CN212316141U - Dynamic pressure tissue engineering valve cell inoculator - Google Patents
Dynamic pressure tissue engineering valve cell inoculator Download PDFInfo
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- CN212316141U CN212316141U CN202021940357.5U CN202021940357U CN212316141U CN 212316141 U CN212316141 U CN 212316141U CN 202021940357 U CN202021940357 U CN 202021940357U CN 212316141 U CN212316141 U CN 212316141U
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Abstract
The utility model relates to a developments pressurization tissue engineering valve cell inoculator, including upper base, upper cover, lower base, lower cover and valve anchor clamps, be equipped with the upper portion liquid chamber in the upper base, be equipped with the lower part liquid chamber in the lower base, be equipped with the upper portion air chamber in the upper cover, be equipped with the lower part air chamber in the lower cover, the upper cover with the upper pedestal connection, the upper portion air chamber with be equipped with the upper portion diaphragm between the upper portion liquid chamber, the lower cover with lower pedestal connection, the lower part air chamber with be equipped with the lower part diaphragm between the lower part liquid chamber, the valve anchor clamps set up in the upper portion liquid chamber, the upper base with the lower base passes through the buckle and connects. The utility model is used for the cell inoculation of human heart tissue engineering valve can realize the pressurization dynamic culture of seed cell, makes the even inoculation in the support material of seed cell to can get into valve support inside under the pressure effect, realize the comprehensive cellization of support, avoid the cell to drop.
Description
The technical field is as follows:
the utility model relates to the technical field of biomedical engineering, in particular to a dynamic pressurization tissue engineering valve cell inoculator.
Background art:
at present, the global heart disease incidence rate is increased year by year, the number of patients is increased continuously, the incidence rate of heart valve diseases is also in an increasing trend, and more than 30 ten thousand heart valve operations are performed every year in the world. The main surgical approach to heart valve surgery is valve replacement, and two valve substitutes are currently used clinically primarily: both mechanical and biological valves have their drawbacks. Mechanical valves require lifelong anticoagulation treatment, with the risk of bleeding and thromboembolism; bioprosthetic valves have a limited useful life of about 10-15 years and many patients are at risk for a secondary valve change. It is currently widely accepted that tissue engineered valves are the most promising ideal valve replacement because of their advantages of lifetime use without anticoagulation.
The tissue engineering valve is constructed by inoculating seed cells on a valve stent for in vitro culture, and implanting the seed cells into a body after forming a new extracellular matrix and a complete cell layer. However, the current tissue engineering valve is not applied to clinical application, and the main problems are the problems of seed cells and culture conditions. In the past, a static inoculation static culture mode is mostly studied, but the mode is simple to operate, but has the problems of nonuniform cell inoculation and low inoculation efficiency. Patent No. ZL200810232069.1 discloses that the collagen and elastin of acellular scaffolds themselves are crosslinked by epichlorohydrin (C3H5Cl), and simultaneously, rgd (YGRGDSP) polypeptide is linked to the collagen and elastin of acellular xenogenic valve/vascular scaffolds as tissue engineering valves/vascular scaffolds, so that the YGRGDSP polypeptide can be uniformly and firmly distributed throughout the acellular valve scaffolds, so as to enhance the adhesion of seed cells and scaffolds and stimulate cell proliferation. However, this method is not only complicated in steps, but also requires chemical treatment of the valve, and is not suitable for cell seeding of human heart valves. Patent No. ZL201720600607.2 is through setting up the cell reactor in the thermostated container, and the thermostated container internal fixation has driving motor, can drive the cell reactor through driving motor and rotate, makes the seeding cell evenly disperse in the cell reactor to disturb nutrient solution and cell in the cell reactor, make the cell evenly distributed in the cell reactor, in order to prevent the emergence of cell contact suppression phenomenon. However, the device can only realize the uniform distribution of cells, the dynamic pressurization of the cells cannot be realized, the tissue engineering valve cultured in vitro is difficult to grow a complete cell layer, the connection between the cells and the stent is loose and is limited to surface connection, once the tissue engineering valve is implanted into a human body, most of the cells can be circularly washed away by body fluid in the human body, and the device cannot play a role.
The utility model has the following contents:
technical problem to be solved
The utility model aims at providing a developments pressurization tissue engineering valve cell inoculator for human heart tissue engineering valve cell inoculation, among the solution prior art, the tissue engineering valve cell that carries out the external culture is loose with being connected of support, and the cell can be washed away by the body fluid circulation in the human body easily after implanting the human body, plays the problem that should have the effect.
The technical scheme is as follows:
in order to solve the technical problem, the utility model adopts the following technical scheme: a dynamic pressurization tissue engineering valve cell inoculator comprises an upper base, an upper cover, a lower base, a lower cover and a valve clamp, wherein an upper liquid chamber is arranged in the upper base, a lower liquid chamber is arranged in the lower base, an upper air chamber is arranged in the upper cover, a lower air chamber is arranged in the lower cover, the upper cover is connected with the upper base, an upper diaphragm is arranged between the upper air chamber and the upper liquid chamber, the lower cover is connected with the lower base, a lower diaphragm is arranged between the lower air chamber and the lower liquid chamber, the valve clamp is arranged in the upper liquid chamber, the upper base is connected with the lower base through a buckle, the upper liquid chamber is communicated with the lower liquid chamber, an upper liquid injection port and an upper air exhaust port which are communicated with the upper liquid chamber are arranged on the upper base, and an upper gas injection port which is communicated with the upper air chamber is arranged on the upper cover, the lower base is provided with a lower liquid pouring port and a lower exhaust port which are communicated with the lower liquid chamber, and the lower cover is provided with a lower gas pouring port which is communicated with the lower gas chamber.
The upper base and the lower base are detachably connected through the buckle, the upper liquid chamber is communicated with the lower liquid chamber, the valve clamp is used for clamping and fixing a sutured three-valve heart valve in the upper liquid chamber, the upper liquid injection port and the lower liquid injection port are respectively used for injecting cell suspensions into the upper liquid chamber and the lower liquid chamber, the upper air exhaust port and the lower air exhaust port are used for exhausting air in the upper liquid chamber and the lower liquid chamber when the cell suspensions are injected, the upper air injection port and the lower air exhaust port are used for inflating and pressurizing the upper air chamber and the lower air chamber through an air pump after the upper liquid chamber and the lower liquid chamber are filled with the cell suspensions, the upper diaphragm and the lower diaphragm deform under the action of air pressure to pressurize the cell suspensions in the upper liquid chamber and the lower liquid chamber, the heart valve seed cells are dynamically cultured in a cell suspension with certain pressure.
Further, valve anchor clamps include seam seat, solid fixed ring and support, gu fixed ring sets up the seam seat with between the support, the support with form the growth chamber between the fixed ring, be equipped with moulding ball in the growth chamber, the seam seat is used for sewing up heart valve's border, and imbeds in the growth chamber, moulding ball is used for pressing heart valve make on the support heart valve with the support laminating, subsequent tissue culture of being convenient for.
Furthermore, a rubber sealing ring is arranged on the joint surface of the upper base and the lower base, and the joint surface of the upper base and the lower base is sealed to prevent the leakage of the cell suspension.
Further, the upper diaphragm and the lower diaphragm are both convex diaphragms and protrude towards the upper liquid chamber and the lower liquid chamber respectively, so that the deformation directions of the upper diaphragm and the lower diaphragm are ensured when the upper air chamber and the lower air chamber are inflated and pressurized.
Furthermore, the upper cover and the lower cover are respectively provided with a pressure gauge communicated with the upper air chamber and the lower air chamber, and the pressure gauges are used for measuring the inflation pressure in the upper air chamber and the lower air chamber, so that the inflation and pressurization pressure can be conveniently controlled.
Further, be equipped with the thread groove in the upper portion liquid chamber, be equipped with the clamping ring in the thread groove, the outside of clamping ring be equipped with thread groove complex external screw thread, valve anchor clamps quilt the clamping ring is fixed in the upper portion liquid chamber, when guaranteeing valve anchor clamps's fixed effect, be convenient for follow take out in the upper portion liquid chamber valve anchor clamps.
Furthermore, the upper air injection port, the upper liquid injection port, the upper air exhaust port, the lower air injection port, the lower liquid injection port and the lower air exhaust port are respectively provided with a detachable threaded plug, so that the plugging is facilitated.
Furthermore, the upper diaphragm and the lower diaphragm are made of high-elasticity silica gel, so that the high-elasticity silica gel diaphragm has high corrosion resistance while ensuring enough elasticity, is not easy to age and is beneficial to long-term use.
(III) the beneficial effects are as follows:
compared with the prior art, the utility model discloses the beneficial effect who produces is: the upper liquid chamber and the lower liquid chamber are respectively separated from the upper air chamber and the lower air chamber through the upper diaphragm and the lower diaphragm, the valve clamp is arranged in the upper liquid chamber, the upper liquid chamber and the lower liquid chamber which are filled with cell suspension can be pressurized through inflating the upper air chamber and the lower air chamber, heart valve seed cells on the valve clamp are dynamically cultured in the cell suspension with certain pressure, the close fusion of the seed cells and the support is promoted, the comprehensive cellularization of the support is realized, and the inoculation effect of tissue engineering valve cells is ensured.
Description of the drawings:
in order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.
FIG. 1 is a schematic diagram of the overall structure of a dynamic pressurized tissue engineering valve cell inoculator according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a dynamic pressurized tissue engineering valve cell inoculator according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the upper base of a dynamic pressurized tissue engineering valve cell inoculator according to an embodiment of the present invention;
FIG. 4 is a schematic view of the lower base of a dynamic pressurized tissue engineering valve cell inoculator according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a valve clamp of a dynamic pressure tissue engineering valve cell inoculator according to an embodiment of the present invention;
FIG. 6 is a schematic view of the compression ring of the dynamic pressurized tissue engineering valve cell inoculator according to an embodiment of the present invention;
in the figure: 1. an upper base; 2. an upper cover; 3. a lower base; 4. a lower cover; 5. a valve clamp; 51. a sewing seat; 52. a fixing ring; 53. a support; 54. a growth chamber; 55. shaping balls; 6. an upper liquid chamber; 7. a lower liquid chamber; 8. an upper air chamber; 9. a lower air chamber; 10. an upper diaphragm; 11. a lower diaphragm; 12. buckling; 13. an upper liquid injection port; 14. an upper vent; 15. an upper gas injection port; 16. a lower liquid pouring port; 17. a lower exhaust port; 18. a lower air inlet; 19. a rubber seal ring; 20. a manometer; 21. a thread groove; 22. pressing a ring; 23. an external thread; 24. threaded plug
The specific implementation mode is as follows:
the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
As shown in fig. 1, 2, 3, 4, 5 and 6, the dynamic pressurized tissue engineering valve cell inoculator comprises an upper base 1, an upper cover 2, a lower base 3, a lower cover 4 and a valve clamp 5, wherein an upper liquid chamber 6 is arranged in the upper base 1, a lower liquid chamber 7 is arranged in the lower base 3, an upper air chamber 8 is arranged in the upper cover 2, a lower air chamber 9 is arranged in the lower cover 4, the upper cover 2 is connected with the upper base 1, an upper diaphragm 10 is arranged between the upper air chamber 8 and the upper liquid chamber 6, the lower cover 4 is connected with the lower base 3, a lower diaphragm 11 is arranged between the lower air chamber 9 and the lower liquid chamber 7, the valve clamp 5 is arranged in the upper liquid chamber 6, the upper base 1 and the lower base 3 are connected through a buckle 12, the upper liquid chamber 6 is communicated with the lower liquid chamber 7, an upper liquid injection port 13 and an upper exhaust port 14 which are communicated with the upper liquid chamber 6 are arranged on the upper base 1, an upper gas injection port 15, the lower base 3 is provided with a lower injection port 16 and a lower exhaust port 17 which are communicated with the lower liquid chamber 7, and the lower cover 4 is provided with a lower injection port 18 which is communicated with the lower air chamber 9.
Preferably, the valve clamp 5 comprises a sewing seat 51, a fixing ring 52 and a support 53, the fixing ring 52 is arranged between the sewing seat 51 and the support 53, a growth cavity 54 is formed between the support 53 and the fixing ring 52, a shaping ball 55 is arranged in the growth cavity 54, the sewing seat 51 is used for sewing the edge of the heart valve and placing the heart valve into the growth cavity 54, and the shaping ball 55 is used for pressing the heart valve on the support 53 to attach the heart valve to the support 53, so as to facilitate subsequent tissue culture.
Preferably, a rubber seal 19 is provided on the joint surface of the upper base 1 and the lower base 3 to seal the joint surface of the upper base 1 and the lower base 3 and prevent the cell suspension from leaking.
Preferably, the upper diaphragm 10 and the lower diaphragm 11 are both convex diaphragms and protrude in the direction of the upper liquid chamber 6 and the lower liquid chamber 7, respectively, so as to ensure the deformation direction of the upper diaphragm 10 and the lower diaphragm 11 when the upper air chamber 8 and the lower air chamber 9 are inflated and pressurized.
Preferably, the upper cover 2 and the lower cover 4 are respectively provided with a pressure gauge 20 communicated with the upper air chamber 8 and the lower air chamber 9, and the pressure gauges 20 are used for measuring the inflation pressure in the upper air chamber 8 and the lower air chamber 9 so as to control the inflation pressurization pressure.
Preferably, a thread groove 21 is formed in the upper liquid chamber 6, a pressing ring 22 is formed in the thread groove 21, an external thread 23 matched with the thread groove 21 is formed on the outer side of the pressing ring 22, and the valve clamp 5 is fixed in the upper liquid chamber 6 through the pressing ring 22, so that the valve clamp 5 can be conveniently taken out of the upper liquid chamber 6 while the fixing effect of the valve clamp 5 is guaranteed.
Preferably, the upper air injection port 15, the upper liquid injection port 13, the upper air exhaust port 14, the lower air injection port 18, the lower liquid injection port 16 and the lower air exhaust port 17 are respectively provided with a detachable threaded plug 24, so that plugging is facilitated.
Preferably, the upper diaphragm 10 and the lower diaphragm 11 are made of high-elasticity silica gel, so that the high-elasticity silica gel has sufficient elasticity, good corrosion resistance, is not easy to age, and is beneficial to long-term use.
The utility model discloses a method for using dynamic pressure tissue engineering valve cell inoculator is:
sewing three heart valves on a sewing seat 51 of a valve clamp 5 into a circular surface, and placing the circular surface into a growth cavity 54 to ensure that the heart valves are pressed and fixed on a support 53 by a shaping ball 55; the valve clamp 5 is put into the thread groove 21 of the upper liquid chamber 6, and the pressing ring 22 is screwed into the thread groove 21, so that the valve clamp 5 is fixed; connecting an upper base 1 and a lower base 3 through a buckle 12, arranging a rubber sealing ring 19 on the joint surface of the upper base 1 and the lower base 3, and sealing the joint surface of the upper base 1 and the lower base 3; injecting a cell suspension with a cell density of 100w/mL from the lower injection port 16 of the lower liquid chamber 7 while opening the lower vent 17 of the lower liquid chamber 7, stopping injection when the liquid level rises to be close to the lower vent 17 of the lower liquid chamber 7, and simultaneously closing the lower injection port 16 and the lower vent 17 of the lower liquid chamber 7; injecting a cell suspension with a cell density of 100w/mL from the upper injection port 13 of the upper liquid chamber 6, simultaneously opening the upper vent 14 of the upper liquid chamber 6, stopping injection when the liquid level rises to be close to the upper vent 14 of the upper liquid chamber 6, and simultaneously closing the upper injection port 13 and the upper vent 14 of the upper liquid chamber 6; adjusting the position of the cell inoculator, and discharging air while injecting liquid until the air in the upper liquid chamber 6 and the lower liquid chamber 7 is completely discharged; connecting an upper gas injection port 15 of the upper gas chamber 8 with an air pump, pressurizing the upper gas chamber 8 through the air pump, measuring the pressure through a pressure gauge 20, and closing the upper gas injection port 15 when the pressure value reaches a required pressure value (80-120 mmHg); connecting a lower air inlet 18 of the lower air chamber 9 with an air pump, pressurizing the lower air chamber 9 by the air pump, measuring the pressure by a pressure gauge 20, and closing the lower air inlet 18 when the pressure value reaches a required pressure value (80-120 mmHg); fixing a cell inoculator on a vertical rotary shaking table, setting the rotation speed of the shaking table to be 1rpm, putting the shaking table into a cell incubator, and dynamically pressurizing and inoculating for 12-24 hours; after inoculation is finished, the upper air injection port 15 and the lower air injection port 18 are opened to deflate and reduce pressure, cell suspensions in the upper liquid chamber 6 and the lower liquid chamber 7 are sucked out, the buckle 12 is loosened, the valve is taken out, and the valve is put on the dynamic bioreactor to continue dynamic culture.
To sum up, the utility model provides a developments pressurization tissue engineering valve cell inoculator for human heart tissue engineering valve cell inoculation, among the solution prior art, the tissue engineering valve cell that carries out the external culture is loose with being connected of support, and the cell can be washed away by the body fluid circulation in the human body easily after implanting the human body, does not play the problem that should have the effect.
The present invention has been described above by way of example, but the present invention is not limited to the above-mentioned embodiments, and any modification or variation based on the present invention is within the scope of the present invention.
Claims (8)
1. A dynamic pressure tissue engineering valve cell inoculator, characterized in that: the valve clamp is arranged in the upper liquid chamber, the upper base is connected with the lower base through a buckle, the upper liquid chamber is communicated with the lower liquid chamber, an upper liquid injection port and an upper gas injection port which are communicated with the upper liquid chamber are arranged on the upper base, and a lower liquid injection port and a lower gas injection port which are communicated with the lower liquid chamber are arranged on the lower base, and a lower air inlet communicated with the lower air chamber is arranged on the lower cover.
2. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: the valve clamp comprises a sewing seat, a fixing ring and a support, wherein the fixing ring is arranged between the sewing seat and the support, a growth cavity is formed between the support and the fixing ring, and a shaping ball is arranged in the growth cavity.
3. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: and a rubber sealing ring is arranged on the joint surface of the upper base and the lower base.
4. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: the upper diaphragm and the lower diaphragm are both convex diaphragms that project in the direction of the upper liquid chamber and the lower liquid chamber, respectively.
5. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: and the upper cover and the lower cover are respectively provided with a pressure gauge communicated with the upper air chamber and the lower air chamber.
6. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: be equipped with the thread groove in the upper portion liquid chamber, be equipped with the clamping ring in the thread groove, the outside of clamping ring be equipped with thread groove complex external screw thread, the valve anchor clamps quilt the clamping ring is fixed in the upper portion liquid chamber.
7. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: the upper air injection port, the upper liquid injection port, the upper air exhaust port, the lower air injection port, the lower liquid injection port and the lower air exhaust port are all provided with detachable threaded plugs.
8. The dynamic pressurized tissue engineered valve cell inoculator of claim 1, wherein: the upper diaphragm and the lower diaphragm are made of high-elasticity silica gel.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021940357.5U CN212316141U (en) | 2020-09-08 | 2020-09-08 | Dynamic pressure tissue engineering valve cell inoculator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021940357.5U CN212316141U (en) | 2020-09-08 | 2020-09-08 | Dynamic pressure tissue engineering valve cell inoculator |
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| CN212316141U true CN212316141U (en) | 2021-01-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925939A (en) * | 2020-09-08 | 2020-11-13 | 华中科技大学同济医学院附属协和医院 | Dynamic pressurized tissue engineering valve cell inoculator and use method thereof |
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2020
- 2020-09-08 CN CN202021940357.5U patent/CN212316141U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925939A (en) * | 2020-09-08 | 2020-11-13 | 华中科技大学同济医学院附属协和医院 | Dynamic pressurized tissue engineering valve cell inoculator and use method thereof |
| CN111925939B (en) * | 2020-09-08 | 2023-07-04 | 华中科技大学同济医学院附属协和医院 | Application method of dynamic pressurization tissue engineering valve cell inoculator |
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