CN212335213U - Adsorption film installation device of nucleic acid recovery column - Google Patents
Adsorption film installation device of nucleic acid recovery column Download PDFInfo
- Publication number
- CN212335213U CN212335213U CN202021468966.5U CN202021468966U CN212335213U CN 212335213 U CN212335213 U CN 212335213U CN 202021468966 U CN202021468966 U CN 202021468966U CN 212335213 U CN212335213 U CN 212335213U
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- Prior art keywords
- nucleic acid
- acid recovery
- drive assembly
- recovery column
- adsorption film
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 101
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 101
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 101
- 238000011084 recovery Methods 0.000 title claims abstract description 92
- 238000001179 sorption measurement Methods 0.000 title claims abstract description 48
- 238000009434 installation Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims description 16
- 230000000903 blocking effect Effects 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims description 8
- 230000033001 locomotion Effects 0.000 claims description 5
- 230000004888 barrier function Effects 0.000 claims 3
- 238000001125 extrusion Methods 0.000 abstract description 19
- 230000000694 effects Effects 0.000 abstract description 5
- 238000012856 packing Methods 0.000 abstract description 3
- 239000013612 plasmid Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an adsorption film installation device of nucleic acid recovery post, including first drive assembly, second drive assembly and strutting arrangement, first drive assembly and second drive assembly set up relatively, strutting arrangement is located between first drive assembly and the second drive assembly, strutting arrangement is used for placing the nucleic acid recovery post, first drive assembly includes first driver part and first material pushing component, first driver part is used for driving first material pushing component and withstands the one end of nucleic acid recovery post, second drive assembly is equipped with second driver part and second material pushing component, second driver assembly is arranged in driving the second material pushing component and stretches into in the nucleic acid recovery post with the top and push away the adsorption film along the other end of nucleic acid recovery post. This technical scheme has avoided because of the inconsistent problem of the extrusion degree that artifical packing adsorption film leads to through setting up first drive assembly and second drive assembly, can guarantee the extrusion precision of adsorption film, ensures the extrusion effect, improves the installation effectiveness, avoids causing the pollution to nucleic acid recovery post.
Description
Technical Field
The utility model is used for nucleic acid draws and fills out membrane technical field, especially relates to an adsorption film installation device of nucleic acid recovery post.
Background
Nucleic acids are smaller DNA molecules that are capable of autonomous replication that are attached to the DNA of the noncellular chromosome or nuclear region of a cell. Most plasmids are in a circular configuration. Plasmids are present in many organisms such as bacteria and yeasts, and even in the organelles such as chloroplasts and mitochondria of plants. The DNA of the native plasmid ranges in length from thousands of base pairs to hundreds of thousands of base pairs. One or more nucleic acids may be present in a cell at the same time. Nucleic acid is the most common carrier in genetic engineering, and can be expressed by modifying a plasmid with restriction endonuclease, inserting a target gene into the modified plasmid, and entering a host cell by means of transformation or transduction. Therefore, the extraction of nucleic acids is one of the basic molecular biotechnology, and many studies in the field of molecular biotechnology require the extraction of nucleic acids of high quality. The extraction of nucleic acid usually requires a large number of nucleic acid recovery columns, the top of which is connected with a lid for sealing, and the bottom of which is provided with an adsorption membrane. When extracting nucleic acid, the adsorption film can adsorb nucleic acid.
In the prior art, the adsorption membrane is usually filled into the bottom of the nucleic acid recovery column by a manual filling method, which easily causes inconsistent extrusion degree of the adsorption membrane, thereby causing inaccurate recovery of nucleic acid in the use process of the nucleic acid recovery column, and the manual filling speed is slow, which easily causes pollution to the inside of the nucleic acid recovery column.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to solve one of the technical problem that exists among the prior art at least, provide an absorption membrane installation device of nucleic acid recovery post, its extrusion precision that can guarantee the absorption membrane ensures the extrusion effect, improves the installation effectiveness, avoids causing the pollution to nucleic acid recovery post.
The utility model provides a technical scheme that its technical problem adopted is:
the utility model provides an adsorption film installation device of nucleic acid recovery post, includes first drive assembly, second drive assembly and strutting arrangement, first drive assembly and second drive assembly set up relatively, strutting arrangement is located between first drive assembly and the second drive assembly, strutting arrangement is used for placing nucleic acid recovery post, first drive assembly includes first driver part and first material pushing component, first driver part is used for the drive first material pushing component withstands the one end of nucleic acid recovery post, second drive assembly is equipped with second driver part and second material pushing component, second driver assembly is used for the drive the second material pushing component stretches into in the nucleic acid recovery post with the top pushes away the adsorption film along the other end of nucleic acid recovery post.
In combination with the foregoing implementation manner, in certain implementations of the present invention, the first material pushing component includes a first connecting rod, the first driving component includes a motor, an output end of the motor is provided with a cam, the cam is connected to a second connecting rod, the first driving component further includes a sliding sleeve, the first connecting rod is slidably mounted in the sliding sleeve, and the first connecting rod is hinged to the second connecting rod.
In combination with the foregoing implementation manner, in certain implementation manners of the present invention, the second driving assembly further includes a fixing base, the second driving component includes a first cylinder, the first cylinder is installed in the fixing base, the second pushing component includes an extrusion rod, the extrusion rod is connected with the output shaft of the first cylinder.
In combination with the above implementation manner, the utility model discloses an in certain implementation, still include the subassembly of unloading and block parts, the subassembly of unloading is located the top of stripper rod, the subassembly of unloading includes the second cylinder and follows the push rod of unloading of arranging from top to bottom, the output of second cylinder with the push rod of unloading is connected, block parts is located the below of stripper rod, when the push rod of unloading promotes nucleic acid recovery post downstream, block parts can support the lid of nucleic acid recovery post and make it compress tightly in the opening of nucleic acid recovery post.
In combination with the above implementation manner, in certain implementation manners of the present invention, the discharging component is installed on the fixing base.
In combination with the above implementations, in certain implementations of the invention, the blocking member has an inclined surface for guiding movement of a lid of the nucleic acid recovery column.
In combination with the above implementation manner, in certain implementation manners of the present invention, the supporting device further includes an oscillator, the oscillator has a current track that is spiral ascending, and the current track is located in the outlet of the discharging component.
One of the above technical solutions has at least one of the following advantages or beneficial effects: when the adsorption film is installed in the nucleic acid recovery column, the adsorption film is firstly placed in the nucleic acid recovery column, then the nucleic acid recovery column is placed in the supporting device, the first pushing component is driven to push against one end of the nucleic acid recovery column through the first driving component, the second pushing component is driven by the second driving component to extend into the nucleic acid recovery column along the other end of the nucleic acid recovery column so as to push the adsorption film, and then the work of filling the adsorption film can be completed. This technical scheme's nucleic acid recovery column's adsorption film installation device has avoided because of the inconsistent problem of the extrusion degree that artifical packing adsorption film leads to through the setting of first drive assembly and second drive assembly, can guarantee the extrusion precision of adsorption film, ensures the extrusion effect, improves the installation effectiveness, avoids causing the pollution to nucleic acid recovery column.
Drawings
The present invention will be further explained with reference to the accompanying drawings:
FIG. 1 is a schematic structural view of a first drive assembly, a second drive assembly, a discharge assembly and a blocking member according to an embodiment of the present invention;
fig. 2 is a schematic diagram of an embodiment of the oscillator according to the present invention.
Detailed Description
This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.
In the present invention, if there is a description of directions (up, down, left, right, front and back), it is only for convenience of description of the technical solution of the present invention, and it is not intended to indicate or imply that the technical features indicated must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the utility model, the meaning of a plurality of is one or more, the meaning of a plurality of is more than two, and the meaning of more than two is understood as not including the number; the terms "above", "below", "within" and the like are understood to include the instant numbers. In the description of the present invention, if there is any description of "first" and "second" only for the purpose of distinguishing technical features, it is not to be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the precedence of the indicated technical features.
In the present invention, unless otherwise explicitly defined, the terms "set", "install", "connect", and the like are to be understood in a broad sense, and for example, may be directly connected or may be indirectly connected through an intermediate medium; can be fixedly connected, can also be detachably connected and can also be integrally formed; may be mechanically coupled, may be electrically coupled or may be capable of communicating with each other; either as communication within the two elements or as an interactive relationship of the two elements. The technical skill in the art can reasonably determine the specific meaning of the above words in the present invention by combining the specific contents of the technical solution.
Referring to fig. 1 and fig. 2, the embodiment of the utility model provides an adsorption film installation device of nucleic acid recovery post, including first drive assembly, second drive assembly and strutting arrangement 1, first drive assembly and second drive assembly set up relatively, strutting arrangement 1 is located between first drive assembly and the second drive assembly, strutting arrangement 1 is used for placing nucleic acid recovery post, first drive assembly includes first drive component and first material pushing component 21, first drive component is used for driving first material pushing component 21 and withstands the one end of nucleic acid recovery post 3, second drive assembly is equipped with second drive component and second material pushing component 41, second drive assembly is used for driving second material pushing component 41 and stretches into in the nucleic acid recovery post 3 with the top and push away the adsorption film along the other end of nucleic acid recovery post 3.
When the adsorption film is installed in the nucleic acid recovery column 3, the adsorption film is firstly placed in the nucleic acid recovery column 3, then the nucleic acid recovery column 3 is placed in the supporting device 1, the first pushing component 21 is driven by the first driving component to push against one end of the nucleic acid recovery column 3, the second pushing component 41 is driven by the second driving component to extend into the nucleic acid recovery column 3 along the other end of the nucleic acid recovery column 3 so as to push the adsorption film, and then the work of filling the adsorption film can be completed. This technical scheme's nucleic acid recovery column's adsorption film installation device has avoided because of the inconsistent problem of the extrusion degree that artifical packing adsorption film leads to through the setting of first drive assembly and second drive assembly, can guarantee the extrusion precision of adsorption film, ensures the extrusion effect, improves the installation effectiveness, avoids leading to the fact the pollution to nucleic acid recovery column 3.
The first driving component can drive the first pushing component 21 to move by adopting a crank link mechanism, cylinder driving, linear motor driving or other modes. Referring to fig. 1, in some embodiments, the first pushing member 21 includes a first connecting rod, the first driving member includes a motor, an output end of the motor is provided with a cam 22, the cam 22 is connected with a second connecting rod 24, the first driving assembly further includes a sliding sleeve 25, the first connecting rod is slidably mounted in the sliding sleeve 25, and the first connecting rod is hinged with the second connecting rod 24. The motor drives the cam 22 to rotate, and then the cam 22 drives the second connecting rod 24 to slide along the sliding sleeve 25 through the first connecting rod, so that the first pushing component 21 can push against the nucleic acid recovery column 3, and when the second pushing component 41 extends into the nucleic acid recovery column 3 to push against the adsorption membrane, the nucleic acid recovery column 3 can be supported, and the nucleic acid recovery column 3 is prevented from moving.
Similarly, the second driving assembly may also drive the second pushing member 41 to move by using a crank-link mechanism, a cylinder drive, or a linear motor drive. Referring to fig. 1, in some embodiments, the second driving assembly further includes a fixing seat 42, the second driving component includes a first air cylinder 43, the first air cylinder 43 is mounted on the fixing seat 42, and the second pushing component 41 includes a pressing rod, and the pressing rod is connected with an output shaft of the first air cylinder 43. The cylinder drives the extrusion rod to reciprocate so as to extend into the nucleic acid recovery column 3 to push the adsorption membrane. Through first drive assembly and the cooperation of second drive assembly use, can accomplish the work of filling the adsorption film, avoided filling the inconsistent problem of extrusion degree that the adsorption film leads to because of the manual work, improve the extrusion precision.
After pressing the adsorption membrane into the nucleic acid recovery column 3, the lid 31 can be closed to prevent the internal environment of the nucleic acid recovery column 3 from being contaminated. Referring to FIG. 1, in some embodiments, the adsorption membrane mounting apparatus for a nucleic acid recovery column further comprises a discharge assembly and a blocking member 5, the discharge assembly is located above the extrusion rod, the discharge assembly comprises a second cylinder 61 and a discharge push rod 62 arranged in the up-down direction, the output end of the second cylinder 61 is connected to the discharge push rod 62, and the second cylinder 61 can drive the discharge push rod 62 to move in the up-down direction. The blocking component 5 is positioned below the extrusion rod, when the unloading push rod 62 pushes the nucleic acid recovery column 3 to move downwards, the blocking component 5 can prop against the cover 31 of the nucleic acid recovery column 3 and press the cover 31 against the opening of the nucleic acid recovery column 3, so that the adsorption film mounting device of the nucleic acid recovery column 3 can automatically close the cover 31 after the adsorption film is filled, and the working efficiency is improved.
The nucleic acid recovery column 3 is connected to the lid 31 of the nucleic acid recovery column 3, as shown in FIG. 2. Referring to FIG. 1, in some embodiments, the blocking member 5 has an inclined surface 51, and the inclined surface 51 serves to guide the movement of the lid 31 of the nucleic acid recovery column 3. When the discharge push rod 62 pushes the nucleic acid recovery column 3 to move downward, the cover 31 of the nucleic acid recovery column 3 first contacts the inclined surface 51 and is attached to the opening of the nucleic acid recovery column 3 under the guidance of the inclined surface 51 to close the opening of the nucleic acid recovery column 3.
Referring to fig. 1, in some embodiments, the discharging assembly is mounted on the fixing base 42, so that the discharging assembly is convenient to mount and space-saving.
Referring to fig. 2, in some embodiments, the supporting device 1 further includes an oscillator, the oscillator has a passing rail 11 that rises spirally, an outlet 12 of the passing rail 11 is located below the discharging assembly, the passing rail 11 is provided with a bottom plate that can move downwards at the outlet 12, and the nucleic acid recovery column 3 can slide downwards along the bottom plate under the push of the discharging assembly when running to the outlet 12. The nucleic acid recovery column 3 with the adsorption film placed in advance is placed into a vibrator, the nucleic acid recovery column 3 passes through the passing rail 11 under the action of the vibrator and reaches the outlet 12 of the passing rail 11, the nucleic acid recovery column is pushed downwards by the unloading assembly after being subjected to adsorption film pressing by the first driving assembly and the second driving assembly, the nucleic acid recovery column 3 slides downwards under the guidance of the bottom plate, the cover 31 is closed under the action of the blocking component 5, and the cover is automatically closed after the adsorption film pressing is completed. Meanwhile, when the nucleic acid recovery column 3 passes through the passing rail 11, the nucleic acid recovery column 3 shakes the nucleic acid recovery column 3 which does not meet the arrangement requirement back into the oscillator under the oscillation action and the gravity action of the oscillator, and conveys the nucleic acid recovery column 3 with the downward cover 31 which meets the arrangement requirement to the outlet 12 of the passing rail 11, so as to perform the next operation. Wherein, the bottom plate can adopt stainless steel material, and when the subassembly of unloading pushed the downward motion of nucleic acid recovery post 3, the bottom plate can be under the effect of nucleic acid recovery post 3 downward sloping to guide the downward motion of nucleic acid recovery post 3.
In the description herein, references to the description of the term "example," "an embodiment," or "some embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The invention is not limited to the above embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the invention, and such equivalent modifications or substitutions are included in the scope defined by the claims of the present application.
Claims (7)
1. The utility model provides an adsorption film installation device of nucleic acid recovery post which characterized in that: including first drive assembly, second drive assembly and strutting arrangement, first drive assembly and second drive assembly set up relatively, strutting arrangement is located between first drive assembly and the second drive assembly, strutting arrangement is used for placing nucleic acid recovery post, first drive assembly includes first driver part and the first material part that pushes away, first driver part is used for the drive first material part that pushes away withstands the one end of nucleic acid recovery post, second drive assembly is equipped with second driver part and second and pushes away the material part, second drive assembly is used for the drive the second pushes away the material part and stretches into in the nucleic acid recovery post with the top in the other end of nucleic acid recovery post with the top to push away the adsorption film.
2. The nucleic acid recovery column adsorption membrane mounting device according to claim 1, wherein: the first material pushing component comprises a first connecting rod, the first driving component comprises a motor, a cam is arranged at the output end of the motor and connected with a second connecting rod, the first driving component further comprises a sliding sleeve, the first connecting rod is slidably installed in the sliding sleeve, and the first connecting rod is hinged to the second connecting rod.
3. The nucleic acid recovery column adsorption membrane mounting device according to claim 1, wherein: the second driving assembly further comprises a fixing seat, the second driving component comprises a first air cylinder, the first air cylinder is installed on the fixing seat, the second pushing component comprises a squeezing rod, and the squeezing rod is connected with an output shaft of the first air cylinder.
4. The apparatus for mounting an adsorption membrane of a nucleic acid recovery column according to claim 3, wherein: still include the subassembly of unloading and barrier member, the subassembly of unloading is located the top of stripper bar, the subassembly of unloading includes the second cylinder and follows the push rod of unloading of arranging from top to bottom, the output of second cylinder with the push rod of unloading is connected, the barrier member is located the below of stripper bar, when the push rod of unloading promotes nucleic acid recovery post and moves down, the barrier member can support the lid of nucleic acid recovery post and make it compress tightly in the opening of nucleic acid recovery post.
5. The apparatus for mounting an adsorption membrane of a nucleic acid recovery column according to claim 4, wherein: the discharging assembly is arranged on the fixed seat.
6. The apparatus for mounting an adsorption membrane of a nucleic acid recovery column according to claim 4, wherein: the blocking member has an inclined surface for guiding the movement of the lid of the nucleic acid recovery column.
7. The apparatus for mounting an adsorption membrane of a nucleic acid recovery column according to claim 4, wherein: the supporting device further comprises an oscillator, the oscillator is provided with a passing track which spirally rises, and an outlet of the passing track is located below the discharging assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021468966.5U CN212335213U (en) | 2020-07-23 | 2020-07-23 | Adsorption film installation device of nucleic acid recovery column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021468966.5U CN212335213U (en) | 2020-07-23 | 2020-07-23 | Adsorption film installation device of nucleic acid recovery column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212335213U true CN212335213U (en) | 2021-01-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021468966.5U Withdrawn - After Issue CN212335213U (en) | 2020-07-23 | 2020-07-23 | Adsorption film installation device of nucleic acid recovery column |
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| CN (1) | CN212335213U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778241A (en) * | 2020-07-23 | 2020-10-16 | 广州捷瑞生物工程有限公司 | Adsorption film installation device of nucleic acid recovery column |
-
2020
- 2020-07-23 CN CN202021468966.5U patent/CN212335213U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778241A (en) * | 2020-07-23 | 2020-10-16 | 广州捷瑞生物工程有限公司 | Adsorption film installation device of nucleic acid recovery column |
| CN111778241B (en) * | 2020-07-23 | 2024-08-06 | 广州捷瑞生物工程有限公司 | Adsorption film installation device of nucleic acid recovery column |
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| AV01 | Patent right actively abandoned | ||
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| AV01 | Patent right actively abandoned |
Granted publication date: 20210112 Effective date of abandoning: 20240806 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20210112 Effective date of abandoning: 20240806 |