CN212364016U

CN212364016U - Leukocyte detection device

Info

Publication number: CN212364016U
Application number: CN202020143110.4U
Authority: CN
Inventors: 邹继华; 吴飞涯; 侯天文; 张文俊; 陈海云; 马东栋; 方亮
Original assignee: Ningbo Medical System Biotechnology Co Ltd
Current assignee: Ningbo Medical System Biotechnology Co Ltd
Priority date: 2020-01-22
Filing date: 2020-01-22
Publication date: 2021-01-15
Anticipated expiration: 2030-01-22

Abstract

The utility model relates to a technical field of blood detecting instrument, the utility model provides a leucocyte detection device, including preceding light subassembly, flow room subassembly, to the preceding receiving element, side direction receiving element, preceding light subassembly is used for providing the laser facula, flow room subassembly is used for providing detection channel, the diluent forms the sheath flow in detection channel, blood sample flows through detection channel's central authorities, it is preceding scattered light to be used for detecting to preceding receiving element, side direction receiving element is used for detecting side direction scattered light, preceding light subassembly, flow room subassembly, to the preceding receiving element, all be provided with at least one adjusting position's knob on the side direction receiving element. The position adjustment knob allows for precise and easy adjustment of the position of the front light assembly, the flow chamber assembly, the forward receiving member, and the lateral receiving member.

Description

Leukocyte detection device

Technical Field

The utility model relates to a blood detecting instrument's technical field, more exactly relate to a leucocyte detection device.

Background

Current routine blood detection typically uses optical methods that use multidimensional information of blood cells to classify and count the cells. The principle of leukocyte detection is shown in fig. 5, when a certain amount of blood cells are sucked into the detecting instrument and acted by a specific reagent, a blood sample is injected into a conical flow chamber filled with diluent through a nozzle, and the cells singly pass through the center of the flow chamber in a row under the wrapping of sheath fluid formed by the diluent; when blood cells suspended in the sheath fluid pass through the laser detection area after being accelerated twice, the blood cells are irradiated by the laser beam, and the properties of the generated scattered light are related to the cell size, the cell membrane and the refractive index of the internal structure of the cell. Wherein, the small angle forward scattering light reflects the size of the cell, the medium angle forward scattering light reflects the complexity of the substances in the cell membrane, and the side scattering light reflects the leafiness and the change of the cell membrane, nuclear model and cytoplasm. The photodiode receives the scattered signals and converts the scattered signals into electric pulses, and a three-dimensional distribution diagram, called as a scattergram, of the size of the blood cells and the information in the cells can be obtained according to the collected photoelectric pulse data, as shown in fig. 6. The instrument that detects leucocyte at present comprises preceding light subassembly, flow room subassembly, preceding receiving element, side direction receiving element etc. usually, when carrying out leucocyte detection based on the optics method, the position precision requirement between each part is very high, but current instrument is difficult to transfer when adjusting the position between each part, and some positions can only rely on the machining precision to guarantee, and the place of fine setting is not meticulous enough, is difficult to transfer the detection position of best.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide a leucocyte detection device makes when carrying out leucocyte detection based on the optical method, can adjust the position between parts such as preceding light subassembly, flow room subassembly, preceding receiving element, side direction receiving element accurately portably.

The technical solution of the utility model is, a leucocyte detection device is provided, including preceding light subassembly, flow room subassembly, preceding receiving element, side direction receiving element, preceding light subassembly is used for providing the laser facula, flow room subassembly is used for providing detection channel, the diluent forms the sheath flow in detection channel, blood sample flows through detection channel's central authorities, preceding receiving element is used for detecting preceding scattered light, side direction receiving element is used for detecting side direction scattered light, preceding light subassembly, flow room subassembly, preceding receiving element, all be provided with at least one adjusting position's knob on the side direction receiving element.

Compared with the prior art, the utility model discloses a leucocyte detection device has following advantage: the front light assembly, the flow chamber assembly, the front receiving component and the side receiving component are provided with position adjusting knobs, and the positions of the front light assembly, the flow chamber assembly, the front receiving component and the side receiving component can be accurately, simply and conveniently adjusted.

Preferably, the front light assembly comprises a laser, an aspheric lens, a small cylindrical mirror and a large cylindrical mirror, laser emitted by the laser is shaped by the aspheric lens, the small cylindrical mirror and the large cylindrical mirror and then is perpendicular to the flow direction of the blood sample and irradiates the center of the flow chamber, and a first adjusting knob arranged on the front light assembly is used for adjusting the front-back distance from the laser to the flow chamber. By adopting the structure, the front-back distance from the laser to the flow chamber can be accurately adjusted, and the laser spot is ensured to irradiate the center of the flow chamber.

Preferably, the front light assembly further comprises a temperature control assembly, and the temperature control assembly comprises a temperature sensor, a thermal protector and an electric heating rod. By adopting the structure, the optical power output by the laser is not influenced by the ambient temperature, and the accuracy of the whole measuring result is improved.

Preferably, the laser emits a laser wavelength of 635nm, and the size of the spot formed at the center of the flow cell is 300um × 20 um. By adopting the structure, the light spot is small, the light energy is concentrated, and the tested result is accurate.

Preferably, the forward receiving part is provided with a fourth adjusting knob and a fifth adjusting knob which are respectively used for adjusting the vertical position and the horizontal position of the light spot, the aperture receiving small angle of the forward receiving part is 2-6 degrees, and the receiving medium angle is 8-23 degrees. By adopting the structure, the vertical and horizontal positions of the light spot can be accurately, simply and conveniently adjusted, and the light spot is ensured to be aligned to the central position of the forward receiving part.

Preferably, the lateral receiving part is provided with a second adjusting knob and a third adjusting knob which are respectively used for adjusting the horizontal position and the vertical position of the light spot, and the aperture receiving angle of the lateral receiving part is 90 degrees. By adopting the structure, the vertical and horizontal positions of the light spot can be accurately, simply and conveniently adjusted, and the light spot is ensured to be aligned to the central position of the lateral receiving part.

Preferably, the leukocyte detection device further comprises a lateral light-focusing component for collecting lateral scattered light signals. By adopting the structure, the laterally weak scattered light signals are collected by the lateral light-gathering component and are beneficial to being captured by the lateral receiving component.

Preferably, a sixth adjusting knob is provided on the flow chamber assembly for adjusting the front and rear positions of the flow chamber. By adopting the structure, the position of the center of the flow chamber relative to the laser can be accurately adjusted, and the light spot of the laser is ensured to irradiate the center of the flow chamber.

Preferably, the leukocyte measurement device further comprises a dust-proof housing, and all components of the leukocyte measurement device are mounted in the dust-proof housing. With this structure, the optical device in the leukocyte detection device can be prevented from being exposed to the outside.

Drawings

Fig. 1 is a schematic view of the external structure of the leukocyte detection device of the present invention.

Fig. 2 is an assembly diagram of the leukocyte detection device of the present invention.

Fig. 3 is a schematic structural diagram of a front light component of the leukocyte detection device according to the present invention.

Fig. 4 is a schematic light path diagram of the leukocyte detection device of the present invention.

Fig. 5 is a schematic diagram of the operation principle of leukocyte detection.

Fig. 6 is a statistical diagram regarding the type and number of leukocytes.

As shown in the figure: 1. a dustproof shell, 2, a mounting bracket, 3, a temperature control component, 3-1, a temperature sensor, 3-2, a thermal protector, 3-3, an electric heating rod, 4, a front light component, 4-1, a lens fixing seat, 4-2, a laser, 4-3, an aspheric lens, 4-4, a small cylindrical mirror, 4-5, a large cylindrical mirror, 4-6, the device comprises a front light assembly fixing seat, 4-7, a first adjusting knob, 5, a lateral receiving component, 5-1, a second adjusting knob, 5-2, a third adjusting knob, 6, a lateral light gathering component, 7, a forward receiving component, 7-1, a fourth adjusting knob, 7-2, a fifth adjusting knob, 8, a flow chamber component, 8-1, a sixth adjusting knob, 9, a flow chamber support, 10 and an optical bottom plate.

Detailed Description

For a better understanding of the present application, various aspects of the present application will be described in more detail with reference to the accompanying drawings. It should be understood that the detailed description is merely illustrative of exemplary embodiments of the present application and does not limit the scope of the present application in any way. Like reference numerals refer to like elements throughout the specification.

In the drawings, the thickness, size, and shape of an object have been slightly exaggerated for convenience of explanation. The figures are purely diagrammatic and not drawn to scale.

It will be further understood that the terms "comprises," "comprising," "includes," "including," and/or "including," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. Further, when a statement such as "… at least one" appears after the list of listed features, the entire listed feature is modified rather than modifying individual elements in the list.

As shown in fig. 1 to fig. 3, the leukocyte detection device of the present invention includes a dust-proof housing 1, a mounting bracket 2 is disposed at the bottom of the dust-proof housing 1, and the mounting bracket 2 is used for mounting the leukocyte detection device of the present invention in other blood detection instruments. An optical bottom plate 10 is arranged in the dust-proof housing 1, a front light assembly 4, a flow chamber assembly 8, a front receiving component 7, a side receiving component 5 and a side light gathering component 6 are arranged on the optical bottom plate 10, the front light assembly 4 is fixed on the optical bottom plate 10 through front light component fixing seats 4-6, the flow chamber assembly 8 is fixed on the optical bottom plate 10 through a flow chamber support 9, the front light assembly 4, the flow chamber assembly 8 and the front receiving component 7 are arranged in a straight line, and the side receiving component 5, the side light gathering component 6 and the flow chamber assembly 8 are also arranged in a straight line. The flow chamber assembly 8 is used to provide a detection channel in which the diluent forms a sheath flow and through the center of which the blood sample flows. The front light assembly 4 comprises a laser 4-2, an aspheric lens 4-3, a small cylindrical mirror 4-4 and a large cylindrical mirror 4-5, the laser 4-2, the aspheric lens 4-3, the small cylindrical mirror 4-4 and the large cylindrical mirror 4-5 are all arranged in a lens fixing seat 4-1, and laser emitted by the laser 4-2 is shaped by the aspheric lens 4-3, the small cylindrical mirror 4-4 and the large cylindrical mirror 4-5 and then is perpendicular to the flow direction of the blood sample and irradiates the center of the flow chamber; the front light assembly 4 is provided with a first adjusting knob 4-7 for adjusting the front-back distance from the laser to the flow chamber; the front light assembly 4 is further provided with a temperature control assembly 3, the temperature control assembly 3 comprises a temperature sensor 3-1, a thermal protector 3-2 and an electric heating rod 3-3, and the temperature control assembly 3 is used for keeping the front light assembly 4 in a constant temperature state. The forward receiving part 7 is provided with a fourth adjusting knob 7-1 and a fifth adjusting knob 7-2 which are respectively used for adjusting the vertical position and the horizontal position of the light spot to ensure that the light spot is aligned with the central position of the forward receiving part. The lateral receiving component 5 is provided with a second adjusting knob 5-1 and a third adjusting knob 5-2 which are respectively used for adjusting the horizontal position and the vertical position of the light spot to ensure that the light spot is aligned with the central position of the lateral receiving component. The side light-gathering part 6 is used for collecting weak signals of side scattered light, so that the side light-receiving part can capture the side scattered light. The flow chamber assembly 8 is provided with a sixth adjusting knob 8-1 for adjusting the front and rear positions of the flow chamber, so that the position of the center of the flow chamber relative to the laser can be accurately adjusted, and the laser spot can be ensured to irradiate the center of the flow chamber. In the embodiment, the laser wavelength emitted by the laser 4-2 is 635nm, and the size of a light spot formed in the center of the flow chamber is 300um multiplied by 20 um; the aperture of the forward receiving part 7 receives a small angle of 2-6 degrees and a medium angle of 8-23 degrees; the diaphragm receiving angle of the lateral receiving part 5 is 90 °.

The working principle of the leucocyte detection device of the utility model is shown in fig. 4 to fig. 6, the first adjusting knob and the sixth adjusting knob are adjusted to make the facula of the laser irradiate the center of the flow chamber; after a blood sample is injected into a conical flow chamber filled with diluent, cells singly pass through the center of the flow chamber in a row under the wrapping of sheath liquid formed by the diluent, when blood cells suspended in the sheath liquid pass through a laser detection area after being accelerated for the second time, the blood cells are irradiated by laser beams, and the properties of generated scattered light are related to the size of the cells and the refractive indexes of cell membranes and internal structures of the cells; adjusting a fourth adjusting knob and a fifth adjusting knob to enable the light spots to be aligned to the central position of the forward receiving component, and receiving small-angle forward scattering light reflecting the size of the cells and medium-angle forward scattering light reflecting the complexity of substances in the cell membrane by a photodiode in the forward receiving component; adjusting the second adjusting knob and the third adjusting knob to enable the light spots to be aligned to the central position of the lateral receiving component, and receiving lateral scattered light reflecting the change of the leaf separation property and the cell membrane, the nuclear model and the cytoplasm by a photodiode of the lateral receiving component; the photodiode converts the received scattered light signals into electric pulses, and then obtains a three-dimensional distribution diagram of the sizes of the white blood cells and the internal information of the cells according to the collected photoelectric pulse data.

Claims

1. A white blood cell detection device comprises a front light assembly (4), a flow chamber assembly (8), a front receiving component (7) and a side receiving component (5), wherein the front light assembly (4) is used for providing laser spots, the flow chamber assembly (8) is used for providing detection channels, diluent forms sheath flow in the detection channels, a blood sample flows through the center of the detection channels, the front receiving component (7) is used for detecting front scattered light, and the side receiving component (5) is used for detecting side scattered light, and the white blood cell detection device is characterized in that at least one knob for adjusting the position is arranged on each of the front light assembly (4), the flow chamber assembly (8), the front receiving component (7) and the side receiving component (5).

2. The leukocyte detection device according to claim 1, wherein the front light assembly (4) comprises a laser (4-2), an aspheric lens (4-3), a small cylindrical mirror (4-4) and a large cylindrical mirror (4-5), the laser emitted by the laser (4-2) is shaped by the aspheric lens (4-3), the small cylindrical mirror (4-4) and the large cylindrical mirror (4-5) and then perpendicular to the flow direction of the blood sample and irradiates the center of the flow chamber, and a first adjusting knob (4-7) is arranged on the front light assembly (4) and used for adjusting the front-back distance from the laser to the flow chamber.

3. The white blood cell detection device according to claim 2, wherein the front light assembly (4) further comprises a temperature control assembly (3), and the temperature control assembly (3) comprises a temperature sensor (3-1), a thermal protector (3-2) and an electric heating rod (3-3).

4. The leukocyte detection apparatus according to claim 2, wherein the laser (4-2) emits a laser wavelength of 635nm, and a spot size formed at the center of the flow cell is 300um x 20 um.

5. The white blood cell detecting device according to claim 1, wherein the forward receiving part (7) is provided with a fourth adjusting knob (7-1) and a fifth adjusting knob (7-2) for adjusting the vertical and horizontal positions of the light spot, respectively, and the diaphragm of the forward receiving part (7) receives a small angle of 2 ° to 6 ° and a medium angle of 8 ° to 23 °.

6. The white blood cell detecting apparatus according to claim 1, wherein the lateral receiving part (5) is provided with a second adjusting knob (5-1) and a third adjusting knob (5-2) for adjusting the horizontal and vertical positions of the light spot, respectively, and the diaphragm receiving angle of the lateral receiving part (5) is 90 °.

7. The leukocyte detection device according to claim 1, further comprising a lateral light focusing element (6), the lateral light focusing element (6) being configured to collect laterally scattered light signals.

8. The white blood cell detecting apparatus according to claim 1, wherein a sixth adjusting knob (8-1) is provided on the flow cell assembly (8) for adjusting a front-rear position of the flow cell.

9. The white blood cell detection device according to claim 1, further comprising a dust-proof housing (1), all components of the white blood cell detection device being mounted in the dust-proof housing (1).