CN212199260U

CN212199260U - Closed consumptive material and detection box

Info

Publication number: CN212199260U
Application number: CN202020175645.XU
Authority: CN
Inventors: 叶宝春; 黄飞; 王珺
Original assignee: Hangzhou Jieyi Biotechnology Co ltd
Current assignee: Hangzhou Jieyi Biotechnology Co ltd
Priority date: 2020-02-17
Filing date: 2020-02-17
Publication date: 2020-12-22
Anticipated expiration: 2030-02-17
Also published as: WO2021164088A1

Abstract

The utility model belongs to the technical field of the biological reaction consumptive material, concretely relates to closed consumptive material and detection box. The closed consumable comprises a reagent tube and a tube cover, wherein a partition is arranged in the reagent tube and divides an inner cavity of the reagent tube into a first chamber and a second chamber; when the reagent tube is in a free state, the liquid in the first chamber is blocked by the partition part and cannot flow to the second chamber; when the reagent tube is in an external force action state, the liquid in the first chamber swings out of the separation part under the action of the external force and enters the second chamber. The utility model discloses a closed consumptive material can realize closed operation, after first step reaction time satisfies promptly, mixes the reagent of second step and the reagent of first step through exogenic action, has avoided the defect that open operation arouses.

Description

Closed consumptive material and detection box

Technical Field

The utility model belongs to the technical field of the biological reaction consumptive material, concretely relates to closed consumptive material and detection box.

Background

In molecular experiments, sometimes two steps are required: adding a sample and an amplification reaction system in the first step of experiment, and reacting for a first preset time; followed by the addition of a second step reagent and re-reacting for a second predetermined period of time. Molecular experiments are characterized by a nucleic acid amplification process, i.e., a low initial amount of sample is amplified millions of times in an hour. Since a large amount of template is present after amplification, an aerosol containing nucleic acid in the air may be caused in the open operation, and thus, when the same experiment is performed again, false positive may be easily caused.

SUMMERY OF THE UTILITY MODEL

Based on the above-mentioned not enough that exists among the prior art, the utility model provides a closed consumptive material.

In order to achieve the purpose of the utility model, the utility model adopts the following technical scheme:

a closed consumable comprises a reagent tube and a tube cover, wherein a partition is arranged in the reagent tube and divides an inner cavity of the reagent tube into a first chamber and a second chamber; when the reagent tube is in a free state, the liquid in the first chamber is blocked by the partition part and cannot flow to the second chamber; when the reagent tube is in an external force action state, the liquid in the first chamber swings out of the separation part under the action of the external force and enters the second chamber.

Preferably, the separator is a silica gel piece or a porous medium material, and the silica gel piece is provided with a cross-shaped opening.

As a preferred scheme, the porous medium material is a sponge, or the silica gel piece is a silica gel sleeve, the cross-shaped opening is located at the closed end of the silica gel sleeve, and the outer wall of the silica gel sleeve is provided with a vent hole for communicating the first chamber with the second chamber.

Preferably, the first chamber and the second chamber are distributed along the axial direction of the reagent tube, and the first chamber is positioned above the second chamber.

Preferably, the reagent tube comprises a first tube body and a second tube body which are detachably and hermetically connected, the first chamber is located in the first tube body, and the second chamber is located in the second tube body.

Preferably, the first chamber and the second chamber are distributed along the radial direction of the reagent tube, and the volume of the first chamber is smaller than that of the second chamber; when the tube cover covers the reagent tube, a gap for liquid to move from the first chamber to the second chamber is formed between the top of the separating piece and the tube cover.

Preferably, the separating part is a partition plate, the radial section of the partition plate along the reagent tube is of an arc-shaped structure, and the arc center of the arc-shaped structure is located in the direction of the first chamber.

Preferably, the external force application state is a centrifugal state.

The utility model also provides a detection box, including box body, lid and a plurality of as above arbitrary scheme closed consumptive material, the reagent pipe array of closed consumptive material is integrated in the box body, and is integrated in the lid with the tube cap of reagent pipe one-to-one.

Preferably, the joint surface of the box body and the cover body is an inclined surface.

Compared with the prior art, the utility model, beneficial effect is:

the utility model discloses a closed consumptive material can realize closed operation, after first step reaction time satisfies promptly, mixes the reagent of second step and the reagent of first step through exogenic action (for example centrifugation), has avoided the defect that open operation arouses.

The utility model discloses a detect box can realize that the batchization detects, promotes detection efficiency.

Drawings

FIG. 1 is a schematic structural view of a closed consumable according to a first embodiment of the present invention;

FIG. 2 is a schematic structural diagram of the closed consumable according to another view angle of the first embodiment of the present invention;

FIG. 3 is a cross-sectional view of portion B-B of FIG. 2;

FIG. 4 is an enlarged view of section C of FIG. 3;

FIG. 5 is a schematic structural diagram of the closed consumable according to another view angle of the first embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a testing cassette according to a fourth embodiment of the present invention;

FIG. 7 is a schematic structural diagram of a testing cassette according to a fourth embodiment of the present invention from another viewing angle;

FIG. 8 is a schematic structural view of a cartridge according to a fourth embodiment of the present invention from another viewing angle;

FIG. 9 is a schematic structural view of a cartridge according to a fourth embodiment of the present invention from another viewing angle;

FIG. 10 is a schematic structural view of a cartridge according to a fourth embodiment of the present invention from another viewing angle;

fig. 11 is a schematic structural view of a detection box according to a fourth embodiment of the present invention in a closed state.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention, the following description will explain embodiments of the present invention with reference to the accompanying drawings. It is obvious that the drawings in the following description are only examples of the invention, and that for a person skilled in the art, other drawings and embodiments can be obtained from these drawings without inventive effort. In addition, directional terms referred to in the following examples, for example: up, down, left, right, front or rear, etc., are simply directions with reference to the drawings. Accordingly, the directional terminology is used for purposes of illustration and is in no way limiting.

The first embodiment is as follows:

as shown in fig. 1 and 2, the closed consumable of this embodiment, including reagent pipe 1 and tube cap 2, reagent pipe 1 and tube cap 2 are the PT material, and integrative moulding plastics form, and the top of reagent pipe 1 has the mouth of pipe, and the sealed lid of tube cap 2 fits the mouth of pipe of reagent pipe 1 for the inner chamber of reagent pipe is in the encapsulated situation.

As shown in FIG. 3, a partition member 3 is arranged in the reagent tube 1, and the partition member 3 divides the inner cavity of the reagent tube into a first chamber I and a second chamber II.

Specifically, as shown in fig. 3, the reagent tube 1 includes an upper tube 11 and a lower tube 12 which are detachably and hermetically connected, the first chamber I is located in the first tube 11, the second chamber II is located in the second tube 12, that is, the first chamber I and the second chamber II are distributed along the axial direction (i.e., vertical direction) of the reagent tube, and the first chamber I is located above the second chamber II. Wherein, as shown in fig. 4, detachable sealing connection between the upper tube body 11 and the lower tube body 12 is a buckle sealing structure E, namely, the inner peripheral wall of the bottom of the upper tube body 11 is provided with a slot, a plurality of clamping holes are distributed in the circumferential direction of the slot, the top of the lower tube body 12 is provided with an inserting ring in inserting connection with the slot, the outer peripheral wall of the inserting ring is provided with elastic buckles in one-to-one correspondence with the clamping holes, and the detachable sealing connection between the upper tube body and the lower tube body is realized through the clamping sealing cooperation of the elastic buckles and the clamping holes. In addition, the detachable sealing connection mode can also be a threaded connection mode or other existing clamping connection modes.

The separator 3 is a silica gel sleeve, the structure is a sleeve structure with a closed bottom and an open top, and can also be a wafer structure and the like, and the structure of the separator is not limited; the separating piece can be placed in the reagent tube, so that the outer wall of the separating piece is tightly matched with the inner wall of the reagent tube, and the separating piece can be integrally connected with the reagent tube through secondary injection molding; wherein, the bottom of separator 3 has cross opening 30, and cross opening 30 easily produces the deformation under the exogenic action to liquid in the first chamber gets into in the second chamber and mixes with the liquid in the second chamber. In addition, as shown in fig. 5, the outer wall of the partition 3 has three vertical vent holes D (the number is not limited to three) communicating the first chamber and the second chamber, and air of the upper and lower chambers is communicated to prevent the upper liquid from leaking to the lower chamber due to gas pressure when the tube cover is closed, wherein the upper liquid is stored in the inner cavity of the silica gel sleeve of the first chamber.

When the reagent tube is in a free state, the liquid in the first chamber is blocked by the partition part and cannot flow to the second chamber; when the reagent tube is in an external force action state, the liquid in the first chamber swings out of the separation part under the action of the external force and enters the second chamber.

Specifically, the external force action state of the present embodiment is centrifugal operation, the first chamber is used for placing the second-step reagent, and the second chamber is used for placing the first-step reagent; and (3) carrying out a warm bath reaction on the whole closed consumable, and mixing the second-step reagent and the first-step reagent by centrifugal operation after the first-step reagent reaction time is met. Further, the external force acting state may be an oscillating operation, a shaking operation, or the like.

This embodiment still provides a detection box, including the closed consumptive material of box body, lid and a plurality of this embodiment, the reagent tube array of closed consumptive material is integrated in the box body, and the tube cap with reagent pipe one-to-one is integrated in the lid, obtains for example quadruple formula detection box, six antithetical couplet formula detection box etc. and realizes the batchization and detects, and the structure that detects the box can refer to the structure that detects the box of following embodiment four, and it is unnecessary to describe here.

Example two:

the enclosed consumable of this embodiment is different from the first embodiment in that:

the upper pipe body and the lower pipe body of the embodiment are integrally formed, and other structures are the same as those of the first embodiment; the structure diversification of the closed type consumable is realized so as to meet the requirements of different application occasions.

Example three:

the separator of the embodiment is made of a porous medium material, and the porous medium material is made of porous materials such as sponge and activated carbon and can store the second-step reagent and prevent the second-step reagent from entering the second chamber; the structure diversification of the closed type consumable is realized so as to meet the requirements of different application occasions.

Example four:

as shown in fig. 6, the partition 300 of the present embodiment divides the inner cavity of the reagent tube 100 into a first chamber I0 and a second chamber II0, which are distributed along the radial direction of the reagent tube, wherein the volume of the first chamber I0 is smaller than the volume of the second chamber II 0; when the tube cover 200 is covered on the reagent tube, a gap is formed between the top of the partition and the tube cover for the liquid to move from the first chamber to the second chamber, so that the second-step reagent in the first chamber moves into the second chamber through the gap between the partition and the tube cover under the centrifugal operation.

Specifically, the partition 300 is a partition plate, the partition plate is of an arc-shaped structure along the radial section of the reagent tube, and the arc center of the arc-shaped structure is located in the direction where the first chamber is located, so that under the action of centrifugal force, the liquid in the first chamber can rise along the peripheral wall of the first chamber and move into the second chamber.

As shown in fig. 6-11, the utility model provides a detect box still, including the closed consumptive material of box body A0, lid B0 and this embodiment, the reagent pipe 100 array of closed consumptive material is integrated in box body A0, and the tube cap 200 with the reagent pipe one-to-one is integrated in lid B0, obtains the quadruple formula and detects the box, can also obtain six antithetical couplets formula according to the quantity design of closed consumptive material and detect the box, eight antithetical couplets formula and detect the box etc. realize the batchization and detect. In addition, as shown in fig. 8, the joint surface of the box body and the cover body is an inclined plane F, the inclination is preferably 15 ° (not limited to 15 °), and the first chamber is located at the higher side of the inclined plane, and the second chamber is located at the lower side of the inclined plane, which is more beneficial for the liquid in the first chamber to rise along the peripheral wall of the first chamber and move into the second chamber.

As shown in FIG. 10, the reagent tube 100 has a fluorescence detection orientation G, and the detection optical head is attached to the outer wall of the second chamber perpendicularly, i.e., attached to the fluorescence detection orientation G, and functions as fluorescence detection, PCR.

Example five:

the difference between the closed consumable and the detecting box of the embodiment and the fourth embodiment is that:

the separator also can vertical plane baffle, and the binding face of box body and lid also can the plane, realizes that the structure of closed consumptive material and detection box is diversified to satisfy the demand of different application occasions.

Other structures can refer to embodiment four.

The foregoing has been a detailed description of the preferred embodiments and principles of the present invention, and it will be apparent to those skilled in the art that variations may be made in the specific embodiments based on the concepts of the present invention, and such variations are considered as within the scope of the present invention.

Claims

1. A closed consumable is characterized by comprising a reagent tube and a tube cover, wherein a partition is arranged in the reagent tube and divides an inner cavity of the reagent tube into a first chamber and a second chamber; when the reagent tube is in a free state, the liquid in the first chamber is blocked by the partition part and cannot flow to the second chamber; when the reagent tube is in an external force action state, the liquid in the first chamber swings out of the separation part under the action of the external force and enters the second chamber.

2. A closed consumable according to claim 1, in which the separator is a silicone or porous dielectric material, the silicone having a cross-shaped opening.

3. A closed consumable according to claim 2, in which the porous media material is a sponge; or, the silica gel spare is the silica gel sleeve pipe, and the cross opening is located the blind end of silica gel sleeve pipe, and the outer wall of silica gel sleeve pipe has the air vent of first cavity of intercommunication and second cavity.

4. A closed consumable according to claim 2, in which the first and second chambers are distributed along the axis of the tube, and the first chamber is located above the second chamber.

5. A closed consumable according to claim 4, in which the reagent vessel comprises first and second tubular bodies which are releasably sealingly connected, the first chamber being located in the first tubular body and the second chamber being located in the second tubular body.

6. A closed consumable according to claim 1, in which the first and second chambers are radially distributed along the tube, the first chamber having a volume smaller than the volume of the second chamber; when the tube cover covers the reagent tube, a gap for liquid to move from the first chamber to the second chamber is formed between the top of the separating piece and the tube cover.

7. A closed consumable according to claim 6, in which the partition is a baffle having an arcuate configuration in radial cross-section through the reagent tube, the arcuate configuration having an arc centre in the direction of the first chamber.

8. A closed consumable according to any one of claims 1 to 7, in which the external force application state is a centrifugal state.

9. A detection kit, comprising a kit body, a lid body and a plurality of the closed consumables according to any one of claims 1 to 8, wherein the reagent tubes of the closed consumables are integrated into the kit body in an array, and the tube covers corresponding to the reagent tubes one by one are integrated into the lid body.

10. The detecting box according to claim 9, wherein the abutting surface of the box body and the cover is an inclined surface.