CN212246993U - Device for rapidly extracting DNA (deoxyribonucleic acid) in animal tissue by virtue of paramagnetic particle method - Google Patents
Device for rapidly extracting DNA (deoxyribonucleic acid) in animal tissue by virtue of paramagnetic particle method Download PDFInfo
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- CN212246993U CN212246993U CN202020582041.7U CN202020582041U CN212246993U CN 212246993 U CN212246993 U CN 212246993U CN 202020582041 U CN202020582041 U CN 202020582041U CN 212246993 U CN212246993 U CN 212246993U
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Abstract
The utility model discloses a device for rapidly extracting DNA in animal tissues by a magnetic bead method, which comprises a box body; the interior of the box body is of a hollow structure, the top of the box body is hinged with a cover plate, and a handle is fixedly arranged on the cover plate; the bottom of the box body is rotatably provided with a turntable, and the left side and the right side of the turntable are symmetrically and fixedly provided with a centrifugal machine and a magnetic frame; the upper portion of box is installed and is driven the adjustment mechanism that the test tube removed, and adjustment mechanism includes the crane and carries out the fixed establishment to the test tube, and fixed establishment fixed mounting is on the crane. The utility model discloses an it installs the carousel to rotate in the bottom of box, and the left and right sides symmetry fixed mounting of carousel has centrifuge and magnetic force frame, and the test tube that will be equipped with the solution of animal cell puts into again after centrifuge centrifugal treatment and puts into the magnetic force frame, carries out the magnetic bead separation, draws the DNA in the animal tissue, simplifies the operating procedure that DNA drawed, improves DNA extraction rate.
Description
Technical Field
The utility model relates to a DNA draws technical field, specifically is a device of DNA among magnetic bead method rapid extraction animal tissue.
Background
In order to ensure efficient extraction of DNA and that the extracted DNA can be used in the relevant work, the extraction of DNA must follow the following principles: 1) the primary structure of the DNA must be complete; 2) the DNA sample should not contain organic solvent for inhibiting enzyme and metal ions with too high concentration; 3) controlling the concentration of other biomacromolecules to avoid polluting a DNA sample; 4) removing nucleic acids other than DNA.
Extraction of DNA currently, methods for DNA extraction are mainly: the phenol extraction method comprises the steps of crushing cells by using protease and SDS (sodium dodecyl sulfate), digesting proteins in the cells to expose DNA, and extracting by using phenol-chloroform, but digesting the proteins and repeatedly extracting the DNA, so that the steps are complicated, certain influence is caused on the DNA, and the quality of the extracted DNA is low; the formamide depolymerization method is similar to the phenol extraction method, and is different in that after cells are broken, firstly, the combined protein and DNA are depolymerized by high-concentration formamide and then dialyzed to obtain DNA, the quality of the DNA obtained by the method is slightly higher than that of the DNA obtained by the phenol extraction method, the operation is simpler than that of the phenol extraction method, but the application requirement of a DNA sample is still difficult to meet, and the quality of the DNA needs to be further improved; the method for preparing the DNA rapidly by alkali denaturation comprises the steps of treating the DNA in an alkali environment for 20min by NaOH, neutralizing the DNA by hydrochloric acid, centrifuging the DNA, and taking supernatant to obtain the DNA.
Therefore, in view of the above situation, there is an urgent need to develop a device for rapidly extracting DNA from animal tissues by using the magnetic bead method, so as to overcome the shortcomings in the current practical application.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a device of DNA among magnetic bead method draws animal tissue fast to solve the problem that proposes in the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
a device for rapidly extracting DNA from animal tissues by a paramagnetic particle method comprises a box body; the interior of the box body is of a hollow structure, the top of the box body is hinged with a cover plate, and a handle is fixedly arranged on the cover plate; the bottom of the box body is rotatably provided with a turntable, and the left side and the right side of the turntable are symmetrically and fixedly provided with a centrifugal machine and a magnetic frame; the upper portion of box is installed and is driven the adjustment mechanism that the test tube removed, and adjustment mechanism includes the crane and carries out the fixed establishment to the test tube, and fixed establishment fixed mounting is on the crane.
As a further aspect of the present invention: the rotary table is rotatably installed at the bottom of the box body through a rotating shaft, a driving motor is fixedly installed at the bottom of the box body, and the output end of the driving motor is in transmission connection with the rotating shaft through a bevel gear set.
As a further aspect of the present invention: the left side and the right side inside the box body are symmetrically and rotatably provided with two screw rods, the screw rods are in threaded connection with screw sleeves, one sides of the screw sleeves are in sliding connection with the inner wall of the box body, and the other sides of the screw sleeves are fixedly connected with the lifting frame.
As a further aspect of the present invention: the upper ends of the two screw rods are fixedly provided with belt pulleys, and the two belt pulleys are in transmission connection through a belt; and the box body is also internally provided with a rotating motor for driving one of the screw rods to rotate.
As a further aspect of the present invention: the cover plate is embedded with a liquid inlet hopper which is positioned right above the fixing mechanism.
As a further aspect of the present invention: the fixing mechanism comprises a clamping ring, a plurality of sliding grooves are uniformly formed in the clamping ring, an electromagnet is fixedly mounted at the bottom of each sliding groove, a sliding rod is slidably mounted in each sliding groove, one end of each sliding rod is fixedly connected with the electromagnet through a spring, and the other end of each sliding rod extends out of the corresponding sliding groove and is fixedly connected with the corresponding clamping piece.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses an it installs the carousel to rotate in the bottom of box, and the left and right sides symmetry fixed mounting of carousel has centrifuge and magnetic force frame, and the test tube that will be equipped with the solution of animal cell puts into again after centrifuge centrifugal treatment and puts into the magnetic force frame, carries out the magnetic bead separation, draws the DNA in the animal tissue, simplifies the operating procedure that DNA drawed, improves DNA extraction rate.
Drawings
FIG. 1 is a schematic structural diagram of a device for rapidly extracting DNA from animal tissues by a magnetic bead method.
FIG. 2 is a schematic view of a liquid inlet funnel in a device for rapidly extracting DNA from animal tissues by a magnetic bead method.
FIG. 3 is a schematic structural diagram of a fixing mechanism in a device for rapidly extracting DNA from animal tissues by a magnetic bead method.
In the figure: 1-box body, 2-screw rod, 3-screw sleeve, 4-belt, 5-cover plate, 6-liquid inlet bucket, 7-handle, 8-operation panel, 9-lifting frame, 10-fixing mechanism, 11-test tube, 12-centrifuge, 13-rotary table, 14-magnetic frame, 15-bevel gear set, 16-driving motor, 17-clamping ring, 18-electromagnet, 19-spring, 20-slide bar and 21-clamping piece.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Example 1
Referring to fig. 1-2, in an embodiment of the present invention, an apparatus for rapidly extracting DNA from animal tissue by using a magnetic bead method includes a box 1; the box body 1 is of a hollow structure, a cover plate 5 is hinged to the top of the box body 1, and a handle 7 is fixedly mounted on the cover plate 5 so as to facilitate the opening and closing of the cover plate 5; a rotating disc 13 is rotatably arranged at the bottom of the box body 1, a centrifuge 12 and a magnetic frame 14 are symmetrically and fixedly arranged at the left side and the right side of the rotating disc 13, a test tube filled with a solution of animal cells is placed in the centrifuge 12 for centrifugal treatment and then placed in the magnetic frame 14 for magnetic bead separation, and DNA in animal tissues is extracted;
specifically, in this embodiment, the rotating disc 13 is rotatably mounted at the bottom of the box body 1 through a rotating shaft, the bottom of the box body 1 is further fixedly mounted with a driving motor 16, and an output end of the driving motor 16 is in transmission connection with the rotating shaft through a bevel gear set 15, and is used for driving the rotating shaft and the rotating disc 13 to rotate;
the upper part of the box body 1 is provided with an adjusting mechanism for driving the test tube 11 to move, the adjusting mechanism comprises a lifting frame 9 and a fixing mechanism 10 for fixing the test tube 11, the fixing mechanism 10 is fixedly arranged on the lifting frame 9, the lifting frame 9 is driven to slide up and down in the box body 1 to drive the test tube 11 to move, and the test tube is placed in a centrifuge 12 or a magnetic frame 14;
two screw rods 2 are symmetrically and rotatably arranged on the left side and the right side inside the box body 1, screw sleeves 3 are connected to the screw rods 2 in a threaded mode, one sides of the screw sleeves 3 are connected with the inner wall of the box body 1 in a sliding mode, and the other sides of the screw sleeves 3 are fixedly connected with the lifting frame 9;
the upper ends of the two screw rods 2 are fixedly provided with belt pulleys, and the two belt pulleys are in transmission connection through a belt 4 and are used for driving the two screw rods 2 to simultaneously rotate in the same direction; a rotating motor (not shown in the figure) for driving one screw rod 2 to rotate is further arranged in the box body 1;
inlay on the apron 5 and install into liquid fill 6, the liquid fill 6 is located fixed establishment 10 directly over, through advance liquid fill 6 to the test tube on the fixed establishment 10 instil into animal tissue or carry out the supernatant and draw, also can open apron 5 and carry out instil into and the supernatant of animal tissue and draw.
Example 2
Referring to fig. 1-2, in an embodiment of the present invention, an apparatus for rapidly extracting DNA from animal tissue by using a magnetic bead method includes a box 1; the box body 1 is of a hollow structure, a cover plate 5 is hinged to the top of the box body 1, and a handle 7 is fixedly mounted on the cover plate 5 so as to facilitate the opening and closing of the cover plate 5; a rotating disc 13 is rotatably arranged at the bottom of the box body 1, a centrifuge 12 and a magnetic frame 14 are symmetrically and fixedly arranged at the left side and the right side of the rotating disc 13, a test tube filled with a solution of animal cells is placed in the centrifuge 12 for centrifugal treatment and then placed in the magnetic frame 14 for magnetic bead separation, and DNA in animal tissues is extracted;
specifically, in this embodiment, the rotating disc 13 is rotatably mounted at the bottom of the box body 1 through a rotating shaft, the bottom of the box body 1 is further fixedly mounted with a driving motor 16, and an output end of the driving motor 16 is in transmission connection with the rotating shaft through a bevel gear set 15, and is used for driving the rotating shaft and the rotating disc 13 to rotate;
the upper part of the box body 1 is provided with an adjusting mechanism for driving the test tube 11 to move, the adjusting mechanism comprises a lifting frame 9 and a fixing mechanism 10 for fixing the test tube 11, the fixing mechanism 10 is fixedly arranged on the lifting frame 9, the lifting frame 9 is driven to slide up and down in the box body 1 to drive the test tube 11 to move, and the test tube is placed in a centrifuge 12 or a magnetic frame 14;
two screw rods 2 are symmetrically and rotatably arranged on the left side and the right side inside the box body 1, screw sleeves 3 are connected to the screw rods 2 in a threaded mode, one sides of the screw sleeves 3 are connected with the inner wall of the box body 1 in a sliding mode, and the other sides of the screw sleeves 3 are fixedly connected with the lifting frame 9;
the upper ends of the two screw rods 2 are fixedly provided with belt pulleys, and the two belt pulleys are in transmission connection through a belt 4 and are used for driving the two screw rods 2 to simultaneously rotate in the same direction; a rotating motor (not shown in the figure) for driving one screw rod 2 to rotate is further arranged in the box body 1;
inlay on the apron 5 and install into liquid fill 6, the liquid fill 6 is located fixed establishment 10 directly over, through advance liquid fill 6 to the test tube on the fixed establishment 10 instil into animal tissue or carry out the supernatant and draw, also can open apron 5 and carry out instil into and the supernatant of animal tissue and draw.
Referring to fig. 3, the difference between the present embodiment and embodiment 1 is:
The above is only the preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, without departing from the concept of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent.
Claims (6)
1. A device for rapidly extracting DNA in animal tissues by a magnetic bead method is characterized by comprising a box body (1); the box body (1) is internally of a hollow structure, the top of the box body (1) is hinged with a cover plate (5), and a handle (7) is fixedly arranged on the cover plate (5); a rotary table (13) is rotatably arranged at the bottom of the box body (1), and a centrifugal machine (12) and a magnetic frame (14) are symmetrically and fixedly arranged at the left side and the right side of the rotary table (13); the upper portion of box (1) is installed and is driven the adjustment mechanism that test tube (11) removed, and adjustment mechanism includes crane (9) and fixed establishment (10) that go on fixing test tube (11), and fixed establishment (10) fixed mounting is on crane (9).
2. The device for rapidly extracting DNA from animal tissues by the magnetic bead method according to claim 1, wherein the rotating disc (13) is rotatably installed at the bottom of the box body (1) through a rotating shaft, a driving motor (16) is fixedly installed at the bottom of the box body (1), and an output end of the driving motor (16) is in transmission connection with the rotating shaft through a bevel gear set (15).
3. The device for rapidly extracting DNA from animal tissues by the magnetic bead method according to claim 2, wherein two screws (2) are symmetrically and rotatably installed on the left side and the right side inside the box body (1), a threaded sleeve (3) is connected to the screws (2) in a threaded manner, one side of the threaded sleeve (3) is slidably connected with the inner wall of the box body (1), and the other side of the threaded sleeve (3) is fixedly connected with the lifting frame (9).
4. The device for rapidly extracting DNA from animal tissues by the paramagnetic particle method according to claim 3, wherein the upper ends of the two screws (2) are fixedly provided with belt pulleys, and the two belt pulleys are in transmission connection through a belt (4); and a rotating motor for driving one of the screw rods (2) to rotate is further installed in the box body (1).
5. The device for rapidly extracting DNA from animal tissues by the magnetic bead method according to claim 1, wherein the cover plate (5) is embedded with a liquid inlet hopper (6), and the liquid inlet hopper (6) is positioned right above the fixing mechanism (10).
6. The device for rapidly extracting DNA from animal tissues by the magnetic bead method according to any one of claims 1 to 5, wherein the fixing mechanism (10) comprises a clamping ring (17), a plurality of sliding grooves are uniformly formed in the clamping ring (17), an electromagnet (18) is fixedly installed at the bottom of each sliding groove, a sliding rod (20) is further slidably installed in each sliding groove, one end of each sliding rod (20) is fixedly connected with the electromagnet (18) through a spring (19), and the other end of each sliding rod (20) extends out of each sliding groove and is fixedly connected with a clamping piece (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020582041.7U CN212246993U (en) | 2020-04-19 | 2020-04-19 | Device for rapidly extracting DNA (deoxyribonucleic acid) in animal tissue by virtue of paramagnetic particle method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020582041.7U CN212246993U (en) | 2020-04-19 | 2020-04-19 | Device for rapidly extracting DNA (deoxyribonucleic acid) in animal tissue by virtue of paramagnetic particle method |
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| CN212246993U true CN212246993U (en) | 2020-12-29 |
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| CN202020582041.7U Active CN212246993U (en) | 2020-04-19 | 2020-04-19 | Device for rapidly extracting DNA (deoxyribonucleic acid) in animal tissue by virtue of paramagnetic particle method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112628357A (en) * | 2021-02-15 | 2021-04-09 | 杭州天微基因科技有限公司 | A gear box for nucleic acid extraction |
| CN113484197A (en) * | 2021-07-02 | 2021-10-08 | 北京雅森博科科学仪器有限公司 | Freezing point osmotic pressure tester |
| CN113713880A (en) * | 2021-09-13 | 2021-11-30 | 云南省肿瘤医院(昆明医科大学第三附属医院) | Test-tube rack for molecular biology clinical examination |
-
2020
- 2020-04-19 CN CN202020582041.7U patent/CN212246993U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112628357A (en) * | 2021-02-15 | 2021-04-09 | 杭州天微基因科技有限公司 | A gear box for nucleic acid extraction |
| CN113484197A (en) * | 2021-07-02 | 2021-10-08 | 北京雅森博科科学仪器有限公司 | Freezing point osmotic pressure tester |
| CN113484197B (en) * | 2021-07-02 | 2023-10-13 | 北京雅森博科科学仪器有限公司 | Freezing point osmotic pressure tester |
| CN113713880A (en) * | 2021-09-13 | 2021-11-30 | 云南省肿瘤医院(昆明医科大学第三附属医院) | Test-tube rack for molecular biology clinical examination |
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