, or it may be connected to the first gating device 34 via a second conduit g 2. The first gating device 34 is used for gating, and there may be various ways, for example, one is that the first outlet 1 of the first gating device is communicated with the inlet 2 thereof, that is, the sampling needle and the inlet of the detection chamber can be communicated through the first gating device 34, and the first gating device is used for controlling the communication and disconnection between the inlet 2 thereof and the second outlet 3 thereof (for example, a valve is arranged between the inlet 2 and the second outlet 3); one is that the inlet 2 of the first gating means is in communication with the second outlet 3 thereof, i.e. the sampling needle and the second source of fluid power may be brought into communication by the first gating means which is arranged to control the connection and disconnection between the inlet 2 thereof and the first outlet 1 thereof (e.g. by providing a valve between the inlet 2 and the first outlet 1). The first gating means is at least used for switching the inlet 2 to communicate with the first outlet 1 and the first outlet 1 to communicate with the second outlet 3, and in an alternative embodiment, can also be used for switching the inlet 2 to communicate with the second outlet 3. The present embodiment takes the last mode of the first gating device as an example.

The sampling needle a is used to draw a sample in dependence upon the power provided by the first fluid power source 31 and to draw cleaning fluid in dependence upon the power provided by the first fluid power source 31 or the second fluid power source 39.

The controller 1 is configured to control the optical path subsystem 2, the liquid path subsystem 3, and the driving subsystem 5 to implement detection of one or more particles, for example, a user manually places the sample container 36 under the sampling needle a, and places the sampling needle a under the liquid level, and then makes the inlet of the detection chamber 33 communicate with the sampling needle a through the first gating device 34, and of course, the inlet of the detection chamber 33 may be first communicated with the sampling needle a, and then places the sample container 36 under the sampling needle a, or both may be performed simultaneously, and the sequence of the two is not limited; the first fluid power source 31 is controlled to provide power to enable the sampling needle a to suck the sample in the sample container 36, the first fluid power source 31 or the second fluid power source 39 is controlled to convey the sample sucked into the liquid path to the detection chamber 33, for example, the first fluid power source 31 is controlled to provide power to enable the sampling needle a to suck the sample at the sample sucking position b and convey the sucked sample to the detection chamber 33, for example, the first fluid power source 31 is controlled to provide power to enable the sampling needle a to suck the sample at the sample sucking position b, after the sample sucking is completed, the second fluid power source 39 is communicated with the inlet of the detection chamber 33 through the first gating device 34, and the second fluid power source 39 is controlled to convey the sample sucked into the liquid path to the detection chamber 33 (the first fluid power device 31 can also be simultaneously controlled to assist in conveying the sample sucked into the liquid path to the detection chamber 33). After the sample is sucked, the user manually places the cleaning liquid container 37 below the sampling needle a and enables the sampling needle a to be below the liquid level, after the sample is sucked, the sampling needle a is communicated with the second fluid power source 39 through the first gating device 34, similarly, the cleaning liquid container 37 is placed below the sampling needle a and is communicated with the sampling needle a and the second fluid power source 39, the sequence of the sampling needle a and the second fluid power source 39 is not limited, and the second fluid power source 39 is controlled to provide power to enable the sampling needle a to suck the cleaning liquid to clean the sampling needle a. Because the sampling needle a is cleaned by adopting the second fluid power source 39, the cleaning time is flexible, the sampling needle can be cleaned after the sample suction is finished, and the efficiency is high. Wherein the sample in the sample container 36 is sucked into the liquid path and the suction is considered to be completed without affecting the detection of the final sample when the second fluid power source 39 sucks and cleans the sampling needle a. For example, the sample in the sample container 36 is sucked into the liquid path, and the sample with the required amount for detection is discharged from the outlet 1 of the first gating device 34, and then the second fluid power source 39 sucks and cleans the sampling needle a without affecting the detection of the final sample. Of course, the sample that does not satisfy the amount required for detection can be regarded as the sample sucking is completed through the outlet 1 of the first gating device 34, as long as the detection of the final sample is not affected when the second fluid power source 39 sucks and cleans the sampling needle a (i.e. after the sample sucking is completed, the sample that satisfies the amount required for detection always finally exits through the outlet 1 of the first gating device 34 by the driving of the subsequent fluid path). The working time of the fluid power source is a control parameter, so that the sample suction can be finished if the sample suction preset time is considered; the preset time is set according to the requirement, the amount of the sample required for detection can be ensured, and the detection of the final sample cannot be influenced when the second fluid power source 39 sucks the liquid and cleans the sampling needle a.

The above process is a manual sample loading process for a user, and of course, the sample loading process may also be automatic, which is described in detail below. As shown in fig. 11, the detection system further includes: a sample sucking position b, a liquid sucking position d and a driving device.

And the driving device is used for driving the sampling needle a and/or the sample sucking position b to move so that the sampling needle a is positioned at the sample sucking position b when sucking samples, and is used for driving the sampling needle a and/or the liquid sucking position d to move so that the sampling needle a is positioned at the liquid sucking position d when sucking the cleaning liquid. Wherein the sample sucking position b and the liquid sucking position d can be different positions, for example, the driving subsystem 5 further comprises a tray, one position of the tray is used for placing the sample container 36, the sample sucking position b is used for placing the cleaning liquid container 37, the other position of the tray is used for placing the cleaning liquid container d, and the driving device moves the sampling needle a and/or the tray so that the sampling needle a is positioned at the sample sucking position during sample sucking and the sampling needle a is positioned at the liquid sucking position during liquid sucking. Of course, the sample suction position b and the liquid suction position d may be the same position, for example, the driving subsystem 5 further includes a tray for placing the sample container 36 and the cleaning solution container 37 at a position, the sample suction position b is the position where the sample container 36 is placed, and the liquid suction position d is the position where the cleaning solution container 37 is placed.

The controller 1 is used for controlling the optical path subsystem 2, the liquid path subsystem 3 and the driving subsystem 5 to realize detection of one or more particles, for example, the controller 1 controls the first gating device 34 to gate, the first fluid power source 31 to operate, the second fluid power source 39 to operate and the driving device to drive, when the controller 1 controls the driving device to enable the sampling needle a to be located at the sample sucking position b, the sampling needle a is communicated with the inlet of the detection chamber 33 through the first gating device 31, similarly, the driving device enables the sampling needle a to be located at the sample sucking position b and the first gating device 31 is communicated with the inlet of the detection chamber 33, and the two are not limited in sequence; the first fluid power source 31 is controlled to provide power to enable the sampling needle a to suck a sample at the sample sucking position b, and the first fluid power source 31 or the second fluid power source 39 is controlled to convey the sample sucked into the liquid path to the detection chamber 33. After the sample suction is finished, the sampling needle a is communicated with the second fluid power source 39 through the first gating device 34, the controller 1 controls the second fluid power source 39 to provide power, so that the sampling needle a sucks cleaning liquid at the liquid suction position d to clean the sampling needle, and before the sampling needle a sucks the cleaning liquid at the liquid suction position d, the controller 1 also controls the driving device to make the sampling needle a be located at the liquid suction position d, so that the cleaning liquid is sucked from the liquid suction position d to be cleaned when the sampling needle a is cleaned. Controlling the driving device to enable the sampling needle a to be positioned at the liquid suction level d after the sample is sucked, so as to ensure that the sampling needle a is positioned at the liquid suction level d when the sampling needle is cleaned; for example, the controller 1 controls the driving device to position the sampling needle a at the suction level d immediately after the sample suction, or the controller 1 controls the driving device to position the sampling needle a at the suction level d after the sample suction is completed. After the sample suction, the present embodiment will be described by taking the sample suction completed as an example, the controller 1 controls the driving device to make the sampling needle a located at the suction level d, and makes the sampling needle a and the second fluid power source 39 communicate through the first gating device 34, and similarly, the driving device makes the sampling needle a located at the suction level d and makes the sampling needle a and the second fluid power source 39 communicate through the first gating device 34, and the sequence of the two is not limited; the second fluid power source 39 is controlled to provide power to make the sampling needle a suck the cleaning liquid at the liquid suction position d to clean the sampling needle. Further, sampling needle a absorbs the washing liquid in order to wash the sampling needle on imbibition position d, and sampling needle a specifically washs the sampling needle with the mode of absorbing the washing liquid on imbibition position d, promptly, after sampling needle a absorbs the sample, has the sample to remain in its pipe wall, and when follow-up sampling needle a absorbs the washing liquid, the washing liquid will be taken away remaining sample, has accomplished the washing. Because the sample sucking and the cleaning liquid sucking are both the same sampling needle a, the cleaning liquid sucking is just to clean the sampling needle a, and the cleaning process is simple and efficient. Because the second fluid power source 39 is used to provide power to clean the sampling needle a, the cleaning of the sampling needle a and the detection of the sample in the detection chamber 33 can be performed simultaneously, and the efficiency is high.

Further, in the schematic diagram shown in fig. 11, the first fluid power source 31 communicates with the outlet of the detection chamber 33, and may be directly connected to the outlet of the detection chamber 33 or connected to the outlet of the detection chamber 33 through the first duct g 1.

FIG. 11 is a schematic block diagram of a fluid path sub-system, and the specific fluid path sub-system can be in various forms, as shown in FIGS. 10 and 12, the direction of the arrows in FIGS. 10 and 12 indicate the flow direction of the fluid, and the single arrow indicates that the fluid can only flow in one direction; the double arrows indicate that flow is possible in both directions, i.e. one time may flow in one direction and another time may flow in the other direction. The two fluid path subsystems shown in fig. 10 and 12 are described as two embodiments.

Sixth embodiment (an embodiment of the liquid path subsystem shown in fig. 12), in this embodiment, in addition to fig. 11, the first gating device 34 has a liquid discharge port for discharging liquid toward the waste liquid recovery device 38.

In this embodiment, the first gate 34 has a first outlet 1, an inlet 2, a second outlet 3, and a drain 4. The first gating device 34 gates the detection chamber 33, the sampling needle a, the second fluid power source 39 and the liquid discharge port 3, specifically, for switching to communicate the first outlet 1 with the inlet 2; switching to communicate the first outlet 1 with the liquid discharge port 4; the inlet 2 is communicated with the second outlet 3 through switching; in an alternative embodiment, the second outlet 3 may also be switched to communicate with the liquid discharge port 4. The first outlet 1 of the first gating device 34 is connected to the inlet of the detection chamber 33, the inlet 2 of the first gating device 34 is connected to the sampling needle a, the second outlet 3 of the first gating device 34 is connected to the second fluid power source 39, and the drain 4 of the first gating device 34 is used for draining liquid to the waste liquid recovery device 38.

in the preprocessing step S1.6, the controller 1 may control the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, control the driving device to make the sampling needle a located at the suction level d, and control the first fluid power source 31 to provide power to make the sampling needle a suck the cleaning solution from the cleaning solution container 37 until the whole solution path is filled with the cleaning solution. It is not necessary to fill the entire liquid path with the wash liquid prior to aspiration.

The controller 1 may also control the first gating device 34 to communicate the second fluid power source 39 with the sampling needle a, control the drive means to bring the sampling needle a to a suction level, and control the second fluid power source 39 to draw cleaning fluid from the cleaning fluid reservoir 37, with the second fluid power source 39 and/or the conduit between the first gating device 34 and the second fluid power source 39 having cleaning fluid therein for subsequent cleaning. In this embodiment, the cleaning of the detection chamber 33 is performed by the first fluid power source 31, for example, the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, controls the driving device to make the sampling needle a at the suction level, and controls the first fluid power source 31 to suck the cleaning liquid from the cleaning liquid container 37, so that the first fluid power source 31 and/or the pipeline between the first fluid power source 31 and the detection chamber 33 has the cleaning liquid therein to be used for subsequently cleaning the detection chamber 33.

And a sample sucking step S2.6, wherein the controller 1 controls the driving device to enable the sampling needle a to be positioned at a sample sucking position, and the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, and the two actions are not in sequence or can be carried out simultaneously. In this way, the first fluid power source 31, the detection chamber 33, the sampling needle a, and the sample container 36 are connected in this order. And in turn controls the first fluid power source 31 to provide power to cause the sampling needle a to draw a sample from a sample container 36 placed at the sample drawing position b, which in this embodiment is delivered to the detection chamber 33 by the first fluid power source 31. The amount of sample aspirated depends on the needs of the assay. The utility model discloses a sample directly gets into detection room 33 from sample container 36, need not to keep in the sample loop of prior art temporarily, therefore not only the simple efficient of process, the particle of pipeline loss is few moreover.

The detection step S3.6 is the same as the detection step of the first embodiment, and is not described herein again.

After the cleaning step S4.6 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. After the detection is completed, the controller 1 further controls the first gating device 34 to switch, so that the inlet of the detection chamber 33 is communicated with the waste liquid recovery device 38, controls the first fluid power source 31 to provide power to enable the cleaning liquid in the first fluid power source 31 and/or the cleaning liquid between the first fluid power source 31 and the outlet of the detection chamber 33 to pass through the detection chamber 33 to clean the detection chamber 33, and finally discharges waste liquid such as a sample to the waste liquid recovery device 38.

The cleaning of the sampling needle a may be performed in the sample aspirating step, the detecting step, or the cleaning step, as long as the cleaning is performed after the sample aspirating is completed, and this embodiment is not limited. Of course, the cleaning liquid after cleaning the sampling needle a needs to be discharged to the waste liquid recovery device 38, and this process may be performed by the first fluid power source or the second fluid power source.

Seventh embodiment, as shown in fig. 10, the liquid path connection relationship of the present embodiment is equivalent to that in fig. 11, a second gate device 32 is provided between the first fluid power source 31 and the detection chamber 33.

The second gate 32 has a drain port for draining liquid towards the waste liquid recovery device 38. The first fluid power source 31 is connected to the outlet of the detection chamber 33 by a second gating device 32. The second gating means 32 gates all of the first fluid power source 31, the detection chamber 33, and the drain port, and this embodiment will be described by taking as an example the gating of all of the first fluid power source 31, the detection chamber 33, and the third duct g 3. For example, the second gating device 32 may be switched to connect the third pipe g3 with the outlet of the detection chamber 33 and to connect the outlet of the detection chamber 33 with the first fluid power source 31, and, optionally, the second gating device 32 may be switched to connect the third pipe g3 with the first fluid power source 31, in such a way that the second fluid power source 39 provides power to enable the cleaning solution to clean the sampling needle a and the detection chamber 33 and discharge the waste solution after cleaning the sampling needle a and the detection chamber 33 to the waste solution recovery device 38 connected with the third pipe g 3. For another example, the second gating device 32 switches the third conduit g3 to communicate with the first fluid power source 31, and switches the outlet of the detection chamber 33 to communicate with the first fluid power source 31; this gating corresponds to the second fluid power source 39 providing power to wash the sampling needle a and the detection chamber 33 with the cleaning solution and discharging the waste liquid after washing the sampling needle a and the detection chamber 33 to the first fluid power source 31 and/or the second gating device 32, and then the second gating device 32 switches to provide power to the first fluid power source 31 to discharge the waste liquid through the second gating device 32 to the waste liquid recovery device 38 connected to the third pipe g 3.

in the preprocessing step S1.7, the controller 1 may control the second gating device 32 to communicate the first fluid power source 31 with the detection chamber 33, the controller 1 controls the first gating device 34 to communicate the inlet of the detection chamber 33 with the sampling needle a, the controller 1 controls the driving device to position the sampling needle a at the suction level d, and the first fluid power source 31 is controlled to provide power to make the sampling needle a suck the cleaning solution from the cleaning solution container 37 until the whole liquid path is filled with the cleaning solution. It is not necessary to fill the entire liquid path with the wash liquid prior to aspiration.

The controller 1 may also control the first gating device 34 to communicate the second fluid power source 39 with the sampling needle a, control the drive means to bring the sampling needle a to a suction level, and control the second fluid power source 39 to draw cleaning fluid from the cleaning fluid reservoir 37, with the second fluid power source 39 and/or the conduit between the first gating device 34 and the second fluid power source 39 having cleaning fluid therein for subsequent cleaning.

And a sample sucking step S2.7, in which the controller 1 controls the gating of the second gating device 32, the gating of the first gating device 34, the operation of the first fluid power source 31 and the driving of the driving device, when the controller 1 controls the driving device to enable the sampling needle a to be positioned at the sample sucking position, the first gating device 34 is controlled to enable the sampling needle a to be communicated with the inlet of the detection chamber 33, the second gating device 32 is controlled to enable the first fluid power source 31 to be communicated with the outlet of the detection chamber 33, so that the first fluid power source 31, the second gating device 32, the detection chamber 33, the first gating device 34, the sampling needle a and the sample container 36 are communicated in sequence, and the first fluid power source 31 is further controlled to provide power to enable the sampling needle a to suck a sample at the sample sucking position b, wherein the sucked sample is conveyed to the detection chamber 33 by the first fluid power source 31. The amount of sample aspirated depends on the needs of the assay. The utility model discloses a sample directly gets into detection room 33 from sample container 36, need not to keep in the sample loop of prior art temporarily, therefore not only the simple efficient of process, the particle of pipeline loss is few moreover.

In the detection step S3.7, the controller 1 controls the detection device to detect the optical signal of the particle f in the detection chamber. Similarly, this step is the same as step S3.1 in the first embodiment, and therefore is not described again.

After the cleaning step S4.7 and the detection, the controller 1 turns off the particle confinement device 4 or controls the particle confinement device 4 to be away from the detection chamber 33, so that the particles in the detection chamber 33 are not confined by the particle confinement device 4, thereby facilitating the discharge of the particles. For the cleaning of the sampling needle a, after the sample suction is completed, the controller 1 controls the driving device to make the sampling needle a be located at the suction level d, controls the first gating device 34 to make the sampling needle a communicate with the second fluid power source 39, and controls the second fluid power source 39 to provide power to make the sampling needle a suck the cleaning liquid at the suction level d to clean the sampling needle. In this embodiment, after the detection is completed, the controller 1 controls the driving device to make the sampling needle a located at the liquid suction level d, controls the first gating device 34 to switch to make the sampling needle a communicate with the second fluid power source 39, controls the second gating device 32 to switch to make the outlet of the detection chamber 33 communicate with the waste liquid recovery device 38, and controls the second fluid power source 39 to provide power to make the sampling needle a suck the cleaning liquid at the liquid suction level d to clean the sampling needle; and then the first gating device 34 is controlled to switch to enable the inlet of the detection chamber 33 to be communicated with the second fluid power source 39, the second fluid power source 39 is controlled to supply power, the cleaning liquid in the second fluid power source 39 and/or the cleaning liquid between the second fluid power source 39 and the first gating device 34, the detection chamber 33 and the second gating device 32 are discharged to the waste liquid recovery device 38, and the cleaning of the detection chamber 33 and the discharge of waste liquid are completed. Of course, in the fluid path and the sampling needle a of this embodiment, the sample may also be cleaned after the sample suction and before the detection is completed, which is not described in detail.

The solution of the first fluid power source 31 providing power for sucking sample and the second fluid power source 39 providing power for washing needle may also be the solution path shown in fig. 17. The liquid path shown in fig. 17 differs from the liquid path shown in fig. 11 in that: in the fluid path shown in fig. 11, the detection chamber 33, the second fluid power source 39 and the sampling needle a are gated by the first gating means; in the fluid path shown in fig. 17, the detection chamber 33, the second fluid power source 39, and the sampling needle a are connected to each other by a three-way device 341. The three-way device 341 has a first port 1, a second port 2 and a third port 3. The first port 1 of the three-way device 341, the second port of the three-way device 341, and the third port of the three-way device 341 are communicated with each other. The first interface of the three-way device 341 is connected to the inlet of the detection chamber 33, the second interface 2 of the three-way device 341 is connected to the sampling needle a, and the third interface 3 of the three-way device 341 is connected to the second fluid power source 39.

The controller is used for controlling the first fluid power source 31 to provide power to enable the sampling needle a to suck a sample, and controlling the first fluid power source 31 or the second fluid power source 39 to convey the sample sucked into the liquid path to the detection chamber 33; the second fluid power source 39 is controlled to provide power to make the sampling needle a suck cleaning liquid to clean the sampling needle a.

Similarly, for sample loading, the present embodiment may adopt a manual mode or an automatic mode, and the sample loading is manually matched in the manual mode, which is similar to the above embodiments, and therefore, the details are not described herein. The automatic mode further comprises a driving device as described above.

The controller is used for controlling the action of the first fluid power source, the action of the second fluid power source and controlling the driving of the driving device, when the controller controls the driving device to enable the sampling needle to be located at the sample suction position, the first fluid power source is controlled to provide power to enable the sampling needle to suck a sample at the sample suction position, and the first fluid power source or the second fluid power source is controlled to convey the sample sucked into the liquid path to the detection chamber. The controller is also used for controlling the driving device to enable the sampling needle to be positioned at the liquid suction level and controlling the second fluid power source to provide power, so that the sampling needle sucks cleaning liquid on the liquid suction level to clean the sampling needle. For example, after the sample is sucked, the controller controls the driving device to enable the sampling needle to be located at the sucking liquid level, and after the sample is sucked, the second fluid power source is controlled to provide power to enable the sampling needle to suck the cleaning liquid on the sucking liquid level to clean the sampling needle. Taking time control as an example, after the sample is sucked, the controller controls the driving device to enable the sampling needle to be located at the liquid sucking level, and after the sample is sucked for the preset time, the controller controls the second fluid power source to provide power to enable the sampling needle to suck the cleaning liquid on the liquid sucking level to clean the sampling needle. Whether the manual mode or the automatic mode, the process of detecting in this embodiment is basically the same as that in the above embodiment, and therefore, the detailed description thereof is omitted.

In the above embodiment, the sample sucking position b and the liquid sucking position d may be different positions, for example, the driving subsystem 5 further includes a tray on which a position for placing the sample container 36 is at the sample sucking position b, another position for placing the cleaning solution container 37 is at the liquid sucking position d, and the driving device moves the sampling needle a and/or the tray so that the sampling needle a is at the sample sucking position when sucking the sample and the sampling needle a is at the liquid sucking position when sucking the cleaning solution. Of course, the sample suction position b and the liquid suction position d may be the same position, for example, the driving subsystem 5 further includes a tray, a position on the tray is used for placing both the sample container 36 and the waste liquid recovery device 38, the sample suction position b is the position where the sample container 36 is placed, and the liquid suction position d is the position where the cleaning liquid container 37 is placed; for another example, the sample sucking position b and the liquid sucking position d are the same coordinate position, and when a sample needs to be sucked, the sampling needle and/or the sample container moves to the position to realize the collection of the sample, and when a cleaning liquid needs to be sucked, the sampling needle and/or the cleaning liquid container moves to the position to realize the suction of the cleaning liquid. The sample container 36 may be used as a waste liquid recovery device after completion of the sample suction. After the cleaning liquid is sucked, the cleaning liquid container can also be used as a waste liquid recovery device.

To sum up, the utility model discloses can directly carry the detection room with the sample through the sampling needle, can wash the sampling needle at the in-process that the sampling needle absorbs the washing liquid again. It is thus clear that the utility model discloses a to the design of liquid way subsystem, can make the sample transport flow of system obtain simplifying, improve detection efficiency, can realize the washing to the sampling needle again, reduce the sample and remain the interference that causes follow-up detection.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims

1. A detection system for analyzing one or more particles, comprising:

a first fluid power source for powering a flow of liquid in the fluid path;

2. A detection system for analyzing one or more particles, comprising:

a first fluid power source for powering a flow of liquid in the fluid path;

3. The detection system of claim 1 or 2, further comprising:

a first gating means having an outlet, a drain and an inlet, for switching to communicate the outlet with the inlet; switching to communicate the outlet with the liquid outlet; the outlet of the first gating device is communicated with the inlet of the detection chamber, the inlet of the first gating device is connected with the sampling needle, and the liquid discharge port of the first gating device is used for discharging liquid to the waste liquid recovery device; the first fluid power source is in communication with an outlet of the detection chamber.

4. The detection system of claim 1 or 2, further comprising:

a second gating means having a drain port; the first fluid power source is connected with an outlet of the detection chamber through a second gating device, and the liquid discharge port is used for discharging liquid to the waste liquid recovery device; the second gating means is for switching the outlet of the detection chamber to communicate with the first source of fluid power and for switching the drain to communicate with the first source of fluid power.

5. A test system according to claim 1 or claim 2, wherein the first fluid power source has a drain for draining liquid towards a waste recovery device.

6. The detection system of claim 3, further comprising:

a second gating means; the first fluid power source is connected with the outlet of the detection chamber through the second gating device; the second gating device is also communicated with a cleaning solution container for providing cleaning solution through a third pipeline;

a second gating means is used to communicate at least one of the third conduit and the detection chamber with the first source of fluid power.

7. The detection system of claim 1 or 2, further comprising:

a second gating device with a liquid outlet, wherein the first fluid power source is connected with the outlet of the detection chamber through the second gating device, and the liquid outlet is used for discharging liquid to the waste liquid recovery device; the second gating device is used for communicating the liquid discharge port with the outlet of the detection chamber through switching, or communicating the liquid discharge port with the first fluid power source through switching; the second gating means is further for switching the outlet of the detection chamber into communication with the first source of fluid power;

a second fluid power source for powering the flow of liquid in the fluid path;

a first gating means having a first outlet, a second outlet and an inlet; the first outlet of the first gating device is communicated with the inlet of the detection chamber, the inlet of the first gating device is connected with the sampling needle, and the second outlet of the first gating device is communicated with the second fluid power source;

the first gating device is at least used for communicating the inlet with the first outlet through switching and communicating the first outlet with the second outlet through switching; or the first outlet of the first gating device is communicated with the inlet of the first gating device, and the first gating device is used for controlling the communication and disconnection between the first outlet of the first gating device and the second outlet of the first gating device; or the first outlet of the first gating device is communicated with the second outlet of the first gating device, and the first gating device is used for controlling the communication and disconnection between the inlet of the first gating device and the first outlet of the first gating device.

8. A detection system for analyzing one or more particles, comprising:

a second fluid power source for powering the flow of liquid in the fluid path;

9. A detection system for analyzing one or more particles, comprising:

a second fluid power source for powering the flow of liquid in the fluid path;

the sampling needle is used for sucking a sample by depending on the power provided by the first fluid power source when in a sample sucking position and sucking the cleaning liquid by depending on the power provided by the first fluid power source or the second fluid power source when in a sample sucking position; the sampling needle is cleaned in a manner that the second fluid power source sucks cleaning liquid;

10. A test system according to claim 8 or 9 wherein the first gating means has a drain for draining liquid towards a waste recovery means.

11. The detection system according to claim 8 or 9, further comprising:

a second gating device with a liquid outlet, wherein the first fluid power source is connected with the outlet of the detection chamber through the second gating device, and the liquid outlet is used for discharging liquid to the waste liquid recovery device; the second gating device is used for communicating the liquid discharge port with the outlet of the detection chamber through switching, or communicating the liquid discharge port with the first fluid power source through switching; the second gating means is also for switching the outlet of the detection chamber into communication with the first source of fluid power.

12. A test system as claimed in claim 2 or 9 wherein the sample and liquid draw levels are at different stations or at the same station.

13. A detection system for analyzing one or more particles, comprising:

a second fluid power source for powering the flow of liquid in the fluid path;

the sampling needle is used for sucking a sample depending on power provided by the first fluid power source and sucking cleaning liquid depending on power provided by the first fluid power source or the second fluid power source; the sampling needle is cleaned in a manner that the second fluid power source sucks cleaning liquid;

14. A liquid-phase suspension chip tester comprising a test system for analyzing one or more particles according to any of claims 1-13.