CN201244101Y - Pulsating movement bioreactor - Google Patents
Pulsating movement bioreactor Download PDFInfo
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- CN201244101Y CN201244101Y CNU200820029546XU CN200820029546U CN201244101Y CN 201244101 Y CN201244101 Y CN 201244101Y CN U200820029546X U CNU200820029546X U CN U200820029546XU CN 200820029546 U CN200820029546 U CN 200820029546U CN 201244101 Y CN201244101 Y CN 201244101Y
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- valve
- culture chamber
- artificial heart
- auxiliary device
- cavity
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Abstract
The utility model relates to a pulse bioreactor. The positive pressure system is opened to generate positive pressure to extrude the culture solution through an auxiliary artificial heart device during contraction, and the negative pressure system is opened to generate negative pressure, so that the culture solution flows back to the auxiliary artificial heart device during relaxation to form a pulsatile liquid, the mechanical stability is good and the operation is reliable. The utility model comprises a pulse bioreactor power device, wherein the pulse bioreactor power device is connected with the auxiliary artificial heart device connected with a valve culture cavity, and the valve culture cavity is a liquid storage tank connected with the auxiliary artificial heart device.
Description
One, technical field:
This utility model relates to a kind of medical treatment and experiment apparatus, especially relates to a kind of impulsive motion bioreactor.
Two, background technology:
In the background technology, valvular heart disease is the important diseases of harm humans health, and artificial Cardiac Valve Replacement is the critical treatment method of valvular heart disease.The artificial heart valve that uses at present mainly contains mechanical prosthetic valve and bioprosthetic valve clinically, but its shortcoming is separately all arranged, and there are problems such as hemorrhage, the thrombosis of wanting lifelong anticoagulant, potentiality, thromboembolism in mechanical prosthetic valve; Then easily calcification of bioprosthetic valve, degeneration consequently decay and lose function.
From nineteen ninety-five, Shinoka plants sheep aorta wall fibroblast and vascular endothelial cell successively on polyglycolic acid (PGA) support in synthetic and makes valve, implants Pulmonis caprae seu ovis arterial valve district, obtains initial success.Thereby allow people see the hope of TEHV.After this Sodian and Hoerstrup etc. make up the organizational project valve with artificial synthesized degradable material PGA, PHA and P4HB etc. as timbering material.O ' Brien etc. should spend natural material research organization engineering valves such as cell porcine aortic valve.Proved that it makes up the feasibility of TEHV.
But, the organizational project valve of all these external static construction, cell is when external static culture, can lose differentiation function, the cell adhesion forces of In vitro culture is low, physiological function is compared obvious deficiency with active somatic cell, the requirement that the mechanical property of the TEHV opposing blood stream pressure of structure does not reach normal aortic valve far away.After implanting, the cell of valve can come off with the impact of blood flow in the plantation.Therefore, TEHV to external structure carries out the common recognition that " external preset " becomes people, the utilization impulsive motion bioreactor is at the hydrodynamics environment of in-vitro simulated intracorporeal heart valve, make the TEHV of static construction carry out external pre-adaptation, external structure become to resist blood flow stress, biomaterial degraded, can self-reconstruct the TEHV of telotism.
Three, utility model content:
The purpose of this utility model is to provide a kind of impulsive motion bioreactor, the open malleation that produces of its positive pressure system when shrinking extrudes the artificial heart auxiliary device with culture fluid, the open negative pressure that produces of negative pressure system makes culture fluid pass back into the artificial heart auxiliary device when diastole, form pulsation liquid, and good mechanical stability, reliable.
For achieving the above object, the technical solution adopted in the utility model is:
A kind of impulsive motion bioreactor, it is characterized in that: comprise the impulsive motion bioreactor power set, the impulsive motion bioreactor power set are connected with ventricular assist device, and ventricular assist device is connected with the valve culture chamber, the valve culture chamber is connected with fluid reservoir, and fluid reservoir is connected with ventricular assist device.
Above-mentioned pulsation reactor power set comprise air compressor machine and vacuum pump, air compressor machine, accumulator, pressure-regulating valve and high-frequency electromagnetic valve are connected successively by pipeline, vacuum pump, accumulator, pressure-regulating valve and high-frequency electromagnetic valve are connected successively, the pipeline of drawing from two high-frequency electromagnetic valves merges into a pipeline connection artificial heart auxiliary device, connect manograph on the pipeline that connects the artificial heart auxiliary device, master control system connects control parameter button, high-frequency electromagnetic valve, manograph and display screen respectively.
Above-mentioned valve culture chamber comprises the culture chamber cavity, and cavity is connected and composed by left and right sides two parts, and the junction is provided with the valve holder, and the cavity two ends are respectively inlet and liquid outlet; The valve holder adopts stainless steel substrates to make, and the valve holder is the circulus of hollow, is having equidistant aperture near the inner edge place, and the internal diameter of valve holder is 17mm, 19mm, 21mm, 23mmmm or 25mm; The culture chamber cavity adopts makrolon material to make, and the internal diameter of inlet and liquid outlet is 10mm, and the internal diameter of cavity is 27mm; Having internal diameter on the culture chamber cavity is the side mouth of 4mm, and the side mouth is connected with pressure transducer by pipeline.
Compared with prior art, the advantage and the effect that the utlity model has are as follows:
This utility model impulsive motion bioreactor is used for the structure of TEHV.This utility model is made up of pulsation reactor power set, artificial heart auxiliary device, valve culture chamber, liquid containing bottle and connecting pipe.The key link that pulsation reactor power set make up is two pumps---pneumatics pump and vacuum pump, makes malleation and negative pressure respectively.The open malleation that produces of positive pressure system extrudes the artificial heart auxiliary device with culture fluid when shrinking, and the open negative pressure that produces of negative pressure system makes culture fluid pass back into the artificial heart auxiliary device when diastole, forms pulsation liquid.Whole power set can be realized malleation 250mmHg under immunization with gD DNA vaccine, negative pressure-100mmHg, frequency 10-90 time/minute.Basically can satisfy the demand.
Four, description of drawings:
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is the structural representation of pulsation reactor power set in this utility model;
Fig. 3 is the program flow diagram of pulsation reactor power set master control system in this utility model;
Fig. 4 is valve culture chamber center sagittate section figure in this utility model;
Fig. 5 is a valve culture chamber center cross-sectional view in this utility model;
Fig. 6 is the structural representation of valve holder in this utility model.
Among the figure, 1-pulsation reactor power set, 2-artificial heart auxiliary device, 3-valve culture chamber, 4-fluid reservoir, 5-master control system, the 6-display screen, the control of 7-power supply, 8-air compressor machine, 9-control parameter panel, 10-high-frequency electromagnetic valve, 11-manograph, the 12-conduit, 13-accumulator, 14-pressure-regulating valve, 15-artificial heart auxiliary device, 16-conduit, 17-accumulator, the 18-pressure-regulating valve, 19-high-frequency electromagnetic valve, 20-conduit, the 21-vacuum pump, 22-valve culture chamber left-half, 23-valve culture chamber right half part, the 24-screw, 25-side mouth, 26-valve holder, the 27-aperture, the 28-screw socket.
Five, the specific embodiment:
Referring to Fig. 1, whole impulsive motion bioreactor is made up of two parts: the A part: be the part on the dotted line left side, be pulsation reactor power set 1, it can provide round pulse pneumatic.The B part: the part for dotted line the right mainly is made of artificial heart auxiliary device 2, valve culture chamber 3 and fluid reservoir 4.Referring to Fig. 1, impulsive motion bioreactor power set 1 are connected with artificial heart auxiliary device 2 that (artificial heart auxiliary 2 is existing product, not the application's inventive point), artificial heart auxiliary device 2 is connected with valve culture chamber 3, valve culture chamber 3 is connected with fluid reservoir 4, fluid reservoir 4 is connected with artificial heart auxiliary device 2, is formed by connecting by the medical PVC pipe between each device.During real work, power set are after producing pulse pneumatic, and the barrier film that promotes in the artificial heart auxiliary device 2 is done back and forth movement, will beat then and pass to the liquid part, artificial heart auxiliary device 2 has the opposite mechanical valve prosthesis of both direction, can guarantee the one-way flow of liquid.When air pump was discharged gas, liquid flowed out artificial heart auxiliary device 2, flows to valve culture chamber 3 and fluid reservoir 4.When air pump resorption gas, liquid passes back into artificial heart auxiliary device 2 by fluid reservoir 4.
Referring to Fig. 2, this utility model comprises air compressor machine 8 and vacuum pump 21, air compressor machine 8, accumulator 13, pressure-regulating valve 14 and high-frequency electromagnetic valve 10 are connected successively by pipeline, vacuum pump 21, accumulator 17, pressure-regulating valve 18 and high-frequency electromagnetic valve 19 are connected successively, and the pipeline of drawing from two high-frequency electromagnetic valves merges into a pipeline connection artificial heart auxiliary device 15 then.On the pipeline that connects artificial heart auxiliary device 15, connect manograph 11.Master control system 5 connects control parameter button, high-frequency electromagnetic valve, manograph 11 and display screen 6 etc.Power supply connects air compressor machine 8, vacuum pump 21, master control system 5, display screen 6, control parameter panel 9, manograph 11 and high-frequency electromagnetic valve to supply with electric energy.Power set need adopt less and the electromagnetic pump that can long time continuous working of noise.During real work, after powering on, air compressor machine 8 and vacuum pump 21 all commence work without delay, and make the accumulator that is attached thereto reach required pressure within a short period of time.At this moment, import the time span of systole and relaxing period respectively by prompting by peripheral hardware.CPU alternately starts and these two high-frequency electromagnetic valves that accumulator links to each other according to the internal timing device subsequently, and the pressure of correspondence is reached in the ventricle of artificial heart.At this moment CPU detects artificial heart systole and relaxing period pressure separately by high-precision pressure transducer again, and shows.The operator can be at this moment comes the corresponding pressure of manual adjustments by adjusting respectively with systolic pressure and the corresponding pressure-regulating valve of diastolic pressure.
Referring to Fig. 3, master control system realizes that based on Single Chip Microcomputer (SCM) system its main ingredient has: high stability single-chip microcomputer, 16 key boards, 128*64 dot matrix LCD display, 24 high-precision gas pressure harvesters and high-frequency electromagnetic valve etc.Wherein, the gas pressure intensity harvester is used for the measurement to the artificial heart air chamber pressure, and electromagnetic valve is used for controlling the air pump that is connected with air chamber at the cardiac cycle internal recycle.The work process of system is by the program decision that writes in the single-chip microcomputer, and the flow chart of program as shown in Figure 3.
Referring to Fig. 4 and Fig. 5, whole culture chamber is made up of left and right sides two parts in this utility model, be valve culture chamber left-half 22 and valve culture chamber right half part 23, both ends open internal diameter 10mm, be easy to connecting pipe, and the chamber of middle arrangement valve is that the size design of having considered Cardiac valve prosthesis forms, and internal diameter 27mm can satisfy inserting of different big or small valves.Couple together by CARBURIZING FURNACE FOR STAINLESS FASTENER 24 between two parts, the middle thick groove of a 1mm that leaves is placed valve holder 26 after coupling together.In place near the right outlet, the side mouth 25 that to open an internal diameter be 4mm can be used for connecting pressure transducer, uses for pressure measurement, referring to Fig. 6, valve holder 26 is made by stainless steel substrates, is the circulus of a hollow, in the place near inner edge, make a call to 12 equidistant apertures 27, be used for making valve and use, the internal diameter of valve holder can be arranged to different sizes as 17,19,21,23 or 25mm etc., can be used for making the valves of different sizes.During actual the use, earlier valve is sewn on the valve holder 26, then valve holder 26 is placed between two parts of culture chamber cavity, again that two parts cavity is fixedly connected by CARBURIZING FURNACE FOR STAINLESS FASTENER 24.
This utility model fluid is cultivated part: in the middle of the artificial heart auxiliary device elastica, barrier film both sides being arranged is two chambers, and a side is an air cavity---connect pulsation reactor power set, a side is a liquid chambers---connect valve culture chamber and fluid reservoir.The artificial heart auxiliary device is imported and exported and to be respectively two opposite valves of direction, guarantees that liquid does one-way flow.The valve culture chamber adopts Merlon to constitute, and separated into two parts links together by screw, and middle elastic tape is involutory, good seal performance.Accompany a rustless steel lobe frame in the middle of two parts, all very convenient with its fixed biologically lobe, organizational project lobe leaf, fixed form is with Prolene line or polyester thread it to be sewn on the lobe frame to get final product.Lobe frame internal diameter is adjustable 17,19,21,23, the 25mm specification, can satisfy the fixed demand of each model valve.Liquid containing bottle is also formed by specifications design by Merlon, and inlet is last, the liquid outlet infra, and the about 1000ml of whole volume can finish the gas exchange of culture medium and incubator by the air inlet of top.The liquid containing bottle volume is too small, and culture medium is overflowed easily, the excessive culture medium of then wasting of volume.Whole cultivation part is all used ethylene oxide sterilizing.
In the performance test, the impulsive motion bioreactor good mechanical stability, reliable, there is not leakage at each position in conjunction with closely.In servicely can produce pulsation liquid, move 5 days continuously, during do not appear any fault.Thereafter, with of the running of LB culture fluid through 5 days.Light microscopic is no antibacterial and mould contamination down, and antibacterial and fungus growth are not all found in nutrient agar of the capable general antibacterial of LB culture fluid and the test of mold agar culture medium coated plate.Illustrate that impulsive motion bioreactor has good antifouling property.The cell toxicity test of this group material, the biocompatibility and the toxicity of coming evaluating material from cellular level.The result shows that Merlon and medical PVC in vitro toxicity are very low, and blank group does not have significant difference, and the toxicity of pair cell is little, illustrate that Merlon can be used as the material that makes up valve culture chamber and fluid reservoir, and medical PVC can be used as the connecting pipe use.
Hydrodynamics detects, and under the driving of pulsation reactor power set, this culture systems pressure can reach 0-120mmHg, and the about 50-3500ml/min of flow can reach the requirement of external pulsation pre-adaptation basically.Sodian35 etc. utilize Merlon, silica gel, pulsate pump to develop impulsive motion bioreactor, and this reactor can produce the flow of 100ml/min-3000ml/min, the pressure of 3mmHg-150mmHg.
Claims (3)
1, a kind of impulsive motion bioreactor, it is characterized in that: comprise impulsive motion bioreactor power set (1), impulsive motion bioreactor power set (1) are connected with artificial heart auxiliary device (2), artificial heart auxiliary device (2) is connected with valve culture chamber (3), valve culture chamber (3) is connected with fluid reservoir (4), and fluid reservoir (4) is connected with artificial heart auxiliary device (2).
2, impulsive motion bioreactor according to claim 1, it is characterized in that: pulsation reactor power set comprise air compressor machine (8) and vacuum pump (21), air compressor machine (8), accumulator (13), pressure-regulating valve (14) is connected by pipeline successively with high-frequency electromagnetic valve (10), vacuum pump (21), accumulator (17), pressure-regulating valve (18) is connected successively with high-frequency electromagnetic valve (19), the pipeline of drawing from two high-frequency electromagnetic valves merges into a pipeline connection artificial heart auxiliary device (15), connect manograph (11) on the pipeline that connects artificial heart auxiliary device (15), master control system (5) connects control parameter button respectively, high-frequency electromagnetic valve, manograph (11) and display screen (6).
3, impulsive motion bioreactor according to claim 1, it is characterized in that: the valve culture chamber comprises the culture chamber cavity, cavity is connected and composed by valve culture chamber left-half (22) and valve culture chamber right half part (23) two parts, the junction is provided with valve holder (26), and the cavity two ends are respectively inlet and liquid outlet; Valve holder (26) adopts stainless steel substrates to make, and valve holder (26) is the circulus of hollow, is having equidistant aperture (27) near the inner edge place, and the internal diameter of valve holder (26) is 17mm, 19mm, 21mm, 23mmmm or 25mm; The culture chamber cavity adopts makrolon material to make, and the internal diameter of inlet and liquid outlet is 10mm, and the internal diameter of cavity is 27mm; Having internal diameter on the culture chamber cavity is the side mouth (25) of 4mm, and side mouth (25) is connected with pressure transducer by pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200820029546XU CN201244101Y (en) | 2008-07-03 | 2008-07-03 | Pulsating movement bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200820029546XU CN201244101Y (en) | 2008-07-03 | 2008-07-03 | Pulsating movement bioreactor |
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| CN201244101Y true CN201244101Y (en) | 2009-05-27 |
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| CNU200820029546XU Expired - Fee Related CN201244101Y (en) | 2008-07-03 | 2008-07-03 | Pulsating movement bioreactor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013060067A1 (en) * | 2011-10-27 | 2013-05-02 | 上海微创医疗器械（集团）有限公司 | Method and device for fixing artificial bioprosthetic valve by membrane pervaporation |
| CN105255730A (en) * | 2015-10-21 | 2016-01-20 | 陕西科技大学 | Three-dimensional blood vessel fixing device and using method thereof |
| CN107177500A (en) * | 2017-05-24 | 2017-09-19 | 广东省人民医院(广东省医学科学院) | A kind of bionical impulsive motion bioreactor based on Luo Ye pumps |
| CN109125828A (en) * | 2018-06-04 | 2019-01-04 | 林青 | A kind of artificial heart control equipment and application method |
-
2008
- 2008-07-03 CN CNU200820029546XU patent/CN201244101Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013060067A1 (en) * | 2011-10-27 | 2013-05-02 | 上海微创医疗器械（集团）有限公司 | Method and device for fixing artificial bioprosthetic valve by membrane pervaporation |
| CN105255730A (en) * | 2015-10-21 | 2016-01-20 | 陕西科技大学 | Three-dimensional blood vessel fixing device and using method thereof |
| CN107177500A (en) * | 2017-05-24 | 2017-09-19 | 广东省人民医院(广东省医学科学院) | A kind of bionical impulsive motion bioreactor based on Luo Ye pumps |
| CN109125828A (en) * | 2018-06-04 | 2019-01-04 | 林青 | A kind of artificial heart control equipment and application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090527 Termination date: 20100703 |